CN111492972A - Rooting culture medium for cymbidium hybridum - Google Patents

Rooting culture medium for cymbidium hybridum Download PDF

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Publication number
CN111492972A
CN111492972A CN202010335161.1A CN202010335161A CN111492972A CN 111492972 A CN111492972 A CN 111492972A CN 202010335161 A CN202010335161 A CN 202010335161A CN 111492972 A CN111492972 A CN 111492972A
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stirring
mixing
bin
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storehouse
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闻智磊
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Anhui Zhiye Biotechnology Development Co ltd
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Anhui Zhiye Biotechnology Development Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture

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Abstract

The invention discloses a rooting culture medium for cymbidium hybridum, which comprises 2/3MS culture medium, 25-30 g/L g of sugar, 200 g/L g of mashed potato, 10-15 mg/L of NAA, 5-10 mg/L of IBA, 2 g/L of activated carbon and 5-10 g/L of agar.

Description

Rooting culture medium for cymbidium hybridum
Technical Field
The invention belongs to the technical field of orchid culture, and particularly relates to a rooting culture medium for cymbidium hybridum.
Background
Orchid, Chinese traditional famous flower, the germplasm resource is abundant, distribute extensively, and have higher ornamental value, its propagation method is generally to propagate for the plant division, can go on once in 3-4 years, the propagation speed is slower, difficult to meet the market demand.
Traditional plant rooting medium is when carrying out the cultivation of the cymbidium hybridum, the rooting rate of the cymbidium hybridum is lower, the root length is shorter, the growth speed is slow, the cultivated cymbidium hybridum bud is thin and small, the leaf color is yellow, and then the quality of the cymbidium hybridum is influenced, and in the preparation process of the rooting medium, because of the need of complete manual operation, the preparation efficiency of the medium is greatly reduced, and because the temperature of the culture solution is higher in the preparation process, personnel scald is easily caused.
Disclosure of Invention
The invention aims to provide a rooting culture medium for cymbidium hybridum.
The technical problems to be solved by the invention are as follows:
traditional plant rooting medium is when carrying out the cultivation of the cymbidium hybridum, the rooting rate of the cymbidium hybridum is lower, the root length is shorter, the growth speed is slow, the cultivated cymbidium hybridum bud is thin and small, the leaf color is yellow, and then the quality of the cymbidium hybridum is influenced, and in the preparation process of the rooting medium, because of the need of complete manual operation, the preparation efficiency of the medium is greatly reduced, and because the temperature of the culture solution is higher in the preparation process, personnel scald is easily caused.
The purpose of the invention can be realized by the following technical scheme:
a rooting culture medium for cymbidium hybridum comprises 2/3MS culture medium, sugar 25-30 g/L, mashed potato 200 g/L, NAA 10-15 mg/L, IBA 5-10 mg/L, activated carbon 2 g/L, and agar 5-10 g/L;
the preparation steps of the rooting medium are as follows:
step S1: weighing corresponding components according to the formula;
step S2: placing a conical flask in a carrying groove, placing a carrying plate on a support, adding the MS culture medium and agar weighed in the step S1 into a first stirring bin, adding the sugar, mashed potato, NAA, IBA and activated carbon weighed in the step S1 into a second stirring bin, starting a motor and a heater to heat the mixing bin, the first stirring bin and the second stirring bin, driving a mixing shaft to rotate by the motor, driving a first mixing blade, a second mixing blade and a main driving wheel to rotate, driving a first auxiliary driving wheel and a second auxiliary driving wheel to rotate by the main driving wheel through a first driving belt and a second driving belt, enabling the first stirring blade and the second stirring blade to work, completing melting and mixing of the MS culture medium and the agar, uniformly mixing the sugar, mashed potato, NAA, IBA and the activated carbon, opening an inner valve of a feed opening, and opening an MS culture medium, an agar mixture and the sugar, mashed potato, NAA, IBA, activated carbon, agar, a mixture and agar, Mixing the IBA and the activated carbon mixed mixture, stirring in a mixing bin, adding a sodium hydroxide solution after uniformly stirring until the pH value of the mixed solution reaches 5.5 to prepare a culture solution, starting an air cylinder, driving a support to move upwards by the air cylinder so as to enable a blanking head to be positioned inside the opening of the conical flask, starting a valve inside a blanking through hole, and enabling the culture solution to enter the conical flask from the blanking head for later use;
step S3: and (4) putting the conical flask obtained in the step S2 into a sterilization pot, sterilizing for 15-30min at the temperature of 110-120 ℃, and cooling to room temperature to obtain the rooting medium for the cymbidium hybridum.
Further, the mass fraction of the sodium hydroxide solution in step S3 is 15 to 20%.
Further, the culture solution preparing apparatus of step S2 includes a working chamber, a partition board is fixed inside the working chamber, a plurality of evenly distributed feeding through holes are opened on the partition board, the partition board divides the interior of the working chamber into a mixing chamber and a feeding chamber, a motor is arranged at the center of the top end of the exterior of the mixing chamber, a mixing shaft is fixed at the output end of the motor, the mixing shaft is located inside the mixing chamber, a plurality of evenly distributed first mixing blades and second mixing blades are fixed on the mixing shaft, a main driving wheel is also fixed on the mixing shaft, the main driving wheel is located outside the mixing chamber, the mixing shaft is rotatably connected with the mixing chamber, two first stirring chambers and second stirring chambers are oppositely arranged at the top end of the interior of the mixing chamber, a first stirring shaft is arranged inside the first stirring chamber, a plurality of evenly distributed first stirring blades are fixed on the first stirring shaft, a first auxiliary driving wheel is fixed at the top end of the first stirring shaft, the first auxiliary driving wheel is located outside the first stirring bin, the first stirring shaft is connected with the top wall of the first stirring bin and the top wall of the mixing bin in a rotating mode, the second stirring bin is internally provided with a second stirring shaft, a plurality of uniformly distributed second stirring blades are fixed on the second stirring shaft, a second auxiliary driving wheel is fixed at the top end of the second stirring shaft and located outside the first stirring bin, the second stirring shaft is connected with the top wall of the second stirring bin and the top wall of the mixing bin in a rotating mode, a first driving belt is installed between the first auxiliary driving wheel and the main driving wheel, a second driving belt is installed between the second auxiliary driving wheel and the main driving wheel, the feeding bin is located below the mixing bin, a cylinder is fixed at the center of the inner bottom end of the feeding bin, a cylinder rod is fixed at the output end of the cylinder, a support is fixed at the top end of the cylinder rod.
Furthermore, a plurality of uniformly distributed discharging pipes are fixed on the lower surface of the partition plate, the discharging pipes correspond to the discharging through holes one to one, valves are arranged inside the discharging through holes, and the bottom ends of the discharging pipes are in threaded connection with discharging heads.
Further, the motor on be fixed with the motor frame, motor frame and the outside roof fixed connection who mixes the storehouse, first mixing blade is located second mixing blade top, first mixing blade is located between first stirring storehouse and the second stirring storehouse, second mixing blade is located the below in first stirring storehouse and second stirring storehouse.
Further, the top in first stirring storehouse and second stirring storehouse be equipped with the feed inlet, the bottom in first stirring storehouse and second stirring storehouse all is equipped with the feed opening, the inside valve that is equipped with of feed opening, the lateral wall in feeding storehouse is opened there is the discharge gate, installs the door on the discharge gate, the lateral wall in mixing storehouse, first stirring storehouse, second stirring storehouse all is equipped with the heater.
Further, the lateral wall of support and the inner wall of adding the feed bin laminate mutually, the upper surface of support is equipped with the board groove, the inside draw-in groove that is equipped with two opposition and establishes the setting of board groove.
Further, the upper surface of carrying the thing board open and to have a plurality of evenly distributed to carry the thing groove, carry thing groove and first one-to-one correspondence of unloading, the both sides of carrying the thing board are fixed with the cardboard, the cardboard cooperatees with the draw-in groove, carry the lower surface of thing board and laminate mutually with the bottom surface in board groove.
The invention has the beneficial effects that: the invention adds the MS culture medium in the process of preparing the rooting culture medium of cymbidium hybridum, the MS culture medium has higher inorganic salt and ion concentration, is a more stable ion balance solution, has high nitrate content, has proper amount and proportion of nutrients, can meet the nutrition and physiological needs of plant cells, sugar can be used as a carbon source substance to provide energy for the plant cells, can better adjust the osmotic pressure in the culture medium, can increase the concentration of auxin to cause the formation of xylem of the plant, potato mud can provide glucose for the plant, NAA can promote the division and the expansion of the cells, induce the formation of adventitious roots, further increase fruit setting, prevent fruit drop, change the ratio of female flowers and male flowers, can enter the plant through the leaves and the tender epidermis of the plant, seeds can enter the plant and are guided to the whole plant along with nutrient flow, I BA can promote the growth of main roots of the plant and improve the germination rate, the rooting culture medium prepared by the invention has higher rooting rate and longer root length for culturing cymbidium hybridum, and the growth state of the plant is better, so that the cymbidium hybridum has higher quality; the culture solution preparation device is used in the preparation process, a user can place the conical flask by using a new carrying plate in the process of feeding the conical flask through the arrangement of the carrying plate, the carrying plate can be taken out after feeding is finished, the new carrying plate is directly placed on the support, the time for collecting and placing the conical flask is further reduced, the working efficiency of culture solution preparation is improved, the support is driven by the air cylinder to move upwards, the blanking head is positioned in the opening of the conical flask, the valve in the blanking through hole is opened, the culture solution enters the conical flask from the blanking head, the blanking head is in threaded connection with the blanking pipe, the blanking head can be replaced according to the size of the opening of the conical flask, the culture solution is further prevented from spilling outside the conical flask, and the arrangement of the support and the carrying plate ensures that the assembly of the nutrient solution completely does not need manual operation, avoids scalding of personnel due to the fact that the nutrient solution is excessively scalded, and greatly improves the preparation efficiency of the rooting culture medium for cymbidium hybridum.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view showing the construction of a culture solution preparing apparatus according to the present invention;
FIG. 2 is a schematic view showing the structure of a partition plate in the culture solution preparing apparatus according to the present invention;
FIG. 3 is a plan view of a partition plate in the culture solution preparing apparatus according to the present invention;
FIG. 4 is a plan view of a holder in the culture solution preparing apparatus according to the present invention;
FIG. 5 is a side view of a holder in the culture solution preparing apparatus according to the present invention;
FIG. 6 is a plan view of a carrier plate in the culture solution preparing apparatus according to the present invention.
In the figure: 1. a working bin; 11. a partition plate; 12. a blanking through hole; 13. a discharging pipe; 14. blanking head; 2. a mixing bin; 21. a motor; 22. a mixing shaft; 23. a first mixing blade; 24. a second mixing blade; 25. a main transmission wheel; 3. a first stirring bin; 31. a first stirring shaft; 32. a first stirring blade; 33. a first secondary transmission wheel; 331. a first drive belt; 4. a second stirring bin; 41. a second stirring shaft; 42. a second stirring blade; 43. a second sub-transmission wheel; 431. a second belt; 5. a feeding bin; 51. a cylinder; 52. a cylinder rod; 53. a support; 531. a plate groove; 532. a card slot; 6. a loading plate; 61. a carrying groove; 62. and (4) clamping the board.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A rooting culture medium for cymbidium hybridum comprises 2/3MS culture medium, sugar 25 g/L, mashed potato 200 g/L, NAA 10 mg/L, IBA 5 mg/L, activated carbon 2 g/L, and agar 5 g/L;
the preparation steps of the rooting medium are as follows:
step S1: weighing corresponding components according to the formula;
step S2: adding the components weighed in the step S1 into a culture solution preparation device to prepare a primary culture solution, adding a sodium hydroxide solution into the primary culture solution until the pH value of the primary culture solution reaches 5.5 to prepare a culture solution, and filling the culture solution into a conical flask for later use;
step S3: and (4) putting the conical flask obtained in the step (S2) into a sterilization pot, sterilizing for 30min at the temperature of 120 ℃, and cooling to room temperature to obtain the rooting medium for the cymbidium hybridum.
Example 2
A rooting culture medium for cymbidium hybridum comprises 2/3MS culture medium, 28 g/L sugar, 200 g/L mashed potato, 13 mg/L NAA, 8 mg/L IBA, 2 g/L activated carbon, and 8 g/L agar;
the preparation steps of the rooting medium are as follows:
step S1: weighing corresponding components according to the formula;
step S2: adding the components weighed in the step S1 into a culture solution preparation device to prepare a primary culture solution, adding a sodium hydroxide solution into the primary culture solution until the pH value of the primary culture solution reaches 5.5 to prepare a culture solution, and filling the culture solution into a conical flask for later use;
step S3: and (4) putting the conical flask obtained in the step (S2) into a sterilization pot, sterilizing for 30min at the temperature of 120 ℃, and cooling to room temperature to obtain the rooting medium for the cymbidium hybridum.
Example 3
A rooting culture medium for cymbidium hybridum comprises 2/3MS culture medium, sugar 30 g/L, mashed potato 200 g/L, NAA 15 mg/L, IBA 10 mg/L, activated carbon 2 g/L, and agar 10 g/L;
the preparation steps of the rooting medium are as follows:
step S1: weighing corresponding components according to the formula;
step S2: adding the components weighed in the step S1 into a culture solution preparation device to prepare a primary culture solution, adding a sodium hydroxide solution into the primary culture solution until the pH value of the primary culture solution reaches 5.5 to prepare a culture solution, and filling the culture solution into a conical flask for later use;
step S3: and (4) putting the conical flask obtained in the step (S2) into a sterilization pot, sterilizing for 30min at the temperature of 120 ℃, and cooling to room temperature to obtain the rooting medium for the cymbidium hybridum.
Comparative example 1
The comparative example is a plant rooting culture medium which is common in the market.
The rooting media prepared in examples 1-3 and comparative example 1 were tested and the test results are shown in table 1 below;
and (2) rooting, namely culturing the cymbidium hybridum buds by the culture mediums prepared in the examples 1-3 and the comparative examples 1-3, culturing 20 bottles in each group of experiments, wherein each bottle comprises 5 seedlings, the culture parameters such as illumination time, illumination intensity, temperature control and the like are completely consistent, checking and calculating the rooting rate and the average root length after 30 days, wherein the rooting rate is the number of rooted explants/the number of inoculated explants × 100%, and the average root length is the total length of roots/the total number of roots.
TABLE 1
Figure BDA0002466297610000071
As can be seen from the above Table 1, the rooting culture medium prepared in the examples 1-3 has a rooting rate of 91.74-92.42% for cymbidium hybridum culture, while the rooting rate of the comparative example 1 is only 75.79%, the average root length of the examples 1-3 is 2.18-2.26cm, while the average root length of the comparative example 1 is 1.22cm, the plants of the examples 1-3 have strong and fast growth and normal leaf color, while the plants of the comparative example 1 have thin and small buds, slow growth and yellow leaf color, which indicates that the rooting culture medium prepared in the invention has a higher rooting rate and longer root length for cymbidium hybridum culture, and the growth state of the plants is better, so that the cymbidium hybridum quality is higher.
Referring to fig. 1-6, the apparatus for preparing a culture solution according to the above embodiment includes a working chamber 1, a partition 11 is fixed inside the working chamber 1, a plurality of evenly distributed feeding through holes 12 are formed on the partition 11, the partition 11 divides the interior of the working chamber 1 into a mixing chamber 2 and a feeding chamber 5, a motor 21 is disposed at the center of the top end of the exterior of the mixing chamber 2, a mixing shaft 22 is fixed at the output end of the motor 21, the mixing shaft 22 is located inside the mixing chamber 2, a plurality of evenly distributed first mixing blades 23 and second mixing blades 24 are fixed on the mixing shaft 22, a main driving wheel 25 is further fixed on the mixing shaft 22, the main driving wheel 25 is located outside the mixing chamber 2, the mixing shaft 22 is rotatably connected with the mixing chamber 2, two first stirring chambers 3 and second stirring chambers 4 are disposed at the top end of the interior of the mixing chamber 2, a first stirring shaft 31 is disposed inside the first stirring chamber 3, a plurality of first stirring blades 32 which are uniformly distributed are fixed on the first stirring shaft 31, a first auxiliary driving wheel 33 is fixed at the top end of the first stirring shaft 31, the first auxiliary driving wheel 33 is positioned outside the first stirring bin 3, the first stirring shaft 31 is rotatably connected with the top walls of the first stirring bin 3 and the mixing bin 2, a second stirring shaft 41 is arranged inside the second stirring bin 4, a plurality of second stirring blades 42 which are uniformly distributed are fixed on the second stirring shaft 41, a second auxiliary driving wheel 43 is fixed at the top end of the second stirring shaft 41, the second auxiliary driving wheel 43 is positioned outside the first stirring bin 3, the second stirring shaft 41 is rotatably connected with the top walls of the second stirring bin 4 and the mixing bin 2, a first driving belt 331 is arranged between the first auxiliary driving wheel 33 and the main driving wheel 25, a second driving belt 431 is arranged between the second auxiliary driving wheel 43 and the main driving wheel 25, and the feeding bin 5 is positioned below the mixing bin 2, an air cylinder 51 is fixed at the center of the bottom end in the feeding bin 5, an air cylinder rod 52 is fixed at the output end of the air cylinder 51, a support 53 is fixed at the top end of the air cylinder rod 52, and a loading plate 6 is arranged on the upper surface of the support 53.
The lower surface of the partition plate 11 is fixed with a plurality of uniformly distributed discharging pipes 13, the discharging pipes 13 correspond to the discharging through holes 12 one by one, valves are arranged inside the discharging through holes 12, and the bottom ends of the discharging pipes 13 are in threaded connection with discharging heads 14.
Motor 21 on be fixed with the motor frame, motor frame and the outside roof fixed connection who mixes storehouse 2, first mixing blade 23 is located second mixing blade 24 top, first mixing blade 23 is located between first stirring storehouse 3 and the second stirring storehouse 4, second mixing blade 24 is located the below in first stirring storehouse 3 and the second stirring storehouse 4.
First stirring storehouse 3 and second stirring storehouse 4 top be equipped with the feed inlet, the bottom in first stirring storehouse 3 and second stirring storehouse 4 all is equipped with the feed opening, the inside valve that is equipped with of feed opening, the lateral wall of adding feed bin 5 is opened there is the discharge gate, installs the door on the discharge gate, the lateral wall that mixes storehouse 2, first stirring storehouse 3, second stirring storehouse 4 all is equipped with the heater.
The lateral wall of support 53 and the inner wall of adding feed bin 5 laminate mutually, the upper surface of support 53 is equipped with board groove 531, the inside draw-in groove 532 that is equipped with two opposition and establishes the setting of board groove 531.
The upper surface of carrying board 6 open and to have a plurality of evenly distributed carry thing groove 61, carry thing groove 61 and lower stub bar 14 one-to-one, the both sides of carrying board 6 are fixed with cardboard 62, cardboard 62 cooperatees with draw-in groove 532, carry the lower surface of thing board 6 and laminate mutually with the bottom surface in board groove 531.
Working steps and working principle:
place the erlenmeyer flask in carrying thing groove 61, will carry thing board 6 to place on support 53, the setting that carries thing board 6 makes and is carrying out reinforced in-process to the erlenmeyer flask, the user can use new year thing board 6 to put the erlenmeyer flask, after accomplishing reinforced, it takes out to carry thing board 6, with new year thing board 6 directly place on support 53, and then reduce the time that the erlenmeyer flask was collected and was put, the work efficiency of culture solution preparation has been accelerated, add MS culture medium and agar in first stirring storehouse 3, with sugar, potato mud, NAA, IBA, during active carbon adds second stirring storehouse 4, open motor 21 and open the heater and to mixing storehouse 2, first stirring storehouse 3, second stirring storehouse 4 heats, motor 21 drives mixing shaft 22 and rotates, mixing shaft 22 drives first mixing blade 23, second mixing blade 24, main drive wheel 25 rotates, main drive wheel 25 drives first auxiliary drive wheel 33 and second auxiliary drive wheel 43 through first drive belt 331 and second 431 and commentaries on classics drive wheel 43 Moving to enable the first stirring blade 32 and the second stirring blade 42 to work, melting and mixing MS culture medium and agar, uniformly mixing sugar, mashed potato, NAA, IBA and activated carbon, opening a valve at the inner part of a feed opening, stirring the MS culture medium, agar mixture and sugar, mashed potato, NAA, IBA and activated carbon mixture in a mixing bin 2, adding sodium hydroxide solution after uniform stirring until the pH value of the mixed solution reaches 5.5 to prepare culture solution, opening an air cylinder 51, driving a support 53 to move upwards by the air cylinder 51, enabling a feed head 14 to be located inside the opening of the conical flask, opening a valve inside a feed through hole 12, enabling the culture solution to enter the conical flask from the feed head 14, enabling the feed head 14 and a feed pipe 13 to be replaced according to the size of the opening of the conical flask, further preventing the culture solution from spilling outside the conical flask, arranging the support 53 and a carrying plate 6, the assembly of the nutrient solution completely does not need manual operation, thereby avoiding scalding of personnel due to the excessive scalding of the nutrient solution and greatly improving the preparation efficiency of the rooting culture medium of the cymbidium hybridum.
The foregoing is merely exemplary and illustrative of the principles of the present invention and various modifications, additions and substitutions of the specific embodiments described herein may be made by those skilled in the art without departing from the principles of the present invention or exceeding the scope of the claims set forth herein.

Claims (9)

1. A rooting culture medium for cymbidium hybridum is characterized in that the rooting culture medium comprises 2/3MS culture medium, 25-30 g/L of sugar, 200 g/L of mashed potato, 10-15 mg/L of NAA, 5-10 mg/L of IBA, 2 g/L of activated carbon and 5-10 g/L of agar;
the preparation steps of the rooting medium are as follows:
step S1: weighing corresponding components according to the formula;
step S2: adding the components weighed in the step S1 into a culture solution preparation device to prepare a primary culture solution, adding a sodium hydroxide solution into the primary culture solution until the pH value of the primary culture solution reaches 5.5 to prepare a culture solution, and filling the culture solution into a conical flask for later use;
step S3: and (4) putting the conical flask obtained in the step S2 into a sterilization pot, sterilizing for 15-30min at the temperature of 110-120 ℃, and cooling to room temperature to obtain the rooting medium for the cymbidium hybridum.
2. The rooting medium for cymbidium hybridum according to claim 1, wherein: the mass fraction of the sodium hydroxide solution in the step S3 is 15-20%.
3. The rooting medium for cymbidium hybridum according to claim 1, wherein: the culture solution preparation device comprises a working chamber (1), a partition plate (11) is fixed inside the working chamber (1), a plurality of uniformly distributed blanking through holes (12) are formed in the partition plate (11), the partition plate (11) partitions the interior of the working chamber (1) into a mixing chamber (2) and a feeding chamber (5), a motor (21) is arranged at the center of the top end of the exterior of the mixing chamber (2), a mixing shaft (22) is fixed at the output end of the motor (21), the mixing shaft (22) is located inside the mixing chamber (2), a plurality of uniformly distributed first mixing blades (23) and second mixing blades (24) are fixed on the mixing shaft (22), a main driving wheel (25) is further fixed on the mixing shaft (22), the main driving wheel (25) is located outside the mixing chamber (2), the mixing shaft (22) is rotatably connected with the mixing chamber (2), two first stirring chambers (3) and a second stirring chamber (4) which are oppositely arranged are arranged at the top end of the interior of the mixing chamber (2), a first stirring shaft (31) is arranged in the first stirring bin (3), a plurality of first stirring blades (32) which are uniformly distributed are fixed on the first stirring shaft (31), a first auxiliary driving wheel (33) is fixed at the top end of the first stirring shaft (31), the first auxiliary driving wheel (33) is positioned outside the first stirring bin (3), the first stirring shaft (31) is rotatably connected with the top walls of the first stirring bin (3) and the mixing bin (2), a second stirring shaft (41) is arranged in the second stirring bin (4), a plurality of second stirring blades (42) which are uniformly distributed are fixed on the second stirring shaft (41), a second auxiliary driving wheel (43) is fixed at the top end of the second stirring shaft (41), the second auxiliary driving wheel (43) is positioned outside the first stirring bin (3), and the second stirring shaft (41) is rotatably connected with the top walls of the second stirring bin (4) and the mixing bin (2), install first drive belt (331) between first auxiliary transmission wheel (33) and main drive wheel (25), install second drive belt (431) between second auxiliary transmission wheel (43) and main drive wheel (25), add feed bin (5) and be located the below of mixing storehouse (2), the inside bottom center department of adding feed bin (5) is fixed with cylinder (51), the output of cylinder (51) is fixed with cylinder pole (52), the top of cylinder pole (52) is fixed with support (53), the upper surface of support (53) is equipped with carries thing board (6).
4. The rooting medium for cymbidium hybridum according to claim 3, wherein: the lower surface of the partition board (11) is fixed with a plurality of uniformly distributed discharging pipes (13), the discharging pipes (13) correspond to the discharging through holes (12) one by one, valves are arranged inside the discharging through holes (12), and the bottom ends of the discharging pipes (13) are connected with discharging heads (14) in a threaded mode.
5. The rooting medium for cymbidium hybridum according to claim 3, wherein: motor (21) on be fixed with the motor frame, motor frame and the outside roof fixed connection who mixes storehouse (2), first mixing blade (23) are located second mixing blade (24) top, first mixing blade (23) are located between first stirring storehouse (3) and second stirring storehouse (4), second mixing blade (24) are located the below in first stirring storehouse (3) and second stirring storehouse (4).
6. The rooting medium for cymbidium hybridum according to claim 3, wherein: the top in first stirring storehouse (3) and second stirring storehouse (4) be equipped with the feed inlet, the bottom in first stirring storehouse (3) and second stirring storehouse (4) all is equipped with the feed opening, the feed opening inside is equipped with the valve, the lateral wall of adding feed bin (5) is opened there is the discharge gate, installs the door on the discharge gate, the lateral wall of mixing storehouse (2), first stirring storehouse (3), second stirring storehouse (4) all is equipped with the heater.
7. The rooting medium for cymbidium hybridum according to claim 3, wherein: the lateral wall of support (53) and the inner wall of adding feed bin (5) laminate mutually, the upper surface of support (53) is equipped with board groove (531), inside being equipped with two draw-in grooves (532) of establishing the setting oppositely of board groove (531).
8. The rooting medium for cymbidium hybridum according to claim 7, wherein: the upper surface of carrying thing board (6) open and to have a plurality of evenly distributed carry thing groove (61), carry thing groove (61) and blanking head (14) one-to-one, the both sides of carrying thing board (6) are fixed with cardboard (62), cardboard (62) cooperate with draw-in groove (532), the lower surface that carries thing board (6) is laminated with the bottom surface in board groove (531) mutually.
9. The rooting medium for cymbidium hybridum according to claim 1, wherein: the specific steps of step S2 are as follows:
placing a conical flask in a carrying groove (61), placing a carrying plate (6) on a support (53), adding an MS culture medium and agar into a first stirring bin (3), adding sugar, mashed potato, NAA, IBA and activated carbon into a second stirring bin (4), starting a motor (21) and a heater to heat a mixing bin (2), the first stirring bin (3) and the second stirring bin (4), driving a mixing shaft (22) to rotate by the motor (21), driving a first mixing blade (23), a second mixing blade (24) and a main driving wheel (25) to rotate by the mixing shaft (22), driving a pair of first driving wheel (33) and a pair of second driving wheel (43) to rotate by the main driving wheel (25) through a first driving belt (331) and a second driving belt (431), so that the first mixing blade (32) and the second mixing blade (42) work, and melting and mixing the MS culture medium and the agar, sugar, mashed potato, NAA, IBA, the active carbon misce bene, open the inside valve of feed opening, MS culture medium, agar mixture and sugar, mashed potato, NAA, IBA, the active carbon misce bene and then stir in mixing storehouse (2), after the stirring, add sodium hydroxide solution, reach 5.5 to mixed liquid pH value, make the culture solution, open cylinder (51), cylinder (51) drive support (53) and shift up, and then make feed head (14) be located the erlenmeyer flask bottleneck inside, open the inside valve of unloading through-hole (12), the culture solution gets into the erlenmeyer flask by feed head (14).
CN202010335161.1A 2020-04-24 2020-04-24 Rooting culture medium for cymbidium hybridum Withdrawn CN111492972A (en)

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Application publication date: 20200807