CN110235788A - A kind of preparation method and application of vermiculite plant tissue culture media - Google Patents
A kind of preparation method and application of vermiculite plant tissue culture media Download PDFInfo
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- CN110235788A CN110235788A CN201910709592.7A CN201910709592A CN110235788A CN 110235788 A CN110235788 A CN 110235788A CN 201910709592 A CN201910709592 A CN 201910709592A CN 110235788 A CN110235788 A CN 110235788A
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- vermiculite
- culture media
- plant tissue
- tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
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- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of preparation method of vermiculite plant tissue culture media, 1) vermiculite pre-treatment；2) vermiculite culture medium is prepared；3) post-processing is prepared, the present invention keeps preparing for solid medium more convenient, the cost for reducing production simultaneously, increases shelf lives, while reducing the appearance of mildew risk, facilitate and is accurately adjusted, to make after solid medium sterilization is cooling to be not in that problem really up to the mark or too soft occurs, is conducive to the tissue cultures of plant, improves the chance of success of tissue cultures, use simple, high financial profit.
Description
Technical field
The present invention relates to plant tissue culture media technical field more particularly to a kind of preparations of vermiculite plant tissue culture media
Method and application.
Background technique
In Plant Tissue Breeding, culture medium mainly has solid, liquid and three kinds of semisolid culturemedium.Wherein: liquid training
Supporting base is liquid, adds water to be made into various nutritional ingredients, or be made of the leaching juice (brewer's wort, bean sprout juice etc.) of natural materials；Gu
Body culture medium is in solid-state, is that agar is added in liquid medium as solid culture matrix；Semisolid culturemedium is in scattered paste shape,
It is made of that 0.2~0.5% agar is added in liquid medium.
But in solid culture matrix manufacturing and application, need to be added suitable agar when preparing solid medium,
And the coagulators such as agar is at high cost, can not recycle multiple utilization, and during preparing solid medium, is added each
After plant nutrient liquor, plant growth regulator, carbohydrate, organic nutrition of plant liquid, solid medium itself is easy mildew；Together
When, when preparing solid medium, need to agar (agar can not recycle multiple utilization), macro-element nutrients liquid, micro
The pH value acid solution or lye of the culture solutions such as elemental nutrient liquid, liquid glucose, organic additive matter and hormone are accurately adjusted, such as
The pH value regulation of fruit solid culture base fluid is inaccurate, really up to the mark or too soft after being easy to cause solid medium sterilization cooling,
It is unfavorable for Plant Tissue Breeding, even results in tissue cultures failure.
Summary of the invention
Of the invention provides the preparation method and application of a kind of vermiculite plant tissue culture media.
The scheme of the invention is:
A kind of preparation method of vermiculite plant tissue culture media, including the following steps:
1) vermiculite pre-treatment: vermiculite is cleaned through clear water, is removed soil and sundries, is dried or dry, and 10~50 meshes are crossed
It is spare to obtain thin net vermiculite；
2) vermiculite culture medium is prepared: the plant growth regulator of 0.04~40ml/L, the nutrient solution of 10~200ml/L, 0.1
The fungicide of the ascorbic acid of~5.0g/L, the polyvinylpyrrolidone of 1~5g/L and 1~15g/L is added to step 1) processing
Afterwards in the thin net vermiculite of 760~989.9ml/L, it is uniformly mixed and obtains object vermiculite plant tissue culture media；
3) it prepares post-processing: object vermiculite plant tissue culture media being sub-packed in culture bottle, is sterilized after covering tightly bottle cap
It is spare.
The fungicide includes that Bravo, carbendazim are one such or a variety of mixing are molten as a preferred technical solution,
Liquid.
The step 2) further includes the sucrose of 10~30g/L as a preferred technical solution,.
The invention also discloses a kind of vermiculite plant tissue culture medias as described in claim 1 in liana, draft
Application in plant and woody plant tissure culture.
As a preferred technical solution, the tissue cultures of the liana include liana explant inoculated and cultured,
The culture of liana inducing clumping bud, liana root induction culture.
The liana includes that grape, Kiwi berry are one such as a preferred technical solution,.
As a preferred technical solution, the herbal tissue cultures include herbaceous plant explant inoculated and cultured,
The culture of herbaceous plant inducing clumping bud, herbaceous plant root induction culture.
As a preferred technical solution, the herbaceous plant include Paris polyphylla, it is the bletilla striata, strawberry, radix salviae miltiorrhizae, dendrobium candidum, big
Flower orchid, iris, chrysanthemum.
As a preferred technical solution, the tissue cultures of the xylophyta include xylophyta explant inoculated and cultured,
The culture of xylophyta inducing clumping bud, xylophyta root induction culture.
The xylophyta includes blueberry, cuckoo, camellia, oil tea, paper mulberry as a preferred technical solution,.
By adopting the above-described technical solution, a kind of preparation method of vermiculite plant tissue culture media, 1) vermiculite pre-treatment:
Vermiculite is cleaned through clear water, soil and sundries is removed, dries or dry, it is spare that 10~50 meshes excessively obtain thin net vermiculite；2) leech
Stone culture medium is prepared: the plant growth regulator of 0.04~40ml/L, the nutrient solution of 10~200ml/L, 0.1~5.0g/L it is anti-
The fungicide of bad hematic acid, the polyvinylpyrrolidone of 1~5g/L and 1~15g/L be added to step 1) processing after 760~
In the thin net vermiculite of 989.9ml/L, it is uniformly mixed and obtains object vermiculite plant tissue culture media；3) post-processing is prepared: by mesh
Mark object vermiculite plant tissue culture media is sub-packed in culture bottle, cover tightly sterilize after bottle cap it is spare.
Advantages of the present invention:
Firstly, the present invention keeps preparing for solid medium more convenient, while reducing the cost of production, increases and guarantee the quality
Time, while the appearance of mildew risk is reduced, facilitate and accurately adjusted, thus after keeping solid medium sterilization cooling
Be not in that problem really up to the mark or too soft occurs, be conducive to the tissue cultures of plant, obtain the chance of success of Plant Tissue Breeding
To raising, simple, high financial profit is used.
Specific embodiment
In order to make up the above deficiency, the present invention provides a kind of preparation method of vermiculite plant tissue culture media and application with
Solve the problems in above-mentioned background technique.
A kind of preparation method of vermiculite plant tissue culture media, including the following steps:
1) vermiculite pre-treatment: vermiculite is cleaned through clear water, is removed soil and sundries, is dried or dry, and 10~50 meshes are crossed
It is spare to obtain thin net vermiculite；
2) vermiculite culture medium is prepared: the plant growth regulator of 0.04~40ml/L, the nutrient solution of 10~200ml/L, 0.1
The fungicide of the ascorbic acid of~5.0g/L, the polyvinylpyrrolidone of 1~5g/L and 1~15g/L is added to step 1) processing
Afterwards in the thin net vermiculite of 760~989.9ml/L, it is uniformly mixed and obtains object vermiculite plant tissue culture media；
3) it prepares post-processing: object vermiculite plant tissue culture media being sub-packed in culture bottle, is sterilized after covering tightly bottle cap
It is spare.
The fungicide includes that Bravo, carbendazim be one such or a variety of mixed solutions.
The step 2) further includes the sucrose of 10~30g/L.
The invention also discloses a kind of vermiculite plant tissue culture medias as described in claim 1 in liana, draft
Application in plant and woody plant tissure culture.
The tissue cultures of the liana include liana explant inoculated and cultured, the training of liana inducing clumping bud
It supports, liana root induction culture.
The liana includes that grape, Kiwi berry are one such.
The herbal tissue cultures include herbaceous plant explant inoculated and cultured, the training of herbaceous plant inducing clumping bud
It supports, herbaceous plant root induction culture.
The herbaceous plant includes Paris polyphylla, the bletilla striata, strawberry, radix salviae miltiorrhizae, dendrobium candidum, hybrid cymbidium, iris, chrysanthemum.
The tissue cultures of the xylophyta include xylophyta explant inoculated and cultured, the training of xylophyta inducing clumping bud
It supports, xylophyta root induction culture.
The xylophyta includes blueberry, cuckoo, camellia, oil tea, paper mulberry.
In order to be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, tie below
Specific embodiment is closed, the present invention is further explained.
Embodiment 1:
1) vermiculite pre-treatment: vermiculite is cleaned through clear water, is removed soil and sundries, is dried or dry, and is crossed 10 meshes and is obtained
Thin net vermiculite is spare；
2) vermiculite culture medium prepare: the plant growth regulator of 0.04ml/L, the nutrient solution of 10ml/L, 0.1g/L it is anti-bad
The fungicide of hematic acid, the polyvinylpyrrolidone of 1g/L and 1g/L is added to after step 1) is handled in the thin net vermiculite of 760ml/L,
It is uniformly mixed and obtains object vermiculite plant tissue culture media；
3) it prepares post-processing: object vermiculite plant tissue culture media being sub-packed in culture bottle, is sterilized after covering tightly bottle cap
It is spare.
The fungicide includes that Bravo, carbendazim be one such or a variety of mixed solutions.
The step 2) further includes the sucrose of 10g/L.
Embodiment 2:
1) vermiculite pre-treatment: vermiculite is cleaned through clear water, is removed soil and sundries, is dried or dry, and is crossed 50 meshes and is obtained
Thin net vermiculite is spare；
2) vermiculite culture medium prepare: the plant growth regulator of 40ml/L, the nutrient solution of 200ml/L, 5.0g/L it is anti-bad
The fungicide of hematic acid, the polyvinylpyrrolidone of 5g/L and 15g/L is added to the thin net vermiculite of 989.9ml/L after step 1) processing
In, it is uniformly mixed and obtains object vermiculite plant tissue culture media；
3) it prepares post-processing: object vermiculite plant tissue culture media being sub-packed in culture bottle, is sterilized after covering tightly bottle cap
It is spare.
The fungicide includes that Bravo, carbendazim be one such or a variety of mixed solutions.
The step 2) further includes the sucrose of 30g/L.
Embodiment 3:
1) vermiculite pre-treatment: vermiculite is cleaned through clear water, is removed soil and sundries, is dried or dry, and is crossed 20 meshes and is obtained
Thin net vermiculite is spare；
2) vermiculite culture medium prepare: the plant growth regulator of 20ml/L, the nutrient solution of 100ml/L, 2.0g/L it is anti-bad
The fungicide of hematic acid, the polyvinylpyrrolidone of 2g/L and 10g/L is added to the thin net vermiculite of 800ml/L after step 1) processing
In, it is uniformly mixed and obtains object vermiculite plant tissue culture media；
3) it prepares post-processing: object vermiculite plant tissue culture media being sub-packed in culture bottle, is sterilized after covering tightly bottle cap
It is spare.
The fungicide includes that Bravo, carbendazim be one such or a variety of mixed solutions.
The step 2) further includes the sucrose of 20g/L.
Embodiment 4:
Carry out liana explant inoculated and cultured, the culture of liana inducing clumping bud, liana respectively to grape
Root induction culture, liana explant inoculated and cultured, the culture of liana inducing clumping bud, liana adventitious root lure
Leading the solid medium that culture uses is the vermiculite plant tissue culture media that embodiment 3 is prepared into；
The success of grape explant inoculated and cultured, and cultivate effect is identical as the effect that conventional medium is turned out or class
Seemingly, or even some has exceeded expection；
Grape inducing clumping bud culture success, and cultivate effect is identical as the effect that conventional medium is turned out or class
Seemingly, or even some has exceeded expection；
Grape root induction culture success, and cultivate effect is identical as the effect that conventional medium is turned out or class
Seemingly, or even some has exceeded expection.
Embodiment 5:
It carries out explant inoculated and cultured, inducing clumping bud culture, root induction culture respectively to dendrobium candidum, and uses
The vermiculite plant tissue culture media that embodiment 3 is prepared into；
Dendrobium candidum explant inoculated and cultured success, and cultivate effect it is identical as the effect that conventional medium is turned out or
It is similar, or even some has exceeded expection；
Dendrobium candidum inducing clumping bud culture success, and cultivate effect it is identical as the effect that conventional medium is turned out or
It is similar, or even some has exceeded expection；
Dendrobium candidum root induction culture success, and cultivate effect it is identical as the effect that conventional medium is turned out or
It is similar, or even some has exceeded expection.
Embodiment 6:
Carry out explant inoculated and cultured, inducing clumping bud culture, root induction culture respectively to blueberry, and using implementation
The vermiculite plant tissue culture media that example 3 is prepared into；
The success of blueberry explant inoculated and cultured, and cultivate effect is identical as the effect that conventional medium is turned out or class
Seemingly, or even some has exceeded expection；
Blueberry inducing clumping bud culture success, and cultivate effect is identical as the effect that conventional medium is turned out or class
Seemingly, or even some has exceeded expection；
Blueberry root induction culture success, and cultivate effect is identical as the effect that conventional medium is turned out or class
Seemingly, or even some has exceeded expection.
The above shows and describes the basic principle, main features and advantages of the invention.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent circle.
Claims (10)
1. a kind of preparation method of vermiculite plant tissue culture media, characterized in that it comprises the following steps:
1) vermiculite pre-treatment: vermiculite is cleaned through clear water, is removed soil and sundries, is dried or dry, and is crossed 10~50 meshes and is obtained
Thin net vermiculite is spare；
2) vermiculite culture medium is prepared: the plant growth regulator of 0.04~40ml/L, the nutrient solution of 10~200ml/L, 0.1~
After the fungicide of the ascorbic acid of 5.0g/L, the polyvinylpyrrolidone of 1~5g/L and 1~15g/L is added to step 1) processing
In the thin net vermiculite of 760~989.9ml/L, it is uniformly mixed and obtains object vermiculite plant tissue culture media；
3) prepare post-processing: object vermiculite plant tissue culture media be sub-packed in culture bottle, cover tightly sterilize after bottle cap it is spare.
2. a kind of preparation method of vermiculite plant tissue culture media as described in claim 1, it is characterised in that: the step 2)
It further include the sucrose of 10~30g/L.
3. a kind of preparation method of vermiculite plant tissue culture media as described in claim 1, it is characterised in that: the fungicide
Including Bravo, carbendazim is one such or a variety of mixed solutions.
4. a kind of vermiculite plant tissue culture media as described in claim 1 is in liana, herbaceous plant and xylophyta group
Knit the application in culture.
5. a kind of application of vermiculite plant tissue culture media as claimed in claim 4, it is characterised in that: the liana
Tissue cultures include liana explant inoculated and cultured, the culture of liana inducing clumping bud, liana root induction
Culture.
6. a kind of application of vermiculite plant tissue culture media as claimed in claim 5, it is characterised in that: the liana packet
It is one such to include grape, Kiwi berry.
7. a kind of application of vermiculite plant tissue culture media as claimed in claim 4, it is characterised in that: described herbal
Tissue cultures include herbaceous plant explant inoculated and cultured, the culture of herbaceous plant inducing clumping bud, herbaceous plant root induction
Culture.
8. a kind of application of vermiculite plant tissue culture media as claimed in claim 7, it is characterised in that: the herbaceous plant packet
Include Paris polyphylla, the bletilla striata, strawberry, radix salviae miltiorrhizae, dendrobium candidum, hybrid cymbidium, iris, chrysanthemum.
9. a kind of application of vermiculite plant tissue culture media as claimed in claim 4, it is characterised in that: the xylophyta
Tissue cultures include xylophyta explant inoculated and cultured, the culture of xylophyta inducing clumping bud, xylophyta root induction
Culture.
10. a kind of application of vermiculite plant tissue culture media as claimed in claim 9, it is characterised in that: the xylophyta
Including blueberry, cuckoo, camellia, oil tea, paper mulberry.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111642402A (en) * | 2020-07-20 | 2020-09-11 | 中国农业科学院特产研究所 | Open tissue culture rooting method for actinidia arguta |
| CN112753574A (en) * | 2020-12-30 | 2021-05-07 | 美尚生态景观股份有限公司 | Method for rooting in black pagoda elderberry tissue culture seedling bottle |
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| CN112753574A (en) * | 2020-12-30 | 2021-05-07 | 美尚生态景观股份有限公司 | Method for rooting in black pagoda elderberry tissue culture seedling bottle |
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