CN111357651A - Rooting culture medium for dendrobium huoshanense - Google Patents
Rooting culture medium for dendrobium huoshanense Download PDFInfo
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- CN111357651A CN111357651A CN202010340469.5A CN202010340469A CN111357651A CN 111357651 A CN111357651 A CN 111357651A CN 202010340469 A CN202010340469 A CN 202010340469A CN 111357651 A CN111357651 A CN 111357651A
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Abstract
The invention discloses a rooting culture medium for dendrobium huoshanense, which comprises the following components in formula: 10-15g/L of Huaduo No. 1, 5-10g/L of Huaduo No. 2, 1/2MS culture medium, 25g/L of sugar, 8-15g/L of albumen powder, 50-80g/L of mashed potato, 200ml/L, NAA 5-10mg/L of apple juice, 20-30mg/L, KT 10-15mg/L of 6Ba, 10-15mg/L of 2,4-D, 10-15mg/L of adenine, 2-5g/L of inositol, 2-5g/L of citric acid, 2g/L of activated carbon and 5-10g/L of agar; the rooting culture medium for dendrobium huoshanense has higher rooting rate and longer root length for dendrobium huoshanense bud culture, and the growth state of the plant is better, so that the quality of dendrobium huoshanense is higher; and used a culture solution preparation facilities at the in-process of preparation culture medium, the device makes the assembly of culture solution need not manual operation completely, has avoided scalding the personnel because of nutrient solution is crossed to scald, accomplishes the reinforced back of culture solution, can take out year thing board to directly place new year thing board on the support, and then reduce the erlenmeyer flask and collect the time of putting, accelerated the work efficiency of culture solution preparation.
Description
Technical Field
The invention belongs to the technical field of dendrobium cultivation, and particularly relates to a rooting culture medium for dendrobium huoshanense.
Background
Huoshan rice dendrobium is short for Huoshan dendrobium, is a special variety in Huoshan county, and is an excellent product in dendrobium. However, the propagation coefficient is low, the large-scale production is hindered, and the tissue culture is an important way for solving the problem, but the traditional propagation method is generally the plant division propagation, can be carried out only once in 3-4 years, has a slow propagation speed, and is difficult to meet the market demand.
Traditional plant rooting medium is when carrying out dendrobium huoshanense cultivation, dendrobium huoshanense's rooting rate is lower, the root length is shorter, growth rate is slow, the dendrobium huoshanense bud of cultivateing is thin and small, the leaf color is yellow, and then influenced dendrobium huoshanense's quality, and in the preparation process of rooting medium, because need complete manual operation, make the preparation efficiency greatly reduced of culture medium, and because the culture solution is higher in preparation process temperature, easily cause personnel to scald.
Disclosure of Invention
The invention aims to provide a rooting culture medium for dendrobium huoshanense.
The technical problems to be solved by the invention are as follows:
traditional plant rooting medium is when carrying out dendrobium huoshanense cultivation, dendrobium huoshanense's rooting rate is lower, the root length is shorter, growth rate is slow, the dendrobium huoshanense bud of cultivateing is thin and small, the leaf color is yellow, and then influenced dendrobium huoshanense's quality, and in the preparation process of rooting medium, because need complete manual operation, make the preparation efficiency greatly reduced of culture medium, and because the culture solution is higher in preparation process temperature, easily cause personnel to scald.
The purpose of the invention can be realized by the following technical scheme:
a rooting culture medium for dendrobium huoshanense comprises the following components in percentage by weight: the formula comprises the following components: 10-15g/L of Huaduo No. 1, 5-10g/L of Huaduo No. 2, 1/2MS culture medium, 25g/L of sugar, 8-15g/L of albumen powder, 50-80g/L of mashed potato, 200ml/L, NAA 5-10mg/L of apple juice, 20-30mg/L, KT 10-15mg/L of 6Ba, 10-15mg/L of 2,4-D, 10-15mg/L of adenine, 2-5g/L of inositol, 2-5g/L of citric acid, 2g/L of activated carbon and 5-10g/L of agar;
the preparation steps of the rooting medium are as follows:
step S1: weighing corresponding components according to the formula;
step S2: adding the components weighed in the step S1 into a culture solution preparation device to prepare a primary culture solution, adding a sodium hydroxide solution into the primary culture solution until the pH value of the primary culture solution reaches 5.5 to prepare a culture solution, and filling the culture solution into a conical flask for later use;
step S3: and (4) placing the conical flask obtained in the step S2 into a sterilization pot, sterilizing for 20-30min at the temperature of 100-110 ℃, and cooling to room temperature to obtain a culture medium for inducing proliferation of cymbidium.
Further, the mass fraction of the sodium hydroxide solution in step S3 is 10 to 15%.
Further, step S2, the culture solution preparing apparatus includes a working tank, three pillars are uniformly connected to the outer side of the lower end of the working tank, the bottoms of the three pillars are connected through a base, the left side of the upper end of the working tank is connected with a feed pipe, the upper end of the feed pipe is provided with a cover plate, the cover plate is connected with the feed pipe through a connecting shaft, the middle part of the outer side of the upper end of the working tank is provided with a motor, the shaft extension end of the motor is connected with a stirring shaft, the upper end of the stirring shaft in the working tank is connected with a scraping plate, the scraping plate is connected with the inner wall of the working tank in a sliding manner, the stirring shaft is provided with two stirring rods at the inner extension part of the working tank, the lower end of the stirring rod is provided with a discharging device, the lower end of the discharging device is connected with a, the cylinder is connected to cylinder pole lower extreme, and cylinder lower extreme fixed connection is on the center of base.
Further, discharging device includes the discharging pipe, and the discharging pipe is connected in the bottom of working tank, and a circular baffle is fixed through three supporting legs in the upper end of discharging pipe, and the lower extreme of discharging pipe is equipped with flow control valve.
Furthermore, the sub-packaging device comprises a sub-packaging pipe, the lower end of the sub-packaging pipe is communicated with a plurality of blanking pipes which are uniformly distributed, and the bottom end of each blanking pipe is in threaded connection with a blanking head.
Further, the side wall of the working tank is provided with a heater.
Furthermore, the scraper blade is arranged along the direction of the half inner wall of the working tank, and the scraper blade is in the shape of a half section of the working tank.
Further, the lateral wall of support and three pillar laminating mutually, the upper surface of support is equipped with the board groove, the board inslot portion is equipped with the draw-in groove.
Further, the year thing board be circular, the upper surface of carrying the thing board is equipped with a plurality of evenly distributed's year thing groove, carries thing groove and blanking head one-to-one, carries the thing groove and passes through the connecting rod to be connected on carrying the thing board, two end pairs that carry thing inslot wall are provided with different screw thread locking levers in an opposite direction, the one end of screw thread locking lever in carrying the thing inslot is connected with the antiskid ribbed tile, one side of carrying the thing board on carrying the thing groove is fixed with the half-circular arc cardboard, the cardboard cooperatees with the draw-in groove, the lower surface of carrying the thing.
Furthermore, the three supporting legs are arranged at the lower end of the circular partition plate in an equilateral triangle structure, one ends of the supporting legs are welded at the lower end of the circular partition plate, and the other ends of the supporting legs are welded at one end, close to the discharge pipe, of the bottom of the working tank.
The invention has the beneficial effects that:
the MS culture medium is added in the process of preparing the rooting culture medium of the dendrobium huoshanense, the MS culture medium is high in inorganic salt and ion concentration, is a stable ion balance solution, high in nitrate content, and proper in the quantity and proportion of nutrients, can meet the nutritional and physiological needs of plant cells, sugar can be used as a carbon source substance to provide energy for the plant cells, can better adjust osmotic pressure in the culture medium, can increase the concentration of auxin to cause the xylem of the plant to form, apple juice can provide glucose for the plant, 6Ba belongs to cytokinin, can well promote cell division and induce the plant to form new buds, can well help the side buds of the plant to sprout, can break the top advantages, can also promote the normal flowering of short-day plants, can prolong the flowering time of the short-day plants, and can also improve the cold resistance of the plant, NAA can promote cell division and expansion, induce to form adventitious roots, further increase fruit setting, prevent fruit drop, change the female and male flower ratio, can enter the plant through the tender epidermis of the leaf, plant, seed, direct to the whole plant along with nutrient flow, 2,4-D promotes or inhibits some organ growth, make stem base become thick, flower more No. 1 and flower more No. 2 belong to balanced fertilizer and promoting blossom fertilizer respectively, suitable for plant blooming period, growth period, nutrient supplement after the flowering phase, have effects of raising the output, shortening the production period. The rooting culture medium for dendrobium huoshanense prepared by the invention has higher rooting rate and longer root length for dendrobium huoshanense, and the growth state of plants is better, so that the quality of dendrobium huoshanense is higher;
the culture solution preparation device is used in the preparation process, and through the arrangement of the object carrying plate, a user can place the conical flask by using a new object carrying plate in the process of feeding the conical flask, and can take out the object carrying plate after feeding is finished, and directly place the new object carrying plate on the support, so that the time for collecting and placing the conical flask is reduced, the working efficiency of culture solution preparation is improved, different thread locking rods are vertically arranged at two ends of the inner wall of the object carrying groove, and the distance between the two anti-slip plates is adjusted by adjusting the thread locking rods, so that the object carrying size of the object carrying groove is adjusted, the culture solution preparation device can adapt to conical flasks with different sizes, and object carrying grooves with different sizes are not required to be arranged;
the air cylinder drives the support to move upwards, so that the blanking head is positioned inside the opening of the conical flask, the valve inside the blanking through hole is opened, the culture solution enters the conical flask from the blanking head, and the blanking head is in threaded connection with the blanking pipe, so that the blanking head can be replaced according to the size of the opening of the conical flask, and the culture solution is prevented from spilling outside the conical flask; the scraper arranged on the stirring shaft is arranged along the direction of the half inner wall of the working tank, the scraper is in the shape of a half section of the working tank, materials attached to the inner wall surface of the working tank are scraped away, waste of culture solution is reduced, meanwhile, precipitation of the materials on the inner wall of the working tank is avoided, the working tank is cleaned, and the service life of the machine is prolonged; the setting of support and year thing board for the assembly of culture solution does not need manual operation completely, has avoided crossing to scald because of the culture solution and causes personnel to scald, has promoted the preparation efficiency of the rooting medium of huoshan.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view showing the construction of a culture solution preparing apparatus according to the present invention;
FIG. 2 is a schematic view showing the structure of a discharging device in the culture solution preparing apparatus according to the present invention;
FIG. 3 is a schematic view showing the structure of a packaging unit in the culture solution preparing apparatus according to the present invention;
FIG. 4 is a plan view of a partitioning device in the culture solution preparing apparatus according to the present invention;
FIG. 5 is a plan view of a carrier plate in the culture solution preparing apparatus according to the present invention;
FIG. 6 is a side view of a carrier plate in the culture solution preparing apparatus according to the present invention;
FIG. 7 is a side view of a holder in the culture solution preparing apparatus according to the present invention;
FIG. 8 is a plan view of a holder in the culture solution preparing apparatus according to the present invention.
In the figure: 1-working tank, 2-pillar, 3-discharging device, 4-support, 5-cylinder rod, 6-cylinder, 7-base, 8-subpackaging device, 9-stirring shaft, 10-stirring rod, 11-motor, 12-feeding pipe, 13-cover plate, 14 connecting shaft, 15-scraping plate, 16-circular partition plate, 17-supporting leg, 18-discharging pipe, 19-flow control valve, 20-flange, 21-subpackaging pipe, 22-discharging pipe, 23-discharging head, 24-carrying plate, 25-carrying groove, 26-clamping plate, 27-plate groove, 28-clamping groove, 29-threaded locking rod, 30-antiskid plate and 31-connecting rod.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A rooting culture medium for dendrobium huoshanense comprises the following components in percentage by weight: 12g/L of Huaduo No. 1, 6g/L of Huaduo No. 2, 1/2MS culture medium, 25g/L of sugar, 8g/L of albumen powder, 62g/L of mashed potato, 200ml/L, NAA 5mg/L of apple juice, 6Ba25mg/L, KT 13mg/L, 2, 4-D14 mg/L, 11mg/L of adenine, 3g/L of inositol, 3g/L of citric acid, 2g/L of activated carbon and 7g/L of agar;
the preparation steps of the rooting medium are as follows:
step S1: weighing corresponding components according to the formula;
step S2: adding the components weighed in the step S1 into a culture solution preparation device to prepare a primary culture solution, adding a sodium hydroxide solution into the primary culture solution until the pH value of the primary culture solution reaches 5.5 to prepare a culture solution, and filling the culture solution into a conical flask for later use;
step S3: and (4) putting the conical flask obtained in the step (S2) into a sterilizing pot, sterilizing at 105 ℃ for 28min, and cooling to room temperature to obtain a culture medium for inducing proliferation of cymbidium sinense rhizomes.
Example 2
A rooting culture medium for dendrobium huoshanense comprises the following components in percentage by weight: 14g/L of Huaduo No. 1, 13g/L of Huaduo No. 2, 1/2MS culture medium, 25g/L of sugar, 9g/L of albumen powder, 75g/L of mashed potato, 200ml/L, NAA 8g/L of apple juice, 6Ba26mg/L, KT 12mg/L, 2, 4-D13 mg/L, 13mg/L of adenine, 4g/L of inositol, 3g/L of citric acid, 2g/L of activated carbon and 8g/L of agar;
the preparation steps of the rooting medium are as follows:
step S1: weighing corresponding components according to the formula;
step S2: adding the components weighed in the step S1 into a culture solution preparation device to prepare a primary culture solution, adding a sodium hydroxide solution into the primary culture solution until the pH value of the primary culture solution reaches 5.5 to prepare a culture solution, and filling the culture solution into a conical flask for later use;
step S3: and (4) putting the conical flask obtained in the step (S2) into a sterilizing pot, sterilizing for 25min at the temperature of 108 ℃, and cooling to room temperature to obtain a culture medium for inducing proliferation of cymbidium sinense rhizomes.
Example 3
A rooting culture medium for dendrobium huoshanense comprises the following components in percentage by weight: 12g/L of Huaduo No. 1, 9g/L of Huaduo No. 2, 1/2MS culture medium, 25g/L of sugar, 13g/L of albumen powder, 65g/L of mashed potato, 200ml/L, NAA 7mg/L of apple juice, 6Ba28mg/L, KT 12mg/L, 2, 4-D15 mg/L, 14mg/L of adenine, 4g/L of inositol, 3g/L of citric acid, 2g/L of activated carbon and 9g/L of agar;
the preparation steps of the rooting medium are as follows:
step S1: weighing corresponding components according to the formula;
step S2: adding the components weighed in the step S1 into a culture solution preparation device to prepare a primary culture solution, adding a sodium hydroxide solution into the primary culture solution until the pH value of the primary culture solution reaches 5.5 to prepare a culture solution, and filling the culture solution into a conical flask for later use;
step S3: and (4) putting the conical flask obtained in the step (S2) into a sterilizing pot, sterilizing for 26min at the temperature of 101 ℃, and cooling to room temperature to obtain a culture medium for inducing proliferation of cymbidium sinense rhizomes.
Comparative example 1
The comparative example is a plant rooting culture medium which is common in the market.
The rooting media prepared in examples 1-3 and comparative example 1 were tested and the test results are shown in table 1 below;
and (3) rooting, namely culturing dendrobium huoshanense buds through the culture medium prepared in the examples 1-3 and the comparative example 1, culturing 20 bottles in each group of experiment, wherein each bottle comprises 5 culture parameters, the culture parameters such as illumination time, illumination intensity, temperature control and the like are completely consistent, checking and calculating the rooting rate and the average root length after 30 days, wherein the rooting rate is the number of rooted explants/inoculated explants of × 100%, and the average root length is the total length of roots/total number of roots.
TABLE 1
As can be seen from the above Table 1, the rooting medium prepared in examples 1-3 has a rooting rate of 85.23-89.12% for dendrobium huoshanense culture, while the rooting rate of comparative example 1 is only 74.56%, the average root length of examples 1-3 is 2.11-2.20cm, while the average root length of comparative example 1 is 1.34cm, the plants of examples 1-3 have strong and strong growth states, fast growth and normal leaf color, while the plants of comparative example 1 have thin and small buds, slow growth and yellow leaf color, which indicates that the rooting medium prepared in the invention has a higher rooting rate and longer root length for dendrobium huoshanense culture, and the growth state of the plants is better, so that the quality of dendrobium huoshanense is higher.
Referring to fig. 1-8, the apparatus for preparing a culture solution according to the above embodiment includes a working tank 1, three pillars 2 are uniformly connected to the outer side of the lower end of the working tank 1, the bottoms of the three pillars 2 are connected to each other through a base 7, a feeding pipe 12 is connected to the left side of the upper end of the working tank 1, a cover plate 13 is disposed on the upper end of the feeding pipe 12, the cover plate 13 is connected to the feeding pipe 12 through a connecting shaft 14, a motor 11 is mounted in the middle of the outer side of the upper end of the working tank 1, a stirring shaft 9 is connected to a shaft extension end of the motor 11, a scraping plate 15 is connected to the upper end of the stirring shaft 9 in the working tank 1, the scraping plate 15 is slidably connected to the inner wall of the working tank 1, two stirring rods 10 are disposed at the extension portion of the stirring shaft 9 toward the inside of the working, the discharging device 3 is connected with the subpackaging device 8 through a flange 20, a support 4 is arranged at the lower end of the subpackaging device 8, a loading plate 24 is arranged on the upper surface of the support 4, the lower end of the support 4 is connected with a cylinder rod 5, the lower end of the cylinder rod 5 is connected with a cylinder 6, and the lower end of the cylinder 6 is fixedly connected to the center of a base 7;
the discharging device 3 comprises a discharging pipe 18, the discharging pipe 18 is connected to the bottom of the working tank 1, a circular baffle 16 is fixed at the upper end of the discharging pipe 18 through three supporting legs 17, a flow control valve 19 is arranged at the lower end of the discharging pipe 18, a circular partition plate 16 is arranged to guide the flow direction of liquid, the formation of a vortex is broken, the discharging speed of the liquid is accelerated, and time and labor are saved;
the sub-packaging device 8 comprises a sub-packaging pipe 21, the lower end of the sub-packaging pipe 21 is communicated with a plurality of uniformly distributed blanking pipes 22, and the bottom ends of the blanking pipes 22 are in threaded connection with blanking heads 23.
And the side walls of the working tank 1 are provided with heaters.
The three supporting legs 17 are uniformly arranged at the lower end of the circular partition plate 16 in an equilateral triangle structure, one end of each supporting leg 17 is welded at the lower end of the circular partition plate 16, the other end of each supporting leg 17 is welded at one end, close to the discharge pipe 18, of the bottom of the working tank 1, the circular partition plate 16 is fixed by the three supporting legs 17 in a welding mode, and the circular partition plate 16 can stably bear impact force caused by liquid due to the stability of the triangle without influencing the flow speed of the liquid.
The side wall of the support 4 is attached to the three pillars 2, the upper surface of the support 4 is provided with a plate groove 27, and a clamping groove 28 is arranged in the plate groove 27.
The carrying plate 24 is circular, the upper surface of the carrying plate 24 is provided with a plurality of carrying grooves 25 which are uniformly distributed, the carrying grooves 25 correspond to the blanking heads 23 one by one, the carrying grooves 25 are connected to the carrying plate 24 through connecting rods 31, two ends of the inner wall of the carrying grooves 25 are provided with different threaded locking rods 29 in an opposite mode, one ends of the threaded locking rods 29 in the carrying grooves 25 are connected with anti-slip plates 30, the threaded locking rods 29 are adjusted, the carrying sizes of the carrying grooves 25 are adjusted by adjusting the distance between the two anti-slip plates 30, the carrying plate can adapt to conical bottles with different sizes, the carrying grooves 25 with different sizes do not need to be arranged, one side of the carrying plate 24 on the carrying groove 25 is fixed with a semi-circular arc-shaped clamping plate 26, the clamping plate 26 is matched with a clamping groove 28, and the lower surface of the carrying plate.
Working steps and working principle:
placing the conical flask in the loading groove 25, placing the loading plate 24 on the support 4, arranging the loading plate 24 to enable a user to use a new loading plate 24 to place the conical flask in the process of feeding the conical flask, taking out the loading plate 24 after feeding is completed, directly placing the new loading plate 24 on the support 4, further reducing the time for collecting and placing the conical flask, and accelerating the working efficiency of culture solution preparation, during preparation, opening the cover plate 13, firstly adding MS culture medium and agar into the working tank 1, closing the cover plate 13, then starting the motor 11, starting the heater, heating and stirring for 10 minutes, closing the motor 11, opening the cover plate 13, adding Huaduo No. 1, Huaduo No. 2, sugar, protein powder, potato mash, apple juice, NAA, 6Ba, KT, 2,4-D, adenine, inositol, citric acid and activated carbon into the working tank 1, starting a motor 11 to stir and heat, wherein the motor 11 drives a stirring shaft 9 to rotate so as to drive a stirring rod 10 to rotate to stir materials, so that an MS culture medium and agar are melted and mixed firstly, then adding Huaduo No. 1, Huaduo No. 2, sugar, albumen powder, mashed potato, apple juice, NAA, 6Ba, KT, 2,4-D, adenine, inositol, citric acid and active carbon to be uniformly mixed, stirring, adding a sodium hydroxide solution after uniform stirring until the pH value of a mixed solution reaches 5.5 to prepare a culture solution, starting an air cylinder 6, driving a support 4 to move upwards by the air cylinder 6 so as to enable a blanking head 23 to be positioned inside the opening of a conical flask, starting a flow control valve 19 on a discharge pipe 18 to control the flow rate through the flow control valve 19 so as to control the amount of the materials, enabling the culture solution to enter the conical flask from the blanking head 23, and the blanking head 22 to be in threaded connection, make blanking head 23 can change according to the bottleneck size of erlenmeyer flask, and then prevent that the culture solution from unrestrained outside the erlenmeyer flask, support 4 and the setting of carrying thing board 24 for the assembly of culture solution does not need manual operation completely, has avoided causing personnel to scald because of the culture solution is crossed to scald, has promoted the preparation efficiency of the culture medium of Huoshan dendron greatly.
During the period, the scraper 15 is driven to rotate under the rotation of the stirring shaft 9, the scraper 15 continuously slides on the inner wall of the working tank 1, and materials stuck on the inner wall of the working tank 1 in the culture medium preparation process are removed.
The foregoing is merely exemplary and illustrative of the principles of the present invention and various modifications, additions and substitutions of the specific embodiments described herein may be made by those skilled in the art without departing from the principles of the present invention or exceeding the scope of the claims set forth herein.
Claims (11)
1. A rooting culture medium for dendrobium huoshanense is characterized in that: the formula comprises the following components: 10-15g/L of Huaduo No. 1, 5-10g/L of Huaduo No. 2, 1/2MS culture medium, 25g/L of sugar, 8-15g/L of albumen powder, 50-80g/L of mashed potato, 200ml/L, NAA 5-10mg/L of apple juice, 20-30mg/L, KT 10-15mg/L of 6Ba, 10-15mg/L of 2,4-D, 10-15mg/L of adenine, 2-5g/L of inositol, 2-5g/L of citric acid, 2g/L of activated carbon and 5-10g/L of agar;
the preparation steps of the rooting medium are as follows:
step S1: weighing corresponding components according to the formula;
step S2: adding the components weighed in the step S1 into a culture solution preparation device to prepare a primary culture solution, adding a sodium hydroxide solution into the primary culture solution until the pH value of the primary culture solution reaches 5.5 to prepare a culture solution, and filling the culture solution into a conical flask for later use;
step S3: and (4) placing the conical flask obtained in the step S2 into a sterilization pot, sterilizing for 20-30min at the temperature of 100-110 ℃, and cooling to room temperature to obtain a culture medium for inducing proliferation of cymbidium.
2. The rooting medium for dendrobii huoshanense as claimed in claim 1, wherein the rooting medium comprises: the mass fraction of the sodium hydroxide solution in the step S3 is 10-15%.
3. The rooting medium for dendrobii huoshanense as claimed in claim 1, wherein the rooting medium comprises: step S2 the culture solution preparation device, including working tank (1), three pillars (2) are evenly connected to the outer side of the lower end of working tank (1), the bottoms of three pillars (2) are connected through base (7), the left side of the upper end of working tank (1) is connected with feed pipe (12), the upper end of feed pipe (12) is provided with cover plate (13), cover plate (13) is connected with feed pipe (12) through connecting shaft (14), motor (11) is installed in the middle of the outer side of the upper end of working tank (1), the shaft extending end of motor (11) is connected with stirring shaft (9), the upper end of stirring shaft (9) in working tank (1) is connected with a scraping plate (15), scraping plate (15) is connected with the inner wall of working tank (1) in a sliding manner, two stirring rods (10) are arranged at the position where stirring shaft (9) extends to the inside of working tank (1), the lower end of stirring rod, discharging device (3) are connected in the bottom of working tank (1), a partial shipment device (8) is connected to discharging device (3) lower extreme, discharging device (3) are connected through flange (20) with partial shipment device (8), the lower extreme of partial shipment device (8) is equipped with support (4), the upper surface of support (4) is equipped with carries thing board (24), cylinder rod (5) is connected to support (4) lower extreme, cylinder (6) are connected to cylinder rod (5) lower extreme, cylinder (6) lower extreme fixed connection is on the center of base (7).
4. The rooting medium for dendrobii huoshanense as claimed in claim 3, wherein the rooting medium comprises: the discharging device (3) comprises a discharging pipe (18), the discharging pipe (18) is connected to the bottom of the working tank (1), a circular baffle (16) is fixed to the upper end of the discharging pipe (18) through three supporting legs (17), and a flow control valve (19) is arranged at the lower end of the discharging pipe (18).
5. The rooting medium for dendrobii huoshanense as claimed in claim 3, wherein the rooting medium comprises: the sub-packaging device (8) comprises a sub-packaging pipe (21), a plurality of uniformly distributed blanking pipes (22) are communicated with the lower end of the sub-packaging pipe (21), and a blanking head (23) is screwed at the bottom ends of the blanking pipes (22).
6. The rooting medium for dendrobii huoshanense as claimed in claim 3, wherein the rooting medium comprises: the side walls of the working tank (1) are provided with heaters.
7. The rooting medium for dendrobii huoshanense as claimed in claim 3, wherein the rooting medium comprises: the scraper (15) is arranged along the direction of the half inner wall of the working tank (1), and the scraper (15) is in the shape of a half section of the working tank (1).
8. The rooting medium for dendrobii huoshanense as claimed in claim 3, wherein the rooting medium comprises: the side wall of support (4) laminate mutually with three pillar (2), the upper surface of support (4) is equipped with board groove (27), board groove (27) inside is equipped with draw-in groove (28).
9. The rooting medium for dendrobii huoshanense as claimed in claim 3, wherein the rooting medium comprises: the loading plate (24) is circular, the upper surface of the loading plate (24) is provided with a plurality of loading grooves (25) which are uniformly distributed, the loading grooves (25) are in one-to-one correspondence with the blanking heads (23), the loading grooves (25) are connected onto the loading plate (24) through connecting rods (31), two end pairs of the inner wall of each loading groove (25) are provided with different threaded locking rods (29) in an opposite mode, one end of each threaded locking rod (29) in each loading groove (25) is connected with an anti-slip plate (30), one side of each loading plate (24) on each loading groove (25) is fixed with a semi-circular arc-shaped clamping plate (26), each clamping plate (26) is matched with a clamping groove (28), and the lower surface of each loading plate (24) is attached to the bottom surface of each loading.
10. The rooting medium for dendrobii huoshanense as claimed in claim 4, wherein the rooting medium comprises: the three supporting legs (17) are evenly arranged at the lower end of the circular partition plate (16) in an equilateral triangle structure, one ends of the supporting legs (17) are welded at the lower end of the circular partition plate (16), and the other ends of the supporting legs (17) are welded at one end, close to the discharge pipe (18), of the bottom of the working tank (1).
11. The rooting medium for dendrobii huoshanense as claimed in claim 1, wherein the rooting medium comprises: the specific steps of step S2 are as follows:
placing a conical flask in a carrying groove (25), placing a carrying plate (24) on a support (4), during preparation, opening a cover plate (13), adding MS culture medium and agar into a working tank (1), closing the cover plate (13), then starting a motor (11), starting a heater, heating and stirring for 10 minutes, closing the motor (11), opening the cover plate (13), adding Huaduo No. 1, Huaduo No. 2, sugar, protein powder, mashed potato, apple juice, NAA, 6Ba, KT, 2,4-D, adenine, inositol, citric acid and active carbon into the working tank (1), starting the motor (11), stirring and heating, driving a stirring shaft (9) to rotate by the motor (11), thereby driving the stirring rod (10) to rotate, stirring the materials, melting and mixing the MS culture medium and the agar, adding the Huaduo No. 1, stirring and agar, Huaduo No. 2, sugar, albumen powder, mashed potato, apple juice, NAA, 6Ba, KT, 2,4-D, adenine, inositol, citric acid, active carbon misce bene, stir, after the misce bene, add the sodium hydroxide solution, reach 5.5 to mixed solution pH value, make the culture solution, open cylinder (6), cylinder (6) drive support (4) and shift up, and then make blanking head (23) be located inside the erlenmeyer flask bottleneck, open flow control valve (19) on discharging pipe (18), the culture solution is by blanking head (23) entering erlenmeyer flask.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010340469.5A CN111357651A (en) | 2020-04-26 | 2020-04-26 | Rooting culture medium for dendrobium huoshanense |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010340469.5A CN111357651A (en) | 2020-04-26 | 2020-04-26 | Rooting culture medium for dendrobium huoshanense |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111357651A true CN111357651A (en) | 2020-07-03 |
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ID=71199718
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010340469.5A Withdrawn CN111357651A (en) | 2020-04-26 | 2020-04-26 | Rooting culture medium for dendrobium huoshanense |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111357651A (en) |
-
2020
- 2020-04-26 CN CN202010340469.5A patent/CN111357651A/en not_active Withdrawn
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