CN109845642A - A kind of high quality automation cultivating technique of roxburgh anoectochilus terminal bud - Google Patents
A kind of high quality automation cultivating technique of roxburgh anoectochilus terminal bud Download PDFInfo
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Abstract
The present invention proposes a kind of high quality automation cultivating technique of roxburgh anoectochilus terminal bud, breaks through the cultivating technique form of traditional roxburgh anoectochilus terminal bud, the protocorm of chromosome doubling processing is placed in culture medium of growing thickly and continues to cultivate, obtains Multiple Buds；It will be transferred to culture in root media after Multiple Buds plant division and obtain the complete tissue-cultured seedling of root, stem and leaf；Polyploid plant is obtained through screening to tissue-cultured seedling；Healthy and strong at least one plant of target polyploid plant is chosen in polyploid plant；More plants of target seedling are cultivated using this target polyploid plant；Target anoectochilus blume seedling is obtained using the breeding culture that intelligent cultivation mechanism carries out multiple parameters variation to target seedling；After a certain period of time, at least one plant of target anoectochilus blume seedling of robust growth is selected, and records growing environment parameter locating for selected target anoectochilus blume seedling as standard growth environmental parameter.In this way, the present invention allows environment adapted breed, so that the kind being arbitrarily selected is in optimal growth conditions, reach optimal growth quality, can more improve the efficiency of breeding.
Description
Technical field
The present invention relates to plant cultivating method fields, and in particular to a kind of high quality automation cultivating technique of roxburgh anoectochilus terminal bud.
Background technique
The breeding method of polyploid roxburgh anoectochilus terminal bud has been known, as Chinese invention patent CN201510749602.1 discloses one kind
The breeding method of polyploid roxburgh anoectochilus terminal bud kind, comprising the following steps: the seed culture of diploid roxburgh anoectochilus terminal bud (a), is obtained into sprout；
(b), the sprout is immersed in immersion treatment 10-36h in 5-15 μm of ol/L oryzalin solution；(c), the sprout after impregnating continues
Culture, obtains Multiple Buds；(d), it is transferred to culture in root media after the Multiple Buds plant division and obtains the complete tissue culture of root, stem and leaf
Seedling；(e), the tissue-cultured seedling obtains polyploid plant through screening.In step (a) and step (c), the culture is all made of first
Culture medium carries out；First culture medium are as follows: contain BA 0.8-1.2mg/L, KT0.7-1.2mg/L, NAA in MS culture medium
0.05-0.15mg/L, sucrose 20-35g/L, agar 4.5-8g/L, banana 17-25g/L.First culture medium are as follows: MS culture
Contain BA 1.0mg/L, KT1.0mg/L, NAA 0.1mg/L, sucrose 30g/L, agar 5g/L, banana 20g/L in base.In step
(a) in, the time of the culture is 27-40d.In step (b), the concentration of the oryzalin solution is 8-10 μm of ol/L, leaching
Bubble processing 15-30h.The root media are as follows: in MS culture medium containing NAA 0.4-0.8mg/L, active carbon 0.4-0.6g/L,
Banana 18-25g/L.The root media are as follows: contain NAA 0.5mg/L, active carbon 0.5g/L, banana 20g/ in MS culture medium
L.In step (d), the time cultivated in root media is 38-45d.Step (a), (b), (c), (d), (e) are sterile
Under the conditions of carry out；Step (a), (c) and the condition of culture in (d) are equal are as follows: temperature is 25 ± 2 DEG C, humidity 58%-63%, light
It is 1500-2000xl, light application time 9-12h/d according to intensity.In step (b), the sprout impregnate oryzalin solution it
Before, first carry out dark culture 3-5d.
The step of invention, is succinct, but it is similar with other breeding methods in the market, is all to be consolidated growing environment
It is fixed, and the kind of roxburgh anoectochilus terminal bud is chosen, roxburgh anoectochilus terminal bud kind is allowed to adapt to environment, since the factor of environment is varied
, compared to be for roxburgh anoectochilus terminal bud kind it is more complicated changeable, and some environment may a variety of roxburgh anoectochilus terminal buds of negative influence, even if
It is to select the kind for being most suitable for this environment, is actually also detrimental to roxburgh anoectochilus terminal bud growth, and if based on environment
It goes to cultivate new roxburgh anoectochilus terminal bud kind, cost can be greatly improved.So whether can allow environment adapted breed in turn, make any quilt
The kind chosen is in optimal growth conditions, reaches optimal growth quality, can more improve the efficiency of breeding.
In consideration of it, inventor furthers investigate the above problem, there is this case generation then.
Summary of the invention
Environment adapted breed is allowed the purpose of the present invention is to provide one kind, and the kind being arbitrarily selected is made to be in optimal life
Elongate member reaches optimal growth quality, can more improve the efficiency of breeding, and reduces the high quality automation of the roxburgh anoectochilus terminal bud of cost
Cultivating technique.
In order to achieve the above object, the present invention uses such technical solution:
A kind of high quality automation cultivating technique of roxburgh anoectochilus terminal bud, includes the following steps:
(1) by the kind semina of the fruit of diploid roxburgh anoectochilus terminal bud in germination medium, culture obtains protocorm；
(2) chromosome doubling processing then is carried out to the protocorm, inhibit the plant cell of the protocorm has silk
Division, makes the chromosome doubling in plant cell；
(3) protocorm of chromosome doubling processing is placed in culture medium of growing thickly and continues to cultivate, obtain Multiple Buds；
(4) it will be transferred to culture in root media after Multiple Buds plant division and obtain the complete tissue-cultured seedling of root, stem and leaf；
(5) polyploid plant is obtained through screening to tissue-cultured seedling；
(6) healthy and strong at least one plant of target polyploid plant is chosen in polyploid plant；
(7) more plants of target seedling are cultivated using this target polyploid plant；
(8) target roxburgh anoectochilus terminal bud is obtained using the breeding culture that intelligent cultivation mechanism carries out multiple parameters variation to target seedling
Seedling；
After a certain period of time, at least one plant of target anoectochilus blume seedling of robust growth is selected, and is recorded locating for selected target seedling
Growing environment parameter as standard growth environmental parameter；
(9) more plants of target seedling then are cultivated using the same target polyploid plant, then by all target seedling
It is placed in the growing environment of the standard growth environmental parameter and is cultivated.
S1, target polyploid plant is cultivated, after having spent the florescence, picks out a most healthy and strong roxburgh anoectochilus terminal bud and make
For single order roxburgh anoectochilus terminal bud plant, this single order roxburgh anoectochilus terminal bud plant is uniformly cut into multiple single order stem sections；
S2, each single order stem section is respectively placed in single order culture medium and is cultivated, until being trained second order roxburgh anoectochilus terminal bud plant；
S3, multiple healthy and strong second order roxburgh anoectochilus terminal bud plant are selected in multiple second order roxburgh anoectochilus terminal bud plant, then by each health
Second order roxburgh anoectochilus terminal bud plant is all uniformly cut into multiple second order stem sections；
S4, each second order stem section is respectively placed in second order culture medium and is cultivated, until being trained more plants of target seedling.
In the step (7), the multiple stem cells for obtaining target polyploid plant carry out cell culture respectively, until
It is trained more plants of target seedling.
In the step (1), the whole strain of diploid roxburgh anoectochilus terminal bud plant is transplanted in greenhouse in field, is awarded at the florescence
Powder is picked after waiting result and fruit maturation, obtains diploid roxburgh anoectochilus terminal bud fruit.
In the step (8), the intelligent cultivation mechanism includes that multiple cultivation units arranged in parallel are arranged and to cultivation
Unit discharges into the controller of row control；It is described to cultivate the cultivation list that unit row includes multiple edge cultivation unit row's extending direction arrangements
Tuple and multiple air supply devices to each cultivation unit group supply air；
The cultivation unit group includes culture pond, multiple culture buckets that culture medium is contained in culture pond, carrying culture
The cultivation support frame of bucket, in the water installations of applying cultivated below support frame, and the illumination regulating and controlling mechanism of control light；
The bottom of the culture bucket is formed with multiple permeable holes；
The support frame of cultivating includes multiple cultivation sinks being arranged by from coil to coil except interior, each cultivation sink
It is interior to place multiple culture buckets；The cultivation sink successively includes the first cultivation sink, the second cultivation water by inner ring to outer ring
Slot to n-th cultivate sink；The first cultivation sink, the second cultivation sink to the n-th cultivation sink are got higher step by step；
The pool wall of the culture pond is equipped with the fixing piece connecting with the n-th culture platform；
The water installations of applying include that master applies waterpipe, multiple to give water pipe with what each cultivation sink connected one to one
Road and to the main water supply device applying waterpipe and supplying water；The upper end for giving waterpipe is equipped under the cultivation sink
The faucet at end, the lower end for cultivating sink are formed with the through hole connecting with the faucet；Waterpipe is given described in each
On be equipped with the water flow solenoid valve controlled by the controller；
The illumination regulating and controlling mechanism includes the light-transmitting plate for being horizontally placed at the culture pond upper end, is set to culture pond upper edge
Main inclination reflective mirror, drive it is main inclination reflective mirror movement adjusting driving device, be set to it is described first cultivate sink inner ring simultaneously
The conical reflecting mirror and multiple correspond upwardly extended is set to the outer peripheral secondary inclination reflective mirror of each cultivation sink；It is described
Main inclination reflective mirror is gradually obliquely installed towards sun present position from the bottom to top, and the conical reflecting mirror gradually becomes from the bottom to top
Carefully, the secondary inclination reflective mirror is gradually inclined outwardly setting from the bottom to top；The adjusting driving device includes that the master is driven to incline
The overturning drive device that oblique reflective mirror is spun upside down, and the horizontal drive apparatus that the main inclination reflective mirror of driving horizontally rotates；It is described
Overturning drive device includes the horizontal rotating shaft connecting with the lower edges parallel of the main inclination reflective mirror, driving horizontal rotating shaft rotation
Overturning motor, and carrying overturning motor the first bearing seat；The horizontal drive apparatus includes driving first carrying
The vertical shaft that seat horizontally rotates drives the horizontal motor of vertical shaft rotation and the second bearing seat of load level motor；
The top edge of second bearing seat and the culture pond links together；
The air supply device includes air cushion case, the air inlet supervisor extended along each culture pond orientation
Road, the exhaust supervisor road parallel with air inlet main pipeline, the air inlet being connected between air inlet main pipeline and the corresponding culture pond
Secondary duct, the outlet secondary duct being connected between corresponding culture pond and exhaust supervisor road, blasts air into air cushion case
Blower, to the heating element that the air in air cushion case is heated, the system freezed to the air in air cushion case
Cold part carries out moisture absorption to the air in air cushion case to the humidifying component that the air in air cushion case is humidified
Hygroscopic part detects the temperature detection part of the air themperature in culture pond in culture pond, is set to detection training in culture pond
The the first Humidity Detection component for educating the air humidity in pond detects the second Humidity Detection of the humidity of culture medium in culture bucket
Component, and to the mixing component that the air in air cushion case is stirred；The air cushion case have in one end with
First gas outlet of the air inlet main pipeline connection, and the first air inlet in the other end；The culture pond have with it is described
Second air inlet of air inlet secondary duct connection, and the second gas outlet being connected to the outlet secondary duct；
The refrigeration part includes the refrigeration switch pipeline being connected to the air cushion case, and connects with refrigeration switch pipeline
Lead to and contain the cold air compression bottle of cooling compressed air；Refrigeration switch pipeline be equipped with controlled by the controller it is cold
Pneumoelectric magnet valve；
The humidifying component includes the water mist spraying head in the air cushion case, and it is outer in air cushion case and with
The water mist kinetic pump of water mist spraying head connection；The water mist kinetic pump is connected to water source, and the water mist kinetic pump is equipped by described
The water mist switch of controller control；
The hygroscopic part includes the moisture absorption switch pipeline being connected to the air cushion case, and connects with moisture absorption switch pipeline
Lead to and contain water compressed air removes water Air compressing bottle；Moisture absorption switch pipeline is equipped to be controlled by the controller
Moisture absorption solenoid valve；
It carries out in breeding incubation, sets in the controller first empty needed for each cultivation unit row to target seedling
The humidity parameter of culture medium in the preset temperature and humidity parameter of gas, and each culture bucket；And adjust each cultivation
The light-transmitting plate quantity in pond adjusts the light transmittance parameter of each culture pond；The culture medium in different cultivation sinks is set to have at least one
A different parameter makes the target seedling in different cultivation sinks be in different growth rings to simulate different growing environments
In border；
Using the temperature in temperature detection part detection culture pond and temperature signal is fed back into controller, it is wet using first
Degree detection part detects the humidity in culture pond and moisture signal is fed back to controller, and the second Humidity Detection component is utilized to detect
Moisture signal is simultaneously fed back to controller by humidity in culture medium；
When the moisture in the culture medium that the second Humidity Detection component detects in culture bucket is lower than setting value, pass through control
Device controls each water flow solenoid valve and accordingly opens, and applies waterpipe using water supply device, master and gives waterpipe into each cultivation sink
It supplies water, water is drawn into culture medium from cultivation sink using the permeable hole of bottom and is mended to target seedling by each culture bucket
Water, and the flow that each water flow electromagnetism valve regulation respectively gives waterpipe is controlled, it realizes in the different culture buckets cultivated in sink
Culture medium has different humidity；
When needing to be aerated the air in culture pond, air is directly pumped into after air cushion case and passes through again by blower
Air is supplied to culture pond by air inlet main pipeline, the air in culture pond is flowed out by exhaust supervisor road, is realized in culture pond
Air is aerated；
When temperature detection part detects the temperature of the air in culture pond lower than setting value, added by controller control
Thermal part starting, carries out the air in air cushion case to be thermally formed hot-air, under the power effect of blower, hot-air warp
Culture pond is entered by air inlet main pipeline to improve the temperature of air in culture pond；
When temperature detection part detects the temperature of the air in culture pond higher than setting value, controlled by controller cold
Pneumoelectric magnet valve is opened, and the cooling air in cold air compression bottle is flowed into air cushion case, and is made in the stirring of mixing component
It is uniformly mixed to form cold air with the lower air with air cushion case, under the power effect of blower, cold air is via air inlet
Main pipeline enters culture pond to reduce the temperature of air in culture pond；
When the first Humidity Detection component detects the humidity of the air in culture pond lower than setting value, pass through controller control
Water mist processed switch is opened, water mist power pump startup, by water mist spraying head into air cushion case atomized water spray, water mist is in mixing component
Stirring action under with the air in air cushion case be uniformly mixed to form humidifying air, under the power effect of blower, humidification
Air enters culture pond via air inlet main pipeline to improve the humidity of air in culture pond；
When the first Humidity Detection component detects the humidity of the air in culture pond higher than setting value, pass through controller control
Moisture absorption solenoid valve processed is opened, and goes in water Air compressing bottle that water air is gone to be flowed into air cushion case, and in mixing component
It is uniformly mixed to form dry air with the air in air cushion case under stirring action, it is dry empty under the power effect of blower
Gas enters culture pond via air inlet main pipeline to reduce the humidity of air in culture pond；
With the variation of position of sun, overturning motor is controlled by controller and horizontal motor matches the main inclination of adjustment instead
The angles and positions of light microscopic make the light of the main inclination mirror reflection sun be radiated at the target seedling in each cultivation sink always
On.
In the step (2), it is immersed in 6-14 μm of ol/L oryzalin solution and impregnates after the protocorm is secretly trained 4 days
Processing 12-30 hours.
In the step (2), the concentration of the oryzalin solution is 9-10 μm of ol/L, immersion treatment 18-20h.
After adopting the above technical scheme, the high quality of roxburgh anoectochilus terminal bud of the invention automates cultivating technique, traditional gold thread is broken through
The cultivating technique form of lotus, by the kind semina of the fruit of diploid roxburgh anoectochilus terminal bud in germination medium, culture obtains protocorm；So
Chromosome doubling processing is carried out to the protocorm afterwards, inhibits the mitosis of the plant cell of the protocorm, keeps plant thin
Chromosome doubling in born of the same parents；The protocorm of chromosome doubling processing is placed in culture medium of growing thickly and continues to cultivate, obtains Multiple Buds；
It will be transferred to culture in root media after Multiple Buds plant division and obtain the complete tissue-cultured seedling of root, stem and leaf；To tissue-cultured seedling through screening obtain it is more
Times body plant, roxburgh anoectochilus terminal bud polyploid plant compare that traditional diploid roxburgh anoectochilus terminal bud is more healthy and stronger, and blade and vein etc. are generouser clear
It is clear；Healthy and strong at least one plant of target polyploid plant is chosen in polyploid plant；It is cultivated using this target polyploid plant
More plants of target seedling, all target seedling all use the polyploid plant of same parent, can make later period each environmental parameter more
It is objective and conducive to growth comparison is carried out, it is not in influence objectivity because of Different Individual difference itself；Utilize intelligent cultivation machine
The breeding culture that structure carries out multiple parameters variation to target seedling obtains target anoectochilus blume seedling, such as adjusts humidity, the week of culture medium
Enclose the parameters such as temperature and humidity, the intensity of illumination of air；After a certain period of time, at least one plant of target roxburgh anoectochilus terminal bud of robust growth is selected
Seedling, and growing environment parameter locating for selected target anoectochilus blume seedling is recorded as standard growth environmental parameter, this standard growth ring
Border parameter is the environmental parameter grown most useful for such roxburgh anoectochilus terminal bud, can refer to this standard growth environmental parameter later and passes through artificially
Each growth parameter(s) in greenhouse is set, the growth efficiency and quality of the roxburgh anoectochilus terminal bud of selected kind are more efficiently improved；Then
More plants of target seedling are cultivated using the same target polyploid plant, it is raw that all target seedling are then placed on the standard
It is cultivated in the growing environment of long environmental parameter.Compared with prior art, the high quality of roxburgh anoectochilus terminal bud of the invention automates training
Technique is educated, allows environment to adapt to roxburgh anoectochilus terminal bud kind, so that the kind being arbitrarily selected is in optimal growth conditions, reach optimal
Growth quality can more improve the efficiency of breeding, and the roxburgh anoectochilus terminal bud of cultivation is more healthy and stronger, and reduces cost.
Detailed description of the invention
Fig. 1 is the first partial schematic cross-sectional view of intelligent cultivation mechanism；
Fig. 2 is the second local schematic cross-sectional view of intelligent cultivation mechanism；
Fig. 3 is the overlooking structure diagram of intelligent cultivation mechanism；
Fig. 4 is the local overlooking structural schematic diagram of intelligent cultivation mechanism.
In figure:
11- cultivates the second gas outlet unit group 111- culture pond 1111- fixing piece 1112- the second air inlet 1113-
112- culture bucket 113- cultivation support frame 1131- cultivation sink 1141- master applies waterpipe 1142- and gives waterpipe
The main inclination reflective mirror 11531- overturning driving dress of 11421- faucet 11422- water flow solenoid valve 1151- light-transmitting plate 1152-
Set the a1- horizontal rotating shaft a2- overturning vertical shaft b2- water of the first bearing seat of motor a3- 11532- horizontal drive apparatus b1-
Flat telegram machine b3- the second bearing seat 1154- conical reflecting mirror 1155- pair tilts reflective mirror
121- air cushion case 122- air inlet main pipeline 123- exhaust supervisor road 124- air inlet secondary duct 125- outlet
Secondary duct 126- blower 127- heating element 1281- refrigeration switch pipeline 12811- cold air solenoid valve 1282- cold air pressure
Contracting bottle 1291- water mist spraying head 12011- moisture absorption switch pipeline 120111- moisture absorption solenoid valve 12012- removes Air compressing bottle
1202- mixing component
10- target anoectochilus blume seedling.
Specific embodiment
In order to further explain the technical solution of the present invention, being described in detail below by specific embodiment.
A kind of high quality automation cultivating technique of roxburgh anoectochilus terminal bud of the invention includes the following steps: as shown in Figs 1-4
(1) by the kind semina of the fruit of diploid roxburgh anoectochilus terminal bud in germination medium, culture obtains protocorm；
(2) chromosome doubling processing then is carried out to protocorm, inhibits the mitosis of the plant cell of protocorm, makes to plant
Chromosome doubling in object cell；
(3) protocorm of chromosome doubling processing is placed in culture medium of growing thickly and continues to cultivate, obtain Multiple Buds；Specifically may be used
To inhibit plant cell using oryzalin solution, dinitroaniline or phosphoamide class after embryo germination is at protocorm
Mitosis, makes the chromosome doubling in plant cell, and preferably with a certain concentration, (9 μm of ol/L are lower than 0.001mg/ squares li
Rice) oryzalin solution, dinitroaniline or phosphoamide class to protocorm impregnate certain time (20 hours), then will be former
Bulb, which continues to be placed on to sprout, to be cultivated to obtain Multiple Buds in culture medium.
(4) it will be transferred to culture in root media after Multiple Buds plant division and obtain the complete tissue-cultured seedling of root, stem and leaf；
(5) polyploid plant is obtained through screening to tissue-cultured seedling, roxburgh anoectochilus terminal bud polyploid plant compares traditional diploid roxburgh anoectochilus terminal bud
More healthy and stronger, blade and vein etc. are generouser clear；
(6) healthy and strong at least one plant of target polyploid plant is chosen in polyploid plant；
(7) more plants of target seedling are cultivated using this target polyploid plant, all target seedling all use same mother
The polyploid plant of body, can make later period each environmental parameter more objective and conducive to growth comparison is carried out, and be not in because of different
Body itself is different and influences objectivity；
(8) target roxburgh anoectochilus terminal bud is obtained using the breeding culture that intelligent cultivation mechanism carries out multiple parameters variation to target seedling
Seedling 10, such as humidity, the temperature and humidity of surrounding air, the intensity of illumination parameter of adjustment culture medium；
After a certain period of time, at least one plant of target anoectochilus blume seedling 10 of robust growth is selected, and records selected target seedling institute
For the growing environment parameter at place as standard growth environmental parameter, this standard growth environmental parameter is raw most useful for such roxburgh anoectochilus terminal bud
Long environmental parameter can refer to this standard growth environmental parameter by each growth parameter(s) in artificial settings greenhouse, more later
Efficiently improve the growth efficiency and quality of the roxburgh anoectochilus terminal bud of selected kind；
(9) more plants of target seedling then are cultivated using the same target polyploid plant, then by all target seedling
It is placed in the growing environment of standard growth environmental parameter and is cultivated.It allows environment to adapt to roxburgh anoectochilus terminal bud kind, makes arbitrarily selected
Kind be in optimal growth conditions, reach optimal growth quality, can more improve the efficiency of breeding, the roxburgh anoectochilus terminal bud of cultivation is more
Add stalwartness, and reduces cost.
Preferably, it in step (7), carries out, S1, target polyploid plant is cultivated, in mistake in accordance with the following steps
After florescence, a most healthy and strong roxburgh anoectochilus terminal bud is picked out as single order roxburgh anoectochilus terminal bud plant, this single order roxburgh anoectochilus terminal bud plant is uniform
Multiple single order stem sections are cut into, all single order roxburgh anoectochilus terminal bud plant for being cultured object and all using same parent can make the later period each
Environmental parameter is more objective and conducive to growth comparison is carried out, and is not in influence objectivity because of Different Individual difference itself；It is excellent
Selection of land takes the stem part of single order roxburgh anoectochilus terminal bud plant to carry out cutting 1-3cm length, it can be ensured that survival rate.It preferably, will be after cutting
Stablizing solution is made after pulverizing in remaining roxburgh anoectochilus terminal bud tissue, then single order stem section is placed in stablizing solution, in low temperature environment
In stablize processing, actual temp is 3-5 DEG C, and the processing time can be 30-60 hours, single order stem section can be made in same density in this way
Suspend mode is carried out for a period of time in liquid, and organization internal liquid is not easy excessively flows out, reparation and organization internal liquid to rupture cell
The recovery etc. of body all has certain help, is conducive to subsequent transplanting；
S2, each single order stem section is respectively placed in single order culture medium and is cultivated, until being trained second order roxburgh anoectochilus terminal bud plant；
Preferably, single order culture medium is MS culture medium, comprising a great number of elements, moderate-element, microelement, sucrose, agar and water, and can
According to addition buffer solution is required, wherein the weight of a great number of elements, moderate-element, microelement and sucrose accounts for MS culture medium gross weight
The 28-30% of amount, sucrose 25-28mg/L, agar 6-7.5mg/L；
A great number of elements includes KNO3(1850-1950mg/L), CaCl2·2H2O (400-500mg/L), KH2PO4(650-
750mg/L), MgSO4·7H2O (350-400mg/L) and NH4NO3(1600-1700mg/L)；
Moderate-element includes ZnSO4·7H2O (8-9mg/L), FeSO4·7H2O (27-28.5mg/L), MnSO4·4H2O
(22-22.5mg/L) and Na2-EDTA·2H2O(7-7.5mg/L)；
Microelement includes Na2MnO4·2H2O (0.24-0.26mg/L), KI (0.82-0.84mg/L), H3BO3(6.2-
6.3mg/L), CuSO4·5H2O (0.024-0.026mg/L) and CoCl2·6H2O(0.24-0.26mg/L)；
And stablizing solution can be added in single order culture medium as auxiliary according to the actual situation, if stablizing solution is
Nutrition；
Preferably, in step s 2, single order stem section is placed in single order culture medium, it is right in the interior that temperature is 25-26 DEG C
Single order stem section is taken full sun, intensity of illumination 1200-1400Lux, cultivates 12-14 days；Then the bright phase of period 1 is carried out
With two months to two half a months of dark phase alternate culture, wherein the bright phase be in the interior that temperature is 26-29 DEG C, single order stem section is taken
15-17 hours, intensity of illumination 2200-2400Lux, the dark phase was 20-23 DEG C of temperature of interior, without illumination；Then will
Seedling is placed in single order later period culture medium the bright phase for carrying out second round and the dark phase cultivates one month, compared to the period 1,
The bright phase in second round gradually heated up every 4-15 days；
Wherein single order later period culture medium includes 35-50 parts of soil, and 10-20 parts of potato, 10-20 parts of apple butter, banana 10-
20 parts, 2-4 parts of mangosteen peel, 1-2 parts of dragon fruit pericarp, 1-2 parts and peptone 1-2 parts of mango；
S3, multiple healthy and strong second order roxburgh anoectochilus terminal bud plant are selected in multiple second order roxburgh anoectochilus terminal bud plant, then by each health
Second order roxburgh anoectochilus terminal bud plant is all uniformly cut into multiple second order stem sections, and the stem length of a single order roxburgh anoectochilus terminal bud plant is limited, unfavorable
In the cutting and culture that carry out big quantity, but in order to realize greater amount of cutting and object culture, the more multi-environment ginseng of Lai Peihe
Single order stem section is carried out after cultivating again, can produce the second order stem section of several times, and then can carry out a large amount of object trainings by several settings
It supports, or even for greater number of Object of Development, more multistage culture can be carried out；
S4, each second order stem section is respectively placed in second order culture medium and is cultivated, until being trained more plants of target seedling, such as
Adjust the parameters such as humidity, the temperature and humidity of surrounding air, the intensity of illumination of culture medium；Preferably, second order culture medium is MS training
Support base, include a great number of elements, moderate-element, microelement, sucrose, agar and water, and can according to require be added buffer solution,
Middle a great number of elements, moderate-element, microelement and sucrose weight account for the 35-40%, sucrose 25- of MS total weight of medium
28mg/L, agar 6-7.5mg/L；
A great number of elements includes KNO3(1850-1950mg/L), CaCl2·2H2O (400-500mg/L), KH2PO4(650-
750mg/L), MgSO4·7H2O (350-400mg/L) and NH4NO3(1600-1700mg/L)；
Moderate-element includes ZnSO4·7H2O (8-9mg/L), FeSO4·7H2O (27-28.5mg/L), MnSO4·4H2O
(22-22.5mg/L) and Na2-EDTA·2H2O(7-7.5mg/L)；
Microelement includes Na2MnO4·2H2O (0.24-0.26mg/L), KI (0.82-0.84mg/L), H3BO3(6.2-
6.3mg/L), CuSO4·5H2O (0.024-0.026mg/L) and CoCl2·6H2O(0.24-0.26mg/L)；
And stablizing solution can be added in second order culture medium as auxiliary according to the actual situation, if stablizing solution is
Nutrition；
Preferably, in step s 4, in the interior that temperature is 25-26 DEG C, single order stem section is taken full sun, intensity of illumination
For 1200-1400Lux, cultivate 12-14 days.
Preferably, in step (7), the multiple stem cells for obtaining target polyploid plant carry out cell culture respectively,
Until being trained more plants of target seedling.The present invention directly utilizes same strain target polyploid plant culture using cell culture technology
Multiple target seedling out, efficiency and consistency are higher, can directly adopt universal culture plant cell method.
Preferably, in step (1), the whole strain of diploid roxburgh anoectochilus terminal bud plant is transplanted in greenhouse in field, the florescence into
Row pollination, picks after waiting result and fruit maturation, obtains diploid roxburgh anoectochilus terminal bud fruit.Then by the fruit of diploid roxburgh anoectochilus terminal bud
Kind semina in culture medium of sprouting, after culture obtains protocorm execute step (2).
Preferably, in step (8), intelligent cultivation mechanism includes that multiple cultivation units arranged in parallel are arranged and to cultivation list
Member discharges into the controller of row control；Cultivating unit row includes multiple along the cultivation unit group for cultivating unit row's extending direction arrangement
11 and it is multiple to it is each cultivation unit group 11 supply air air supply device；
Preferably, cultivating unit group 11 includes culture pond 111, multiple cultures that culture medium is contained in culture pond 111
Bucket 112, the cultivation support frame 113 of carrying culture bucket 112, in the water installations of applying for cultivating 113 lower section of support frame, and control light
The illumination regulating and controlling mechanism of line；
Preferably, the bottom for cultivating bucket 112 is formed with multiple permeable holes；
Preferably, cultivating support frame 113 includes multiple cultivation sinks 1131, Mei Gepei being arranged by from coil to coil except interior
It educates and places multiple culture buckets 112 in sink 1131；It successively includes the first cultivation sink that sink 1131, which is cultivated, by inner ring to outer ring
1131, second the cultivation sink 1131 of sink 1131 to the n-th is cultivated；First, which cultivates sink 1131, second, cultivates sink 1131 to the n-th
Sink 1131 is cultivated to get higher step by step；
Preferably, the pool wall of culture pond 111 is equipped with the fixing piece 1111 connecting with the n-th culture platform；
Preferably, applying water installations includes that master applies waterpipe 1141, multiple to cultivate what sink 1131 connected one to one with each
Give waterpipe 1142 and to the main water supply device applying waterpipe 1141 and supplying water；The upper end for giving waterpipe 1142, which is equipped with, to be passed through
The faucet 11421 for cultivating 1131 lower end of sink is worn, the lower end for cultivating sink 1131 is formed with to be passed through with what faucet 11421 was connect
Perforation；Respectively give the water flow solenoid valve 11422 for being equipped with and being controlled by the controller on waterpipe 1142；
Preferably, illumination regulating and controlling mechanism includes the light-transmitting plate 1151 for being horizontally placed at 111 upper end of culture pond, is set to culture pond
The main inclination reflective mirror 1152 of 111 upper edges, the adjusting driving device for driving main inclination reflective mirror 1152 to act, is set to first
It cultivates 1131 inner ring of sink and the conical reflecting mirror 1154 upwardly extended and multiple correspond is set to each cultivation sink 1131
Outer peripheral secondary inclination reflective mirror 1155；Main inclination reflective mirror 1152 is gradually obliquely installed towards sun present position from the bottom to top,
Conical reflecting mirror 1154 is tapered from the bottom to top, and pair inclination reflective mirror 1155 is gradually inclined outwardly setting from the bottom to top；It adjusts
Driving device includes the overturning drive device 11531 for driving main inclination reflective mirror 1152 to spin upside down, and drives main inclination reflective
The horizontal drive apparatus 11532 that mirror 1152 horizontally rotates；Overturning drive device 11531 include under main inclination reflective mirror 1152
The horizontal rotating shaft a1 of sides aligned parallel connection, the overturning motor a2 of driving horizontal rotating shaft a1 rotation, and carrying overturning motor a2
First bearing seat a3；Horizontal drive apparatus 11532 includes the vertical shaft b1 that the first bearing seat a3 of driving horizontally rotates, and driving is perpendicular
To the second bearing seat b3 of the shaft b1 horizontal motor b2 rotated and load level motor b2；Second bearing seat b3 and cultivation
The top edge in pond 111 links together；
Preferably, air supply device includes air cushion case 121, the air inlet extended along each 111 orientation of culture pond
Main pipeline 122, the exhaust supervisor road 123 parallel with air inlet main pipeline 122 are connected to air inlet main pipeline 122 and corresponding cultivation
Air inlet secondary duct 124 between pond 111, the outlet secondary duct being connected between corresponding culture pond 111 and exhaust supervisor road 123
125, the blower 126 of air, the heating heated to the air in air cushion case 121 are blasted into air cushion case 121
Component 127 carries out the refrigeration part that the air in air cushion case 121 freezes to the air in air cushion case 121
The humidifying component of humidification carries out the hygroscopic part of moisture absorption to the air in air cushion case 121, is set to detection in culture pond 111
The temperature detection part of air themperature in culture pond 111 detects the air humidity in culture pond 111 in culture pond 111
The first Humidity Detection component, the second Humidity Detection component of the humidity of culture medium is detected in culture bucket 112, and to sky
The mixing component 1202 that air in gas baffle-box 121 is stirred；Air cushion case 121 has to be responsible in one end and air inlet
The first gas outlet that road 122 is connected to, and the first air inlet in the other end；Culture pond 111 has to be connected with air inlet secondary duct 124
The second logical air inlet 1112, and the second gas outlet 1113 being connected to outlet secondary duct 125；
Preferably, refrigeration part includes the refrigeration switch pipeline 1281 being connected to air cushion case 121, and is switched with refrigeration
Pipeline 1281 is connected to and contains the cold air compression bottle 1282 of cooling compressed air；Refrigeration switch pipeline 1281, which is equipped, to be controlled
The cold air solenoid valve 12811 of device control；It is loaded in cold air compression bottle 1282 through supercooling compressed air；
Preferably, humidifying component includes the water mist spraying head 1291 in air cushion case 121, and is in air cushion case
The water mist kinetic pump being connected to outside 121 and with water mist spraying head 1291；Water mist kinetic pump is connected to water source, water mist kinetic pump be equipped with by
The water mist switch of controller control；
Preferably, hygroscopic part includes the moisture absorption switch pipeline 12011 being connected to air cushion case 121, and is opened with moisture absorption
Close that pipeline 12011 is connected to and contain water compressed air removes water Air compressing bottle 12012；Moisture absorption switchs pipeline 12011 and is arranged
There is the moisture absorption solenoid valve 120111 being controlled by the controller；It goes in water Air compressing bottle 12012 to be loaded with empty by dry compression
Gas；
Preferably, target seedling is being carried out to set each cultivation unit in the controller first in breeding incubation
The humidity parameter of culture medium in the preset temperature and humidity parameter of air needed for arranging, and each culture bucket 112；And it adjusts
1151 quantity of light-transmitting plate of whole each culture pond 111 adjusts the light transmittance parameter of each culture pond 111；Make different cultivation sinks
There is culture medium in 1131 at least one different parameter to make different cultivation sinks 1131 to simulate different growing environments
In target seedling be in different growing environments；
Using the temperature in temperature detection part detection culture pond 111 and temperature signal is fed back into controller, utilizes the
One Humidity Detection component detects the humidity in culture pond 111 and moisture signal is fed back to controller, utilizes the second Humidity Detection
Component detects the humidity in culture medium and moisture signal is fed back to controller；
When the moisture in the culture medium that the second Humidity Detection component detects in culture bucket 112 is lower than setting value, pass through
Controller controls each water flow solenoid valve 11422 and accordingly opens, and applies waterpipe 1141 using water supply device, master and gives waterpipe
1142 supply water into each cultivation sink 1131, and each bucket 112 of cultivating is inhaled water from cultivation sink 1131 using the permeable hole of bottom
Enter into culture medium and moisturizing is carried out to target seedling, and controls each adjusting of water flow solenoid valve 11422 and respectively give waterpipe 1142
Flow realizes that the culture medium in the different culture buckets 112 cultivated in sink 1131 has different humidity；
When needing to be aerated the air in culture pond 111, air is directly pumped into air cushion case by blower 126
Air is supplied to culture pond 111 via air inlet main pipeline 122 again after 121, the air in culture pond 111 is by exhaust supervisor road 123
Outflow, realization are aerated the air in culture pond 111；
When temperature detection part detects the temperature of the air in culture pond 111 lower than setting value, pass through controller control
Heating element 127 processed starts, and carries out being thermally formed hot-air to the air in air cushion case 121, makees in the power of blower 126
Under, hot-air enters culture pond 111 via air inlet main pipeline 122 to improve the temperature of air in culture pond 111；
When temperature detection part detects the temperature of the air in culture pond 111 higher than setting value, pass through controller control
The pneumoelectric magnet valve 12811 that freezes is opened, and the cooling air in cold air compression bottle 1282 is flowed into air cushion case 121, and
It is uniformly mixed to form cold air with the air in air cushion case 121 under the stirring action of mixing component 1202, in blower 126
Under power effect, cold air enters culture pond 111 via air inlet main pipeline 122 to reduce the temperature of air in culture pond 111；
When the first Humidity Detection component detects the humidity of the air in culture pond 111 lower than setting value, pass through control
Device control water mist switch open, water mist power pump startup, by water mist spraying head 1291 into air cushion case 121 atomized water spray, water
Mist is uniformly mixed to form humidifying air with the air in air cushion case 121 under the stirring action of mixing component 1202, in wind
Under the power effect of machine 126, humidifying air enters culture pond 111 via air inlet main pipeline 122 to improve air in culture pond 111
Humidity；
When the first Humidity Detection component detects the humidity of the air in culture pond 111 higher than setting value, pass through control
Device controls moisture absorption solenoid valve 120111 and opens, and goes in water Air compressing bottle 12012 that water air is gone to be flowed into air cushion case 121
In, and it is uniformly mixed to form dry air with the air in air cushion case 121 under the stirring action of mixing component 1202,
Under the power effect of blower 126, dry air is hollow to reduce culture pond 111 into culture pond 111 via air inlet main pipeline 122
The humidity of gas；
With the variation of position of sun, adjustment master is matched by controller control overturning motor a2 and horizontal motor b2 and is inclined
The angles and positions of oblique reflective mirror 1152, the light for making main inclination reflective mirror 1152 reflect the sun are radiated at each cultivation sink always
On target seedling in 1131.Main inclination reflective mirror 1152 by light, simultaneously will on rooted plantlet directly down by reflected illumination
Light is reflected to conical reflecting mirror 1154 and secondary inclination reflective mirror 1155, then by conical reflecting mirror 1154 and secondary inclination reflective mirror
1155 by light from side to be reflected to rooted plantlet from the bottom to top, make rooted plantlet by comprehensive illumination, growth and development
More comprehensively, Leaf positional distribution is uniform.
Preferably, heating element 127 includes the electrical heating element in air cushion case 121, and to electrical heating element
The computer heating control switch for being switched and being controlled by the controller；Electrical heating element includes adding for multilayer vertical air baffle-box 121
Air cushion case 121 is separated into the first buffer chamber and the second buffer chamber by heat penetration gas metal mesh, electrical heating element.The present invention
In practical work process, controlling computer heating control switch by controller makes electrical heating element heating power, to air cushion case
It carries out being thermally formed hot-air by heating the outside air of Vented metal net in 121.Preferably, the second buffer chamber is than first
Buffer chamber closer to cultivate unit group 11.
Preferably, mixing component 1202 includes the stirring blade set on the first cushion chamber falls, and driving stirring blade turns
Dynamic stirring drive rod, and the stirring for driving stirring drive rod rotation outside air cushion case 121 and being controlled by the controller
Driving motor；It is parallel with the extending direction of air cushion case 121 to stir drive rod.
Preferably, it in step (2), is immersed in 6-14 μm of ol/L oryzalin solution and impregnates after protocorm secretly being trained 4 days
Processing 12-30 hours.
Preferably, in step (2), the concentration of oryzalin solution is 9-10 μm of ol/L, immersion treatment 18-20h.
Product form of the invention is not limited to this case diagram and embodiment, anyone carries out the appropriate of similar thinking to it
Changes or modifications all should be regarded as not departing from patent category of the invention.
Claims (7)
1. a kind of high quality of roxburgh anoectochilus terminal bud automates cultivating technique, which comprises the steps of:
(1) by the kind semina of the fruit of diploid roxburgh anoectochilus terminal bud in germination medium, culture obtains protocorm；
(2) chromosome doubling processing then is carried out to the protocorm, inhibits the mitosis of the plant cell of the protocorm,
Make the chromosome doubling in plant cell；
(3) protocorm of chromosome doubling processing is placed in culture medium of growing thickly and continues to cultivate, obtain Multiple Buds；
(4) it will be transferred to culture in root media after Multiple Buds plant division and obtain the complete tissue-cultured seedling of root, stem and leaf；
(5) polyploid plant is obtained through screening to tissue-cultured seedling；
(6) healthy and strong at least one plant of target polyploid plant is chosen in polyploid plant；
(7) more plants of target seedling are cultivated using this target polyploid plant；
(8) target anoectochilus blume seedling is obtained using the breeding culture that intelligent cultivation mechanism carries out multiple parameters variation to target seedling；
After a certain period of time, at least one plant of target anoectochilus blume seedling of robust growth is selected, and is recorded locating for selected target anoectochilus blume seedling
Growing environment parameter as standard growth environmental parameter；
(9) more plants of target seedling then are cultivated using the same target polyploid plant, then places all target seedling
It is cultivated in the growing environment of the standard growth environmental parameter.
2. a kind of high quality of roxburgh anoectochilus terminal bud according to claim 1 automates cultivating technique, it is characterised in that: in the step
Suddenly in (7),
S1, target polyploid plant is cultivated, after having spent the florescence, picks out a most healthy and strong roxburgh anoectochilus terminal bud as one
This single order roxburgh anoectochilus terminal bud plant is uniformly cut into multiple single order stem sections by rank roxburgh anoectochilus terminal bud plant；
S2, each single order stem section is respectively placed in single order culture medium and is cultivated, until being trained second order roxburgh anoectochilus terminal bud plant；
S3, multiple healthy and strong second order roxburgh anoectochilus terminal bud plant are selected in multiple second order roxburgh anoectochilus terminal bud plant, then by each healthy second order
Roxburgh anoectochilus terminal bud plant is all uniformly cut into multiple second order stem sections；
S4, each second order stem section is respectively placed in second order culture medium and is cultivated, until being trained more plants of target seedling.
3. a kind of high quality of roxburgh anoectochilus terminal bud according to claim 1 automates cultivating technique, it is characterised in that: in the step
Suddenly in (7), the multiple stem cells for obtaining target polyploid plant carry out cell culture respectively, until being trained more plants of target childrens
Seedling.
4. a kind of high quality of roxburgh anoectochilus terminal bud according to claim 1 automates cultivating technique, it is characterised in that: in the step
Suddenly in (1), the whole strain of diploid roxburgh anoectochilus terminal bud plant is transplanted in greenhouse in field, is pollinated at the florescence, wait result and fruit
It is picked after real maturation, obtains diploid roxburgh anoectochilus terminal bud fruit.
5. a kind of high quality of roxburgh anoectochilus terminal bud according to claim 1 automates cultivating technique, it is characterised in that: in the step
Suddenly in (8), the intelligent cultivation mechanism includes multiple cultivation unit rows arranged in parallel and discharges into row control to unit is cultivated
Controller；It is described to cultivate unit row including multiple along the cultivation unit groups for cultivating unit row's extending direction arrangement and multiple to each
Cultivate the air supply device of unit group supply air；
The cultivation unit group includes culture pond, multiple culture buckets that culture medium is contained in culture pond, carrying culture bucket
Support frame is cultivated, in the water installations of applying cultivated below support frame, and the illumination regulating and controlling mechanism of control light；
The bottom of the culture bucket is formed with multiple permeable holes；
The support frame of cultivating includes multiple cultivation sinks being arranged by from coil to coil except interior, is put in each cultivation sink
Set multiple culture buckets；The cultivation sink by inner ring to outer ring successively include the first cultivation sink, second cultivate sink extremely
N-th cultivates sink；The first cultivation sink, the second cultivation sink to the n-th cultivation sink are got higher step by step；
The pool wall of the culture pond is equipped with the fixing piece connecting with the n-th culture platform；
It is described apply water installations include it is main apply waterpipe, it is multiple with it is each it is described cultivate that sink connects one to one give waterpipe and
To the main water supply device applying waterpipe and supplying water；The upper end for giving waterpipe is equipped with through the cultivation sink lower end
Faucet, the lower end for cultivating sink are formed with the through hole connecting with the faucet；It is given described in each on waterpipe
Equipped with the water flow solenoid valve controlled by the controller；
The illumination regulating and controlling mechanism includes the light-transmitting plate for being horizontally placed at the culture pond upper end, set on the master of culture pond upper edge
Reflective mirror is tilted, the adjusting driving device of main inclination reflective mirror movement is driven, is set to described first and cultivates sink inner ring and upward
The conical reflecting mirror of extension and multiple correspond are set to the outer peripheral secondary inclination reflective mirror of each cultivation sink；The master inclines
Oblique reflective mirror is gradually obliquely installed towards sun present position from the bottom to top, and the conical reflecting mirror is tapered from the bottom to top, institute
It states secondary inclination reflective mirror and is gradually inclined outwardly setting from the bottom to top；The adjusting driving device includes driving the main inclination reflective
The overturning drive device that mirror is spun upside down, and the horizontal drive apparatus that the main inclination reflective mirror of driving horizontally rotates；The overturning is driven
Dynamic device includes the horizontal rotating shaft connecting with the lower edges parallel of the main inclination reflective mirror, the overturning of driving horizontal rotating shaft rotation
Motor, and the first bearing seat of carrying overturning motor；The horizontal drive apparatus includes driving first bearing seat horizontal
The vertical shaft of rotation drives the horizontal motor of vertical shaft rotation and the second bearing seat of load level motor；Described
The top edge of two bearing seats and the culture pond links together；
The air supply device includes air cushion case, the air inlet main pipeline extended along each culture pond orientation, with
The parallel exhaust supervisor road of air inlet main pipeline, the air inlet looped pipeline being connected between air inlet main pipeline and the corresponding culture pond
Road, the outlet secondary duct being connected between corresponding culture pond and exhaust supervisor road, the blower of air is blasted into air cushion case,
To the heating element that the air in air cushion case is heated, the refrigeration section freezed to the air in air cushion case
Part carries out the moisture absorption of moisture absorption to the air in air cushion case to the humidifying component that the air in air cushion case is humidified
Component detects the temperature detection part of the air themperature in culture pond in culture pond, is set in culture pond and detects culture pond
First Humidity Detection component of interior air humidity detects the second humidity detection unit of the humidity of culture medium in culture bucket
Part, and to the mixing component that the air in air cushion case is stirred；The air cushion case, which has, is in one end and institute
State the first gas outlet of air inlet main pipeline connection, and the first air inlet in the other end；The culture pond have with it is described into
Second air inlet of gas secondary duct connection, and the second gas outlet being connected to the outlet secondary duct；
The refrigeration part includes the refrigeration switch pipeline being connected to the air cushion case, and is connected to simultaneously with refrigeration switch pipeline
Contain the cold air compression bottle of cooling compressed air；The refrigeration switch pipeline is equipped with the cold air electricity controlled by the controller
Magnet valve；
The humidifying component includes the water mist spraying head in the air cushion case, and outside air cushion case and and water mist
The water mist kinetic pump of spray head connection；The water mist kinetic pump is connected to water source, and the water mist kinetic pump is equipped by the control
The water mist switch of device control；
The hygroscopic part includes the moisture absorption switch pipeline being connected to the air cushion case, and is connected to simultaneously with moisture absorption switch pipeline
Contain go water compressed air remove water Air compressing bottle；The moisture absorption switch pipeline is equipped with the moisture absorption controlled by the controller
Solenoid valve；
It is carried out in breeding incubation to target seedling, sets air needed for each cultivation unit is arranged in the controller first
The humidity parameter of culture medium in preset temperature and humidity parameter, and each culture bucket；And adjust each culture pond
Light-transmitting plate quantity adjusts the light transmittance parameter of each culture pond；Make the culture medium in different cultivation sinks that there is at least one not
Same parameter is in the target seedling in different cultivation sinks in different growing environments to simulate different growing environments；
Using the temperature in temperature detection part detection culture pond and temperature signal is fed back into controller, is examined using the first humidity
It surveys component to detect the humidity in culture pond and moisture signal is fed back to controller, utilizes the second Humidity Detection component to detect and cultivate
Moisture signal is simultaneously fed back to controller by humidity in base；
When the moisture in the culture medium that the second Humidity Detection component detects in culture bucket is lower than setting value, pass through controller control
It makes each water flow solenoid valve accordingly to open, applies waterpipe using water supply device, master and give waterpipe and supply water into each cultivation sink,
Water is drawn into culture medium from cultivation sink using the permeable hole of bottom and carries out moisturizing to target seedling by each culture bucket, and is controlled
The flow that each water flow electromagnetism valve regulation respectively gives waterpipe is made, realizes the culture medium tool in the different culture buckets cultivated in sink
There is different humidity；
When needing to be aerated the air in culture pond, blower directly by air be pumped into after air cushion case again via into
Gas main pipeline supplies air to culture pond, and the air in culture pond is flowed out by exhaust supervisor road, realizes to the air in culture pond
It is aerated；
When temperature detection part detects the temperature of the air in culture pond lower than setting value, heating part is controlled by controller
Part starting, the air in air cushion case is carried out to be thermally formed hot-air, blower power effect under, hot-air via into
Gas main pipeline enters culture pond to improve the temperature of air in culture pond；
When temperature detection part detects the temperature of the air in culture pond higher than setting value, passes through controller and control cold air electricity
Magnet valve is opened, and the cooling air in cold air compression bottle is flowed into air cushion case, and under the stirring action of mixing component
It is uniformly mixed to form cold air with the air in air cushion case, under the power effect of blower, cold air is responsible for via air inlet
Road enters culture pond to reduce the temperature of air in culture pond；
When the first Humidity Detection component detects the humidity of the air in culture pond lower than setting value, water is controlled by controller
Mist switch is opened, and water mist power pump startup, by water mist spraying head, into air cushion case, atomized water spray, water mist are stirred mixing component
The effect of mixing is lower to be uniformly mixed to form humidifying air with the air in air cushion case, under the power effect of blower, humidifying air
Enter culture pond via air inlet main pipeline to improve the humidity of air in culture pond；
When the first Humidity Detection component detects the humidity of the air in culture pond higher than setting value, passes through controller and control suction
Wet solenoid valve is opened, and goes in water Air compressing bottle that water air is gone to be flowed into air cushion case, and in the stirring of mixing component
Effect is lower to be uniformly mixed to form dry air with the air in air cushion case, under the power effect of blower, dry air warp
Culture pond is entered by air inlet main pipeline to reduce the humidity of air in culture pond；
With the variation of position of sun, overturning motor is controlled by controller and horizontal motor matches the main inclination reflective mirror of adjustment
Angles and positions, make it is main inclination the mirror reflection sun light be radiated at always it is each cultivation sink in target seedling on.
6. a kind of high quality of roxburgh anoectochilus terminal bud according to claim 1 automates cultivating technique, it is characterised in that: in the step
Suddenly it in (2), is immersed in after the protocorm is secretly trained 4 days in 6-14 μm of ol/L oryzalin solution immersion treatment 12-30 hours.
7. a kind of high quality of roxburgh anoectochilus terminal bud according to claim 6 automates cultivating technique, it is characterised in that: in the step
Suddenly in (2), the concentration of the oryzalin solution is 9-10 μm of ol/L, immersion treatment 18-20h.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112493132A (en) * | 2020-12-17 | 2021-03-16 | 刘泽洋 | Method for improving gas microenvironment of plant tissue culture bottle seedlings and special culture medium |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007086928A3 (en) * | 2006-01-12 | 2010-08-26 | Cets, Llc | Controlled environment system and method for rapid propagation of seed potato stocks |
| CN103336517A (en) * | 2013-07-02 | 2013-10-02 | 周庆芬 | Intelligent agricultural integrated management system |
| CN103853191A (en) * | 2012-11-30 | 2014-06-11 | 陕西科林能源发展股份有限公司 | Intelligent sunshine directional reflecting device |
| WO2015136926A1 (en) * | 2014-03-14 | 2015-09-17 | パナソニックＩｐマネジメント株式会社 | Plant cultivation apparatus |
| CN206181930U (en) * | 2016-09-09 | 2017-05-24 | 福建虎伯寮生物集团有限公司 | Gold thread lotus intelligence planting system |
| CN106718723A (en) * | 2016-11-24 | 2017-05-31 | 宣威市绿隆农业有限公司 | A kind of nuisanceless high-yield planting method of roxburgh anoectochilus terminal bud |
| CN108094199A (en) * | 2017-12-14 | 2018-06-01 | 泉州市金草生物技术有限公司 | A kind of roxburgh anoectochilus terminal bud cultivating technique |
| CN106508661B (en) * | 2016-11-01 | 2018-07-24 | 玉林师范学院 | A method of creating roxburgh anoectochilus terminal bud polyploid resource |
| CN108684532A (en) * | 2018-08-13 | 2018-10-23 | 儒兰（福建）生物科技有限公司 | A kind of production technology of polyploid roxburgh anoectochilus terminal bud culture medium and polyploid roxburgh anoectochilus terminal bud |
-
2018
- 2018-11-11 CN CN201811335912.9A patent/CN109845642A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007086928A3 (en) * | 2006-01-12 | 2010-08-26 | Cets, Llc | Controlled environment system and method for rapid propagation of seed potato stocks |
| CN103853191A (en) * | 2012-11-30 | 2014-06-11 | 陕西科林能源发展股份有限公司 | Intelligent sunshine directional reflecting device |
| CN103336517A (en) * | 2013-07-02 | 2013-10-02 | 周庆芬 | Intelligent agricultural integrated management system |
| WO2015136926A1 (en) * | 2014-03-14 | 2015-09-17 | パナソニックＩｐマネジメント株式会社 | Plant cultivation apparatus |
| CN206181930U (en) * | 2016-09-09 | 2017-05-24 | 福建虎伯寮生物集团有限公司 | Gold thread lotus intelligence planting system |
| CN106508661B (en) * | 2016-11-01 | 2018-07-24 | 玉林师范学院 | A method of creating roxburgh anoectochilus terminal bud polyploid resource |
| CN106718723A (en) * | 2016-11-24 | 2017-05-31 | 宣威市绿隆农业有限公司 | A kind of nuisanceless high-yield planting method of roxburgh anoectochilus terminal bud |
| CN108094199A (en) * | 2017-12-14 | 2018-06-01 | 泉州市金草生物技术有限公司 | A kind of roxburgh anoectochilus terminal bud cultivating technique |
| CN108684532A (en) * | 2018-08-13 | 2018-10-23 | 儒兰（福建）生物科技有限公司 | A kind of production technology of polyploid roxburgh anoectochilus terminal bud culture medium and polyploid roxburgh anoectochilus terminal bud |
Non-Patent Citations (3)
| Title |
|---|
| QINGER JIANG等: "Research Progress on Rapid Breeding Technology of Anoectochilus", 《MEDICINAL PLANT》 * |
| 江建铭等: "金线莲组培快繁技术研究", 《时珍国医国药》 * |
| 胡颂平等: "《植物细胞组织培养技术》", 31 August 2014, 中国农业大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112493132A (en) * | 2020-12-17 | 2021-03-16 | 刘泽洋 | Method for improving gas microenvironment of plant tissue culture bottle seedlings and special culture medium |
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