CN108401874A - A kind of tobacco cultivation method based on luminous environment - Google Patents
A kind of tobacco cultivation method based on luminous environment Download PDFInfo
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- CN108401874A CN108401874A CN201810362624.6A CN201810362624A CN108401874A CN 108401874 A CN108401874 A CN 108401874A CN 201810362624 A CN201810362624 A CN 201810362624A CN 108401874 A CN108401874 A CN 108401874A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
- A01G31/06—Hydroponic culture on racks or in stacked containers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Developmental Biology & Embryology (AREA)
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Abstract
The present invention relates to a kind of tobacco cultivation methods based on luminous environment, include the following steps：Step（1）, tobacco seed disinfection；Step（2）, tobacco seed culture；Step（3）, transplanting；Step（4）, the culture of dark phase；Step（5）, photo-irradiation treatment；Step（6）, nutrient solution adjust；Step（7）, topping remove wooden fork.The present invention compared to conventional fluorescent spectrum, keep other growth conditions and intensity of illumination it is identical in the case of, tobacco leaf volume increase at least 35%.Present invention covers overall process of the tobacco from kind to receipts in plant factor, and overall process carries out indoors, and tobacco is completely not by the adverse effect of external environment, it can be achieved that annual continuous production.The present invention can make tobacco plant grow up strong and sturdy, Rapid development, the sound prosperity of root system.
Description
Technical field
The present invention relates to a kind of tobacco cultivation methods, and in particular to a kind of tobacco cultivation method based on luminous environment belongs to
Tobacco cultivation field.
Background technology
Tobacco（Nicotiana tabacum）For Solanaceae（Solanaceae）Nicotiana（Nicotiana）It is annual or more
The Important Economic crop of year life.Tobacco belongs to heliophile, but China's tobacco planting is affected by the external environment seriously, and there are light
According to intensity deficiency, the inadequate problem of light application time.Especially in nursery stage, day is mainly carried out in the cultivation of tobacco seedlings in cured tobacco production at present
Wide canopy concentrates nursery, and plastic film and other Insulations make under originally overcast and rainy weather illumination wretched insufficiency in greenhouse.
Tobacco seedlings hypoevolutism is thereby resulted in, nursery stage is elongated, and cost increases, and seedling quality difference has also seriously affected the yield and product of flue-cured tobacco
Matter.
In recent years, with the rise of plant factor, the development of artificial light source needed for plant growth is promoted, you can in room
It is interior, by artificial means, luminous environment is provided for plant.But different light sources, light quality, light intensity to the growth and development of tobacco leaf,
Morphogenesis, internal structure and photosynthesis characteristics etc. have significant impact.
Tobacco seedling and the light filling in growth period mostly use greatly conventional fluorescent as lighting source at present, and spectrum is basis
Human eye designs the selectivity of light, and product design uses " photometric system " appraisement system, the growth of subband non-plant to need, nothing
Method meets plant growth needs, while significant wastage luminous energy.And light emitting diode（LED）It is few, easy with energy conservation and environmental protection, calorific value
In many important features for being different from other electric light sources such as dispersion or combination control, in Plant Tissue Breeding, nursery, growth course
It is middle to provide illumination using LED, regulate and control light quality and light intensity, can not only organization of regulation control culture plant growth and development and form build
At, shorten cultivation cycle, improve quality, and energy consumption can be reduced, reduce cost.
Current many researchers have carried out the research in terms of a large amount of progress tobacco light fillings using LED light, achieve one
Fixed achievement.Such as：CN105165437A discloses a kind of method etc. promoting tobacco seedling using LED plant growth light sources,
But the specific light that above-mentioned patent is developed both for tobacco potting, for the kind of tobacco in plant factor increasingly flourishing at present
It plants, without specific spectral power distribution, the tobacco planting in plant factor can not be instructed.
Invention content
In order to solve the above technical problem, the present invention provides a kind of tobacco cultivation method based on luminous environment, concrete schemes
It is as follows：
A kind of tobacco cultivation method based on luminous environment, includes the following steps：
Step（1）, tobacco seed disinfection；
Step（2）, tobacco seed culture：
By step（1）Tobacco seed be inoculated into MS culture mediums and cultivated, wherein the spectral power distribution of illumination is as follows：
Wavelength is 380-399nm light quantity subnumber≤1%, and the light quantity subnumber that wavelength is 400-499nm is 26-38%, wavelength 500-599nm
Light quantity subnumber be 35-50%, the light quantity subnumber that wavelength is 600-699nm is 15-20%, and wavelength is the light quantum of 700-780nm
Number≤8%；
Step（3）, transplanting：
Tobacco tissue-cultured seedling is taken out from culture bottle, transplanting is on the Planting board of tobacco water planting module after washing and sterilizing；Then will
Planting board is positioned over plantation rooved face, while squeezing into sufficient nutrient solution in planting groove in advance, so that the root of tissue culture tobacco seedling
Touch nutrient solution；It adjusts nutrient solution concentration and keeps lower level in the tobacco seedling phase so that conductivity is on the left sides 0.6mS/cm
It is right；Nutrient solution is replaced primary for 1-2 weeks；
Step（4）, the culture of dark phase：
Dark phase culture is carried out to tissue culture tobacco seedling during dark phase culture to be covered each tobacco seedling with vial；
Step（5）, photo-irradiation treatment：
After dark phase culture, remove vial, photo-irradiation treatment carried out to tobacco seedling, wherein the spectral power distribution of illumination with
Step（2）In spectral power distribution it is identical；
Step（6）, nutrient solution adjust：
Wait for that tobacco tissue culture growth of seedling after 15 days, turns down nutrient solution liquid level and increases the concentration of nutrient solution, make conductivity
It is maintained at 0.65mS/cm or so, to meet tobacco growing needs；
Step（7）, topping remove wooden fork：
In the Tobacco Flowering phase, topping in due course removes wooden fork, and topping has been beaten at twice according to leaves remained, promotes cigarette strain neat, and guarantee falls Huang one
It causes.
Further, step（1）In, the concrete technology of disinfection is：
It takes full vegetable seeds to move on superclean bench, vegetable seeds is impregnated into 30s, aseptic water washing 2 with 75% alcohol
Time, then 10min is sterilized with 1%AgNO3, it is laggard to blot vegetable seeds surface moisture with aseptic filter paper for sterile water soaking and washing 5 times
Row inoculation, wherein sterile water are through autoclaved ultra-pure water.
Further, step（2）In, condition of culture is as follows：
It it is 25 ± 1 DEG C, 30-50 μm of ol/ of intensity of illumination (m2s) in cultivation temperature, light application time is cultivated under conditions of being 16h/d
20d, germination grow up to tobacco tissue culture seedling.
Further, step（2）In, the MS culture mediums are that MS minimal mediums add the agar that 30g/L sucrose adds 4g/L,
The pH value of culture medium is 5.7.
Further, step（2）In, the temperature of nutrient solution height about 3cm, nutrient solution are 19 ± 1 DEG C.
Further, step（4）In, dark phase culture is specially to carry out direct irradiation without using LED light in 2 days.
Further, step（5）In, photo-irradiation treatment is specially to carry out illumination 12h, dark processing to tissue culture tobacco seedling
12h, intensity of illumination are 300 μm of ol/ (m2s).Dark processing refers to being irradiated without using LED light.
Further, step（3）In, nutrient solution height about 3cm；Step（6）In, nutrient solution height about 1cm.
Further, if tobacco water planting module includes several water planting framves, the dried layer planting groove on water planting frame, is set to kind
The nutrient solution tank planted the Planting board on slot and be connected to each layer planting groove inlet and liquid outlet, planting groove are equipped with Planting board,
Planting board surrounding ratio plants groove width, and several implantation holes are arranged on every piece of Planting board, nutrient solution is housed in planting groove, tobacco seedling
Root is contacted across implantation hole with nutrient solution, and nutrient solution tank one end is connected to top layer's planting groove inlet of several water planting framves, separately
One end is connect with lowest level planting groove liquid outlet, and the pipeline being connected to planting groove inlet is equipped with pump, and adjacent planting groove goes out
Liquid mouth is connected to inlet, and every layer of planting groove top is equipped with several LED light and mini-fan；Further include monitoring device, monitoring dress
Set temperature, humidity and the gas concentration lwevel of detection water planting module in real time.
Further, tobacco water planting module further includes going out set on tank gage, liquid outlet and the inlet of each layer planting groove
Valve, tank gage and valve are connect with monitoring device, control liquid level and the flowing of nutrient solution.
Compared with prior art, the present invention has the beneficial effect that：
（1）The present invention provides a kind of cultivation luminous environments of tobacco in plant factor, and the luminous environment is compared to conventional fluorescent light
Spectrum, reduces the low light-wave energy ratio of tobacco growing demand, improves the high light-wave energy ratio of its demand, keep other lifes
Elongate member and intensity of illumination it is identical in the case of, tobacco leaf volume increase at least 35%.
（2）Present invention covers overall process of the tobacco from kind to receipts in plant factor, especially propose in plant factor
Interior tobacco seedling and specific luminous environment at seedling stage, including spectrum and intensity of illumination.And overall process carries out indoors, tobacco is complete
Entirely not by the adverse effect of external environment, it can be achieved that annual continuous production.
（3）The present invention can make tobacco plant grow up strong and sturdy, Rapid development, the sound prosperity of root system.
（4）The water planting module floor space of the present invention is small, space availability ratio is high, using cycling use of water, flow feed flow, use water
Amount is few.The environmental parameters such as internal environment of the present invention such as temperature, humidity, light application time all can artificial adjustment.Overcome field planting
The influences of the natural conditions such as tobacco is illuminated by the light, season, humiture, drought and waterlogging, it can be achieved that tobacco the plantation of more seasons
（5）In the method for the present invention, dark phase culture is first carried out, then carries out conventional sunshine processing, further promotes growth rate, is improved
Tobacco survival rate.
Description of the drawings
Fig. 1 is the structural schematic diagram of the tobacco water planting module of the present invention；
Fig. 2 is the spectral power distribution figure in the periodicity of illumination in embodiment 1.
Specific implementation mode
With reference to embodiment, the present invention is described in further detail.
It will be understood to those of skill in the art that the following example is merely to illustrate the present invention, and it should not be regarded as limiting this hair
Bright range.In the examples where no specific technique or condition is specified, according to technology or condition described in document in the art
Or it is carried out according to product description.
Embodiment 1
As shown in Figure 1, the tobacco water planting module of the present embodiment, if including water planting frame 1,3 layers of planting groove 2 on water planting frame 1,
The Planting board 3 of two pieces of splicings on planting groove 2 and the nutrient solution being connected to 2 inlet 5 of each layer planting groove and liquid outlet 6
Bucket, 2 surrounding of Planting board are covered on the opening of planting groove, 12 implantation holes are arranged on every piece of Planting board than plantation groove width 1cm,
Nutrient solution is housed, tobacco seedling can be fixed in implantation hole 4 in a manner of existing, and the root of tobacco seedling passes through in planting groove 2
Implantation hole 4 is contacted with nutrient solution, and the pipeline being connected to each layer planting groove inlet on nutrient solution tank is equipped with pump, adjacent planting groove
Liquid outlet be connected to inlet, nutrient solution is flowed into first layer planting groove, nutrient solution passes through by inlet by pumping
The liquid outlet of first layer planting groove enters the inlet of the second layer, then flows into third layer inlet from second layer liquid outlet, finally
Nutrient solution tank is flowed back to from third layer liquid outlet, formation circulates, 14 days circulation primaries of the present embodiment.One layer of planting groove topmost
It as first layer, arranges down successively, every layer of height is 70cm, can meet tobacco growing.
Valve can also be arranged in liquid outlet and inlet position.When nutrient solution height reaches requirement or is flowed,
Valve is opened, nutrient solution enters from inlet 5 or flowed out from liquid outlet 6, returns to nutrient solution tank.
The water planting on every layer of 2 top of planting groove is provided with several LED light 8 and mini-fan 7；It is also provided with monitoring device, is supervised
Control device is connect with thermometer, hygrometer and gas concentration lwevel sensor, detects temperature, the humidity and two of water planting module in real time
Carbonoxide concentration sensor.Tank gage can also be arranged to connect with each layer planting groove 2, valve is electric control valve, monitoring device with
Electric control valve, tank gage connection, tank gage feedback information, the opening and closing of control valve are received by monitoring device.
Planting board is ecru PP materials, and the surrounding ratio of thick 5mm, long 960mm, width 580mm, Planting board plant groove width 1cm,
Increase the leakproofness of planting groove and Planting board.A length of 0.9 m of lamp of LED, power 18W, every layer each 24.
The growing temperatures of tobacco water planting module are 25 DEG C, a concentration of 500ppm of humidity 60%%, Carbon Dioxide in Air.
LED light 8 is arranged at the top of every layer frame, and lamp grows 0.9 m, power 18W, every layer each 24.
Tobacco cultivation method based on luminous environment in the plant factor of the present embodiment is based on above-mentioned tobacco water planting module, packet
Include following steps
Step（1）, tobacco seed disinfection：
It takes full vegetable seeds to move on superclean bench, vegetable seeds is impregnated into 30s, aseptic water washing 2 with 75% alcohol
Time, then 10min is sterilized with 1%AgNO3, it is laggard to blot vegetable seeds surface moisture with aseptic filter paper for sterile water soaking and washing 5 times
Row inoculation, wherein sterile water are through autoclaved ultra-pure water.
Step（2）, tobacco seed culture：
By step（1）Tobacco seed be inoculated into MS culture mediums, cultivation temperature be 25 ± 1 DEG C, intensity of illumination 30-50 μ
mol/(m2S), light application time cultivates 20d under conditions of being 16h/d, and germination grows up to tobacco tissue culture seedling；The MS trainings
It is that MS minimal mediums add the agar that 30g/L sucrose adds 4g/L to support base, and the pH value of culture medium is 5.7；In above-mentioned periodicity of illumination
Spectral power distribution is as follows：Wavelength is that 380-399nm light quantity subnumbers account for 0.2%, and wavelength is that the light quantity subnumber of 400-499nm accounts for
33.1%, wavelength is 44.2% shared by the light quantity subnumber of 500-599nm, and the light quantity subnumber proportion that wavelength is 600-699nm is
17.5%, the light quantity subnumber proportion that wavelength is 700-780nm is 5%, specific as shown in Figure 2.
Step（3）, transplanting：
Tobacco tissue culture seedling is taken out from culture bottle, transplanting is on Planting board after washing and sterilizing；Then Planting board is positioned over
Rooved face is planted, while squeezing into sufficient nutrient solution in planting groove in advance；Nutrient solution height about 3cm, so that tissue culture tobacco seedling
Piece-root grafting contact nutrient solution.The temperature of the nutrient solution is 19 ± 1 DEG C of room temperature, and nutrient solution concentration is kept in the tissue culture tobacco seedling phase
Lower level, EC values（Conductivity）In 0.6mS/cm or so.Nutrient solution is replaced primary for 1 week.About 20 points of nutrient solution circulation primary
Clock or so.
Step（4）, the culture of dark phase：
Tissue culture tobacco seedling carries out culture of dark phase in 2 days by a definite date, i.e., carries out direct irradiation without using LED light in 2 days.It is simultaneously anti-
Only tissue culture tobacco seedling moisture loss is covered each tissue culture tobacco seedling 2 days using vial.
Step（5）, photo-irradiation treatment：
After culture of dark phase in 2 days, vial is removed, the photo-irradiation treatment that routinizes is carried out to tobacco seedling.I.e. in the time,
Illumination 12h, the photo-irradiation treatment of dark 12h are carried out to tissue culture tobacco seedling.Intensity of illumination is 300 μm of ol/ (m2s).Wherein, on
State the same step of spectral power distribution in periodicity of illumination（2）.
Step（6）, nutrient solution adjust：
Wait for that tobacco tissue culture growth of seedling after 15 days, turns down nutrient solution liquid level, is maintained at 1cm or so, and increase nutrient solution
Concentration, make EC values（Conductivity）It is maintained at 0.65mS/cm or so, to meet tobacco growing needs.
Step（7）, topping remove wooden fork：
In the Tobacco Flowering phase, topping removes wooden fork in due course.Topping has been beaten at twice according to leaves remained, promotes cigarette strain neat, and guarantee falls Huang one
It causes.
The nutrient solution that the present embodiment uses is the Glan nutrient solution suddenly of tobacco dedicated 1/8：
MgSO4·7H2O, 30.81mg/L；Ca( NO3 )2·4H2O, 221.39mg/L；NaH2PO4·2H2O, 19.5mg/L；
FeSO4·7H2O, 2.78mg/L；Na2- EDTA, 3.72mg/L；MnCl2·4H2O, 0.905mg/L；H3BO3, 1.43mg/L；
ZnSO4·7H2O, 0.11mg/L；CuSO4·5 H2O, 0.04mg/L；( NH4 )6Mo7O24·2H2O, 0.045mg/L；K2SO4,
10.888mg/L。
Embodiment 2
Tobacco cultivation method based on luminous environment in the plant factor of the present embodiment, wherein step（2）Illumination spectral energy
Distribution is as follows：Wavelength is that 380-399nm light quantity subnumbers are 0.8%, and the light quantity subnumber that wavelength is 400-499nm is 30.2%, wavelength
It is 49% for the light quantity subnumber of 500-599nm, the light quantity subnumber that wavelength is 600-699nm is 19%, and wavelength is the light of 700-780nm
Quantum number 1%.
Step（3）In, nutrient solution is replaced primary for 2 weeks.
The growing temperatures of tobacco water planting module are 26 DEG C, a concentration of 400ppm of humidity 65%, Carbon Dioxide in Air.
Remaining is same as Example 1.
Embodiment 3
Tobacco cultivation method based on luminous environment in the plant factor of the present embodiment, wherein step（2）Illumination spectral energy
Distribution is as follows：Wavelength is that 380-399nm light quantity subnumbers are 0.1%, and the light quantity subnumber that wavelength is 400-499nm is 37%, and wavelength is
The light quantity subnumber of 500-599nm is 36.4%, and the light quantity subnumber that wavelength is 600-699nm is 20%, and wavelength is the light of 700-780nm
Quantum number 7%.
Step（3）In, nutrient solution is replaced primary for 10 days.
The growing temperatures of tobacco water planting module are 28 DEG C, a concentration of 500ppm of humidity 70%, Carbon Dioxide in Air.
Remaining is same as Example 1.
As a contrast, we are irradiated using philips-T5 fluorescent lamps simultaneously, remaining is same as Example 1, specifically
The results are shown in Table 1 for Experimental Comparison：
The lower tobacco growing situation comparison of the different light irradiations of table 1
It can be seen that by the comparison of upper table and use the tobacco cultivation side based on luminous environment in a kind of plant factor of the present invention
Method also makes tobacco growing healthy and strong, and the number of blade increases, well developed root system, embodiment 1-3 can increase production 41% compared to fluorescent lamp respectively,
35%、38%。
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (10)
1. a kind of tobacco cultivation method based on luminous environment, it is characterised in that：Include the following steps：
Step（1）, tobacco seed disinfection；
Step（2）, tobacco seed culture：
By step（1）Tobacco seed be inoculated into MS culture mediums and cultivated, wherein the spectral power distribution of illumination is as follows：
Wavelength is 380-399nm light quantity subnumber≤1%, and the light quantity subnumber that wavelength is 400-499nm is 26-38%, wavelength 500-599nm
Light quantity subnumber be 35-50%, the light quantity subnumber that wavelength is 600-699nm is 15-20%, and wavelength is the light quantum of 700-780nm
Number≤8%；
Step（3）, transplanting：
Tobacco tissue-cultured seedling is taken out from culture bottle, transplanting is on the Planting board of tobacco water planting module after washing and sterilizing；Then will
Planting board is positioned over plantation rooved face, while squeezing into sufficient nutrient solution in planting groove in advance, so that the root of tissue culture tobacco seedling
Touch nutrient solution；It adjusts nutrient solution concentration and keeps lower level in the tobacco seedling phase so that conductivity is on the left sides 0.6mS/cm
It is right；Nutrient solution is replaced primary for 1-2 weeks；
Step（4）, the culture of dark phase：
Dark phase culture is carried out to tissue culture tobacco seedling during dark phase culture to be covered each tobacco seedling with vial；
Step（5）, photo-irradiation treatment：
After dark phase culture, remove vial, photo-irradiation treatment carried out to tobacco seedling, wherein the spectral power distribution of illumination with
Step（2）In spectral power distribution it is identical；
Step（6）, nutrient solution adjust：
Wait for that tobacco tissue culture growth of seedling after 15 days, turns down nutrient solution liquid level and increases the concentration of nutrient solution, make conductivity
It is maintained at 0.65mS/cm or so, to meet tobacco growing needs；
Step（7）, topping remove wooden fork：
In the Tobacco Flowering phase, topping in due course removes wooden fork, and topping has been beaten at twice according to leaves remained, promotes cigarette strain neat, and guarantee falls Huang one
It causes.
2. the tobacco cultivation method according to claim 1 based on luminous environment, it is characterised in that：Step（1）In, disinfection
Concrete technology is：
It takes full vegetable seeds to move on superclean bench, vegetable seeds is impregnated into 30s, aseptic water washing 2 with 75% alcohol
Time, then 10min is sterilized with 1%AgNO3, it is laggard to blot vegetable seeds surface moisture with aseptic filter paper for sterile water soaking and washing 5 times
Row inoculation, wherein sterile water are through autoclaved ultra-pure water.
3. the tobacco cultivation method according to claim 1 based on luminous environment, it is characterised in that：Step（2）In, cultivate item
Part is as follows：
It it is 25 ± 1 DEG C, 30-50 μm of ol/ of intensity of illumination (m2s) in cultivation temperature, light application time is cultivated under conditions of being 16h/d
20d, germination grow up to tobacco tissue culture seedling.
4. the tobacco cultivation method according to claim 1 based on luminous environment, it is characterised in that：Step（2）In, the MS
Culture medium is that MS minimal mediums add the agar that 30g/L sucrose adds 4g/L, and the pH value of culture medium is 5.7.
5. the tobacco cultivation method according to claim 1 based on luminous environment, it is characterised in that：Step（2）In, nutrient solution
The temperature of height about 3cm, nutrient solution are 19 ± 1 DEG C.
6. the tobacco cultivation method according to claim 1 based on luminous environment, it is characterised in that：Step（4）In, dark phase training
Support is specially to carry out direct irradiation without using LED light in 2 days.
7. the tobacco cultivation method according to claim 1 based on luminous environment, it is characterised in that：Step（5）In, at illumination
Reason is specially to carry out illumination 12h, dark processing 12h to tissue culture tobacco seedling, and intensity of illumination is 300 μm of ol/ (m2s).
8. the tobacco cultivation method according to claim 1 based on luminous environment, it is characterised in that：Step（3）In, nutrient solution
Height about 3cm；Step（6）In, nutrient solution height about 1cm.
9. the tobacco cultivation method according to claim 1 based on luminous environment, it is characterised in that：Tobacco water planting module includes
If several water planting framves, the dried layer planting groove on water planting frame, the Planting board on planting groove and with each layer planting groove feed liquor
The nutrient solution tank that mouth is connected to liquid outlet, Planting board surrounding are arranged several implantation holes on every piece of Planting board, plant than planting groove width
Nutrient solution is housed, the root of tobacco seedling is contacted across implantation hole with nutrient solution, nutrient solution tank one end and several water planting framves in slot
Top layer's planting groove inlet connection, the other end are connect with lowest level planting groove liquid outlet, the pipe being connected to planting groove inlet
Road is equipped with pump, and the liquid outlet of adjacent planting groove is connected to inlet；Every layer of planting groove top is equipped with several LED light and miniature wind
Fan；Further include monitoring device, monitoring device detects the temperature, humidity and gas concentration lwevel of water planting module in real time.
10. the tobacco cultivation method according to claim 9 based on luminous environment, it is characterised in that：Tobacco water planting module is also
Include the valve gone out set on tank gage, liquid outlet and the inlet of each layer planting groove, tank gage and valve are connect with monitoring device,
Control liquid level and the flowing of nutrient solution.
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| CN201810362624.6A CN108401874A (en) | 2018-04-20 | 2018-04-20 | A kind of tobacco cultivation method based on luminous environment |
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| CN201810362624.6A CN108401874A (en) | 2018-04-20 | 2018-04-20 | A kind of tobacco cultivation method based on luminous environment |
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| CN201810362624.6A Pending CN108401874A (en) | 2018-04-20 | 2018-04-20 | A kind of tobacco cultivation method based on luminous environment |
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| CN108834873A (en) * | 2018-09-12 | 2018-11-20 | 云南中烟工业有限责任公司 | A kind of automation plant water planting system and method |
| CN109156331A (en) * | 2018-09-21 | 2019-01-08 | 山东长兴农业发展有限公司 | A kind of intelligence vegetable aquaculture planting unit |
| CN109275557A (en) * | 2018-09-18 | 2019-01-29 | 云南中烟工业有限责任公司 | A kind of automation tobacco solid floating-seedling device and method |
| EP3767155A4 (en) * | 2019-02-15 | 2021-12-15 | Fujian Sanan Sino-Science Photobiotech Co., Ltd. | Led plant growth lamp light spectrum |
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| CN204949003U (en) * | 2015-06-18 | 2016-01-13 | 爱盛生物科技（上海）有限公司 | Three -dimensional plant soilless culture device |
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| CN108834873A (en) * | 2018-09-12 | 2018-11-20 | 云南中烟工业有限责任公司 | A kind of automation plant water planting system and method |
| CN109275557A (en) * | 2018-09-18 | 2019-01-29 | 云南中烟工业有限责任公司 | A kind of automation tobacco solid floating-seedling device and method |
| CN109156331A (en) * | 2018-09-21 | 2019-01-08 | 山东长兴农业发展有限公司 | A kind of intelligence vegetable aquaculture planting unit |
| CN109156331B (en) * | 2018-09-21 | 2020-11-10 | 山东长兴农业发展有限公司 | Intelligent water planting vegetable planting device |
| EP3767155A4 (en) * | 2019-02-15 | 2021-12-15 | Fujian Sanan Sino-Science Photobiotech Co., Ltd. | Led plant growth lamp light spectrum |
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Application publication date: 20180817 |