CN111357652A - Culture medium for inducing proliferation of cymbidium sinense rhizomes - Google Patents
Culture medium for inducing proliferation of cymbidium sinense rhizomes Download PDFInfo
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- CN111357652A CN111357652A CN202010340536.3A CN202010340536A CN111357652A CN 111357652 A CN111357652 A CN 111357652A CN 202010340536 A CN202010340536 A CN 202010340536A CN 111357652 A CN111357652 A CN 111357652A
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The invention discloses a culture medium for inducing cymbidium sinense rhizome proliferation, which comprises the following components in formula: 1/2MS culture medium, sugar 20-40g/L, coconut juice 200-L, IBA5-15mg/L, 6Ba20-25mg/L, 2, 4-D10-15 mg/L, activated carbon 2g/L, agar 5-10 g/L; the culture medium for the proliferation of the cymbidium floridum prepared by the invention has higher average increment number for the culture of the cymbidium floridum, longer stem length and better growth state of plants, so that the cymbidium floridum has higher quality; and used a culture solution preparation facilities at the in-process of preparation culture medium, the device makes the assembly of nutrient solution need not manual operation completely, has avoided scalding the personnel because of the culture solution is crossed to scald, accomplishes the reinforced back of culture solution, can take out year thing board to directly place new year thing board on the support, and then reduce the erlenmeyer flask and collect the time of putting, accelerated the work efficiency of culture solution preparation.
Description
Technical Field
The invention belongs to the technical field of orchid culture, and particularly relates to a culture medium for inducing cymbidium sinense rhizome proliferation.
Background
The cymbidium sinense, the traditional famous flower in China, is a high-grade orchid with great ornamental value, but because the propagation coefficient is low, the large-scale production is hindered, and the tissue culture is an important way for solving the problem, but the traditional propagation method generally adopts plant division propagation, can be carried out only once in 3-4 years, has slow propagation speed, and is difficult to meet the market demand.
Traditional plant rhizome proliferation culture medium is when carrying out the cymbidium cultivation, and the average multiplication rate of cymbidium is lower, the stem length is shorter, growth speed is slow, and the cymbidium stem of cultivateing is thin little, the leaf color is yellow, and then has influenced the quality of cymbidium to in the preparation process of rhizome proliferation culture medium, owing to need complete manual operation, make the preparation efficiency greatly reduced of culture medium, and because the culture solution is higher in the preparation in-process temperature, easily cause personnel to scald.
Disclosure of Invention
The invention aims to provide a culture medium for inducing proliferation of cymbidium sinense rhizomes.
The technical problems to be solved by the invention are as follows:
traditional culture medium of plant rhizome hyperplasia when carrying out cymbidium rhizome culture, the average number of addendum of cymbidium rhizome is lower, the stem length is shorter, growth rate is slow, the cymbidium rhizome of cultivateing is thin and small, the leaf color is yellow, and then influenced the quality of cymbidium, and in the preparation process of the culture medium of rhizome hyperplasia, owing to need complete manual operation, make the preparation efficiency greatly reduced of culture medium, and because the culture solution is higher in the preparation process, easily cause personnel to scald.
The purpose of the invention can be realized by the following technical scheme:
a culture medium for inducing proliferation of cymbidium sinense rhizomes comprises the following components in parts by weight: the formula comprises the following components: 1/2MS culture medium, sugar 20-40g/L, coconut juice 200-L, IBA5-15mg/L, 6Ba20-25mg/L, 2, 4-D10-15 mg/L, activated carbon 2g/L, agar 5-10 g/L;
the preparation steps of the rooting medium are as follows:
step S1: weighing corresponding components according to the formula;
step S2: adding the components weighed in the step S1 into a culture solution preparation device to prepare a primary culture solution, adding a sodium hydroxide solution into the primary culture solution until the pH value of the primary culture solution reaches 5.3 to prepare a culture solution, and filling the culture solution into a conical flask for later use;
step S3: and (4) placing the conical flask obtained in the step S2 into a sterilization pot, sterilizing for 20-30min at the temperature of 100-110 ℃, and cooling to room temperature to obtain a culture medium for inducing proliferation of cymbidium.
Further, the mass fraction of the sodium hydroxide solution in step S3 is 10 to 15%.
Further, step S2 culture solution preparation facilities, including the working tank, three pillar are evenly connected to the working tank lower extreme outside, three pillar bottom links to each other through the base, the upper end left side of working tank is connected with the inlet pipe, the upper end of inlet pipe is equipped with the apron, the apron passes through the connecting axle and links to each other with the inlet pipe, the upper end outside mid-mounting of working tank has the motor, the axle of motor stretches the end and connects the (mixing) shaft, the inside extension department of stirring axial working tank is equipped with two puddlers, the lower extreme of puddler is equipped with discharging device, discharging device connects the bottom at the working tank, a partial shipment device is connected to the discharging device lower extreme, discharging device passes through flange joint with the partial shipment device, the lower extreme of partial shipment device is equipped with the support, the upper surface of support is equipped with carries the thing board, the cylinder pole.
Further, discharging device includes the discharging pipe, and the discharging pipe is connected in the bottom of working tank, and a circular baffle is fixed through three supporting legs in the upper end of discharging pipe, and the lower extreme of discharging pipe is equipped with flow control valve.
Furthermore, the three supporting legs are arranged at the lower end of the circular partition plate in an equilateral triangle structure, one ends of the supporting legs are welded at the lower end of the circular partition plate, and the other ends of the supporting legs are welded at one end, close to the discharge pipe, of the bottom of the working tank.
Furthermore, the sub-packaging device comprises a sub-packaging pipe, the lower end of the sub-packaging pipe is communicated with a plurality of blanking pipes which are uniformly distributed, and the bottom end of each blanking pipe is in threaded connection with a blanking head.
Further, the side wall of the working tank is provided with a heater.
Further, the lateral wall of support and three pillar laminating mutually, the upper surface of support is equipped with the board groove, the board inslot portion is equipped with the draw-in groove.
Further, the year thing board be circular, the upper surface of carrying the thing board is equipped with a plurality of evenly distributed's year thing groove, carries thing groove and first one-to-one correspondence of unloading, one side of carrying the thing board is fixed with the half-circular arc cardboard, the cardboard cooperatees with the draw-in groove, the lower surface of carrying the thing board is laminated with the bottom surface in board groove mutually.
The invention has the beneficial effects that:
the MS culture medium is added in the process of preparing the culture medium for inducing proliferation of cymbidium sinense rhizomes, the concentration of inorganic salts and ions in the MS culture medium is higher, the MS culture medium is a stable ion balance solution, the content of nitrate is high, the quantity and the proportion of nutrients are proper, the nutrition and physiological requirements of plant cells can be met, sugar can be used as a carbon source substance to provide energy for the plant cells, the osmotic pressure in the culture medium can be better adjusted, the concentration of auxin can be increased by the sugar, the xylem of plants can be formed, coconut juice can provide glucose for the plants, 6Ba belongs to cytokinin, the cell division can be well promoted, the plants can be induced to form new buds, the lateral buds of the plants can be well helped, the top advantages can be broken, the normal flowering of the short-day plants can be promoted, the flowering time of the short-day plants is longer, and the cold resistance of the plants can be improved, 2,4-D promotes or inhibits the growth of certain organs, so that the stem base becomes thick, IBA can promote the growth of the main root of the plant, the germination rate and the survival rate are improved, the active carbon can adsorb harmful secondary metabolites produced by the plant, the rooting of certain plants is facilitated, and good effects on plant morphogenesis and organ formation are achieved; the culture solution preparation device is used in the preparation process, a user can place the conical flask by using a new carrying plate in the process of feeding the conical flask through the arrangement of the carrying plate, the carrying plate can be taken out after feeding is finished, the new carrying plate is directly placed on the support, the time for collecting and placing the conical flask is further reduced, the working efficiency of culture solution preparation is improved, the support is driven by the air cylinder to move upwards, the blanking head is positioned in the opening of the conical flask, the valve in the blanking through hole is opened, the culture solution enters the conical flask from the blanking head, the blanking head is in threaded connection with the blanking pipe, the blanking head can be replaced according to the size of the opening of the conical flask, the culture solution is further prevented from spilling outside the conical flask, and the arrangement of the support and the carrying plate ensures that the assembly of the nutrient solution completely does not need manual operation, avoids scalding of personnel caused by excessive scalding of nutrient solution, and greatly improves the preparation efficiency of the culture medium for inducing proliferation of cymbidium sinense rhizomes.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view showing the construction of a culture solution preparing apparatus according to the present invention;
FIG. 2 is a schematic view showing the structure of a discharging device in the culture solution preparing apparatus according to the present invention;
FIG. 3 is a schematic view showing the structure of a packaging unit in the culture solution preparing apparatus according to the present invention;
FIG. 4 is a plan view of a partitioning device in the culture solution preparing apparatus according to the present invention;
FIG. 5 is a plan view of a carrier plate in the culture solution preparing apparatus according to the present invention;
FIG. 6 is a plan view of a holder in the culture solution preparing apparatus according to the present invention;
FIG. 7 is a side view of a holder in the culture solution preparing apparatus according to the present invention.
In the figure: 1-working tank, 2-pillar, 3-discharging device, 4-support, 5-cylinder rod, 6-cylinder, 7-base, 8-subpackaging device, 9-stirring shaft, 10-stirring rod, 11-motor, 12-feeding pipe, 13-cover plate, 14 connecting shaft, 15-circular partition plate, 16-supporting leg, 17-discharging pipe, 18-flow control valve, 19-flange, 20-subpackaging pipe, 21-discharging pipe, 22-discharging head, 23-carrying plate, 24-carrying groove, 25-clamping plate, 26-plate groove and 27-clamping groove.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A culture medium for inducing proliferation of cymbidium sinense rhizomes comprises the following components in parts by weight: 1/2MS culture medium, sugar 25g/L, coconut juice 240ml/L, IBA 8mg/L, 6Ba 22mg/L, 2, 4-D11 mg/L, activated carbon 2g/L, agar 6 g/L;
the preparation steps of the rooting medium are as follows:
step S1: weighing corresponding components according to the formula;
step S2: adding the components weighed in the step S1 into a culture solution preparation device to prepare a primary culture solution, adding a sodium hydroxide solution into the primary culture solution until the pH value of the primary culture solution reaches 5.3 to prepare a culture solution, and filling the culture solution into a conical flask for later use;
step S3: and (4) putting the conical flask obtained in the step (S2) into a sterilizing pot, sterilizing at 105 ℃ for 28min, and cooling to room temperature to obtain a culture medium for inducing proliferation of cymbidium sinense rhizomes.
Example 2
A culture medium for inducing proliferation of cymbidium sinense rhizomes comprises the following components in parts by weight: 1/2MS culture medium, sugar 24g/L, coconut juice 260ml/L, IBA 9mg/L, 6Ba 23mg/L, 2, 4-D14 mg/L, activated carbon 2g/L, agar 7 g/L;
the preparation steps of the rooting medium are as follows:
step S1: weighing corresponding components according to the formula;
step S2: adding the components weighed in the step S1 into a culture solution preparation device to prepare a primary culture solution, adding a sodium hydroxide solution into the primary culture solution until the pH value of the primary culture solution reaches 5.3 to prepare a culture solution, and filling the culture solution into a conical flask for later use;
step S3: and (4) putting the conical flask obtained in the step (S2) into a sterilizing pot, sterilizing for 21min at the temperature of 106 ℃, and cooling to room temperature to obtain a culture medium for inducing proliferation of cymbidium sinense rhizomes.
Example 3
A culture medium for inducing proliferation of cymbidium sinense rhizomes comprises the following components in parts by weight: 1/2MS culture medium, sugar 35g/L, coconut juice 280ml/L, IBA 10mg/L, 6Ba20 mg/L, 2, 4-D10 mg/L, activated carbon 2g/L, agar 8 g/L;
the preparation steps of the rooting medium are as follows:
step S1: weighing corresponding components according to the formula;
step S2: adding the components weighed in the step S1 into a culture solution preparation device to prepare a primary culture solution, adding a sodium hydroxide solution into the primary culture solution until the pH value of the primary culture solution reaches 5.3 to prepare a culture solution, and filling the culture solution into a conical flask for later use;
step S3: and (4) putting the conical flask obtained in the step (S2) into a sterilizing pot, sterilizing for 23min at the temperature of 110 ℃, and cooling to room temperature to obtain a culture medium for inducing proliferation of cymbidium sinense rhizomes.
Comparative example 1
The comparative example is a culture medium for plant rhizome proliferation commonly found in the market.
The culture media for plant rhizome proliferation prepared in examples 1 to 3 and comparative example 1 were tested, and the test results are shown in table 1 below;
and (3) proliferation of the rhizome: the cymbidium rhizome with the size of 1cm is cultured by the culture medium prepared in the examples 1-3 and the comparative examples 1-3, 20 bottles are cultured in each group, 5 bottles are cultured, the culture parameters such as illumination time, illumination intensity, temperature control and the like are completely consistent, the average proliferation number and the average root length are checked and calculated after 30 days, the average proliferation number is (the number of proliferated explants-the number of inoculated explants)/the number of inoculated explants, and the average stem length is the total length of the stem/total number of stems.
TABLE 1
As can be seen from Table 1 above, the medium for proliferating the rhizome prepared in examples 1-3 has an average proliferation number of 4.9-5.3% for cymbidium culture, while the average proliferation number of comparative example 1 is only 2.5, the average stem length of examples 1-3 is 1.85-1.96cm, while the average stem length of comparative example 1 is 0.98cm, the plants of examples 1-3 have a strong stem, fast growth and normal leaf color, while the plants of comparative example 1 have a thin and small stem, slow growth and yellow leaf color, indicating that the medium for proliferating the rhizome prepared by the present invention has a higher average proliferation number of cymbidium for inducing cymbidium, a longer stem, and a better growth state of the plants, so that the cymbidium quality is higher.
Referring to fig. 1-7, the apparatus for preparing a culture solution according to the above embodiment includes a working tank 1, three pillars 2 are uniformly connected to the outer side of the lower end of the working tank 1, the bottoms of the three pillars 2 are connected to each other through a base 7, a feeding pipe 12 is connected to the left side of the upper end of the working tank 1, a cover plate 13 is disposed on the upper end of the feeding pipe 12, the cover plate 13 is connected to the feeding pipe 12 through a connecting shaft 14, a motor 11 is mounted in the middle of the outer side of the upper end of the working tank 1, a shaft extension end of the motor 11 is connected to a stirring shaft 9, two stirring rods 10 are disposed at the extension portion of the stirring shaft 9 toward the inside of the working tank 1, a discharging device 3 is disposed at the lower end of the stirring rod 10, the discharging device 3 is connected to the bottom of the working tank 1, a sub-packaging device 8 is connected to the lower, the lower end of the support 4 is connected with a cylinder rod 5, the lower end of the cylinder rod 5 is connected with a cylinder 6, and the lower end of the cylinder 6 is fixedly connected to the center of a base 7;
the discharging device 3 comprises a discharging pipe 17, the discharging pipe 17 is connected to the bottom of the working tank 1, a circular baffle plate 15 is fixed at the upper end of the discharging pipe 17 through three supporting legs 16, a flow control valve 18 is arranged at the lower end of the discharging pipe 17, a circular partition plate 15 is arranged to guide the flow direction of liquid, the formation of a vortex is broken, the discharging speed of the liquid is accelerated, and time and labor are saved;
the sub-packaging device 8 comprises a sub-packaging pipe 20, the lower end of the sub-packaging pipe 20 is communicated with a plurality of uniformly distributed blanking pipes 21, and the bottom ends of the blanking pipes 21 are in threaded connection with blanking heads 22.
And the side walls of the working tank 1 are provided with heaters.
The three supporting legs 16 are uniformly arranged at the lower end of the circular partition plate 15 in an equilateral triangle structure, one end of each supporting leg 16 is welded at the lower end of the circular partition plate 15, the other end of each supporting leg 16 is welded at one end, close to the discharge pipe 17, of the bottom of the working tank 1, the circular partition plate 15 is fixed by the three supporting legs 16 in a welding mode, and the circular partition plate 15 can stably bear impact force brought by liquid due to the stability of the triangle without influencing the flow speed of the liquid.
The side wall of the support 4 is attached to the three pillars 2, the upper surface of the support 4 is provided with a plate groove 26, and a clamping groove 27 is formed in the plate groove 26.
Carry thing board 23 be circular, the upper surface that carries thing board 23 is equipped with a plurality of evenly distributed carries thing groove 24, carries thing groove 24 and blanking head 22 one-to-one, one side of carrying thing board 23 is fixed with half-circular arc cardboard 25, cardboard 25 cooperatees with draw-in groove 27, the lower surface that carries thing board 23 laminates with the bottom surface in board groove 26 mutually.
Working steps and working principle:
placing the conical flask in the loading groove 24, placing the loading plate 23 on the support 4, arranging the loading plate 23 to enable a user to use a new loading plate 23 to place the conical flask in the process of feeding the conical flask, taking out the loading plate 23 after feeding is completed, directly placing the new loading plate 23 on the support 4, further reducing the time for collecting and placing the conical flask, and accelerating the working efficiency of culture solution preparation, during preparation, opening the cover plate 13, adding MS culture medium and agar into the working tank 1, closing the cover plate 13, then starting the motor 11, starting the heater, heating and stirring for 10 minutes, closing the motor 11, opening the cover plate 13, adding sugar, coconut juice, IBA, 6Ba, 2,4-D and activated carbon into the working tank 1, starting the motor 11, stirring and heating, driving the stirring shaft 9 to rotate by the motor 11, thereby driving the stirring rod 10 to rotate, stirring materials to ensure that an MS culture medium and agar are melted and mixed firstly, then adding sugar, coconut juice, IBA, 6Ba, 2,4-D and activated carbon, uniformly mixing, stirring, adding a sodium hydroxide solution after uniformly stirring until the pH value of a mixed solution reaches 5.3 to prepare a culture solution, opening the air cylinder 6, driving the support 4 to move upwards by the air cylinder 6, further ensuring that the blanking head 22 is positioned inside the mouth of the conical flask, opening the flow control valve 18 on the discharge pipe 17, controlling the flow rate through the flow control valve 18 to control the amount of the materials, leading the culture solution to enter the conical flask from the blanking head 22, leading the blanking head 22 to be in threaded connection with the blanking pipe 21, leading the blanking head 22 to be replaced according to the size of the mouth of the conical flask, further preventing the culture solution from spilling outside the conical flask, arranging the support 4 and the carrying plate 23, leading the assembly of the nutrient solution to completely without manual operation, avoiding the scalding of personnel due to the excessive scalding of the, greatly improves the preparation efficiency of the culture medium for inducing proliferation of cymbidium sinense rhizomes.
The foregoing is merely exemplary and illustrative of the principles of the present invention and various modifications, additions and substitutions of the specific embodiments described herein may be made by those skilled in the art without departing from the principles of the present invention or exceeding the scope of the claims set forth herein.
Claims (10)
1. A culture medium for inducing proliferation of cymbidium sinense rhizomes, which is characterized in that: the formula comprises the following components: 1/2MS culture medium, sugar 20-40g/L, coconut juice 200-L, IBA5-15mg/L, 6Ba20-25mg/L, 2, 4-D10-15 mg/L, activated carbon 2g/L, agar 5-10 g/L;
the preparation steps of the rooting medium are as follows:
step S1: weighing corresponding components according to the formula;
step S2: adding the components weighed in the step S1 into a culture solution preparation device to prepare a primary culture solution, adding a sodium hydroxide solution into the primary culture solution until the pH value of the primary culture solution reaches 5.3 to prepare a culture solution, and filling the culture solution into a conical flask for later use;
step S3: and (4) placing the conical flask obtained in the step S2 into a sterilization pot, sterilizing for 20-30min at the temperature of 100-110 ℃, and cooling to room temperature to obtain a culture medium for inducing proliferation of cymbidium.
2. The culture medium for inducing proliferation of cymbidium sinense rhizomes, according to claim 1, wherein: the mass fraction of the sodium hydroxide solution in the step S3 is 10-15%.
3. The culture medium for inducing proliferation of cymbidium sinense rhizomes, according to claim 1, wherein: step S2 the culture solution preparation device comprises a working tank (1), three pillars (2) are uniformly connected to the outer side of the lower end of the working tank (1), the bottoms of the three pillars (2) are connected through a base (7), a feeding pipe (12) is connected to the left side of the upper end of the working tank (1), a cover plate (13) is arranged at the upper end of the feeding pipe (12), the cover plate (13) is connected with the feeding pipe (12) through a connecting shaft (14), a motor (11) is installed in the middle of the outer side of the upper end of the working tank (1), a shaft extending end of the motor (11) is connected with a stirring shaft (9), two stirring rods (10) are arranged at the extending position of the stirring shaft (9) towards the inside of the working tank (1), a discharging device (3) is arranged at the lower end of the stirring rods (10), the discharging device (3) is connected to the bottom of the working tank (1), a sub-packaging device (8) is connected to, the lower extreme of partial shipment device (8) is equipped with support (4), and the upper surface of support (4) is equipped with carries thing board (23), and cylinder rod (5) are connected to support (4) lower extreme, and cylinder (6) are connected to cylinder rod (5) lower extreme, and cylinder (6) lower extreme fixed connection is on the center of base (7).
4. The culture medium for inducing proliferation of cymbidium sinense rhizomes as claimed in claim 3, wherein: the discharging device (3) comprises a discharging pipe (17), the discharging pipe (17) is connected to the bottom of the working tank (1), a circular baffle (15) is fixed to the upper end of the discharging pipe (17) through three supporting legs (16), and a flow control valve (18) is arranged at the lower end of the discharging pipe (17).
5. The culture medium for inducing proliferation of cymbidium sinense rhizomes as claimed in claim 4, wherein: the three supporting legs (16) are evenly arranged at the lower end of the circular partition plate (15) in an equilateral triangle structure, one ends of the supporting legs (16) are welded at the lower end of the circular partition plate (15), and the other ends of the supporting legs (16) are welded at one end, close to the discharge pipe (17), of the bottom of the working tank (1).
6. The culture medium for inducing proliferation of cymbidium sinense rhizomes as claimed in claim 3, wherein: the sub-packaging device (8) comprises a sub-packaging pipe (20), the lower end of the sub-packaging pipe (20) is communicated with a plurality of uniformly distributed blanking pipes (21), and the bottom ends of the blanking pipes (21) are in threaded connection with blanking heads (22).
7. The culture medium for inducing proliferation of cymbidium sinense rhizomes as claimed in claim 3, wherein: the side walls of the working tank (1) are provided with heaters.
8. The culture medium for inducing proliferation of cymbidium sinense rhizomes as claimed in claim 3, wherein: the side wall of support (4) laminate mutually with three pillar (2), the upper surface of support (4) is equipped with board groove (26), board groove (26) inside is equipped with draw-in groove (27).
9. The culture medium for inducing proliferation of cymbidium sinense rhizomes as claimed in claim 3, wherein: carry thing board (23) be circular, the upper surface that carries thing board (23) is equipped with a plurality of evenly distributed carries thing groove (24), carries thing groove (24) and blanking head (22) one-to-one, one side of carrying thing board (23) is fixed with half-circular arc cardboard (25), cardboard (25) and draw-in groove (27) cooperate, the lower surface that carries thing board (23) laminates with the bottom surface in board groove (26) mutually.
10. The culture medium for inducing proliferation of cymbidium sinense rhizomes, according to claim 1, wherein: the specific steps of step S2 are as follows:
placing a conical flask in a carrying groove (24), placing a carrying plate (23) on a support (4), during preparation, opening a cover plate (13), adding an MS culture medium and agar into a working tank (1), closing the cover plate (13), then starting a motor (11), starting a heater, heating and stirring for 10 minutes, closing the motor (11), opening the cover plate (13), adding sugar, coconut juice, IBA, 6Ba, 2,4-D and activated carbon into the working tank (1), starting the motor (11), stirring and heating, driving a stirring shaft (9) to rotate by the motor (11), driving a stirring rod (10) to rotate, stirring materials, completing melting and mixing of the MS culture medium and agar, adding the sugar, the coconut juice, IBA, 6Ba, 2,4-D and the activated carbon, uniformly mixing, stirring, adding a sodium hydroxide solution after uniform stirring, and (3) preparing a culture solution when the pH value of the mixed solution reaches 5.3, starting the air cylinder (6), driving the support (4) to move upwards by the air cylinder (6), further enabling the blanking head (22) to be positioned inside the opening of the conical flask, starting the flow control valve (18) on the discharge pipe (17), and enabling the culture solution to enter the conical flask from the blanking head (22).
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116420622A (en) * | 2023-04-24 | 2023-07-14 | 玉林师范学院 | Method for promoting proliferation of cymbidium sinense |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116420622A (en) * | 2023-04-24 | 2023-07-14 | 玉林师范学院 | Method for promoting proliferation of cymbidium sinense |
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Application publication date: 20200703 |