CN111449998A

CN111449998A - Planting extract anti-hair loss hair-growing liquid and preparation method thereof

Info

Publication number: CN111449998A
Application number: CN202010219585.1A
Authority: CN
Inventors: 潘立宁; 黄汉辉; 梁丹; 庞胜
Original assignee: Guangxi Guanshuo Technology Co ltd
Current assignee: Guangxi Guanshuo Technology Co ltd
Priority date: 2020-03-25
Filing date: 2020-03-25
Publication date: 2020-07-28
Anticipated expiration: 2040-03-25
Also published as: CN111449998B

Abstract

The invention discloses a planting extraction anti-hair loss and hair growth liquid and a preparation method thereof, wherein the planting extraction anti-hair loss and hair growth liquid comprises a tea bran fermentation extracting solution and a plant extracting solution, and the mass ratio of the tea bran fermentation extracting solution to the plant extracting solution is 0.8-1.25: 0.7 to 1.25; wherein the tea bran fermentation extract is prepared by the steps of water decoction and reduced pressure concentration of tea bran after the tea bran acts through active yeast; the plant extract is a mixed solution of fleece-flower root extract, Chinese arborvitae twig extract, eclipta alba extract, angelica sinensis extract, mulberry bark extract and liquorice extract. The invention provides a planting extraction hair loss prevention and hair growth solution which comprises tea bran, polygonum multiflorum, cacumen biotae, eclipta alba, angelica sinensis, cortex mori radicis, liquorice and active yeast.

Description

Planting extract anti-hair loss hair-growing liquid and preparation method thereof

Technical Field

The invention belongs to the field of cosmetics, and particularly relates to a planting extraction hair loss prevention and hair growth liquid and a preparation method thereof.

Background

According to the traditional Chinese medicine, the hair is considered to be surplus in blood, the kidney stores essence, the hair is bloomed, the growth of the hair depends on the nourishment of the essence and the blood, the thick and bright hair represents health, a good image can be given to people, the shape and the state of the hair are different, the impression of people is different, and the healthy and beautiful hair enables people to be more confident in interpersonal communication. Similarly, various hair diseases such as alopecia, lack of hair, dandruff, etc. can cause bad psychological symptoms such as self-inferior, anxiety, etc., and serious people even affect social activities, so that care of hair has to be paid attention in daily life.

The hair loss is considered by traditional Chinese medicine due to complexity of hair loss reasons, namely blood heat and wind dryness, blood heat and excess, yin blood consumption and damage, blood tiger wind generation, more yin blood damage, incapability of nourishing hair roots up to the vertex top, hair root drying or hair loss due to deficiency, spleen and stomach damp heat, spleen deficiency and transformation weakness, food fat and thick taste, stomach damage and spleen damage, damp heat and dampness steaming the vertex top, hair roots erosion, hair roots corrosion and hair loss due to stickiness, and head hair loss are also shown by researches, for example, testosterone can be metabolized into Dihydrotestosterone (DHT) under the action of 5 α -reductase (5 α -R), can be combined with receptor proteins in cells to induce specific proteins to generate, and accordingly hair growth is hindered, and accordingly hair loss is caused.

Therefore, the inhibition of the activity of 5 α -reductase is very important, and the hair-growing medicines sold in the market at present have high cost, unsatisfactory treatment effect and general effect of most products.

Disclosure of Invention

This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and the title of the invention of this application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.

The present invention has been made keeping in mind the above and/or other problems occurring in the prior art.

Therefore, the invention aims to overcome the defects in the prior art and provide the planting extraction hair loss prevention and hair growth liquid.

In order to solve the technical problems, the invention provides the following technical scheme: the utility model provides a planting extract hair loss prevention hair tonic which characterized in that: the tea bran fermented extract liquid comprises a tea bran fermented extract liquid and a plant extract liquid, wherein the mass ratio of the tea bran fermented extract liquid to the plant extract liquid is 0.8-1.25: 0.7 to 1.25; wherein,

the tea bran fermented extract is prepared by performing active yeast action on tea bran, decocting with water, and concentrating under reduced pressure, and the preparation method comprises the steps of taking the crushed tea bran, adding the active yeast, uniformly stirring, placing in a container, adding equivalent water, fermenting at 36-38 ℃ for 120-144 hours, taking the fermented product, adding 4-6 times of water by mass, heating to 90-95 ℃, decocting for 1.5-2 hours, filtering to obtain a first decoction liquid, adding 3-4 times of water by mass into the obtained decoction dregs, heating to 85-90 ℃, decocting for 1-1.5 hours, filtering to obtain a first decoction liquid, combining the first decoction liquid and the second decoction liquid, and concentrating under reduced pressure to obtain a tea bran fermented extract;

the plant extract is a mixed solution of a polygonum multiflorum extract, a cacumen biotae extract, an eclipta alba extract, an angelica sinensis extract, a cortex mori radicis extract and a liquorice extract, wherein the mixed mass ratio of the polygonum multiflorum extract, the cacumen biotae extract, the eclipta alba extract, the angelica sinensis extract, the cortex mori radicis extract and the liquorice extract is 8-12: 8-12: 8-12: 4-6: 4-6: 2 to 4.

As a preferable scheme of the plant extract hair loss prevention and hair growth solution, the plant extract hair loss prevention and hair growth solution comprises the following components: adding active yeast into the crushed tea bran, wherein the tea bran is crushed to 40-80 meshes, and the mass ratio of the active yeast to the tea bran is 1-3: 50-60.

As a preferable scheme of the plant extract hair loss prevention and hair growth liquid, the active yeast is Angel yeast, and the viable count of the active yeast is 1 × 10¹⁰CFU/g。

As a preferable scheme of the plant extract hair loss prevention and hair growth solution, the plant extract hair loss prevention and hair growth solution comprises the following components: and combining the first decoction liquid and the second decoction liquid, concentrating under reduced pressure at the temperature of 60-80 ℃ until the specific gravity is 1.25.

As a preferable scheme of the plant extract hair loss prevention and hair growth solution, the plant extract hair loss prevention and hair growth solution comprises the following components: the preparation method of the cacumen biotae extract comprises the steps of cleaning and drying cacumen biotae, crushing the cacumen biotae into 40-80 meshes, soaking the cacumen biotae in 75% alcohol for four times, each time for 3 days, carrying out suction filtration, combining all obtained filtrates, and concentrating at 60 ℃ until the relative density is 1.21 to obtain the cacumen biotae extract; the preparation method of the polygonum multiflorum extract comprises the steps of weighing polygonum multiflorum, cleaning, drying, adding 10-12 weight times of 60% ethanol water solution by volume fraction, heating, refluxing and extracting for 2.5-3 h, filtering, and concentrating the filtrate at 60 ℃ until the relative density is 1.21 to obtain the polygonum multiflorum extract.

As a preferable scheme of the plant extract hair loss prevention and hair growth solution, the plant extract hair loss prevention and hair growth solution comprises the following components: the preparation method of the eclipta alba extract comprises the steps of weighing eclipta alba, cleaning, drying, adding 5-8 times of water, heating to 100 ℃, decocting for 1.5-2 hours, filtering to obtain a first extracting solution, adding 3-5 times of water to the obtained decoction dregs, heating to 100 ℃, decocting for 1 hour, filtering to obtain a second extracting solution, combining the first extracting solution and the second extracting solution, filtering by using a ceramic membrane, carrying out reduced pressure concentration, wherein the concentration temperature is not more than 80 ℃, and concentrating at 60 ℃ to obtain the eclipta alba extract, wherein the specific gravity of the eclipta alba extract is 1.25;

the preparation method of the angelica sinensis extract comprises the steps of weighing angelica sinensis, cleaning, drying, adding 4-6 times of water, heating to 100 ℃, decocting for 1.5-2 hours, filtering to obtain a first extracting solution, adding 3-5 times of water to the obtained decoction dregs, heating to 100 ℃, decocting for 1 hour, filtering to obtain a second extracting solution, combining the first extracting solution and the second extracting solution, filtering by using a ceramic membrane, concentrating under reduced pressure, wherein the concentration temperature is not more than 80 ℃, and concentrating at 60 ℃ to the specific gravity of 1.25 to obtain the eclipta alba extract;

the preparation method of the cortex mori radicis extract comprises the steps of weighing, cleaning and drying the cortex mori radicis, adding 4-6 times of water, heating to 100 ℃, decocting for 1.5-2 hours, filtering to obtain a first extracting solution, adding 3-5 times of water to the obtained decoction dregs, heating to 100 ℃, decocting for 1 hour, filtering to obtain a second extracting solution, combining the first extracting solution and the second extracting solution, filtering by using a ceramic membrane, carrying out reduced pressure concentration, wherein the concentration temperature is not more than 80 ℃, and concentrating at 60 ℃ to obtain the cortex mori radicis extract, wherein the specific gravity is 1.25;

the preparation method of the liquorice peel extract comprises the steps of weighing liquorice peels, cleaning, drying, adding 4-6 times of water, heating to 100 ℃, decocting for 1.5-2 hours, filtering to obtain a first extracting solution, adding 3-5 times of water to the obtained decoction dregs, heating to 100 ℃, decocting for 1 hour, filtering to obtain a second extracting solution, combining the first extracting solution and the second extracting solution, filtering by using a ceramic membrane, carrying out reduced pressure concentration, wherein the concentration temperature is not more than 80 ℃, and concentrating at 60 ℃ to the specific gravity of 1.25 to obtain the liquorice extract.

As a preferable scheme of the plant extract hair loss prevention and hair growth solution, the plant extract hair loss prevention and hair growth solution comprises the following components: the tea bran fermentation extracting solution and the plant extracting solution are mixed according to the mass ratio of 1: 0.8, wherein the mixing mass ratio of the polygonum multiflorum extract, the cacumen biotae extract, the eclipta alba extract, the angelica sinensis extract, the cortex mori extract and the liquorice extract is 10: 8: 12: 6: 4: 4.

as a preferable scheme of the plant extract hair loss prevention and hair growth solution, the plant extract hair loss prevention and hair growth solution comprises the following components: the anti-hair-loss hair-growing liquid further comprises a humectant, a preservative, a thickening agent and deionized water, wherein the thickening agent is xanthan gum, the humectant is 1-10 wt% of 1, 3-butanediol, the preservative is 0.08-0.15 wt% of methyl hydroxybenzoate, a solvent is deionized water, and the mass ratio of the tea bran fermentation extracting solution to the humectant, the preservative, the thickening agent and the deionized water is 0.8-1.25: 0.2-0.4: 0.2-0.6: 0.1-0.2: 10 to 20. .

The invention further aims to overcome the defects in the prior art and provide a preparation method of the planting extraction hair loss prevention hair growth liquid.

In order to solve the technical problems, the invention provides the following technical scheme: a method for preparing plant extract hair loss prevention and hair growth liquid comprises,

preparing a tea bran fermentation extracting solution: adding active yeast into crushed tea bran, uniformly stirring, placing into a container, adding equal amount of water, fermenting for 120-144 h at 36-38 ℃, adding 4-6 times of water by mass into a fermented product, heating to 90-95 ℃, decocting for 1.5-2 h, filtering to obtain a first decoction liquid, adding 3-4 times of water by mass into obtained decoction dregs, heating to 85-90 ℃, decocting for 1-1.5 h, filtering to obtain a first decoction liquid, combining the first decoction liquid and a second decoction liquid, and concentrating under reduced pressure to obtain a tea bran fermentation extract;

taking a polygonum multiflorum extract, a cacumen biotae extract, an eclipta alba extract, an angelica sinensis extract, a cortex mori extract and a liquorice extract according to a mass ratio of 8-12: 8-12: 8-12: 4-6: 4-6: 2-4, mixing to obtain a plant extracting solution;

mixing a tea bran fermentation extract and a plant extract according to a mass ratio of 0.8-1.25: 0.7-1.25, uniformly stirring, adding a humectant, a preservative, a thickening agent and a solvent, and uniformly stirring to obtain the plant extract anti-hair loss and hair growth liquid; the thickening agent is xanthan gum, the humectant is 1-10 wt% of 1, 3-butanediol, the preservative is 0.08-0.15 wt% of methyl hydroxybenzoate, the solvent is deionized water, and the mass ratio of the tea bran fermentation extract to the humectant, the preservative, the thickening agent and the deionized water is 0.8-1.25: 0.2-0.4: 0.2-0.6: 0.1-0.2: 10 to 20.

The invention has the beneficial effects that:

(1) the invention provides a planting extraction hair loss prevention and hair growth solution which comprises tea bran, polygonum multiflorum, cacumen biotae, eclipta alba, angelica sinensis, cortex mori radicis, liquorice and active yeast. The tea bran and the active yeast are utilized to convert macromolecular nutrient substances into absorbable micromolecular nutrient substances, so that harmful ingredients are degraded, and meanwhile, the active ingredients in the tea bran can play a role, and the prepared plant extract hair loss prevention and hair growth solution has strong active ingredients and has the effects of removing dandruff, relieving itching, blackening and brightening the hair, preventing hair loss and growing the hair.

(2) The invention provides a planting extraction hair loss prevention and hair growth liquid, which can change the blood circulation of the head, activate hair follicles and realize the hair restoration effect by the action of tea bran extract and active ingredients in platycladus orientalis leaves and the mutual synergistic action of the active ingredients in polygonum multiflorum, eclipta alba, angelica, white mulberry root-bark and liquorice extract, and the mutual synergistic action of the plant extract ingredients has very strong 5 α -reductase activity inhibition effect.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, specific embodiments thereof are described in detail below with reference to examples of the specification.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.

Furthermore, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.

The preparation method of the cacumen biotae extract comprises the following steps: cleaning folium Platycladi, drying, pulverizing to 40 mesh, soaking in 75% ethanol for four times (each for 3 days), vacuum filtering, mixing all filtrates, and concentrating at 60 deg.C to relative density of 1.21 to obtain folium Platycladi extract.

The preparation method of the polygonum multiflorum extract comprises the following steps: weighing Polygonum multiflorum, cleaning, drying, pulverizing to 80 meshes, adding 10 times by weight of 60% ethanol aqueous solution, heating and refluxing for 3h, filtering, and concentrating the filtrate at 60 ℃ until the relative density is 1.21 to obtain the Polygonum multiflorum extract.

The preparation method of the eclipta alba extract comprises the following steps: weighing eclipta alba, cleaning, drying, adding 8 times of water, heating to 100 ℃, decocting for 2h, filtering to obtain a first extracting solution, adding 3 times of water into obtained decoction dregs, heating to 100 ℃, decocting for 1h, filtering to obtain a second extracting solution, combining the first and second extracting solutions, filtering by using a ceramic membrane, carrying out reduced pressure concentration, and concentrating at 60 ℃ to obtain the eclipta alba extract, wherein the specific gravity of the eclipta alba extract is 1.25.

The preparation method of the angelica extract comprises the following steps: weighing radix Angelicae sinensis, cleaning, drying, adding 6 times of water, heating to 100 deg.C, decocting for 1.5 hr, filtering to obtain first extractive solution, adding 5 times of water to the residue, heating to 100 deg.C, decocting for 1 hr, filtering to obtain second extractive solution, mixing the first and second extractive solutions, filtering with ceramic membrane, concentrating under reduced pressure, and concentrating at 60 deg.C to specific gravity of 1.25 to obtain radix Angelicae sinensis extract.

The preparation method of the cortex mori extract comprises the following steps: weighing cortex Mori, cleaning, drying, adding 4 times of water, heating to 100 deg.C, decocting for 2 hr, filtering to obtain first extractive solution, adding 3 times of water to the residue, heating to 100 deg.C, decocting for 1 hr, filtering to obtain second extractive solution, mixing the first and second extractive solutions, filtering with ceramic membrane, concentrating under reduced pressure, and concentrating at 60 deg.C to specific gravity of 1.25 to obtain cortex Mori extract;

the preparation method of the licorice extract comprises the following steps: weighing liquorice peel, cleaning, drying, adding 5 times of water, heating to 100 ℃, decocting for 2h, filtering to obtain a first extracting solution, adding 3 times of water into the obtained decoction dregs, heating to 100 ℃, decocting for 1h, filtering to obtain a second extracting solution, combining the first and second extracting solutions, filtering by a ceramic membrane, carrying out reduced pressure concentration, and concentrating at 60 ℃ to obtain the liquorice extract, wherein the specific gravity of the liquorice extract is 1.25.

Example 1

The embodiment provides a preparation method of a planting extraction hair loss prevention and hair growth liquid, which comprises the following steps:

(1) preparing a tea bran fermentation extracting solution: taking crushed tea bran (the tea bran is crushed to 80 meshes), adding active yeast (the mass ratio of the active yeast to the tea bran is 3: 50), uniformly stirring, placing in a container, adding equal amount of water, fermenting at 38 ℃ for 120h, taking a fermented product, adding 6 times of water by mass, heating to 90 ℃, decocting for 2h, filtering to obtain a first decoction liquid, adding 4 times of water by mass into obtained decoction dregs, heating to 85 ℃, decocting for 1h, filtering to obtain a first decoction liquid, merging the first decoction liquid and a second decoction liquid, concentrating under reduced pressure (the concentration temperature is 60 ℃, and concentrating until the specific gravity is 1.25) to obtain a tea bran fermentation extract;

(2) taking a polygonum multiflorum extract, a cacumen biotae extract, an eclipta alba extract, an angelica sinensis extract, a cortex mori extract and a liquorice extract according to a mass ratio of 10: 12: 8: 6: 4: 4 mixing to obtain plant extract;

(3) mixing a tea bran fermentation extracting solution and a plant extracting solution according to the mass ratio of 1.25: 1, mixing, uniformly stirring, adding a humectant, a preservative, a thickening agent and a solvent, and uniformly stirring to obtain the plant extract anti-hair loss and hair growth liquid; the thickening agent is xanthan gum, the humectant is 1-10 wt% of 1, 3-butanediol, the preservative is 0.08-0.15 wt% of methyl hydroxybenzoate, the solvent is deionized water, and the mass ratio of the tea bran fermentation extract to the humectant, the preservative, the thickening agent and the deionized water is 1.25: 0.2: 0.4: 0.1: 10.

example 2

(1) preparing a tea bran fermentation extracting solution: taking crushed tea bran (the tea bran is crushed to 80 meshes), adding active yeast (the mass ratio of the active yeast to the tea bran is 1: 50), uniformly stirring, placing in a container, adding equal amount of water, fermenting at 36 ℃ for 144h, taking a fermented product, adding 4 times of water by mass, heating to 90 ℃, decocting for 2h, filtering to obtain a first decoction liquid, adding 4 times of water by mass into obtained decoction dregs, heating to 85 ℃, decocting for 1h, filtering to obtain a first decoction liquid, merging the first decoction liquid and a second decoction liquid, concentrating under reduced pressure (the concentration temperature is 60 ℃, and concentrating until the specific gravity is 1.25) to obtain a tea bran fermentation extract;

(2) taking a polygonum multiflorum extract, a cacumen biotae extract, an eclipta alba extract, an angelica sinensis extract, a cortex mori extract and a liquorice extract according to a mass ratio of 8: 8: 12: 6: 6: 4 mixing to obtain plant extract;

(3) mixing a tea bran fermentation extracting solution and a plant extracting solution according to the mass ratio of 1: 1.25, uniformly stirring, adding a humectant, a preservative, a thickening agent and a solvent, and uniformly stirring to obtain the plant extract anti-hair loss and hair growth liquid; the thickening agent is xanthan gum, the humectant is 1-10 wt% of 1, 3-butanediol, the preservative is 0.08-0.15 wt% of methyl hydroxybenzoate, the solvent is deionized water, and the mass ratio of the tea bran fermentation extract to the humectant, the preservative, the thickening agent and the deionized water is 1.25: 0.2: 0.4: 0.1: 10.

example 3

(1) preparing a tea bran fermentation extracting solution: taking crushed tea bran (the tea bran is crushed to 40 meshes), adding active yeast (the mass ratio of the active yeast to the tea bran is 3: 50), uniformly stirring, placing in a container, adding equal amount of water, fermenting at 38 ℃ for 144h, taking a fermented product, adding 6 times of water by mass, heating to 90 ℃, decocting for 1.5h, filtering to obtain a first decoction liquid, adding 3-4 times of water by mass into obtained dregs, heating to 90 ℃, decocting for 1.5h, filtering to obtain a first decoction liquid, merging the first and second decoction liquids, concentrating under reduced pressure (the concentration temperature is 80 ℃, and concentrating until the specific gravity is 1.25) to obtain a tea bran fermentation extract;

(2) taking a polygonum multiflorum extract, a cacumen biotae extract, an eclipta alba extract, an angelica sinensis extract, a cortex mori extract and a liquorice extract according to a mass ratio of 8: 12: 8: 6: 6: 4 mixing to obtain plant extract;

(3) mixing the tea bran fermentation extract and the plant extract according to the mass ratio of 0.8: 0.7, uniformly stirring, adding a humectant, a preservative, a thickening agent and a solvent, and uniformly stirring to obtain the plant extract anti-hair loss and hair growth liquid; the thickening agent is xanthan gum, the humectant is 1-10 wt% of 1, 3-butanediol, the preservative is 0.08-0.15 wt% of methyl hydroxybenzoate, the solvent is deionized water, and the mass ratio of the tea bran fermentation extract to the humectant, the preservative, the thickening agent and the deionized water is 1.25: 0.4: 0.6: 0.2: 20.

example 4

(1) preparing a tea bran fermentation extracting solution: taking the crushed tea bran (the tea bran is crushed to 80 meshes), adding 6 times of water by mass, heating to 95 ℃, decocting for 2 hours, filtering to obtain a first decoction liquid, adding 4 times of water by mass into the obtained decoction dregs, heating to 90 ℃, decocting for 1.5 hours, filtering to obtain a first decoction liquid, combining the first decoction liquid and the second decoction liquid, and concentrating under reduced pressure (the concentration temperature is 60 ℃, and the concentration is carried out until the specific gravity is 1.25) to obtain a tea bran fermentation extract;

evaluation of the effects: selecting 150 subjects with scalp oil, alopecia areata and baldness easily appearing in 20-50 years old as subjects; 78 men and 72 women; 150 volunteers were divided into 5 groups of 30 persons, the first, second, third and fourth groups used the plant extract hair-loss preventing and hair-growing liquid prepared in examples 1, 2, 3 and 4, respectively, and the fifth group was a hair-loss preventing and hair-growing liquid sample (commercially available sample) which is sold well in the market at present, and the use method was: the hair restorer sample is applied to scalp for 2 months in the morning and evening.

The evaluation standard of the curative effect is as follows: 1) the effect is shown: stopping alopecia, and growing black hair again in hairless areas; 2) the method has the following advantages: the alopecia is obviously improved, and black hair grows on partial hairless areas; 3) and (4) invalidation: the hair loss is not improved and alleviated, and no new hair is grown on the hairless part. The results are shown in Table 1.

TABLE 1

Example 5

The extracts of examples 1 to 4 and 10g of the anti-hair loss and hair growth promoting liquid which is sold in the market at present are added with 23.5g of 1, 3-butanediol and 35.5g of distilled water to obtain the standby products of different test groups (1 st group, 2 nd group, 3 rd group, 4 th group and 5 th group respectively).

(1) A test mouse with the weight of 15-20g is taken, under ether light anesthesia, the hair on the back of the mouse is pulled out by using a mixture of rosin and paraffin, the area is about 3cm × 4cm, and the test mouse is divided into test groups (1 st group, 2 nd group, 3 rd group, 4 th group and 5 th group, 10 animals in each group), a normal control group (6 th group) and a solvent group (1, 3-butanediol group-7 th group).

(2) On the next day after depilation, uniformly smearing each test group of standby products on the skin of the depilation region of the back of each group of mice respectively, wherein the dosage of each test group is 10ml/kg, the control group is given with the same volume of physiological saline, the solvent group is given with the same volume of 1, 3-butanediol, 1 time per day for 10 consecutive days, and the hair growth condition of each group of mice is observed after the application of the drugs for 10 days and is graded according to the hair growth condition of the mice.

And (4) grading standard: no growth, the skin of the depilated area is flesh: 0 minute; the skin in the depilatory area is grey: 1 minute; the skin in the depilated area is black: 2 min; the depilated area had a dark skin color and a few hairs were growing: and 3 minutes.

And (3) taking the skin of the depilated area on the back of the mouse at the end of the test, fixing the skin by formalin, making pathological sections, and observing the growth condition of hair follicles. The effect on hair growth in normal mice is shown in table 2.

TABLE 2

	Fractional administration 10 days after administration	Hair follicle number/visual field
			Group 1	2.7±0.3	182.2±6.4
Group 2	2.4±0.2	162.4±5.2
			Group 3	2.3±0.1	156.5±6.2
Group 4	2.0±0.3	141.4±5.6
			Group 5	1.8±0.2	138.8±3.2
Group 6	1.4±0.1	96.7±5.8
			Group 7	1.2±0.1	92.1±5.2

Example 6

Evaluation test of Effect of inhibiting 5 α -reductase Activity:

group 1 blank test group 5 α -reductase, dependent reduction coenzyme (NADPH), Tris buffer solution;

group 2 blank control group with testosterone, 5 α -reductase, dependent reduction coenzyme (NADPH), Tris buffer solution of series concentration;

the finasteride group is finasteride solution, testosterone with series concentration, 5 α -reductase, dependent reduction coenzyme (NADPH) and Tris buffer solution;

test group 4 test group was a plant extract composition (the content of each plant extract in the extract composition was the same as that of each plant extract in example 1), testosterone, 5 α -reductase, dependent reducing coenzyme (NADPH), Tris buffer solution in series concentration;

in groups 1 to 4, the same reagent was used in the same amount.

And (3) continuously incubating the groups 1-1 at 37 ℃, continuously measuring the time change curve of the characteristic absorption wavelength OD value of the NADPH at 340nm by using an ultraviolet grating spectrophotometer, and calculating the enzyme activity. The results of parallel 5 groups were averaged and are shown in Table 3.

TABLE 3

As can be seen from Table 3, the plant extract hair loss prevention and hair growth promoting liquid of the invention has an inhibition effect on α -reductase activity.

Example 7

(1) Under the preparation process conditions of example 1, the influence of the formulas of the different plant extracts on the hair loss preventing and growing effect of the plant extract hair loss preventing and growing liquid is examined, and the results are shown in table 3.

TABLE 4

	Test 1	Test 2	Test 3	Test 4	Test 5
						Polygonum multiflorum extract	0	0	10	10	10
Cacumen Platycladi extract	0	0	0	12	12
						Ecliptae herba extract	8	0	8	8	0
Angelica sinensis extract	6	6	6	0	6
						Cortex Mori extract	4	4	4	0	0
Glycyrrhiza extract	4	4	4	4	0
						Total effective rate (%)	78.24	72.13	79.12	84.2	83.2

As can be seen from table 4, the tea bran extract and the active ingredients in the cacumen biotae are combined with the active ingredients in the extracts of polygonum multiflorum, eclipta alba, angelica, cortex mori radicis and liquorice, and the active ingredients of the extracts of various plants are mutually synergistic, so that the effects of changing head blood circulation, activating hair follicles and repairing hairs can be realized.

Tea bran, also called tea seed cake, the granule is purple brown, is the residue cake after oil extraction of tea seed, the effective component is saponin. The quality standard of the tea seed cake is as follows: the tea saponin contains 12-18% of tea saponin, less than 2% of residual oil, 12-16% of protein, 30-50% of starch and saccharide, 10-12% of fiber, less than 12% of water and less than 2% of impurity. The tea seed cake contains tea saponin, protein, natural tea oil and the like, has the effects of stopping dandruff, relieving itching, removing oil, sterilizing and repairing damaged hair quality after long-term use, and also has obvious effects of blackening and growing hair, thus being one of the best natural cleaning agents;

active yeast, active dry yeast is a dry yeast product which is prepared by squeezing, drying and dehydrating specially cultured fresh yeast and still keeps strong fermentation capacity. Squeezing the squeezed yeast into strips or pellets, and continuously drying with low-humidity circulating air via fluidized bed to reach final fermentation water content of about 8% and maintain the fermentation capacity of the yeast. The tea bran is used for fermentation, and can be converted into absorbable small molecular substances to degrade harmful components;

polygonum multiflorum (famous name: Fallopia multiflora (Thunb.) Harald.), polygonum multiflorum, glaucescent fissistigma root, tuber fleeceflower stem and the like. Is perennial winding liana of Polygonum of Polygonaceae, with thick root tuber, oblong shape, and dark brown color. The root tuber contains anthraquinone compounds, mainly emodin, chrysophanol, physcion, rhein, and chrysophanol anthrone. Has effects of blackening and moistening hair after washing hair;

cacumen Platycladi, is the dry branch tip and leaf of Franco of Cupressaceae plant Platycladus orientalis P L atycladus orientalis (L.), and the dry branch tip and leaf of Franco of Cupressaceae plant Platycladus orientalis P L atycladus orientalis (L.), is cold in nature, bitter and astringent in taste, and the main components are volatile oil, flavone, tannin, etc. the cacumen Platycladi has effects of cooling blood, stopping bleeding, growing and blackening hair;

eclipta alba, dry aerial part of ecliptaproata L of ecliptastra prostrata of Compositae, sweet, sour and cold, contains chemical components such as volatile oil, tannin, saponin, eclipta prostrata and vitamin A, and has good curative effects on dizziness, odontoseisis, soreness and pain of waist and back, flaccidity and softness of lower limbs caused by liver-kidney yin deficiency and various bleeding syndromes caused by blood heat;

angelica sinensis, a dried root of Angelica sinensis (Oliv.) Diels of Umbelliferae, is sweet and pungent in flavor and warm in nature. Comprises ligustilide, n-butyroyllactone, ferulic acid, nicotinic acid, sucrose, various amino acids, and sesquiterpene compounds. Has the functions of enriching blood, promoting blood circulation, regulating menstruation, relieving pain, moistening intestine and relaxing bowels. After shampooing, the shampoo has the effects of softening, smoothing and preventing alopecia;

cortex Mori is dry root bark of Morus alba L.of Moraceae of dicotyledonous plant, with its taste sweet and cold, and is mainly benzofuran compounds, stilbene compounds, flavonoids, phenylpropanoids, triterpenes, alkaloids, etc. Has various modern pharmacological activities such as blood pressure reduction, anti-inflammation, anticancer and the like. After shampooing, the hair is protected from alopecia;

the liquorice is dried root and rhizome of liquorice, Glycyrrhiza uralensis Fisch, Glycyrrhiza inflata Bat, or Glycyrrhiza glabra Glabra L, which are leguminous plants, has sweet taste and even nature, and has the effects of tonifying spleen and supplementing qi, clearing heat and detoxicating, eliminating phlegm and stopping cough, relieving spasm and pain, and harmonizing all drugs through the effects of the tea bran extract and the active ingredients in the platycladus orientalis leaves, and the active ingredients in polygonum multiflorum, eclipta alba, angelica, white mulberry root bark and liquorice extract are combined, so that the synergistic effect of the active ingredients of various plant extracts is realized, the blood circulation of the head can be changed, the hair follicle can be activated, and the hair restoration effect is realized.

(2) The plant extracts prepared in test 1 and test 2 of this example were evaluated for their effect of inhibiting 5 α -reductase activity according to the test method for evaluating the effect of inhibiting 5 α -reductase activity of example 6, and the results are shown in Table 5.

Test group 5 test group was a plant extract composition (the content of each plant extract in the extract composition was the same as that of each plant extract in test 1), testosterone of series concentration, 5 α -reductase, dependent reducing coenzyme (NADPH), Tris buffer solution;

test group 6 test group was a plant extract composition (the content of each plant extract in the extract composition was the same as that of each plant extract in test 2), testosterone of series concentration, 5 α -reductase, dependent reducing coenzyme (NADPH), Tris buffer solution;

in groups 1 to 6, the same reagent was used in the same amount.

TABLE 5

As can be seen from Table 5, the tea bran extract, the active ingredients in the cacumen Platycladi, the active ingredients in the polygonum multiflorum and the active ingredients in the eclipta alba have a synergistic effect on the inhibition of α -reductase activity.

The invention provides a planting extraction hair loss prevention and hair growth liquid, which can change the blood circulation of the head, activate hair follicles and realize the hair restoration effect by the action of tea bran extract and active ingredients in platycladus orientalis leaves and the mutual synergistic action of the active ingredients in polygonum multiflorum, eclipta alba, angelica, white mulberry root-bark and liquorice extract, and the mutual synergistic action of the active ingredients in various plant extracts, and has very strong effect of inhibiting the activity of 5 α -reductase

The invention provides a planting extraction hair loss prevention and hair growth solution which comprises tea bran, polygonum multiflorum, cacumen biotae, eclipta alba, angelica sinensis, cortex mori radicis, liquorice and active yeast. The tea bran and the active yeast are utilized to convert macromolecular nutrient substances into absorbable micromolecular nutrient substances, so that harmful ingredients are degraded, and meanwhile, the active ingredients in the tea bran can play a role, and the prepared plant extract hair loss prevention and hair growth solution has strong active ingredients and has the effects of removing dandruff, relieving itching, blackening and brightening the hair, preventing hair loss and growing the hair.

It should be noted that the above-mentioned embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.

Claims

1. The utility model provides a planting extract hair loss prevention hair tonic which characterized in that: the tea bran fermented extract liquid comprises a tea bran fermented extract liquid and a plant extract liquid, wherein the mass ratio of the tea bran fermented extract liquid to the plant extract liquid is 0.8-1.25: 0.7 to 1.25; wherein,

2. The plant extract hair loss prevention and hair growth solution according to claim 1, wherein: adding active yeast into the crushed tea bran, wherein the tea bran is crushed to 40-80 meshes, and the mass ratio of the active yeast to the tea bran is 1-3: 50-60.

3. The plant extract hair loss prevention and hair growth liquid as claimed in claim 2, wherein the active yeast is Angel yeast with viable count of 1 × 10¹⁰CFU/g。

4. The plant extract hair loss prevention and hair growth solution according to claim 1, wherein: and combining the first decoction liquid and the second decoction liquid, concentrating under reduced pressure at the temperature of 60-80 ℃ until the specific gravity is 1.25.

5. The plant extract hair loss prevention and hair growth solution according to claim 1, wherein: the preparation method of the cacumen biotae extract comprises the steps of cleaning and drying cacumen biotae, crushing the cacumen biotae into 40-80 meshes, soaking the cacumen biotae in 75% alcohol for four times, each time for 3 days, carrying out suction filtration, combining all obtained filtrates, and concentrating at 60 ℃ until the relative density is 1.21 to obtain the cacumen biotae extract;

the preparation method of the polygonum multiflorum extract comprises the steps of weighing polygonum multiflorum, cleaning, drying, adding 10-12 weight times of 60% ethanol water solution by volume fraction, heating, refluxing and extracting for 2.5-3 h, filtering, and concentrating the filtrate at 60 ℃ until the relative density is 1.21 to obtain the polygonum multiflorum extract.

6. The plant extract hair loss prevention and hair growth solution according to claim 1, wherein: the preparation method of the eclipta alba extract comprises the steps of weighing eclipta alba, cleaning, drying, adding 5-8 times of water, heating to 100 ℃, decocting for 1.5-2 hours, filtering to obtain a first extracting solution, adding 3-5 times of water to the obtained decoction dregs, heating to 100 ℃, decocting for 1 hour, filtering to obtain a second extracting solution, combining the first extracting solution and the second extracting solution, filtering by using a ceramic membrane, carrying out reduced pressure concentration, wherein the concentration temperature is not more than 80 ℃, and concentrating at 60 ℃ to obtain the eclipta alba extract, wherein the specific gravity of the eclipta alba extract is 1.25;

7. The plant extract hair loss prevention and hair growth solution according to claim 1, wherein: the tea bran fermentation extracting solution and the plant extracting solution are mixed according to the mass ratio of 1: 0.8, wherein the mixing mass ratio of the polygonum multiflorum extract, the cacumen biotae extract, the eclipta alba extract, the angelica sinensis extract, the cortex mori extract and the liquorice extract is 10: 8: 12: 6: 4: 4.

8. the plant extract hair loss prevention and hair growth solution according to claim 1, wherein: the anti-hair-loss hair-growing liquid further comprises a humectant, a preservative, a thickening agent and deionized water, wherein the thickening agent is xanthan gum, the humectant is 1-10 wt% of 1, 3-butanediol, the preservative is 0.08-0.15 wt% of methyl hydroxybenzoate, a solvent is deionized water, and the mass ratio of the tea bran fermentation extracting solution to the humectant, the preservative, the thickening agent and the deionized water is 0.8-1.25: 0.2-0.4: 0.2-0.6: 0.1-0.2: 10 to 20.

9. A method for preparing the plant extract hair loss prevention and hair growth liquid according to any one of claims 1 to 8, wherein the method comprises the following steps: comprises the steps of (a) preparing a mixture of a plurality of raw materials,