CN111449074A - Bactericidal composition containing myclobutanil and fludioxonil and application of bactericidal composition to prevention and treatment of corn macule disease - Google Patents
Bactericidal composition containing myclobutanil and fludioxonil and application of bactericidal composition to prevention and treatment of corn macule disease Download PDFInfo
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- CN111449074A CN111449074A CN202010079417.7A CN202010079417A CN111449074A CN 111449074 A CN111449074 A CN 111449074A CN 202010079417 A CN202010079417 A CN 202010079417A CN 111449074 A CN111449074 A CN 111449074A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
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Abstract
The invention discloses a mixed medicament and application thereof in preventing and treating corn northern leaf blight, belonging to the technical field of pesticides. The mixed medicament takes myclobutanil and fludioxonil as active ingredients, and the mass ratio of the two active ingredients is 9: 1 or 1: 9. According to the invention, the bactericides with different action mechanisms are mixed to obtain the novel agent for preventing and treating the corn southern leaf blight, so that the generation of field drug resistance is favorably delayed, the prevention and treatment effect can be improved, the dosage and the use cost are reduced, and the novel agent has potential social, economic and ecological benefits.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition containing myclobutanil and fludioxonil and application thereof in preventing and treating corn septoria.
Background
Fresh corn is an important economic crop in China. Corn northern leaf blight caused by infection of corn leaves by helminthosporium hirsutum (Bipolaris maydis) is a worldwide corn fungal disease. In recent years, with the development of the planting industrialization of fresh corn, the corn leaf spot is increasingly serious in each fresh corn main production area in China. Corn southern leaf blight can occur throughout the corn growth period, and is most beneficial to disease outbreak especially under warm and humid conditions after corn plants are emasculated. The yield of common susceptible varieties is reduced by more than 10 percent, the yield is reduced by 20 to 80 percent in severe years, and even seeds are destroyed and harvested. The disease has the characteristics of universal occurrence, high spreading and expanding speed and the like, and is developed into the most important disease in corn production in China. Chemical control is still an important method for controlling corn northern leaf blight in China. However, the long-term single use of the bactericide for preventing and treating the corn southern leaf blight easily causes the generation of drug resistance of pathogenic bacteria, reduces the prevention and treatment effect and increases the medication cost. Therefore, screening of novel, efficient and low-toxicity bactericide and development of compound medicament thereof are the problems which need to be solved urgently for preventing and controlling the corn small spot.
Myclobutanil [ chinese name: 1- (4-chlorophenyl) -2- (1H-1, 2, 4-triazole-1-methyl) hexanenitrile or 2- (4-chlorophenyl) -2- (1H, 1, 2, 4-triazole-1-methyl) hexanenitrile, the english name: myclobutanil is a systemic triazole fungicide with protection and treatment activity, and the structural formula of the systemic triazole fungicide is shown in figure 1A. The action mechanism is to inhibit the biosynthesis of the ergosterol of phytopathogen. The bactericide has the characteristics of strong systemic property, long lasting period, high pesticide effect, safety to crops, certain stimulation to the growth of the crops and the like. The myclobutanil has good control effect on wheat loose smut, Tilletia reticulata, powdery mildew, rust, cladosporium cucumerinum, gray spot and brown spot.
Fludioxonil [ chinese name: fludioxonil, xerox, 4- (2, 2-difluoro-1, 3-benzodioxo-4-yl) pyrrole-3-carbonitrile; english name: the fluotioxonil is a non-systemic broad-spectrum pyrrole bactericide, and the structural formula of the fluotioxonil is shown in a figure 1B. The bactericide has unique action mechanism, mainly inhibits the transfer related to the phosphorylation of glucose, and inhibits the growth of fungal mycelia, and finally causes the death of pathogenic bacteria. Has no cross resistance with the existing bactericide. Fludioxonil can be used for treating stinking smut, snow rot, snow mold, banded sclerotial blight, root rot, take-all, glume blight and stem smut of wheat; barley stripe disease, net blotch, head smut; maize bacterial wilt, stem rot, damping off; cotton rhizoctonia rot, red rot, anthracnose, black root, seed rot; root rot caused by soybean peanut damping off and fusarium; rice bakanae disease, leaf spot of flax, early leaf blast and damping off; black spot and black shank of rape; potato damping off, scab; the vegetable blight, anthracnose, brown spot, gummy stem blight and the like have good control effects.
Whether myclobutanil, fludioxonil or other high-efficiency bactericides are used independently for a long time, pathogenic bacteria are easy to generate drug resistance, the problems of reduced prevention effect, shortened service life of the bactericides, increased dosage and the like are caused, and the long-term sustainable control of diseases is not facilitated. The method is a common method for relieving the drug resistance of plant pathogenic bacteria by scientifically compounding different single bactericides; by compounding the bactericides with different action mechanisms and target objects, the control effect can be improved, the dosage of the pesticide can be greatly reduced, the bactericidal spectrum can be expanded, the bactericidal efficiency can be improved, the generation of the drug resistance of field pathogenic bacteria can be delayed, and the occurrence of phytotoxicity can be reduced.
Disclosure of Invention
The invention aims to provide a bactericidal composition containing myclobutanil and fludioxonil and application thereof in preventing and controlling the corn macule, wherein a mixed preparation suitable for preventing and controlling the corn macule is obtained through scientific proportioning, and the obtained preparation can obviously inhibit the growth of the mycelium of the corn macule, thereby providing a new technical support for preventing and controlling the occurrence of harm and propagation and spread of diseases of the corn macule.
In order to achieve the purpose, the invention adopts the following technical scheme:
a bactericidal composition containing myclobutanil and fludioxonil, which is prepared from myclobutanil [ the name of chinese: 1- (4-chlorophenyl) -2- (1H-1, 2, 4-triazole-1-methyl) hexanenitrile or 2- (4-chlorophenyl) -2- (1H, 1, 2, 4-triazole-1-methyl) hexanenitrile, the english name: myclobutanil ] and fludioxonil [ chinese name: fludioxonil, xerox, 4- (2, 2-difluoro-1, 3-benzodioxo-4-yl) pyrrole-3-carbonitrile; english name: fludioxonil ] is an active ingredient, wherein the mass ratio of myclobutanil to fludioxonil is 1-9: 9-1, and preferably the mass ratio of myclobutanil to fludioxonil is 9: 1.
The mixed medicament containing the myclobutanil and the fludioxonil can be used for preventing and treating corn microsporum caused by corn microsporum (Bipolarismaydis).
The invention further obtains the mixing proportion with the addition and synergy by compounding the myclobutanil and the fludioxonil. Through a series of indoor toxicity tests, the obtained mixed medicament integrates the advantages of two medicaments with different action mechanisms, thereby effectively controlling the occurrence of the corn microsporum and preventing the occurrence of the drug resistance of pathogenic bacteria.
The bactericidal composition containing the myclobutanil and the fludioxonil has excellent control effect on the corn maculopathy, the pesticide effect of the compound medicament is long in lasting period, and the cost is lower than that of the single use of the myclobutanil or the fludioxonil.
The invention is beneficial to delaying the generation of drug resistance of pathogenic bacteria, can improve the control effect, reduce the dosage and the use cost, solves the problem of drug use in the actual production of corn, has potential social, economic and ecological benefits and has wide application prospect in the field of agricultural bactericides.
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FIG. 1 is the structural formula of myclobutanil and fludioxonil as fungicides; wherein, (A) is myclobutanil, and (B) is fludioxonil.
FIG. 2 shows the toxicity test results of the mixed drug containing myclobutanil and fludioxonil (myclobutanil: fludioxonil is 9: 1) of the invention to the strain NJ1704 of Microsporum zeae, wherein CK is a control group without drug, and the drug concentrations of the other 4 plates are 0.05, 0.25, 1.25 and 6.25 mug/m L respectively.
FIG. 3 shows the control effect of the mixed preparation containing myclobutanil and fludioxonil (myclobutanil: fludioxonil 9: 1) on isolated leaves of corn septoria maculosa, wherein CK is a clear water control group, and the other 3 treatments are myclobutanil and fludioxonil (9: 1), myclobutanil single agent and fludioxonil single agent respectively, and the treatment concentrations are 10 μ g/m L.
Detailed Description
A bactericidal composition containing myclobutanil and fludioxonil, which is prepared from myclobutanil [ the name of chinese: 1- (4-chlorophenyl) -2- (1H-1, 2, 4-triazole-1-methyl) hexanenitrile or 2- (4-chlorophenyl) -2- (1H, 1, 2, 4-triazole-1-methyl) hexanenitrile, the english name: myclobutanil ] and fludioxonil [ chinese name: fludioxonil, xerox, 4- (2, 2-difluoro-1, 3-benzodioxo-4-yl) pyrrole-3-carbonitrile; english name: fludioxonil ] is an active ingredient, wherein the mass ratio of myclobutanil to fludioxonil is 1-9: 9-1, and comprises 1: 9, 2: 8, 3: 7, 4: 6, 5: 5, 6: 4, 7: 3, 8: 2 or 9: 1; preferably, the mass ratio of myclobutanil to fludioxonil is 9: 1.
The compound medicament can be used for preventing and treating corn leaf spot caused by corn leaf spot (Bipolaris maydis).
In order to make the present invention more comprehensible, the technical solutions of the present invention are further described below with reference to specific embodiments, but the present invention is not limited thereto.
EXAMPLE one bacteriostatic Effect of a Compound containing myclobutanil and fludioxonil
1. Preparation of pharmaceutical agents
The myclobutanil (95%) used in this example was obtained from Taizhou Dapeng pharmaceuticals, Inc., and the fludioxonil (98%) used was obtained from Zhejiang Hebei science and technology, Inc.
The method comprises the steps of dissolving raw myclobutanil and fludioxonil respectively with dimethyl sulfoxide to prepare mother liquor of 625 mu g/m L, mixing the mother liquor of myclobutanil and the mother liquor of myclobutanil according to 9 different volume ratios of 1: 9, 2: 8, 3: 7, 4: 6, 5: 5, 6: 4, 7: 3, 8: 2 and 9: 1 to prepare mixed mother liquor, diluting the mixed mother liquor into mixed preparations with serial concentrations by using 0.1% of Tween 80, and setting the concentrations of the mixed preparations to be 0, 0.01, 0.05, 0.25, 1.25 and 6.25 mu g/m L on the basis of a pre-experiment.
2. Bacteriostatic effect of the medicament
The genetic diversity and pathogenicity [ J ] of the above medicament on corn microsporum strain NJ1704 (Pirani, Ganlin, Rana hongkongensis, etc.. propiconazole of Fujian province with different sensitivities are tested in the example, the plant pathology report, 2019, 49 (1): 64-74) has the bacteriostasis effect.
(1) Indoor toxicity determination of single agent and mixed agent on corn small spot pathogen
A. The growth rate method is adopted to respectively determine the bacteriostatic activity of the mixed medicament of the myclobutanil, the fludioxonil and 9 myclobutanil and fludioxonil with different proportions on the corn leaf spot pathogen NJ 1704. Namely, the growth inhibition rate is calculated according to the following formula:
then converting the growth inhibition rate into a several-degree value (Y), converting the medicament concentration (mu g/m L) into a logarithmic value (X), and solving a virulence regression equation (Y ═ a + bX) and a correlation coefficient (r) by using a regression line so as to calculate the effective intermediate concentration EC of the medicament on the bacterial strain NJ1704 of the corn small leaf blight strain50。
B. And (3) measuring the combined toxicity of the agents, calculating the synergistic coefficient (SR) of the mixed agents by using a Wadly method, and evaluating the combined action type of the mixed agents.
SR-mixed chemical theory EC50Actual measurement of EC in mixed medicament50。
When SR is less than 0.5, the mixed medicament has antagonism; when SR is not less than 0.5 and not more than 1.5, the mixed medicament has additive effect, and when SR is more than 1.5, the mixed medicament has synergistic effect.
Indoor toxicity determination of single agents and mixed agents of myclobutanil and fludioxonil on strain NJ1704 of Microsporum maydis is shown in Table 1, wherein toxicity determination of mixed agents of myclobutanil and fludioxonil (9: 1) on strain NJ1704 of Microsporum maydis is shown in FIG. 1.
TABLE 1 indoor toxicity test (growth rate method) for Myosporium zeae by myclobutanil and fludioxonil single agent and mixed agent
As can be known from the results in Table 1 and FIG. 2, 9 mixed agents of myclobutanil and fludioxonil in different proportions have good virulence effects on corn microsporum, wherein the mass ratio of myclobutanil to fludioxonil is 9: 1, and the mixed agents have obvious synergism.
EXAMPLE two in vitro leaf control test of mixed drug containing myclobutanil and fludioxonil against corn septoria
The test is carried out in a key laboratory for monitoring and treating the pests of crops in Fujian province, and the corn variety is the top quality (Fujian province, agricultural development Co., Ltd.).
Application of the medicament: 95% of myclobutanil, Taizhou Dapeng pharmaceutical Co., Ltd; fludioxonil original drug with the concentration of 98 percent, Zhejiang Secale science and technology GmbH; the mixed medicament contains myclobutanil and fludioxonil, wherein the mass ratio of the myclobutanil to the fludioxonil is 9: 1.
The experimental design comprises (1) diluting a single-dose 95% myclobutanil original drug to 200, 100, 50 and 10 mu g/m L respectively, diluting a single-dose 98% fludioxonil original drug to 200, 100, 50 and 10 mu g/m L respectively, (2) sequentially diluting a mixed drug containing myclobutanil and fludioxonil to 200, 100, 50 and 10 mu g/m L, wherein the mass ratio of myclobutanil to fludioxonil is 9: 1, and (3) taking sterile water with the same amount as the application amount as a blank control.
The experimental method comprises the steps of planting tested corn seedlings in a greenhouse cement pond (150cm × 130cm), planting 40 corns in each pond, taking the third leaf of each corn (from top to bottom) when the corn grows to the 6-7 leaf stage, cutting the third leaf into 60mm × 80mm small pieces by using sterile scissors after removing veins, taking 200m L of the agents with different concentrations in a 500m L beaker, immersing the cut corn leaves in liquid medicine for 30s, naturally drying the corn leaves on sterile gauze in an aseptic operating platform for 10min, paving sterile filter paper with the diameter of 90mm in a 90mm culture dish, adding 3m L sterile water for wetting, paving the corn leaves soaked by the agents with different concentrations in the center of the culture dish, slightly puncturing 3 holes in the center of each leaf by using a sterile inoculating needle, punching 3 dark holes in the edge of a small leaf spot of corn with the diameter of 90mm by using a sterile puncher (the diameter of 8mm), punching a bacteria dish on the edge 1704 NJ of the activated corn small leaf with the strains with the different concentrations, inoculating the leaves with the agents, inoculating a single bacterial strain, and repeatedly testing the bacterial colony length of the bacteria on the sterile filter paper, repeating the culture dish, and repeating the test procedure, wherein the bacterial colony length of the bacterial colony of the test paper is 3 mm, and the test paper is calculated for 3 times, and the test effect is calculated for 3 times.
TABLE 2 prevention and control of Ex vivo leaf of corn leaf blotch with different agents
As can be seen from the test results in Table 2 and FIG. 3, the mixed pesticide prepared from 10-200 μ g/m L of myclobutanil and fludioxonil according to the mass ratio of 9: 1 has very excellent control effect on the corn septoria, and the control effect is as high as more than 81%, which is remarkably higher than that of a single pesticide with the same concentration.
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
Claims (5)
1. A germicidal composition, characterized by: the method comprises the following steps of taking myclobutanil and fludioxonil as active ingredients, wherein the mass ratio of the myclobutanil to the fludioxonil is 1-9: 9-1.
2. The germicidal composition of claim 1, wherein: the mass ratio of the myclobutanil to the fludioxonil is 9: 1.
3. The germicidal composition of claim 1, wherein: the mass ratio of the myclobutanil to the fludioxonil is 1: 9.
4. The use of the fungicidal composition according to claim 1 for the control of northern leaf blight, characterized in that: is used for preventing and treating the corn northern leaf blight.
5. The use of the fungicidal composition of claim 4 for controlling northern leaf blight, wherein: the northern leaf blight is caused by southern leaf blight of maize (Bipolaris maydis).
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| CN202010079417.7A CN111449074A (en) | 2020-02-04 | 2020-02-04 | Bactericidal composition containing myclobutanil and fludioxonil and application of bactericidal composition to prevention and treatment of corn macule disease |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005051081A1 (en) * | 2003-11-26 | 2005-06-09 | Syngenta Participations Ag | Method for the protection of materials |
| CN104094937A (en) * | 2014-06-25 | 2014-10-15 | 广东中迅农科股份有限公司 | Sterilization composition containing triflumizole and fludioxonil |
| CN107094769A (en) * | 2017-06-19 | 2017-08-29 | 福建省农业科学院植物保护研究所 | Microbicide compositions and its application containing fludioxonil and RH-7592 |
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- 2020-02-04 CN CN202010079417.7A patent/CN111449074A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005051081A1 (en) * | 2003-11-26 | 2005-06-09 | Syngenta Participations Ag | Method for the protection of materials |
| CN104094937A (en) * | 2014-06-25 | 2014-10-15 | 广东中迅农科股份有限公司 | Sterilization composition containing triflumizole and fludioxonil |
| CN107094769A (en) * | 2017-06-19 | 2017-08-29 | 福建省农业科学院植物保护研究所 | Microbicide compositions and its application containing fludioxonil and RH-7592 |
Non-Patent Citations (3)
| Title |
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| 广东省农业科学院作物研究所、广东省科学技术厅农村科技处编: "《广东甜糯玉米新品种新技术》", 31 August 2011, 广东省出版集团，广东人民出版社 * |
| 张舒等: "《新编农药施用手册》", 30 September 2010, 武汉理工大学出版社 * |
| 郭宁等: "不同种衣剂对玉米茎腐病的防治效果", 《河北农业科学》 * |
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Application publication date: 20200728 |