CN111436607A - 一种抑制人结直肠癌细胞sw480增殖的玫瑰花提取物及其应用 - Google Patents
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Abstract
本发明涉及一种抑制人结直肠癌细胞SW480增殖的玫瑰花提取物及其应用，属于食品加工技术领域。该玫瑰花提取物经如下步骤得到：新鲜食用玫瑰花在40‑80℃下烘干，将其超微粉碎，按粉碎后的玫瑰花:沸水=1g：1‑200mL的比例浸提30分钟，过滤得到滤液，重复上述浸提步骤2次，合并滤液，滤液经传统的浓缩、干燥后即得。细胞试验表明，此玫瑰花提取物能显著抑制人结直肠癌细胞SW480的增殖，能作为爱吃红肉人士的健康伴侣，应用于食品和保健品中。
Description
技术领域
本发明属于食品加工技术领域，具体的说，涉及一种抑制人结直肠癌细胞SW480增殖的玫瑰花提取物及其应用。
背景技术
结直肠癌是世界常见的恶性肿瘤之一，据国际癌症研究理事会最新统计，仅2013年就新增结直肠癌患者102480例，因结直肠癌而死亡的人数达50 830例。30年来，我国结直肠癌发病率年均上升3％～4％。我国结直肠癌发病率和死亡例数分别占全世界发病和死亡总例数的18.6％和20.1％，均居第1位。尽管当前结肠癌治疗方面涌出一些新的方法，如西妥昔单抗、贝伐单抗等分子靶向药物，但总体上来看结肠癌治疗效果仍然较差，尚无满意的非侵入性治疗方案。
现代研究证明，玫瑰花能够抗氧化、抗衰老及降血糖，能够平衡及强化胃肠道功能，调节消化液的分泌，促进消化和吸收；对消化不良及胃酸分泌过多等慢性疾患有一定的调节和改善作用；对情绪紧张、压抑等引起的胃痛有明显的改善功能。本发明以玫瑰花为原料，制备一种具有抑制人结直肠癌细胞SW480增殖的玫瑰花提取物。经文献检索，未见与本发明相同的公开报道。
发明内容
本发明是目的在于提供一种抑制人结直肠癌细胞SW480增殖的玫瑰花提取物及其应用，
为实现上述目的，本发明是通过如下技术方案实现的：
所述的玫瑰花提取物，采用水浸提法制备而得，具体包括以下步骤：新鲜食用玫瑰花在40-80℃下烘干，将其超微粉碎，按粉碎后的玫瑰花:沸水＝1g：1-200mL的比例浸提1-60分钟，过滤得到滤液，重复上述浸提步骤2次，合并滤液，滤液经浓缩、干燥后，即得玫瑰花提取物。
进一步优选，所述干燥采用真空冷冻干燥。
进一步优选，所述干燥采用40-80℃条件下烘干处理。
玫瑰花提取物在抑制人结直肠癌细胞SW480增殖产品中的应用，所述玫瑰花提取物为冻干粉或烘干粉。
玫瑰花提取物在抑制人结直肠癌细胞SW480增殖产品中的应用，所述玫瑰花提取物是利用上述制备方法获得。
玫瑰花提取物在食品和保健品中的应用，所述玫瑰花提取物为冻干粉或烘干粉。
玫瑰花提取物在食品和保健品中的应用，所述玫瑰花提取物是利用上述制备方法获得。
本发明的有益效果：该玫瑰花提取物富含多酚类、黄酮类、多糖，能抑制人结直肠癌细胞SW480的增殖，可应用于食品和保健品中。
具体实施方式
为了使本发明的目的、技术方案和有益效果更加清楚，下面将对本发明的优选实施例进行详细的说明，以方便技术人员理解。
【实施例1】
玫瑰花提取物，采用水浸提法制备而得，具体步骤如下：新鲜食用玫瑰花在65℃下烘干后将其超微粉碎，按粉碎后的玫瑰花:沸水＝1g：10mL的比例浸提30分钟，过滤得到滤液，重复上述浸提步骤2次，合并滤液，滤液减压浓缩后，经真空冷冻干燥，得到玫瑰花提取物(冻干)。
【实施例2】
玫瑰花提取物，采用水浸提法制备而得，具体步骤如下：新鲜食用玫瑰花在65℃下烘干后将其超微粉碎，按粉碎后的玫瑰花:沸水＝1g：10mL的比例浸提30分钟，过滤得到滤液，重复上述浸提步骤2次，合并滤液，滤液减压浓缩后，浓缩液在65℃下烘干得到玫瑰花提取物(烘干)。
实施分析
将实施例1、2得到的玫瑰花提取物进行细胞试验，用MTT法检测玫瑰花提取物对人结直肠癌细胞SW480增殖的影响，其结果如下：采用不同浓度的玫瑰花提取物(冻干)培养SW480细胞，随着培养时间的延长，细胞相对存活率降低；培养时间达72h，玫瑰花提取物(冻干)浓度≥400mg/l时，SW480细胞存活率显著降低，表明其能显著抑制人结直肠癌细胞SW480增殖。采用不同浓度的玫瑰花提取物(烘干)培养SW480细胞，培养时间仅为24h时，细胞存活率即显著降低。培养时间为24h和48h时，不同浓度玫瑰花提取物(烘干)对SW480细胞的存活率无显著性差异；培养时间为72h时，玫瑰花提取物(烘干)浓度越高，则细胞存活率越低，显示出较好的剂量效应关系。表1表明，玫瑰花提取物(烘干)对人结直肠癌细胞SW480增殖的抑制优于玫瑰花提取物(冻干)。
表1玫瑰花提取物对人结直肠癌细胞SW480相对存活率的影响
注：同列间大写字母肩标相同者，差异不显著(P>0.05)，同列间大写字母肩标不同者，差异显著(P<0.05)；同行间小写字母肩标相同者，差异不显著(P>0.05)，同行间小写字母肩标不同者，差异显著(P<0.05)。
研究结果表明：玫瑰花提取物能抑制人结直肠癌细胞SW480的增殖，玫瑰花提取物(烘干)对人结直肠癌细胞SW480增殖的抑制优于玫瑰花提取物(冻干)。玫瑰花提取物能直接加工成粉剂、胶囊、含片及饮料等，使用方便，便于携带；也能够作为添加剂用于食品中。
最后说明的是，以上优选实施例仅用于说明本发明的技术方案而非限制，尽管通过上述优选实施例已经对本发明进行了详细的描述，但本领域技术人员应当理解，可以在形式上和细节上对其作出各种各样的改变，而不偏离本发明权利要求书所限定的范围。
Claims (7)
1.一种玫瑰花提取物，其特征在于：其制备方法包括以下步骤：新鲜食用玫瑰花在40-80℃下烘干，将其超微粉碎，按粉碎后的玫瑰花:沸水=1g：1-200mL的比例浸提1-60分钟，过滤得到滤液，重复上述浸提步骤2次，合并滤液，滤液经浓缩、干燥后，即得玫瑰花提取物。
2.根据权利要求1所述的一种玫瑰花提取物，其特征在于：所述干燥采用真空冷冻干燥。
3.根据权利要求1所述的一种玫瑰花提取物，其特征在于：所述干燥采用40-80℃条件下烘干处理。
4.玫瑰花提取物在抑制人结直肠癌细胞SW480增殖产品中的应用，其特征在于：所述玫瑰花提取物为冻干粉或烘干粉。
5.玫瑰花提取物在抑制人结直肠癌细胞SW480增殖产品中的应用，其特征在于：所述玫瑰花提取物是利用权利要求1-3任一项所述的制备方法获得。
6.玫瑰花提取物在食品和保健品中的应用，其特征在于：所述玫瑰花提取物为冻干粉或烘干粉。
7.玫瑰花提取物在食品和保健品中的应用，其特征在于：所述玫瑰花提取物是利用权利要求1-3任一项所述的制备方法获得。
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| TW200825177A (en) * | 2006-12-04 | 2008-06-16 | Chuan Bio Tech Corp I | Co-fermentation method of phellinus linteus and Chinese herbal medicine |
| CN106070992A (zh) * | 2016-06-27 | 2016-11-09 | 安徽省怀宁县顶雪食品有限公司 | 一种适合女性食用的酥糖及其制备方法 |
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Application publication date: 20200724 |