CN111406744B - General sex pheromone composition for ectropis obliqua and tea caterpillar, sex attractant, and preparation method and application thereof - Google Patents
General sex pheromone composition for ectropis obliqua and tea caterpillar, sex attractant, and preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/20—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom three- or four-membered rings
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Abstract
The invention provides a general sex pheromone composition for tea geometrid and tea caterpillar, a sex attractant and a preparation method and application thereof, and belongs to the technical field of tea tree pest control, wherein the general sex pheromone composition comprises cis 3, cis 6, cis 9-octadecatriene, cis 3, cis 9-6, 7-epoxyoctadecadiene, cis 3, cis 6-9, 10-epoxyoctadecadiene, cis 6, cis 9-3, 4-epoxyoctadecadiene, 10, 14-dimethyl pentadecanol isobutyrate and 14-methyl pentadecanol isobutyrate in a mass ratio of (20-70) - (30-80) - (25-75) - (60-100) - (5-30). The general attractant has strong attraction to both the ectropis obliqua and the tea caterpillars, and can efficiently carry out population monitoring, trapping, killing and preventing on the ectropis obliqua and the tea caterpillars.
Description
Technical Field
The invention belongs to the technical field of tea plant pest control, and particularly relates to a general sex pheromone composition for ectropis grisescens and tea caterpillars, a sex attractant, and a preparation method and application thereof.
Background
Gray tea geometrid (Ectropis grissescens Warren) and tea caterpillars (Euproctis pseudoconspersa Strand) are two of the most serious tea plant leaf-eating pests. The looper obliqua is distributed in tea areas in southern areas such as Hunan, Hubei, Jiangxi, Zhejiang, Guangdong and Yunnan, particularly, a part in Hunan is seriously harmful, a large number of larvae chew on leaves, and the tea tufts are taken to eat until baldness occurs during outbreak. The tea caterpillars, besides tea trees, also harm various crops such as tea-oil trees, oranges, camellia, Chinese tallow trees, corns and the like, and are mainly distributed in Shaanxi, Jiangsu, Anhui, Zhejiang, Fujian, Taiwan, Guangdong, Guangxi, Jiangxi, Hubei, Hunan, Sichuan, Guizhou and other places. The method mainly occurs in mountain tea gardens, and gradually has a tendency of spreading to mountain tea areas outside mountains and even bursting into disasters in recent years. When the larvae bite the leaves, the larvae can eat the leaves and the tea yield and tree vigor are affected; toxic hair on the larva body touches the skin of the human body, so that the larva body is red, swollen, painful and itchy, and the management of a tea garden is seriously influenced.
The sex pheromone of the insect is a trace chemical substance which is secreted in vitro by a special secretion organ of a sex individual of the same insect, can be accepted by a receptor of an opposite individual of the same species, and causes the opposite individual to generate certain behavioral response or physiological effect (such as mating, directional mating, mating and the like). As an insect coupling communication signal substance, sex pheromone has the important characteristic of specificity, which indicates that the technology is safe to beneficial organisms such as natural enemies, but also notes that the technology has narrow insecticidal spectrum.
The sex pheromone of ectropis grisescens and tea caterpillars has the problem in the application process. The generation areas of the ectropis obliqua and the tea caterpillars are highly overlapped, and two pests in a plurality of tea gardens occur simultaneously. In chemical control, the confusion effect of two inchworms is not large, the control effect is basically similar, but problems occur when sex pheromones with strong specificity are used. When the sex attractant for the ectropis obliqua is used independently, the sex attractant has a good control effect on the ectropis obliqua, but the tea caterpillar can not be effectively controlled, and other measures still need to be taken; when the tea caterpillar sex attractant is used alone, the tea caterpillar sex attractant has a good control effect on tea caterpillar, but ash tea geometrid can not be effectively controlled, and other measures also need to be taken. Therefore, tea growers need an attractant which can simultaneously have effects on the looper and the tea caterpillar in the actual production.
Disclosure of Invention
In view of the above, the invention aims to provide a general sex pheromone composition for ectropis grisescens and tea caterpillars, a sex attractant, a preparation method and an application thereof.
In order to achieve the above purpose, the invention provides the following technical scheme:
a sex pheromone composition universal for gray tea geometrid and tea caterpillar comprises cis-3, cis-6, cis-9-octadecatriene, cis-3, cis-9-6, 7-epoxy octadecadienol, cis-3, cis-6-9, 10-epoxy octadecadienol, cis-6, cis-9-4-epoxy octadecadienol, 10, 14-dimethyl pentadecanol isobutyrate and 14-methyl pentadecanol isobutyrate in a mass ratio of (20-70): (30-80): (25-75): (25-75): 60-100): 5-30.
Preferably, the mass ratio of the cis 3, cis 6, cis 9-octadecatriene, the cis 3, cis 9-6, 7-epoxy octadecadiene, the cis 3, cis 6-9, 10-epoxy octadecadiene, the cis 6, cis 9-3, 4-epoxy octadecadiene, the 10, 14-dimethyl pentadecanol isobutyrate and the 14-methyl pentadecanol isobutyrate is 50:50:50:50:90: 10.
The invention provides a general sex attractant for ectropis obliqua and tea caterpillars, which comprises a general sex pheromone composition and a carrier; the universal sex pheromone composition is placed in a carrier.
Preferably, the general-purpose sex pheromone composition is used in the form of a sex attractant solution, and the solvent of the sex attractant solution is n-hexane.
Preferably, the use concentration of the sex attractant solution is 8-12 mug/muL.
Preferably, the sex attractant solution is used at a concentration of 10. mu.g/. mu.L.
Preferably, the carrier is a bell-shaped rubber plug.
The invention provides a preparation method of the universal sex attractant, which comprises the following steps:
1) cis-3, cis-6, cis-9-octadecatriene, cis-3, cis-9-6, 7-epoxy octadecadienol, cis-3, cis-6-9, 10-epoxy octadecadienol, cis-6, cis-9-3, 4-epoxy octadecadienol, 10, 14-dimethyl pentadecyl isobutyrate and 14-methyl pentadecyl isobutyrate are mixed to prepare a sex attractant solution;
2) soaking the carrier in absolute ethyl alcohol, and drying to obtain a pretreated carrier;
3) placing the sex attractant solution in a pretreatment carrier, and standing for 2-4 h to obtain a universal sex attractant;
there is no chronological definition between steps 1) and 2).
Preferably, the addition amount of each pretreatment carrier neutral attractant solution in the step 3) is 300-340 mu L.
The invention provides the universal sex pheromone composition and the application of the universal sex attractant in the control of ectropis grisescens and tea caterpillars
The invention has the beneficial effects that: the invention provides a general sex pheromone composition for gray tea geometrid and tea caterpillar, which comprises cis 3, cis 6, cis 9-octadecatriene, cis 3, cis 9-6, 7-epoxy octadecadienol, cis 3, cis 6-9, 10-epoxy octadecadienol, cis 6, cis 9-3, 4-epoxy octadecadienol, 10, 14-dimethyl pentadecyl isobutyrate and 14-methyl pentadecyl isobutyrate; the effect of simultaneously attracting two pests, namely the ectropis obliqua and the tea caterpillar, by the lure core can be realized; has stronger trapping capacity on both the ectropis obliqua and the tea caterpillar males, and the trapping effect is equivalent to that of commercial sex attractant.
Detailed Description
The invention provides a general sex pheromone composition for gray tea geometrid and tea caterpillar insects, which comprises cis-3, cis-6, cis-9-octadecatriene, cis-3, cis-9-6, 7-epoxy octadecadiene, cis-3, cis-6-9, 10-epoxy octadecadiene, cis-6, cis-9-4-epoxy octadecadiene, 10, 14-dimethyl pentadecyl isobutyrate and 14-methyl pentadecyl isobutyrate in a mass ratio of (20-70) - (30-80) - (25-75) - (60-100) - (5-30). In the present invention, the universal sex pheromone composition is capable of attracting both asheer and tea caterpillar males.
In the present invention, the mass ratio of cis 3, cis 6, cis 9-octadecatriene, cis 3, cis 9-6, 7-epoxyoctadecadiene, cis 3, cis 6-9, 10-epoxyoctadecadiene, cis 6, cis 9-3, 4-epoxyoctadecadiene, 10, 14-dimethylpentadecanol isobutyrate to 14-methylpentadecanol isobutyrate is preferably 50:50:50:50:90: 10.
The sources of cis-3, cis-6, cis-9-octadecatriene, cis-3, cis-9-6, 7-epoxyoctadecadien, cis-3, cis-6-9, 10-epoxyoctadecadien, cis-6, cis-9-3, 4-epoxyoctadecadien, 10, 14-dimethyl pentadecanol isobutyrate and 14-methyl pentadecanol isobutyrate are not particularly limited, and commercially available products can be adopted.
The invention also provides a general sex attractant for ectropis obliqua and tea caterpillars, which comprises the general sex pheromone composition and a carrier; the universal sex pheromone composition is placed in a carrier.
In the present invention, the general-purpose sex pheromone composition is preferably used in the form of a sex attractant solution, the solvent of which is preferably n-hexane; the use concentration of the sex attractant solution is preferably 8-12 mug/muL, and more preferably 10 mug/muL.
In the present invention, the carrier is preferably a bell-shaped rubber stopper; in the invention, the height of the carrier is preferably 13.5-14.5 mm, more preferably 14mm, and the outer diameter of the sex attractant solution adding port of the carrier is preferably 9.8-10.2 mm, more preferably 10 mm; the inner diameter of the sex attractant solution adding port of the carrier is preferably 7.3-7.8 mm, and more preferably 7.5 mm.
The invention provides a preparation method of the universal sex attractant, which comprises the following steps: 1) cis-3, cis-6, cis-9-octadecatriene, cis-3, cis-9-6, 7-epoxy octadecadienol, cis-3, cis-6-9, 10-epoxy octadecadienol, cis-6, cis-9-3, 4-epoxy octadecadienol, 10, 14-dimethyl pentadecyl isobutyrate and 14-methyl pentadecyl isobutyrate are mixed to prepare a sex attractant solution; 2) soaking the carrier in absolute ethyl alcohol, and drying to obtain a pretreated carrier; 3) placing the sex attractant solution in a pretreatment carrier, and standing for 2-4 h to obtain a universal sex attractant; there is no chronological definition between steps 1) and 2).
In the present invention, cis 3, cis 6, cis 9-octadecatriene, cis 3, cis 9-6, 7-epoxyoctadecadiene, cis 3, cis 6-9, 10-epoxyoctadecadiene, cis 6, cis 9-3, 4-epoxyoctadecadiene, 10, 14-dimethylpentadecanol isobutyrate and 14-methylpentadecanol isobutyrate were mixed to prepare a sex attractant solution. In the present invention, it is preferable that the sex attractant solution is obtained by mixing cis 3, cis 6, cis 9-octadecatriene, cis 3, cis 9-6, 7-epoxyoctadecadiene, cis 3, cis 6-9, 10-epoxyoctadecadiene, cis 6, cis 9-3, 4-epoxyoctadecadiene, 10, 14-dimethyl pentadecanol isobutyrate and 14-methyl pentadecanol isobutyrate, and dissolving them in n-hexane. In the present invention, the n-hexane is preferably a commercially available chromatographically pure hexane.
In the invention, the carrier is soaked in absolute ethyl alcohol and dried to obtain the pretreated carrier. In the invention, the soaking time is preferably 20-28 h, and more preferably 24 h; the role of the soaking is to desulfurize. In the invention, the drying temperature is preferably 38-42 ℃, more preferably 40 ℃, and the drying time is preferably 20-28 h, more preferably 24 h. In the present invention, the drying is preferably performed in an oven.
In the invention, the sex attractant solution is placed in a pretreatment carrier and is kept stand for 2-4 h to obtain the universal sex attractant. In the invention, the addition amount of the neutral attractant solution in each pretreatment carrier is preferably 280-340 mu L, and more preferably 300 mu L. In the present invention, the time for the standing is preferably 3 hours, the standing is preferably performed in a fume hood, and the temperature for the standing is not particularly limited, and the standing may be performed at room temperature (20 to 30 ℃).
The invention also provides the universal sex pheromone composition and application of the universal sex pheromone composition in the control of the griseofulvin and the tea caterpillars. In the invention, the universal sex pheromone composition and the universal sex attractant can simultaneously trap the male worms of the ectropis grisescens and the tea caterpillars, and realize the trapping, killing and control of the ectropis grisescens and the tea caterpillars.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
1) The components are as follows: cis 3, cis 6, cis 9-octadecatriene, cis 3, cis 9-6, 7-epoxyoctadecadiene, cis 3, cis 6-9, 10-epoxyoctadecadiene, cis 6, cis 9-3, 4-epoxyoctadecadiene, 10, 14-dimethyl pentadecanol isobutyrate and 14-methyl pentadecanol isobutyrate, wherein the content of each lure active component is 3400 mu g.
2) The carrier raw material requirements are as follows: the slow release carrier is a rubber plug; the height of the carrier is 14.0 plus or minus 0.5mm, and the outer diameter of the sex pheromone adding port is 10.0 plus or minus 0.2 mm; the inner diameter is 7.5mm plus or minus 0.2 mm; the rubber stopper is a commercially available polyisoprene bell-shaped rubber stopper.
The preparation method comprises the following steps:
1) preparation of the sustained-release carrier: soaking the rubber plug in absolute ethyl alcohol for 24 hours for desulfurization, and putting the taken rubber plug into a drying oven at 40 ℃ for 24 hours;
2) preparing a solution of the sex attractant: respectively adding chromatographically pure hexane into cis 3, cis 6, cis 9-octadecatriene, cis 3, cis 9-6, 7-epoxyoctadecadiene, cis 3, cis 6-9, 10-epoxyoctadecadiene, cis 6, cis 9-3, 4-epoxyoctadecadiene, 10, 14-dimethyl pentadecanol isobutyrate and 14-methyl pentadecanol isobutyrate to be uniformly dissolved to obtain 10 mu g/mu L solution, and mixing according to the volume ratio of 30:70:70:70:70:30 for later use;
3) making of lure
Dripping 340 mu L of the sex attractant solution prepared in the step 2) into the slow-release carrier prepared in the step 1), and then placing the mixture in a fume hood for standing for 3 hours at room temperature to prepare the general sex attractant for the ectropis obliqua and the tea caterpillars.
Example 2
1) The components are as follows: cis 3, cis 6, cis 9-octadecatriene, cis 3, cis 9-6, 7-epoxy octadecadiene, cis 3, cis 6-9, 10-epoxy octadecadiene, cis 6, cis 9-3, 4-epoxy octadecadiene, 10, 14-dimethyl pentadecanol isobutyrate and 14-methyl pentadecanol isobutyrate, wherein the content of each lure active component is 3200 mug.
2) The carrier raw material requirements are as follows: the slow release carrier is a rubber plug; the height of the carrier is 14.0 plus or minus 0.5mm, and the outer diameter of the sex pheromone adding port is 10.0 plus or minus 0.2 mm; the inner diameter is 7.5mm plus or minus 0.2 mm; the rubber stopper is a commercially available polyisoprene bell-shaped rubber stopper.
The preparation method comprises the following steps:
1) preparation of the sustained-release carrier: soaking the rubber plug in absolute ethyl alcohol for 24 hours for desulfurization, and putting the taken rubber plug into a drying oven at 40 ℃ for 24 hours;
2) preparing a solution of the sex attractant: respectively adding chromatographically pure hexane into cis 3, cis 6, cis 9-octadecatriene, cis 3, cis 9-6, 7-epoxyoctadecadiene, cis 3, cis 6-9, 10-epoxyoctadecadiene, cis 6, cis 9-3, 4-epoxyoctadecadiene, 10, 14-dimethyl pentadecanol isobutyrate and 14-methyl pentadecanol isobutyrate to be uniformly dissolved to obtain 10 mu g/mu L solution, and mixing according to the volume ratio of 40:60:60:60:80:20 for later use;
3) making of lure
Dripping 320 mu L of the sex attractant solution prepared in the step 2) into the slow-release carrier prepared in the step 1), and then placing the mixture in a fume hood at room temperature for standing for 3h to prepare the general sex attractant for the ectropis obliqua and the tea caterpillars.
Example 3
1) The components are as follows: cis 3, cis 6, cis 9-octadecatriene, cis 3, cis 9-6, 7-epoxy octadecadiene, cis 3, cis 6-9, 10-epoxy octadecadiene, cis 6, cis 9-3, 4-epoxy octadecadiene, 10, 14-dimethyl pentadecanol isobutyrate and 14-methyl pentadecanol isobutyrate, wherein the content of each lure active component is 3000 mu g.
2) The carrier raw material requirements are as follows: the slow release carrier is a rubber plug; the height of the carrier is 14.0 plus or minus 0.5mm, and the outer diameter of the sex pheromone adding port is 10.0 plus or minus 0.2 mm; the inner diameter is 7.5mm plus or minus 0.2 mm; the rubber stopper is a commercially available polyisoprene bell-shaped rubber stopper.
The preparation method comprises the following steps:
1) preparation of the sustained-release carrier: soaking the rubber plug in absolute ethyl alcohol for 24 hours for desulfurization, and putting the taken rubber plug into a drying oven at 40 ℃ for 24 hours;
2) preparing a solution of the sex attractant: respectively adding chromatographically pure hexane into cis 3, cis 6, cis 9-octadecatriene, cis 3, cis 9-6, 7-epoxyoctadecadiene, cis 3, cis 6-9, 10-epoxyoctadecadiene, cis 6, cis 9-3, 4-epoxyoctadecadiene, 10, 14-dimethyl pentadecanol isobutyrate and 14-methyl pentadecanol isobutyrate to be uniformly dissolved to obtain 10 mu g/mu L solution, and mixing according to the volume ratio of 50:50:50:50:90:10 for later use;
3) making of lure
Dripping 300 mu L of the sex attractant solution prepared in the step 2) into the slow-release carrier prepared in the step 1), and then placing the mixture in a fume hood at room temperature for standing for 3h to prepare the general sex attractant for the ectropis obliqua and the tea caterpillars.
Example 4
1) The components are as follows: cis 3, cis 6, cis 9-octadecatriene, cis 3, cis 9-6, 7-epoxy octadecadiene, cis 3, cis 6-9, 10-epoxy octadecadiene, cis 6, cis 9-3, 4-epoxy octadecadiene, 10, 14-dimethyl pentadecanol isobutyrate and 14-methyl pentadecanol isobutyrate, wherein the content of each lure active component is 3000 mu g.
2) The carrier raw material requirements are as follows: the slow release carrier is a rubber plug; the height of the carrier is 14.0 plus or minus 0.5mm, and the outer diameter of the sex pheromone adding port is 10.0 plus or minus 0.2 mm; the inner diameter is 7.5mm plus or minus 0.2 mm; the rubber stopper is a commercially available polyisoprene bell-shaped rubber stopper.
The preparation method comprises the following steps:
1) preparation of the sustained-release carrier: soaking the rubber plug in absolute ethyl alcohol for 24 hours for desulfurization, and putting the taken rubber plug into a drying oven at 40 ℃ for 24 hours;
2) preparing a solution of the sex attractant: respectively adding chromatographically pure hexane into cis 3, cis 6, cis 9-octadecatriene, cis 3, cis 9-6, 7-epoxyoctadecadiene, cis 3, cis 6-9, 10-epoxyoctadecadiene, cis 6, cis 9-3, 4-epoxyoctadecadiene, 10, 14-dimethyl pentadecanol isobutyrate and 14-methyl pentadecanol isobutyrate to be uniformly dissolved to obtain 10 mu g/mu L solution, and mixing according to the volume ratio of 50:50:50:50:80:20 for later use;
3) making of lure
Dripping 300 mu L of the sex attractant solution prepared in the step 2) into the slow-release carrier prepared in the step 1), and then placing the mixture in a fume hood at room temperature for standing for 3h to prepare the general sex attractant for the ectropis obliqua and the tea caterpillars.
Example 5
1) The components are as follows: cis 3, cis 6, cis 9-octadecatriene, cis 3, cis 9-6, 7-epoxy octadecadiene, cis 3, cis 6-9, 10-epoxy octadecadiene, cis 6, cis 9-3, 4-epoxy octadecadiene, 10, 14-dimethyl pentadecanol isobutyrate and 14-methyl pentadecanol isobutyrate, wherein the content of each lure active component is 3000 mu g.
2) The carrier raw material requirements are as follows: the slow release carrier is a rubber plug; the height of the carrier is 14.0 plus or minus 0.5mm, and the outer diameter of the sex pheromone adding port is 10.0 plus or minus 0.2 mm; the inner diameter is 7.5mm plus or minus 0.2 mm; the rubber stopper is a commercially available polyisoprene bell-shaped rubber stopper.
The preparation method comprises the following steps:
1) preparation of the sustained-release carrier: soaking the rubber plug in absolute ethyl alcohol for 24 hours for desulfurization, and putting the taken rubber plug into a drying oven at 40 ℃ for 24 hours;
2) preparing a solution of the sex attractant: respectively adding chromatographically pure hexane into cis 3, cis 6, cis 9-octadecatriene, cis 3, cis 9-6, 7-epoxyoctadecadiene, cis 3, cis 6-9, 10-epoxyoctadecadiene, cis 6, cis 9-3, 4-epoxyoctadecadiene, 10, 14-dimethyl pentadecanol isobutyrate and 14-methyl pentadecanol isobutyrate to be uniformly dissolved to obtain 10 mu g/mu L solution, and mixing according to the volume ratio of 50:50:50:50:70:30 for later use;
3) making of lure
Dripping 300 mu L of the sex attractant solution prepared in the step 2) into the slow-release carrier prepared in the step 1), and then placing the mixture in a fume hood at room temperature for standing for 3h to prepare the general sex attractant for the ectropis obliqua and the tea caterpillars.
Example 6
The general sex attractant trapping test of the griseofulvin and the tea caterpillar is carried out in a tea base of Yucha tea GmbH of Shaoxing city in Zhejiang province. The sex attractant used in the test is the sex attractant in the embodiment 1-5, and the blank control is a rubber plug added with n-hexane. The sex attractant was used in conjunction with boat traps, 3 replicates per treatment. Boat-shaped traps were randomly arranged in a checkerboard fashion in the tea garden, each trap was 25cm above the tea plant, with trap spacing of 15 m. From the survey data in tables 1 and 2, it can be seen that the universal sex attractant combined with the boat type traps have trapping ability for both true gray tea geometrid and tea caterpillar males.
TABLE 1 Laurencia griseola and Thea sinensis general type attractant first field trap test
TABLE 2 Ash tea geometrid and tea caterpillar general type attractant for second field trapping test
The general sex attractant for the griseofulvara and the tea caterpillar is compared with the conventional sex attractant for the griseofulvara and the tea caterpillar and is subjected to trapping tests in a tea base of Yucha tea limited company in Shaoxing city in Zhejiang province. The sex attractant used in the test is prepared by the scheme of the embodiment 3 of the invention, and the contrast lure is a commercial gray tea geometrid sex attractant and a tea caterpillar sex attractant. The sex attractant was used in conjunction with boat traps, 3 replicates per treatment. Boat-shaped traps are randomly arranged in a tea garden in a checkerboard mode, each trap is 25cm higher than a tea tree, the interval between the traps is 15m, and the number of trapped insects is counted for 4 times. As can be seen from the investigation data in Table 3, the commercial looper sex attractant can specifically attract the looper males, the commercial looper sex attractant can specifically attract the tea caterpillars, and the general sex attractant has stronger trapping ability for both loopers and loopers in combination with the boat-type trapper.
TABLE 3 general sex pheromone vs. commercial conventional sex pheromone
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A sex pheromone composition universal for gray tea geometrid and tea caterpillar is characterized by comprising cis-3, cis-6, cis-9-octadecatriene, cis-3, cis-9-6, 7-epoxy octadecadiene, cis-3, cis-6-9, 10-epoxy octadecadiene, cis-6, cis-9, 4-epoxy octadecadiene, 10, 14-dimethyl pentadecanol isobutyrate and 14-methyl pentadecanol isobutyrate in a mass ratio of (20-70) - (30-80) - (25-75) - (60-100) - (5-30).
2. The universal sex pheromone composition according to claim 1, wherein the mass ratio of cis 3, cis 6, cis 9-octadecatriene, cis 3, cis 9-6, 7-epoxyoctadecadien, cis 3, cis 6-9, 10-epoxyoctadecadien, cis 6, cis 9-3, 4-epoxyoctadecadien, 10, 14-dimethylpentadecanol isobutyrate and 14-methylpentadecanol isobutyrate is 50:50:50:50:90: 10.
3. A versatile sex attractant for ectropis grisescens and tea caterpillars comprising the versatile sex pheromone composition of claim 1 or 2 and a carrier; the universal sex pheromone composition is placed in a carrier.
4. The universal sex pheromone composition according to claim 3, wherein the universal sex pheromone composition is used in the form of a sex attractant solution, and the solvent of the sex attractant solution is n-hexane.
5. The universal sex attractant of claim 4, wherein the concentration of the sex attractant solution is 8-12 μ g/μ L.
6. The universal sex attractant of claim 5, wherein the sex attractant solution is used at a concentration of 10 μ g/μ L.
7. The universal sex attractant of claim 3 wherein the carrier is a bell-shaped rubber stopper.
8. The method for preparing the universal sex attractant of any one of claims 3 to 7, comprising the steps of:
1) cis-3, cis-6, cis-9-octadecatriene, cis-3, cis-9-6, 7-epoxy octadecadienol, cis-3, cis-6-9, 10-epoxy octadecadienol, cis-6, cis-9-3, 4-epoxy octadecadienol, 10, 14-dimethyl pentadecyl isobutyrate and 14-methyl pentadecyl isobutyrate are mixed to prepare a sex attractant solution;
2) soaking the carrier in absolute ethyl alcohol, and drying to obtain a pretreated carrier;
3) placing the sex attractant solution in a pretreatment carrier, and standing for 2-4 h to obtain a universal sex attractant;
there is no chronological definition between steps 1) and 2).
9. The preparation method according to claim 8, wherein the addition amount of each pretreatment carrier neutral attractant solution in step 3) is 300 to 340. mu.L.
10. The use of the universal sex pheromone composition as claimed in claim 1 or 2 and the universal sex attractant as claimed in any one of claims 3 to 7 for the control of ectropis grisea and tea caterpillars.
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Application publication date: 20200714 Assignee: HANGZHOU YIHAO AGRICULTURAL TECHNOLOGY CO.,LTD. Assignor: TEA Research Institute CHINESE ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2022330000481 Denomination of invention: A kind of general sex pheromone composition of gray tea inchworm and tea caterpillar, sex attractant and preparation method and application thereof Granted publication date: 20210625 License type: Exclusive License Record date: 20220830 |