CN111388393A

CN111388393A - Bacteriostatic toothpaste containing bamboo shaving triterpenes and preparation method thereof

Info

Publication number: CN111388393A
Application number: CN202010315279.8A
Authority: CN
Inventors: 姜春鹏; 张英; 张明磊
Original assignee: Shanghai Lvrui Biotechnology Co ltd
Current assignee: Shanghai Lvrui Biotechnology Co ltd
Priority date: 2020-04-21
Filing date: 2020-04-21
Publication date: 2020-07-10

Abstract

The invention relates to the technical field of daily chemical products, in particular to bacteriostatic toothpaste containing bamboo shaving triterpenes and a preparation method thereof. The natural healthy, green and safe botanical drug, namely the bamboo shaving triterpenes, is used as a raw material, and the abrasive, the phosphate, the solubilizer and the like in a synergistic system are used for preparing the natural antibacterial toothpaste, so that the prepared antibacterial toothpaste can effectively inhibit the growth of common oral pathogenic bacteria, such as porphyromonas gingivalis, modified streptococcus, candida albicans, helicobacter pylori, staphylococcus aureus, escherichia coli and the like, can remove part of odor-causing bacteria and residual odor-causing substrates in the oral cavity, and can inhibit the growth of pathogenic bacteria, such as cariogenic bacteria, periodontal pathogenic bacteria and oral lichen planus. The bacteriostatic toothpaste containing the triterpene from bamboo shavings has the advantages that the special type of abrasive agent hydrated silica and tetrasodium pyrophosphate are added, and the biological membrane on the surface of the tooth is removed through mechanical friction and desorption. The two have synergistic effect, and the barrier of the prior art is solved.

Description

Bacteriostatic toothpaste containing bamboo shaving triterpenes and preparation method thereof

Technical Field

The invention relates to the technical field of daily chemical products, in particular to bacteriostatic toothpaste containing bamboo shaving triterpenes and a preparation method thereof.

Background

Oral health conditions of people in China are not optimistic, caries rate is increased year by year, and oral problems such as gingival bleeding and periodontitis commonly occur. The oral cavity is one of the main entrances of the human body which is directly contacted with the external environment, the moist heat and the warmth of the internal environment are suitable for the survival and the proliferation of various flora, so that the distribution of various external invasion and the flora in the body is dense, oral diseases such as dental caries, periodontitis, halitosis and the like are mainly caused by pathogenic bacteria, Streptococcus mutans (Streptococcus mutans) and Streptococcus sobrinus (Streptococcus sobrinus) are main cariogenic bacteria, and the dental caries is caused by the demineralization of the teeth due to the decomposition of the sugar and the production of acid by secreting glucosyltransferase; the proinflammatory factors secreted by Porphyromonas gingivalis (Porphyromonas) and Prevotella intermedia (Prevotella intermedia) cause periodontitis; fusobacterium nucleatum (Fusobacterium nucleatum) and Mueller bacterium (Solobacterium moorei) induce to generate volatile sulfide to cause halitosis, and the bad smelling bacteria such as Fusobacterium nucleatum and Porphyromonas gingivalis are abundantly present in periodontal pocket of patient to metabolize residual cysteine in oral cavity to generate volatile sulfide. Candida albicans (Canidiaalbicas) can induce oral lichen planus disease. The health condition of the oral cavity directly influences the health level of the organism, and diseases such as oral inflammation, gingival bleeding, ulcer and the like can interfere the function of the immune system of the organism, further influence the flora of the whole digestive system and induce the systemic pathological changes. Therefore, inhibiting the growth and reproduction of oral bacteria and maintaining the balance of oral microorganisms are important measures for preventing and treating oral diseases.

The existing broad-spectrum antibiotics and chemical synthetic drugs can effectively kill pathogenic bacteria in the oral cavity, but can also damage the micro-ecology in the oral cavity to different degrees, so as to cause the problems of dysbacteriosis, ulcer and the like. Aiming at the problems, in order to reduce the dosage and side effects of medical drugs and avoid oral superinfection caused by excessive consumption of antibiotics, the invention aims to provide natural, healthy, green and safe antibacterial toothpaste, which can effectively inhibit the growth of common oral pathogenic bacteria such as porphyromonas gingivalis, modified streptococcus, candida albicans, helicobacter pylori, staphylococcus aureus, escherichia coli and the like, can remove part of odor-causing bacteria and residual odor-causing substrates in the oral cavity, inhibit the growth of pathogenic bacteria such as cariogenic bacteria, periodontal pathogenic bacteria and oral lichen planus, solve the barriers of the prior art, improve common oral problems and promote oral health on the premise of not damaging oral flora.

Disclosure of Invention

In order to solve the problems, the invention provides an antibacterial toothpaste containing triterpene from bamboo shavings, which comprises the following components in parts by weight: 0.01-1 part of bamboo shavings triterpene, 0.1-10 parts of solubilizer, 10-40 parts of friction agent, 1-20 parts of pyrophosphate, 0.1-2 parts of thickening agent, 0.1-0.5 part of anticarious agent and 1-10 parts of foaming agent.

As a preferable technical scheme, the triterpene from bamboo shavings is prepared by a carbon dioxide supercritical extraction method, and the content of triterpene compounds in the obtained product is not less than 50%.

The second aspect of the invention provides a pre-dissolving method of the triterpene in bamboo shavings, which adopts a solubilizer to carry out pre-dissolving treatment, wherein the solubilizer is at least one selected from polyglycerol-10 laurate, polyglycerol-10 myristate, polyglycerol-10 stearate, hydrogenated castor oil and polyoxyethylene ether, and preferably the polyglycerol-10 laurate.

As a preferred technical scheme, the pre-dissolving method of the triterpene in bamboo shavings comprises the following steps: mixing 0.1-10 parts of polyglycerol-10 laurate and 0.01-1 part of bamboo shaving triterpene, heating to 50-80 ℃, and dissolving and mixing uniformly until the mixture is transparent.

As a preferable technical scheme, the rubbing agent is hydrated silica, and the hydrated silica is preferably spherical silicon dioxide, and is more preferably spherical silicon dioxide with the particle size of 1-30 μm.

In a preferred embodiment, the pyrophosphate is tetrasodium pyrophosphate and/or tetrapotassium pyrophosphate.

As a preferred technical solution, the thickener is at least one selected from xanthan gum, cellulose gum, carrageenan and carbomer.

As a preferred technical solution, the anticaries agent is at least one selected from sodium fluoride, sodium monofluorophosphate and stannous fluoride, and is preferably sodium fluoride.

As a preferable technical scheme, the foaming agent is selected from at least one of sodium lauryl sulfate, cocamidopropyl betaine, sodium methyl cocoyl taurate and sodium lauroyl sarcosinate.

As a preferable technical scheme, the bacteriostatic toothpaste containing the triterpene from bamboo shavings also comprises 40 to 90 weight parts of humectant, 0.1 to 0.5 weight part of sweetening agent, 0.1 to 1 weight part of preservative and 0.5 to 1.5 weight parts of essence.

The third aspect of the invention provides a preparation method of bacteriostatic toothpaste containing bamboo shaving triterpenes.

As a preferred technical scheme, the bacteriostatic toothpaste containing the triterpene from bamboo shavings is an aqueous toothpaste, and the preparation method of the aqueous toothpaste comprises the following steps:

(1) adding a thickening agent into a humectant, and uniformly stirring and mixing to obtain a mixture 1 for later use;

(2) adding the triterpene of bamboo shavings into a solubilizer, heating to 50-80 ℃, and stirring to mix and dissolve the triterpene of bamboo shavings to obtain a mixture 2 for later use;

(3) under the condition of stirring, adding the mixture 1 into a humectant, and uniformly stirring and mixing to obtain a mixture 3;

(4) adding the mixture 2 into the mixture 3 under the condition of stirring, and uniformly stirring and mixing to obtain a mixture 4;

(5) adding the sweetening agent, the pyrophosphate, the anticaries agent and the preservative into the mixture 4 under the stirring condition, and uniformly stirring and mixing to obtain a mixture 5;

(6) adding a friction agent into the mixture 5 under the condition of stirring, and uniformly stirring and mixing to obtain a mixture 6;

(7) adding the foaming agent and the essence into the mixture 6 under the stirring condition, and uniformly stirring and mixing to obtain the product;

as a preferred technical scheme, the bacteriostatic toothpaste containing the triterpene from bamboo shavings is anhydrous toothpaste, and the preparation method of the anhydrous toothpaste comprises the following steps:

s1, adding the thickening agent into the humectant, and uniformly stirring and mixing to obtain a mixture A;

s2, mixing the solubilizer and the bamboo shavings triterpene, heating to 50-80 ℃ until the solubilizer and the bamboo shavings triterpene are dissolved and mixed uniformly to obtain a mixture B;

s3, heating the rest humectant to 50-80 ℃, adding the mixture A and the mixture B while stirring, continuously stirring for 0.5-2h at 50-80 ℃, uniformly mixing and stirring, and stopping heating to obtain a mixture C;

s4 adding the sweetening agent, the anticaries agent, the preservative and the pyrophosphate into the mixture C under the stirring condition, and uniformly mixing and stirring to obtain a mixture D;

s5, adding the friction agent into the mixture D under the stirring condition, and uniformly mixing and stirring to obtain a mixture E;

and S6, adding the foaming agent and the essence into the mixture E under the stirring condition, and uniformly mixing and stirring to obtain the product.

Has the advantages that: the natural healthy, green and safe botanical drug, namely the triterpene from bamboo shavings, is used as a raw material, and an abrasive, pyrophosphate, a solubilizer and the like in a synergistic system are used to prepare the natural antibacterial toothpaste, so that the prepared antibacterial toothpaste can effectively inhibit the growth of common oral pathogenic bacteria, such as porphyromonas gingivalis, modified streptococcus, candida albicans, helicobacter pylori, staphylococcus aureus, escherichia coli and the like, can remove part of odor-causing bacteria and residual odor-causing substrates in the oral cavity, and can inhibit the growth of pathogenic bacteria, such as cariogenic bacteria, periodontal pathogenic bacteria and oral lichen planus. The bacteriostatic toothpaste containing the triterpene from bamboo shavings has the advantages that the special type of abrasive agent hydrated silica and tetrasodium pyrophosphate are added, and the biological membrane on the surface of the tooth is removed through mechanical friction and desorption. The two have synergistic effect, solve the barriers of the prior art, improve common oral problems on the premise of not destroying oral flora, refresh breath and improve oral health. In addition, the invention solves the problem of the solubility of the triterpene in bamboo shavings by adding the polyglycerol fatty acid component, so that the triterpene in bamboo shavings can be quickly and effectively applied to the water-containing toothpaste and the anhydrous toothpaste.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:

FIG. 1 is a graph showing the bacteriostatic effect of the bacteriostatic toothpaste containing triterpene from bamboo shavings on Porphyromonas gingivalis prepared in example 1.

In the figure: 1a shows that the bacteriostatic toothpaste containing the triterpene from caulis bambusae in taeniam prepared in example 1 is not added, and 1b shows that the bacteriostatic toothpaste containing the triterpene from caulis bambusae in taeniam prepared in example 1 is added.

Fig. 2 is a graph of the bacteriostatic effect of the bacteriostatic toothpaste containing the triterpene from bamboo shavings prepared in example 1 on candida albicans.

In the figure: 2a shows that the bacteriostatic toothpaste containing the triterpene from caulis bambusae in taeniam prepared in example 1 is not added, and 2b shows that the bacteriostatic toothpaste containing the triterpene from caulis bambusae in taeniam prepared in example 1 is added.

Fig. 3 is a graph of the bacteriostatic effect of the bacteriostatic toothpaste containing the triterpene from bamboo shavings prepared in example 1 on streptococcus mutans.

In the figure: 3a shows that the bacteriostatic toothpaste containing the triterpene from caulis bambusae in taeniam prepared in example 1 is not added, and 3b shows that the bacteriostatic toothpaste containing the triterpene from caulis bambusae in taeniam prepared in example 1 is added.

Fig. 4 is a graph of the bacteriostatic effect of the bacteriostatic toothpaste containing the triterpene from bamboo shavings prepared in example 1 on staphylococcus aureus.

In the figure: 4a shows that the bacteriostatic toothpaste containing the triterpene from caulis bambusae in taeniam prepared in example 1 is not added, and 4b shows that the bacteriostatic toothpaste containing the triterpene from caulis bambusae in taeniam prepared in example 1 is added.

FIG. 5 is a graph showing the bacteriostatic effect of the bacteriostatic toothpaste containing triterpene from bamboo shavings on Escherichia coli prepared in example 1.

In the figure: 5a shows that the bacteriostatic toothpaste containing the triterpene from caulis bambusae in taeniam prepared in example 1 is not added, and 5b shows that the bacteriostatic toothpaste containing the triterpene from caulis bambusae in taeniam prepared in example 1 is added.

FIG. 6 is a graph showing the bacteriostatic effect of the bacteriostatic toothpaste containing triterpene from bamboo shavings on helicobacter pylori prepared in example 1.

In the figure: 6a shows that the bacteriostatic toothpaste containing the triterpene from caulis bambusae in taeniam prepared in example 1 is not added, and 6b shows that the bacteriostatic toothpaste containing the triterpene from caulis bambusae in taeniam prepared in example 1 is added.

Detailed Description

The technical features in the technical solutions provided by the present invention are further clearly and completely described below with reference to the specific embodiments, but the scope of protection of the present invention is not limited thereto.

"preferred", "more preferred", and the like, in the present invention, refer to embodiments of the invention that may provide certain benefits, under certain circumstances. However, other embodiments may be preferred, under the same or other circumstances. Furthermore, the recitation of one or more preferred embodiments does not imply that other embodiments are not useful, nor is it intended to exclude other embodiments from the scope of the invention.

The invention provides bacteriostatic toothpaste containing bamboo shaving triterpenes, which comprises the following components in parts by weight: 0.01-1 part of bamboo shavings triterpene, 0.1-10 parts of solubilizer, 10-40 parts of friction agent, 1-20 parts of pyrophosphate, 0.1-2 parts of thickening agent, 0.1-0.5 part of anticarious agent and 1-10 parts of foaming agent.

The bacteriostatic toothpaste containing the triterpene from bamboo shavings comprises water-containing toothpaste and anhydrous toothpaste.

Triterpene of bamboo shavings

Bamboo shavings are dried middle layer of stems of green-stem bamboo bambusatus Munro, bamboo grandis Sinocala leucolytica (Munro) Mc-pure var. pubescens P.F. L i or bamboo Phyllostachys nigra (L odd.) Munro var. hennis (Mitf.) Stapf Rendle, sweet and slightly cold, meridian tropism, lung and stomach meridians, main functional indications, heat clearing and phlegm resolving, vexation relieving and vomiting stopping, phlegm and phlegm heat cough, gallbladder fire phlegm, dysphoria vomiting, palpitation losing eyes, stroke phlegm, glossolalia, stomach heat vomiting, pregnancy vomiting, threateness, bamboo of light bamboo contains cAMP phosphodiesterase inhibiting component, 2, 5-dimethoxy-para-hydroxybenzaldehyde (p-hydroxy benzaldehyde), syringaldehyde (2 '-2-dihydroxy benzaldehyde), and syringaldehyde (2' -2-ethyl-2-dihydroxy-2-ethyl-dimethyl benzoate).

The applicant finds that the toothpaste prepared by using the bamboo shaving triterpene as the raw material has excellent antibacterial and bacteriostatic properties, which is probably because the bamboo shaving triterpene can inhibit oral pathogenic bacteria from adhering to oral cells and biomembranes, inhibit the activity of bacteria acid production and acid production enzyme, inhibit the proinflammatory and related gene expression of bacteria, inhibit the activity of bacteria odor production and odor production enzyme, destroy the bacterial structure to cause the leakage of cell contents, thereby achieving the antibacterial and bacteriostatic effects.

In a preferred embodiment, the triterpene from bamboo shavings is prepared by a carbon dioxide supercritical extraction method, and the content of triterpene compounds in the obtained product is not less than 50%.

The triterpene of bamboo shavings is originated from the college of food engineering of Zhejiang university, and has the following model: triterpene of bamboo shavings.

Solubilizer

The second aspect of the invention provides a method for pre-dissolving triterpene in bamboo shavings, which adopts solubilizer to carry out pre-dissolving treatment.

In a preferred embodiment, the solubilizer is selected from at least one of polyglycerol-10 laurate, polyglycerol-10 myristate, polyglycerol-10 stearate, hydrogenated castor oil, polyoxyethylene ether.

In a more preferred embodiment, the solubilizing agent is polyglycerol-10 laurate.

In a preferred embodiment, the method for pre-dissolving the triterpene from bamboo shavings comprises the following steps: mixing 0.1-10 parts of polyglycerol-10 laurate and 0.01-1 part of bamboo shaving triterpene, heating to 50-80 ℃, and dissolving and mixing uniformly until the mixture is transparent.

In a more preferred embodiment, the method for pre-dissolving the triterpene from bamboo shavings comprises the following steps: mixing 1 part of polyglycerol-10 laurate and 0.1 part of bamboo-shaving triterpene, heating to 60 ℃, and dissolving and mixing uniformly until the mixture is transparent.

In a preferred embodiment, the weight ratio of the triterpene from bamboo shavings to the polyglycerol-10 laurate is 1: (5-15).

In a more preferred embodiment, the weight ratio of the triterpene from bamboo shavings to the polyglycerol-10 laurate is 1: 10.

the triterpene belongs to terpenoid components, the solubility of which is poor, and the applicant unexpectedly finds that when the triterpene is pretreated by taking polyglycerol-10 laurate as a solubilizer, the weight ratio of the triterpene to the polyglycerol-10 laurate is limited to 1: (5-15), the stability of the triterpene in the bamboo shavings in the toothpaste can be improved while the better solubility is ensured. The anti-bacteria toothpaste is characterized in that under specific conditions, the triterpene of bamboo shavings and the polyglycerol-10 laurate are interacted, and a certain steric hindrance effect is added, so that the triterpene of bamboo shavings can be quickly and effectively used in toothpaste, does not settle, can be uniformly distributed in the toothpaste, is used for coordinating humectant, anticariogenic agent, abrasive agent and the like in a system, all the components supplement each other and have better compatibility, thereby achieving the best antibacterial effect and effectively inhibiting the formation of six common oral pathogenic bacteria, namely escherichia coli, staphylococcus aureus, porphyromonas gingivalis, streptococcus mutans, candida albicans and helicobacter pylori. Hydrogenated castor oil is adopted as a solubilizer, and the triterpene in bamboo shavings can be settled, so that the antibacterial and bacteriostatic effects are influenced.

Friction agent

In a preferred embodiment, the abrasive is hydrated silica.

In a more preferred embodiment, the hydrated silica is spherical silicon dioxide having a particle size of 1 to 30 μm.

In a more preferred embodiment, the hydrated silica is spherical silicon dioxide having a particle size of 20 μm.

Spherical silicon dioxide with the particle size of 1-30 mu m is used as an abrasive, biological membranes on the surfaces of teeth can be removed through physical friction and dissociation, and bamboo shaving triterpenes, tetrasodium pyrophosphate and the like in a synergistic system can continuously act, improve the health state of oral cavity and reduce the occurrence of common oral diseases.

Pyrophosphate salt

The pyrophosphate belongs to a calcium phosphate surface active construct, can promote the original membrane protein containing pigment components on the tooth surface to release the adsorption effect, decompose and soften color spots and bring the whitening effect. It can also eliminate magnesium ion and calcium ion deposited on teeth by chelation.

Thickening agent

In a preferred embodiment, the thickener is selected from at least one of xanthan gum, cellulose gum, carrageenan, and carbomer.

In a more preferred embodiment, the bacteriostatic toothpaste containing triterpene from bamboo shavings is an aqueous toothpaste, and the thickening agent is xanthan gum and sodium cellulose.

In a more preferred embodiment, the bacteriostatic toothpaste containing the triterpene from bamboo shavings is an aqueous toothpaste, the thickening agent is xanthan gum and sodium cellulose, and the thickening agent contains 0.5 part by weight of xanthan gum and 0.5 part by weight of sodium cellulose.

In a more preferred embodiment, the bacteriostatic toothpaste containing triterpene from bamboo shavings is an anhydrous toothpaste, and the thickening agent is xanthan gum and carbomer.

In a more preferred embodiment, the bacteriostatic toothpaste containing the triterpene from bamboo shavings is an anhydrous toothpaste, the thickening agent is xanthan gum and carbomer, and the thickening agent contains 0.1 part by weight of xanthan gum and 0.9 part by weight of carbomer.

Anticariogenic agent

In a preferred embodiment, the anticaries agent is selected from at least one of sodium fluoride, sodium monofluorophosphate, stannous fluoride.

In a more preferred embodiment, the anticaries agent is sodium fluoride.

Foaming agent

In a preferred embodiment, the foaming agent is selected from at least one of sodium lauryl sulfate, cocamidopropyl betaine, sodium methylcocoyltaurate, sodium lauroyl sarcosinate.

In a more preferred embodiment, the foaming agent is selected from at least one of sodium lauryl sulfate, cocamidopropyl betaine, sodium methylcocoyltaurate.

In a more preferred embodiment, the bacteriostatic toothpaste containing bamboo-shaving triterpene is an aqueous toothpaste, and the foaming agent is sodium lauryl sulfate and cocamidopropyl betaine.

In a more preferred embodiment, the bacteriostatic toothpaste containing the triterpene from bamboo shavings is an anhydrous toothpaste, and the foaming agent is sodium methyl cocoyl taurate or cocamidopropyl betaine.

In a preferred embodiment, the bacteriostatic toothpaste containing the triterpene from bamboo shavings further comprises 40 to 90 parts by weight of humectant, 0.1 to 0.5 part by weight of sweetener, 0.1 to 1 part by weight of preservative and 0.5 to 1.5 parts by weight of essence.

In a more preferable embodiment, the bacteriostatic toothpaste containing the triterpene from bamboo shavings further comprises 60 parts by weight of humectant, 0.3 part by weight of sweetener, 0.5 part by weight of preservative and 1 part by weight of essence.

Moisture-retaining agent

In a preferred embodiment, the humectant is selected from at least one of glycerin, sorbitol, propylene glycol, polyethylene glycol.

In a more preferred embodiment, the bacteriostatic toothpaste containing the triterpene from bamboo shavings is an aqueous toothpaste, and the humectant is glycerin and sorbitol.

In a more preferred embodiment, the bacteriostatic toothpaste containing the triterpene from bamboo shavings is anhydrous toothpaste, and the humectant is glycerin and PEG-8.

Sweetening agent

In a preferred embodiment, the sweetener is selected from at least one of sodium saccharin, xylitol, sucralose.

In a more preferred embodiment, the bacteriostatic toothpaste containing triterpene from bamboo shavings is an aqueous toothpaste, and the sweetener is saccharin sodium.

In a more preferred embodiment, the bacteriostatic toothpaste containing triterpene from bamboo shavings is an anhydrous toothpaste, and the sweetener is sucralose.

Preservative

In a preferred embodiment, the preservative is selected from at least one of sodium benzoate, methylparaben, propylparaben.

In a more preferred embodiment, the preservative is sodium benzoate.

Essence

Essence refers to a component that imparts fragrance to a product.

The perfume of the present invention is not particularly limited and may be obtained commercially, for example, commercially available perfumes include, but are not limited to, those available from Tianjin Xinnuson Biotechnology Ltd.

In a preferred embodiment, the bacteriostatic toothpaste containing the triterpene from bamboo shavings is an aqueous toothpaste, and the preparation method of the aqueous toothpaste comprises the following steps:

(7) adding the foaming agent and the essence into the mixture 6 under the condition of stirring, and stirring and mixing uniformly to obtain the product.

In a more preferred embodiment, the bacteriostatic toothpaste containing triterpene from bamboo shavings is an aqueous toothpaste, and the preparation method of the aqueous toothpaste comprises the following steps:

(1) adding a thickening agent into glycerol, and uniformly stirring and mixing to obtain a mixture 1 for later use;

(2) adding the triterpene of bamboo shavings into a solubilizer, heating to 60 ℃, and stirring to mix and dissolve the triterpene of bamboo shavings to obtain a mixture 2 for later use;

(3) adding the mixture 1 into sorbitol under stirring, and uniformly stirring and mixing to obtain a mixture 3;

In a preferred embodiment, the bacteriostatic toothpaste containing the triterpene from bamboo shavings is an anhydrous toothpaste, and the preparation method of the anhydrous toothpaste comprises the following steps:

In a more preferred embodiment, the bacteriostatic toothpaste containing the triterpene from bamboo shavings is an anhydrous toothpaste, and the preparation method of the anhydrous toothpaste comprises the following steps:

s1, adding the thickening agent into 5 parts of glycerol and 5 parts of PEG-8, and uniformly stirring and mixing to obtain a mixture A;

s2, mixing the solubilizer and the bamboo shavings triterpene, heating to 60 ℃ until the solubilizer and the bamboo shavings triterpene are dissolved and mixed uniformly to obtain a mixture B;

s3, heating the residual glycerol to 60 ℃, adding the mixture A and the mixture B while stirring, continuously stirring for 1h at 60 ℃, uniformly mixing and stirring, and stopping heating to obtain a mixture C;

Hereinafter, the present invention will be described in more detail by way of examples, but it should be understood that these examples are merely illustrative and not restrictive. In addition, all the raw materials are commercially available if not particularly limited.

Examples

Example 1

The embodiment 1 of the invention provides bacteriostatic toothpaste containing triterpene from bamboo shavings, which comprises the following components in parts by weight: 0.1 part of bamboo-shaving triterpene, 1 part of solubilizer, 20 parts of abrasive, 12.86 parts of pyrophosphate, 1 part of thickener, 0.24 part of anticarious agent, 3 parts of foaming agent, 60 parts of humectant, 0.3 part of sweetener, 0.5 part of preservative and 1 part of essence.

The solubilizer is polyglycerol-10 laurate.

The abrasive is hydrated silica.

The hydrated silica is spherical silicon dioxide and has a particle size of 20 μm.

The pyrophosphate is tetrasodium pyrophosphate.

The thickening agent is xanthan gum and sodium cellulose.

The thickening agent contains 0.5 weight part of xanthan gum and 0.5 weight part of sodium cellulose.

The anticariogenic agent is sodium fluoride.

The foaming agent is sodium lauryl sulfate and cocamidopropyl betaine.

The foaming agent contains 2 parts by weight of sodium lauryl sulfate and 1 part by weight of cocamidopropyl betaine.

The humectant is glycerol and sorbitol.

The humectant contains 5 parts by weight of glycerin and 55 parts by weight of sorbitol.

The sweetener is saccharin sodium.

The preservative is sodium benzoate.

The embodiment 1 of the invention also provides a preparation method of the bacteriostatic toothpaste containing the triterpene from bamboo shavings, which comprises the following steps:

Example 2

The embodiment 2 of the invention provides bacteriostatic toothpaste containing triterpene from bamboo shavings, which comprises the following components in parts by weight: 0.01 part of bamboo-shaving triterpene, 1 part of solubilizer, 20 parts of abrasive, 12.86 parts of pyrophosphate, 1 part of thickener, 0.24 part of anticarious agent, 3 parts of foaming agent, 60 parts of humectant, 0.3 part of sweetener, 0.5 part of preservative and 1 part of essence.

The solubilizer is polyglycerol-10 laurate.

The abrasive is hydrated silica.

The pyrophosphate is tetrasodium pyrophosphate.

The thickening agent is xanthan gum and carbomer.

The thickening agent contains 0.1 part by weight of xanthan gum and 0.9 part by weight of carbomer.

The anticariogenic agent is sodium fluoride.

The foaming agent is sodium methyl cocoyl taurate and cocamidopropyl betaine.

The foaming agent contains 1 weight part of sodium methyl cocoyl taurate and 2 weight parts of cocamidopropyl betaine.

The humectant is glycerol and PEG-8.

The humectant contains 55 parts by weight of glycerin and 5 parts by weight of PEG-8.

The sweetener is sucralose.

The preservative is sodium benzoate.

The embodiment 2 of the invention also provides a preparation method of the bacteriostatic toothpaste containing the triterpene from bamboo shavings, which comprises the following steps:

s3, heating 50 parts of glycerol to 60 ℃, adding the mixture A and the mixture B while stirring, continuously stirring for 1 hour at 60 ℃, uniformly mixing and stirring, and stopping heating to obtain a mixture C;

Example 3

The embodiment 3 of the invention provides bacteriostatic toothpaste containing triterpene from bamboo shavings and a preparation method of the bacteriostatic toothpaste containing triterpene from bamboo shavings, and the specific implementation mode is the same as that of the embodiment 1, except that the content of triterpene from bamboo shavings is replaced by 0 part.

Example 4

The embodiment 4 of the invention provides bacteriostatic toothpaste containing triterpene from bamboo shavings and a preparation method of the bacteriostatic toothpaste containing triterpene from bamboo shavings, and the specific implementation mode is the same as that of the embodiment 1, except that the content of the solubilizer is replaced by 0 part.

Example 5

The embodiment 5 of the invention provides bacteriostatic toothpaste containing triterpene from bamboo shavings and a preparation method of the bacteriostatic toothpaste containing triterpene from bamboo shavings, and the specific implementation mode is the same as that of the embodiment 1, except that the content of the solubilizer is replaced by 0.5 part.

Example 6

The embodiment 6 of the invention provides bacteriostatic toothpaste containing triterpene from bamboo shavings and a preparation method of the bacteriostatic toothpaste containing triterpene from bamboo shavings, and the specific implementation mode is the same as that of the embodiment 1, except that the solubilizer is replaced by PEG-60 hydrogenated castor oil, and the abrasive is replaced by calcium carbonate.

Example 7

The embodiment 7 of the invention provides bacteriostatic toothpaste containing triterpene from bamboo shavings and a preparation method of the bacteriostatic toothpaste containing triterpene from bamboo shavings, and the specific implementation mode is the same as that of the embodiment 1, except that the content of the abrasive is replaced by 0 part.

Performance evaluation

1. Evaluation of antibacterial and bacteriostatic effects

The antibacterial toothpaste containing the triterpene from bamboo shavings prepared in examples 1 to 7 was tested for antibacterial and bacteriostatic effects on escherichia coli, staphylococcus aureus, porphyromonas gingivalis, streptococcus mutans, candida albicans and helicobacter pylori according to QB/T2738-.

The results are shown in Table 1.

TABLE 1 antibacterial and bacteriostatic property test

Wherein A represents helicobacter pylori, B represents Escherichia coli, C represents Staphylococcus aureus, D represents Porphyromonas gingivalis, E represents Streptococcus mutans, and F represents Candida albicans. Helicobacter pylori is a suspected pathogenic bacterium of halitosis, escherichia coli and staphylococcus aureus are suspected pathogenic bacteria related to pyogenic inflammation, porphyromonas gingivalis is a suspected pathogenic bacterium of periodontal inflammation and halitosis, streptococcus mutans is a pathogenic bacterium related to dental caries, and candida albicans is a suspected pathogenic fungus of dental ulcer.

2. Toothpaste friction value safety test

The friction value safety of the bacteriostatic toothpaste containing the bamboo shaving triterpenes prepared in the examples 1-7 is tested according to GB/T35832 and 2018, and the experimental results are shown in Table 2.

TABLE 2 Friction value safety test results

3. Evaluation of Using Effect

Screening 40 persons, with the age distribution between 20-40 years, wherein 20 persons were born 20, and the subjects all had some degree of oral health problems. The bacteriostatic toothpaste containing the triterpene from caulis Bambusae in Taenia prepared in examples 1-7 was used for 1 month.

The results are shown in Table 3.

Table 3 results of using the bacteriostatic toothpastes containing triterpene from bamboo shavings prepared in examples 1 to 7

According to experimental results, the bacteriostatic toothpaste containing the bamboo shaving triterpenes, which is prepared by the invention, has an obvious antibacterial and bacteriostatic effect, can well inhibit common oral pathogenic bacteria such as escherichia coli, staphylococcus aureus, porphyromonas gingivalis, streptococcus mutans, candida albicans, helicobacter pylori and the like, can inhibit the development of oral inflammation, can effectively treat oral problems such as halitosis, redness and swelling of gum, bleeding and oral ulcer, can maintain oral health, and is suitable for long-term use.

The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as is contemplated, and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. The use of some numerical ranges in the claims also includes sub-ranges within their range, and variations in these ranges are also to be construed as being covered by the appended claims where possible.

Claims

1. An antibacterial toothpaste containing triterpene from caulis bambusae in taeniam is characterized by comprising the following components in parts by weight: 0.01-1 part of bamboo shavings triterpene, 0.1-10 parts of solubilizer, 10-40 parts of friction agent, 1-20 parts of pyrophosphate, 0.1-2 parts of thickening agent, 0.1-0.5 part of anticarious agent and 1-10 parts of foaming agent.

2. The bacteriostatic toothpaste containing triterpene from bamboo shavings according to claim 1, wherein triterpene from bamboo shavings is prepared by supercritical carbon dioxide extraction, and the content of triterpene compounds in the obtained product is not less than 50%.

3. The bacteriostatic toothpaste containing triterpene from bamboo shavings according to claim 1, wherein the triterpene from bamboo shavings is subjected to pre-dissolution treatment by using a solubilizer selected from at least one of polyglycerol-10 laurate, polyglycerol-10 myristate, polyglycerol-10 stearate, hydrogenated castor oil and polyoxyethylene ether, preferably polyglycerol-10 laurate.

4. Bacteriostatic toothpaste containing triterpene from bamboo shavings according to claim 1, wherein the abrasive is hydrated silica, preferably spherical silica, more preferably spherical silica having a particle size of 1-30 μm.

5. The bacteriostatic toothpaste containing triterpene from bamboo shavings according to claim 1, wherein the pyrophosphate is tetrasodium pyrophosphate and/or tetrapotassium pyrophosphate.

6. The bacteriostatic toothpaste containing triterpene from bamboo shavings according to claim 1, wherein the thickener is at least one selected from xanthan gum, cellulose gum, carrageenan and carbomer.

7. Bacteriostatic toothpaste containing triterpene from bamboo shavings according to claim 1, wherein said anticaries agent is selected from at least one of sodium fluoride, sodium monofluorophosphate and stannous fluoride, preferably sodium fluoride.

8. Bacteriostatic toothpaste containing triterpene from bamboo shavings according to claim 1, wherein said foaming agent is selected from at least one of sodium lauryl sulfate, cocamidopropyl betaine, sodium methylcocoyl taurate, sodium lauroyl sarcosinate.

9. The bacteriostatic toothpaste containing the triterpene from bamboo shavings according to claims 1 to 8, wherein the bacteriostatic toothpaste containing the triterpene from bamboo shavings further comprises 40 to 90 parts by weight of humectant, 0.1 to 0.5 part by weight of sweetener, 0.1 to 1 part by weight of preservative and 0.5 to 1.5 parts by weight of essence.

10. A method for preparing bacteriostatic toothpaste containing triterpene from bamboo shavings according to claim 9, wherein the bacteriostatic toothpaste containing triterpene from bamboo shavings comprises aqueous toothpaste and anhydrous toothpaste;

the preparation method of the water-containing toothpaste comprises the following steps:

the preparation method of the anhydrous toothpaste comprises the following steps: