CN111378575B - Metarrhizium anisopliae culture device - Google Patents
Metarrhizium anisopliae culture device Download PDFInfo
- Publication number
- CN111378575B CN111378575B CN202010358284.7A CN202010358284A CN111378575B CN 111378575 B CN111378575 B CN 111378575B CN 202010358284 A CN202010358284 A CN 202010358284A CN 111378575 B CN111378575 B CN 111378575B
- Authority
- CN
- China
- Prior art keywords
- culture
- air
- fixedly connected
- autoclave
- pipe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/14—Drying
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Computer Hardware Design (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The metarhizium anisopliae culture device comprises an autoclave, a ferrying vehicle, a plurality of sections of steam curing vehicles, a culture plate placing rack, a spraying part and a ventilation part; culture dish rack: the device comprises a bracket fixedly connected to each steam-curing vehicle, wherein a plurality of layers of mounting frames are sequentially arranged on the bracket at intervals from top to bottom, and a plurality of culture plates are arranged in each layer of mounting frames; spraying part: the device comprises a spray branch pipe arranged above each culture disc and a plurality of spray header pipes which are respectively and fixedly connected in autoclaves at one side of each layer of mounting rack; ventilation part: the device comprises an axial flow fan, an air inlet pipe and an exhaust pipe which are respectively communicated with two ends of the autoclave, wherein the fan is arranged on the air inlet pipe, an air suction port of the fan is communicated with an air filter and an air sterilizer through an air suction pipe, and an air heater is arranged on the air inlet pipe. The method is used for solving the problems that the existing metarhizium anisopliae culture is easy to mix with mixed bacteria, the production efficiency is low and the labor intensity of workers is high.
Description
Technical Field
The invention relates to a metarhizium anisopliae culture device.
Background
The metarhizium anisopliae is broad-spectrum entomopathogenic bacteria, and the metarhizium anisopliae host insects are more than 200 according to statistics, and can parasitize the tortoise shell, the weevil, the golden needle worms, the lepidoptera pest larvae, the hemiptera stink bugs and the like. The insect can be induced to produce metarhizium anisopliae, and repeated infection can be formed in the population. In application, the metarhizium anisopliae is mainly used for controlling pests, and the metarhizium anisopliae is developed into a fungus pesticide which is inferior to the beauveria bassiana in terms of control scale. The metarhizium anisopliae is harmless to human and livestock, safe to natural enemy insects and free from environmental pollution. The culture process of the metarhizium anisopliae generally adopts a plastic bag method for culture, and comprises the steps of sterilizing a solid culture medium, cooling, inoculating, bagging, ventilating, airing, culturing and the like, wherein the culture process needs to repeatedly contact the culture medium with outside air, so that the mixed bacteria are easy to mix into the mixed bacteria to pollute the culture medium, and the culture chamber, the plastic bag, the culture disc and the like all need to be manually disinfected, so that the production efficiency is influenced, and the labor intensity of workers is higher.
Disclosure of Invention
The invention aims to solve the technical problems of easiness in mixing mixed bacteria, low production efficiency and high labor intensity of workers in the conventional metarhizium anisopliae culture device.
In order to solve the problems, the technical scheme to be solved by the invention is as follows:
the metarhizium anisopliae culture device comprises an autoclave, a ferrying vehicle, a plurality of sections of steam curing vehicles, a culture plate placing rack, a spraying part and a ventilation part;
culture dish rack: the device comprises a bracket fixedly connected to each steam-curing vehicle, wherein a plurality of layers of mounting frames are sequentially arranged on the bracket at intervals from top to bottom, and a plurality of culture plates are arranged in each layer of mounting frames;
spraying part: the device comprises spray branch pipes arranged above each culture dish, a plurality of spray header pipes respectively fixedly connected in an autoclave at one side of each layer of mounting frame, a suspension liquid storage tank and a water separator, wherein the suspension liquid storage tank and the water separator are arranged outside the autoclave;
ventilation part: the device comprises an axial flow fan, an air inlet pipe and an exhaust pipe which are respectively communicated with two ends of the autoclave, wherein the fan is arranged on the air inlet pipe, an air suction port of the fan is communicated with an air filter and an air sterilizer through an air suction pipe, and an air heater is arranged on the air inlet pipe.
The multi-layer mounting frames are sequentially arranged at two sides of the support from top to bottom at intervals, a plurality of culture plates are arranged in each layer of mounting frames, the spraying parts are two groups, the two groups of spraying parts respectively correspond to the culture plates at two sides of the support, and the two water separators in the two groups of spraying parts are communicated through a second hose.
Still include steam curing car drive arrangement, steam curing car drive arrangement includes a plurality of axle beds at fixed connection in the cauldron that evaporates, and a plurality of axle beds are equipped with the pivot, install the gear in the pivot, and pivot one end pass behind the cauldron that evaporates with fixed gear motor transmission that sets up and be connected, install the rack with gear engaged with in steam curing car bottom surface.
Still include control system, control system includes PLC, the travel switch of setting in pivot one side, a plurality of first solenoid valve of installing respectively on each feed liquor pipe, install the second solenoid valve on the blast pipe, install humidity transducer and temperature sensor on evaporating kettle, carousel on the rotation axis, fixedly connected with is used for triggering travel switch's the piece that bumps on the carousel, humidity transducer, temperature sensor and travel switch connect the PLC input, PLC control water pump, gear motor, air sterilizer, air heater, each first solenoid valve and second solenoid valve.
The spray nozzle comprises a sleeve fixedly connected to the spray branch pipe, the lower end of the sleeve is fixedly connected with a ring cover, a shaft rod is arranged in the ring cover, the shaft rod is fixedly connected with a piston arranged in the sleeve, a plurality of first drain holes are formed in the piston, a plurality of second drain holes are formed in the lower end of the sleeve, the piston descends to the first drain holes and are communicated with the second drain holes, a spring is sleeved outside the shaft rod, and two ends of the spring respectively abut against the piston and the ring cover.
The lower end of the sleeve is fixedly connected with a housing, and each second drain hole is arranged in the housing.
The cultivation dish includes peripheral frame, frame bottom fixed connection filter screen.
The beneficial effects of the invention are as follows: the method effectively prevents the pollution in the culture process, simplifies the production process, improves the production efficiency, simultaneously can lead the destruxins of Metarrhizium anisopliae to be less illuminated and improves the spore yield of the destruxins of Metarrhizium anisopliae by sterilizing, inoculating and culturing the destruxins of Metarrhizium anisopliae culture medium in a concentrated autoclave.
Drawings
The invention is further described with reference to the accompanying drawings:
figure 1 is a schematic view of a front cross-sectional structure of the present invention,
fig. 2 is a schematic view of a front sectional structure of the present invention, in which a spray portion is not shown,
FIG. 3 is a schematic view of a sectional front view of the present invention, showing a tray rack not shown,
FIG. 4 is a schematic top view of the present invention, showing the culture dish rack,
figure 5 is a schematic cross-sectional view of the structure at A-A in figure 1,
figure 6 is a schematic view of the structure of the nozzle according to the present invention,
figure 7 is a schematic view of the structure of the present invention with respect to the nozzle,
FIG. 8 is a schematic diagram of the connection relationship between electrical components according to the present invention.
In the figure: the ferry vehicle 1, the autoclave 2, the spray part 3, the spray header 31, the spray branch 32, the nozzle 33, the pipe sleeve 331, the shaft 332, the spring 333, the second drain hole 334, the first drain hole 335, the housing 336, the ring cover 337, the water separator 34, the first valve 35, the liquid inlet pipe 36, the suspension tank 37, the water pump 38, the first hose 39, the cultivation tray 4, the filter screen 41, the frame 42, the cultivation vehicle 5, the evaporation vehicle driving device 6, the rotating shaft 61, the gear 62, the rack 63, the gear motor 64, the turntable 65, the ram 66, the travel switch 67, the ventilation part 7, the air heater 71, the air inlet pipe 72, the fan 73, the air sterilizer 74, the air filter 75, the second valve 76, the exhaust pipe 77, the cultivation tray placing frame 8, the bracket 81, the mounting bracket 82, the humidity sensor 9, and the temperature sensor 10.
Detailed Description
As shown in fig. 1 to 8, the metarhizium anisopliae culture device comprises an autoclave 2, a ferry vehicle 1, a multi-section steam curing vehicle 5, a culture dish placing rack 8, a spraying part 3 and a ventilation part 7;
culture dish rack 8: the device comprises a bracket 81 fixedly connected to each steam-curing vehicle 5, wherein a plurality of layers of mounting frames 82 are sequentially arranged on the bracket 81 at intervals from top to bottom, and a plurality of culture trays 4 are arranged in each layer of mounting frames 82;
spray part 3: the device comprises spray branch pipes 32 arranged above each culture dish 4, a plurality of spray header pipes 31 respectively fixedly connected in an autoclave 2 at one side of each layer of mounting frame 82, a suspension storage tank 37 and a water separator 34 which are arranged outside the autoclave 2, wherein each spray header pipe 31 is respectively communicated with the water separator 34 through a liquid inlet pipe 36, the suspension storage tank 37 is communicated with the water separator 34 through a first hose 39, a water pump 38 is arranged on the first hose 39, a first valve 35 is arranged on the liquid inlet pipe 36, a plurality of spray nozzles 33 are arranged on the spray branch pipes 32, and when each culture dish 4 on an autoclaved vehicle enters the autoclave 2, the spray branch pipes 32 and the spray nozzles 33 cannot collide with the culture dish 4 and the culture dish placing frame 8;
ventilation section 7: the device comprises an axial flow fan 73, an air inlet pipe 72 and an air outlet pipe 77 which are respectively communicated with two ends of the autoclave 2, wherein a second valve 76 is arranged on the air outlet pipe 77, the fan 73 is arranged on the air inlet pipe 72, an air suction port of the fan 73 is communicated with an air filter 75 and an air sterilizer 74 through an air suction pipe, and an air heater 71 is arranged on the air inlet pipe 72; the air filter 75 filters out dust in the air, and foreign bacteria in the outside air are killed by the air sterilizer 74, wherein the air filter 75 is a plate-frame air filter 75, the air sterilizer 74 can be an 'Lian Da brand pipeline plug-in ultraviolet C air sterilizer 74', and the bacterial purification rate is over 99.96%.
The application method of the invention comprises the following steps:
the first step: after the prepared solid culture medium is paved in each culture disc 4, the culture discs 4 are sent into the autoclave 2 by the steam curing vehicle 5, and then the two side kettle doors are closed.
And a second step of: steam at 115℃was fed into autoclave 2 and the solid medium was sterilized with steam for 70 minutes.
And a third step of: the fan 73 and the air sterilizer 74 are started, and external cold air is sent into the autoclave 2, so that the temperature of the solid culture medium is reduced to below 30 ℃ or the solid culture medium is waited for to cool naturally.
Fourth step: the metarhizium anisopliae inoculation suspension is poured into the suspension storage tank 37, then the water pump 38 is started, and the inoculation suspension is sprayed to the solid medium, so that the metarhizium anisopliae inoculation is completed.
Fifth step: after the metarhizium anisopliae is inoculated, a fan 73 and an air sterilizer 744 are started every 1 hour, and sterilized air is sent into the autoclave 2, so that the autoclave 2 is kept to be ventilated for 8-10 days.
Sixth step: the air heater 71 was turned on, and the air was heated to about 35℃and then fed into the autoclave 2, and dried with hot air for 36 hours, to thereby complete the drying of Metarrhizium anisopliae.
Seventh step: and opening kettle doors at two ends of the autoclave 2, and pulling the culture dish 4 out of the autoclave 2 by the steam curing vehicle 5, wherein green spores in the culture dish 4 are green muscardine fungus crude products.
Through the steps, the whole process of the culture of the metarhizium anisopliae is completed in the autoclave 2, and the air fed into the metarhizium anisopliae is fed after being disinfected, so that the pollution of the metarhizium anisopliae to external sundry fungus can be greatly reduced, meanwhile, the whole culture process of the metarhizium anisopliae can not be illuminated, and the spore yield of the metarhizium anisopliae is improved.
The multi-layer installation racks 82 are sequentially arranged on two sides of the support 81 from top to bottom at intervals, a plurality of culture trays 4 are arranged in each layer of installation rack 82, the two groups of spraying parts 3 are respectively corresponding to the culture trays 4 on two sides of the support 81, and two water distributors 34 in the two groups of spraying parts 3 are communicated through a second hose. The design of the culture dish placement frame 8 with the structure is more reasonable, and the weight balance of the two sides of the bracket 81 is kept.
Still include steam curing car drive arrangement 6, steam curing car 5 drive arrangement includes a plurality of axle beds at fixed connection in autoclave 2, installs pivot 61 in a plurality of axle beds, installs gear 62 on the pivot 61, and pivot 61 one end passes behind the autoclave 2 with fixed gear motor 64 transmission connection that sets up, install rack 63 with gear 62 engaged with in steam curing car 5 bottom surface.
When the nozzle 33 sprays the inoculation suspension onto the solid medium, the gear motor 64 can rotate in a positive and negative alternating manner, so that the culture disc 4 is driven to move back and forth through the gear 62 and the gear 62, and the inoculation suspension is uniformly sprayed onto the medium, so that the metarhizium anisopliae can be uniformly cultured.
The automatic control device is characterized by further comprising a control system, wherein the control system comprises a PLC, a travel switch 67 arranged on one side of the rotating shaft 61, a plurality of first electromagnetic valves respectively arranged on each liquid inlet pipe 36, a second electromagnetic valve arranged on the exhaust pipe 77, a humidity sensor 9 and a temperature sensor 10 arranged on the autoclave 2, a turntable 65 arranged on the rotating shaft 61, a collision block 66 for triggering the travel switch 67, wherein the humidity sensor 9, the temperature sensor 10 and the travel switch 67 are fixedly connected with the PLC input end, and the PLC controls the water pump 38, the speed reducing motor 64, the air sterilizer 74, the air heater 71, each first electromagnetic valve and the second electromagnetic valve. The first valve 35 and the second valve 76 described above may be replaced with a first solenoid valve and a second solenoid valve.
After the solid-state culture is disinfected and cooled, the metarhizium anisopliae is cultured by a control system, and the implementation process is as follows:
the first step: the PLC controls the gear motor 64 to start, and whenever the collision block 66 collides with the travel switch 67 twice, the PLC controls the gear motor 64 to rotate in a reversing manner, so that the reciprocating motion of the culture medium is realized, and simultaneously, the water pump 38 is controlled to start and each first electromagnetic valve is controlled to be sequentially opened for 20 seconds and then closed, so that the inoculation suspension is uniformly sprayed on the culture medium.
And a second step of: after the green muscardine fungus inoculation is finished, the PLC controls the fan 73, the air sterilizer 74 and the second electromagnetic valve to be opened for 4 minutes every 1 hour so as to send sterilized air into the autoclave 2, and the autoclave 2 is kept to be ventilated for 8-10 days.
And a third step of: the PLC controls the opening of the air heater 71, the fan 73 and the air sterilizer 74, and sends air with the temperature of about 35 ℃ into the autoclave 2 to dry the metarhizium anisopliae.
Seventh step: after the green muscardine fungus is dried, opening kettle doors at two ends of the autoclave 2, and pulling the culture dish 4 out of the autoclave 2 by the steam curing vehicle 5, wherein green spores in the culture dish 4 are green muscardine fungus crude products.
Therefore, the automatic control of the culture of the metarhizium anisopliae is realized, the labor intensity of workers is reduced, and the culture quality of metarhizium anisopliae in each batch is kept uniform.
The nozzle 33 comprises a sleeve 331 fixedly connected to the spray branch pipe 32, a ring cover 337 is fixedly connected to the lower end of the sleeve 331, a shaft lever 332 is arranged in the ring cover 337, the shaft lever 332 is fixedly connected with a piston 338 arranged in the sleeve, a plurality of first water draining holes 335 are formed in the piston 338, a plurality of second water draining holes 334 are formed in the lower end of the sleeve 331, the piston 338 descends to the first water draining holes 335 and communicate with the second water draining holes 334, a spring 333 is sleeved outside the shaft lever 332, and two ends of the spring 333 respectively abut against the piston 338 and the ring cover 337.
Compared with the case that the first electromagnetic valves are opened simultaneously when the first electromagnetic valves are closed after 20 seconds in sequence, the spraying pressure of the green muscardine fungus inoculation suspension liquid of the spraying branch pipe 32 can be ensured, the spraying branch pipe 32 green muscardine fungus inoculation suspension liquid pushes the piston 338 to downwards move to the first water drain hole 335 to be communicated with the second water drain hole 334, the green muscardine fungus inoculation suspension liquid passes through the first water drain hole 335 and the second water drain hole 334 to be sprayed to the solid culture medium, the shaft lever 332 is inserted into the solid culture medium, and the shaft lever 332 moves back and forth along with the reciprocating motion of the steam curing vehicle 5, so that the solid culture medium can be turned back and forth, and the green muscardine fungus inoculation suspension liquid is uniformly mixed into the solid culture medium; when the spraying branch pipe 32 loses pressure, for example, the water pump 38 is closed, the first electromagnetic valve is opened, the spring 333 pushes the piston 338 upwards, the shaft lever 332 retracts into the sleeve 331, and the culture dish 4 can freely enter and exit the autoclave 2, so that the shaft lever 332 is prevented from colliding with the culture dish 4.
A housing 336 is fixedly connected to the lower end of the sleeve, and each second drain hole 334 is provided in the housing 336. The housing 336 ensures that the metarhizium seed suspension sprays downward.
The culture dish 4 comprises a peripheral frame 42, and a filter screen 41 is fixedly connected to the bottom of the frame 42. This structure can enhance the ventilation effect of the culture dish 4.
Claims (2)
1. The metarhizium anisopliae culture device comprises an autoclave (2), a ferry vehicle (1) and a multi-section steam curing vehicle (5), and is characterized in that: the culture dish comprises a culture dish rack (8), a spraying part (3) and a ventilation part (7);
culture dish rack (8): comprises a bracket (81) fixedly connected to each steam-curing vehicle (5), wherein a plurality of layers of mounting frames (82) are sequentially arranged on the bracket (81) from top to bottom at intervals, and a plurality of culture trays (4) are arranged in each layer of mounting frames (82);
spray part (3): the device comprises spray branch pipes (32) arranged above all culture trays (4), a plurality of spray header pipes (31) which are respectively and fixedly connected in an autoclave (2) at one side of each layer of mounting frame (82), a suspension liquid storage tank (37) and a water separator (34) which are arranged outside the autoclave (2), wherein each spray header pipe (31) is communicated with the water separator (34) through a liquid inlet pipe (36), the suspension liquid storage tank (37) is communicated with the water separator (34) through a first hose (39), a water pump (38) is arranged on the first hose (39), a first valve (35) is arranged on the liquid inlet pipe (36), and a plurality of nozzles (33) are arranged on each spray branch pipe (32);
ventilation part (7): the device comprises a fan (73), an air inlet pipe (72) and an exhaust pipe (77) which are respectively communicated with two ends of an autoclave (2), wherein a second valve (76) is arranged on the exhaust pipe (77), the fan (73) is arranged on the air inlet pipe (72), an air suction port of the fan (73) is communicated with an air filter (75) and an air sterilizer (74) through an air suction pipe, and an air heater (71) is arranged on the air inlet pipe (72);
the steam curing vehicle driving device (6) is characterized by further comprising a plurality of shaft seats fixedly connected in the steam curing kettle (2), a rotating shaft (61) is assembled in the shaft seats, a gear (62) is arranged on the rotating shaft (61), one end of the rotating shaft (61) penetrates through the steam curing kettle (2) and is in transmission connection with a reduction motor (64) fixedly arranged, and a rack (63) meshed with the gear (62) is arranged on the bottom surface of the steam curing vehicle (5);
the automatic control device is characterized by further comprising a control system, wherein the control system comprises a PLC, a travel switch (67) arranged on one side of the rotating shaft (61), a plurality of first electromagnetic valves respectively arranged on each liquid inlet pipe (36), a second electromagnetic valve arranged on the exhaust pipe (77), a humidity sensor (9) and a temperature sensor (10) arranged on the autoclave (2), a turntable (65) arranged on the rotating shaft (61), a bump (66) for triggering the travel switch (67) is fixedly connected to the turntable (65), the humidity sensor (9), the temperature sensor (10) and the travel switch (67) are connected with the input end of the PLC, and the PLC controls a water pump (38), a speed reducing motor (64), an air sterilizer (74), an air heater (71) and each first electromagnetic valve and each second electromagnetic valve; the spray nozzle (33) comprises a sleeve (331) fixedly connected to the spray branch pipe (32), the lower end of the sleeve (331) is fixedly connected with a ring cover (337), a shaft lever (332) is arranged in the ring cover (337), the shaft lever (332) is fixedly connected with a piston (338) arranged in the sleeve (331), a plurality of first drain holes (335) are formed in the piston (338), a plurality of second drain holes (334) are formed in the lower end of the sleeve (331), the piston (338) descends to the first drain holes (335) and are communicated with the second drain holes (334), a spring (333) is sleeved outside the shaft lever (332), and two ends of the spring (333) respectively prop against the piston (338) and the ring cover (337);
the culture dish (4) comprises a peripheral frame (42), and the bottom of the frame (42) is fixedly connected with a filter screen (41);
the multi-layer mounting frames (82) are sequentially arranged on two sides of the support (81) from top to bottom at intervals, a plurality of culture trays (4) are arranged in each layer of mounting frames (82), the two groups of spraying portions (3) are respectively corresponding to the culture trays (4) on two sides of the support (81), and two water splitters (34) in the two groups of spraying portions (3) are communicated through a second hose.
2. The metarhizium anisopliae culture device according to claim 1, wherein: a housing (336) is fixedly connected to the lower end of the sleeve, and each second drain hole (334) is arranged in the housing (336).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010358284.7A CN111378575B (en) | 2020-04-29 | 2020-04-29 | Metarrhizium anisopliae culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010358284.7A CN111378575B (en) | 2020-04-29 | 2020-04-29 | Metarrhizium anisopliae culture device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111378575A CN111378575A (en) | 2020-07-07 |
| CN111378575B true CN111378575B (en) | 2023-09-19 |
Family
ID=71222932
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010358284.7A Active CN111378575B (en) | 2020-04-29 | 2020-04-29 | Metarrhizium anisopliae culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111378575B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1163605A (en) * | 1997-08-11 | 1999-03-05 | Otsuka Pharmaceut Factory Inc | Ventilation method and ventilator in air shower room |
| JP2005040061A (en) * | 2003-07-23 | 2005-02-17 | Satoshi Takeuchi | Fermentation tank (jar fermenter) |
| CN1800339A (en) * | 2005-09-29 | 2006-07-12 | 江苏大学 | Integral device for inoculating and culturing for edible mushroom liquid strain |
| CN108004138A (en) * | 2017-12-27 | 2018-05-08 | 济南航晨生物科技有限公司 | A kind of agricultural probiotic solid fermentation integration apparatus |
| CN207498351U (en) * | 2017-09-20 | 2018-06-15 | 济南百斯杰生物工程有限公司 | A kind of fermentation tank |
| CN209260087U (en) * | 2018-10-23 | 2019-08-16 | 天津忆春生物科技有限公司 | A kind of fermentor air-breather |
| CN212293583U (en) * | 2020-04-29 | 2021-01-05 | 湖北中向生物工程有限公司 | Metarrhizium anisopliae solid state fermentation culture kettle |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104630020B (en) * | 2013-11-07 | 2016-08-24 | 中国科学院天津工业生物技术研究所 | A kind of solid-state biological reaction apparatus and the method preparing filamentous bacteria spore with this device |
-
2020
- 2020-04-29 CN CN202010358284.7A patent/CN111378575B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1163605A (en) * | 1997-08-11 | 1999-03-05 | Otsuka Pharmaceut Factory Inc | Ventilation method and ventilator in air shower room |
| JP2005040061A (en) * | 2003-07-23 | 2005-02-17 | Satoshi Takeuchi | Fermentation tank (jar fermenter) |
| CN1800339A (en) * | 2005-09-29 | 2006-07-12 | 江苏大学 | Integral device for inoculating and culturing for edible mushroom liquid strain |
| CN207498351U (en) * | 2017-09-20 | 2018-06-15 | 济南百斯杰生物工程有限公司 | A kind of fermentation tank |
| CN108004138A (en) * | 2017-12-27 | 2018-05-08 | 济南航晨生物科技有限公司 | A kind of agricultural probiotic solid fermentation integration apparatus |
| CN209260087U (en) * | 2018-10-23 | 2019-08-16 | 天津忆春生物科技有限公司 | A kind of fermentor air-breather |
| CN212293583U (en) * | 2020-04-29 | 2021-01-05 | 湖北中向生物工程有限公司 | Metarrhizium anisopliae solid state fermentation culture kettle |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111378575A (en) | 2020-07-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US7183074B2 (en) | Gas dual-dynamic solid state fermentation technique and apparatus | |
| CN106358752A (en) | Small-scale edible fungus planting device and planting method | |
| CN212293583U (en) | Metarrhizium anisopliae solid state fermentation culture kettle | |
| CN111378575B (en) | Metarrhizium anisopliae culture device | |
| CN111909827B (en) | Edible fungus liquid fermentation strain culture equipment | |
| CN108142210A (en) | The sterile processing systems of edible fungi raw materials | |
| CN113796265A (en) | Production base intelligent management control system for edible mushroom cultivation | |
| KR100608494B1 (en) | Apparatus for manufacturing brownrice | |
| CN109328883A (en) | A kind of culture apparatus of edible fungus species and its breeding method of edible fungus species | |
| CN214937477U (en) | Beauveria bassiana solid fermentation case | |
| CN109097262B (en) | Probiotic inoculation breeding device | |
| CN104726341B (en) | A kind of cultural method of black stiff bacterium | |
| CN218932137U (en) | Solid-state fermentation workshop for paecilomyces lilacinus agent | |
| CN116355975B (en) | Modified lentinan, preparation method and production line | |
| CN114902904B (en) | Environment disinfection equipment for succulent plant breeding | |
| CN205305511U (en) | Domestic fungus canopy intelligence control system | |
| CN203065480U (en) | Thin-layer self-control static solid fermentation device | |
| CN221510484U (en) | Novel strain cultivation device | |
| CN208883894U (en) | Novel intelligent plant immune JU compound bacteria process units | |
| CN218561435U (en) | Probiotics production is with aseptic culture apparatus | |
| CN221863900U (en) | Morchella strain cultivation frame | |
| CN212152300U (en) | Beauveria bassiana fermenting device | |
| CN221626256U (en) | Paecilomyces lilacinus agent fermentation operation room | |
| CN220545571U (en) | Morchella strain constant temperature incubator | |
| KR102360531B1 (en) | improved rotary type automatic seed culture device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20230823 Address after: 443300 Group 5, Honghuatao Village, Honghuatao Town, Yidu City, Yichang City, Hubei Province Applicant after: Hubei Zhongxiang Bioengineering Co.,Ltd. Applicant after: Zhongxiang (Hubei) Environmental Protection Technology Co.,Ltd. Address before: 443302 group 5, Honghuatao village, Honghuatao Town, Yidu City, Yichang City, Hubei Province Applicant before: Hubei Zhongxiang Bioengineering Co.,Ltd. |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |