CN218932137U - Solid-state fermentation workshop for paecilomyces lilacinus agent - Google Patents

Abstract

The utility model relates to the technical field of microorganism fermentation equipment, in particular to a paecilomyces lilacinus microbial inoculum solid-state fermentation workshop. The specific technical scheme is as follows: the utility model provides a light violet paecilomyces agent solid state fermentation workshop, includes airtight room, run through on the airtight room and be provided with at least one air intake, be provided with heating heat preservation device on the airtight room inner wall, airtight room's top is provided with temperature and humidity sensor, airtight room is provided with ozone sterilization equipment and atomizing humidification equipment outward respectively to communicate with airtight room inside through the pipeline, airtight room's top is provided with the air outlet, the air outlet has the settling chamber through the air-out pipe intercommunication, temperature and humidity sensor respectively with heating heat preservation device and atomizing humidification equipment electric connection. The utility model solves the problems of easy infection, high mixed bacteria rate and difficult control of the product quality among batches in the fermentation process of paecilomyces lilacinus in the prior art.

Description

Solid-state fermentation workshop for paecilomyces lilacinus agent

Technical Field

The utility model relates to the technical field of microorganism fermentation equipment, in particular to a paecilomyces lilacinus microbial inoculum solid-state fermentation workshop.

Background

Paecilomyces lilacinus (Paecilomyces lilacinus) belongs to Deuteromoyeotomia (HypHome), ceurosporine (HypHome), courosporine (Monilaeeae) and Paecilomyces (Paecilomyces), is a high-efficiency broad-spectrum biocontrol fungus, and has good control effect on various nematodes such as root-knot nematodes, cyst nematodes, golden nematodes, heterodera and the like. In addition, paecilomyces lilacinus also has the advantages of being adaptable to different climatic conditions, having higher parasitic rate to eggs of the nematodes, effectively controlling the quantity of soil nematodes, not polluting the environment, having abundant sources of production raw materials, being harmless to people and livestock, being difficult to generate drug resistance of target organisms and the like, and is a preferred medicament for producing high-standard organic agricultural products.

Fermentation control is one of the main influencing factors of the industrialization of the biological antibacterial preparation by using Paecilomyces lilacinus at present. At present, the paecilomyces lilacinus fermentation in China is mainly traditional open fermentation, and the fermentation belongs to open fermentation, and the fermentation process is accompanied with the risk of bacteria contamination, so that the product has the problems of high mixed bacteria rate, difficult control of product quality among fermentation batches and the like.

Therefore, providing a fermentation device for paecilomyces lilacinus with high efficiency, stability and easy large-scale application is a problem to be solved by the technicians in the field.

Disclosure of Invention

Aiming at the defects of the prior art, the utility model provides a paecilomyces lilacinus microbial inoculum solid-state fermentation workshop, which solves the problems of easy infection, high mixed bacteria rate and difficult control of the product quality among batches in the fermentation process of paecilomyces lilacinus in the prior art.

In order to achieve the above purpose, the utility model is realized by the following technical scheme:

the utility model discloses a paecilomyces lilacinus microbial inoculum solid-state fermentation workshop, which comprises a closed room, wherein at least one air inlet is formed in the closed room in a penetrating manner, a heating and heat-preserving device is arranged on the inner wall of the closed room, a temperature and humidity sensor is arranged at the top of the closed room, ozone sterilization equipment and atomization and humidification equipment are respectively arranged outside the closed room and are communicated with the interior of the closed room through pipelines, an air outlet is formed in the top of the closed room, a sedimentation chamber is communicated with the air outlet through an air outlet pipe, and the temperature and humidity sensor is respectively and electrically connected with the heating and heat-preserving device and the atomization and humidification equipment.

Preferably, the air inlet is provided with a filtering mechanism, the filtering mechanism comprises a filter cylinder which is fixed in the air inlet in a penetrating manner and is provided with two ends open, and a high-efficiency filter, a fan, a shutter, filter cotton and a steel wire mesh are sequentially arranged in the filter cylinder and from the inside to the outside of the closed chamber.

Preferably, the air outlet is provided with the filtering mechanism, the high-efficiency filter is arranged at a position close to the air outlet pipe, and the steel wire mesh is positioned in the airtight room.

Preferably, the air inlets are arranged in a plurality, and are arranged near the bottom of the airtight room.

Preferably, a drain tank is arranged at the bottom of the closed room, the drain tank is obliquely arranged, and a water accumulation tank is communicated with the lower part of the drain tank.

Preferably, the side wall and the top wall of the closed room are internally provided with heat insulation materials.

Preferably, the filter cotton is wrapped in the multi-layer gauze, the shape of the filter cotton is matched with the inner wall of the filter cartridge, the periphery of the filter cotton is fixed through a clamping plate with the cross section of a fang shape, a limit groove is formed in the inner wall of the filter cartridge, the limit groove faces to one end opening outside the closed room, and the clamping plate is matched with the limit groove.

Preferably, the periphery of the steel wire mesh is fixed through a clamping piece with a section of fang, one side of the clamping plate, facing the clamping piece, is provided with a positioning column, and the positioning column penetrates through the clamping piece and is fixed through a nut.

Preferably, a plurality of dust fall nets are vertically arranged in the settling chamber and are parallel to each other, a partition plate is transversely arranged in the settling chamber, the bottom of the dust fall net is fixed on the partition plate, a plurality of ash removal holes are formed in the partition plate, the ash removal holes are located in the areas between the dust fall nets and the side walls of the settling chamber, and a baffle plate for opening and closing the ash removal holes is slidably arranged at the bottom of the partition plate.

Preferably, the inner bottom of the settling chamber is provided with an inclined block inclined towards one side, an ash outlet is formed in the lowest position of the inclined block on the side wall of the settling chamber, an air outlet hole is formed in the upper portion of the partition plate on the side wall of the settling chamber, ash removal plates are arranged between the dust removal nets and the side wall of the settling chamber, brushes are respectively arranged on two sides of the ash removal plates and are respectively in contact with the dust removal nets and the side wall of the settling chamber, a connecting rod is arranged at the top of the ash removal plate, and the connecting rod extends out of the top of the settling chamber and is fixed through a fixing rod, and interference fit between the connecting rod and the settling chamber is achieved.

The utility model has the following beneficial effects:

1. according to the utility model, through the arranged filtering mechanism, the ventilation requirement of the strain in the fermentation process is ensured, and meanwhile, the air outlet can timely discharge toxic substances generated in the fermentation process, so that the spore growth is promoted. Meanwhile, microorganisms such as spores discharged from a fermentation workshop are subjected to sedimentation treatment in a sedimentation chamber through an air outlet, so that the influence of the spores on the environment and the cross contamination in the production process are avoided.

2. According to the utility model, through the feedback adjustment of the temperature and the humidity in the closed room, the automatic control of the temperature and the humidity in the fermentation workshop is realized, the workload of the fermentation process is reduced, and meanwhile, the quality of products among different batches is ensured by more accurate control.

3. The utility model can effectively sterilize the mixed bacteria in the workshop through the ozone sterilization equipment, and the penetrability of the ozone is more thorough than that of the traditional ultraviolet sterilization, and meanwhile, the filtering mechanism can avoid the mixed bacteria pollution in the culture process, and can effectively reduce the mixed bacteria rate of the product. Meanwhile, the humidifying equipment is arranged outside the closed room, so that the water is conveniently added for humidification in the fermentation process, and sterile water is adopted for humidification, so that the risk of bacteria contamination caused by humidification is reduced.

4. The bottom of the bent frame in the fermentation workshop is provided with the roller with lock, materials can be directly placed in the shallow tray before fermentation, and then the bent frame is pushed into the sterilization room for sterilization, so that the sterilization workload is reduced.

Drawings

FIG. 1 is a schematic diagram of the structure of the present utility model;

FIG. 2 is an enlarged view of part of A in FIG. 1;

FIG. 3 is an enlarged view of part of B in FIG. 1;

FIG. 4 is a cross-sectional view of another direction of the separator;

in the figure: the device comprises a closed room 1, a heating and heat preserving device 2, a temperature and humidity sensor 3, an ozone sterilization device 4, an atomization and humidification device 5, a sedimentation chamber 6, a filter cartridge 7, a high-efficiency filter 8, a fan 9, a shutter 10, filter cotton 11, a steel wire mesh 12, an air outlet pipe 13, a drainage tank 14, gauze 15, a clamping plate 16, a limiting groove 17, a clamping piece 18, a positioning column 19, a nut 20, a dust fall net 21, a partition plate 22, an ash removal hole 23, a baffle 24, an inclined block 25, an ash outlet 26, an air outlet 27, an ash removal plate 28, a hairbrush 29, a connecting rod 30, a fixing rod 31 and a chute 32.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

The technical means used in the examples are conventional means well known to those skilled in the art unless otherwise indicated.

Referring to fig. 1-4, the utility model discloses a paecilomyces lilacinus microbial inoculum solid-state fermentation workshop, which comprises a closed room 1, wherein the closed room is a closed space, which can be a closed room, and heat insulation materials, such as heat insulation foam boards, are arranged in the side walls and the top walls of the closed room; the heat insulation plate can also be a closed space formed by combining double-layer color steel plates, and meanwhile, heat insulation materials such as foam plates are arranged between the color steel plates. At least one air inlet is arranged on the airtight room 1 in a penetrating way, and the specific quantity is set according to actual needs. The inner wall of the airtight room 1 is provided with a heating and heat-preserving device 2 which is the existing equipment and can be electrically heated or steam heated. The top of airtight room 1 is provided with temperature and humidity sensor 3, is provided with ozone sterilization equipment 4 and atomizing humidification equipment 5 respectively outside airtight room 1 to through pipeline and airtight room 1 inside intercommunication, temperature and humidity sensor 3 respectively with heating heat preservation device 2 and atomizing humidification equipment 5 electric connection, but temperature and humidity in the airtight room of control, and combine heating heat preservation device and atomizing humidification device, temperature and humidity in the airtight room are adjusted. An air outlet is arranged at the top of the airtight room 1, and is communicated with a settling chamber 6 through an air outlet pipe 13, so that dust and the like discharged from the airtight room can be settled.

Further, the air inlet is provided with a filtering mechanism which can filter dust and impurities in the outside air. Specific: the filter mechanism comprises a filter cylinder 7 which is fixed in the air inlet in a penetrating way and is provided with two ends open, the filter cylinder 7 is transversely arranged, and a high-efficiency filter 8, a fan 9, a shutter 10, filter cotton 11 and a steel wire mesh 12 are sequentially arranged in the filter cylinder 7 and from the inside of the airtight room 1 to the outside of the airtight room 1. The fan 9 blows the outside air into the airtight room, and gradually filters the air entering the airtight room through the steel wire mesh, the filter cotton, the shutter and the high-efficiency filter. The high-efficiency filter is the existing equipment and can be obtained by direct purchase.

Furthermore, the air outlet is also provided with a filtering mechanism, the high-efficiency filter 8 is arranged at a position close to the air outlet pipe 13, and the steel wire mesh 12 is positioned in the airtight room 1. The filtering mechanism on the air outlet is identical to the filtering mechanism on the air inlet in structure, and the difference is that: on the air outlet, the cartridge filter is vertical to be set up, and the wire net is located the one end in airtight room. Meanwhile, according to actual needs, one end of the filter cartridge in the airtight room is arranged to be open, namely, one end of the filter cartridge can be arranged to be funnel-shaped, so that the air output is increased, and the filter cartridge can be particularly shown in FIG. 1. The air outlet can effectively discharge toxic substances generated in the fermentation process of the strain, ash layers possibly existing, and microorganisms such as spores generated in the fermentation process for sedimentation treatment.

Further, a plurality of air inlets are arranged near the bottom of the airtight room 1, namely, two or three air inlets are preferably arranged. The side wall of the airtight room is provided with a door, and other side walls except the side can be provided with air inlets.

Further, the bottom of the closed room 1 is provided with a drain tank 14, the drain tank 14 is arranged in an inclined manner, and a water accumulation tank (not shown in the figure) is communicated with the lower part of the drain tank 14 for draining and collecting water drops possibly generated in the culturing process. The water accumulation tank can be arranged in the airtight room or outside the airtight room and is communicated with the drainage tank through a pipeline.

Further, in order to facilitate the installation of the filter cotton and the steel wire mesh, the filter cotton and the steel wire mesh are detachably arranged. The following description of the structure is based on the filter means arranged on the air inlet. Specific: the filter cotton 11 is wrapped in the multi-layer gauze 15, the shape of the filter cotton is matched with the inner wall of the filter cartridge 7, the periphery of the filter cotton 11 is fixed through the clamping plate 16 with the cross section of fang', the inner wall of the filter cartridge 7 is provided with the limit groove 17 and is open towards one end outside the airtight chamber 1, and the clamping plate 16 is matched with the limit groove 17. The method comprises the following steps: through the limiting groove, the filter cotton fixed by the clamping plate slides into the limiting groove and is abutted. The filter cotton and the gauze can filter the inlet air well. Meanwhile, the device is convenient to detach and replace.

Further, the periphery of the steel wire mesh 12 is fixed by a clamping piece 18 with a section of fang, a positioning column 19 is arranged on one side of the clamping plate 16 facing the clamping piece 18, and the positioning column 19 penetrates through the clamping piece 18 and is fixed by a nut 20. It should be noted that: the clamping piece is only one implementation mode, and can also be a fixing plate which is directly fixed with the periphery of the steel wire mesh, and the purpose of fixing the steel wire mesh can also be achieved. The side wall of the positioning column is provided with threads, and the length of the threads is smaller than or equal to that of the positioning column, so that the positioning column can be a stud. On the reference column, the two sides that lie in the clamping piece can be provided with the nut respectively to fix the wire net on the reference column, simultaneously, accessible adjusting nut is in the position on the reference column that is close to cotton one side of filtration, adjusts the distance between cotton filtration and the wire net, can adjust according to actual need can.

Further, a plurality of dust fall nets 21 are vertically arranged in the settling chamber 6, the dust fall nets 21 are parallel to each other, a partition plate 22 is transversely arranged in the settling chamber 6, the bottom of the dust fall net 21 is fixed on the partition plate 22, a plurality of dust removal holes 23 are formed in the partition plate 22, the dust removal holes 23 are located in the areas between the dust fall nets 21 and the side walls of the settling chamber 6, and therefore ash layers in each area enter the area below the partition plate through the dust removal holes. The ash cleaning holes are preferably funnel-shaped, so that ash layers are prevented from being deposited on the partition plates. The number of the ash removing holes is set according to the size of the partition plate. Of course, the dust fall net can be replaced by other materials such as a diaphragm.

Further, a baffle 24 for opening and closing the ash cleaning hole 23 is slidably arranged at the bottom of the partition 22. Specific: the bottom both sides of baffle 22 are provided with spout 32 that the cross-section is "fang" shape respectively, and in the spout was arranged in to in the both sides of baffle to with spout adaptation, simultaneously, the bottom surface of baffle top surface butt baffle to carry out the shutoff to the deashing hole on the baffle, when the deashing is needed, the baffle is taken out, thereby makes the ash layer that falls into in the deashing hole fall into the bottom of settling chamber. And one side of the baffle plate directly extends out of the settling chamber and is provided with a handle. The baffle passes through the hole of the settling chamber and is internally provided with a rubber pad, so that the baffle is in interference fit with the settling chamber, and the ash removal hole can be closed and opened only by pulling the baffle forcefully.

Further, the inner bottom of the settling chamber 6 is provided with a sloping block 25 sloping towards one side, but of course it is also possible to provide a sloping plate, so that the ash layer falling onto the sloping block is easy to clean. The lower most part of the inclined block 25 on the side wall of the settling chamber 6 is provided with an ash outlet 26, and a door is arranged on the ash outlet to prevent ash layers from directly leaking out of the ash outlet. An air outlet 27 is arranged on the side wall of the settling chamber 6 above the partition plate 22, and a filter screen can be arranged on the air outlet according to actual needs. One end of the air outlet pipe is arranged on the side wall of the settling chamber and corresponds to the net surface of the dust fall net, and can be shown by referring to fig. 1. The aperture of the dust fall net can be consistent, and can be set into different apertures according to the requirement.

Further, in order to clean dust on the dust fall net, a dust cleaning plate 28 is arranged between the dust fall nets 21 and the side walls of the settling chamber 6, brushes 29 are respectively arranged on two sides of the dust cleaning plate 28, and of course, the periphery sides of the dust cleaning plate can be provided with brushes, so that the inner walls of the settling chamber can be cleaned, the brushes 29 are respectively contacted with the dust fall nets 21 and the side walls of the settling chamber 6, a connecting rod 30 is arranged at the top of the dust cleaning plate 28, the connecting rod 30 extends out of the top of the settling chamber 6 and is fixed through a fixing rod 31, and interference fit between the connecting rod 30 and the settling chamber 6 is achieved. It should be noted that: the connecting rod stretches out and can set up the rubber pad on the inner wall in the hole at settling chamber top, makes connecting rod and settling chamber interference fit, avoids settling chamber gas leakage, only need pull the connecting rod up-and-down motion with effort to make the brush clear up the lateral wall of dust fall net. When not in use, the ash removing plate is positioned at the inner bottom of the sedimentation chamber. Meanwhile, magnetic sheets can be respectively arranged between the top surface of the ash removal plate and the inner bottom of the settling chamber, or the magnetic sheets and the metal sheets are correspondingly arranged, so that the ash removal plate is adsorbed and fixed.

Further, in the airtight room provided by the utility model, a plurality of curved frames are arranged, the bottoms of the curved frames are provided with rollers with locks, a plurality of layers of curved plates can be placed on the curved frames, the curved plates are stainless steel shallow plates, the height of the curved plates can be 10cm, and the gap of Qu Pangao degrees is 10cm, so that the strain is fermented.

In the solid-state fermentation workshop for Paecilomyces lilacinus, paecilomyces lilacinus is subjected to solid-state fermentation, the length and width of the bent frame are 2m multiplied by 1.2m multiplied by 1.8m, the sizes of the air inlet and the air outlet are 0.6m multiplied by 0.6m, and the gauze is provided with 6 layers. The length, width and height of the closed space are 5m multiplied by 2m.

In the culture process, the formula of the culture medium is wheat: rice bran=8:2, the water content of the rice bran is regulated to 55% by 2% glucose aqueous solution before culture, the inoculation amount of paecilomyces lilacinus is 3%after high-pressure steam sterilization, the culture temperature is controlled to be 28-30 ℃, the culture humidity is controlled to be 75-80%, the fermentation is finished after 8d of fermentation, and the number of spores of paecilomyces lilacinus is measured to be 7.8x10 after the fermentation inoculant is dried and crushed in a drying workshop ⁹ The mixed bacterial rate of the bacterial agent is less than 1 percent, and the bacterial agent of the paecilomyces lilacinus is produced in a single batch of 2m ³ The net weight of the dried microbial inoculum product is 1.2t.

In the description of the present utility model, it should be understood that the terms "longitudinal," "transverse," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present utility model, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present utility model.

The above embodiments are only illustrative of the preferred embodiments of the present utility model and are not intended to limit the scope of the present utility model, and various modifications and improvements made by those skilled in the art to the technical solutions of the present utility model should fall within the protection scope defined by the claims of the present utility model without departing from the design spirit of the present utility model.

Claims (10)

1. A paecilomyces lilacinus agent solid state fermentation workshop is characterized in that: including airtight room (1), run through on airtight room (1) and be provided with at least one air intake, be provided with heating heat preservation device (2) on airtight room (1) inner wall, the top between airtight room (1) is provided with temperature and humidity sensor (3), be provided with ozone sterilization equipment (4) and atomizing humidification equipment (5) outside airtight room (1) respectively to through pipeline and airtight room (1) inside intercommunication, the top between airtight room (1) is provided with the air outlet, the air outlet has settling chamber (6) through air-out pipe (13) intercommunication, temperature and humidity sensor (3) respectively with heating heat preservation device (2) and atomizing humidification equipment (5) electric connection.

2. The solid state fermentation plant for the paecilomyces lilacinus agent according to claim 1, wherein the plant comprises: the novel air inlet is characterized in that a filtering mechanism is arranged on the air inlet and comprises a filter cylinder (7) which penetrates through the air inlet and is fixed in the air inlet and is provided with two ends, and a high-efficiency filter (8), a fan (9), a shutter (10), filter cotton (11) and a steel wire mesh (12) are sequentially arranged in the filter cylinder (7) and from the inside of the airtight room (1) to the outside of the airtight room (1).

3. The solid state fermentation plant for the paecilomyces lilacinus agent according to claim 2, wherein the plant comprises: the air outlet is provided with the filtering mechanism, the high-efficiency filter (8) is arranged at a position close to the air outlet pipe (13), and the steel wire mesh (12) is positioned in the airtight room (1).

4. The solid state fermentation plant for the paecilomyces lilacinus agent according to claim 2, wherein the plant comprises: the air inlets are arranged in a plurality, and are arranged close to the bottom of the airtight room (1).

5. The solid state fermentation plant for the paecilomyces lilacinus agent according to claim 1, wherein the plant comprises: the bottom of the airtight room (1) is provided with a drainage groove (14), the drainage groove (14) is obliquely arranged, and a water accumulation tank is communicated with the lower part of the drainage groove (14).

6. The solid state fermentation plant for the paecilomyces lilacinus agent according to claim 1, wherein the plant comprises: the side wall and the top wall of the airtight room (1) are internally provided with heat insulation materials.

7. A paecilomyces lilacinus agent solid state fermentation plant according to claim 2 or 3, characterized in that: the filter cotton (11) is wrapped in the multi-layer gauze (15), the shape of the filter cotton is matched with the inner wall of the filter cartridge (7), the periphery of the filter cotton (11) is fixed through the clamping plate (16) with the cross section of fang, the inner wall of the filter cartridge (7) is provided with the limit groove (17) and is opened towards one end outside the airtight room (1), and the clamping plate (16) is matched with the limit groove (17).

8. The solid state fermentation plant for the paecilomyces lilacinus agent according to claim 7, wherein the plant comprises: the periphery of the steel wire mesh (12) is fixed through a clamping piece (18) with a section of a fang shape, a positioning column (19) is arranged on one side of the clamping piece (18) facing the clamping plate (16), and the positioning column (19) penetrates through the clamping piece (18) and is fixed through a nut (20).

9. The solid state fermentation plant for the paecilomyces lilacinus agent according to claim 1, wherein the plant comprises: the sedimentation chamber (6) is internally and vertically provided with a plurality of dust fall nets (21) and are mutually parallel to each other between the dust fall nets (21), a baffle plate (22) is transversely arranged in the sedimentation chamber (6), the bottom of the dust fall net (21) is fixed on the baffle plate (22), a plurality of dust removal holes (23) are formed in the baffle plate (22), the dust removal holes (23) are positioned in the areas between the dust fall nets (21) and the side walls of the sedimentation chamber (6), and a baffle plate (24) for opening and closing the dust removal holes (23) is arranged at the bottom of the baffle plate (22) in a sliding mode.

10. The solid state fermentation plant for the paecilomyces lilacinus agent according to claim 9, wherein the plant comprises: the utility model discloses a sedimentation chamber, including sedimentation chamber (6), connecting rod (30) are provided with dust removal plate (28) between dust fall net (21) and sedimentation chamber (6), both sides of dust removal plate (28) are provided with brush (29) respectively, brush (29) respectively with dust fall net (21) and sedimentation chamber (6) lateral wall contact, the top of dust removal plate (28) is provided with connecting rod (30), connecting rod (30) are stretched out the top of sedimentation chamber (6) and are fixed through dead lever (31), interference fit between connecting rod (30) and sedimentation chamber (6).

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