CN215500580U - Grape downy mildew field inoculation device - Google Patents
Grape downy mildew field inoculation device Download PDFInfo
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- CN215500580U CN215500580U CN202121378172.4U CN202121378172U CN215500580U CN 215500580 U CN215500580 U CN 215500580U CN 202121378172 U CN202121378172 U CN 202121378172U CN 215500580 U CN215500580 U CN 215500580U
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- 238000011081 inoculation Methods 0.000 title claims abstract description 71
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- 241000233679 Peronosporaceae Species 0.000 title claims abstract description 19
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- 230000005540 biological transmission Effects 0.000 claims abstract description 7
- 241001281803 Plasmopara viticola Species 0.000 claims description 25
- 238000004659 sterilization and disinfection Methods 0.000 claims description 20
- 229920000742 Cotton Polymers 0.000 claims description 8
- 238000004321 preservation Methods 0.000 claims description 4
- 241000219095 Vitis Species 0.000 abstract description 28
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/12—Technologies relating to agriculture, livestock or agroalimentary industries using renewable energies, e.g. solar water pumping
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a grape downy mildew field inoculation device which comprises a support, wherein a driving assembly is arranged in the middle of the support, an inoculation box and a filter box are arranged above the support, the filter box is arranged on two sides of the inoculation box, light screens are arranged above the inoculation box and the filter box, an atomizer is arranged between the inoculation box and the light screens, the atomizer is communicated with the inoculation box, and the top of the light screens is connected with a solar assembly; be provided with the pay-off case between inoculation hood and the support, be provided with the culture part in the pay-off case, drive assembly sets up with the transmission of culture part, pay-off case and inoculation hood intercommunication. The method can accurately control the growth conditions for cultivating the grape downy mildew, utilizes solar energy to provide energy, achieves a stable cultivation environment, and is suitable for large-scale cultivation.
Description
Technical Field
The utility model belongs to the technical field of biological instruments, and particularly relates to a field grape downy mildew inoculation device.
Background
Grape downy mildew is one of the most serious diseases in grape production, and rain and heat are important conditions for the disease. The plasmopara viticola sporangium has strong fecundity, short incubation period and frequent re-infection and is a typical epidemic disease. The development of early monitoring, early warning, prevention and control and disease-resistant variety breeding of the grape downy mildew is a fundamental measure for preventing and treating the diseases. At present, researches on the etiology, epidemic rules, prevention and treatment technologies and the like of the plasmopara viticola are more, but the researches are not verified by carrying out relevant conclusions through a field inoculation test, but the reports on the field strain propagation and culture device of the plasmopara viticola are less. The plasmopara viticola is an obligate parasitic bacterium and can only grow on a living body. The traditional inoculation and strain culture method is to inoculate germs by using a culture dish and leaves of grapes in vitro, to preserve moisture in an artificial incubator or by using an agar culture medium and filter paper absorbing water, to seal gaps of the culture dish by a sealing film, and to propagate the germs. However, the culture method using the culture dish cannot perform culture in the field, a relatively sterile environment is required according to a proper season, a proper temperature and a proper humidity, and an external environment condition is insufficient for culturing a large amount of plasmopara viticola, because the microenvironment such as temperature, humidity, illumination and the like in the field changes in real time, even in the proper season, under the operation of the traditional inoculation method, the culture period of the plasmopara viticola is long, the culture quality cannot be guaranteed, and the plasmopara viticola has a physiological differentiation phenomenon, and the infection capacity and pathogenicity of different physiological species are different. Due to the difference of the inoculation method and the influence of the field microenvironment, the real infection capacity of the strain of the plasmopara viticola is difficult to reflect on the activity of different physiological races of the plasmopara viticola, so that the accuracy of test data is reduced. Therefore, in production, a plasmopara viticola field inoculation device is urgently needed to meet the research on the aspects of the genetics, the pathogenic mechanism, the breeding and utilization of disease-resistant varieties, the prevention and the treatment of the plasmopara viticola.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a field inoculation device for plasmopara viticola, which solves the problems.
In order to achieve the purpose, the utility model provides the following scheme: the grape downy mildew field inoculation device comprises a support, wherein a driving assembly is arranged in the middle of the support, an inoculation box and a filter box are arranged above the support, the filter box is arranged on two sides of the inoculation box, light screens are arranged above the inoculation box and the filter box, an atomizer is arranged between the inoculation box and the light screens, the atomizer is communicated with the inoculation box, and the top of the light screens is connected with a solar assembly;
a feeding box is arranged between the inoculation box and the support, a culture part is arranged in the feeding box, the driving assembly is in transmission arrangement with the culture part, and the feeding box is communicated with the inoculation box.
Preferably, the filter box comprises an intake filter box and an exhaust filter box, a second air inlet is communicated between the intake filter box and the seed receiving box, a first exhaust port is communicated between the exhaust filter box and the seed receiving box, and the second air inlet and the first exhaust port are respectively arranged above the seed receiving box; a first air inlet is arranged below the outer side of the air inlet filter box, and a second air outlet is arranged below the outer side of the exhaust filter box.
Preferably, the filter box admits air with be provided with filter unit in the exhaust filter box respectively, filter unit includes first ultraviolet ray disinfection lamp and cotton wool, first ultraviolet ray disinfection lamp sets up respectively the filter box admits air with the inboard top of exhaust filter box, the cotton wool sets up in first air inlet, second air inlet, first exhaust port and the second exhaust port.
Preferably, a plurality of second ultraviolet disinfection lamps are arranged in the inoculation box, two operation windows are arranged on the outer side face of the inoculation box, and an observation window is arranged above the operation windows.
Preferably, a plurality of culture plates are vertically placed in the feeding box, and a telescopic rod is connected between the adjacent culture plates and the same side of each culture plate.
Preferably, the driving assembly comprises a servo motor, the servo motor is fixedly connected to the middle of the support, a lead screw is coupled to the upper portion of the servo motor in a shaft connection mode, a sliding block is connected to the lead screw in a threaded mode, two lifting rings are connected to the upper portion of the sliding block in a transmission mode, and the two lifting rings are fixedly connected to one side, close to the telescopic rod, of the culture plate on the top; the slider is provided with two hooks, and the hooks are matched with the hanging rings.
Preferably, the inside of the seed receiving box, the inside of the air inlet filter box and the inside of the exhaust filter box are relatively sealed, and the outer sides of the seed receiving box, the air inlet filter box and the exhaust filter box are respectively provided with a heat insulation layer.
Preferably, the solar module is electrically connected with the servo motor, the atomizer, the first ultraviolet disinfection lamp and the second ultraviolet disinfection lamp respectively.
The utility model has the following technical effects: the driving assembly is mainly used for feeding the plasmopara viticola into a relatively sterile inoculation box, and the feeding process is ensured to be free of infection of mixed bacteria; the inoculation box mainly provides a suitable growth environment for the plasmopara viticola, is not influenced by the external environment and can be used for large-scale cultivation; the atomizer uniformly sprays the plasmopara viticola solution on the surface of the inoculated object, so that time and labor are saved on one hand, and the humidity of the inoculation box is effectively controlled on the other hand; the filter box can ventilate the inoculation box and adjust the internal temperature on one hand, and can prevent external bacteria from entering and prevent the internal plasmopara viticola from diffusing to the outside during ventilation on the other hand; utilize solar energy component to provide electric energy and heat energy for rose box, drive assembly, inoculation case during cultivateing, adjust the humiture, the energy can be saved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise.
FIG. 1 is a front view of an inoculating device;
FIG. 2 is a top perspective view of the inoculating device;
FIG. 3 is a right side view of the slider;
FIG. 4 is a top view of the slider;
FIG. 5 is a right side view of the plate;
wherein, 1, a bracket; 2. a servo motor; 3. a screw rod; 4. a slider; 401. hooking; 5. an intake air filter box; 6. an inoculation chamber; 7. a first air inlet; 8. a second air inlet; 9. a visor; 10. an atomizer; 11. a solar module; 12. operating a window; 13. a feeding box; 14. culturing the plate; 15. a hoisting ring; 16. a telescopic rod; 17. an exhaust filter box; 18. a first exhaust port; 19. a second exhaust port; 20. a first ultraviolet disinfection lamp; 21. a second ultraviolet disinfection lamp.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
Referring to fig. 1-5, the utility model provides a grape downy mildew field inoculation device, which comprises a support 1, wherein a driving assembly is arranged in the middle of the support 1, a seed receiving box 6 and a filter box are arranged above the support 1, the filter box is arranged on two sides of the seed receiving box 6, a light screen 9 is arranged above the seed receiving box 6 and the filter box, an atomizer 10 is arranged between the seed receiving box 6 and the light screen 9, the atomizer 10 is communicated with the seed receiving box 6, and the top of the light screen 9 is connected with a solar assembly 11;
be provided with feed box 13 between inoculation hood 6 and the support 1, be provided with the cultivation portion in the feed box 13, drive assembly sets up with the transmission of cultivation portion, feed box 13 and inoculation hood 6 intercommunication. The driving assembly mainly feeds the plasmopara viticola into the relatively sterile inoculation box 6, and ensures that no infectious microbes are infected in the feeding process; the inoculation box 6 mainly provides a suitable growth environment for the plasmopara viticola, is not influenced by the external environment and can realize mass cultivation; the atomizer 10 uniformly sprays the downy mildew spore suspension on the surface of the inoculated object, so that on one hand, time and labor are saved, and on the other hand, the humidity of the inoculation box 6 is effectively controlled; the filter box can ventilate the inoculation box 6 and adjust the internal temperature on one hand, and can prevent external bacteria from entering and prevent internal bacteria from diffusing to the outside during ventilation on the other hand; the solar energy component 11 is utilized to provide electric energy and heat energy for the filter box, the driving component and the inoculation box 6 during the cultivation period, and the temperature and the humidity are adjusted.
According to a further optimization scheme, the filter box comprises an air inlet filter box 5 and an air outlet filter box 17, a second air inlet 8 is communicated between the air inlet filter box 5 and the seed receiving box 6, a first air outlet 18 is communicated between the air outlet filter box 17 and the seed receiving box 6, and the second air inlet 8 and the first air outlet 18 are respectively arranged above the seed receiving box 6; a first air inlet 7 is arranged at the lower part of the outer side of the air inlet filter box 5, and a second air outlet 19 is arranged at the lower part of the outer side of the exhaust filter box 17.
Further optimize the scheme, be provided with the filter unit in filter box 5 and the exhaust filter box 17 of admitting air respectively, the filter unit includes first ultraviolet disinfection lamp 20 and cotton wool, and first ultraviolet disinfection lamp 20 sets up respectively at filter box 5 and the inboard top of exhaust filter box 17 of admitting air, and the cotton wool setting is in first air inlet 7, second air inlet 8, first exhaust port 18 and second exhaust port 19. The first ultraviolet disinfection lamp 20 and the absorbent cotton can ensure clean environment in the inoculation box 6, the air inlet filter box 5 and the exhaust filter box 17, ensure that the inlet air and the exhaust air are relatively sterile, and avoid the infection of the inside of the inoculation box 6 by mixed bacteria and the diffusion of grape downy mildew to the external environment.
According to a further optimized scheme, a plurality of second ultraviolet disinfection lamps 21 are arranged in the inoculation box 6, two operation windows 12 are formed in the outer side face of the inoculation box 6, and an observation window (not marked in the figure) is arranged above the operation windows 12. The cultivation condition is observed through the operation window 12 and the observation window during the cultivation period, and the abnormal condition can be dealt with in time.
According to a further optimized scheme, a plurality of culture plates 14 are vertically placed in the feeding box 13, and an expansion rod 16 is connected between the same sides of the adjacent culture plates 14.
According to a further optimized scheme, the driving assembly comprises a servo motor 2, the servo motor 2 is fixedly connected to the middle of the support 1, a lead screw 3 is connected to the upper portion of the servo motor 2 in a shaft mode, a sliding block 4 is connected to the lead screw 3 in a threaded mode, two lifting rings 15 are connected to the upper portion of the sliding block 4 in a transmission mode, and the two lifting rings 15 are fixedly connected to one side, close to the telescopic rod 16, of the culture plate 14 on the top portion; two hooks 401 are arranged on the sliding block 4, and the hooks 401 are matched with the hanging ring 15.
Further optimize the scheme, connect seed case 6, intake filter case 5 and exhaust filter case 17 inside sealed relatively, connect seed case 6, intake filter case 5 and exhaust filter case 17 outside to be provided with the heat preservation respectively. The heat preservation can guarantee the inside temperature of inoculation hood 6, intake filtration case 5 and exhaust filtration case 17, reaches stable state, provides stable growing environment for grape downy mildew.
According to the further optimized scheme, the solar module 11 can convert light energy into electric energy and heat energy, and energy is provided for cultivating the grape downy mildew.
In a further optimized scheme, the solar module 11 is electrically connected with the servo motor 2, the atomizer 10, the first ultraviolet disinfection lamp 20 and the second ultraviolet disinfection lamp 21 respectively.
In a further optimized scheme, a temperature and humidity detector (not shown in the figure) is arranged in the seed receiving box 6, a heater (not shown in the figure) is communicated in the seed receiving box 6, and the temperature and humidity detector and the heater are electrically connected with the solar module 11.
Further optimizing the scheme, preparing the spore suspension of the downy mildew, and adopting a spraying method to ensure that the concentration is 1 multiplied by 106each.mL-1The spore suspension of (2) is inoculated on the back of the grape leaf.
In a further optimized scheme, a layer of sterile filter paper (not shown in the figure) is adhered to the two sides of the grape leaves for moisture preservation.
The working process of the embodiment is as follows: before inoculating the grapevine mildew, ultraviolet sterilization is carried out on the first filter tank 5, the inoculation box 6, the feeding box 13, the culture plate 14, the second filter tank 17 and the air inside; the inoculation object is grape leaves, the grape leaves are cleaned before inoculation to remove surface sundry bacteria, then the treated grape leaves are placed on the sterilized culture plate 14, and then the culture plate 14 is placed in the feeding box 13.
After the pay-off case 13 is put on support 1, start servo motor 2, drive slider 4 rectilinear motion that makes progress through lead screw 3, in the slider 4 in-process that rises, couple 401 corresponds and gets into rings 15, then slider 4 drives the culture plate 14 at top and rises, telescopic link 16 is stretched simultaneously, until telescopic link 16 is stretched to the longest, then second culture plate 14 draws the rising through first telescopic link 16 by tensile, next telescopic link 16 is stretched in proper order, until last culture plate 14 from the pay-off case 13 by the ascending income seed receiving case 6, relative sealing between seed receiving case 6 and the pay-off case 13 this moment.
The method comprises the following steps of (1) directionally spraying a prepared grape downy mildew solution with a certain proportion into an inoculation box 6 through an atomizer 10, and uniformly dropping fine water drops carrying the grape downy mildew onto the surfaces of grape leaves; when the moisture detector detects that the moisture of the inoculation hood 6 reaches a predetermined value, the atomizer 10 stops spraying the solution, and the grape leaves have reached the expected distribution density of the grape downy mildew, and the moisture does not affect the grape leaves to deteriorate.
When the spore of the germ starts to germinate at the temperature of more than 11 ℃ of the environment, the spore is most suitable at the temperature of 22-25 ℃, the environment humidity is 95% -100%, and the germ is infected after the spore stays on the grape leaves with water drops for more than 4-6 h. The atomizer 10 is communicated with a 5L purified water barrel, and the purified water barrel is sprayed for 1 time every 1h by siphoning or pumping water, so that the humidity of the inner box is ensured to be more than 85 percent, the germs can be infected in 6-12h, the primary infection symptom (oil stain period) is displayed after 18-24h, and the mildew layer appears on the back of the blade after about 48 h. Keeping moisture above 85%, forming disease spots on all grape leaves in the inoculation box after 3-5 days from inoculation, and covering more than 20-50% of the back of the leaves with mildew layer (different grape varieties).
During the period, the humidity and the temperature in the inoculation box 6 are adjusted in time according to the detection condition of the temperature and humidity detector, and the cultivation condition can be monitored and operated through the observation window and the operation window 12; the air entering the inoculation box 6 is firstly disinfected and dehumidified by the first filter box 5, so that the influence of infectious microbes on the cultivation of the grape downy mildew is avoided; the air discharged from the inoculation box 6 enters the second filter box 17, passes through the absorbent cotton and the first ultraviolet disinfection lamp 20, and kills germs from the inoculation box 6 and prevents the germs from spreading to grape leaves in the field.
In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description of the present invention, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (8)
1. Grape downy mildew field inoculation device which characterized in that: the solar energy collecting device comprises a support (1), wherein a driving assembly is arranged in the middle of the support (1), a seed receiving box (6) and a filter box are arranged above the support (1), the filter box is arranged on two sides of the seed receiving box (6), a light screen (9) is arranged above the seed receiving box (6) and the filter box, an atomizer (10) is arranged between the seed receiving box (6) and the light screen (9), the atomizer (10) is communicated with the seed receiving box (6), and a solar energy assembly (11) is connected to the top of the light screen (9);
a feeding box (13) is arranged between the inoculation box (6) and the support (1), a culture part is arranged in the feeding box (13), the driving component is in transmission arrangement with the culture part, and the feeding box (13) is communicated with the inoculation box (6).
2. A field inoculation device for plasmopara viticola according to claim 1, wherein: the filter box comprises an air inlet filter box (5) and an air outlet filter box (17), a second air inlet (8) is communicated between the air inlet filter box (5) and the seed receiving box (6), a first air outlet (18) is communicated between the air outlet filter box (17) and the seed receiving box (6), and the second air inlet (8) and the first air outlet (18) are respectively arranged above the seed receiving box (6); a first air inlet (7) is arranged below the outer side of the air inlet filter box (5), and a second air outlet (19) is arranged below the outer side of the exhaust filter box (17).
3. A field inoculation device for plasmopara viticola according to claim 2, wherein: intake filter case (5) with be provided with the filter unit in exhaust filter case (17) respectively, the filter unit includes first ultraviolet ray disinfection lamp (20) and cotton wool, first ultraviolet ray disinfection lamp (20) set up respectively intake filter case (5) with exhaust filter case (17) inboard top, the cotton wool sets up in first air inlet (7), second air inlet (8), first exhaust port (18) and second exhaust port (19).
4. A field inoculation device for Plasmopara viticola according to claim 3, wherein: a plurality of second ultraviolet disinfection lamps (21) are arranged in the inoculation box (6), two operation windows (12) are arranged on the outer side face of the inoculation box (6), and an observation window is arranged above the operation windows (12).
5. A field inoculation device for Plasmopara viticola according to claim 4, wherein: a plurality of culture plates (14) are vertically placed in the feeding box (13), and telescopic rods (16) are connected between the adjacent culture plates (14) at the same side.
6. A field inoculation device for Plasmopara viticola according to claim 5, wherein: the driving assembly comprises a servo motor (2), the servo motor (2) is fixedly connected to the middle of the support (1), a lead screw (3) is connected to the upper portion of the servo motor (2) in a shaft mode, a sliding block (4) is connected to the lead screw (3) in a threaded mode, two lifting rings (15) are connected to the upper portion of the sliding block (4) in a transmission mode, and the two lifting rings (15) are fixedly connected to one side, close to the telescopic rod (16), of the culture plate (14) at the top; two hooks (401) are arranged on the sliding block (4), and the hooks (401) are matched with the hanging rings (15).
7. A field inoculation device for plasmopara viticola according to claim 2, wherein: the inside of the inoculation hood (6), the inside of the air inlet filter box (5) and the inside of the exhaust filter box (17) are relatively sealed, and the outer sides of the inoculation hood (6), the air inlet filter box (5) and the exhaust filter box (17) are respectively provided with a heat preservation layer.
8. A field inoculation device for Plasmopara viticola according to claim 6, wherein: the solar component (11) is respectively electrically connected with the servo motor (2), the atomizer (10), the first ultraviolet disinfection lamp (20) and the second ultraviolet disinfection lamp (21).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121378172.4U CN215500580U (en) | 2021-06-21 | 2021-06-21 | Grape downy mildew field inoculation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121378172.4U CN215500580U (en) | 2021-06-21 | 2021-06-21 | Grape downy mildew field inoculation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215500580U true CN215500580U (en) | 2022-01-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121378172.4U Expired - Fee Related CN215500580U (en) | 2021-06-21 | 2021-06-21 | Grape downy mildew field inoculation device |
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| Country | Link |
|---|---|
| CN (1) | CN215500580U (en) |
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2021
- 2021-06-21 CN CN202121378172.4U patent/CN215500580U/en not_active Expired - Fee Related
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Granted publication date: 20220114 |