CN111378575A - Metarrhizium anisopliae culture apparatus - Google Patents
Metarrhizium anisopliae culture apparatus Download PDFInfo
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- CN111378575A CN111378575A CN202010358284.7A CN202010358284A CN111378575A CN 111378575 A CN111378575 A CN 111378575A CN 202010358284 A CN202010358284 A CN 202010358284A CN 111378575 A CN111378575 A CN 111378575A
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- C12M23/00—Constructional details, e.g. recesses, hinges
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Abstract
The Metarrhizium anisopliae culture device comprises an autoclave, a ferry vehicle and a plurality of sections of steam-curing vehicles, and also comprises a culture tray placing frame, a spraying part and a ventilating part; cultivate a set rack: the device comprises brackets fixedly connected to each steam-curing car, wherein a plurality of layers of mounting frames are sequentially arranged on the brackets at intervals from top to bottom, and a plurality of culture trays are arranged in each layer of mounting frame; and (3) a spraying part: the device comprises spraying branch pipes arranged above each culture disc and a plurality of spraying main pipes which are respectively and fixedly connected in an autoclave at one side of each layer of mounting rack; and (3) a ventilation part: the device comprises an axial flow fan, an air inlet pipe and an exhaust pipe which are respectively communicated with two ends of a still kettle, wherein the fan is arranged on the air inlet pipe, an air suction port of the fan is communicated with an air filter and an air sterilizer through an air suction pipe, and an air heater is arranged on the air inlet pipe. The invention is used for solving the problems that the prior metarhizium anisopliae culture is easy to mix with mixed bacteria, the production efficiency is low and the labor intensity of workers is high.
Description
Technical Field
The invention relates to a metarhizium anisopliae culture device.
Background
The green muscardine fungus is broad-spectrum entomopathogenic fungus, the number of green muscardine fungus host insects reaches more than 200 according to statistics, and the green muscardine fungus host insects can parasitize tortoise shells, weevils, golden moths, lepidoptera pest larvae, hemiptera stinkbug and the like. Can induce insects to generate green muscardine and can form repeated infection in the population. In application, the metarhizium anisopliae is mainly used for preventing and controlling pests, and the metarhizium anisopliae is developed into a fungal pesticide second to beauveria bassiana only in terms of prevention and control scale. The Metarrhizium anisopliae is harmless to human and livestock, safe to natural enemy insects, and free of environmental pollution. The culture of the metarhizium anisopliae is generally carried out by adopting a plastic bag method, the culture process comprises solid culture medium sterilization, cooling inoculation culture, bagging, ventilation and air drying culture and the like, the culture process needs to repeatedly contact the culture medium with the outside air, not only is the culture medium easily polluted by mixed bacteria, but also the culture room, the plastic bag, the culture tray and the like need manual disinfection, the production efficiency is influenced, and the labor intensity of workers is higher.
Disclosure of Invention
The invention aims to provide a metarhizium anisopliae culture device, which is used for solving the problems that the existing metarhizium anisopliae culture is easy to mix with mixed bacteria, the production efficiency is low and the labor intensity of workers is high.
In order to solve the above problems, the technical solution to be solved by the present invention is:
the Metarrhizium anisopliae culture device comprises an autoclave, a ferry vehicle and a plurality of sections of steam-curing vehicles, and also comprises a culture tray placing frame, a spraying part and a ventilating part;
cultivate a set rack: the device comprises brackets fixedly connected to each steam-curing car, wherein a plurality of layers of mounting frames are sequentially arranged on the brackets at intervals from top to bottom, and a plurality of culture trays are arranged in each layer of mounting frame;
and (3) a spraying part: the device comprises spray branch pipes arranged above each culture tray, a plurality of spray header pipes fixedly connected in an autoclave at one side of each layer of mounting rack, a suspension storage tank and a water distributor arranged outside the autoclave, wherein each spray header pipe is communicated with the water distributor through a liquid inlet pipe;
and (3) a ventilation part: the device comprises an axial flow fan, an air inlet pipe and an exhaust pipe which are respectively communicated with two ends of a still kettle, wherein the fan is arranged on the air inlet pipe, an air suction port of the fan is communicated with an air filter and an air sterilizer through an air suction pipe, and an air heater is arranged on the air inlet pipe.
The multi-layer mounting rack is arranged on two sides of the support at intervals from top to bottom in sequence, a plurality of culture trays are placed in each layer of mounting rack, the spraying parts are provided with two groups, two groups of spraying parts correspond to the culture trays on two sides of the support respectively, and two water distributors in the two groups of spraying parts are communicated through the second hose.
Still including evaporating foster car drive arrangement, evaporate foster car drive arrangement and include a plurality of axle beds of fixed connection in still cauldron, be equipped with the pivot in a plurality of axle beds, install the gear in the pivot, pivot one end is passed and is connected with the gear motor transmission of fixed setting behind the still cauldron, installs the rack with gear engaged with at the bottom surface of evaporating foster car.
Still include control system, control system includes PLC, the travel switch who sets up in pivot one side, a plurality of first solenoid valve of installing respectively on each feed liquor pipe, install the second solenoid valve on the blast pipe, install humidity transducer and the temperature sensor who evaporates on pressing the cauldron, at the epaxial carousel of rotation, fixedly connected with is used for triggering stroke switch's the piece that hits on the carousel, humidity transducer, the PLC input is connected to temperature sensor and travel switch, PLC control water pump, gear motor, air disinfector, air heater, each first solenoid valve and second solenoid valve.
The spray nozzle comprises a sleeve fixedly connected to the spray branch pipe, the lower end of the sleeve is fixedly connected with a ring cover, a shaft rod is arranged in the ring cover, the shaft rod is fixedly connected with a piston arranged in the sleeve, a plurality of first drainage holes are formed in the piston, a plurality of second drainage holes are formed in the lower end of the sleeve, the piston is communicated with the first drainage holes and the second drainage holes in a descending mode, a spring is sleeved outside the shaft rod, and two ends of the spring are respectively abutted to the piston and the ring cover.
The lower end of the sleeve is fixedly connected with a housing, and each second drain hole is arranged in the housing.
The culture tray comprises a peripheral frame, and the bottom of the frame is fixedly connected with a filter screen.
The invention has the beneficial effects that: the method has the advantages that the operation in the autoclave for sterilizing, inoculating and culturing the metarhizium anisopliae culture medium is centralized, the pollution in the culture process is effectively prevented, the production process is simplified, the production efficiency is improved, meanwhile, the metarhizium anisopliae is less irradiated by light, and the spore yield of the metarhizium anisopliae is improved.
Drawings
The invention is further described below with reference to the accompanying drawings:
FIG. 1 is a schematic front sectional view of the present invention,
fig. 2 is a schematic structural view of a front view cross section of the present invention, in which a spraying part is not shown,
FIG. 3 is a schematic front sectional view showing the structure of the present invention, in which the culture dish rack is not shown,
FIG. 4 is a schematic top sectional view of the present invention, in which the culture dish rack is not shown,
FIG. 5 is a schematic cross-sectional view taken along line A-A in FIG. 1,
FIG. 6 is a schematic view of the structure of the nozzle of the present invention,
figure 7 is a schematic view of the present invention with respect to the structure of the nozzle,
fig. 8 is a schematic view of the connection relationship between the electrical components according to the present invention.
In the figure: the ferry vehicle 1, the autoclave 2, the spraying part 3, the spraying main pipe 31, the spraying branch pipe 32, the nozzle 33, the pipe sleeve 331, the shaft rod 332, the spring 333, the second water discharging hole 334, the first water discharging hole 335, the cover 336, the ring cover 337, the water separator 34, the first valve 35, the liquid inlet pipe 36, the suspension storage tank 37, the water pump 38, the first hose 39, the culture tray 4, the filter screen 41, the frame 42, the steam-curing vehicle 5, the steam-curing vehicle driving device 6, the rotating shaft 61, the gear 62, the rack 63, the speed reduction motor 64, the rotating disc 65, the collision block 66, the travel switch 67, the ventilation part 7, the air heater 71, the air inlet pipe 72, the fan 73, the air sterilizer 74, the air filter 75, the second valve 76, the exhaust pipe 77, the culture tray placing frame 8, the bracket 81, the mounting frame 82, the humidity sensor 9.
Detailed Description
As shown in fig. 1 to 8, the metarhizium anisopliae culture device comprises an autoclave 2, a ferry vehicle 1, a plurality of sections of steam-curing vehicles 5, a culture tray placing frame 8, a spraying part 3 and a ventilating part 7;
cultivate dish rack 8: the device comprises a bracket 81 fixedly connected to each steam-curing car 5, a plurality of layers of mounting brackets 82 are sequentially arranged on the bracket 81 from top to bottom at intervals, and a plurality of culture trays 4 are arranged in each layer of mounting bracket 82;
a spraying part 3: the device comprises a spray branch pipe 32 arranged above each culture disc 4, a plurality of spray header pipes 31 fixedly connected to one side of each layer of mounting rack 82 in an autoclave 2, a suspension storage tank 37 and a water separator 34 arranged outside the autoclave 2, wherein each spray header pipe 31 is communicated with the water separator 34 through a liquid inlet pipe 36, the suspension storage tank 37 is communicated with the water separator 34 through a first hose 39, a water pump 38 is arranged on the first hose 39, a first valve 35 is arranged on the liquid inlet pipe 36, a plurality of nozzles 33 are arranged on the spray branch pipes 32, and when each culture disc 4 carried by an autoclave vehicle enters the autoclave 2, the spray branch pipes 32 and the nozzles 33 cannot collide with the culture discs 4 and the culture disc placing racks 8;
the ventilation portion 7: the device comprises an axial flow fan 73, an air inlet pipe 72 and an exhaust pipe 77 which are respectively communicated with two ends of the still kettle 2, wherein a second valve 76 is arranged on the exhaust pipe 77, the fan 73 is arranged on the air inlet pipe 72, an air suction port of the fan 73 is communicated with an air filter 75 and an air sterilizer 74 through an air suction pipe, and an air heater 71 is arranged on the air inlet pipe 72; the air filter 75 filters dust in the air, and the air sterilizer 74 sterilizes bacteria in the outside air, wherein the air filter 75 is a plate-and-frame air filter 75, the air sterilizer 74 can be a "Lianta brand pipe-inserted ultraviolet C air sterilizer 74", and the bacteria purification rate is above 99.96%.
The using method of the invention comprises the following steps:
the first step is as follows: after the prepared solid culture medium is flatly paved in each culture dish 4, the culture dish 4 is sent into the still kettle 2 by the steam-curing vehicle 5, and then the kettle doors on two sides are closed.
The second step is that: steam with the temperature of 115 ℃ is sent into the autoclave 2, and the solid culture medium is sterilized by the steam for 70 minutes.
The third step: starting the fan 73 and the air sterilizer 74, sending the external cold air into the autoclave 2, and reducing the temperature of the solid culture medium to below 30 ℃ or waiting for the solid culture medium to be naturally cooled.
The fourth step: the suspension tank 37 is filled with the metarhizium anisopliae inoculation suspension, then the water pump 38 is started, and the inoculation suspension is sprayed to the solid culture medium, so that the metarhizium anisopliae inoculation is completed.
The fifth step: after the inoculation of the metarhizium anisopliae is finished, the fan 73 and the air disinfector are started for 744 minutes every 1 hour, the sterilized air is sent to the still kettle 2, and the still kettle 2 is kept ventilated for 8-10 days.
And a sixth step: and (3) starting the air heater 71, heating the air to about 35 ℃, then sending the air into the autoclave 2, and drying the air by hot air for 36 hours to finish the drying of the metarhizium anisopliae.
The seventh step: and opening kettle doors at two ends of the still kettle 2, pulling the culture disc 4 out of the still kettle 2 by the steam-curing vehicle 5, and obtaining green spores in the culture disc 4 as the green muscardine fungus crude product.
Through the steps, the whole process of culturing the metarhizium anisopliae is completed in the still kettle 2, and the air for feeding the metarhizium anisopliae is also fed after being disinfected, so that the pollution of the metarhizium anisopliae to external bacteria can be greatly reduced, meanwhile, the whole culturing process of the metarhizium anisopliae cannot be illuminated, and the spore yield of the metarhizium anisopliae is improved.
Both sides are provided with multilayer mounting bracket 82 by last interval in proper order down at support 81, have all placed a plurality of cultivations dish 4 in each layer mounting bracket 82, spray portion 3 has two sets ofly, and two sets of spray portion 3 correspond respectively support 81 both sides and respectively cultivate dish 4, pass through the second hose switch-on between two water knockout drums 34 in two sets of spray portion 3. The design of the culture tray placing rack 8 with the structure is more reasonable, and the weight balance of the two sides of the support 81 is kept.
Still including evaporating foster car drive arrangement 6, evaporate foster car 5 drive arrangement and include a plurality of axle beds of fixed connection in still kettle 2, be equipped with pivot 61 in a plurality of axle beds, install gear 62 on the pivot 61, pivot 61 one end is passed still kettle 2 back and is connected with the gear motor 64 transmission of fixed setting, evaporate foster car 5 bottom surface and install rack 63 with gear 62 looks meshing.
When the nozzle 33 sprays the inoculation suspension to the solid culture medium, the speed reduction motor 64 can rotate in a positive and negative alternating mode to drive the culture disc 4 to move back and forth left and right through the gear 62 and the gear 62, so that the inoculation suspension is uniformly sprayed to the culture medium, and the metarhizium anisopliae can be uniformly cultured.
The automatic control system comprises a PLC, a travel switch 67 arranged on one side of a rotating shaft 61, a plurality of first electromagnetic valves respectively installed on the liquid inlet pipes 36, a second electromagnetic valve installed on an exhaust pipe 77, a humidity sensor 9 and a temperature sensor 10 installed on the still kettle 2, a rotary disc 65 on the rotating shaft 61, a collision block 66 used for triggering the travel switch 67 is fixedly connected to the rotary disc 65, the humidity sensor 9, the temperature sensor 10 and the travel switch 67 are connected with the input end of the PLC, and the PLC controls the water pump 38, the speed reducing motor 64, the air sterilizer 74, the air heater 71, the first electromagnetic valves and the second electromagnetic valves. The first valve 35 and the second valve 76 may be replaced with a first solenoid valve and a second solenoid valve.
After the solid culture is disinfected and cooled, the control system is used for culturing the metarhizium anisopliae, and the implementation process comprises the following steps:
the first step is as follows: the PLC controls the speed reducing motor 64 to be started, and when the collision block 66 collides with the travel switch 67 twice, the PLC controls the speed reducing motor 64 to rotate in a reversing way, so that the culture medium reciprocates, and simultaneously controls the water pump 38 to be started and the first electromagnetic valves to be opened for 20 seconds and then closed so as to uniformly spray the inoculation suspension onto the culture medium.
The second step is that: after the inoculation of the metarhizium anisopliae is finished, the PLC controls the fan 73, the air sterilizer 74 and the second electromagnetic valve to be opened for 4 minutes every 1 hour so as to send the sterilized air into the still kettle 2, and the still kettle 2 is kept ventilated for 8-10 days.
The third step: the PLC controls the air heater 71, the fan 73 and the air sterilizer 74 to be started, air with the temperature of about 35 ℃ is then sent into the autoclave 2, and the metarhizium anisopliae is dried.
The seventh step: after the metarhizium anisopliae is dried, kettle doors at two ends of the still kettle 2 are opened, the steam-curing vehicle 5 pulls the culture disc 4 out of the still kettle 2, and the green spores in the culture disc 4 are the crude metarhizium anisopliae.
Therefore, the automatic control of the culture of the metarhizium anisopliae is realized, the labor intensity of workers is reduced, and the culture quality of the metarhizium anisopliae of each batch is kept uniform.
The nozzle 33 comprises a sleeve 331 fixedly connected to the spray branch pipe 32, the lower end of the sleeve 331 is fixedly connected to a ring cover 337, a shaft rod 332 is arranged in the ring cover 337, the shaft rod 332 is fixedly connected with a piston 338 arranged in the sleeve, a plurality of first drainage holes 335 are arranged in the piston 338, a plurality of second drainage holes 334 are arranged at the lower end of the sleeve 331, the piston 338 moves downwards to the first drainage hole 335 to be communicated with the second drainage holes 334, a spring 333 is sleeved outside the shaft rod 332, and two ends of the spring 333 are respectively abutted to the piston 338 and the ring cover 337.
After each first electromagnetic valve is opened for 20 seconds in sequence, the spraying pressure of the green muscardine fungus inoculation suspension of the spraying branch pipe 32 can be ensured, the green muscardine fungus inoculation suspension of the spraying branch pipe 32 can further push the piston 338 to move downwards to the first drain hole 335 to be communicated with the second drain hole 334, then the green muscardine fungus inoculation suspension is sprayed to the solid culture medium through the first drain hole 335 and the second drain hole 334, the shaft lever 332 is inserted into the solid culture medium, and the solid culture medium can be turned over back and forth along with the reciprocating motion of the steam-curing vehicle 5, so that the green muscardine fungus inoculation suspension is uniformly mixed into the solid culture medium; when the pressure of the spray branch pipe 32 is lost, if the water pump 38 is closed, the first electromagnetic valve is opened, the spring 333 pushes the piston 338 to move upwards, and the shaft rod 332 retracts into the sleeve 331, so that the culture tray 4 can freely enter and exit the still kettle 2, and the shaft rod 332 is prevented from colliding with the culture tray 4.
A cover 336 is fixedly connected to a lower end of the casing, and each of the second water discharge holes 334 is provided in the cover 336. The housing 336 ensures that the metarhizium inoculation suspension is sprayed downwards.
The culture tray 4 comprises a peripheral frame 42, and the bottom of the frame 42 is fixedly connected with a filter screen 41. This structure can improve the ventilation effect of the culture tray 4.
Claims (7)
1. Metarrhizium anisopliae culture apparatus evaporates foster car (5), its characterized in that including evaporating cauldron (2), ferry vehicle (1) and multisection: also comprises a culture tray placing rack (8), a spraying part (3) and a ventilation part (7);
culture dish rack (8): the device comprises a support (81) fixedly connected to each steam-curing car (5), a plurality of layers of mounting frames (82) are sequentially arranged on the support (81) from top to bottom at intervals, and a plurality of culture trays (4) are arranged in each layer of mounting frame (82);
spray part (3): the device comprises spray branch pipes (32) arranged above each culture tray (4), a plurality of spray header pipes (31) fixedly connected in an autoclave (2) on one side of each layer of mounting rack (82) respectively, and a suspension storage tank (37) and a water distributor (34) arranged outside the autoclave (2), wherein each spray header pipe (31) is communicated with the water distributor (34) through a liquid inlet pipe (36), the suspension storage tank (37) is communicated with the water distributor (34) through a first hose (39), a water pump (38) is arranged on the first hose (39), a first valve (35) is arranged on the liquid inlet pipe (36), and a plurality of nozzles (33) are arranged on the spray branch pipes (32);
ventilation section (7): the device comprises a fan (73), an air inlet pipe (72) and an exhaust pipe (77) which are respectively communicated with two ends of a still kettle (2), wherein the exhaust pipe (77) is provided with a second valve (76), the fan (73) is arranged on the air inlet pipe (72), an air suction port of the fan (73) is communicated with an air filter (75) and an air sterilizer (74) through an air suction pipe, and the air inlet pipe (72) is provided with an air heater (71).
2. The metarhizium anisopliae culture device according to claim 1, characterized in that: the culture plate is characterized in that a plurality of layers of mounting racks (82) are sequentially arranged on two sides of the support (81) from top to bottom at intervals, a plurality of culture plates (4) are placed in the mounting racks (82), the spraying part (3) is provided with two groups, the two groups of spraying parts (3) correspond to the culture plates (4) on two sides of the support (81) respectively, and two water distributors (34) in the two groups of spraying parts (3) are communicated through second hoses.
3. The metarhizium anisopliae culture device of claim 1, characterized in that: still including evaporating and maintaining car drive arrangement (6), evaporate and maintain car (5) drive arrangement and include a plurality of axle beds of fixed connection in still kettle (2), be equipped with pivot (61) in a plurality of axle beds, install gear (62) on pivot (61), pivot (61) one end is passed and is connected with fixed gear motor (64) transmission that sets up behind still kettle (2), evaporate and maintain car (5) bottom surface and install rack (63) with gear (62) engaged with.
4. The metarhizium anisopliae culture device of claim 3, wherein: still include control system, control system includes PLC, travel switch (67) of setting in pivot (61) one side, a plurality of first solenoid valves of installing respectively on each feed liquor pipe (36), install the second solenoid valve on blast pipe (77), install humidity transducer (9) and temperature sensor (10) on autoclave (2), carousel (65) on pivot (61), fixedly connected with hits piece (66) that are used for triggering travel switch (67) on carousel (65), humidity transducer (9), PLC input is connected with temperature sensor (10) and travel switch (67), PLC control water pump (38), gear motor (64), air disinfector (74), air heater (71), each first solenoid valve and second solenoid valve.
5. The metarhizium anisopliae culture device of claim 3 or 4, wherein: nozzle (33) are including sleeve pipe (331) of fixed connection on spraying branch pipe (32), sleeve pipe (331) lower extreme fixed connection ring lid (337), be equipped with axostylus axostyle (332) in ring lid (337), axostylus axostyle (332) and piston (338) fixed connection who sets up in sleeve pipe (331), be equipped with a plurality of first wash port (335) in piston (338), a plurality of second wash port (334) have been seted up at sleeve pipe (331) lower extreme, piston (338) are down to first wash port (335) and second wash port (334) intercommunication, there is spring (333) axostylus axostyle (332) overcoat, spring (333) both ends are supported respectively on piston (338) and ring lid (337).
6. The metarhizium anisopliae culture device of claim 5, wherein: a cover casing (336) is fixedly connected with the lower end of the sleeve, and each second water drain hole (334) is arranged in the cover casing (336).
7. The metarhizium anisopliae culture device of claim 5, wherein: the culture tray (4) comprises a peripheral frame (42), and the bottom of the frame (42) is fixedly connected with a filter screen (41).
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| CN202010358284.7A CN111378575B (en) | 2020-04-29 | 2020-04-29 | Metarrhizium anisopliae culture device |
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| CN202010358284.7A CN111378575B (en) | 2020-04-29 | 2020-04-29 | Metarrhizium anisopliae culture device |
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