CN212152300U

CN212152300U - Beauveria bassiana fermenting device

Info

Publication number: CN212152300U
Application number: CN202020678574.5U
Authority: CN
Inventors: 舒延岭; 曹庆梅; 陈伟; 王杰
Original assignee: Hubei Zhongxiang Bioengineering Co ltd
Current assignee: Hubei Zhongxiang Bioengineering Co ltd
Priority date: 2020-04-28
Filing date: 2020-04-28
Publication date: 2020-12-15
Anticipated expiration: 2030-04-28

Abstract

The utility model relates to a beauveria bassiana fermenting installation, including horizontal fermentation tank, the inside cross section that is equipped with of horizontal fermentation tank is square fermentation cabin, and the fermentation cabin is equipped with a plurality of cultivations frames, is equipped with the cultivation dish on the cultivation frame, is equipped with admission line and exhaust duct on the horizontal fermentation tank body, and horizontal fermentation tank still communicates with air-blower and steam generator respectively through the pipeline, horizontal fermentation tank still communicates through pipeline and seed liquid fermentation jar, seed liquid fermentation jar pass through the pipeline respectively with culture medium compounding jar and hydrogen peroxide liquid storage pot intercommunication. The utility model discloses a pulsation formula is ventilated, and the air-blower is circulating the processing again to its inside air current, improves solid matrix's air permeability, can not need machinery to turn, at the culture in-process of seed liquid, carries out the oxidation and threatens the processing, can improve the SOD enzyme of its spore and DPPH's clearance.

Description

Beauveria bassiana fermenting device

Technical Field

The utility model relates to a microbial cultivation technical field relates to beauveria bassiana fermenting installation.

Background

Beauveria bassiana is one of the insecticidal fungi widely used for biological control of pests at home and abroad, and is considered to be an entomopathogenic fungus with the most development potential. Under the solid culture condition, beauveria bassiana produces conidia, and the conidiophore cell wall thickness is generally more biostable than the blastospore.

The beauveria bassiana is produced mainly by soil methods such as indoor tray, outdoor large bed type, plastic bag culture and the like, the method takes cheap agricultural and sideline products such as drum skin and chaff as main raw materials, complex equipment is not needed, the production method is simple and easy to implement, but the production period is long, the pollution rate is high, the product quality is low and unstable, particularly, the dust concentration in a production workshop is high in the product crushing and packaging processes, and the allergic reaction of operators can be caused. The temperature and the humidity are difficult to control, the influence is large along with the change of the temperature, the physiological states of different batches of products are uneven, the quality is inconsistent, and the requirements of industrial production and preparation processing are difficult to meet.

Disclosure of Invention

In order to solve the technical problem, the utility model provides a beauveria bassiana fermenting device which can ensure the stable quality.

The scheme of the utility model is that:

the utility model provides a beauveria bassiana fermenting installation, includes horizontal fermentation tank, and horizontal fermentation tank inside is equipped with the cross section and is square fermentation cabin, and the fermentation cabin is equipped with a plurality of cultivations frames in, is equipped with the cultivation dish on the cultivation frame, is equipped with admission line and exhaust duct on the horizontal fermentation tank jar body, and horizontal fermentation tank still communicates with air-blower and steam generator respectively through the pipeline, horizontal fermentation tank still communicates with seed liquid fermentation tank through pipeline, seed liquid fermentation tank communicate with culture medium compounding jar and hydrogen peroxide liquid storage pot respectively through the pipeline.

Preferably, a humidifier is further arranged between the horizontal fermentation tank and the blower.

Preferably, the bottom in the fermentation cabin is provided with a track for the culture shelf to slide.

Preferably, a discharge port at the bottom of the seed liquid fermentation tank is provided with a filter screen, a discharge port above the filter screen is communicated with an inoculation pipeline of the horizontal fermentation tank, the inoculation pipeline comprises an inoculation main pipe and an inoculation branch pipe, and the inoculation branch pipe is positioned above the culture disc.

Preferably, the periphery of the outside of the fermentation chamber is fully distributed with heat exchange medium pipelines, and the heat exchange medium pipelines are provided with heat exchange medium feeding pipelines and heat exchange medium discharging pipelines which are communicated with a medium storage tank outside the horizontal fermentation tank.

Preferably, the culture tray comprises an X-shaped framework at the bottom and a culture surface arranged above the X-shaped framework, and the culture surface is made of non-woven fabrics.

Further preferably, a vibration motor is arranged on the outer wall of the discharge port of the seed liquid fermentation tank.

The utility model discloses beneficial effect:

1. the utility model discloses a rice is solid culture substrate, and its granule surface area is big and have the seam, and has after curing and handle and can provide the nutrition and can preserve moisture again, can satisfy the oxygen demand and the humidity demand of beauveria bassiana fermentation process, provides sufficient spore production area again, can ensure the high-efficient breed of beauveria bassiana and industrial production.

2. The utility model discloses the inside cross section that is equipped with of horizontal fermentation cylinder is square fermentation cabin, and the gas pressure that the internal portion of jar can be adjusted to last admission line and the exhaust duct of being equipped with of the horizontal fermentation cylinder jar, and the pulsation is ventilated, and the air-blower is being circulated the processing again to its inside air current, improves solid matrix's air permeability, can not need machinery to turn, simple structure, and steam generator carries out sterilization treatment and steam curing to the culture tank body and matrix and handles, guarantees that the humiture is unanimous, ensures fermentation quality. The seed liquid fermentation tank is respectively communicated with the culture medium mixing tank and the hydrogen peroxide liquid storage tank through pipelines, and oxidation stress treatment is carried out in the culture process of the seed liquid, so that the clearance rates of SOD enzyme and DPPH of spores of the seed liquid can be improved.

3. A humidifier is further arranged between the horizontal fermentation tank and the air blower, and the humidifier can be adjusted when the humidity in the tank body is insufficient.

4. The seed liquid fermentation tank bottom discharge gate is equipped with the filter screen, and the direction slope of screen cloth towards fungus liquid tank bottom also has a pressure of propelling downwards at the in-process of separation vibrations, fermentation cabin outside is covered with heat transfer medium pipeline all around and can be used for the internal temperature regulation of fermentation tank. The vibrating motor is arranged on the outer wall of the discharge port of the seed liquid fermentation tank, so that the separation speed of mycelia can be improved.

5. Cultivate the dish and include the X type skeleton of bottom and arrange the culture surface of X type skeleton top in, the culture surface material is the non-woven fabrics, can conveniently control the radiating rate of beauveria bassiana, and the thermal diffusivity is better.

Drawings

FIG. 1 is a schematic view of the apparatus of the present invention;

FIG. 2 is a schematic view of a fermentation chamber;

FIG. 3 is a schematic view of the structure of a culture dish;

wherein: the device comprises a horizontal fermentation tank 1, a fermentation cabin 2, a culture frame 3, a culture disc 4, an X-shaped framework 401, a culture surface 402, an air inlet pipeline 5, an exhaust pipeline 6, a blower 7, a steam generator 8, a seed liquid fermentation tank 9, a culture medium mixing tank 10, a hydrogen peroxide liquid storage tank 11, a humidifier 12, a track 13, a filter screen 14, an inoculation pipeline 15, an inoculation header pipe 1501, inoculation branch pipes 1502, a heat exchange medium pipeline 16 and a vibration motor 17.

Detailed Description

The invention is further described with reference to the following examples, but the scope of the invention as claimed is not limited to the scope of the examples.

Example 1

The utility model provides a beauveria bassiana fermenting installation, includes horizontal fermentation tank 1, and 1 inside cross section that is equipped with of horizontal fermentation tank is square fermentation cabin 2, is equipped with a plurality of cultivations frame 3 in the fermentation cabin 2, cultivates and is equipped with culture dish 4 on the frame 3, is equipped with admission line 5 and exhaust duct 6 on the 1 jar of body of horizontal fermentation tank, and horizontal fermentation tank 1 still communicates with air-blower 7 and steam generator 8 respectively through the pipeline, horizontal fermentation tank 1 still communicates with seed liquid fermentation tank 9 through the pipeline, seed liquid fermentation tank 9 communicates with culture medium compounding jar 10 and hydrogen peroxide liquid storage pot 11 respectively through the pipeline.

Preferably, a humidifier 12 is further arranged between the horizontal fermentation tank 1 and the blower 7.

Preferably, the bottom of the fermentation chamber 2 is provided with a track 13 for the cultivation shelf 3 to slide.

Preferably, a discharge port at the bottom of the seed liquid fermentation tank 9 is provided with a filter screen 14, a discharge port above the filter screen 14 is communicated with an inoculation pipeline 15 of the horizontal fermentation tank 1, the inoculation pipeline 15 comprises an inoculation main pipe 1501 and an inoculation branch pipe 1502, and the inoculation branch pipe 1502 is positioned above the culture disc 4.

Preferably, the periphery of the outside of the fermentation cabin 2 is fully distributed with heat exchange medium pipelines 16, and the heat exchange medium pipelines 16 are provided with heat exchange medium feeding pipelines and heat exchange medium discharging pipelines which are communicated with a medium storage tank outside the horizontal fermentation tank 1.

Preferably, the culture tray 4 comprises an X-shaped frame 401 at the bottom and a culture surface 402 arranged above the X-shaped frame 401, and the culture surface 402 is made of non-woven fabric.

Further preferably, a vibration motor 17 is arranged on the outer wall of the discharge port of the seed liquid fermentation tank 9.

The utility model discloses during the use: adding beauveria bassiana into sterile water under aseptic operation to prepare bacterial suspension, inoculating the beauveria bassiana into a seed liquid fermentation tank 9 shake flask filled with an SDY (sodium dodecyl sulfate) culture medium according to the inoculation amount of 5%, and performing shake culture for 48 hours. After 48 hours of culture, adding 4-8mmol/L hydrogen peroxide, continuing to culture for 12 hours, increasing the SOD enzyme amount of beauveria bassiana by 142 percent through hydrogen peroxide oxidation treatment, having higher clearance rate of DPPH (denaturing high Performance liquid chromatography) for hydroxyl free radicals by 46.2 percent, and then entering a culture tray 4 of a horizontal fermentation tank 1 through an inoculation pipeline for fermentation culture.

The fermentation solid culture medium is rice, the rice is placed on the culture surface 402 with a thickness of 2-3cm, and the steam generator 8 is introduced into the culture mediumSterilizing a tank body by steam and curing the rice as a culture medium, ensuring that the water content of the cured rice matrix is 40-65%, inoculating hypha liquid into a seed liquid fermentation tank 9, wherein the inoculation amount is 10-18% v/w, controlling the environmental humidity through the cooperation of an air blower 7 and a humidifier 12, ensuring that 100% is obtained in 4 days before fermentation, 80-85% is obtained in the middle 3 days, 55-65% is obtained in 3 days after fermentation, culturing for 10 days in total, introducing sterile air through an air inlet pipeline 5 in the fermentation process, keeping the pressure at 0.12-0.45Mpa, then opening an exhaust pipeline 6, releasing the pressure of a horizontal fermentation tank 1, keeping the internal pressure of the horizontal fermentation tank 1 at 0.01-0.08 Mpa, and keeping the internal pressure at 20-60 s; the foregoing pressurization and depressurization process was repeated. And (3) growing spore powder on the surface of the rice matrix, stopping fermentation at the moment, ventilating and drying for 1-2 days, and pouring the fermented product into an imported spore collecting machine in batches for separation to obtain the spore powder. The spore content of spore powder reaches 1.75-1.98 × 10¹³The water content of each spore per gram of dry weight matrix is 8-10%, and the viable spore rate is 98.5-99.6%.

The above-mentioned embodiments only represent the preferred embodiments of the present invention, and the description thereof is more specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, various changes, modifications and substitutions can be made without departing from the spirit of the invention, and these are all within the scope of the invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims

1. The utility model provides a beauveria bassiana fermenting installation, includes horizontal fermentation cylinder (1), its characterized in that: the horizontal fermentation tank (1) is internally provided with a fermentation cabin (2) with a square cross section, a plurality of culture frames (3) are arranged in the fermentation cabin (2), a culture disc (4) is arranged on each culture frame (3), an air inlet pipeline (5) and an exhaust pipeline (6) are arranged on the tank body of the horizontal fermentation tank (1), the horizontal fermentation tank (1) is communicated with an air blower (7) and a steam generator (8) through pipelines, the horizontal fermentation tank (1) is communicated with a seed liquid fermentation tank (9) through pipelines, and the seed liquid fermentation tank (9) is communicated with a culture medium mixing tank (10) and a hydrogen peroxide liquid storage tank (11) through pipelines.

2. The beauveria bassiana fermentation apparatus according to claim 1, wherein: a humidifier (12) is also arranged between the horizontal fermentation tank (1) and the blower (7).

3. The beauveria bassiana fermentation apparatus according to claim 1, wherein: the bottom in the fermentation cabin (2) is provided with a track (13) for the culture rack (3) to slide.

4. The beauveria bassiana fermentation apparatus according to claim 1, wherein: the seed liquid fermentation tank (9) bottom discharge gate is equipped with filter screen (14), and filter screen (14) top discharge gate communicates with inoculation pipeline (15) of horizontal fermentation tank (1), and inoculation pipeline (15) are including inoculation house steward (1501) and inoculation branch pipe (1502), and inoculation branch pipe (1502) are located and cultivate dish (4) top.

5. The beauveria bassiana fermentation apparatus according to claim 1, wherein: the fermentation tank (2) is externally and peripherally filled with heat exchange medium pipelines (16), and the heat exchange medium pipelines (16) are provided with heat exchange medium feeding pipelines and heat exchange medium discharging pipelines which are communicated with a medium storage tank outside the horizontal fermentation tank (1).

6. The beauveria bassiana fermentation apparatus according to claim 1, wherein: the culture tray (4) comprises an X-shaped framework (401) at the bottom and a culture surface (402) arranged above the X-shaped framework (401), and the culture surface (402) is made of non-woven fabrics.

7. The beauveria bassiana fermentation apparatus according to claim 1, wherein: and a vibration motor (17) is arranged on the outer wall of the discharge hole of the seed liquid fermentation tank (9).