CN212152312U

CN212152312U - Paecilomyces lilacinus solid-state fermentation device

Info

Publication number: CN212152312U
Application number: CN202020676761.XU
Authority: CN
Inventors: 舒延岭; 曹庆梅; 陈伟; 王杰
Original assignee: Hubei Zhongxiang Bioengineering Co ltd
Current assignee: Hubei Zhongxiang Bioengineering Co ltd
Priority date: 2020-04-28
Filing date: 2020-04-28
Publication date: 2020-12-15
Anticipated expiration: 2030-04-28

Abstract

The utility model relates to a paecilomyces lilacinus solid state fermentation device, including the fermentation cylinder, the inside fermentation cabin that is equipped with of fermentation cylinder, fermentation cabin bottom are equipped with gas distribution board, and the gas distribution board top is equipped with horizontal (mixing) shaft, is equipped with the stirring leaf on the horizontal (mixing) shaft, and horizontal (mixing) shaft passes through agitator motor drive, and horizontal (mixing) shaft top is equipped with the inoculation device, and the inoculation device passes through pipeline and seed liquid culture tank intercommunication, the fermentation cylinder pipe wall is equipped with the intermediate layer, and the intermediate layer is equipped with medium admission pipe and medium discharge pipe, and gas distribution board bottom below is. The utility model discloses cross the air and blow upward and form fluidized bed state, guarantee the ventilation requirement among the bacterial fermentation process, and the poisonous and harmful substance that produces among the fermentation process clears away, promotes the growth of spore.

Description

Paecilomyces lilacinus solid-state fermentation device

Technical Field

The utility model relates to a microbial cultivation technical field relates to paecilomyces lilacinus solid state fermentation device.

Background

Paecilomyces lilacinus belongs to endoparasitic fungi, and can not only parasitize various plant parasitic nematodes, but also parasitize many insects and take measures against some pathogenic bacteria. The main mechanism of paecilomyces lilacinus for preventing and treating nematode is egg parasitism. Paecilomyces lilacinus protease and chitinase have strong degradation effect on meloidogyne javanicus egg shells. One of the main influencing factors of the industrialization of the paecilomyces lilacinus as a biocontrol bacterium preparation is the fermentation industry. The solid-state ventilation fermentation equipment is key equipment for realizing the aerobic solid-state fermentation technology, the traditional open type is more in the aspect of China, the performance of a solid-state fermentation reactor developed by part of manufacturers is improved, the mechanical operation and the automatic control of part of parameters are easy to realize, but the reactor is small in size, is not suitable for the requirements of the modern brewing technology on large-scale and high-efficiency development and the realization of high quality of products, the equipment investment is large, the production cost is relatively high, and the process parameters are not optimized. The existing solid state fermentation reactors are developed from simple trays to mechanized fermenters, but are also all stacking fermentation. In stacking fermentation, mass transfer is a major factor limiting microbial growth and product formation due to the relative immobility of the substrate and the microbes. In addition, in the growth process of the paecilomyces lilacinus, the produced harmful substances cannot be timely eliminated to influence the yield and the germination rate of spores, and the required nutrition cannot be timely supplemented so that the solid state fermentation period is longer.

Disclosure of Invention

In order to solve the technical problem, the utility model provides a paecilomyces lilacinus solid state fermentation device blows formation fluidized bed state on through the air, guarantees the ventilation requirement among the bacterial fermentation process, and the poisonous and harmful substance who produces in the fermentation process clears away, promotes the growth of spore.

The scheme of the utility model is that:

the utility model provides a paecilomyces lilacinus solid state fermentation device, includes the fermentation cylinder, and the inside fermentation cabin that is equipped with of fermentation cylinder, fermentation cabin bottom are equipped with gas distribution board, and the gas distribution board top is equipped with horizontal (mixing) shaft, is equipped with the stirring leaf on the horizontal (mixing) shaft, and horizontal (mixing) shaft passes through agitator motor drive, and horizontal (mixing) shaft top is equipped with the inoculation device, and the inoculation device passes through pipeline and seed liquid culture tank intercommunication, the fermentation cylinder pipe wall is equipped with the intermediate layer, and the intermediate layer is equipped with medium admission pipe and medium discharge pipe, and gas distribution.

Preferably, the top of the fermentation chamber is provided with a gas inlet pipe and a gas outlet pipe.

Preferably, a gas circulating pipe is arranged above the inoculation device in the fermentation chamber and communicated with the induced draft fan.

Further preferably, the induced draft fan is communicated with the filter and the blower in sequence through a pipeline.

Preferably, a humidifier and a heater are further arranged on a pipeline between the blower and the gas distribution plate, and the humidifier and the heater are arranged in parallel.

Preferably, a culture medium material port is arranged above the gas distribution plate of the fermentation chamber, a material outlet is arranged at the lower part of the fermentation chamber, and the material outlet is communicated with the spore separator.

Preferably, the inoculation device comprises inoculation pipes which are vertically staggered with each other in the transverse and longitudinal directions, and spray heads are uniformly distributed on the inoculation pipes.

The utility model discloses beneficial effect:

1. the utility model discloses a gas distribution board blows on with paecilomyces lilacinus fermentation solid matrix through the air and forms fluidized bed state, guarantees the ventilation requirement among the bacterial fermentation process, and the poisonous and harmful substance that produces among the fermentation process retrieves through upper portion gas-circulating pipe, and the prefilter is clear away, promotes the growth of spore. The gas pressure in the fermentation tank can be adjusted to the admission line and the exhaust duct that set up, and the pulsed is ventilated, combines the air-blower to carry out convection current again to its inside air current and handles, improves solid matrix's air permeability, does not need the stirring of horizontal (mixing) shaft, the energy saving.

2. A humidifier and a heater are further arranged between the fermentation tank and the air blower, and when the humidity or the humidity in the tank body is too high, the humidifier can be adjusted through alternate use of the humidifier and the heater.

3. The inoculation device comprises inoculation pipes which are vertically and transversely staggered, the nozzles are uniformly distributed on the inoculation pipes, the inoculation uniformity in the inoculation process is ensured, and the solid culture medium and the bacterial liquid are uniformly stirred by combining with a transverse stirring shaft.

Drawings

FIG. 1 is a schematic view of the apparatus of the present invention;

FIG. 2 is a schematic structural view of an inoculating device;

wherein: the fermentation tank comprises a fermentation tank 1, a fermentation cabin 2, a gas distribution plate 3, a transverse stirring shaft 4, stirring blades 5, a stirring motor 6, an inoculation device 7, a seed liquid culture tank 8, an interlayer 9, an air blower 10, a gas inlet pipe 11, a gas outlet pipe 12, a gas circulation pipe 13, an induced draft fan 14, a filter 15, a humidifier 16, a culture medium material port 17, a material outlet port 18, a inoculation pipe 19, a spray head 20 and a heater 21.

Detailed Description

The invention is further described with reference to the following examples, but the scope of the invention as claimed is not limited to the scope of the examples.

Example 1

The utility model provides a paecilomyces lilacinus solid state fermentation device, includes fermentation cylinder 1, and 1 inside fermentation cabin 2 that is equipped with of fermentation cylinder, 2 bottoms in fermentation cabin are equipped with gas distribution board 3, and gas distribution board 3 top is equipped with horizontal (mixing) shaft 4, is equipped with stirring leaf 5 on the horizontal (mixing) shaft 4, and horizontal (mixing) shaft 4 passes through agitator motor 6 drive, and horizontal (mixing) shaft 4 top is equipped with inoculation device 7, and inoculation device 7 passes through the pipeline and cultivates jar 8 intercommunications with the seed liquid, 1 pipe wall of fermentation cylinder is equipped with intermediate layer 9, and intermediate layer 9 is equipped with medium admission pipe and medium discharge pipe, and 3 bottoms.

Preferably, a gas inlet pipe 11 and a gas outlet pipe 12 are arranged at the top of the fermentation chamber 2.

Preferably, a gas circulation pipe 13 is arranged above the inoculation device 6 in the fermentation cabin 2 and communicated with a draught fan 14.

Further preferably, the induced draft fan 14 is communicated with the filter 15 and the blower 10 in sequence through pipelines.

Preferably, a humidifier 16 and a heater 21 are further disposed on the pipeline between the blower 10 and the gas distribution plate 3, and the humidifier 16 and the heater 21 are disposed in parallel.

Preferably, a culture medium material port 17 is arranged above the gas distribution plate 3 of the fermentation cabin 2, a material outlet 18 is arranged at the lower part of the fermentation cabin 2, and the material outlet 18 is communicated with the spore separator.

Preferably, the inoculation device 7 comprises inoculation pipes 19 which are vertically staggered with each other in the transverse direction and the longitudinal direction of inoculation, and the inoculation pipes 19 are uniformly distributed with spray heads 20.

The utility model discloses during the use: the fermentation chamber 2 is filled with a culture medium through a culture medium material port 17, and the formula of the culture medium is that the corn stalks: rice bran: corn flour (0.4-0.7): (0.3-0.5): 1.3-1.7) (weight ratio), ammonium sulfate content is 0.1-0.15%, and rice washing water is added for water content adjustment, so that the water content is 35-50%. Inoculating 8 seed bacteria liquid in the seed liquid culture tank, and when the spore content of the seed bacteria liquid reaches 10⁹When the strain/g wet weight is more than 0.5-1.5%, starting the transverse stirring shaft 4 to stir the culture medium and the bacterial liquid, fermenting for 10-14 days at the temperature of 25-30 ℃ and the humidity of 75-85%. In the fermentation process, the fermentation cabin 2 is pressurized and decompressed through the gas inlet pipe 11 and the gas outlet pipe 12, the peak pressure is 150-350kPa, the peak-valley pressure is 10-30 kPa, the pressure change period is 15-20min, in addition, a fluidization state method is formed by upward blowing air through the gas distribution plate 3 to meet the ventilation requirement, harmful gas generated in the fermentation process is timely separated out, treated through the induced draft fan 14 and the filter 15, and then reflows to the air blower 10 through the air blower 10 to form a fluidized stateIn the fermentation chamber 2. The final spore yield obtained by separation is 8.23 multiplied by 10¹⁰The spore germination rate is kept at about 95% when each dry material is put into the culture medium per gram.

The above-mentioned embodiments only represent the preferred embodiments of the present invention, and the description thereof is more specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, various changes, modifications and substitutions can be made without departing from the spirit of the invention, and these are all within the scope of the invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims

1. A paecilomyces lilacinus solid state fermentation device comprises a fermentation tank (1), and is characterized in that: fermentation cylinder (1) inside is equipped with fermentation cabin (2), and fermentation cabin (2) bottom is equipped with gas distribution board (3), and gas distribution board (3) top is equipped with horizontal (mixing) shaft (4), is equipped with stirring leaf (5) on horizontal (mixing) shaft (4), and horizontal (mixing) shaft (4) are through agitator motor (6) drive, and horizontal (mixing) shaft (4) top is equipped with inoculation device (7), and inoculation device (7) are through pipeline and seed liquid culture tank (8) intercommunication, fermentation cylinder (1) pipe wall is equipped with intermediate layer (9), and intermediate layer (9) are equipped with medium admission pipe and medium discharge pipe, and gas distribution board (3) bottom below is through pipeline and air-blower (10) intercommunication.

2. The paecilomyces lilacinus solid state fermentation device of claim 1, characterized in that: the top of the fermentation cabin (2) is provided with a gas inlet pipe (11) and a gas outlet pipe (12).

3. The paecilomyces lilacinus solid state fermentation device of claim 1, characterized in that: and a gas circulation pipe (13) is arranged above the inoculation device (7) in the fermentation cabin (2) and is communicated with a draught fan (14).

4. The paecilomyces lilacinus solid state fermentation device of claim 3, characterized in that: and the induced draft fan (14) is sequentially communicated with the filter (15) and the air blower (10) through pipelines.

5. The paecilomyces lilacinus solid state fermentation device of claim 1, characterized in that: and a humidifier (16) and a heater (21) are further arranged on a pipeline between the blower (10) and the gas distribution plate (3), and the humidifier (16) and the heater (21) are arranged in parallel.

6. The paecilomyces lilacinus solid state fermentation device of claim 1, characterized in that: a culture medium material port (17) is formed in the fermentation cabin (2) and located above the gas distribution plate (3), a material discharge port (18) is formed in the lower portion of the fermentation cabin (2), and the material discharge port (18) is communicated with the spore separator.

7. The paecilomyces lilacinus solid state fermentation device of claim 1, characterized in that: the inoculation device (7) comprises inoculation pipes (19) which are vertically staggered with each other in the transverse and longitudinal directions, and spray heads (20) are uniformly distributed on the inoculation pipes (19).