CN111304082B - Biological antibody culture device and culture method - Google Patents
Biological antibody culture device and culture method Download PDFInfo
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- CN111304082B CN111304082B CN202010152651.8A CN202010152651A CN111304082B CN 111304082 B CN111304082 B CN 111304082B CN 202010152651 A CN202010152651 A CN 202010152651A CN 111304082 B CN111304082 B CN 111304082B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
Abstract
The invention discloses a biological antibody culture device and a culture method, the culture device comprises a culture box body, a bearing groove is arranged on one side of the culture box body, a storage bin is fixedly arranged on the inner side of a nutrition box body, a plurality of liquid outlet pipes are fixedly arranged at the bottom of the storage bin, a belt conveyor for conveying bearing plates is arranged on one side of the bearing groove, a support frame is arranged above the belt conveyor, two movable carrying mechanisms are movably arranged on the support frame, a support plate is horizontally arranged on the inner side of the culture box body, a plurality of support seat groups are arranged on a vertical plate along the vertical direction, and a turnover plate is hinged to the top of the support frame; this biological antibody culture apparatus has improved biological antibody's production efficiency, has realized biological antibody's mass production, adopts the technique of automatic handling among the cultivation process, need not the manpower transport, has reduced intensity of labour simultaneously, need not manual contact when adding the nutrient solution, has guaranteed safe clean cultivation environment.
Description
Technical Field
The invention relates to the technical field of biological culture equipment, in particular to a biological antibody culture device and a biological antibody culture method.
Background
Antibodies are proteins that are produced by the body as a result of stimulation by antigens and have a protective effect. It is a large Y-shaped protein secreted by plasma cells and used by the immune system to identify and neutralize foreign substances such as bacteria, viruses, etc., and is found only in body fluids such as blood of vertebrates, and the cell membrane surface of B cells thereof. Antibodies recognize a unique feature of a particular foreign object, referred to as an antigen.
Patent document (CN207330807U) discloses a quick preparation facilities of monoclonal antibody, this quick preparation facilities's culture dish directly exposes with the air in, unable temperature regulation, need artificially manually when cultivateing to the butt joint of carrying out the conveyer pipe, can't guarantee safe clean interpolation environment, this quick preparation facilities can't cultivate a large amount of biological antibodies simultaneously, this quick preparation facilities only is provided with a conveyer pipe, need add the culture dish one by one when adding the nutrient solution, and high labor strength, low in production efficiency.
Disclosure of Invention
The invention aims to provide a biological antibody culture device and a culture method.A plurality of culture dish placing grooves are formed in a bearing plate, a single bearing plate can be used for loading a plurality of culture dishes at the same time, and a plurality of bearing plates can be simultaneously accommodated in a culture box body by respectively arranging a plurality of layers of supporting seats on two vertical plates, so that the technical problems that the traditional biological antibody culture device has small accommodating amount of culture dishes, cannot culture a large amount of biological antibodies at the same time and has low production efficiency are solved;
the carrying plate is conveyed by the belt conveyor, the movable carrying mechanism is arranged on the supporting frame, the carrying plate can be moved horizontally and vertically by the movable carrying mechanism, the carrying plate is flexible and convenient, the carrying plate can be driven to enter and exit by arranging the conveying belt in the incubator body, and the carrying plate is driven by the lifting motor to lift in the incubator body, so that the technical problem of high labor intensity caused by the fact that a culture dish needs to be manually placed in the incubator in the traditional biological antibody culture process is solved;
through placing the loading board in bearing the groove, drive the push pedal through pushing away the material cylinder and remove and drive the loading board and remove, make the culture dish on the loading board pass through the below of drain pipe in proper order, through the on-off control drain pipe on the control nutrition box body to adding the nutrient solution in the culture dish, this biological antibody culture apparatus can carry out the interpolation of nutrient solution to a plurality of culture dishes fast, and is quick convenient, need artificially manually add the culture solution to each culture dish in proper order when having solved traditional biological antibody and having cultivateed, can't guarantee the technical problem of safe clean interpolation environment.
The purpose of the invention can be realized by the following technical scheme:
a biological antibody culture device comprises a culture box body, wherein a feeding and discharging port is formed in one side of the culture box body, a nutrition box body is fixedly arranged at the feeding and discharging port through welding, the nutrition box body is positioned above the feeding and discharging port, a bearing groove is fixedly arranged on one side of the culture box body through bolts and is positioned below the nutrition box body, the notch of the bearing groove is upward, two material pushing cylinders are fixedly arranged at one end, away from the culture box body, of the bearing groove, output rods of the two material pushing cylinders penetrate through the groove wall on one side of the bearing groove and extend into the bearing groove, and push plates are fixedly arranged at the end parts of the output rods of the material pushing cylinders;
the feeding box comprises a feeding box body, a storage bin, a plurality of liquid outlet pipes, a feeding channel and a sealing cover, wherein the storage bin is fixedly arranged on the inner side of the feeding box body, the plurality of liquid outlet pipes are fixedly arranged at the bottom of the storage bin and are distributed side by side at equal intervals, the feeding channel is fixedly connected to the top of the feeding box body and is communicated with the storage bin, and the sealing cover is arranged at the top of the feeding channel;
a belt conveyor for conveying the bearing plate is arranged on one side of the bearing groove, a support frame is arranged above the belt conveyor and the bearing groove, and two movable carrying mechanisms are movably arranged on the support frame along the horizontal direction;
cultivate the inboard level of box and be provided with the backup pad, the backup pad with cultivate the inner wall of box and pass through welded fastening, the upper surface of backup pad has two risers, two along horizontal direction movable mounting the riser is the symmetric distribution, two the equal fixed mounting in inboard of riser has the conveyer belt, the top level of backup pad is provided with the layer board, the bottom surface central point of layer board puts the vertical first threaded rod that is provided with of department, first threaded rod rotates with the layer board to be connected, the below level of backup pad is provided with the motor mounting panel, first threaded rod runs through the backup pad and rotates with the motor mounting panel to be connected, first threaded rod and backup pad threaded connection, elevator motor is installed to the motor mounting panel, elevator motor's output shaft end and the equal fixed mounting in bottom of first threaded rod have the belt pulley, elevator motor's output shaft passes through, The belt is in transmission connection with the first threaded rod;
a second threaded rod is transversely arranged on the upper surface of the supporting plate and is rotatably installed on the upper surface of the supporting plate, the second threaded rod is in threaded connection with the two vertical plates, an adjusting motor is fixedly installed on the bottom surface of the supporting plate, a belt pulley is fixedly installed at the output shaft end of the adjusting motor, a belt pulley is fixedly installed at one end of the second threaded rod, and the output shaft of the adjusting motor is in transmission connection with the second threaded rod through a belt and the belt pulley;
install a plurality of support seat group along vertical direction on the riser, every support seat group includes a plurality of supporting seat, a plurality of the supporting seat is equidistant linear distribution along the horizontal direction, the inboard diapire of supporting seat articulates there is the upset cylinder, the top of supporting seat articulates there is the board that turns over, the one end that turns over the board is articulated with the output rod top of upset cylinder.
Furthermore, the movable carrying mechanism comprises a movable seat, a top plate is horizontally fixed at the top of the movable seat through bolts, a lifting cylinder and a movable motor are fixedly mounted on the upper surface of the top plate, an output shaft of the movable motor penetrates through the top plate and extends to the position below the top plate, a gear is fixedly mounted at the output shaft of the movable motor, an output rod of the lifting cylinder penetrates through the top plate and extends to the position below the top plate, a lifting plate is vertically fixed at the bottom end of the output rod of the lifting cylinder, a sucker mounting plate is horizontally fixed at the bottom end of the lifting plate through welding, four suckers are fixedly mounted at the bottom of the sucker mounting plate, and the four suckers are distributed side by side at equal intervals;
a sliding block is fixedly arranged on one side of the lifting plate, a sliding rail is fixedly arranged on one side of the moving seat, and the lifting plate is connected with the moving seat in a sliding mode through the sliding block and the sliding rail;
a rack is fixedly arranged on one side of the top of the support frame along the horizontal direction and meshed with the gear.
Further, a plurality of culture dish standing grooves have been seted up to the upper surface of loading board, and a plurality of each culture dish standing groove is rectangular array and distributes, and every row of culture dish standing groove is corresponding with the drain pipe.
Furthermore, the turning plates on the two vertical plates are symmetrically distributed.
Furthermore, a second guide rod is fixedly installed on the inner side of the top of the support frame along the horizontal direction, a through hole is formed in the moving seat, and the second guide rod is in sliding fit with the through hole.
Furthermore, the top inside of support frame is along horizontal direction fixed mounting with the slide rail, removes one side fixed mounting of seat and has the slider, it passes through slider, slide rail slidable mounting on the support frame to remove the seat.
Furthermore, the bottom surface of layer board still vertically is fixed with first guide bar, first guide bar is located the both sides of first threaded rod, the bottom of first guide bar and the upper surface fixed connection of motor mounting panel.
Furthermore, the upper surface fixed mounting of backup pad has two slide rails, the bottom fixed mounting of riser has two sliders corresponding with the slide rail, the riser passes through slider, slide rail slidable mounting in the backup pad.
Furthermore, a switch for controlling the liquid outlet pipe is arranged on the nutrition box body.
A culture method of biological antibodies comprises the following specific steps:
step one, putting a culture dish filled with biological antibodies into a culture dish placing groove of a bearing plate, placing the bearing plate on a belt conveyor, starting the belt conveyor, driving the bearing plate to move below a support frame by the belt conveyor, starting a lifting cylinder, descending an output rod of the lifting cylinder and driving a sucker mounting plate to descend, driving a sucker to descend and be in close contact with two side edges of the upper surface of the bearing plate, adsorbing the bearing plate, driving the bearing plate to ascend by the lifting cylinder, starting a moving motor, rotating an output shaft of the moving motor and driving a gear to rotate, enabling the whole moving and carrying mechanism to drive the bearing plate to move above a bearing groove, driving the bearing plate to descend into the bearing groove by the lifting cylinder, and separating the sucker from the bearing plate;
step two, starting a material pushing cylinder, extending an output rod of the material pushing cylinder and driving a push plate to move to one side of the nutrition box body and pushing a bearing plate to move to the bottom of the nutrition box body, opening a liquid outlet pipe when each row of culture dishes on the bearing plate sequentially pass below the liquid outlet pipe, adding nutrient solution in a storage bin into the culture dishes until the nutrient solution in all the culture dishes on the bearing plate is added, and manually pulling out the bearing plate to seal covers of the culture dishes;
step three, push away the material cylinder and promote the loading board and pass the business turn over material mouth and remove on the conveyer belt, drive the top that the loading board removed the layer board through starting the conveyer belt, start elevator motor, elevator motor's output shaft rotates and drives first threaded rod and rotate, and it rises to drive the layer board, the layer board drives the loading board and rises, and make the both sides of loading board remove the top of supporting seat, start upset cylinder, the output pole of upset cylinder stretches out and drives the inboard upset of the one end of turning over the board to the riser, the top that makes the board that turns over is the level form, it descends and makes the loading board place on turning over the board to drive the loading board through elevator motor, repeat above steps, the loading.
The invention has the beneficial effects that:
the multiple culture dish placing grooves are formed in the bearing plate, a single bearing plate can be used for loading multiple culture dishes at the same time, and the multiple bearing plates can be simultaneously accommodated in the culture box body by respectively arranging the multiple layers of supporting seats on the two vertical plates, so that the number of the culture dishes in the culture box body is increased, a large amount of biological antibodies can be simultaneously cultured by the culture box body, the production efficiency of the biological antibodies is improved, and the batch production of the biological antibodies is realized;
the invention transports the bearing plate by a belt conveyor, the movable carrying mechanism is arranged on the support frame, a gear is arranged on an output shaft of a movable motor in the movable carrying mechanism, a rack matched with the gear is arranged on the support frame, the movable motor drives the gear to rotate, the whole movable carrying mechanism can be driven to move, the bearing plate is adsorbed by arranging a sucker on a sucker mounting plate, the bearing plate is convenient to take and place, a lifting cylinder drives a lifting plate to lift so as to drive each sucker to lift, thereby driving the bearing plate to ascend and descend, the movable carrying mechanism can move the bearing plate in the horizontal and vertical directions, the movable carrying mechanism is flexible and convenient, the conveyor belt is arranged in the incubator body, the bearing plate can be driven to enter and exit, the lifting motor drives a first threaded rod to rotate so as to drive a supporting plate to lift, thereby driving the bearing plate to lift in the incubator body, the whole biological culture process adopts an automatic carrying technology, manual carrying is not needed, labor force is saved, and labor intensity is reduced;
through placing the loading board in bearing the groove, it removes and drives the loading board removal to drive the push pedal through pushing away material cylinder, make the culture dish on the loading board pass through the below of drain pipe in proper order, bottom through at the storage silo sets up a plurality of drain pipes, switch control drain pipe on through the control nutrition box adds the nutrient solution in to the culture dish, this biological antibody culture apparatus can carry out the interpolation of nutrient solution to a plurality of culture dishes fast, it is quick convenient, at the interpolation in-process of nutrient solution, operating personnel need not manual contact, safe and clean interpolation environment has been guaranteed, the internal temperature of culture box is adjustable, good cultivation environment is provided for the cultivation of biological antibody.
Drawings
In order to facilitate understanding for those skilled in the art, the present invention will be further described with reference to the accompanying drawings.
FIG. 1 is a perspective view of a biological antibody culture apparatus according to the present invention;
FIG. 2 is a partial structural view of a biological antibody culture apparatus according to the present invention;
FIG. 3 is a sectional view showing the structure of the incubator body according to the present invention;
FIG. 4 is a perspective view showing the internal structure of the incubator body according to the present invention;
FIG. 5 is a perspective view of another angle of the internal structure of the incubator body according to the present invention;
FIG. 6 is a front view showing an internal structure of a incubator body according to the present invention;
FIG. 7 is an assembled cross-sectional view of the support base, the turning plate and the turning cylinder of the present invention;
FIG. 8 is an internal structure view of the nutrition tank of the present invention;
fig. 9 is a perspective view of the moving and conveying mechanism of the present invention.
In the figure: 1. a culture box body; 101. feeding and discharging ports; 2. a support frame; 3. a bearing groove; 4. a material pushing cylinder; 5. pushing the plate; 6. a mobile carrying mechanism; 61. a movable seat; 62. a lifting cylinder; 63. a moving motor; 64. a lifting plate; 65. a sucker mounting plate; 66. a suction cup; 67. a gear; 68. a top plate; 7. a nutrition box body; 8. a belt conveyor; 9. a carrier plate; 10. a vertical plate; 11. a conveyor belt; 12. a motor mounting plate; 13. a lifting motor; 14. a first threaded rod; 15. a first guide bar; 16. a support plate; 17. a support plate; 18. adjusting the motor; 19. a supporting seat; 20. turning over a plate; 21. a second threaded rod; 22. a rack; 23. a storage bin; 24. a liquid outlet pipe; 25. a charging channel; 26. a second guide bar; 27. and (5) turning over the air cylinder.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1-9, a biological antibody culture apparatus includes a culture box body 1, a material inlet and outlet 101 is provided at one side of the culture box body 1, a nutrition box body 7 is fixed at the material inlet and outlet 101 by welding, the nutrition box body 7 is located above the material inlet and outlet 101, a bearing groove 3 is fixedly installed at one side of the culture box body 1 by bolts, the bearing groove 3 is located below the nutrition box body 7, a notch of the bearing groove 3 faces upward, two material pushing cylinders 4 are fixedly installed at one end of the bearing groove 3 far away from the culture box body 1, output rods of the two material pushing cylinders 4 penetrate through a groove wall at one side of the bearing groove 3 and extend into the bearing groove 3, and a push plate 5 is fixedly installed at an end of an output rod of the material pushing cylinder 4;
a storage bin 23 is fixedly mounted on the inner side of the nutrition box body 7, a plurality of liquid outlet pipes 24 are fixedly mounted at the bottom of the storage bin 23, the liquid outlet pipes 24 are distributed side by side at equal intervals, a feeding channel 25 is fixedly connected to the top of the nutrition box body 7, the feeding channel 25 is communicated with the storage bin 23, and a sealing cover is mounted at the top of the feeding channel 25;
a belt conveyor 8 for conveying a bearing plate 9 is arranged on one side of the bearing groove 3, a support frame 2 is arranged above the belt conveyor 8 and the bearing groove 3, and two movable carrying mechanisms 6 are movably arranged on the support frame 2 along the horizontal direction;
the inboard level of cultivateing box 1 is provided with backup pad 17, backup pad 17 pass through welded fastening with the inner wall of cultivateing box 1, the upper surface of backup pad 17 has two risers 10 along horizontal direction movable mounting, two risers 10 are the symmetric distribution, two the equal fixed mounting in inboard of riser 10 has conveyer belt 11, the top level of backup pad 17 is provided with layer board 16, the bottom surface central point department of putting of layer board 16 is vertical to be provided with first threaded rod 14, first threaded rod 14 rotates with layer board 16 to be connected, the below level of backup pad 17 is provided with motor mounting panel 12, first threaded rod 14 runs through backup pad 17 and rotates with motor mounting panel 12 to be connected, first threaded rod 14 and backup pad 17 threaded connection, motor mounting panel 12 installs elevator motor 13, elevator motor 13's the output shaft end and the equal fixed mounting in bottom of first threaded rod 14 have the belt pulley, an output shaft of the lifting motor 13 is in transmission connection with the first threaded rod 14 through a belt pulley and a belt;
a second threaded rod 21 is transversely arranged on the upper surface of the supporting plate 17, the second threaded rod 21 is rotatably mounted on the upper surface of the supporting plate 17, the second threaded rod 21 is in threaded connection with the two vertical plates 10, an adjusting motor 18 is fixedly mounted on the bottom surface of the supporting plate 17, a belt pulley is fixedly mounted at an output shaft of the adjusting motor 18, a belt pulley is fixedly mounted at one end of the second threaded rod 21, and an output shaft of the adjusting motor 18 is in transmission connection with the second threaded rod 21 through a belt and the belt pulley;
install a plurality of support seat group along vertical direction on the riser 10, every support seat group includes a plurality of supporting seat 19, a plurality of supporting seat 19 is equidistant linear distribution along the horizontal direction, the inboard diapire of supporting seat 19 articulates there is upset cylinder 27, the top of supporting seat 19 articulates there is board 20 that turns over, the one end that turns over board 20 is articulated with the output lever top of upset cylinder 27.
The movable carrying mechanism 6 comprises a movable base 61, a top plate 68 is horizontally fixed at the top of the movable base 61 through a bolt, a lifting cylinder 62 and a movable motor 63 are fixedly installed on the upper surface of the top plate 68, an output shaft of the movable motor 63 penetrates through the top plate 68 and extends to the lower part of the top plate 68, a gear 67 is fixedly installed at the output shaft end of the movable motor 63, an output rod of the lifting cylinder 62 penetrates through the top plate 68 and extends to the lower part of the top plate 68, a lifting plate 64 is vertically fixed at the bottom end of the output rod of the lifting cylinder 62, a sucker mounting plate 65 is horizontally fixed at the bottom end of the lifting plate 64 through welding, four suckers 66 are fixedly installed at the bottom of the sucker mounting plate 65, and the four suckers 66 are distributed side by side at equal intervals;
a sliding block is fixedly installed on one side of the lifting plate 64, a sliding rail is fixedly installed on one side of the moving seat 61, and the lifting plate 64 is connected with the moving seat 61 in a sliding mode through the sliding block and the sliding rail;
a rack 22 is fixedly installed on one side of the top of the support frame 2 along the horizontal direction, and the rack 22 is meshed with a gear 67.
A plurality of culture dish standing grooves are seted up to the upper surface of loading board 9, and a plurality of each culture dish standing groove is rectangular array and distributes, and every row of culture dish standing groove is corresponding with drain pipe 24.
The turning plates 20 on the two vertical plates 10 are symmetrically distributed.
The inboard horizontal direction fixed mounting of top of support frame 2 has second guide bar 26, has seted up the through-hole on the removal seat 61, second guide bar 26 and through-hole sliding fit.
The top inboard of support frame 2 has a slide rail along horizontal direction fixed mounting, and one side fixed mounting who removes seat 61 has the slider, it passes through slider, slide rail slidable mounting on support frame 2 to remove seat 61.
A first guide rod 15 is vertically fixed on the bottom surface of the supporting plate 16, the first guide rods 15 are located on two sides of the first threaded rod 14, and the bottom end of the first guide rod 15 is fixedly connected with the upper surface of the motor mounting plate 12.
The last fixed surface of backup pad 17 installs two slide rails, the bottom fixed mounting of riser 10 has two sliders corresponding with the slide rail, riser 10 passes through slider, slide rail slidable mounting on backup pad 17.
And a switch for controlling the liquid outlet pipe 24 is arranged on the nutrition box body 7.
A culture method of biological antibodies comprises the following specific steps:
step one, putting a culture dish filled with biological antibodies into a culture dish placing groove of a bearing plate 9, putting the bearing plate 9 on a belt conveyor 8, starting the belt conveyor 8, driving the bearing plate 9 to move to the lower part of a support frame 2 by the belt conveyor 8, starting a lifting cylinder 62, descending an output rod of the lifting cylinder 62 and driving a sucker mounting plate 65 to descend, driving a sucker 66 to descend to be in close contact with two side edges of the upper surface of the bearing plate 9, adsorbing the bearing plate 9, driving the bearing plate 9 to ascend by the lifting cylinder 62, starting a moving motor 63, rotating an output shaft of the moving motor 63 and driving a gear 67 to rotate, so that the whole moving and carrying mechanism 6 drives the bearing plate 9 to move to the upper part of a bearing groove 3, driving the bearing plate 9 to descend to the bearing groove 3 by the lifting cylinder 62, and separating the sucker 66 from the bearing plate 9;
step two, starting the material pushing cylinder 4, extending an output rod of the material pushing cylinder 4 and driving the push plate 5 to move towards one side of the nutrition box body 7, and pushing the bearing plate 9 to move to the bottom of the nutrition box body 7, wherein when each row of culture dishes on the bearing plate 9 sequentially pass below the liquid outlet pipe 24, the liquid outlet pipe 24 is opened, nutrient solution in the storage bin 23 is added into the culture dishes, and the bearing plate 9 is manually pulled out to seal the culture dishes until the nutrient solution in all the culture dishes on the bearing plate 9 is completely added;
step three, the material pushing cylinder 4 pushes the bearing plate 9 to penetrate through the material inlet and outlet 101 and move to the conveyor belt 11, the conveyor belt 11 is started to drive the bearing plate 9 to move to the position above the supporting plate 16, the lifting motor 13 is started, the output shaft of the lifting motor 13 rotates and drives the first threaded rod 14 to rotate and drive the supporting plate 16 to ascend, the supporting plate 16 drives the bearing plate 9 to ascend, the two sides of the bearing plate 9 move to the position above the supporting seat 19, the overturning cylinder 27 is started, the output rod of the overturning cylinder 27 extends out and drives one end of the overturning plate 20 to overturn towards the inner side of the vertical plate 10, the top of the overturning plate 20 is made to be horizontal, the bearing plate 9 is driven to descend through the lifting motor 13 and the bearing plate 9 is placed on the overturning plate 20, and.
According to the invention, the plurality of culture dish placing grooves are formed in the bearing plate 9, a plurality of culture dishes can be loaded on a single bearing plate 9 at the same time, and the plurality of bearing plates 9 can be accommodated in the culture box body 1 at the same time by arranging the plurality of layers of supporting seats 19 on the two vertical plates 10 respectively, so that the number of the culture dishes in the culture box body 1 is increased, a large amount of biological antibodies can be cultured in the culture box body 1 at the same time, the production efficiency of the biological antibodies is improved, and the batch production of the biological antibodies is realized;
the invention transports the bearing plate 9 by the belt conveyer 8, the moving and carrying mechanism 6 is arranged on the support frame 2, the gear 67 is arranged on the output shaft of the moving motor 63 in the moving and carrying mechanism 6, the rack 22 matched with the gear 67 is arranged on the support frame 2, the gear 67 is driven by the moving motor 63 to rotate, the whole moving and carrying mechanism 6 can be driven to move, the bearing plate 9 is adsorbed by the sucking disc 66 arranged on the sucking disc mounting plate 65, the bearing plate 9 is convenient to take and place, the lifting cylinder 62 drives the lifting plate 64 to lift so as to drive each sucking disc 66 to lift, thereby driving the lifting and falling of the bearing plate 9, the bearing plate 9 can be moved horizontally and vertically by the moving and carrying mechanism 6, the invention is flexible and convenient, the bearing plate 9 can be driven to enter and exit by arranging the conveyer belt 11 in the culture box body 1, the lifting motor 13 drives the first threaded rod 14 to rotate so as to drive the supporting plate 16 to lift, so that the bearing plate 9 is driven to lift in the culture box body 1, an automatic carrying technology is adopted in the whole biological culture process, manual carrying is not needed, labor force is saved, and meanwhile, the labor intensity is reduced;
through placing loading board 9 in bearing groove 3, drive push pedal 5 through pushing away material cylinder 4 and remove and drive loading board 9 and remove, make the culture dish on the loading board 9 pass through drain pipe 24's below in proper order, bottom through at storage silo 23 sets up a plurality of drain pipes 24, switch control drain pipe 24 on through control nutrition box 7 adds the nutrient solution to in the culture dish, this biological antibody culture apparatus can carry out the interpolation of nutrient solution to a plurality of culture dishes fast, it is quick convenient, at the interpolation in-process of nutrient solution, operating personnel need not manual contact, safe clean interpolation environment has been guaranteed, 1 inside temperature of culture box is adjustable, good cultivation environment is provided for the cultivation of biological antibody.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (6)
1. A biological antibody culture device is characterized by comprising a culture box body (1), a feed and discharge port (101) is formed in one side of the culture box body (1), the feed and discharge port (101) is fixedly provided with a nutrition box body (7) through welding, the nutrition box body (7) is positioned above the feed inlet and the discharge outlet (101), one side of the culture box body (1) is fixedly provided with a bearing groove (3) through a bolt, the bearing groove (3) is positioned below the nutrition box body (7), the notch of the bearing groove (3) is upward, two material pushing cylinders (4) are fixedly arranged at one end of the bearing groove (3) far away from the culture box body (1), output rods of the two material pushing cylinders (4) penetrate through the groove wall at one side of the bearing groove (3) and extend into the bearing groove (3), a push plate (5) is fixedly arranged at the end part of an output rod of the material pushing cylinder (4);
a storage bin (23) is fixedly mounted on the inner side of the nutrition box body (7), a plurality of liquid outlet pipes (24) are fixedly mounted at the bottom of the storage bin (23), the liquid outlet pipes (24) are distributed side by side at equal intervals, a feeding channel (25) is fixedly connected to the top of the nutrition box body (7), the feeding channel (25) is communicated with the storage bin (23), and a sealing cover is mounted at the top of the feeding channel (25);
a belt conveyor (8) for conveying a bearing plate (9) is arranged on one side of the bearing groove (3), a support frame (2) is arranged above the belt conveyor (8) and the bearing groove (3), and two moving and carrying mechanisms (6) are movably arranged on the support frame (2) along the horizontal direction;
the inner side of the culture box body (1) is horizontally provided with a support plate (17), the inner wall of the support plate (17) and the culture box body (1) is fixed by welding, the upper surface of the support plate (17) is movably provided with two vertical plates (10) along the horizontal direction, the two vertical plates (10) are symmetrically distributed, the inner sides of the two vertical plates (10) are fixedly provided with a conveyor belt (11), the upper side of the support plate (17) is horizontally provided with a support plate (16), the central position of the bottom surface of the support plate (16) is vertically provided with a first threaded rod (14), the first threaded rod (14) is rotatably connected with the support plate (16), the lower side of the support plate (17) is horizontally provided with a motor mounting plate (12), the first threaded rod (14) penetrates through the support plate (17) and is rotatably connected with the motor mounting plate (12), and the first threaded rod (14) is in threaded connection with, the motor mounting plate (12) is provided with a lifting motor (13), belt pulleys are fixedly mounted at the output shaft end of the lifting motor (13) and the bottom end of the first threaded rod (14), and the output shaft of the lifting motor (13) is in transmission connection with the first threaded rod (14) through the belt pulleys and the belt;
the upper surface of the supporting plate (17) is transversely provided with a second threaded rod (21), the second threaded rod (21) is rotatably installed on the upper surface of the supporting plate (17), the second threaded rod (21) is in threaded connection with the two vertical plates (10), the bottom surface of the supporting plate (17) is fixedly provided with an adjusting motor (18), the output shaft of the adjusting motor (18) is fixedly provided with a belt pulley, one end of the second threaded rod (21) is fixedly provided with a belt pulley, and the output shaft of the adjusting motor (18) is in transmission connection with the second threaded rod (21) through a belt and the belt pulley;
a plurality of supporting seat groups are arranged on the vertical plate (10) along the vertical direction, each supporting seat group comprises a plurality of supporting seats (19), the supporting seats (19) are linearly distributed at equal intervals along the horizontal direction, the bottom wall of the inner side of each supporting seat (19) is hinged with a turning cylinder (27), the top of each supporting seat (19) is hinged with a turning plate (20), and one end of each turning plate (20) is hinged with the top end of an output rod of each turning cylinder (27);
the moving and carrying mechanism (6) comprises a moving seat (61), a top plate (68) is horizontally fixed at the top of the moving seat (61) through a bolt, the upper surface of the top plate (68) is fixedly provided with a lifting cylinder (62) and a moving motor (63), the output shaft of the moving motor (63) penetrates through the top plate (68) and extends to the lower part of the top plate (68), and a gear (67) is fixedly arranged at the output shaft end of the moving motor (63), the output rod of the lifting cylinder (62) penetrates through the top plate (68) and extends to the lower part of the top plate (68), and a lifting plate (64) is vertically fixed at the bottom end of an output rod of the lifting cylinder (62), a sucker mounting plate (65) is horizontally fixed at the bottom end of the lifting plate (64) by welding, the bottom of the sucker mounting plate (65) is fixedly provided with four suckers (66), and the four suckers (66) are distributed side by side at equal intervals;
a sliding block is fixedly installed on one side of the lifting plate (64), a sliding rail is fixedly installed on one side of the moving seat (61), and the lifting plate (64) is connected with the moving seat (61) in a sliding mode through the sliding block and the sliding rail;
a rack (22) is fixedly installed on one side of the top of the support frame (2) along the horizontal direction, and the rack (22) is meshed with a gear (67);
the upper surface of the bearing plate (9) is provided with a plurality of culture dish placing grooves, the culture dish placing grooves are distributed in a rectangular array, and each row of culture dish placing grooves correspond to the liquid outlet pipe (24);
a second guide rod (26) is fixedly installed on the inner side of the top of the support frame (2) along the horizontal direction, a through hole is formed in the moving seat (61), and the second guide rod (26) is in sliding fit with the through hole;
the top inboard of support frame (2) has the slide rail along horizontal direction fixed mounting, and one side fixed mounting who removes seat (61) has the slider, remove seat (61) and pass through slider, slide rail slidable mounting on support frame (2).
2. A biological antibody culture device according to claim 1, characterised in that the flaps (20) on the two risers (10) are symmetrically distributed.
3. The biological antibody culture device according to claim 1, wherein a first guide rod (15) is vertically fixed on the bottom surface of the supporting plate (16), the first guide rod (15) is positioned on two sides of the first threaded rod (14), and the bottom end of the first guide rod (15) is fixedly connected with the upper surface of the motor mounting plate (12).
4. The biological antibody culture device according to claim 1, wherein two sliding rails are fixedly installed on the upper surface of the supporting plate (17), two sliding blocks corresponding to the sliding rails are fixedly installed on the bottom of the vertical plate (10), and the vertical plate (10) is slidably installed on the supporting plate (17) through the sliding blocks and the sliding rails.
5. A biological antibody culture apparatus according to claim 1, characterized in that the nutrition box (7) is provided with a switch for controlling the liquid outlet pipe (24).
6. A culture method for the biological antibody of claims 1-5, which comprises the following steps:
step one, putting a culture dish filled with biological antibodies into a culture dish placing groove of a bearing plate (9), placing the bearing plate (9) on a belt conveyor (8), starting the belt conveyor (8), driving the bearing plate (9) to move to the lower part of a support frame (2) by the belt conveyor (8), starting a lifting cylinder (62), descending an output rod of the lifting cylinder (62) and driving a sucker mounting plate (65) to descend, driving a sucker (66) to descend to be in close contact with two side edges of the upper surface of the bearing plate (9), adsorbing the bearing plate (9), driving the bearing plate (9) to ascend by the lifting cylinder (62), starting a moving motor (63), rotating an output shaft of the moving motor (63) and driving a gear (67) to rotate, so that the whole moving and carrying mechanism (6) drives the bearing plate (9) to move to the upper part of a bearing groove (3), the bearing plate (9) is driven to descend into the bearing groove (3) through the lifting cylinder (62), and the sucker (66) is separated from the bearing plate (9);
step two, starting the material pushing cylinder (4), enabling an output rod of the material pushing cylinder (4) to extend out and drive the push plate (5) to move towards one side of the nutrition box body (7), pushing the bearing plate (9) to move to the bottom of the nutrition box body (7), opening the drain pipe (24) when each row of culture dishes on the bearing plate (9) sequentially pass through the lower part of the drain pipe (24), adding nutrient solution in the storage bin (23) into the culture dishes until the nutrient solution in all the culture dishes on the bearing plate (9) is completely added, and manually pulling out the bearing plate (9) to seal covers of the culture dishes;
step three, the material pushing cylinder (4) pushes the bearing plate (9) to penetrate through the material inlet and outlet (101) and move to the conveyor belt (11), the conveyor belt (11) is started to drive the bearing plate (9) to move to the position above the supporting plate (16), the lifting motor (13) is started, an output shaft of the lifting motor (13) rotates and drives the first threaded rod (14) to rotate and drive the supporting plate (16) to ascend, the supporting plate (16) drives the bearing plate (9) to ascend, two sides of the bearing plate (9) move to the position above the supporting seat (19), the overturning cylinder (27) is started, an output rod of the overturning cylinder (27) extends out and drives one end of the overturning plate (20) to overturn towards the inner side of the vertical plate (10), the top of the overturning plate (20) is made to be horizontal, the bearing plate (9) is driven to descend by the lifting motor (13) and the bearing plate (9) is placed on the, until the bearing plates (9) are arranged on the turning plates (20) of each layer.
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| CN202010152651.8A CN111304082B (en) | 2020-03-06 | 2020-03-06 | Biological antibody culture device and culture method |
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| CN114214193B (en) * | 2021-12-06 | 2023-07-25 | 中南大学湘雅医院 | Culture device for treating osteoarthritis |
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| CN201046960Y (en) * | 2007-02-13 | 2008-04-16 | 南京林业大学 | Pallet translation apparatus |
| EP3269798A1 (en) * | 2015-03-31 | 2018-01-17 | Dokkyo Medical University | Incubator provided with clean bench function, and incubator system |
| CN206428232U (en) * | 2017-01-22 | 2017-08-22 | 杭州聚立医疗用品有限公司 | Culture dish takes ware device automatically |
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Address after: 239000 No.69, qifushi West Road, Chuzhou economic and Technological Development Zone, Anhui Province Patentee after: General Biology (Anhui) Co.,Ltd. Address before: 239000 No.6 yongyang Road, Chuzhou economic and Technological Development Zone, Anhui Province Patentee before: GENERAL BIOSYSTEMS (ANHUI), Inc. |