CN111249346A - Preparation and use method of compound origanum oral liquid for treating piglet diarrhea - Google Patents

Abstract

The invention relates to the field of veterinary drugs, in particular to a preparation method and a use method of a compound origanum oral liquid for treating piglet diarrhea.

Description

Preparation and use method of compound origanum oral liquid for treating piglet diarrhea

Technical Field

The invention relates to the field of veterinary drugs, and in particular relates to a preparation method and a use method of compound oregano oral liquid for treating piglet diarrhea.

Background

Origanum is commonly known as dysentery stopping grass, marjoram, hyssop, elsholtzia and the like, is a perennial herb of origanum in the family of labiatae, and mainly grows in europe, northern africa, asia and other areas. In China, oregano is mainly distributed in places such as Sichuan, Yunnan, Gansu, Hubei, Jiangxi, Anhui, Guizhou and the like, and originates from hillside, roadside, shrubs, grasslands and forests with the altitude of 500-3600 m.

The father of Western medicine, Hibocela, found that oregano has the effects of sterilization, antibiosis, relieving gastrointestinal discomfort and respiratory problems, and the like. In the middle ages oregano has been used as an antimicrobial and food preservative. The oregano oil of the 17 th century is used for treating cold. In recent times, oregano is widely used for cooking gourmet food.

The origanum vulgare is pungent in taste, cool in nature and nontoxic, aromatic oil can be extracted from the whole herb, and the origanum vulgare can also be used as an aromatic oil, can also be used as a honey source plant, and has the effects of clearing heat, relieving exterior syndrome, regulating qi, eliminating dampness, inducing diuresis and relieving swelling.

The oregano oil is volatile oil with special aromatic odor extracted from oregano grass, is transparent, has light yellow color, and is a mixture. Modern pharmacological studies show that the oregano oil has the effects of inhibiting bacteria, resisting viruses, resisting oxidation, enhancing the immunity of the organism, aiding digestion and the like. The origanum oil as a novel natural plant antibiotic has the characteristics of wide source, obvious effect, difficult generation of drug resistance, no residue and the like compared with the traditional antibiotic. Oregano oil is a pharmaceutical additive approved by the Ministry of agriculture (Nongsufa No. 200l 20) for use in a few cases that do not require withdrawal periods. The high-quality natural oregano essential oil has wide market prospect both at home and abroad.

But at present, no standard for the oregano oil product exists in China. The foreign oregano oil products are mostly extracted from pure nature, have various active ingredients, high content and reliable product quality, and can be used for prevention and treatment. Most of domestic oregano essential oil products are synthesized by chemical engineering, the active ingredients are single, and the actual application effect is unclear.

Piglet diarrhea is a typical multifactorial disease. The types of pathogens causing piglet diarrhea are various and the disease property is complex. Piglet diarrhea has long been a significant cause of impaired piglet growth and high piglet mortality. Diarrhea in piglets can lead to low feed conversion rates, slow or arrested growth (runt pigs), decreased survival rate of piglets, and even death. The diarrhea of the piglets brings billions of yuan of direct economic loss to the pig industry every year.

Disclosure of Invention

The invention aims to overcome the defects in the prior art and provides a preparation method and a using method of a compound origanum oral liquid for treating piglet diarrhea.

The invention is realized by the following technical scheme:

a preparation method of a compound origanum oral liquid for treating piglet diarrhea comprises the following steps,

weighing 50-100 parts of origanum vulgaris, 30-70 parts of coptis chinensis, 30-70 parts of folium artemisiae argyi, 20-70 parts of radix sophorae flavescentis and 300 parts of elsholtzia haichowensis by weight, respectively cleaning, drying, crushing to 300 meshes, and mixing to obtain mixed traditional Chinese medicine powder;

step two, putting the mixed traditional Chinese medicine powder into 8-12 times of water for soaking for 15-45min, treating with ultrasonic waves with the frequency of 40-80Hz during the soaking, then distilling twice, distilling for 2h each time, collecting the distillate twice, cooling to room temperature, filtering, adding 1-2% of Tween 80 by weight, standing to obtain clear distillate, and dispersing the components of the liquid medicine more uniformly through vibration treatment of the ultrasonic waves;

filtering the decoction obtained after twice distillation, concentrating the filtrate until the relative density is 1.2-1.25, adding ethanol with the volume of 1.5 times of the volume of the filtrate, standing the filtrate for 24 hours, and taking supernatant to obtain alcohol-soluble substance;

and step four, mixing the clear distillate and the alcohol solution, adjusting the pH value to 4.1-5.5, adding sorbic acid, sterilizing at 100 ℃ for 20min, filling to obtain a finished compound origanum oral liquid for treating piglet diarrhea, and refrigerating for later use.

Furthermore, the raw material of the oregano is obtained from wild domesticated oregano of Dabie mountain or Funiu mountain.

Further, in the first step, 80 parts of origanum vulgaris, 50 parts of coptis chinensis, 50 parts of folium artemisiae argyi, 40 parts of radix sophorae flavescentis and 200 parts of elsholtzia are weighed.

Further, in the second step, the temperature of the two distillations is 80-100 ℃, the interval between the two distillations is 15-45min, the essential oil of the main effective components of origanum vulgaris, folium artemisiae argyi and elsholtzia can be obtained by the two distillations, and the distillation temperature is set at 95 ℃ in the production process.

Further, in the third step, a 5um filter membrane is adopted for filtration.

Further, in step four, the pH was adjusted by sodium phosphate buffer.

Further, a use method of the compound oregano oral liquid for treating piglet diarrhea is used for treating piglet diarrhea, and comprises the following steps:

the compound origanum oral liquid for treating piglet diarrhea is orally taken by piglets with diarrhea, and is taken once a day with 2-4ml per head.

The invention has the beneficial effects that:

coptis chinensis: has effects of clearing heat, eliminating dampness, purging pathogenic fire, and removing toxic substance;

folium artemisiae argyi: warming channels to stop bleeding, dispelling cold to relieve pain, eliminating dampness to relieve itching;

flavescent sophora root: clearing heat, eliminating dampness, killing parasites and promoting urination;

herba Moslae: induce sweating to relieve exterior syndrome, resolve dampness and harmonize the middle warmer, induce diuresis to alleviate edema;

potassium sorbate: a preservative which can prolong the shelf life;

tween 80: used as a co-solvent.

1. The traditional Chinese medicine preparation has no toxic or side effect, is safe and reliable to use, and does not generate medicine residue and social public nuisance.

2. The pure natural origanum essential oil is extracted, which is the first time in China. The origanum essential oil has the pharmacological effects of inhibiting bacteria, resisting virus, resisting oxidation, enhancing immunity and promoting digestion. The five medicines of origanum vulgaris, coptis chinensis, folium artemisiae argyi, radix sophorae flavescentis and elsholtzia moschata act synergistically, and the antibacterial and antiviral effects of the oral liquid are greatly improved.

3. The feed mainly treats infectious diarrhea and non-infectious diarrhea of piglets, can enhance the immunity of piglets, promote the digestion of piglets, improve the survival rate of piglets and the feed reward, and increase the breeding benefit.

4. In the whole preparation process of the invention, the produced waste does not pollute the environment and is easy to treat.

5. The invention adopts the production process of extracting the traditional Chinese medicine to produce, extracts the effective components of the traditional Chinese medicine to the maximum extent and well removes impurities in the traditional Chinese medicine extracting solution.

Detailed Description

The present invention will be described below with reference to specific examples.

The raw material of the oregano is selected from wild oregano domesticated species of Dabie mountain or Funiu mountain, and is specifically wild oregano domesticated species provided by Bingqi Chinese medicinal plant professional cooperative society in Nanyang city.

In the second step, the temperature of the two times of distillation is 80-100 ℃, and the interval between the two times of distillation is 15-45 min.

In the second step and the third step, a 5um filter membrane is adopted for filtration.

In step four, the pH is adjusted by sodium phosphate buffer.

Example 1

A preparation method of a compound origanum oral liquid for treating piglet diarrhea comprises the following steps,

weighing 80Kg of origanum vulgaris, 50 Kg of coptis chinensis, 50 Kg of folium artemisiae argyi, 40 Kg of radix sophorae flavescentis and 200 Kg of elsholtzia, respectively cleaning, drying, crushing to 300 meshes, and mixing to obtain mixed traditional Chinese medicine powder;

step two, putting the mixed traditional Chinese medicine powder into 8-12 times of water for soaking for 15-45min, treating with ultrasonic waves with the frequency of 40-80Hz during the soaking, then distilling twice, distilling for 2h each time, collecting the distillate twice, cooling to room temperature, filtering, adding 1-2% of Tween 80 by weight, and standing to obtain clear distillate;

filtering the decoction obtained after twice distillation, concentrating the filtrate until the relative density is 1.2-1.25, adding ethanol with the volume of 1.5 times of the volume of the filtrate, standing the filtrate for 24 hours, and taking supernatant to obtain alcohol-soluble substance;

and step four, mixing the clear distillate and the alcohol solution, adjusting the pH value to 4.1-5.5, adding sorbic acid, sterilizing at 100 ℃ for 20min, filling to obtain a finished compound origanum oral liquid for treating piglet diarrhea, and refrigerating for later use.

Example 2

A preparation method of a compound origanum oral liquid for treating piglet diarrhea comprises the following steps,

weighing the following raw materials, namely 100Kg of origanum vulgaris, 60Kg of coptis chinensis, 60Kg of folium artemisiae argyi, 50 Kg of radix sophorae flavescentis and 250 Kg of elsholtzia, respectively cleaning, drying, crushing to 300 meshes, and mixing to obtain mixed traditional Chinese medicine powder;

step two, putting the mixed traditional Chinese medicine powder into 8-12 times of water for soaking for 15-45min, treating with ultrasonic waves with the frequency of 40-80Hz during the soaking, then distilling twice, distilling for 2h each time, collecting the distillate twice, cooling to room temperature, filtering, adding 1-2% of Tween 80 by weight, and standing to obtain clear distillate;

filtering the decoction obtained after twice distillation, concentrating the filtrate until the relative density is 1.2-1.25, adding ethanol with the volume of 1.5 times of the volume of the filtrate, standing the filtrate for 24 hours, and taking supernatant to obtain alcohol-soluble substance;

and step four, mixing the clear distillate and the alcohol solution, adjusting the pH value to 4.1-5.5, adding sorbic acid, sterilizing at 100 ℃ for 20min, filling to obtain a finished compound origanum oral liquid for treating piglet diarrhea, and refrigerating for later use.

Example 3

A preparation method of a compound origanum oral liquid for treating piglet diarrhea comprises the following steps,

weighing 60Kg of origanum vulgaris, 60Kg of coptis chinensis, 60Kg of folium artemisiae argyi, 30 Kg of radix sophorae flavescentis and 180 Kg of elsholtzia, respectively cleaning, drying, crushing to 300 meshes, and mixing to obtain mixed traditional Chinese medicine powder;

step two, putting the mixed traditional Chinese medicine powder into 8-12 times of water for soaking for 15-45min, treating with ultrasonic waves with the frequency of 40-80Hz during the soaking, then distilling twice, distilling for 2h each time, collecting the distillate twice, cooling to room temperature, filtering, adding 1-2% of Tween 80 by weight, and standing to obtain clear distillate;

filtering the decoction obtained after twice distillation, concentrating the filtrate until the relative density is 1.2-1.25, adding ethanol with the volume of 1.5 times of the volume of the filtrate, standing the filtrate for 24 hours, and taking supernatant to obtain alcohol-soluble substance;

and step four, mixing the clear distillate and the alcohol solution, adjusting the pH value to 4.1-5.5, adding sorbic acid, sterilizing at 100 ℃ for 20min, filling to obtain a finished compound origanum oral liquid for treating piglet diarrhea, and refrigerating for later use.

Example 4

A preparation method of a compound origanum oral liquid for treating piglet diarrhea comprises the following steps,

weighing 120Kg of origanum vulgaris, 90 Kg of coptis chinensis, 90 Kg of folium artemisiae argyi, 70 Kg of radix sophorae flavescentis and 270 Kg of elsholtzia, respectively cleaning, drying, crushing to 300 meshes, and mixing to obtain mixed traditional Chinese medicine powder;

step two, putting the mixed traditional Chinese medicine powder into 8-12 times of water for soaking for 15-45min, treating with ultrasonic waves with the frequency of 40-80Hz during the soaking, then distilling twice, distilling for 2h each time, collecting the distillate twice, cooling to room temperature, filtering, adding 1-2% of Tween 80 by weight, and standing to obtain clear distillate;

filtering the decoction obtained after twice distillation, concentrating the filtrate until the relative density is 1.2-1.25, adding ethanol with the volume of 1.5 times of the volume of the filtrate, standing the filtrate for 24 hours, and taking supernatant to obtain alcohol-soluble substance;

and step four, mixing the clear distillate and the alcohol solution, adjusting the pH value to 4.1-5.5, adding sorbic acid, sterilizing at 100 ℃ for 20min, filling to obtain a finished compound origanum oral liquid for treating piglet diarrhea, and refrigerating for later use.

A use method of the compound oregano oral liquid for treating piglet diarrhea is used for treating piglet diarrhea, the compound oregano oral liquid for treating piglet diarrhea obtained in examples 1-4 is orally administered to diarrhea piglets, the administration amount is 2-4 ml/head, and the administration is performed once a day.

To verify the effect, the experiments were designed as follows,

the sick piglet has the following symptoms: mental retardation, yellow-to-paste discharge, yellowish-to-white watery discharge, whitish paste, pasty discharge, fishy feces, anal laxity, fecal incontinence, soiling of the tail, perineum and hind legs, and reddish coloration of the anus and vulva.

100 diarrhea piglets are selected, the age of the piglets is about 28 days, and the weights of the piglets are close. The diarrhea piglets were equally divided into A, B, C, D four groups. Group A was blank and fed with distilled water. And B group is fed with the compound oregano oral liquid for treating piglet diarrhea obtained in the example 1, C group is fed with the compound oregano oral liquid for treating piglet diarrhea obtained in the example 2, and D group is enrofloxacin group, and the piglets are fed according to the enrofloxacin use standard. After feeding for 10 days, the weight and the feces score of piglets are counted, the weight change is shown in an attached table 1, the feces score standard is shown in an attached table 2, and the feces score is shown in an attached table 3.

Attached table 1

In attached table 1, the differences among those who are the same as the column shoulder mark lowercase letters are not significant (P > 0.05); the difference is obvious when the same row of shoulder marks are different from lower case letters (P < 0.05); the difference is very significant for those with capital letters in the same row (P < 0.01).

The weights of the piglets in the four groups of piglets are not obviously different (P > 0.05), which indicates that the groups of the piglets are correct. Ten days after the diarrhea piglets were fed, the average body weight and average gain weight of B, C, D groups were higher than those of group a, and the difference was extremely significant (P < 0.01). Wherein the average body weight and the average weight gain of piglets in the group B are higher than those in the C, D groups, and the difference is significant (P < 0.05).

Attached table 2

Attached table 3

In attached table 3, after the diarrhea piglets are fed, the fecal scores of B, C, D three groups are all lower than that of the A group, and the fecal scores of the B group are very different from those of the A group (P is less than 0.01). Group B had fecal scores below C, D.

The difference is not obvious when the same column of shoulder mark lower case letters are the same (P > 0.05); the difference is obvious when the same row of shoulder marks are different from lower case letters (P < 0.05); the difference is very significant for those with capital letters in the same row (P < 0.01).

The treatment principle of the compound oregano oral liquid for treating piglet diarrhea obtained by the application comprises the following two points: the natural origanum essential oil has antibacterial ability and is not easy to generate drug resistance. Considering that plant essential oils contain a large number of chemical components of different structures, their antibacterial properties cannot be attributed to one mechanism, but rather have several different target sites for cells. (1) An important mechanism for inhibiting bacteria of plant essential oil is to utilize the hydrophobicity of the plant essential oil to separate lipid on the bacterial cell wall and mitochondria, interfere the structure of the plant essential oil and increase the permeability of the plant essential oil. (2) The main component of phenolic substances in oregano oil can change the permeability of cell membrane by denaturing protein in cell membrane, or react with phospholipid in cell membrane, to destroy protein synthesis, so that the growth of microbial cell is inhibited. (3) Terpenoids, the main component of oregano oil, can also affect the self-replication process of DNA in bacteria, thereby inhibiting the propagation and growth process of bacteria and further inhibiting the growth of bacteria.

Since oregano essential oil is a volatile oil, in the second step of actual production, distillation is performed twice to extract distillate, which is actually an extraction process of oregano essential oil from oregano. The process can effectively improve the oil yield of the oregano oil.

Secondly, the method comprises the following steps: according to the pathogenesis characteristics of diarrhea due to spleen deficiency and excessive dampness and spleen failure in transportation, the treatment should be based on the principle of transporting spleen and clearing dampness. For exterior pathogen, summer-heat pathogen and food stagnation, they are combined with exterior-dispersing, summer-heat-clearing and food-stagnation-relieving herbs respectively. In the prescription, the three medicinal materials of the coptis chinensis, the folium artemisiae argyi and the radix sophorae flavescentis all have the effect of drying dampness, and the difference is that the coptis chinensis and the radix sophorae flavescentis can purge fire and remove toxin while drying dampness, and have the effects of treating damp-heat and summer-heat evil; the folium artemisiae argyi can warm the channel and stop bleeding while drying dampness, has obvious effect on treating downward flow of cold-dampness, and can also eliminate diarrhea and food stagnation; herba Moslae can resolve dampness and regulate the middle warmer, and its sweating and exterior-releasing functions can be well used for treating diarrhea and exterior syndrome. Herba Moslae and radix Sophorae Flavescentis also have diuretic effect, and can promote the discharge of dampness from waterway. The medicines are cooperated to effectively treat diarrhea.

In the medical process, the volatile oil of the folium artemisiae argyi and the effective components of the elsholtzia are taken, and the distillate can be obtained by distillation in the second step. The active ingredients of the coptis and the sophora are mostly in the decoction, and impurities can be removed through the extraction in the second step and the extraction in the third step. The yield can be improved by respectively extracting the volatile oil and the decoction, and the active ingredients of each medicinal material can be furthest reserved according to different properties of the active ingredients.

Meanwhile, the compatibility of the medicines can also improve the drug effect.

If the piglets and the sows have diarrhea at the same time, the administration can be carried out at the same time.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.

Claims (7)

1. A preparation method of a compound origanum oral liquid for treating piglet diarrhea is characterized by comprising the following steps,

weighing 50-100 parts of origanum vulgaris, 30-70 parts of coptis chinensis, 30-70 parts of folium artemisiae argyi, 20-70 parts of radix sophorae flavescentis and 300 parts of elsholtzia haichowensis by weight, respectively cleaning, drying, crushing to 300 meshes, and mixing to obtain mixed traditional Chinese medicine powder;

step two, putting the mixed traditional Chinese medicine powder into 8-12 times of water for soaking for 15-45min, treating with ultrasonic waves with the frequency of 40-80Hz during the soaking, then distilling twice, distilling for 2h each time, collecting the distillate twice, cooling to room temperature, filtering, adding 1-2% of Tween 80 by weight, and standing to obtain clear distillate;

filtering the decoction obtained after twice distillation, concentrating the filtrate until the relative density is 1.2-1.25, adding ethanol with the volume of 1.5 times of the volume of the filtrate, standing the filtrate for 24 hours, and taking supernatant to obtain alcohol-soluble substance;

and step four, mixing the clear distillate and the alcohol solution, adjusting the pH value to 4.1-5.5, adding sorbic acid, sterilizing at 100 ℃ for 20min, filling to obtain a finished compound origanum oral liquid for treating piglet diarrhea, and refrigerating for later use.

2. The method for preparing the compound origanum vulgare oral liquid for treating piglet diarrhea according to claim 1, wherein the origanum vulgare raw material is obtained from wild origanum vulgare domesticated species of Dabie mountain or Funiu mountain.

3. The preparation method of the compound origanum vulgare oral liquid for treating piglet diarrhea according to claim 1, wherein in the first step, 80 parts by weight of origanum vulgare, 50 parts by weight of coptis chinensis, 50 parts by weight of folium artemisiae argyi, 40 parts by weight of radix sophorae flavescentis and 200 parts by weight of elsholtzia.

4. The preparation method of the compound origanum vulgare oral liquid for treating piglet diarrhea according to claim 1, wherein in the second step, the temperature of the two distillations is 80-100 ℃, and the interval between the two distillations is 15-45 min.

5. The preparation method of the compound origanum vulgare oral liquid for treating piglet diarrhea according to claim 1, wherein in the third step, a 5um filter membrane is adopted for filtration.

6. The method for preparing the compound origanum vulgare oral liquid for treating piglet diarrhea according to claim 1, wherein in the fourth step, the pH is adjusted by a sodium phosphate buffer solution.

7. The use method of the compound oregano oral liquid for treating piglet diarrhea obtained in the claim 1 is characterized in that the compound oregano oral liquid is used for treating piglet diarrhea and comprises the following steps:

the compound origanum oral liquid for treating piglet diarrhea is orally taken by piglets with diarrhea, and is taken once a day with 2-4ml per head.