CN106259159A - A kind of duck culturing method preventing and treating E. coli isolated from ducks - Google Patents
A kind of duck culturing method preventing and treating E. coli isolated from ducks Download PDFInfo
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- CN106259159A CN106259159A CN201610598908.6A CN201610598908A CN106259159A CN 106259159 A CN106259159 A CN 106259159A CN 201610598908 A CN201610598908 A CN 201610598908A CN 106259159 A CN106259159 A CN 106259159A
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Abstract
The invention belongs to poultry farming field, specifically disclose a kind of duck culturing method preventing and treating E. coli isolated from ducks, according to week old, by the diseases prevention duck feed of corresponding amount, duck is fed, and 3 times/day, simultaneously according to certain rule diseases prevention a certain amount of to Corium Anas domestica hemostasis injection；And all duck is put in a suitable place to breed in nuisanceless rice field after feeding every time.Wherein, described diseases prevention duck feed is made up of according to certain part by weight energy feed, trace mineral supplement and Chinese herbal medicine additive；Described Chinese herbal medicine additive and diseases prevention injection include that the raw material components of following weight portion is made up of according to certain part by weight Rhizoma Coptidis, Herba Taraxaci, Flos Lonicerae, Cortex Moutan, Caulis Akebiae, Herba Portulacae, Herba Patriniae, Fructus Trichosanthis, the Radix Paeoniae Alba and Radix Glycyrrhizae.The duck culturing method of the present invention, not only raw material green safety, easy and simple to handle, with low cost, and can avoid that medicine is residual, the problem of drug resistance, effectively prevent and treat E. coli isolated from ducks.
Description
[technical field]
The present invention relates to poultry cultivating technology, be specifically related to a kind of duck culturing method preventing and treating E. coli isolated from ducks.
[background technology]
Duck E．coli is a kind of acute septic infectious disease caused by escherichia coli, so having another name called E. coli isolated from ducks
Septicemia.Escherichia coli are widely distributed in nature, and the duck of various ages in days is the most susceptible, especially the most susceptible with duckling, fall ill up to
70%-90%.When duck field, sanitary condition is poor, and ground is moist, improper ventilation in house, and ammonia odor is big, and stocking density is excessive, often
Morbidity.The most sick fowl of the source of infection of primary disease and the birds carried disease germs, such as their corpse and feces, pollution feedstuff, drinking-water, pond
The pool, raising place, instrument and hatching egg etc. are also the sources of infection, and healthy duck passes through escherichia coli in digestive tract or respiratory tract infection, but
The most at once falling ill, when Abwehrkraft des Koepers reduces or suffers from other diseases, the internal antibacterial that lives away from home just seizes the opportunity intravasation, with
Blood distribution whole body, causes septicemia or other diseases；Also can enter blood by skin wound, cause septicemia；It addition, duck
Hematophagus in house and ectozoa etc., it is possible to cause the generation of primary disease.
Primary disease is many in acute sepsis process, and after newly going out the duckling morbidity of shell, body constitution weakness, necking down of sleeping, abdominal circumference are relatively big,
Often there is dysentery, how because of septicemia death；Listless, loss of appetite after bigger duckling morbidity, two wings are sagging, stand on one side,
Head is bent downwardly, the corners of the mouth contacts to earth and normal attached glutinous secretions at two and nostril；The sick duck having draws green loose stool, dyspnea, often
Because of septicemia or body constitution exhaustion, dehydration death；Sick duck happiness of growing up crouches, be reluctant to walk about, abdominal circumference expands sagging in penguin shape when standing,
Palpation abdominal part has liquid wave dynamic, punctures stream ascites.
At present, generally by inoculation multivalence escherichia coli inactivated vaccine or carry out with duck oromeningitis-escherichia coli hyper-immune serum
Prevention and treatment, but it is the most of a great variety to be because escherichia coli, and be easy to produce drug resistance, actual production is infected way
Footpath and infection reason differ, and therefore, are not only difficult to prevent and cure, and spend height and to produce medicine residual.
[summary of the invention]
The goal of the invention of the present invention is: for the problem of above-mentioned existence, it is provided that a kind of duck culturing preventing and treating E. coli isolated from ducks
Method, its using the Chinese herbal medicine of green safety as raw material, each Chinese herbal medicine synergism, produce heat clearing and damp drying, eliminating fire and detoxication, disappear
Swollen eliminating stagnation and the effect with blood repercussive, such that it is able to conditioning duck is inside, strengthens the resistance against diseases of duck, thus effectively preventing and treating duck be big
Enterobacteria is sick；It addition, this method is simple to operate, low cost and easy to implement.
To achieve these goals, the technical solution used in the present invention is as follows:
A kind of duck culturing method preventing and treating E. coli isolated from ducks, for the duck within 2 week old, feeds by diseases prevention duck feed, 3
Times/day, and 0.1-0.3g every time, simultaneously to Corium Anas domestica hemostasis diseases prevention injection, 2 days/time, and 0.8mL every time；To 3-5 week old
Within duck, feed by diseases prevention duck feed, 3 times/day, and every time 0.3-0.6g, Corium Anas domestica hemostasis diseases prevention injected simultaneously
Liquid, 1 day/time, and 1.0mL every time；For ducks more than 5 week old, feed by diseases prevention duck feed, 3 times/day, allow it every time
Free choice feeding is to all you can eat, simultaneously to Corium Anas domestica hemostasis diseases prevention injection, and 4 days/time, and 1.0mL every time；And after feeding every time
All duck is put in a suitable place to breed in nuisanceless rice field；
Described diseases prevention duck feed is by energy feed 300-400 part, trace mineral supplement 1-3 part and Chinese herbal medicine additive 5-
18 parts of compositions；
Described Chinese herbal medicine additive and diseases prevention injection include the raw material components of following weight portion: Rhizoma Coptidis 8-21 part, Pu Gong
English 8-21 part, Flos Lonicerae 7-19 part, Cortex Moutan 7-18 part, Caulis Akebiae 5-16 part, Herba Portulacae 5-16 part, Herba Patriniae 4-16 part, Fructus Trichosanthis 3-
14 parts, Radix Paeoniae Alba 3-12 part and Radix Glycyrrhizae 2-10 part.
In the present invention, further, described Chinese herbal medicine additive and diseases prevention injection include the raw material of following weight portion
Component: Rhizoma Coptidis 13-18 part, Herba Taraxaci 13-18 part, Flos Lonicerae 12-16 part, Cortex Moutan 11-15 part, Caulis Akebiae 9-14 part, Herba Portulacae 9-
13 parts, Herba Patriniae 8-13 part, Fructus Trichosanthis 7-11 part, Radix Paeoniae Alba 7-11 part and Radix Glycyrrhizae 3-7 part.
In the present invention, further, described Chinese herbal medicine additive and diseases prevention injection include the raw material of following weight portion
Component: Rhizoma Coptidis 16 parts, Herba Taraxaci 16 parts, Flos Lonicerae 14 parts, Cortex Moutan 13 parts, Caulis Akebiae 11 parts, Herba Portulacae 10 parts, Herba Patriniae 10 parts,
Fructus Trichosanthis 9 parts, the Radix Paeoniae Alba 8 parts and 5 parts of Radix Glycyrrhizae.
In the present invention, further, described energy feed is by Semen Maydis powder 50-65 part, Semen phaseoli radiati powder 20-30 part and rapeseed meal
After 18-28 part mix homogeneously, make through coarse pulverization 80 mesh that sieve；Described trace mineral supplement by conch meal 2-4 part and
Pollen Pini 1-3 part mixes to uniformly making.
In the present invention, further, described diseases prevention duck feed is made up of step in detail below:
(1) by each herbal component Rhizoma Coptidis, Herba Taraxaci, Flos Lonicerae, Cortex Moutan, Caulis Akebiae, Herba Portulacae, Herba Patriniae, Fructus Trichosanthis, the Radix Paeoniae Alba
Clean respectively with Radix Glycyrrhizae, weigh each herbal component the most by weight, standby；
(2) putting in beaker by each herbal component weighed in (1) mixing, adding volumetric concentration in beaker is
The ethanol of 85-90%, to each herbal component of just submergence, is heated to reflux, and circulation is filtered after extracting 3 times, and filtrate is placed in vacuum
Under the conditions of, through concentrating and after atomization drying, coarse pulverization also crosses 100 mesh sieves, i.e. obtains Chinese medicine powder, standby；
(3) by weight, (2) gained Chinese medicine powder is stirred to mixing homogeneously with trace mineral supplement, energy feed,
After superfine comminution at low temperature 200 mesh sieves excessively, put into granulator and make granule, after packaging, i.e. can get finished product.
In the present invention, further, in step (3), a diameter of 2.5-3.8mm of described granule.
In the present invention, further, described diseases prevention injection is made up of step in detail below:
(1) by each herbal component Rhizoma Coptidis, Herba Taraxaci, Flos Lonicerae, Cortex Moutan, Caulis Akebiae, Herba Portulacae, Herba Patriniae, Fructus Trichosanthis, the Radix Paeoniae Alba
Clean respectively with Radix Glycyrrhizae, weigh each herbal component the most by weight, standby；
(2) putting in beaker by each herbal component weighed in (1) mixing, adding volumetric concentration in beaker is
The ethanol of 85-90%, to each herbal component of just submergence, is heated to reflux, and circulation is filtered after extracting 3 times, filtrate is put into centrifugal
With the centrifugation 30min of 2000r/min in machine, obtain herb liquid, standby；
(3) herb liquid in (2) is placed under vacuum condition, is concentrated into the thick paste that water content is 15-21%, by gained
Thick paste is put to room temperature, and adding volume is the glycerol of 10-15%, and adds the physiology salt with thick paste and glycerol same volume
Water, stirs to mix homogeneously, filters, obtain filtrate after 2 DEG C of cold preservations stand 40 hours；Gained filtrate the most successively through 0.45 μm,
The microporous filter membrane circulating filtration of 0.22 μm 2 times, i.e. obtains the diseases prevention injection of 1g of the present invention crude drug/mL after embedding, sterilizing.
In sum, owing to have employed technique scheme, the invention has the beneficial effects as follows:
(1) the duck culturing method of the present invention, by according to certain rule, feeding a certain amount of diseases prevention duck feed or note to duck
Penetrate certain diseases prevention injection, duplicate protection, strengthen prevention effect, and method is easy and simple to handle, with low cost, easy to implement, suitable
Plant for various scales.
(2) present invention with the addition of the trace mineral supplement formed with conch meal and Pollen Pini, is possible not only to effectively mend
Fill trace element necessary to duck and aminoacid, it is also possible to increase the fragrance of feedstuff, strengthen digestion power, improve searching for food of duck
Rate, thus improve Daily gain；And using natural Chinese herbal medicine as additive and the raw material of injection, not only low cost, without medicine
Residual and do not produce drug resistance, and the effect for the treatment of both the principal and secondary aspects of a disease can be reached, can effectively prevent and treat E. coli isolated from ducks.Wherein, with Huang
Company, Herba Taraxaci, Flos Lonicerae and Cortex Moutan are main, are auxiliary with Caulis Akebiae, Herba Portulacae and Herba Patriniae, and Fructus Trichosanthis and the Radix Paeoniae Alba are assistant, Radix Glycyrrhizae for making,
Coordinating the actions of various ingredients in a prescription, and the sweet taste of Herba Taraxaci, Flos Lonicerae, Fructus Trichosanthis and Radix Glycyrrhizae and Rhizoma Coptidis, Cortex Moutan, Caulis Akebiae, Herba Portulacae, Herba Patriniae and the Radix Paeoniae Alba
Picric acid taste coordinate mutually to merge, and the most cooler property of each medical herbs, common effect reaches the effect that taste levelling is cool, collaborative plays heat clearing away
Dampness, eliminating fire and detoxication, dispersing swelling and dissipating binds and and the effect of blood repercussive, such that it is able to conditioning duck inside, strengthen duck resistance against diseases,
Thus effectively prevent and treat duck E．coli.Pharmacology and the property of medicine of each Chinese herbal medicine are as follows:
Rhizoma Coptidis: bitter in the mouth, cold in nature, has effect of heat clearing and damp drying, eliminating fire and detoxication and parasite killing, gulps down for damp and hot feeling of fullness, vomiting
The diseases such as acid, dysentery, jaundice, unconsciousness due to high fever, hyperactivity of heart-fire and dysphoria and insomnia.
Herba Taraxaci: sweet in the mouth, micro-hardship, cold in nature；There is effect of heat-clearing and toxic substances removing, dispersing swelling and dissipating binds, can control upper respiratory tract infection,
The diseases such as acute mastitis swells and ache, dysentery, gallbladder assist inflammation, urinary system infection, carbuncle furuncle furuncle, scrofula, furuncle carbuncle toxin.
Flos Lonicerae: sweet in the mouth is cold in nature, has dispelling wind-heat, effect of removing summer-heat blood poison, for various febrile diseases, such as fever of the body, sends out
The diseases such as rash, speckle, pyretic toxicity carbuncle sore, laryngopharynx swelling and pain.
Cortex Moutan: bitter in the mouth, pungent, cold nature, there is clearing away heat and cooling blood and effect of blood repercussive, the pathogenic heat attacking blood system in febrile diseases can be controlled, send out speckle, frightened
Epilepsy, tells, is defeated in battle, has blood in stool, the disease such as abdominal mass, carbuncle and ulcer and traumatic injury.
Caulis Akebiae: bitter in the mouth, cool in nature, has pathogenic fire purging row water, effect of promoting blood circulation, can control hot urination, stranguria with turbid discharge, edema, breast
The diseases such as middle dysphoria with smothery sensation, sore throat pharyngalgia, inflexible pain all over.
Herba Portulacae: sour in the mouth is cold in nature, have clearing away heat-damp and promoting diuresis, removing toxic substances and promoting subsidence of swelling, antiinflammatory, quench the thirst, diuresis, pyretic toxicity blood can be controlled
Dysentery, carbuncle furuncle, eczema, snake bite and insect sting, have blood in stool and the disease such as metrostaxis.
Herba Patriniae: acrid in the mouth, hardship, cool in nature, there is the effect such as heat-clearing and toxic substances removing, removing blood stasis and expelling pus, for appendicitis, dysentery, enteritis,
The diseases such as hepatitis, eye conjunctivitis, postnatal blood stasis stomachache.
Fructus Trichosanthis: sweet in the mouth, micro-hardship, cold in nature, have antipyretic quench the thirst, diuresis, the effect of relieving cough and expelling phlegm, for cough due to lung-heat, expectorant
Turbid Huang is thick, obstruction of qi in the chest and cardialgia, blocked-up chest feeling of fullness, acute appendicitis swell and ache and the disease such as constipation.
The Radix Paeoniae Alba: bitter in the mouth, acid, cold nature, has effect of suppressing the hyperactive liver pain relieving, nourishing blood for regulating menstruation and astringing YIN to stop sweating, is used for having a headache dizzy
Dizzy, stomachache, limb pain twin, the disease such as blood deficiency and yellow complexion, menoxenia and night sweat.
Radix Glycyrrhizae: sweet in the mouth is put down, have relieve inflammation or internal heat, heat-clearing and toxic substances removing, nourishing the lung to arrest cough and effect of invigorating the spleen and replenishing QI, cure mainly swelling throat
Bitterly, cellulitis skin infection, gastrointestinal ulceration and solution poison of drug, alimentary toxicosis etc..
[detailed description of the invention]
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment 1
The most by weight, following raw materials is weighed:
Energy feed: 300 parts；Chinese herbal medicine additive: 5 parts.
Wherein, energy feed includes the raw material of following weight portion:
Semen Maydis powder: 50 parts；Semen phaseoli radiati powder: 20 parts；
Rapeseed meal: 18 parts.
Trace mineral supplement is made up of the raw material of following weight portion:
Conch meal: 2 parts；Pollen Pini: 1 part.
Chinese herbal medicine additive includes the raw material components of following weight portion:
2. produce diseases prevention duck feed according to step in detail below:
(1) by each herbal component Rhizoma Coptidis, Herba Taraxaci, Flos Lonicerae, Cortex Moutan, Caulis Akebiae, Herba Portulacae, Herba Patriniae, Fructus Trichosanthis, the Radix Paeoniae Alba
Clean respectively with Radix Glycyrrhizae, weigh each herbal component the most by weight, standby；
(2) putting in beaker by each herbal component weighed in (1) mixing, adding volumetric concentration in beaker is
The ethanol of 85%, to each herbal component of just submergence, is heated to reflux, and circulation is filtered after extracting 3 times, and filtrate is placed in vacuum bar
Under part, through concentrating and after atomization drying, coarse pulverization also crosses 100 mesh sieves, i.e. obtains Chinese medicine powder, standby；
(3) by weight, (2) gained Chinese medicine powder is stirred to mixing homogeneously with trace mineral supplement, energy feed,
After superfine comminution at low temperature 200 mesh sieves excessively, put into granulator and make the granule of a diameter of 2.5mm, the most available after packaging
Finished product.
3. produce diseases prevention injection according to step in detail below:
(1) by each herbal component Rhizoma Coptidis, Herba Taraxaci, Flos Lonicerae, Cortex Moutan, Caulis Akebiae, Herba Portulacae, Herba Patriniae, Fructus Trichosanthis, the Radix Paeoniae Alba
Clean respectively with Radix Glycyrrhizae, weigh each herbal component the most by weight, standby；
(2) putting in beaker by each herbal component weighed in (1) mixing, adding volumetric concentration in beaker is
The ethanol of 85%, to each herbal component of just submergence, is heated to reflux, and circulation is filtered after extracting 3 times, and filtrate is put into centrifuge
In with the centrifugation 30min of 2000r/min, obtain herb liquid, standby；
(3) herb liquid in (2) is placed under vacuum condition, is concentrated into the thick paste that water content is 15%, thick by gained
Cream is put to room temperature, and adding volume is the glycerol of 10%, and adds and thick paste and the normal saline of glycerol same volume, stirring
To mix homogeneously, filter after 2 DEG C of cold preservations stand 40 hours, obtain filtrate；Gained filtrate the most successively through 0.45 μm, 0.22 μm micro-
Hole filter membrane circulating filtration 2 times, i.e. obtains the diseases prevention injection of 1g of the present invention crude drug/mL after embedding, sterilizing.
4. according to method in detail below, duck is fed:
For the duck within 2 week old, feed by diseases prevention duck feed, 3 times/day, and 0.1g every time, simultaneously under Corium Anas domestica
Injection diseases prevention injection, 2 days/time, and 0.8mL every time；To the duck within 3-5 week old, feed by diseases prevention duck feed, 3 times/
My god, and 0.3g every time, simultaneously to Corium Anas domestica hemostasis diseases prevention injection, 1 day/time, and 1.0mL every time；More than 5 week old
Duck, feeds by diseases prevention duck feed, 3 times/day, allows its free choice feeding to all you can eat every time, prevents Corium Anas domestica hemostasis simultaneously
Sick injection, 4 days/time, and 1.0mL every time；And all duck is put in a suitable place to breed in nuisanceless rice field after feeding every time.
Embodiment 2
The most by weight, following raw materials is weighed:
Energy feed: 350 parts；Chinese herbal medicine additive: 11 parts.
Wherein, energy feed includes the raw material of following weight portion:
Semen Maydis powder: 57 parts；Semen phaseoli radiati powder: 25 parts；
Rapeseed meal: 22 parts.
Trace mineral supplement is made up of the raw material of following weight portion:
Conch meal: 3 parts；Pollen Pini: 2 parts.
Chinese herbal medicine additive includes the raw material components of following weight portion:
2. produce diseases prevention duck feed according to step in detail below:
(1) by each herbal component Rhizoma Coptidis, Herba Taraxaci, Flos Lonicerae, Cortex Moutan, Caulis Akebiae, Herba Portulacae, Herba Patriniae, Fructus Trichosanthis, the Radix Paeoniae Alba
Clean respectively with Radix Glycyrrhizae, weigh each herbal component the most by weight, standby；
(2) putting in beaker by each herbal component weighed in (1) mixing, adding volumetric concentration in beaker is
The ethanol of 88%, to each herbal component of just submergence, is heated to reflux, and circulation is filtered after extracting 3 times, and filtrate is placed in vacuum bar
Under part, through concentrating and after atomization drying, coarse pulverization also crosses 100 mesh sieves, i.e. obtains Chinese medicine powder, standby；
(3) by weight, (2) gained Chinese medicine powder is stirred to mixing homogeneously with trace mineral supplement, energy feed,
After superfine comminution at low temperature 200 mesh sieves excessively, put into granulator and make the granule of a diameter of 3.0mm, the most available after packaging
Finished product.
3. produce diseases prevention injection according to step in detail below:
(1) by each herbal component Rhizoma Coptidis, Herba Taraxaci, Flos Lonicerae, Cortex Moutan, Caulis Akebiae, Herba Portulacae, Herba Patriniae, Fructus Trichosanthis, the Radix Paeoniae Alba
Clean respectively with Radix Glycyrrhizae, weigh each herbal component the most by weight, standby；
(2) putting in beaker by each herbal component weighed in (1) mixing, adding volumetric concentration in beaker is
The ethanol of 88%, to each herbal component of just submergence, is heated to reflux, and circulation is filtered after extracting 3 times, and filtrate is put into centrifuge
In with the centrifugation 30min of 2000r/min, obtain herb liquid, standby；
(3) herb liquid in (2) is placed under vacuum condition, is concentrated into the thick paste that water content is 19%, thick by gained
Cream is put to room temperature, and adding volume is the glycerol of 13%, and adds and thick paste and the normal saline of glycerol same volume, stirring
To mix homogeneously, filter after 2 DEG C of cold preservations stand 40 hours, obtain filtrate；Gained filtrate the most successively through 0.45 μm, 0.22 μm micro-
Hole filter membrane circulating filtration 2 times, i.e. obtains the diseases prevention injection of 1g of the present invention crude drug/mL after embedding, sterilizing.
4. according to method in detail below, duck is fed:
For the duck within 2 week old, feed by diseases prevention duck feed, 3 times/day, and 0.2g every time, simultaneously under Corium Anas domestica
Injection diseases prevention injection, 2 days/time, and 0.8mL every time；To the duck within 3-5 week old, feed by diseases prevention duck feed, 3 times/
My god, and 0.4g every time, simultaneously to Corium Anas domestica hemostasis diseases prevention injection, 1 day/time, and 1.0mL every time；More than 5 week old
Duck, feeds by diseases prevention duck feed, 3 times/day, allows its free choice feeding to all you can eat every time, prevents Corium Anas domestica hemostasis simultaneously
Sick injection, 4 days/time, and 1.0mL every time；And all duck is put in a suitable place to breed in nuisanceless rice field after feeding every time.
Embodiment 3
The most by weight, following raw materials is weighed:
Energy feed: 400 parts；Chinese herbal medicine additive: 18 parts.
Wherein, energy feed includes the raw material of following weight portion:
Semen Maydis powder: 65 parts；Semen phaseoli radiati powder: 30 parts；
Rapeseed meal: 28 parts.
Trace mineral supplement is made up of the raw material of following weight portion:
Conch meal: 4 parts；Pollen Pini: 3 parts.
Chinese herbal medicine additive includes the raw material components of following weight portion:
2. produce diseases prevention duck feed according to step in detail below:
(1) by each herbal component Rhizoma Coptidis, Herba Taraxaci, Flos Lonicerae, Cortex Moutan, Caulis Akebiae, Herba Portulacae, Herba Patriniae, Fructus Trichosanthis, the Radix Paeoniae Alba
Clean respectively with Radix Glycyrrhizae, weigh each herbal component the most by weight, standby；
(2) putting in beaker by each herbal component weighed in (1) mixing, adding volumetric concentration in beaker is
The ethanol of 90%, to each herbal component of just submergence, is heated to reflux, and circulation is filtered after extracting 3 times, and filtrate is placed in vacuum bar
Under part, through concentrating and after atomization drying, coarse pulverization also crosses 100 mesh sieves, i.e. obtains Chinese medicine powder, standby；
(3) by weight, (2) gained Chinese medicine powder is stirred to mixing homogeneously with trace mineral supplement, energy feed,
After superfine comminution at low temperature 200 mesh sieves excessively, put into granulator and make the granule of a diameter of 3.8mm, the most available after packaging
Finished product.
3. produce diseases prevention injection according to step in detail below:
(1) by each herbal component Rhizoma Coptidis, Herba Taraxaci, Flos Lonicerae, Cortex Moutan, Caulis Akebiae, Herba Portulacae, Herba Patriniae, Fructus Trichosanthis, the Radix Paeoniae Alba
Clean respectively with Radix Glycyrrhizae, weigh each herbal component the most by weight, standby；
(2) putting in beaker by each herbal component weighed in (1) mixing, adding volumetric concentration in beaker is
The ethanol of 90%, to each herbal component of just submergence, is heated to reflux, and circulation is filtered after extracting 3 times, and filtrate is put into centrifuge
In with the centrifugation 30min of 2000r/min, obtain herb liquid, standby；
(3) herb liquid in (2) is placed under vacuum condition, is concentrated into the thick paste that water content is 21%, thick by gained
Cream is put to room temperature, and adding volume is the glycerol of 15%, and adds and thick paste and the normal saline of glycerol same volume, stirring
To mix homogeneously, filter after 2 DEG C of cold preservations stand 40 hours, obtain filtrate；Gained filtrate the most successively through 0.45 μm, 0.22 μm micro-
Hole filter membrane circulating filtration 2 times, i.e. obtains the diseases prevention injection of 1g of the present invention crude drug/mL after embedding, sterilizing.
4. according to method in detail below, duck is fed:
For the duck within 2 week old, feed by diseases prevention duck feed, 3 times/day, and 0.3g every time, simultaneously under Corium Anas domestica
Injection diseases prevention injection, 2 days/time, and 0.8mL every time；To the duck within 3-5 week old, feed by diseases prevention duck feed, 3 times/
My god, and 0.6g every time, simultaneously to Corium Anas domestica hemostasis diseases prevention injection, 1 day/time, and 1.0mL every time；More than 5 week old
Duck, feeds by diseases prevention duck feed, 3 times/day, allows its free choice feeding to all you can eat every time, prevents Corium Anas domestica hemostasis simultaneously
Sick injection, 4 days/time, and 1.0mL every time, and all duck is put in a suitable place to breed in nuisanceless rice field after feeding every time.
Compliance test result: select 100 ducklings from certain duckery, does not has obvious ecocline between these 100 ducklings, will
They are equally divided into two groups.Raise according to a conventional method, as a control group for one group；Another group is raised according to the method for the present invention
Support, as experimental group.Through feeding to delivering for sale, contrast sickness rate and the mortality rate thereof of its E. coli isolated from ducks, the results are shown in Table 1:
Table 1
| Result is added up | Sickness rate | Mortality rate |
| Matched group | 65-85% | 35-55% |
| Experimental group | 6-9% | 3-5% |
As seen from the above table, contrast, the sickness rate of the E. coli isolated from ducks of the duck culturing method of the present invention are thought with conventional duck culturing method
The lowest with mortality rate 59-76% and 33-50%, therefore, the method for the present invention can effectively prevent and treat E. coli isolated from ducks, permissible
Effectively reduce the loss of cultivation, increase economic efficiency.
Described above is the detailed description for the preferable possible embodiments of the present invention, but embodiment is not limited to this
Bright patent claim, the equal change completed under the technical spirit suggested by all present invention or modification change, all should belong to
In the contained the scope of the claims of the present invention.
Claims (7)
1. the duck culturing method preventing and treating E. coli isolated from ducks, it is characterised in that: for the duck within 2 week old, use diseases prevention duck feed
Feed, 3 times/day, and 0.1-0.3g every time, simultaneously to Corium Anas domestica hemostasis diseases prevention injection, 2 days/time, and 0.8mL every time；
To the duck within 3-5 week old, feed by diseases prevention duck feed, 3 times/day, and 0.3-0.6g every time, simultaneously to Corium Anas domestica hemostasis
Diseases prevention injection, 1 day/time, and 1.0mL every time；For ducks more than 5 week old, feed by diseases prevention duck feed, 3 times/day,
Allow every time its free choice feeding to all you can eat, simultaneously to Corium Anas domestica hemostasis diseases prevention injection, 4 days/time, and every time 1.0mL；And it is every
Secondary feed after all duck is put in a suitable place to breed in nuisanceless rice field；
Described diseases prevention duck feed is by energy feed 300-400 part, trace mineral supplement 1-3 part and Chinese herbal medicine additive 5-18 part
Composition；
Described Chinese herbal medicine additive and diseases prevention injection include the raw material components of following weight portion: Rhizoma Coptidis 8-21 part, Herba Taraxaci 8-
21 parts, Flos Lonicerae 7-19 part, Cortex Moutan 7-18 part, Caulis Akebiae 5-16 part, Herba Portulacae 5-16 part, Herba Patriniae 4-16 part, Fructus Trichosanthis 3-14 part,
Radix Paeoniae Alba 3-12 part and Radix Glycyrrhizae 2-10 part.
A kind of duck culturing method preventing and treating E. coli isolated from ducks, it is characterised in that described Chinese herbal medicine adds
Agent and diseases prevention injection include the raw material components of following weight portion: Rhizoma Coptidis 13-18 part, Herba Taraxaci 13-18 part, Flos Lonicerae 12-16
Part, Cortex Moutan 11-15 part, Caulis Akebiae 9-14 part, Herba Portulacae 9-13 part, Herba Patriniae 8-13 part, Fructus Trichosanthis 7-11 part, Radix Paeoniae Alba 7-11 part and sweet
Grass 3-7 part.
A kind of duck culturing method preventing and treating E. coli isolated from ducks, it is characterised in that described Chinese herbal medicine adds
Agent and diseases prevention injection include the raw material components of following weight portion: Rhizoma Coptidis 16 parts, Herba Taraxaci 16 parts, Flos Lonicerae 14 parts, Cortex Moutan 13
Part, Caulis Akebiae 11 parts, Herba Portulacae 10 parts, Herba Patriniae 10 parts, Fructus Trichosanthis 9 parts, the Radix Paeoniae Alba 8 parts and 5 parts of Radix Glycyrrhizae.
A kind of duck culturing method preventing and treating E. coli isolated from ducks, it is characterised in that described energy feed by
After Semen Maydis powder 50-65 part, Semen phaseoli radiati powder 20-30 part and rapeseed meal 18-28 part mix homogeneously, make through coarse pulverization 80 mesh that sieve；
Described trace mineral supplement is mixed to uniformly making by conch meal 2-4 part and Pollen Pini 1-3 part.
5. according to the duck culturing method preventing and treating E. coli isolated from ducks a kind of described in claim 1-4 any one, it is characterised in that described
Diseases prevention duck feed is made up of step in detail below:
(1) by each herbal component Rhizoma Coptidis, Herba Taraxaci, Flos Lonicerae, Cortex Moutan, Caulis Akebiae, Herba Portulacae, Herba Patriniae, Fructus Trichosanthis, the Radix Paeoniae Alba and sweet
Grass cleans respectively, weighs each herbal component the most by weight, standby；
(2) putting in beaker by each herbal component weighed in (1) mixing, adding volumetric concentration in beaker is 85-
The ethanol of 90%, to each herbal component of just submergence, is heated to reflux, and circulation is filtered after extracting 3 times, and filtrate is placed in vacuum bar
Under part, through concentrating and after atomization drying, coarse pulverization also crosses 100 mesh sieves, i.e. obtains Chinese medicine powder, standby；
(3) by weight, (2) gained Chinese medicine powder is stirred to mixing homogeneously with trace mineral supplement, energy feed, passes through
After superfine comminution at low temperature excessively 200 mesh sieves, put into granulator and make granule, after packaging, i.e. can get finished product.
A kind of duck culturing method preventing and treating E. coli isolated from ducks, it is characterised in that in step (3), described
A diameter of 2.5-3.8mm of granule.
7. according to the duck culturing method preventing and treating E. coli isolated from ducks a kind of described in claim 1-4 any one, it is characterised in that described
Diseases prevention injection is made up of step in detail below:
(1) by each herbal component Rhizoma Coptidis, Herba Taraxaci, Flos Lonicerae, Cortex Moutan, Caulis Akebiae, Herba Portulacae, Herba Patriniae, Fructus Trichosanthis, the Radix Paeoniae Alba and sweet
Grass cleans respectively, weighs each herbal component the most by weight, standby；
(2) putting in beaker by each herbal component weighed in (1) mixing, adding volumetric concentration in beaker is 85-
The ethanol of 90%, to each herbal component of just submergence, is heated to reflux, and circulation is filtered after extracting 3 times, and filtrate is put into centrifuge
In with the centrifugation 30min of 2000r/min, obtain herb liquid, standby；
(3) herb liquid in (2) is placed under vacuum condition, is concentrated into the thick paste that water content is 15-21%, by the thick paste of gained
Putting to room temperature, adding volume is the glycerol of 10-15%, and adds and thick paste and the normal saline of glycerol same volume, stirs
Mix to mix homogeneously, filter after 2 DEG C of cold preservations stand 40 hours, obtain filtrate；Gained filtrate is the most successively through 0.45 μm, 0.22 μm
Microporous filter membrane circulating filtration 2 times, i.e. obtains the diseases prevention injection of 1g of the present invention crude drug/mL after embedding, sterilizing.
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| CN107125068A (en) * | 2017-05-12 | 2017-09-05 | 钟维莉 | A kind of growing rice with ducks method |
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