CN111227257A - Industrial method for refining spirulina protein peptide and spray drying microencapsulation - Google Patents
Industrial method for refining spirulina protein peptide and spray drying microencapsulation Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/66—Proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
- A23L29/206—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
- A23L29/25—Exudates, e.g. gum arabic, gum acacia, gum karaya or tragacanth
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
- A23L29/206—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
- A23L29/256—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin from seaweeds, e.g. alginates, agar or carrageenan
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
- A23L29/35—Degradation products of starch, e.g. hydrolysates, dextrins; Enzymatically modified starches
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention relates to the technical field of spirulina protein peptide, in particular to an industrial method for refining spirulina protein peptide and spray drying microencapsulation, which can obtain spirulina protein peptide powder with good absorption and dissolution, no fishy, bitter and salty tastes, expands the application range of spirulina in medicine and health food, and can be used in beverages and other beverages; the method comprises the following steps: s1, preprocessing a ceramic filter element; s2, ultrafiltration; s3, nanofiltration; s4, preparing a spirulina protein peptide spray drying feed liquid; s5, pretreatment of the feed liquid; s6, spray drying and embedding; s7, secondary drying; s8, vacuumizing, filling nitrogen and packaging; the preparation method comprises the steps of obtaining small-molecule spirulina protein peptide through ultrafiltration, removing univalent cations such as sodium through nanofiltration membrane filtration, obtaining the spirulina protein peptide microcapsule embedded powder through a spray drying method, removing fishy smell, bitter taste and salty taste, obtaining the spirulina protein peptide microcapsule which contains various active peptides, and simultaneously having the characteristics of good absorption and dissolution, no fishy smell, bitter taste and salty taste.
Description
Technical Field
The invention relates to the technical field of spirulina protein peptide, in particular to an industrial method for refining spirulina protein peptide and spray drying microencapsulation.
Background
Spirulina (Spirulina) belongs to the phylum of Cyanophyta, the family Oscillatoriaceae, and is a kind of spiral prokaryotic algae composed of single cells or multiple cells, and has 35 million years of ancient biology on the earth. The spirulina contains 60-70% of protein, and is rich in VA, VB and minerals. Recommended by the Food and Agriculture Organization (FAO) of the United nations: "21 st century optimal food"; determined by the World Health Organization (WHO): "best health products for human 21 st century"; the second international conference on microbial proteins identified in 1972: "future super nutritious food"; in 1974, the method is accepted by food conference in the united nations: super nutrient food. In the early 70 s, the Ministry of agriculture and the national Commission of science in China put spirulina into the items of 'seven five', 'eight five' attack and popularization. Because of its high safety, spirulina is eaten without adverse effect so far, and can be popularized and eaten in large quantities for a long time without any toxic and side effect in human body, and spirulina is listed as common food management by the ministry of health of China in 8 months 2004 (No. 17 bulletin of the ministry of health 2004).
A large number of researches show that the spirulina has the effects of scavenging free radicals, resisting oxidation and resisting inflammation; supplementing high-quality protein, vitamins and minerals, promoting differentiation and proliferation of hematopoietic progenitor cells, and promoting protein synthesis; enhancing immunity, and promoting immune system function; has anti-tumor effect, and can inhibit growth of tumor cells and induce apoptosis of tumor cells; nerve tissue and DNA can be protected from being damaged; can improve the radiation resistance of organisms and the like, and is widely applied to the development of health-care food and medicines. However, the dry powder has poor solubility, strong fishy smell and poor protein stability, so that the further development of the spirulina in the fields of food health care and medicine is limited. The small molecular peptide obtained by hydrolyzing with enzyme not only greatly improves the solubility, but also more importantly obtains the functional peptides such as antioxidant active peptide, immunoregulatory active peptide, antitumor active peptide, antihypertensive active peptide, radioresistant active peptide and the like, and simultaneously, the small molecular active peptide can be directly and quickly absorbed, thereby improving the digestion and absorption utilization rate. However, a large amount of hydrophobic amino acids are exposed due to enzymolysis, so that the bitter taste is greatly increased, and the salty taste is greatly increased due to the enrichment of minerals.
Disclosure of Invention
In order to solve the technical problems, the invention provides a spirulina protein peptide powder which has good absorption and dissolution, no fishy, bitter and salty tastes, expands the application range of spirulina in medicines and health-care foods, and can be used for refining spirulina protein peptide in beverages and other beverages and an industrial method for spray drying and microencapsulation.
The invention relates to an industrial method for refining spirulina protein peptide and spray drying microencapsulation, which comprises the following steps:
s1, pretreatment of the ceramic filter element: adding a spirulina protein peptide solution obtained by carrying out enzymolysis and decoloration on spirulina into a stainless steel storage tank, and carrying out filtration pretreatment by using tubular ceramic microfiltration equipment;
s2, ultrafiltration: carrying out ultrafiltration on the pretreated feed liquid in the step S1 by ultrafiltration equipment to obtain micromolecule spirulina protein peptide;
s3, nanofiltration: carrying out three-stage nanofiltration on the micromolecule spirulina protein peptide obtained in the S2 by using ultrafiltration equipment;
s4, preparing spirulina protein peptide spray drying feed liquid: preparing the spirulina protein peptide obtained from the S3 into spirulina protein peptide spray drying feed liquid according to the following formula: 20-30% of spirulina protein peptide; 2-4% of maltodextrin; 3-5% of algin; 3-5% of Arabic gum;
s5, pretreatment of the feed liquid: pasteurizing the prepared spirulina protein peptide spray-dried feed liquid, and homogenizing in a homogenizer;
s6, spray drying and embedding: spray drying the pretreated spirulina protein peptide spray-dried feed liquid by using spray drying equipment, wherein the air inlet temperature is 135-175 ℃, and the air outlet temperature is 65-85 ℃;
s7, secondary drying: connecting a fluidized bed at a powder outlet of the spray drying equipment for secondary drying;
s8, vacuumizing and filling nitrogen for packaging: and packaging the powder obtained after secondary drying by using a vacuumizing nitrogen-filling packaging machine.
Furthermore, the aperture of the ceramic membrane filtering filter element used by the ceramic microfiltration equipment in the S1 is 0.1-0.2 μm.
Furthermore, the aperture of a membrane filtration filter element used by the ultrafiltration equipment in the S2 is 3000-5000 Da.
Further, the aperture of a filtering filter element of a membrane used by the ultra-nanofiltration device in the S3 is 150-300Da, and continuous three-stage nanofiltration is performed on the small-molecule spirulina protein peptide, wherein the primary nanofiltration pressure is 0.35MPa, the secondary nanofiltration pressure is 0.4MPa, and the tertiary nanofiltration pressure is 0.5 MPa.
Furthermore, maltodextrin, algin and acacia in the formula of the feed liquid in the S4 are all natural components.
Further, in the step S5, the pasteurization temperature of the feed liquid is 75-80 ℃, and the time is 40-60 min.
Further, the homogenization pressure in the homogenization treatment in S5 is 25 to 40MPa, and the number of homogenization times is 2 to 4.
Further, the spray drying equipment used in the step S6 is a fluidized bed-pressure spray dryer, the spray pressure is 40-60 MPa, the evaporation capacity is 200-500L/h, and the number of the spray guns used is 1-3.
Furthermore, the air inlet temperature of the fluidized bed in the S7 is 55-77 ℃, and the air outlet temperature is 30-40 ℃.
Further, the vacuum-pumping nitrogen-filled package in the S8 is packaged in an aluminum foil bag with the specification of 1-10 kg, wherein the purity of the filled nitrogen is 99.7-99.9%.
Compared with the prior art, the invention has the beneficial effects that: according to the invention, macromolecular substances such as macromolecular protein, activated carbon, pigment molecules and the like are effectively intercepted by the ceramic membrane microfiltration, so that the ultrafiltration efficiency is improved, and meanwhile, the ultrafiltration membrane can be protected and the service life of the ultrafiltration membrane is prolonged; the invention carries out ultrafiltration by an ultrafiltration membrane, so that the micromolecule peptide below 1000Da reaches more than 90 percent, and the content of micromolecule is greatly improved; according to the invention, monovalent cations such as sodium and the like are removed by continuous three-stage nanofiltration through nanofiltration membrane filtration, the trapped liquid after the first-stage nanofiltration is the feed liquid of the second-stage nanofiltration, and the trapped liquid of the second-stage nanofiltration is the feed liquid of the third-stage nanofiltration, so that the efficiency is greatly improved by the three-stage nanofiltration, the conditions of activity reduction, denaturation, deterioration and the like caused by long-term treatment of the spirulina protein peptide are avoided, and meanwhile, the spirulina protein peptide can be efficiently concentrated, so that the content of the spirulina protein peptide reaches more than 25-30%, the activity of the spirulina protein peptide is well protected by the nanofiltration, the energy utilization is greatly reduced, and the cost is only one tenth of the falling film; drying is carried out by fluidized bed-spray drying, the temperature of the spirulina protein peptide feed liquid in the evaporation process is low, the activity of the protein peptide can be well protected, and meanwhile, secondary drying is carried out by the fluidized bed, so that the spray drying efficiency is greatly improved, and the content of spirulina protein peptide powder is less than 4%; the spirulina protein peptide is embedded by natural embedding agents such as maltodextrin, algin, Arabic gum and the like, so that the stability can be increased, the active protein of the spirulina can be effectively protected, and the fishy smell, the salty taste and the bitter taste of the spirulina protein peptide can be well covered. Greatly expands the application range of the spirulina and can be widely used in medicines, health-care foods, beverage, drinks and granules.
Detailed Description
The following examples are given to further illustrate the embodiments of the present invention. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Example 1
Pretreatment of a ceramic filter element: adding 600L of spirulina protein peptide solution obtained by performing enzymolysis and decoloration on spirulina into a stainless steel storage tank, and pretreating by using tubular ceramic microfiltration equipment, wherein the aperture of a ceramic membrane is 0.1 micron, and the feeding pressure is 0.05MPa, so as to obtain 570L of permeate;
and (3) ultrafiltration: and (3) ultrafiltering 570L of the permeate obtained after the microfiltration of the ceramic membrane by using an ultrafiltration device, wherein the aperture of an ultrafiltration filter core is 3000Da, the membrane inlet pressure is 0.35MPa, and the membrane outlet pressure is 0.05MPa, so that 530L of the small-molecule spirulina protein peptide solution smaller than 3000Da is obtained. Detecting spirulina protein peptide solution, wherein the small molecular peptide with the molecular weight less than 3000Da is 96.2%;
and (4) nanofiltration: performing three-stage nanofiltration on the spirulina protein peptide solution 530L with the small molecular weight of less than 3000Da, wherein the aperture of an ultrafiltration filter core is 150-;
spray drying and embedding: spray drying with fluidized bed-pressure spray dryer. The spirulina protein peptide spray drying feed liquid formula comprises: 28.6 percent of spirulina protein peptide, 2.5 percent of maltodextrin, 4 percent of algin and 5 percent of Arabic gum, and the material liquid is pasteurized at the temperature of 76 ℃ for 60 minutes; homogenizing the sterilized feed liquid at the process homogenization pressure of 30MPa for 2 times, pasteurizing the feed liquid, homogenizing the feed liquid in a homogenizer at the spraying pressure of 30MPa, using 1 spray gun, and controlling the air inlet temperature at 165 ℃ and the air outlet temperature at 72 ℃;
secondary drying: the powder outlet of the spray drying tower is connected with a fluidized bed for secondary drying, the air inlet temperature of the fluidized bed is 65 ℃, the air outlet temperature of the fluidized bed is 32 ℃, the water content of the spirulina peptide microcapsule powder is 3.56% through detection, the embedding rate is 93.2%, the dissolvability is good, and no fishy, salty and bitter taste exists;
vacuumizing and filling nitrogen for packaging: packaging with a vacuum-pumping nitrogen-filling packaging machine, wherein the packaging specification is 1kg aluminum foil bags, and the purity of the filled nitrogen is 99.9%.
Example 2
Pretreatment of a ceramic filter element: adding 3000L of spirulina protein peptide solution obtained by performing enzymolysis and decoloration on spirulina into a stainless steel storage tank, and pretreating with tubular ceramic microfiltration equipment, wherein the pore diameter of a ceramic membrane is 0.1 micron, and the feeding pressure is 0.05MPa, so as to obtain 2890L of permeate;
and (3) ultrafiltration: performing ultrafiltration on 2890L of the permeate which is subjected to microfiltration by a ceramic membrane by using ultrafiltration equipment, wherein the aperture of an ultrafiltration filter core is 3000Da, the membrane inlet pressure is 0.4MPa, and the membrane outlet pressure is 0.05MPa, so as to obtain 2800L of a small-molecular spirulina protein peptide solution with the molecular weight of less than 3000Da, and detecting the spirulina protein peptide solution, wherein the small-molecular peptide with the molecular weight of less than 3000Da is 97.3%;
and (4) nanofiltration: carrying out three-stage nanofiltration on 2800L of the ultrafiltration micromolecule spirulina protein peptide solution, wherein the aperture of an ultrafiltration filter core is 150-300Da, the membrane inlet pressure is 0.45MPa, and the membrane outlet pressure is 0.1MPa, so as to obtain 1100L of spirulina protein peptide desalted concentrated solution, and concentrating the ultrafiltration protein peptide solution by 2.55 times through nanofiltration, wherein the mineral removal rate is 84.1%;
spray drying and embedding: spray drying with a fluidized bed-pressure spray dryer, wherein the formula of the spirulina protein peptide spray drying feed liquid is as follows: 26.5 percent of spirulina protein peptide, 3 percent of maltodextrin, 5 percent of algin and 4 percent of arabic gum, and the pasteurization temperature of the feed liquid is 76 ℃ and the time is 60 minutes; homogenizing the sterilized feed liquid at the process homogenization pressure of 30MPa for 2 times, pasteurizing the feed liquid, homogenizing the feed liquid in a homogenizer at the spray pressure of 45MPa, using 2 spray guns, and performing spray drying at the air inlet temperature of 165 ℃ and the air outlet temperature of 75 ℃;
secondary drying: the powder outlet of the spray drying tower is connected with a fluidized bed for secondary drying, the air inlet temperature is 65 ℃, the air outlet temperature is 33 ℃, the water content of the spirulina peptide microcapsule powder is 3.64% through detection, the embedding rate is 94.1%, the preparation performance is good, and no fishy, salty and bitter tastes are generated;
vacuumizing and filling nitrogen for packaging: packaging with a vacuum-pumping nitrogen-filling packaging machine, wherein the packaging specification is 2kg aluminum foil bags, and the purity of the filled nitrogen is 99.9%.
Example 3
Pretreatment of a ceramic filter element: adding 6000L of spirulina protein peptide solution obtained by performing enzymolysis and decoloration on spirulina into a stainless steel storage tank, and pretreating by using tubular ceramic microfiltration equipment, wherein the aperture of a ceramic membrane is 0.1 micron, and the feeding pressure is 0.05MPa, so as to obtain 5820L of permeate;
and (3) ultrafiltration: ultrafiltering 5820L of the filtrate subjected to microfiltration by a ceramic membrane by using an ultrafiltration device, wherein the aperture of an ultrafiltration filter element is 5000Da, the membrane inlet pressure is 0.5MPa, and the membrane outlet pressure is 0.05MPa, thus obtaining 5700L of the small-molecule spirulina protein peptide solution with the molecular weight of less than 5000Da, and detecting the spirulina protein peptide solution, wherein the small-molecule peptide with the molecular weight of less than 3000Da is 96.9%;
and (4) nanofiltration: carrying out three-stage nanofiltration on a small-molecular spirulina protein peptide solution 5700L smaller than 3000Da, wherein the aperture of an ultrafiltration filter core is 150-300Da, the membrane inlet pressure is 0.5MPa, and the membrane outlet pressure is 0.1MPa, so as to obtain 2280L of spirulina protein peptide desalting concentrated solution, and concentrating the ultrafiltration protein peptide solution by 2.5 times through nanofiltration, wherein the mineral removal rate is 81.9%;
spray drying and embedding: spray drying with fluidized bed-pressure spray dryer. The spirulina protein peptide spray drying feed liquid formula comprises: 27.5 percent of spirulina protein peptide, 3 percent of maltodextrin, 5 percent of algin and 4 percent of arabic gum, and the pasteurization temperature of the feed liquid is 76 ℃ and the time is 60 minutes; homogenizing the sterilized feed liquid at the process homogenization pressure of 30MPa for 3 times, pasteurizing the feed liquid, homogenizing in a homogenizer at the spray pressure of 50MPa, and spray-drying at the air inlet temperature of 172 ℃ and the air outlet temperature of 75 ℃ by using 3 spray guns;
secondary drying: the powder outlet of the spray drying tower is connected with a fluidized bed for secondary drying, the air inlet temperature is 65 ℃, and the air outlet temperature is 32 ℃; through detection, the water content of the spirulina peptide microcapsule powder is 3.25%, the embedding rate is 91.1%, the preparation performance is good, and no fishy, salty and bitter taste exists;
vacuumizing and filling nitrogen for packaging: packaging with a vacuum-pumping nitrogen-filling packaging machine, wherein the packaging specification is 2kg aluminum foil bags, and the purity of the filled nitrogen is 99.9%.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (10)
1. An industrial method for refining spirulina protein peptide and spray drying microencapsulation is characterized by comprising the following steps:
s1, pretreatment of the ceramic filter element: adding a spirulina protein peptide solution obtained by carrying out enzymolysis and decoloration on spirulina into a stainless steel storage tank, and carrying out filtration pretreatment by using tubular ceramic microfiltration equipment;
s2, ultrafiltration: carrying out ultrafiltration on the pretreated feed liquid in the step S1 by ultrafiltration equipment to obtain micromolecule spirulina protein peptide;
s3, nanofiltration: carrying out three-stage nanofiltration on the micromolecule spirulina protein peptide obtained in the S2 by using ultrafiltration equipment;
s4, preparing spirulina protein peptide spray drying feed liquid: preparing the spirulina protein peptide obtained from the S3 into spirulina protein peptide spray drying feed liquid according to the following formula: 20-30% of spirulina protein peptide; 2-4% of maltodextrin; 3-5% of algin; 3-5% of Arabic gum;
s5, pretreatment of the feed liquid: pasteurizing the prepared spirulina protein peptide spray-dried feed liquid, and homogenizing in a homogenizer;
s6, spray drying and embedding: spray drying the pretreated spirulina protein peptide spray-dried feed liquid by using spray drying equipment, wherein the air inlet temperature is 135-175 ℃, and the air outlet temperature is 65-85 ℃;
s7, secondary drying: connecting a fluidized bed at a powder outlet of the spray drying equipment for secondary drying;
s8, vacuumizing and filling nitrogen for packaging: and packaging the powder obtained after secondary drying by using a vacuumizing nitrogen-filling packaging machine.
2. The industrial method for refining spirulina protein peptide and spray drying microencapsulation as claimed in claim 1, wherein the aperture of the ceramic membrane filter element used in the ceramic microfiltration equipment in S1 is 0.1-0.2 μm.
3. The industrial method for refining spirulina protein peptide and spray drying microencapsulation as claimed in claim 1, wherein the aperture of the membrane filtration core used by the ultrafiltration equipment in S2 is 3000-5000 Da.
4. The industrial method for refining spirulina protein peptide and spray drying microencapsulation as claimed in claim 1, wherein the aperture of the filtering core of the membrane used by the ultra-nanofiltration device in S3 is 150-300Da, and the continuous three-stage nanofiltration is performed on the small molecular spirulina protein peptide, the primary nanofiltration pressure is 0.35MPa, the secondary nanofiltration pressure is 0.4MPa, and the tertiary nanofiltration pressure is 0.5 MPa.
5. The industrial method for refining spirulina protein peptide and spray drying for microencapsulation as claimed in claim 1, wherein the maltodextrin, the algin and the acacia in the formula of the feed solution in S4 are all natural ingredients.
6. The industrial method for refining spirulina protein peptide and spray drying microencapsulation as claimed in claim 1, wherein the temperature for pasteurizing the feed liquid in S5 is 75-80 ℃ for 40-60 min.
7. The industrial method for refining spirulina protein peptide and spray drying microencapsulation as claimed in claim 1, wherein the homogenization pressure of the homogenization treatment in S5 is 25-40 MPa, and the number of homogenization times is 2-4.
8. The industrial method for refining spirulina protein peptide and spray drying microencapsulation as claimed in claim 1, wherein the spray drying equipment used in S6 is a fluidized bed-pressure spray dryer, the spray pressure is 40-60 MPa, the evaporation capacity is 200-500L/h, and the number of spray guns used is 1-3.
9. The industrial method for refining spirulina protein peptide and spray drying microencapsulation as claimed in claim 1, wherein the air inlet temperature of the fluidized bed in S7 is 55-77 ℃ and the air outlet temperature is 30-40 ℃.
10. The industrial method for refining spirulina protein peptide and spray drying microencapsulation as claimed in claim 1, wherein the specification of the vacuum-pumping nitrogen-filled package in S8 is 1-10 kg aluminum foil bag package, wherein the purity of the charged nitrogen gas is 99.7-99.9%.
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