TW201114372A - Embedding method for chlorophyll - Google Patents

Abstract

An embedding method for chlorophyll comprises: dissolving the chlorophyll in a solvent to form a solution; adding gel into the solution to obtain a gel solution; and drying the gel solution.

Description

201114372 VI. Description of the invention: [Technical field to which the invention pertains] The present invention relates to a method of embedding a chlorophyll. [Prior Art]

Most of the early pigment sources are taken from animals, plants and minerals. These natural substances are used as natural pigments. The science of the brain is used to produce artificial pigments by artificial synthesis. Synthetic pigments: fresh fructose Strong, stable, odorless, tasteless, soluble in water and adjustable in temperature. However, with the development of society and the people's birth of H Ping, and the scientific and technical pigments can not provide human nutrients; = the serious consequences of the consumption of artificial synthetic pigments. Therefore, there are restrictions or prohibitions on the use of synthetic pigments in the West. At the same time, people's pigments are becoming more and more interesting, especially in the consumption of natural color II (4) or pharmacological effects, thus increasing people's peace::: natural The research and application of pigments has also increased. Values, there are many kinds, the color is natural, many varieties have both nutritional value and some have the effect of medicine (such as vibrator yellow, safety a people trust. But ",, Wuyuan food color, its quality is not easy == From: the impact of natural resources and processing, sometimes c, heat, gas will have a value that greatly reduces the taste of the two private, high price and low pigment stability, due to 201114372 and the eight hair exhibition Different from the use of certain restrictions, the consumption of food / 廿 ..., pigment, and the pigmentation of the mouth pigmentation, and even the use of toxic ^ (four) has a certain degree of structure. Currently, development Tian. Ranxin, potential. Natural pigment instead of synthetic pigment has become the development trend of the food industry due to 4 dishes in the Changchun green day lack of color: =: was made -, : = (: = often make the vegetable after the ' It is directly dried by hot air, which can be used as a natural rhyme. The vegetable powdered vegetables will destroy the wind and dry vegetables and are not soluble in water. The color 'is extremely unstable to light, heat and oxygen, forming degradation products, making processing and storage process. The quality of the damaged cleavage is degraded. If the color changes, the appearance of the color is not applied to the food, the instability of the pigment, and the stability of the pigment is limited to the development of the green leaf based on the above-mentioned development, and it is necessary to enter the invention. For the purpose of the stability of solidification of 1 Weisu's package method. 钗回茱,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, - a method for embedding chlorophyll, which can be used for the storage stability of 彔 茱, '彔 。. 乂 201114372 包 包 14 14 14 14 14 14 14 14 14 14 14 14 14 14 乂 乂 乂 乂 14 chloro chloro chloro chloro chloro chloro chloro chloro chloro chloro chloro The chlorophyll is dissolved in a solvent, and the step is to add a soil material to the mixed solution to form a colloidal solution; and a drying step is to use a colloidal solution. From the absence of the [Embodiment], it is easy to understand, the miscellaneous and the gamma can be more clearly described in the detailed description of the method of 'in conjunction with the drawing', as shown in Figure 1, the embedding of the chlorophyll of the present invention Bulysis step S - Wall material addition step S2 and a drying bovine step: including the dissolution step S1, step S3. 1. The θ system makes 茱, the 彔 均匀 uniform> 谷 solution in a solvent, = two dissolved t the wall material The adding step S2 is formed in the mixed solution to form a colloidal dissolution, adding a wall solid energy servant, π α, and wood step S3, so that the colloid is dissolved and stored for storage test. The chlorophyll is carried out, and the chlorophyll is carried out. The embedding method step dissolving step S1: 5 ha dissolving step S1 is to start the leaf green ♦, too, every A / "pronunciation" is dissolved in a solvent. * , = ==:: Vegetables, then: In the solvent, form a mixed solution. The chlorophyll of the sputum dissolves the leaves evenly. The preferred cuisine is selected to be smuggled and removed. __, then select 201114372 to choose Foshuichuan for 30 seconds to 6 seconds. * If the enzyme is not activated and the enzyme is reduced to 4 enzymes in the green fun, the solvent will be added: the candied fruit = nutrients. Then, the amount of water to be injected is selected to be water, and the method is preferably selected as water in 10 ml of water, for example, the chlorophyll contained in the leaves of the present embodiment, and then The homogenization makes the sweet potato night. The sweet potato leaf material is removed from the crucible to form the mixed solution, and is uniformly dispersed in the mixed solution, and the $, , β, and the night are the core materials, and the wall material is added to the step S2. It is a violation of the common formation of microcapsules. ', combined with soil materials, wall material addition step release S2: = invented wall material addition step magic line as a core material, and add the wall in the - mixture solution = two mixed system selection will be - predetermined ratio of: The main ^ by m (four) for the formation of microcapsules of the outer film forming material: cellulose, wheat amygdino, ring paste with malt ^ / _ scales. In a preferred embodiment of the present invention, a mixture of dextrin, arsenic, and carterland is used as a microcapsule: a mixture of the mixture solution and the wall material is preferably a mixed solution of glutinous solution: Maltodextrin: Gum arabic: Cattle gum: Drinking water is mixed in a ratio of 6 ·] · 〇 · 3.2. For example, in this example, 60 ml of mixed S solution and 10 mg of maltodextrin are separately selected. 7 kg of gum arabic, 3 mg of cartland gum and 2 ml of drinking water to form a steroid solution to form the colloidal solution into a microcapsule form in the performance step. After taking the colloidal solution, the drying step S3 can be directly performed, or 2〇Ui4372 can be further as a homogenization step, and the homogenous strip of the step f is 1〇_, 15 seconds, and cooled for 1 minute until completely homogenized, The homogenization step can make the composition of the colloidal solution more minute and fine and the coating property of the microcapsule is high. J knife cloth 'k drying step S3: The drying step S3 of the present invention is a method of drying the colloidal solution by means of a dry cleaning method, a spray coating method, a dry coating method, an air material coating method, and a flowing bed package: The preferred embodiment is to select a hot air spray drying method, which is selected as the inlet temperature (10). c, the speed of the outlet extraction material is 5.5 millimeters per minute and NL/h (normally controlled every hour is the preferred trouble of the invention). The drying conditions are not limited by the drying of the application. condition. The S3, 'so that the _2 cut (four)_th S1 inner core material forms the microcapsule. In this way, the microcapsule can protect the influence, so that the material is not affected by the external environment, the microcapsule, the letter is less, the s is formed, and the chlorophyll is protected from the vaporization of the wall material. The chlorophyll embedding method can be transmitted by chlorophyll. Some of the chlorophyll components' further stabilize the leaf. The microcapsule leaf green ♦ destroys the wall material at an appropriate timing and releases the core material. The wall material was protected from the pigment change _, and it was confirmed that the chlorophyll was stabilized by the pigmentation of the chlorophyll. The pigment change of the microcapsule chlorophyll prepared by the method was tested. 201114372 To verify that the chlorophyll embedding method of the present invention can enhance the stability of chlorophyll, the test of pigmentation change. The pigment change test system is characterized in that the microcapsule chlorophyll is completed in the above-mentioned preparation conditions, and is referred to as a microencapsulation group, which is hereinafter referred to as a microencapsulated group, and the bottle contains a cerium oxide desiccant. The chlorophyll pigment changes of the microcapsule group were tested under different storage conditions. The storage conditions include conditions such as protection from light, different storage time and different storage temperatures, and test the retention of chlorophyll pigments in color and antioxidant capacity; another control group (not microencapsulated chlorophyll) For the control group, the pigment retention rate, color change and antioxidant capacity test were compared with the microencapsulated group to test whether the effect of microencapsulation on chlorophyll changes was observed. ~ Pigment retention rate test: The control group (not microencapsulated chlorophyll) and the microencapsulation group (the microcapsule chlorophyll of the present invention) were respectively stored under the conditions of being protected from light and natural light, and respectively subjected to room temperature treatment and 4 . After treatment, the chlorophyll retention rate was tested. The chlorophyll retention rate test method was as follows: The microencapsulation group was stored under different storage conditions, and 25 mL of 80% C was selected for extraction. The chlorophyll solution was filtered through a filter paper (NO.l, ADVANTEC 90 mm) and quantified to 50 mL, and then the chlorophyll extract of the final microencapsulated group was obtained, and the control group was also subjected to the above extraction to obtain a chlorophyll solution. Control group of chlorophyll extract. The microencapsulated chlorophyll extract and the chlorophyll extract of the control group were measured for absorbance at wavelengths of 647 and 664 nm, respectively, and the total chlorophyll content after storage was calculated by the following formula. 201114372

Chlorophyll a (mg / g) ^11^25(^664)--2.55(^647)1^ K

1000 xPF

Chlorophyll b (mg/g) = fe^l〇i^lZ)r4-9lU664)lxF

lOOOOxPF, total chlorophyll content (mg ♦ chlorophyll a + chlorophyll b. chlorophyll 80% 酉 酉 抽 抽 妒: sweet potato leaf powder weight (g) " 1 } The micro-encapsulation group of leaf green, amount. Leaf green to stomach + starting chlorophyll total command shaft cage and secret capsules & the total content of a green chlorophyll after occupation and the following formula calculated: Zhu Ding κ,,, heart 3, your chlorophyll retention rate ( %) Calculation = total content of Shuangling checksum ____ Total content of starting chlorophyll xl00% chlorophyll =;:, rate statistics, the results are shown in Figure 2, control === under sputum, compare (4) The retention rate of the primes is 47% of the loss of the control in the case of "臧 at 4 °C", and the microencapsulation group is only the preferred embodiment and the above control group and micro The capsules show that the fried oysters are really ―, and the dishes are tested. The results are as shown in Fig. 3, and the retention rate of leaf material is higher than that of control. In the fourth month, there is still about the interest group. Heterogeneous; while = 201114372 only 8% chlorophyll retention rate remained in the second month, and there was no chlorophyll retention rate by the third month. μ From the above pigment retention rate test, after the hair The chlorophyll retention rate of the microcapsules is obviously better, and the storage stoppers are better protected from light and stored at 4 ° C. Color change: preferred embodiment of the invention · taking the above-mentioned controlled chlorophyll extract and the micro The chlorophyll extract of the encapsulated group was tested and stored under the conditions of being protected from light and natural light, and the color change after treatment at room temperature and treatment was selected. The sample Lab value was selected by using a color difference meter (LA assist, CDM_G8, lapan). The L value is brightness (0~100). The higher the value, the brighter the color, the lower the value, the darker the color; the a and b values, the chromaticity of the gamma (4), +a: the table is not red, and the -a: indicates the greenness. , +b : indicates yellowness, _ b : indicates blueness, before the measurement, the white standard plate furnace 8〇46, γ==818〇, z=9l condition) 杈正' and calculate the hue angle by the following formula (As detailed as (4) and chroma (Chroma), a total of three repeated tests. Also hue angle stai ^ Cb / a) chroma = (a2+b2) U2 a hidden in the dark conditions and different temperature processing results as 4 Figure is not '4C after 4 months of storage, the color index of the control group decreased from 〇51 to 4242' decreased by 1 The microencapsulated group showed no significant decrease; after 4 months of storage at room temperature, the color index of the control decreased from Q51 to Q35, a decrease of 31/. The microcapsule group decreased from 〇·42 to 〇 4〇, a decrease of 5%, which is about 6 times the difference between the two. ^The results of storage in natural light conditions and room temperature treatment are shown in Figure 5. «The color index of the Xuan control group fell to 〇 in the third month, and the microencapsulation level fell from 0.42 in the fourth month of 201114372. To 0.32, a drop of 24%. It is known from the color change test described above that the lower the change in color tone is the microencapsulated chlorophyll treated by the method of the present invention, and the preferred storage conditions are dark and 4 ° C storage. Antioxidant ability test:

In a preferred embodiment of the present invention, the chlorophyll extract of the control group and the chlorophyll extract of the microencapsulated group are stored in a dark and natural light condition and subjected to room temperature treatment and 4 . (: After treatment, the test will increase the antioxidant capacity of chlorophyll after microencapsulation of chlorophyll, respectively, by testing a, a-diphenyl-p-picrylhydrazyl (DPPH) to scavenge free radicals (%), Ferric-Reducing Antioxidant P〇 Wer (FRAP) (mmol/100g) and total phenol content (mg/1 〇〇g). The experimental principle and the operation steps of the present invention are respectively described below: DPPH scavenging free radical ability (%) determination: DPPH free radical solution at 517 · With absorption peaks, when the DPPH receives the electrons provided by the anti-oxidation ride of the sample towel from the thiol group, it forms an electric deletion. + AH —DPPH:H + A.), and the absorption material disappears, == the antioxidant free in the age The ability of the base, when the ability to demonstrate hydrogen _, the higher the percentage of clearance. = The extract of the system and the microencapsulation group is sample 4, and 1 mL of freshly prepared estradiol solution is added. After a minute of shaking, the value of 5π is measured in minutes (10). The percentage of scavenging effect is obtained by the following formula. Long down 201114372 DPPH Clearance percentage L No sample control group absorbance value j x 100°/〇 FRAP measurement: Prussian blue generated by the action of sample and iron ions is used as the measurement. The reducing substance in the sample can reduce red blood salt to yellow blood salt, yellow blood and ferric ion to form Prussian blue, as shown below: K3Fe(CN)6 + sample — K4Fe(CN)6 + sampie _〇xide K4Fe(CN)6 + Fe3+ — Fe4[ Fe(CN)6]3 The amount of Prussian blue produced is used as a sample to reduce peroxide. The extract liquid of the above control group and the microencapsulation group was taken as 0.04 mL of the sample depleted liquid, and 〇. 12 mL 80°/ was added. Acetone and 1.2 mL of FRAP reagent were uniformly shaken. After 6 minutes of heat at 37 ° C, the absorbance at 593 nm was measured with a spectrophotometer. The FeS 〇 4.7H20 solution was used as a standard, and the green standard curve was used to convert the concentration of the sample reducing ability. The higher the absorbance value, the stronger the remote force of the sample. Determination of total phenol: In the phosphorus-phosphoric acid compound, phenol can reduce molybdenum. When the benzene ring in the sample is more, the phenol group reacts with F〇lin_Ci〇calteau, s phenol reagent, and the phenol of polyphenol compound The ring will reduce the hydroxyl group, and after the action, a blue-green complex can be produced. The higher the absorbance value, the more polyphenolic compounds are contained. The extract of the above control group and the extract of the microencapsulated group were taken as a sample and mixed with 0.1 mL of Folin-Ciocalteau, s-12-201114372 phenol reagent, and then kept at room temperature. After 5 minutes, add 2 mL of 15% Na2C03 and mix. Allow to stand for 30 min in the dark, and measure the absorbance at 750 nm. The total sample content is expressed as the dry weight per 100 g of sweet potato leaf powder in the extract (mg / 100 g). Use gallic acid as a standard and draw a standard curve. The results of antioxidant capacity test are shown in Table 1: Table 1. Antioxidant ability test and protection from light... Storage temperature Storage time DPPH scavenging free radical energy FRAP Total phenolic content (°C) (month) Force “%)_(mmol/100 g) (mg/100 g) 96.7±0.3 150.5 ± 0.3 7.32±0.04 83.5±0.3 119.5±1·5 5.69±0.08 67.5±0.3 78_1±0.8 3.96±0.11 51·6±0·4 52.2±0.6 2.52±0.04 40.3±0.3 41.8±0.4 2.05±0.05 81.3±0·3 57·3±0·7 3.17±0.03 79.7±0.4 54.7±0.3 2.77 士0.08 75·4±0.3 52.6 士0_9 2.63±0.07 70.6±0.9 49.5±0.6 2.36±0.04 67.7±0.4 45.9±0.7 2.25±0.01 ο 1234 ο 1234 0 12 3 40 12 3 4 ο 12 3 40 1234 96.7±0.3 150.5 士0_9 7.32 士 0.05 72.4±0.3 82.2±0.9 4.61±0.04 53.9±0.7 57.9士1.4 2.95±0.03 38.4±0.1 34.1±0·4 1.86±0.03 31·7±0_3 26.8±0.4 1·53±0_08 81.3±0.1 57.3±0.7 3.17±0·08 72.4 士 0.1 49·6±0_1 2· 34±0.04 65·8±0_6 43.4±0.8 1.98±0.05 58_6 ± 0.3 39.2±0.4 1_79 ± 0.05 55_1±0·3 36·1±0.3 1.58±0.01 96.7±0.3 150.5±0.5 7.32±0 32.7 ± 0.4 34.8 0.8 1.96±0.04 9.5±0·6 13.3 ± 0.2 0.74±0.02 non-detect 4.5±0.1 0·18±0_02 non-detect non-detect non-detect 81.3±0.4 57.3±0.4 3.17±0.01 67.5±0.5 45.4±0.4 2.27 士0.08 59 ·8±0'1 42.3±0.3 1.89±0.03 50.7±0.4 38.7±0.7 1.68±0.04 43_5 ± 0.1 34.1±0.2 1.35±0.01 Control group (4°〇) Microencapsulation group (4°〇) Control group (room Temperature) Microencapsulation group (room temperature) Natural light control group (room temperature) Microencapsulation group (room temperature) —13 — 201114372 It is known from Table 1 that the control group is under storage conditions during storage. The DPPH scavenging free radical capacity decreased from 96.7% to 40.3% from the month of the month to 4 months, and the decrease was 41.7%. The microencapsulation group decreased from 81.3% to 67.7%, but the decrease was only 16.7%. 'FRAP and total In terms of content determination, the shai control group decreased from 150.5 to 41.8 (mm〇l/100g) in the Bedding collection from 0 months to 4 months, a decrease of 72%, while the microencapsulation group was 57 3 To 45.9 (mm〇l/100g), the decrease was 20%; the total phenolic content was decreased from 7.32 to 2.05 in the control group from 7.0 to 2.0, a 72% decrease, and the microencapsulation group was 3.17 decreased to 2.25 (mg/100g), a decrease of 29%, and the FRAP and total phenolic content of the microencapsulated group were higher than the control group at the fourth month of storage. Under natural light storage conditions, the DPPH clearance rate was only 9.5%, FRAP was 13.3 (mmol/100g), and total phenol content was 0.74 (mg/100g) during the second month of storage. Without antioxidant capacity, no antioxidant capacity was detected by the fourth month. In the fourth month of storage, the DPPH clearance rate was 43.5%, and FRAP was 34.1 (mmol/100g) 'total phenolic content. It is 1.35 (mg/100g) and still retains antioxidant capacity. Based on the results of the above pigment change test, it was found that the storage condition had the greatest influence on the chlorophyll of the vegetable powder by "light", and the "temperature" was second, and the microencapsulated chlorophyll treated by the method of the present invention significantly slowed down the light and temperature. The effect will not be affected by external environmental factors, and the microencapsulated chlorophyll will be stored for 4 months when stored at a temperature of 4 ,, the chlorophyll retention rate is up to 86%, the color index is 0.40, DPPH free radicals. Clearing 201114372 • Rate 67.7%, FRAP 45.9 (mmol/lOOg), total phenol content 2.25 ~ (mg/IGGg), indicating that the microencapsulated chlorophyll treated by the method of the present invention can improve the storage stability. The above results are combined at μ, regardless of the pigment retention rate, color change, and antioxidant energy. The test towel is known to have the addition of a wall material, $ chlorophyll, which can increase the chlorophyll color effect and increase the storage stability of chlorophyll. Sex. " . The chlorophyll embedding method of the present invention is to form a chlorophyll-encapsulated method by using a wall material to form a chlorophyll, which can improve the stability of chlorophyll in the S] state. Gu (4) microcapsules protect chlorophyll, and achieve the effect of changing the color of the pigment when the chlorophyll is protected from the external environment. In the embedding method of the chlorophyll of the present invention, the protection of chlorophyll by microencapsulation can increase the storage stability. Although the present invention has been disclosed using the above preferred embodiments, the absence is not used.

2 The present invention is not limited to the spirit of the present invention. Various modifications and modifications to the above embodiments are still protected by the present invention. Therefore, the stipulations of Shuming’s bribery are attached. The scope defined in the scope of application for patent application shall prevail. 15 — 201114372 [Simplified description of the drawings] Fig. 1 is a flow chart showing the steps of a method for embedding chlorophyll according to the present invention. Fig. 2 is a view showing the presence or absence of microencapsulation of the preferred embodiment of the present invention, and the chlorophyll retention rate changes under different storage times and at different temperatures. Fig. 3 is a view showing the presence or absence of microencapsulation of the preferred embodiment of the present invention, and the chlorophyll retention rate changes under natural light conditions at different storage times and room temperature. Fig. 4 is a view showing the presence or absence of microencapsulation of the preferred embodiment of the present invention, and the chlorophyll color change in different light storage conditions and at different temperatures. Fig. 5 is a view showing the presence or absence of microencapsulation of the preferred embodiment of the present invention, and the chlorophyll color change under natural light conditions at different storage times and room temperature. [Main component symbol description] S1 dissolution step S2 wall material addition step S3 drying step

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Claims (1)

201114372 VII. Patent application scope: - 1. A method for embedding chlorophyll, comprising the following steps: a dissolving step of uniformly dissolving chlorophyll in a solvent to form a mixed solution; a wall adding step, Adding a wall material to the mixed solution to form a colloidal solution; and φ. a drying step for drying the colloidal solution to solidify the colloidal solution. 2. A method of embedding chlorophyll according to claim 1, wherein the wall material is a carbohydrate. 3. A method for embedding a chlorophyll according to claim 2, wherein the wall material is selected from the group consisting of gum arabic, alginate, starch, cellulose, maltodextrin, and cyclodextrin. And the group of Catalan gum. 4. A method for embedding chlorophyll according to claim 1, wherein the drying step is a spray drying method, a spray cooling drying method, a freeze drying method, or a calcium-alginite coating method. The colloidal solution is dried by one of air suspension method, flow bed coating method and co-crystallization method. 5. A method of embedding chlorophyll according to claim 1, wherein the solvent is water. 6. A method for embedding chlorophyll according to claim 1, wherein in the step of adding the wall material, after the wall material is added, a homogenizing step is performed on the colloidal solution to form the Colloidal solution.