KR100854692B1 - Functional beverages composing ginseng steamed red nano-powder under micron size - Google Patents

Abstract

A functional drink in which red ginseng nano particles of sub-micron size are dissolved in deep sea water rich in natural minerals and oxygen-rich water is provided to ensure excellent dispersity, mixing property and absorption property, to increase inorganic material and to be used as a functional health food, particularly a drink for preventing and improving immune-related disease. A functional drink contains red ginseng nano particles of sub-micron size, 12 to 150mg oxygen-rich water per a functional drink and deep sea water. The red ginseng nano particles of sub-micron size are obtained by the steps of; sterilizing at 90 to 100deg.C for 1 to 10min with steam under pressure and drying red ginseng; finely grinding the dried red ginseng in a first and second step; adding water to red ginseng powder and extracting; removing cellulose from the red ginseng extract with a centrifugal separator; removing a monomolecular material containing monovalent ions by a reverse osmosis membrane; and drying the red ginseng extract by a freeze dryer or a low temperature hot air dryer. The red ginseng nano particles have a size of 20 to 600nm and 5 to 25% moisture contents. The extraction is performed by adding 4 to 15ml water to 1g red ginseng, extracting at 50 to 86deg.C for 5 to 15hr and continuing the step 5 to 10 times. Deep sea water pumped from a depth of less than 200m below a sea level is diluted with 5 to 100 times their weight of purified water. The oxygen generating device adopts an absorption method or membrane method.

Description

Functional Beverages Containing Red Ginseng Nano-Powder of Submicron Size {Functional Beverages Composing Ginseng Steamed Red Nano-Powder Under Micron Size}

The present invention relates to a functional beverage containing red ginseng nanopowder of submicron size.

Korean ginseng is a medicinal herb that is toxic and unburned according to the ancient Chinese book of the rapid brewery (BC 33-48), Sanghanron (AD: 191-220), and Sinnonboncho (AD 483-496). Efficacy is described in the concept of 上 藥). Jeonchisam (田七 參) has been described as having a hemostatic effect on the herbaceous herb (本草綱目: AD 1598). The main active ingredient of ginseng is ginsenoside, and the chemical structure is elucidated and pharmacologically active ingredient is revealed through continuous research. The main characteristic of saponin, an active ingredient of ginseng, has a large lipolytic ability in the human body, promotes the absorption and digestion of nutrients, promotes metabolism by activating enzymes in cells, and promotes energy recovery by promoting serum protein and increasing energy. It is known to have an effect on improving anorexia, etc.

However, research on polysaccharides other than saponins is steadily in progress. Typically, ginseng polysaccharides, which are non-saponin-based materials, are complexes of high-molecular polysaccharides obtained by separation by the difference in molecular weight and ionic strength. These ginseng polysaccharides are known to have various effects such as hematopoietic promoting action, bone marrow defense action, cancer cell killing immune cell generation action, radiation sensitivity action, hypoglycemic action action.

Ginseng is divided into three types: fresh ginseng, red ginseng, and white ginseng, which maintain the original shape of ginseng. Because it contains moisture, it is prone to decay or damage during distribution, so long-term storage is difficult without special storage facilities or packaging.

Red ginseng is light yellow brown or light red ginseng that is dried by steaming without removing the reticulum of reticulum, which is a reticulum, which is also known as ginseng or white ginseng. It has been reported to have indirect effects on sexual behavior or reproductive effects, anti-inflammatory and anti-tumor effects, by acting on excitatory, hematopoietic and hypoglycemic, hepatoprotective, endocrine systems.

Meanwhile, the ginseng component contains active ingredients that inhibit cancer cell proliferation and formally induce normal cells. Anti-cancer activity and anti-cancer activity enhancing effect of anticancer agents have been suggested. It is reported that red ginseng has an active ingredient that inhibits cancer cell metastasis and anticancer drug resistance. G-Rh2, a red ginseng ingredient, causes so-called "Apoptosis" that kills cancer cells by itself and produces interferon. It is found that there is an effect that promotes. In addition, red ginseng extract has an effective effect on intracellular macrophage activation and antibody production.

The saponin component of red ginseng has the effect of promoting the relaxation of blood vessels, which plays an important role in the suppression of atherosclerosis and blood pressure control, and the protection of endothelial cells. Red ginseng has the effect of improving the red blood cell transformation ability to improve peripheral circulation. Red ginseng (saponin) has the effect of inhibiting the proliferation of vascular smooth muscle cells. Red ginseng has the effect of improving cholesterol metabolism (Korea-Japan Ginseng Symp, 1996).

In addition, red ginseng blood pressure lowering effect is associated with the action of promoting the vasorelaxation reaction of the red ginseng saponin component. Red ginseng improves quality of life (QOL) in patients with hypertension and is expected to be useful as adjuvant therapy in combination with hypertensives (M. Yamamoto, Ginseng Review. Vol 9, 1990).

Red ginseng has an ovarian function revival effect by improving microcirculation. Red ginseng is useful for improving female menopausal symptoms. Red ginseng (component) is useful for bone formation and improvement of bone biomechanical properties in experimental osteoporosis-induced animals ("Influence of Korean Red Ginseng Irradiation on the Physiological Functions of Elderly Female Rats", Korean Journal of Food and Nutrition, 2000 ). Red ginseng (saponin fraction) has the effect of inhibiting the development of stress ulcers and promoting the healing process of the symptoms of gastric ulcers caused.

Red ginseng contains ginseng as a raw material, and ginseng contains a large amount of saponin substance called ginsenoside, and each of these ginsenosides improves symptoms of central nervous system such as anxiety, insomnia, depression, and depression in addition to nourishing tonic. Efficacy in improving intellectual performance by improving cognition and memory, enhancing nonspecific bio-resistance against external harmful factors, normalizing living body against physical, chemical and biological external changes, and promoting blood glucose resistance and detoxification Ginseng because it has various pharmacological effects, such as protecting liver damage and promoting liver regeneration, enhancing cardiomyocyte protection and cardiovascular function, lowering blood cholesterol content, controlling blood pressure, and inhibiting cancer cell metastasis and resistance formation of anticancer drugs. Has been widely used as a herbal medicine since ancient times, but recently as a health food, various processed foods Is manufactured and consumed.

Red ginseng (紅 蔘) is a herbal medicine that is processed by numerical method, which is a traditional pharmaceutical technique, and is manufactured by special steaming, drying and processing process of ginseng. During this process, the starch particles in ginseng tissue are gelatinized, the tissue is firm, and various enzymes are inactivated to stabilize the quality. In addition, a secondary ingredient change occurs during the temperature treatment process to produce a unique active ingredient (ginsenosides Rs, Rg1, Rg2, Rh1, etc.) that do not exist in ginseng or white ginseng, and active ingredients such as antioxidant activity. The content is increased (Kitagawa et al. 1983, 1992). However, ginsenosides Rb1, Rb2, Rc, Rd, and Re, which are the major components of red ginseng, are unstable in the change of heat and extraction time when extracted for more than 10 minutes at high temperatures exceeding 100 ° C, especially diol-based ginsenosides. Its composition is known to change its form due to its weak heat resistance, which is easily converted into triol through hydrolysis reaction (Pak Myung-Han, Ph.D. .

In addition, ginseng contains 60 ~ 70% of carbohydrates such as starch, but it is a specific ingredient that cannot be found in other plants. Ginseng saponin (ginsenoside), polyacetylene (polyacety lene), antioxidant active phenolic compound, and high liver function Superstitions (gomisin-N, -A), insulin-like acidic peptides are included. The pharmacological effects of these ingredients are antidiabetic, anticancer, arteriosclerosis and hypertension, liver protection, gastrointestinal disease, hangover removal, anti-aging, anti-fatigue and anti-stress, brain activity, blood Circulation, immune function enhancement, anemia treatment, radioactivity defense, AIDS virus proliferation inhibition.

In addition, there are a number of processing companies that powder red ginseng in Korea, but most companies depend on cutter mills, roll crushers, and pin mills to achieve an average particle diameter of 150 µm. Currently, the research on the development of new products using red ginseng powder is very limited.In the industrial scale, red ginseng containing a large amount of plant fiber is hard to be applied compared to hard and brittle materials. It is difficult to prepare fine powder.

Red ginseng nano powder with an average particle size of 10 ㎛ or less has good dispersibility, mixing and absorbency, and it is a high value-added health food material such as drinks, tablets, capsules, cosmetic materials such as pharmaceutical materials, functional skin care and packs. Can be used. However, the production examples of red ginseng nano powder at home and abroad have not been presented so far.

On the other hand, as the public's demand for health has recently increased, there is a growing interest in water purifiers that can produce functional water, such as oxygen water, beyond simple water purification functions. In order to satisfy the demands of consumers, products in which oxygen of saturated concentration is dissolved in water or liquor are sold. Also, there are products in which oxygen water is added by adding oxygen to water purifier and cold and hot water. Conventional methods for producing oxygen water include a blower method that increases oxygen and dissolution rate by providing oxygen using an oxygen generator or a high-pressure gas container to produce oxygen and making it into microbubbles to increase dissolution rate. Pressurization method to raise the pressure, the dispersion method using the ejector principle is known. However, the pressurization method can increase the dissolution efficiency in the supersaturation concept, but there is a difficulty in applying a high pressure. The blower method and the ejector method are lost by rising upward through the bubble before oxygen is dissolved in water. There was a problem in that the efficiency is insufficient to produce supersaturated dissolved oxygen water.

In addition, the oxygen water purifier for producing oxygen water by such a conventional method for producing oxygen water requires a complicated device, which increases the size and weight of the water purifier itself, which is cumbersome to handle and install. In addition, the conventional oxygen water purifier must be equipped with a separate sterilizer or a separate filter in order to prevent secondary contamination by bacteria, the method of using an ultraviolet sterilizer, etc. in the conventional water purifier is bulky, expensive It is complicated to install, there is a risk of failure, and has a problem such as a long time required for sterilization is questioned the practical availability.

Common drinking water for human consumption includes tap water, purified water, natural water, boiled water, and distilled water. Recently, as the social atmosphere that puts importance on health has matured, many attempts to find clean and healthy water have been increasing, and accordingly, preference for purified water and natural water rather than tap water is increasing. However, the purified water has a problem that the beneficial minerals, etc., which are beneficial to the body are removed while passing through the filter, and the amount of dissolved oxygen is sharply lowered, and even in the case of natural water, its consumption is not much compared to the preference due to the distrust of environmental pollution. to be. Distilled water or boiled water is not able to supply oxygen to the human body in particular because all dissolved oxygen is not evaporated.

Dissolved oxygen present in the water can increase the blood oxygen concentration of the human body, such an improvement in blood oxygen concentration helps to make the human body healthier by performing the function of activating the human metabolism. In particular, insufficiency of the respiratory organs, respiratory failure of the skin, and oxygen deficiency due to excessive use of oxygen are known to cause cell insufficiency and cause various diseases or even death.

Therefore, various attempts have been made to increase the amount of dissolved oxygen present in the water. A conventional technique for preparing such oxygen water is a blower method that increases the dissolution rate by increasing the gas-liquid contact area by using an acid method using microbubbles. And a pressurization method for increasing pressure, a dispersion method using an ejector principle, and the like are known. Among them, the pressurization method can increase the dissolution efficiency in the supersaturation concept, but it is difficult for the general person to operate by applying a high pressure, and the blower method and the ejector method are floated upward through bubbles before oxygen is dissolved in water. Because of the loss, the oxygen dissolution efficiency is lowered, and thus there is an inadequate aspect for producing a high concentration of supersaturated dissolved oxygen water.

In particular, in the case of an adsorption method in which oxygen generated using an adsorbent is dissolved in water, since adsorbent dust is generated due to the use of the adsorbent, adsorbent dust harmful to the human body tends to be dissolved in water. There is a problem in that it is economically undesirable because a complicated configuration such as having various filters or tanks is required.

Therefore, there has been a demand for an oxygen water production apparatus capable of suppressing contamination while dissolving oxygen at high efficiency and high concentration, and a method for producing oxygen water capable of dissolving high concentrations of oxygen only by a simple process.

On the other hand, the deep sea water and surface sea water of 200 m or less have similar concentrations of salinity (NaCl) and most minerals, but nutrients (nitrogen nitrate, phosphoric acid, silicon), viable count, and water temperature have significant differences. The water temperature of deep sea water is almost constant throughout the year, and the surface water temperature of sea level is 16 ~ 28 ℃, but the water temperature of 374m deep water is 9 ℃, which shows low temperature stability, which is low in plankton, microorganisms, especially pathogenic bacteria. There is cleanliness. Deep seawater was not detected in the test of 10 kinds of bacteria including pathogenic Escherichia coli and pathogenic virus. The total viable count is clean water from 1/10 to 100% of surface water.

In addition, deep sea water contains about 5 to 10 times more inorganic nutrients than surface water and contains more than 70 different minerals, which can be harmful if consumed in large quantities. Deep mineral water, which contains only a small amount, has a deep relationship to human health, such as copper and zinc, which are essential trace elements. In addition, deep sea water is matured for a long time in the low temperature and high pressure of the deep sea, and the organic content is low and pH is lower than the surface water (around pH 7.8), and it is a group of water molecules compared to the land or mineral water of the land. ) Has a small characteristic.

Beverages are made by adding various additives, such as sweeteners, to natural, sprinkled or purified water. Beverage products include soft drinks, fruit drinks, sports drinks, juices, and the like, and various beverages are made according to the types and additives of the additives. However, mineral drinks that use natural water (mineral water), natural water or purified water that have spun underground water, or add specific ingredients may supply minerals of specific ingredients, but cannot supply various minerals necessary for the human body. In addition, the existing beverages can be ingested specific components as a functional beverage for health, but using natural water, natural water or purified water pumped from the basement is not functioning as a source of various minerals.

Deep sea water is originally aged for a very long time, which is rich in mineral nutrients necessary for supporting living organisms, is organic in nature and bacteria, and is clear, stable at low temperatures. Deep water generation shows that seawater in Greenland, Antarctica, and Vladivostok rapidly cools when it meets the glacier, and the cold, heavy seawater, which mixes with the salt that freezes out, does not go deeper into the ocean. Heavy streams descend from the depths of 200 m up to 4,000 m deep into the sea to form thick bands. Deep water that flows through deep seas does not mix with surface waters, like water and oil, due to temperature differences of more than 20 degrees. In the deep sea, where there is no light, only decomposing instead of photosynthesis, the deep water is rich in organic nutrients with various minerals. Table 1 compares the characteristics of deep ocean water at each intake area. Deep sea water consists of sources of origin and indigenous water produced at the intake area, and the composition of the intake area is slightly different. Therefore, each marine deep water intake zone is developing its own product. The source of deep water in the East Sea of Korea is the seawater of Vladivostok. The sedimented seawater of Vladivostok and the high water of the East Coast are combined to form the deep sea water inherent in the East Sea of Korea. It is distributed throughout the east coast such as Goseong, Yangyang, Gangneung, Uljin, and Ulleungdo, and has a slightly different characteristic from that of other deep sea waters in Japan and the United States. As shown in Table 1, the characteristics of deep sea water (goseong) in Korea are higher than that of Kochi, Okinawa, and Hawaii in the United States, and the contents of nutrients such as phosphoric acid and silicic acid are higher than those of Kochi or Toyama. More than that. In addition, dissolved oxygen was found to be higher than in other regions.

division Water temperature salt pH Nutrient Dissolved oxygen (mg / ml) phosphate nitrate Silicate East Sea Deep Water (Goseong) 1.52-1.9 33.72-33.94 7.19-7.90 1.7 ~ 4.3 3.6-13.3 72.0-108.0 9.13-9.47 Kochi Prefecture Deep Sea Water 8.1 ~ 9.8 34.3 ~ 34.4 7.8 ~ 7.9 1.1-2.0 12.1 ~ 26.0 33.9 ~ 56.8 4.1 ~ 4.8 Deep water in Toyama, Japan 1 ~ 2 34.1 7.74 1.2 ~ 1.8 14-26 30-38 5.0 ~ 6.0 Japan's Okinawa Prefecture Deep Water 9.0 34.71 7.93 3.05 21.89 44.81 US Deep Water in Hawaii 8.2-10.7 34.37-34.29 7.45-7.74 2.89-3.15 39.03-40.86 74.56 ~ 79.2 1.24-1.45

Thus, the present inventors conducted a study to broaden the application field of red ginseng powder, extract the finely ground red ginseng powder, remove the fibrin and remove the monomolecular substance, thereby maintaining a uniform particle size while maintaining the natural ingredients of red ginseng Red ginseng nano powder, Kochi Prefecture, Okinawa Prefecture, Okinawa, USA Hawaii's own deep sea water with a higher mineral content and dissolved oxygen than deep water in Hawaii and the above components are dissolved in oxygen water using the oxygen water production apparatus of Korean Patent No. 678489 The present invention was completed by developing a functional beverage.

It is an object of the present invention to provide a functional beverage in which red ginseng nanopowder is dissolved in deep sea water and natural oxygen-rich oxygen water.

In order to achieve the above object, the present invention provides a functional beverage comprising a red ginseng nanopowder of the sub-micron size, oxygen water containing a large amount of oxygen and deep sea water.

Hereinafter, the present invention will be described in detail.

In the functional beverage of the present invention, the size of the red ginseng nano powder is preferably 20 ~ 600 nm.

In addition, in the functional beverage of the present invention, the red ginseng nano powder is selected from red ginseng as a raw material and dried after moist heat sterilization; Ii) powdering the dried red ginseng by performing first coarse milling and second milling milling; Iii) extracting fine red ginseng by adding water to the powdered red ginseng powder; Iii) removing fibrin from the red ginseng extract by centrifugation; Iii) removing monomolecular substances containing monovalent ions by reverse osmosis from the red ginseng solution from which the cellulose has been removed; And iii) drying the red ginseng liquid from which the monomolecular substance has been removed, using a freeze dryer or a low temperature hot air dryer to prepare a nano powder as a solid, and removing the fiber of step iii). In addition, after the step, it is more preferable to include the step of removing the extra fiber, which is a polymer material, by filtration using a membrane in the red ginseng solution from which the fiber is removed, wherein the removal of the polymer material is a MF of 0.01 ~ 2 ㎛ size It is still more preferable to consist of a (micro filtering system) or an ultra filtering system (UF). In addition, the removal of the monomolecular material of step iii) removes the low molecular weight materials including pesticides using a nano filtering system (NF), and as a result, the monovalent ion exclusion rate is more preferably 70 to 95%. And iv) further comprising concentrating the red ginseng solution by reverse osmosis in the red ginseng solution from which the monomolecular material has been removed after the step of removing the monomolecular material, wherein the concentration is performed using a reverse osmosis system (RO). As a result, it is even more preferable that the monovalent ion exclusion rate is 90 to 99.9%. In addition, the red ginseng that is the raw material is more preferably contained 1: 1 ~ 0.2 ratio of red ginseng root: red ginseng, the wet sterilization of the step iii) is made for 1 to 10 minutes at a steam temperature of 90 ~ 100 ℃ Preferably, the drying of step iii) is preferably hot air circulation drying, and the dried red ginseng of step ii) more preferably has a water content of 5 to 25%, and the powdering of step ii) comprises red ginseng. It is more preferable that the final particle size is more than # 100, and the extraction of the micronized red ginseng of step iii) adds 4-15 ml of water to 1 g of red ginseng and 5-10 for 5-15 hours at 50-86 ° C. More preferably, the removal of the fiber in step iii) is preferably 50 to 75% of solid content by performing dietary fiber removal and dehydration using a centrifugal separator or a filter press.

In addition, the oxygen content of the oxygen water in the functional beverage of the present invention is preferably 12 to 150 mg per liter of functional beverage.

In addition, in the functional beverage of the present invention, the oxygen water is oxygen generating means for separating oxygen from the supplied compressed air; A contact membrane supplied with oxygen generated from the oxygen generating means and dissolved in water; And a first head receiving air and supplying it to the oxygen generating means; and a second head applying a vacuum to the oxygen generating means to increase the purity of the separated generated oxygen and simultaneously supplying it to the contact membrane under pressure. It is preferable that the oxygen generating means and the contact membrane of the oxygen water producing apparatus having a; two-head compressor comprising a; and the oxygen generating means and the contact membrane of the oxygen water producing apparatus is more preferably a porous membrane or a non-porous membrane, the oxygen water producing apparatus More preferably, the oxygen generating means and the contact membrane of the hollow fiber type or flat membrane type, the oxygen generating means of the oxygen water production device is more preferably employing the adsorption method or separation membrane method, the oxygen water production device is supplied with air Increase the purity of the separated oxygen generated by applying a vacuum to the compressor and the oxygen generating means supplied to the oxygen generating means At the same time, it is more preferable to further include a vacuum pump for supplying it under pressure to the contact membrane, and the oxygen water production apparatus is more preferably provided with an oxygen bottle for storing oxygen water obtained through the contact membrane, wherein the oxygen The water production apparatus is even more preferably provided with at least one of the oxygen water flow meter and the dissolved oxygen measuring device on one side of the oxygen can.

In addition, in the functional beverage of the present invention, the oxygen water is compressed air and supplied to the oxygen generating means; Supplying oxygen separated from said oxygen generating means to a contact film; And dissolving oxygen by supplying water to the contact film.

In addition, in the functional beverage of the present invention, the functional beverage is prepared by adding deep sea water taken from 200 m or less of water depth of 0.1 to 5 times the mass of red ginseng nano powder, and diluting it with purified water of 5 to 100 times. Preferably, the deep sea water of the East Sea contains more concentrated deep water or more preferably desalted deep water.

The functional beverage according to the present invention refers to a beverage in which deep seawater of deep seawater is added, a beverage in which deep seawater of concentrated east sea is added, a beverage in which deep seawater of desalted water is added, or a beverage in which mineral part is added to deep seawater of desalted water, etc. For example, soft drinks, fruit drinks, sports drinks, juices, etc. are mentioned.

The functional beverage of the present invention is prepared from nano-powdered red ginseng, oxygen water containing oxygen and deep sea water. Thus, the manufacturing process of the nano-powder red ginseng, the manufacturing process of oxygen water, and the treatment of deep water will be described separately.

<Production of Red Ginseng Nano Powder>

Since red ginseng contains a large amount of plant fiber and does not have high hardness, there is a limitation in reducing particle size by simply applying red ginseng to the red ginseng using a grinder, and this method also has a problem in that the particle size of the powder obtained is not uniform. . In addition, there is a problem of degeneration or contamination of the active ingredient due to friction between the red ginseng and the grinder. Red ginseng used in the present invention is a red ginseng powder having an appropriate average particle size # 120 or more through a grinding step of 2 to 5 steps by using an ultrafine granulation method. Specifically, 6-year-old red ginseng raw material is precisely weighed with a mixing ratio of red ginseng root and red rice ginseng as 7: 3. After the impurities are well selected, wet sterilization is performed for 3 minutes at a steam temperature of 90 to 100 ° C., followed by hot air circulation drying. The red ginseng raw material dried at 13% water content is put into a crusher hopper, and powdered to a final particle size # 120 or more through a first coarse milling process and a second fine milling process. When the red ginseng powder is tested for particle size, moisture, and microorganisms and passed approval, the product is shipped as a finished product through a sealing / labeling / packing process. Keep hygiene. The manufacturing technology of the red ginseng powder of the present invention having the above characteristics is shown through FIG. 1. Red ginseng as a raw material of the present invention uses red ginseng boiled and steamed with steam without peeling the epidermis.

Next, the present inventors efficiently remove the fiber from red ginseng to separate the red ginseng nanopowder to increase the yield, increase the purification rate to increase its physiological activity, and even mass production. First, red ginseng powdered by the above method is added to prepare red ginseng extract. Specifically, the red ginseng extract was prepared by adding 4-15 times of water to the weight of red ginseng powder and heating to 50-86 ° C. for 5-15 hours. In order to separate and dehydrate the fiber from the red ginseng extract thus extracted, the fiber was removed using a centrifuge or filter press. Thereafter, a micro filtering system and an ultra filtering system were used to remove high molecular weight proteins, carbohydrates, fibers and starch. In addition, in order to remove low-molecular substances such as pesticides, a nano filtering system was sequentially passed. In order to concentrate the red ginseng solution, the red ginseng solution is concentrated through a reverse osmosis system, and red ginseng nanopowder is prepared using a reduced pressure concentrator. The red ginseng nano powder is excellent in the content of polysaccharides, and excellent immune enhancing activity, the composition containing the red ginseng nano powder as an active ingredient can be used for the prevention and improvement of immune-related diseases.

In order to test the biological immunopotentiating activity of the red acid nanopowder of the present invention, lymphocyte proliferation ability, LAK cell production (tumor cell killing activity) and nitrogen oxide generation ability were tested. As a result, the lymphocyte proliferation ability of the red ginseng nanopowder high concentration tablet of the present invention was found to be 32.9 (500 ㎍ / ㎖) compared to the control 1.0 showed that the effect is significantly higher. In addition, LAK cell production (tumor cell killing activity) for the red ginseng nanopowder high concentration tablet solution of the present invention was 15.4 LU10 (100 ㎍ / ㎖) compared to the control, it was found that the effect is excellent.

Hereinafter, the manufacturing process of the red ginseng nano powder of the present invention will be described in detail.

1. The first step: red ginseng screening and drying after moist heat sterilization

6-year-old red ginseng raw material is precisely weighed with a ratio of 7: 3 of red ginseng root and red ginseng. After the impurities are well selected, wet sterilization is performed for 3 minutes at a steam temperature of 90 to 100 ° C., followed by hot air circulation drying.

2. Second process: grinding

The red ginseng prepared in the first step is put in a crusher and put into a grinder hopper, and then powdered to a final particle size # 120 or more through a first coarse milling process and a second fine milling process.

3. Third Process: Inspection Step

The red ginseng raw material dried at 13% water content is tested for particle size, moisture, and microorganisms, and when approved, the product is shipped as a finished product through the sealing / labeling / packaging process. Should be packaged in individual units and kept in a sanitary place in a waiting area.

4. Fourth step: manufacturing red ginseng extract

1 to 15 times the weight of the prepared red ginseng powder into water and heated to 50 to 86 ℃ for 5 to 15 hours to prepare a red ginseng extract.

5. The fifth step: removal of fiber

The red ginseng extract, which had undergone the fourth process, was separated using a centrifuge or a filter press to separate the extract from the fibrin, and also removed excess moisture. As a result, solid content reached 64%. In this case, it is preferable to remove the extra fiber, which is a polymer material, by filtration using a separator in the red ginseng solution from which the fiber is removed. The removal of the polymer material is a micro filtering system (MF) or UF (ultra) having a size of 0.01 to 2 μm. filtering system). At this time, the polymer material to be removed are proteins, carbohydrates, fibers, starch and the like and bacteria. At this time, the micro filtration device used is a microfiltration device for disinfection having a sieve size of 0.1 ~ 0.45 ㎛, the ultra filtration device uses an ultra filtration device of 10 ~ 500 K-Dalton size.

6. The sixth step: removing monomolecular substances

Reverse osmosis removes monomolecular substances containing monovalent ions, and removes residual heavy metals and pesticides using a nano filtering system and removes low molecular substances. At this time, the nano filtration device using a 0.5 K-Dalton size of the nano filtration device to remove the low molecular weight material of 500 or less molecular weight. As a result, 99% or more of impurities are removed and monovalent ion exclusion rate reaches 70 to 95%.

Additionally, after removal of the monomolecular substance, the red ginseng solution is concentrated by reverse osmosis, the concentration is concentrated using a reverse osmosis system (RO), and the monovalent ion exclusion rate reaches 90 to 99.9%.

7. Step 7: Drying to Solid

The red ginseng extract was finally dried with a freeze dryer or a low temperature hot air dryer to prepare a red ginseng nanopowder having a size of less than micron.

<Production of Oxygen Water>

As the oxygen water of the present invention, the oxygen water producing apparatus and the method described in the registered patent No. 678489 was used.

The apparatus for producing oxygen water used in the present invention may additionally antibacterial treatment of the oxygen generating means and the contact membrane, which may be achieved by installing an ultraviolet lamp or an antibacterial sponge at the front or the rear of the feed water.

In addition, the method for producing oxygen water is compressed air and supplied to the oxygen generating means; Supplying oxygen separated from said oxygen generating means to a contact film; And dissolving oxygen by supplying water to the contact film.

Hereinafter, with reference to the accompanying drawings will be described in more detail the oxygen water production apparatus of the present invention.

Figure 4 is a schematic system of the oxygen water production apparatus used in the present invention.

The oxygen water producing apparatus is basically provided with oxygen generating means (2) for generating oxygen from the supplied air and a contact film (1) for dissolving high concentrations of oxygen supplied from the oxygen generating means in contact with water to dissolve it. .

As the oxygen generating means 2, conventionally known oxygen generating means can be used without limitation, and for example, an adsorption method or a separation membrane method can be used. In the case of the adsorption method, dust is inevitably generated due to the use of the adsorbent, but according to the present invention, a separate means for dissolving oxygen in water is used after the adsorption method employing such an adsorbent. In 2), the dust can be naturally filtered, so no separate dust removal device is required, so the adsorption method using an adsorbent can be freely used without any limitation.

As shown in FIG. 6, the basic principle of the adsorption method is to first adsorb nitrogen, which is a high selectivity component, while compressed air passes through an adsorption tower filled with an adsorbent such as a molecular sieve under high pressure, and oxygen of low selectivity is first adsorbed. Passes through and is discharged out of the adsorption tower. The compressed air through the compressor can be cooled as needed to increase the adsorption power or to remove moisture. The cooled air is introduced into one of the two adsorption towers, and the nitrogen molecules in the air are different from the oxygen molecules due to the difference in adsorption power. First adsorbed. When the adsorbent in the adsorption tower is saturated by the nitrogen molecules, the solenoid valve is operated by the electronic control device that senses this, and the compressed air is transferred to another adsorption tower. At the same time, the adsorption tower saturated with nitrogen gas is desorbed by purging for regeneration. By performing the discharge is to be discharged through the nitrogen control valve (10). This process can be repeated to continuously produce oxygen.

This repetition process is performed every few tens of seconds, and the adsorbent is repeatedly adsorbed and desorbed.

As the oxygen generating means 2, a separation membrane method can be used in addition to the adsorption method. Such a separator may be selected from a plate type and a hollow fiber type as a porous or nonporous gas separator.

As an example of the basic principle of the separation membrane method, it is due to the difference in the permeability of each gas component in various gas mixtures permeating through the membrane. As shown in FIG. 5, the compressed air supplied to the separator is composed of various gas mixtures including oxygen and nitrogen, and the gas mixture is in contact with one side of the separator to selectively permeate at least one of the gas components. .

Gas components selectively permeated by the membrane must pass faster than at least one gas component of the gas mixture.

Relatively impermeable gas components must penetrate the membrane more slowly than one or more components of the gas mixture. By this principle, the gas mixture is separated into two streams, which are selectively permeate gas streams and those which are not permeate gas streams. Therefore, in order to properly separate the gas mixture, a film forming material having a high permeability for a specific gas component should be selected.

In order to selectively separate and concentrate the mixed gas, the gas separation membrane generally has an asymmetric structure composed of a dense separation layer on the surface and a porous support on the bottom. The selective separation ability of the mixed gas for a particular membrane forming material depends on the degree of compaction of the separation layer, and the flow rate of the selectively separated gas depends on the thickness of the separation layer and the degree of porosity of the porous support, the substructure of the asymmetric membrane. . In order to selectively separate the mixed gas, the surface of the separation layer should be as free of defects as possible, and the pore size should be 5 mm or less. In addition, the separation layer should be as thin as possible to obtain high gas permeability because the gas permeability is inversely proportional to the effective membrane thickness. In order to minimize resistance to gas flow selectively passing through the separation layer, the asymmetric membrane substructure should preferably have a porous structure. As the form, it can manufacture into a hollow fiber type, a flat membrane type, etc.

Oxygen generated by the oxygen generating means 2 such as the separation membrane or the adsorbent is moved to the contact membrane 1 to be dissolved by contact with water. The other structure of the contact membrane 1 can pass the liquid to the outside or inside of the tube, at which time it is dissolved by supplying concentrated oxygen from the opposite side. Oxygen generated from the oxygen generating means 2 is introduced through the contact membrane 1, and at this time, bubbles are not generated, and oxygen passed through the contact membrane 1 is dissolved in a liquid such as water by diffusion. It means. In the present invention, by using such a contact membrane (1) it is possible to simply dissolve the oxygen of the molecular unit in water to simply increase the content of dissolved oxygen and also increase the dissolution time. In particular, there is an advantage that the installation of the injector, which is used in the prior art, the pressure of the vessel, the temperature of the vessel is not required.

In the case of the contact membrane 1, it is more preferable to allow the flow of liquid to the inside of the tube in consideration of the influence of the contact area and the flow velocity, and the efficiency tends to decrease when the contact membrane 1 is long. It is preferable not to form too long. It is also preferable that the contact film 1 be formed in a corrugated structure in order to increase the surface area in contact with the liquid. In addition, both porous and non-porous are possible, and in order to induce gas diffusion more appropriately, a non-porous membrane is preferable. As the form, it can manufacture into a hollow sand shape, a flat film shape, etc. As the material, a film made of polysulfone, polysulfone composite, polypropylene, polyethylene, polyethersulfone, polyimide, teflon, silicone, polytetrafluoroethylene and a mixture of two or more thereof can be used.

A liquid such as water flows into the contact membrane 1 through one end thereof. In this case, a water pressure measuring device 6 is mounted on a part of the pipe to measure the water pressure. By discharging the inflow water through 5) it is possible to ensure that the appropriate water pressure always flows into the contact membrane (1).

A separate compressor and a vacuum pump may be used to supply compressed air to the oxygen generating means 2. That is, a compressor for compressing and supplying air to the oxygen generating means 2, and a vacuum pump for inducing high-purity oxygen generation by applying a vacuum to the oxygen generated from the oxygen generating means and supplying it to the contact film 1 It may be provided separately.

As another method, the present invention provides a first head for supplying compressed air obtained by compressing air and compresses it, and vacuums oxygen generated from the oxygen generating means to generate higher purity oxygen and at the same time, the contact membrane It is more preferable to use a two head compressor 3 having a second head which is fed under pressure in comparison with using them separately. Because such a two-head compressor (3) can use the pressurization and vacuum at the same time, even in low pressure operation to induce a pressure difference with one compressor to the maximum, compressed air and / or to the oxygen generating means (2) and the contact membrane (1) By allowing concentrated oxygen supply at the same time, it is possible to generate a high concentration of oxygen from the oxygen generating means 2, which is less than the concentrated oxygen generated in the oxygen generating means 2 by passing through the contact membrane 1 under pressure. It generates oxygen with a purity of about 10% higher, which allows this high purity oxygen to be dissolved in water in the form of a single molecule. In addition, the use of only one compressor can reduce the amount of power used, it is preferable to reduce the amount of noise generated during operation.

A pressure gauge 8 is attached to the output of the first head of the two-head compressor 3 to measure the pressure of the compressed air supplied to the oxygen generating means 2 to maintain an appropriate pressure. A pressure gauge 7 is also attached to the output unit, so that the appropriate pressure can be induced by measuring the pressure of concentrated oxygen generated from the oxygen generating means 2.

The apparatus for producing oxygen water according to the present invention may further include an oxygen bottle 4, which may serve to store oxygen water discharged from the contact membrane 1. The oxygen can 4 is also provided with an oxygen water flow meter 11 and / or dissolved oxygen measuring device 12 to control the operation of the oxygen water generating system. That is, when the oxygen water supply is excessive, the oxygen water flow rate measuring device 11 detects this and stops the oxygen water generation system. In particular, by measuring the content of dissolved oxygen by the dissolved oxygen measuring device 12, it is possible to appropriately control the system by the automatic control device to have an appropriate oxygen content according to the intended use.

As such dissolved oxygen measuring apparatus 12, the oxygen sensor generally used can be used without a restriction | limiting, for example, there is a galvanic type. The galvanic type is an electrochemical oxygen sensor, in which a reduction reaction occurs at a noble metal electrode and a reduction current is generated according to the contacted oxygen concentration, and is a representative oxygen sensor that can be used within a temperature range of room temperature. It is widely used for low oxygen analysis.

Such an oxygen bottle 4 can be more usefully applied especially to a device such as a water purifier. That is, after storing the oxygen water in the oxygen can (4), it is convenient because it can be provided by cooling or warming through an appropriate method. Since the oxygen water is mostly used for human consumption, it is preferable to purify the water as much as possible, and it is preferable to purify the supply water before supplying the oxygen water production apparatus, that is, the contact membrane 1. In the case of purifying oxygen water after preparation, dissolved oxygen may be removed by various filters, which is not preferable.

It is preferable to perform an antibacterial treatment on the manufacturing apparatus so that bacteria do not propagate in oxygen water, and the photocatalyst, for example, may be used for the oxygen generating means 2, the contact membrane 1, the housing, the oxygen can 4, and the piping. Treatment with TiO ₂ , WO ₃ , ZnO, SiC, CdS, etc., or treatment with silver nanoparticles to impart antimicrobial properties, can be antimicrobial treatment through UV sterilization or antibacterial sponge during the supply of the feed water. Alternatively, the antimicrobial treatment may be performed by irradiating an ultraviolet lamp to the oxygen bottle itself. Such antibacterial treatment further increases the safety and stability of the oxygen water.

The oxygen water production process will be described in more detail according to the process sequence as follows.

First, the air compressed through the first head of the two-head compressor 3 is supplied to an oxygen generating means 2, for example, an adsorption tower or a separator, to generate a high concentration of oxygen. For example, nitrogen is discharged to the outside through the control valve (10). The high concentration oxygen separated by the oxygen generating means 2 is formed into oxygen of higher purity by vacuum through the second head of the two-head compressor 3, and is injected into one end of the contact membrane 1 under pressure. do. The injected high concentration of concentrated oxygen is dissolved in contact with the liquid such as water introduced to the other end of the contact film 1 by diffusion on the contact film. Oxygen water dissolved in a high concentration of oxygen is introduced into the oxygen can (4), the circulation process as described above is properly controlled by the oxygen water flow rate measuring instrument 11 attached to one side of the oxygen can (4). In addition, the content of the oxygen can also be appropriately controlled by the dissolved oxygen measuring device 12 attached to one side of the oxygen can (4).

Oxygen water generated by such a process can be used in water purifiers and the like, it can be used as a functional beverage such as the present invention. In addition, it can be used as a raw material of alcoholic beverages such as alcohol, ice creams such as ice cream, beverages and the like. In particular, high-quality oxygen can be dissolved in the water at a high concentration, thereby providing the user with a more useful healthy oxygen water capable of supplying oxygen to the body smoothly.

<Treatment of East Sea Deep Water>

Fill the container with deep sea water and place it in a sterilization chamber to sterilize the filling. At this time, the sterilized deep sea water is 10% or less in the beverage, the preferred range is 5% or less, the optimum range is 3%. It is preferable to mix other sweeteners and other additives with the deep sea water in advance.

Alternatively, the deep sea water concentration is concentrated by reverse osmosis, electrodialysis or evaporation. As the concentrated deep sea water, the range of the beverage is 5% or less, and the preferred range is 3% or less.

Alternatively, deep sea water can be desalted. At this time, desalination is carried out by at least one treatment method from an electrodialysis method, a reverse osmosis membrane method, an ion exchange membrane method and a distillation method. At this time, the beverage adoption range of the deep sea deep water is 1-99%.

Alternatively, the deep sea water of East Sea is evaporated by heat evaporation, vacuum evaporation, sun drying, and ventilation drying, and the evaporated dry matter is mixed with natural water, natural water or distilled water pumped underground, and filled into the container and sterilized. Put in a thread and sterilize the filling. Here, the range of employment for the beverage of evaporated dry water of deep sea water of East Sea is 2% or less, and preferably 0.05-0.5%. If it is 2% or more, too much salt is contained and it is not suitable as a drink.

Alternatively, sterilize the filling by filling the container with juice and concentrated deep sea water. When diluting the juice or the concentrated East Sea deep water with natural water, it is preferable that the salt concentration of the juice be within 5% depending on the amount of natural water used.

Another method is to sterilize the filling by filling the vessel with desalted deep sea water and sweeteners or other additives. The range where the desalination copper deep water can be employed is 1 to 99%, the preferred range is 5 to 50%, and the optimum range is 10 to 30%.

Another method is to concentrate the desalted deep sea water, fill the container with concentrated deep sea water and sweeteners or other additives and sterilize the filling. The range where the desalination-concentrated East Sea deep water can be employed is 1 to 99%, the preferred range is 2 to 50%, and the optimum range is 5 to 30%.

In another method, the desalted deep sea water, mineral salts, and other components other than the minerals are filled into a container, and the filling is sterilized.

The red ginseng nanopowder, which is one component of the present invention, has a maximum particle size of 600 nm or less, an average particle size of less than 300 nm, and is composed of a uniform particle size, and thus has good dispersibility, mixing property, and water absorption. One component of oxygen water is oxygen water which has no fear of dust generation by adopting contact film, and oxygen water with improved dissolved rate and dissolved time of oxygen. Deep sea water, another component, has low temperature stability and inorganic substances such as nitrogen, phosphorus and silicic acid. It is rich in nutrients and contains clean redness and stable maturation that are not contaminated with common bacteria or chemicals such as Escherichia coli, and essential trace elements and various minerals, so it contains these red ginseng nano powders and deep sea water and oxygen The beverage of the present invention is useful as a functional health food that prevents and maintains health from various diseases and diseases, in particular, cotton It can be used as for the prevention and improvement of disease-related drinks.

Hereinafter, the present invention will be described in more detail based on the following examples. However, the following examples are only for illustrating the present invention, and the present invention is not limited to the following examples and may be changed to other embodiments equivalent to substitutions and equivalents without departing from the technical spirit of the present invention. Will be apparent to those of ordinary skill in the art.

Example 1 Preparation of Red Ginseng Nanopowder

The present inventors first prepared the red ginseng nano liquid by the following process. Red ginseng nano liquid refers to a liquid prepared by extracting red ginseng powder with water in a certain temperature range for a predetermined time, and then filtering the extract using an ultrafiltration membrane and then again concentrating the nano membrane or reverse osmosis membrane. The red ginseng nano liquid contains a large amount of human active ingredient (mainly saponin material), and the particle size of the nano powder has a particle size of up to 600 nm.

Specifically, the inventors weighed 6-year-old red ginseng raw materials with a mixing ratio of red ginseng root and red rice ginseng at 7: 3, and carefully selected impurities, followed by wet sterilization at a steam temperature of 95 ° C. for 3 minutes, followed by hot air. Circulation drying. The red ginseng raw material dried at 13% water content was put into a mill hopper, and powdered to a final particle size # 120 or more through a first coarse milling process and a second fine milling process. Particle size, moisture, and microbial tests were performed on the prepared red ginseng powder to check for abnormalities.

Then, 150 g of red ginseng powder prepared above and 1000 g of purified water were added to a three-liter extraction device consisting of a heater, a condenser, an impeller, and a thermometer, and extracted at 80 ° C. for 9 hours. 1000 g of purified water was added to the solids, followed by extraction at 80 ° C. for 9 hours to obtain a secondary extract using a centrifugal filter. In the same manner, all the extracts obtained seven times were mixed to obtain 6900 g of red ginseng extract. The red ginseng extract was filtered using an ultrafiltration membrane (fraction molecular weight 300,000) in a continuous operation (after membrane separation pressure 1.5bar, 5 hours) to obtain 6555 g of red ginseng filtrate 95% of the red ginseng extract. In order to concentrate the red ginseng filtrate, using a reverse osmosis membrane with a salt removal rate of 99% (back end pressure 9bar, 7 hours) to 70% compared to the red ginseng filtrate to obtain 2070 g of the final red ginseng nano liquid.

The red ginseng nanoliquid was finally dried with a freeze dryer to prepare 30 g of red ginseng nanopowders having a size of less than micron (FIG. 1).

Example 2 Preparation of Oxygen Water

An oxygen water production apparatus as shown in Figure 6 was prepared. First, 1 kg / cm 2 of compressed air is injected into the oxygen generating membrane 2 (non-porous polysulfone composite membrane, diameter 48 mm * length 182 mm, effective membrane area 0.58 m 2) through the first head of the two-head compressor 3. The concentrated oxygen having the oxygen purity of about 42% was drawn through the second head, which is the other head, to the vacuum of 400 mmHg. It was added to the porous polysulfone composite membrane, diameter 70 mm * length 232 mm, effective membrane area 1.58 m 2). At this time, when the water pressure applied to the contact membrane is 1.5 kg / ㎠ or more to control the flow rate using a bypass valve.

The dissolved oxygen amount was measured by a dissolved oxygen measuring instrument (manufactured by Maxtec) at an experiment temperature of 25 ° C. and a concentrated oxygen supply pressure of 1 kg / cm 2, and 34 mg / l was obtained. The change according to the pressure at 25 ° C. was shown in Table 2 below. The change in dissolved oxygen with time is shown in Table 3 below.

Pressure (㎏ / ㎠) 0.2 0.4 0.6 0.8 1.0 1.2 1.4 Dissolved Oxygen Amount (mg / l) 14 19 24 29 34 38 41

Minutes 5 10 15 20 25 30 Dissolved Oxygen Amount (mg / l) 34.0 33.0 32.7 32.5 32.4 32.4

Oxygen water production apparatus according to the present invention as can be seen from the results of Table 2 and Table 3 is dissolved oxygen in the water in the water due to the use of the contact membrane simply because the dissolved rate increase and the dissolution time can be increased oxygen The number can be produced more efficiently. In addition, in order to generate oxygen in general, a pressure of 2 kg / cm 2 or more should be applied. However, in the present invention, a pressurization and vacuum of about 1 kg / cm 2 may be simultaneously used using a two-head compressor, so that a single compressor may be used even in a low pressure operation. By inducing pressure difference as much as possible, it is possible to simultaneously supply oxygen and concentrated oxygen to the contact membrane, thereby reducing power consumption and noise during operation.

In particular, in the case of general tap water, the dissolved oxygen amount is only 6 to 10 mg / l at room temperature, and the conventional method takes a method such as installing an injector, strengthening the pressure of the vessel, and lowering the temperature of the vessel to increase the oxygen dissolution rate. Dissolved oxygen amount can be supplied 14 to 41 mg / L, or more oxygen water without a separate oxygen dissolved device. As a result, when using the oxygen water production apparatus according to the invention it is possible to increase the dissolved time as well as increase the oxygen dissolution rate when applied to the existing additional equipment.

In addition, the oxygen dissolution rate can be further increased when the water temperature is reduced, and the area of the separation membrane and the contact membrane can be increased according to the capacity to provide an oxygen water supply device that is easy for the user to use.

In addition, when the pressure on the supply side is increased to 1 kg / ㎠ or higher or the vacuum degree is increased, the dissolved oxygen amount is raised to 70 mg / L or higher at room temperature and atmospheric pressure to drink high concentration of oxygen. It becomes possible. In addition, the existing oxygen water purifier is concerned about the propagation of bacteria as the water stays in the device, but the present invention can overcome this problem by giving a sterilization function.

<Examples 3 to 11> Treatment of Deep Sea Water

Example 3) A deep water was poured into a container with natural water pumped from the basement and the container was placed in a sterilization chamber to sterilize the filling. Here, the natural water and the deep sea water can be individually filled, but they can also be mixed in advance and filled. As the deep-sea deep-sea water, the range of employment is 10% or less, and the preferred range is 5% or less, and the optimal range is 3%.

Example 4) Containers were filled with natural water sprinkled underground and deep sea water, sweeteners and other additives. The vessel was then placed in a sterilization chamber and the filling was sterilized. Here, the natural water and the deep sea water may be individually filled, but may be pre-mixed and charged in a mixed state. Sweeteners and other additives are preferably mixed with natural water or deep sea water in advance. As the deep-sea deep-sea water, the range of employment is 10% or less, and the preferred range is 5% or less, and the optimal range is 3%.

Example 5) A container filled with natural water sprinkled underground and deep seawater concentrated in East Sea was placed in a sterilization chamber, and the filling was sterilized. Here, the deep sea water concentration is concentrated by reverse osmosis, electrodialysis or evaporation. As the concentrated deep sea water, the range of the beverage is 5% or less, and the preferred range is 3% or less.

Example 6 A container was charged with deep water from the basement and deep-salted East Sea deep water and placed in a sterilization chamber to sterilize the filling. Here, desalination is based on at least one treatment method from an electrodialysis method, a reverse osmosis membrane method, an ion exchange membrane method, and a distillation method. The beverage adoption range of the deep sea water is 1-99%.

Example 7) The deep sea water of the East Sea is evaporated by heating evaporation, vacuum evaporation, sun drying, ventilation drying, etc., and the evaporated dry matter obtained is mixed with natural water, natural water or distilled water pumped underground, and filled into the container, and the container is sterilized. And the filling was sterilized. Here, the range of employment for the beverage of evaporated dried water of deep sea water in East Sea is 2% or less, preferably 0.05-0.5%. If it is 2% or more, too much salt is contained and it is not suitable as a drink.

Example 8) Juices and concentrated East Sea deep water were charged to a vessel. The vessel was then placed in a sterilization chamber to sterilize the filling. When diluting the juice or the concentrated East Sea deep water with natural water, it is preferable that the salt concentration of the juice be within 5% depending on the amount of natural water used.

Example 9 A container was filled with natural water sprinkled underground, deep water desalted, deep sweetened water and other additives. Next, the container is placed in a sterilization chamber to sterilize the filling. The range where the desalination copper deep water can be employed is 1 to 99%, the preferred range is 5 to 50%, and the optimum range is 10 to 30%.

Example 10) The container was filled with concentrated deep seawater and desalted East Sea deep water, and sweeteners and other additives. The vessel was then placed in a sterilization chamber to sterilize the filling. The range where the desalination-concentrated East Sea deep water can be employed is 1 to 99%, the preferred range is 5 to 50%, and the optimum range is 10 to 30%.

Example 11 The container was filled with the natural water and the desalted deep-sea water, inorganic salts, and minerals which were raised underground. The vessel was placed in a sterile chamber and the filling was sterilized.

It is not limited to the said example using such deep-sea deep-sea water. For example, tap water, river water, or distilled water can be used as a substitute for natural water pumped up underground. In the case of desalting deep sea water, it is preferable that the deep sea water is desalted by at least one treatment method from an electrodialysis method, a reverse osmosis membrane method, an ion exchange membrane method, and a distillation method, leaving only components close to the body fluid. Concentration is dried by reverse osmosis, electrodialysis, evaporative concentration, etc. by heating evaporation, vacuum evaporation, sun drying, and ventilation drying. Other additives may be used to produce a soft drink using carbonic acid, or a beverage that increases dissolved oxygen by blowing oxygen into the desalted deep sea water. Demineralized water can also be used to make liquor such as beer, shochu, makgeolli, and fruit wine. As a means for filling the container of the beverage of the present invention, there are various means such as a method of filling each of the deep sea water and mineral water, etc., or mixing them in advance and filling them in a mixed state. Moreover, sterilization by hot steam, infrared rays, radiation, etc. are used as a filler sterilization means, for example.

<Example 12 to Example 14> Red ginseng beverage production 1

The present inventors prepared the red ginseng nano liquid having a red ginseng nano powder of 0.5 to 20 wt%. Red ginseng nano liquid having a red ginseng nano powder of 3 to 8 wt% is preferable. The deep water may be contained in an amount of 0.5 to 8 wt%, preferably 1 to 5 wt%. Oxygen water may be contained in the red ginseng beverages with an oxygen content of 12 ~ 150 mg / L, preferably contained in 25 ~ 100 mg / l.

Specifically, the red ginseng nano liquid obtained by the extract solution method of Example 1 and mixed with water according to the composition of Examples 12 to 14 of Table 4 (using Mixer), that is, the technical method of patent PCT / KR2006 / 001774, that is, membrane contactor Red ginseng drink was added to the oxygen (oxygen pressure 3bar, liquid pressure 2.5).

<Example 15 to Example 17> Red ginseng beverage production 2

The red ginseng nano liquid obtained by the extract method was mixed with water and deep water according to the compositions of Examples 12 to 14 of Table 4 (using Mixer) to prepare a red ginseng beverage to which deep water was added.

<Example 18 to Example 20> Red ginseng beverage production 3

The red ginseng nano liquid obtained by the extract method was mixed (using Mixer) the composition band water and the deep water of Examples 12 to 14 of Table 4, that is, using the technical method of patent PCT / KR2006 / 001774, that is, a membrane contactor (oxygen pressure 3 bar, 2.5 g of liquid pressure to prepare a red ginseng beverage was added oxygen and deep water.

After preparing the red ginseng nano liquid by the above-described method, deep water, water, and oxygen were prepared in the same composition as in Examples 12 to 20, and then tested by age group.

Example 12 Example 13 Example 14 Example 15 Example 16 Example 17 Example 18 Example 19 Example 20 Red ginseng nano liquid (g)    One 4 8 One 4 8 One 4 8 Water (g) 99 98 97 98 94 89 98 94 89 Deep water (g) One 2 3 One 2 3 Oxygen (mg / l) 30 45 60 30 45 60

The tasting test results for each age group in Examples 13, 16, and 19 are shown in Tables 5 to 7 below.

Age group preferences (Example 13) Sensory test 20's 30 spaces 40 spaces 50 spaces 60 spaces incense ☆ ★ ★ ★ ★ flavor ★ ★ ☆ ★ ★ color ☆ ★ ☆ ★ ☆

Age group preferences (Example 16) Sensory test 20's 30 spaces 40 spaces 50 spaces 60 spaces incense ★ ★ ★ ★ ★ flavor ☆ ★ ● ★ ★ color ● ★ ★ ★ ☆

Age group preferences (Example 19) Sensory test 20's 30 spaces 40 spaces 50 spaces 60 spaces incense ☆ ★ ★ ★ ★ flavor ★ ★ ☆ ★ ★ color ☆ ★ ☆ ★ ☆

Satisfaction Survey on Red Ginseng Beverage

★ 90 ~ 100 Very satisfied

☆ 80 ~ 90 satisfaction

● 70-80 normal

○ 60 ~ 70 Poor

■ 50 ~ 60 complaints

□ 40 ~ 50 Very Complaint

Example 21 Measurement of Particle Size of Red Ginseng Nanopowder

The inventors measured the particle size of the red ginseng nanopowder prepared by the method of Example 1.

The method of measuring the size of the nano powder is made by dispersing the sample in distilled water and measuring the degree of dispersion by irradiating laser light. First, a sample was prepared by mixing 0.5 ml of the concentrated red ginseng liquid sample (before drying with red ginseng powder) in 3 ml of purified water. Another sample was prepared by mixing 3 ml of purified water with the fiber removed in the fifth step of the manufacturing process of Example 1.

As a result, data as shown in FIG. 2 was obtained. In summary, 534 nm is 52.3%, 141 nm is 34.4%, which corresponds to the red ginseng nanopowder of the present invention. The results of electron microscopy of each particle are shown in FIG. 3.

<Example 22> Lymphocyte proliferation ability test of red ginseng nano powder

The present inventors prepared the red ginseng nano powder prepared by the method of Example 1.

Splenocytes from 6 to 8 weeks old BALB / c mice were harvested and dispensed with 1.5 X 10 ⁵ cells per 96 well flat-bottom microplate wells containing different doses for 72 hours at 37 ° C and 5% incubator. Incubated. After treatment with ³ H-Thymidine at 2 μCi / well, the cells were further incubated for 4 hours, the cells were harvested, scintillation cocktails were added, and cpm was measured by β-counter to obtain stimulation index (SI). Concanavalin A (Con A) was used as a standard. The stimulus index was calculated by the following equation.

Stimulation index of = Corporation (cpm) in the samples incubated spleen cells ³ H-Thymidine in Corporation (cpm) / ³ H-Thymidine in the spleen cells incubated with control culture medium of

The results are shown in Table 8 below.

Lymphocyte proliferation test results division Lymphocyte proliferation (SI) Control PBS: 1.0 Positive control group Con A: 40.9 (2.5 μg / ml) Example 1 32.9 (500 μg / ml)

As shown in Table 8, the lymphocyte proliferation capacity of the control group was 1.0, but Example 1 of the present invention was 32.9 (500 µg / ml). Therefore, the red ginseng nanopowder of the present invention was found to be excellent lymphocyte proliferation ability.

<Example 23> Lymphokine-Activated killer (LAK) cell production test of red ginseng nano powder

Red ginseng nanopowder prepared by the method of Example 1 of the present invention was prepared. LAK cell production capacity was used for 4 hours 51Cr release method.

Working cells were diluted in spleen cells with RPMI640-5% FBS medium to 3.0 X 10 cells / mL in a 24-well plate (Corning, New York, USA), followed by adding 30 U / mL of IL-2 or sample. 2 ml of each was incubated for 5 days at 37 ℃, 5% CO₂ incubator. After 5 days, 2 ml of the culture was transferred to a 15 ml tube, centrifuged to precipitate the cells, and washed twice with HBSS. The concentration of cells was fixed at 5 x 10 cells / ml. Adjust the ratio of the prepared cells to the target cells of 100: 1, 30: 1 or 10: 1 and place them in a 96-well U-bottomed microplate (Corning, New York, USA) at 37 ° C and 5% CO₂. Incubated for 4 hours at. After 4 hours, centrifugation was performed at 2,200 rpm for 5 minutes, and 0.1 ml of the supernatant was measured, and cytotoxicity was calculated by measuring the intensity of γ-rays by Cr released into the supernatant with γ-counter.

After calculating cytotoxicity by the following equation, LU was obtained. Interleukin-2 (IL-2) was used as a standard.

Cytotoxicity (%) = ER-SR / MR-SR X 100

* ER: Experimental release count

SR: Spontaneous release count

MR: Maximum incorporation count

* LD10: means the number of effector cells capable of lysis of 10% target cells per million cells (higher value means more production of effector cells (LAK cell)).

The results are shown in Table 9 below.

LAK cell production test results division  LAK lytic units Control PBS: 1.0 Positive control group IL-2: 54.6 (30 units) Example 1 15.4 (100 μg / ml)

As shown in Table 9, the LAK cell production of the red ginseng nanopowder of the present invention confirmed that Example 1 is 15.4 LU10 (100 ㎍ / ㎖), it was found that the effect is excellent.

1 is a process diagram schematically showing a method for preparing red ginseng nanopowder using red ginseng (6 years old) of the present invention as a raw material,

Figure 2 is a spectral result showing the size distribution of the red ginseng nanopowder used in the present invention,

Figure 3 is an electron micrograph showing the size distribution of the red ginseng nanopowder used in the present invention,

4 shows a schematic system of an oxygen water production apparatus used in the present invention,

Figure 5 shows an example and the principle of implementation of the separator of the oxygen water production apparatus used in the present invention,

6 is a schematic view showing an embodiment of the adsorption method of the oxygen water production apparatus according to the present invention,

7 is a schematic view showing the principle of the contact membrane of the oxygen water production apparatus according to the present invention.

<Brief description of symbols used in the drawings>

1: contact membrane 2: oxygen generating means

3: two head compressor 4: oxygen tank

5: feed water bypass valve 6: water pressure measuring device

7: supply oxygen pressure gauge 8: compressed air pressure gauge

9: exhaust oxygen control valve 10: exhaust nitrogen control valve

11: oxygen water flow meter

12: dissolved oxygen measuring device

Claims (27)

Iii) selecting red ginseng as a raw material and drying after moist heat sterilization; Ii) powdering the dried red ginseng by performing first coarse milling and second milling milling; Iii) extracting fine red ginseng by adding water to the powdered red ginseng powder; Iii) removing fibrin from the red ginseng extract by centrifugation; Iii) removing monomolecular substances containing monovalent ions by reverse osmosis from the red ginseng solution from which the cellulose has been removed; And Iii) the red ginseng liquid from which the monomolecular substance has been removed is dried by a freeze dryer or a low temperature hot air dryer to produce a nano powder as a solid. Functional drink containing oxygen water and deep sea water. The functional beverage according to claim 1, wherein the size of the red ginseng nanopowder is 20 to 600 nm. delete The method of claim 1, wherein after the step of removing the fiber of step iii), the method further comprises the step of removing the extra fiber, which is a polymeric material, from the red ginseng from which the fiber has been removed by filtration using a separator. beverage. The functional beverage of claim 4, wherein the removing of the polymer material comprises a micro filtering system (MF) or an ultra filtering system (UF) having a size of 0.01 to 2 μm. The method of claim 1, wherein the removal of the monomolecular substance of step (iii) is to remove the low-molecular substances including pesticides using a nano-filtering system (NF), the monovalent ion rejection rate is 70 ~ 95% as a result Functional drink made with. The functional beverage of claim 1, further comprising concentrating the red ginseng solution by reverse osmosis in the red ginseng liquid from which the monomolecular material has been removed after the removing of the monomolecular material of step iii). The functional beverage according to claim 7, wherein the concentration uses a reverse osmosis system (RO), and as a result, the monovalent ion exclusion rate is 90 to 99.9%. 2. The functional beverage according to claim 1, wherein the red ginseng used as the raw material contains a ratio of red ginseng root to red ginseng 1: 1 to 0.2. The functional beverage according to claim 1, wherein the wet sterilization of step iii) is performed for 1 to 10 minutes at a steam temperature of 90 to 100 ° C. The functional beverage of claim 1, wherein the drying of the step iii) is hot air circulation drying. The functional beverage according to claim 1, wherein the dried red ginseng of step ii) has a water content of 5 to 25%. 2. The functional beverage of claim 1, wherein the powdering of step ii) has a final particle size of red ginseng of at least # 100. The method of claim 1, wherein the micronized red ginseng extract of step iii) is added to 4 g of water with respect to 1 g of red ginseng, and is performed 5 to 10 times at 50 to 86 ° C. for 5 to 15 hours. Functional drink to say. The functional beverage according to claim 1, wherein the removal of fiber in step (iii) is performed by removing fiber and dehydrating the fiber by using a centrifugal separator or a filter press. The method of claim 1, wherein the functional beverage is prepared by adding deep seawater taken from 200 m or less of water depth of 0.1 to 5 times the mass of red ginseng nanopowder and diluting it with 5 to 100 times purified water. Functional drink. 17. The functional beverage of claim 16, wherein said deep sea water contains concentrated deep water. 17. The functional beverage of claim 16, wherein the deep sea water of the East Sea contains desalted deep water. The functional beverage of claim 1, wherein the oxygen content of the oxygenated water is 12 to 150 mg per liter of the functional beverage. The method of claim 1, wherein the oxygen water Oxygen generating means for separating oxygen from the supplied compressed air; A contact membrane supplied with oxygen generated from the oxygen generating means and dissolved in water; And And a second head receiving air and supplying the oxygen to the oxygen generating means and applying a vacuum to the oxygen generating means to increase the purity of the separated generated oxygen and simultaneously supplying the oxygen to the contact membrane under pressure. Functional drink characterized in that it is produced from the oxygen water production apparatus having a two-head compressor. The functional beverage according to claim 20, wherein the oxygen generating means and the contact membrane of the oxygen water producing apparatus are porous membranes or nonporous membranes. 21. The functional beverage according to claim 20, wherein the oxygen generating means and the contact membrane of the oxygen water producing apparatus are hollow fiber type or flat membrane type. 21. The functional beverage according to claim 20, wherein the oxygen generating means of the oxygen water producing apparatus employs an adsorption method or a separation membrane method. 21. The method of claim 20, wherein the oxygen water production apparatus is supplied with air to the compressor and the oxygen generating means by applying a vacuum to the oxygen generating means to increase the purity of the separated oxygen generated at the same time to the contact membrane A functional beverage further comprising a vacuum pump for supplying under pressure. 21. The functional beverage according to claim 20, wherein the oxygen water production device further comprises an oxygen bottle for storing oxygen water obtained through the contact membrane. 26. The functional beverage of claim 25, wherein the oxygen water producing device includes at least one of an oxygen water flow meter and a dissolved oxygen measuring device on one side of the oxygen water tank. The method of claim 1, wherein the oxygen water is compressed air and supplied to the oxygen generating means; Supplying oxygen separated from said oxygen generating means to a contact film; And dissolving oxygen by supplying water to the contact membrane.

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