KR100854701B1 - Functional beverages composing ginseng steamed red concentrated solution - Google Patents

Abstract

A functional drink containing red ginseng concentrates, oxygen-rich water and deep sea water is provided to secure excellent dispersity, mixing property and absorption property, to be rich in inorganic material and useful as a functional health food, particularly used as a drink for preventing and improving immune-related disease. A functional drink contains red ginseng concentrates, 12 to 150mg oxygen-rich water per a functional drink and deep sea water. The red ginseng concentrates are obtained by the steps of; heating red ginseng at 100 to 120deg.C; mixing purified water of 4 to 8 times mass of red ginseng and extracting at 80 to 95deg.C by an indirect steam heating method; sterilizing at 90 to 100deg.C; cooling at 0 to 10deg.C and centrifuging at 5,000 to 8,000rpm to remove impurities; concentrating by a low temperature vacuum concentration method of 55 to 65deg.C; and sieving with a sieve of No 100 to 140. The extraction is performed 5 to 8 times in a continuous tank or 2 to 5 times in a batch type tank. The red ginseng contains red ginseng root and the root hair of red ginseng in a ratio of 1:1 to 0.2. Deep sea water pumped from a depth of less than 200m below a sea level is diluted with 5 to 100 times their weight of purified water.

Description

Functional Beverage Containing Red Ginseng Extract {Functional Beverages Composing Ginseng Steamed Red Concentrated Solution}

The present invention relates to a functional beverage containing red ginseng concentrate.

Korean ginseng is a medicinal herb that is toxic and unburned according to the ancient Chinese book of the rapid brewery (BC 33-48), Sanghanron (AD: 191-220), and Sinnonboncho (AD 483-496). Efficacy is described in the concept of 上 藥). Jeonchisam (田七 參) has been described as having a hemostatic effect on the herbaceous herb (本草綱目: AD 1598). The main active ingredient of ginseng is ginsenoside, and the chemical structure is elucidated and pharmacologically active ingredient is revealed through continuous research. The main characteristic of saponin, an active ingredient of ginseng, has a large lipolytic ability in the human body, promotes the absorption and digestion of nutrients, promotes metabolism by activating enzymes in cells, and promotes energy recovery by promoting serum protein and increasing energy. It is known to have an effect on improving anorexia, etc.

However, research on polysaccharides other than saponins is ongoing. Typically, ginseng polysaccharides, which are non-saponin-based substances, are complexes of polymer polysaccharides obtained from separation by the difference in molecular weight and ionic strength. These ginseng polysaccharides are known to have various effects such as hematopoietic promoting action, bone marrow defense action, cancer cell killing immune cell generation action, radiation sensitivity action, hypoglycemic action action.

Ginseng is divided into three types: fresh ginseng, red ginseng, and white ginseng, which maintain the original shape of ginseng. Because it contains moisture, it is prone to decay or damage during distribution, so long-term storage is difficult without special storage facilities or packaging.

Red ginseng is light yellow brown or light red ginseng that is dried by steaming without removing the reticulum of reticulum, which is a reticulum, which is also known as ginseng or white ginseng. It has been reported to have indirect effects on sexual behavior or reproductive effects, anti-inflammatory and anti-tumor effects, by acting on excitatory, hematopoietic and hypoglycemic, hepatoprotective, endocrine systems.

Meanwhile, the ginseng component contains active ingredients that inhibit cancer cell proliferation and formally induce normal cells. Anti-cancer activity and anti-cancer activity enhancing effect of anticancer agents have been suggested. It is reported that red ginseng contains an active ingredient that inhibits cancer cell metastasis and anticancer drug resistance. G-Rh2, a red ginseng ingredient, causes the so-called "Apoptosis" that kills cancer cells and interferon. It has been found to have an effect of promoting production. In addition, red ginseng extract has an effective effect on intracellular macrophage activation and antibody production.

The saponin component of red ginseng has the effect of promoting the relaxation of blood vessels, which plays an important role in the suppression of atherosclerosis and blood pressure control, and the protection of endothelial cells. Red ginseng has the effect of improving the red blood cell transformation ability to improve peripheral circulation. Red ginseng (saponin) has the effect of inhibiting the proliferation of vascular smooth muscle cells. Red ginseng has the effect of improving cholesterol metabolism (Korea-Japan Ginseng Symp, 1996).

In addition, red ginseng blood pressure lowering effect is associated with the action of promoting the vasorelaxation reaction of the red ginseng saponin component. Red ginseng improves quality of life (QOL) in patients with hypertension and is expected to be useful as adjuvant therapy in combination with hypertensives (M. Yamamoto, Ginseng Review. Vol 9, 1990).

Red ginseng has an ovarian function revival effect by improving microcirculation. Red ginseng is useful for improving female menopausal symptoms. Red ginseng (component) is useful for bone formation and improvement of bone biomechanical properties in experimental osteoporosis-induced animals ("Influence of Korean Red Ginseng Irradiation on the Physiological Functions of Elderly Female Rats", Korean Journal of Food and Nutrition, 2000 ). Red ginseng (saponin fraction) has the effect of inhibiting the development of stress ulcers and promoting the healing process of the symptoms of gastric ulcers caused.

Red ginseng contains ginseng as a raw material, and ginseng contains a large amount of saponin substance called ginsenoside, and each of these ginsenosides improves symptoms of central nervous system such as anxiety, insomnia, depression, and depression in addition to nourishing tonic. Efficacy in improving intellectual performance by improving cognition and memory, enhancing nonspecific bio-resistance against external harmful factors, normalizing living body against physical, chemical and biological external changes, and promoting blood glucose resistance and detoxification Ginseng because it has various pharmacological effects, such as protecting liver damage and promoting liver regeneration, enhancing cardiomyocyte protection and cardiovascular function, lowering blood cholesterol content, controlling blood pressure, and inhibiting cancer cell metastasis and resistance formation of anticancer drugs. Has been widely used as a herbal medicine since ancient times, but recently it has been used as a health food. This has been prepared is consumed.

Red ginseng (紅 蔘) is a herbal medicine that is processed by numerical method, which is a traditional pharmaceutical technique, and is manufactured by special steaming, drying and processing process of ginseng. During this process, the starch particles in ginseng tissue are gelatinized, the tissue is firm, and various enzymes are inactivated to stabilize the quality. In addition, a secondary ingredient change occurs during the temperature treatment process to produce a unique active ingredient (ginsenosides Rs, Rg1, Rg2, Rh1, etc.) that do not exist in ginseng or white ginseng, and active ingredients such as antioxidant activity. The content is increased (Kitagawa et al. 1983, 1992). However, the ginsenosides Rb1, Rb2, Rc, Rd, and Re, which are the main components of red ginseng, are unstable in the change of heat and extraction time when extracted for more than 10 minutes at high temperatures exceeding 100 ° C, especially diol ginsenosides. Its composition is known to change its form due to its weak heat resistance, which is easily converted into triol through hydrolysis reaction (Pak Myung-Han, Ph.D. ).

In addition, ginseng contains 60 ~ 70% of carbohydrates such as starch, but it is a specific ingredient that cannot be found in other plants. Ginseng saponin (ginsenoside), polyacetylene (polyacety lene), antioxidant active phenolic compound, and high liver function Superstitions (gomisin-N, -A), insulin-like acidic peptides are included. The pharmacological effects of these ingredients are antidiabetic, anticancer, arteriosclerosis and hypertension, liver protection, gastrointestinal disease, hangover removal, anti-aging, anti-fatigue and antistress, brain activity, blood Circulation, immune function enhancement, anemia treatment, radioactivity defense, AIDS virus proliferation inhibition.

On the other hand, as the public's demand for health has recently increased, there is a growing interest in water purifiers that can produce functional water, such as oxygen water, beyond simple water purification functions. In order to satisfy the demands of consumers, products in which oxygen of saturated concentration is dissolved in water or liquor are sold. Also, there are products in which oxygen water is added by adding oxygen to water purifier and cold and hot water. Conventional methods for producing oxygen water include a blower method that increases oxygen and dissolution rate by providing oxygen using an oxygen generator or a high-pressure gas container to produce oxygen and making it into microbubbles to increase dissolution rate. It is known to pressurize to increase the pressure, the dispersion method using the ejector principle. However, the pressurization method can increase the dissolution efficiency in the supersaturation concept, but there is a difficulty in applying a high pressure. The blower method and the ejector method are lost by rising upward through the bubble before oxygen is dissolved in water. There was a problem in that the efficiency is insufficient to produce supersaturated dissolved oxygen water.

In addition, the oxygen water purifier for producing oxygen water by such a conventional method for producing oxygen water requires a complicated device, which increases the size and weight of the water purifier itself, which is cumbersome to handle and install. In addition, the conventional oxygen water purifier must be equipped with a separate sterilizer or a separate filter to prevent secondary contamination by bacteria, the method of using a UV sterilizer in the conventional water purifier is bulky, expensive It is complicated to install, there is a risk of failure, and has a problem such as a long time required for sterilization is questioned the practical availability.

Common drinking water for human consumption includes tap water, purified water, natural water, boiled water, and distilled water. Recently, as the social atmosphere that puts importance on health has matured, many attempts to find clean and healthy water have been increasing, and accordingly, preference for purified water and natural water rather than tap water is increasing. However, the purified water has a problem that the beneficial minerals, etc., which are beneficial to the body are removed while passing through the filter, and the amount of dissolved oxygen is sharply lowered, and even in the case of natural water, its consumption is not much compared to the preference due to the distrust of environmental pollution. to be. Distilled water or boiled water is not able to supply oxygen to the human body in particular because all dissolved oxygen is not evaporated.

Dissolved oxygen present in the water can increase the blood oxygen concentration of the human body, such an improvement in blood oxygen concentration helps to make the human body healthier by performing the function of activating the human metabolism. In particular, insufficiency of the respiratory organs, respiratory failure of the skin, and oxygen deficiency due to excessive use of oxygen are known to cause cell insufficiency and cause various diseases or even death.

Therefore, various attempts have been made to increase the amount of dissolved oxygen present in the water. A conventional technique for preparing such oxygen water is a blower method that increases the dissolution rate by increasing the gas-liquid contact area by using an acid method using microbubbles. And a pressurization method for increasing pressure, a dispersion method using an ejector principle, and the like are known. Among them, the pressurization method can increase the dissolution efficiency in the supersaturation concept, but it is difficult for the general person to operate by applying a high pressure, and the blower method and the ejector method are floated upward through bubbles before oxygen is dissolved in water. Because of the loss, the oxygen dissolution efficiency is lowered, and thus there is an inadequate aspect for producing a high concentration of supersaturated dissolved oxygen water.

In particular, in the case of an adsorption method in which oxygen generated using an adsorbent is dissolved in water, since adsorbent dust is generated due to the use of the adsorbent, adsorbent dust harmful to the human body tends to be dissolved in water. There is a problem in that it is economically undesirable because a complicated configuration such as having various filters or tanks is required.

Therefore, there has been a demand for an oxygen water production apparatus capable of suppressing contamination while dissolving oxygen at high efficiency and high concentration, and a method for producing oxygen water capable of dissolving high concentrations of oxygen only by a simple process.

On the other hand, the deep sea water and surface sea water of 200 m or less have similar concentrations of salinity (NaCl) and most minerals, but nutrients (nitrogen nitrate, phosphoric acid, silicon), viable count, and water temperature have significant differences. The water temperature of deep sea water is almost constant throughout the year, and the surface water temperature of sea level is 16 ~ 28 ℃, but the water temperature of 374m deep water is 9 ℃, which shows low temperature stability, so it is low in plankton, microorganisms, especially pathogenic bacteria. There is (淸 淨 性). Deep seawater was not detected in the test of 10 kinds of bacteria including pathogenic Escherichia coli and pathogenic virus. The total viable count is clean water from 1/10 to 100% of surface water.

In addition, deep sea water contains about 5 to 10 times more inorganic nutrients than surface water and contains more than 70 different minerals, which can be harmful if consumed in large quantities. Deep mineral water, which contains only a small amount, has a deep relationship to human health, such as copper and zinc, which are essential trace elements. In addition, deep sea water is matured for a long time in the low temperature and high pressure of the deep sea, and the organic content is low and pH is lower than the surface water (around pH 7.8), and it is a group of water molecules compared to the land or mineral water of the land. ) Has a small characteristic.

Beverages are made by adding various additives, such as sweeteners, to natural, sprinkled or purified water. Beverage products include soft drinks, fruit drinks, sports drinks, juices, and the like, and various beverages are made according to the types and additives of the additives. However, mineral drinks that use natural water (mineral water), natural water or purified water that have spun underground water, or add specific ingredients may supply minerals of specific ingredients, but cannot supply various minerals necessary for the human body. In addition, the existing beverages can be ingested certain components as a functional beverage for health, but by using the natural water, natural water or purified water sprinkled underground has not been able to function as a source of various minerals.

Deep sea water is originally aged for a very long time, which is rich in mineral nutrients necessary for supporting living organisms, is organic in nature and bacteria, and is clear, stable at low temperatures. Deep water generation shows that seawater in Greenland, Antarctica, and Vladivostok rapidly cools when it meets the glacier, and the cold, heavy seawater, which mixes with the salt that freezes out, does not go deeper into the ocean. Heavy streams descend from the depths of 200 m up to 4,000 m deep into the sea to form thick bands. Deep water that flows through deep seas does not mix with surface waters, like water and oil, due to temperature differences of more than 20 degrees. In the deep sea, where there is no light, only decomposing instead of photosynthesis, the deep water is rich in organic nutrients with various minerals. Table 1 compares the characteristics of deep ocean water at each intake area. Deep sea water consists of sources of origin and indigenous water produced at the intake area, and the composition of the intake area is slightly different. Therefore, each marine deep water intake zone is developing its own product. The source of deep water in the East Sea of Korea is the seawater of Vladivostok. The sedimented seawater of Vladivostok and the high water of the East Coast are combined to form the deep sea water inherent in the East Sea of Korea. It is distributed throughout the east coast such as Goseong, Yangyang, Gangneung, Uljin, and Ulleungdo, and has a slightly different characteristic from that of other deep sea waters in Japan and the United States. As shown in Table 1, the characteristics of deep sea water (goseong) in Korea are higher in water temperature than in Kochi, Okinawa, and Hawaii in the United States, and the contents of nutrients such as phosphoric acid and silicic acid are similar to those of Kochi and Toyama. More than that. In addition, dissolved oxygen was found to be higher than in other regions.

division Water temperature salt pH Nutrient Dissolved oxygen (mg / ml) phosphate nitrate Silicate East Sea Deep Water (Goseong) 1.52-1.9 33.72-33.94 7.19-7.90 1.7 ~ 4.3 3.6-13.3 72.0-108.0 9.13-9.47 Kochi Prefecture Deep Sea Water 8.1 ~ 9.8 34.3 ~ 34.4 7.8 ~ 7.9 1.1-2.0 12.1 ~ 26.0 33.9 ~ 56.8 4.1 ~ 4.8 Deep water in Toyama, Japan 1 ~ 2 34.1 7.74 1.2 ~ 1.8 14-26 30-38 5.0 ~ 6.0 Japan's Okinawa Prefecture Deep Water 9.0 34.71 7.93 3.05 21.89 44.81 US Deep Water in Hawaii 8.2-10.7 34.37-34.29 7.45-7.74 2.89-3.15 39.03-40.86 74.56 ~ 79.2 1.24-1.45

Accordingly, the present inventors conducted research to broaden the application field of red ginseng powder, and developed a high-density red ginseng concentrate composed of solids of various particle sizes while maintaining the natural ingredients of red ginseng, and the Japanese Kochi Prefecture, Okinawa Prefecture, By developing Korea's own East Sea deep sea water, which contains more minerals and dissolved oxygen than deep sea water in Hawaii, USA, and the functional beverage in which the inventors dissolved the oxygen water using the oxygen water production apparatus of Patent No. 678489, which the inventors previously invented. The present invention has been completed.

An object of the present invention is to provide a functional beverage in which red ginseng concentrate is dissolved in deep sea water and natural oxygen-rich oxygen water.

In order to achieve the above object, the present invention provides a functional beverage containing red ginseng concentrate, oxygen water containing a large amount of oxygen and deep sea water.

Hereinafter, the present invention will be described in detail.

In the functional beverage of the present invention, the red ginseng concentrate iii) screening and heat treating red ginseng as a raw material; Ii) mixing purified water with the selected red ginseng and extracting at high temperature; Iii) heating and sterilizing the extracted red ginseng solution; Iii) cooling the sterilized red ginseng extract and separating and removing impurities; Iii) concentrating and aging the red ginseng extract from which the impurities are removed; And iii) measuring the sugar and starch content of the red ginseng extract, and the screening and heat treatment of step iii) include three ginseng, red ginseng, drying, shaping, selection, and a heat treatment of 100-120 ° C. More preferably, the purified water of step ii) is more preferably 4 to 8 times the mass of raw red ginseng, the high temperature of step ii) is more preferably 80 to 95 ℃ by steam indirect heating method, the ii Extraction of the step) is preferably performed 5 to 8 times in a continuous tank or 2 to 5 times in a batch tank, and the heating and sterilization of step iii) may be performed at 90 to 100 ° C. by heating and instant cooling / It is more preferable to sterilize by heating method, the cooling of the step iii) is more preferably carried out at 0 ~ 10 ℃, the removal of impurities in the step iii) using a centrifuge of 5,000 ~ 8,000 rpm It is more preferable to remove the starch and foreign debris cooled by cooling at a flow rate of 10 ~ 30 ℓ / min, the concentration and aging of the step iii) by concentrating by low temperature vacuum concentration method of 55 ~ 65 ℃ # 100 ~ 140 After removing the impurities from the sieve, it is more preferably aged for 10 hours or more, and the sugar and starch content of step iii) is more preferably 40-75 brix sugar content and 2-3% starch content. It is more preferable to perform chromaticity, water content and microbial analysis of the red ginseng concentrate after step), wherein the chromaticity is 0.20 to 0.50, the water content is 30 to 45%, and the microorganism is 100 / g or less. .

In addition, the oxygen content of the oxygen water in the functional beverage of the present invention is preferably 12 to 150 mg per liter of functional beverage.

In addition, in the functional beverage of the present invention, the oxygen water is oxygen generating means for separating oxygen from the supplied compressed air; A contact membrane supplied with oxygen generated from the oxygen generating means and dissolved in water; And a first head receiving air and supplying it to the oxygen generating means; and a second head applying a vacuum to the oxygen generating means to increase the purity of the separated generated oxygen and simultaneously supplying it to the contact membrane under pressure. It is preferable that the oxygen generating means and the contact membrane of the oxygen water producing apparatus having a; two-head compressor comprising a; and the oxygen generating means and the contact membrane of the oxygen water producing apparatus is more preferably a porous membrane or a non-porous membrane, the oxygen water producing apparatus More preferably, the oxygen generating means and the contact membrane are hollow fiber type or flat membrane type, and the oxygen generating means of the oxygen water producing apparatus more preferably employs an oxygen cylinder, an adsorption method or a separation membrane method. It is supplied to the oxygen generating means and supplied to the compressor and the oxygen generating means by applying a vacuum to the purity of the separated oxygen generated It is more preferable to further include a vacuum pump which increases and simultaneously supplies it to the contact membrane under pressure, and more preferably, the oxygen water production apparatus further includes an oxygen bottle for storing oxygen water obtained through the contact membrane. At this time, the oxygen water production apparatus is more preferably provided with at least one of the oxygen water flow rate meter and the dissolved oxygen measuring device on one side of the oxygen can.

In addition, in the functional beverage of the present invention, the oxygen water is compressed air and supplied to the oxygen generating means; Supplying oxygen separated from said oxygen generating means to a contact membrane; And dissolving oxygen by supplying water to the contact film.

In addition, in the functional beverage of the present invention, the functional beverage is preferably prepared by adding deep sea water taken from 200 m or less of water depth of 0.1 to 5 times the mass of red ginseng concentrate, diluted with 5 to 100 times purified water. It is more preferable that the deep sea water of the East Sea contains the concentrated deep water or the desalted deep water.

The functional beverage according to the present invention refers to a beverage in which deep seawater of deep seawater is added, a beverage in which deep seawater of concentrated east sea is added, a beverage in which deep seawater of desalted water is added, or a beverage in which mineral part is added to deep seawater of desalted water, etc. For example, soft drinks, fruit drinks, sports drinks, juices, etc. are mentioned.

The functional beverage of the present invention is prepared from red ginseng concentrate, oxygen water containing oxygen, and deep sea water. Thus, the manufacturing process of the red ginseng concentrate, the manufacturing process of oxygen water, and the treatment of deep water will be described separately.

<Production of Red Ginseng Concentrate>

1. First Process: Red Ginseng Screening and Heat Treatment

After inspecting the freshly grown ginseng contracts, they purchase only those that have passed and processed them through the three-ssam / red ginseng / dry / formation / selection process, and release the six-year-old red ginseng stored in the raw material warehouse.The ratio of red ginseng root and red ginseng is 7: 3 It is precisely weighed and uniformly heat treated (pretreatment) at about 110 ° C.

2. Second Process: Extraction

Mix 5 ~ 6 times purified water of red ginseng and extract it at 80 ~ 95 ℃. At this time, the extraction is repeated 6 ~ 7 times in the continuous (red ginseng tablet process) tank, 3 to 4 times in the batch (red ginseng tablet process) tank and transported to the heating tank. The difference between the continuous process and the batch formula here is that the continuous formula is advantageous for extracting the surface components of red ginseng and extracting the color / flavor uniformly with high concentration, and the batch formula extracts a large amount in a short time while maintaining the unique components and aromas. It is advantageous.

3. Third process: heating and sterilization

 The red ginseng extract is heated at 95 ° C. and sterilized by an instant cooling / heating method.

4. The fourth step: remove impurities

The sterilized red ginseng extract was cooled (4-7 ° C.) to facilitate centrifugation, proceeded to a specified flow rate (20 L / min) in a centrifuge at about 7,500 rpm, and impurities such as cooled starch and other foreign debris. Remove it.

5. The fifth step: concentration and ripening

The red ginseng extract from which the impurities were removed was concentrated by a low temperature (55-65 ° C.) vacuum concentration method to remove impurities from the 120 # sieve, and then aged in a 600L thermal tank for 24 hours.

6. The sixth step: measurement of sugar and starch content

Viscosity and starch content are measured by measuring the sugar content. Sugar concentrations range from 40-75 Brix and starch should range from 2 to 3% to be considered a suitable red ginseng concentrate. In addition, the final product inspection method of the red ginseng concentrate is analyzed for color, moisture content, microorganisms and the like. The chromaticity is within 0.20-0.50, the water content is within the range of 30-45%, and the microorganism is less than 100 / g is suitable. As a result, in the following examples of the present invention, the solid content was found to be 64% and the moisture content was 36%.

In order to test the biological immune enhancing activity of the red acid concentrate of the present invention, lymphocyte proliferation ability, LAK cell production (tumor cell killing activity) and nitrogen oxide generation ability were tested. As a result, the lymphocyte proliferation capacity of the high concentration of red ginseng of the present invention was found to be 22.4 (500 ㎍ / ㎖) compared to the control 1.0 showed that the effect is significantly higher. In addition, LAK cell production (tumor cell killing activity) for the high concentration of red ginseng of the present invention was 9.7 LU10 (100 ㎍ / ㎖) compared to the control, it was found that the effect is excellent.

<Production of Oxygen Water>

As the oxygen water of the present invention, the oxygen water producing apparatus and the method described in the registered patent No. 678489 was used.

The apparatus for producing oxygen water used in the present invention may additionally antibacterial treatment of the oxygen generating means and the contact membrane, which may be achieved by installing an ultraviolet lamp or an antibacterial sponge at the front or the rear of the feed water.

In addition, the method for producing oxygen water is compressed air and supplied to the oxygen generating means; Supplying oxygen separated from said oxygen generating means to a contact film; And dissolving oxygen by supplying water to the contact film.

Hereinafter, with reference to the accompanying drawings will be described in more detail the oxygen water production apparatus of the present invention.

Figure 6 is a schematic system of the oxygen water production apparatus used in the present invention.

The oxygen water producing apparatus is basically provided with oxygen generating means (2) for generating oxygen from the supplied air and a contact film (1) for dissolving high concentrations of oxygen supplied from the oxygen generating means in contact with water to dissolve it. .

As the oxygen generating means 2, conventionally known oxygen generating means can be used without limitation, and for example, an adsorption method or a separation membrane method can be used. In the case of the adsorption method, dust is inevitably generated due to the use of an adsorbent, but in the present invention, a separate means for removing the adsorbent is required. In (2), the dust can be naturally filtered, so that a separate dust removal device is not required, and thus the adsorption method using an adsorbent can be freely used without any limitation.

As shown in FIG. 6, the basic principle of the adsorption method is to first adsorb nitrogen, which is a high selectivity component, while compressed air passes through an adsorption tower filled with an adsorbent such as a molecular sieve under high pressure, and oxygen of low selectivity is first adsorbed. Passes through and is discharged out of the adsorption tower. The compressed air through the compressor can be cooled as needed to increase the adsorption power or to remove moisture. The cooled air is introduced into one of the two adsorption towers, and the nitrogen molecules in the air are different from the oxygen molecules due to the difference in adsorption power. First adsorbed. When the adsorbent in the adsorption tower is saturated by the nitrogen molecules, the solenoid valve is operated by the electronic control device that senses this, and the compressed air is transferred to another adsorption tower. At the same time, the adsorption tower saturated with nitrogen gas is desorbed by purging for regeneration. By performing the discharge is to be discharged through the nitrogen control valve (10). This process can be repeated to continuously produce oxygen.

This repetition process is performed every few tens of seconds, and the adsorbent is repeatedly adsorbed and desorbed.

As the oxygen generating means 2, a separation membrane method can be used in addition to the adsorption method. Such a separator may be selected from a plate type and a hollow fiber type as a porous or nonporous gas separator.

As an example of the basic principle of the separation membrane method, it is due to the difference in the permeability of each gas component in various gas mixtures permeating through the membrane. As shown in FIG. 7, the compressed air supplied to the separator is composed of various gas mixtures including oxygen and nitrogen, and the gas mixture is in contact with one side of the separator to selectively permeate at least one of the gas components. .

Gas components selectively permeated by the membrane must pass faster than at least one gas component of the gas mixture.

Relatively impermeable gas components must penetrate the membrane more slowly than one or more components of the gas mixture. By this principle, the gas mixture is separated into two streams, which are selectively permeate gas streams and those which are not permeate gas streams. Therefore, in order to properly separate the gas mixture, a film forming material having a high permeability for a specific gas component should be selected.

In order to selectively separate and concentrate the mixed gas, the gas separation membrane generally has an asymmetric structure composed of a dense separation layer on the surface and a porous support on the bottom. The selective separation ability of the mixed gas for a particular membrane forming material depends on the degree of compaction of the separation layer, and the flow rate of the selectively separated gas depends on the thickness of the separation layer and the degree of porosity of the porous support, the substructure of the asymmetric membrane. . In order to selectively separate the mixed gas, the surface of the separation layer should be as free of defects as possible, and the pore size should be 5 mm or less. In addition, the separation layer should be as thin as possible to obtain high gas permeability because the gas permeability is inversely proportional to the effective membrane thickness. In order to minimize resistance to gas flow selectively passing through the separation layer, the asymmetric membrane substructure should preferably have a porous structure. As the form, it can manufacture into a hollow fiber type, a flat membrane type, etc.

Oxygen generated by the oxygen generating means 2 such as the separation membrane or the adsorbent is moved to the contact membrane 1 to be dissolved by contact with water. The other structure of the contact membrane 1 can pass the liquid to the outside or inside of the tube, at which time it is dissolved by supplying concentrated oxygen from the opposite side. Oxygen generated from the oxygen generating means 2 is introduced through the contact membrane 1, and no bubbles are generated at this time, which is caused by diffusion of oxygen passing through the contact membrane 1 into a liquid such as water. It means to dissolve. In the present invention, by using such a contact membrane (1) it is possible to simply dissolve the oxygen of the molecular unit in water to increase the content of dissolved oxygen and also increase the dissolution time. In particular, there is an advantage that the installation of the injector, which is used in the prior art, the pressure of the vessel, the temperature of the vessel is not required.

In the case of the contact membrane 1, it is more preferable to allow the flow of liquid to the inside of the tube in consideration of the influence of the contact area and the flow rate, etc., and if the length of the contact membrane 1 is long, the efficiency tends to decrease. It is preferable not to form too long. It is also preferable that the contact film 1 be formed in a corrugated structure in order to increase the surface area in contact with the liquid. In addition, both porous and non-porous are possible, and in order to induce gas diffusion more appropriately, a non-porous membrane is preferable. As the form, it can manufacture into a hollow sand shape, a flat film shape, etc. As the material, a film made of polysulfone, polysulfone composite, polypropylene, polyethylene, polyethersulfone, polyimide, teflon, silicone, polytetrafluoroethylene and mixtures of two or more thereof can be used.

A liquid such as water flows into the contact membrane 1 through one end thereof. In this case, a water pressure measuring device 6 is mounted on a part of the pipe to measure the water pressure. By discharging the inflow water through 5) it is possible to ensure that the appropriate water pressure always flows into the contact membrane (1).

A separate compressor and a vacuum pump may be used to supply compressed air to the oxygen generating means 2. That is, a compressor for compressing and supplying air to the oxygen generating means 2, and a vacuum pump for inducing high-purity oxygen generation by applying a vacuum to the oxygen generated from the oxygen generating means and supplying it to the contact film 1 It may be provided separately.

As another method, the present invention provides a first head for supplying compressed air obtained by compressing air and compresses it, and vacuums oxygen generated from the oxygen generating means to generate higher purity oxygen and at the same time, the contact membrane It is more preferable to use a two head compressor 3 having a second head which is supplied under pressure in comparison with using them separately. Because such a two-head compressor (3) can use the pressurization and vacuum at the same time, even in low pressure operation to induce a pressure difference with one compressor to the maximum, compressed air and / or to the oxygen generating means (2) and the contact membrane (1) By allowing the concentrated oxygen supply at the same time, it is possible to generate a high concentration of oxygen from the oxygen generating means 2, which passes through the contact membrane 1 under pressure, which is less than the concentrated oxygen generated in the oxygen generating means 2. It generates oxygen with a purity of about 10% higher, which allows this high purity oxygen to be dissolved in water in the form of a single molecule. In addition, the use of only one compressor can reduce the amount of power used, it is preferable to reduce the amount of noise generated during operation.

A pressure gauge 8 is attached to the output of the first head of the two-head compressor 3 to measure the pressure of the compressed air supplied to the oxygen generating means 2 to maintain an appropriate pressure. A pressure gauge 7 is also attached to the output unit, so that the appropriate pressure can be induced by measuring the pressure of concentrated oxygen generated from the oxygen generating means 2.

The apparatus for producing oxygen water according to the present invention may further include an oxygen bottle 4, which may serve to store oxygen water discharged from the contact membrane 1. The oxygen can 4 is also provided with an oxygen water flow meter 11 and / or dissolved oxygen measuring device 12 to control the operation of the oxygen water generating system. That is, when the oxygen water supply is excessive, the oxygen water flow rate measuring unit 11 detects this and stops the oxygen water generation system. In particular, by measuring the content of dissolved oxygen by the dissolved oxygen measuring device 12, it is possible to appropriately control the system by the automatic control device to have an appropriate oxygen content according to the intended use.

As the dissolved oxygen measuring apparatus 12, an oxygen sensor generally used can be used without any limitation, and there is, for example, a galvanic type. The galvanic type is an electrochemical oxygen sensor, in which a reduction reaction occurs at a noble metal electrode and a reduction current is generated according to the contacted oxygen concentration, and is a representative oxygen sensor that can be used within a temperature range of room temperature. It is widely used for low oxygen analysis.

Such an oxygen bottle 4 can be more usefully applied especially to a device such as a water purifier. That is, after storing the oxygen water in the oxygen can (4), it is convenient because it can be provided by cooling or warming through an appropriate method. Since the oxygen water is mostly used for human consumption, it is preferable to purify the water as much as possible, and it is preferable to purify the supply water before supplying the oxygen water production apparatus, that is, the contact membrane 1. In the case of purifying oxygen water after preparation, dissolved oxygen may be removed by various filters, which is not preferable.

It is preferable to perform an antibacterial treatment on the manufacturing apparatus so that bacteria do not propagate in oxygen water, and the photocatalyst, for example, may be used for the oxygen generating means 2, the contact membrane 1, the housing, the oxygen can 4, and the piping. Treatment with TiO ₂ , WO ₃ , ZnO, SiC, CdS, etc., or treatment with silver nanoparticles to impart antimicrobial properties, can be antimicrobial treatment through UV sterilization or antibacterial sponge during the supply of the feed water. Alternatively, the antimicrobial treatment may be performed by irradiating an ultraviolet lamp to the oxygen bottle itself. Such antibacterial treatment further increases the safety and stability of the oxygen water.

The oxygen water production process will be described in more detail according to the process sequence as follows.

First, the air compressed through the first head of the two-head compressor 3 is supplied to an oxygen generating means 2, for example, an adsorption tower or a separator, to generate a high concentration of oxygen. For example, nitrogen is discharged to the outside through the control valve (10). The high concentration oxygen separated by the oxygen generating means 2 is formed into oxygen of higher purity by vacuum through the second head of the two-head compressor 3 and is introduced into one end of the contact film 1 under pressure. . The injected high concentration of concentrated oxygen is dissolved in contact with the liquid such as water introduced to the other end of the contact film 1 by diffusion on the contact film. Oxygen water dissolved in a high concentration of oxygen is introduced into the oxygen can (4), the circulation process as described above is properly controlled by the oxygen water flow rate measuring instrument 11 attached to one side of the oxygen can (4). In addition, the content of the oxygen can also be appropriately controlled by the dissolved oxygen measuring device 12 attached to one side of the oxygen can (4).

Oxygen water generated by such a process can be used in water purifiers and the like, it can be used as a functional beverage such as the present invention. In addition, it can be used as a raw material of alcoholic beverages such as alcohol, ice creams such as ice cream, beverages and the like. In particular, high-quality oxygen can be dissolved in the water at a high concentration, thereby providing the user with a more useful healthy oxygen water capable of supplying oxygen to the body smoothly.

<Treatment of East Sea Deep Water>

Fill the container with deep sea water and place it in a sterilization chamber to sterilize the filling. At this time, the sterilized deep sea water is 10% or less in the beverage, the preferred range is 5% or less, the optimum range is 3%. It is preferable to mix other sweeteners and other additives with deep sea water in advance.

Alternatively, the deep sea water concentration is concentrated by reverse osmosis, electrodialysis or evaporation. As the concentrated deep sea water, the range of the beverage is 5% or less, and the preferred range is 3% or less.

Alternatively, deep sea water can be desalted. At this time, desalination is carried out by at least one treatment method from an electrodialysis method, a reverse osmosis membrane method, an ion exchange membrane method and a distillation method. At this time, the beverage adoption range of the deep sea deep water is 1-99%.

Alternatively, the deep sea water of East Sea can be evaporated by heat evaporation, vacuum evaporation, sun drying, ventilation drying, etc., and the evaporated dry matter is mixed with natural water, natural water or distilled water pumped underground, and filled into the container and sterilized. Place in a seal and sterilize the filling. Here, the range of employment for the beverage of evaporated dry water of deep sea water of East Sea is 2% or less, and preferably 0.05-0.5%. If it is 2% or more, too much salt is contained and it is not suitable as a drink.

Alternatively, sterilize the filling by filling the container with juice and concentrated deep sea water. When diluting the juice or the concentrated East Sea deep water with natural water, it is preferable that the salt concentration of the juice be within 5% depending on the amount of natural water used.

Another method is to sterilize the filling by filling desalted deep sea water, sweeteners or other additives into the container. The range where the desalination copper deep water can be employed is 1 to 99%, the preferred range is 5 to 50%, and the optimum range is 10 to 30%.

Another method is to concentrate the desalted deep sea water, fill the container with concentrated deep sea water and sweeteners or other additives and sterilize the filling. The range where the desalination-concentrated East Sea deep water can be employed is 1 to 99%, the preferred range is 5 to 50%, and the optimum range is 10 to 30%.

In another method, the desalted deep sea water, mineral salts, and other components other than the minerals are filled into a container, and the filling is sterilized.

The red ginseng concentrate of the present invention has a high density, good dispersibility, good mixing and absorbency, and another one component of oxygen water is a contact membrane, which does not cause dust, and the oxygen dissolution rate and dissolution time are improved. Oxygen water, another one-component deep sea water, is rich in low temperature stability and inorganic nutrients such as nitrogen, phosphorus and silicic acid, and is not contaminated by general bacteria or chemicals such as Escherichia coli, and has a stable aging and essential properties. Since the trace elements and various minerals are contained in a balanced manner, the beverage of the present invention containing such red ginseng concentrate and deep sea water is useful as a functional health food that prevents and maintains health from various diseases and diseases, and particularly for the prevention of immune-related diseases. And beverages for improvement.

Hereinafter, the present invention will be described in more detail based on the following examples. However, the following examples are only for illustrating the present invention, and the present invention is not limited to the following examples and may be changed to other embodiments equivalent to substitutions and equivalents without departing from the technical spirit of the present invention. Will be apparent to those of ordinary skill in the art.

Example 1 Preparation of Red Ginseng Concentrate

The present inventors prepared red ginseng concentrate (red ginseng tablet EXT) using red ginseng (6 years old) as a raw material (Fig. 1). First, the combination ratio of red ginseng root and red rice ginseng of red ginseng 6 years old was cleanly pre-treated (selected / heated) to 7: 3, and subdivided precisely for each extraction tank by 250-450 kg of extraction amount in a separate compartment. Then, the red ginseng raw material was added to an extraction tank, and 5-6 times purified water was mixed to maintain the extraction tank temperature at 80 ° C to 95 ° C by steam indirect heating, and extracted for 7-9 hours. The extraction was repeated. At this time, the extraction was carried out 6-7 times in a continuous tank and 3-4 times in a batch tank.

After the extraction was completed, the extract was mixed and then heated and sterilized. Then, the mixture was cooled to 4-6 ° C. in a cooling tank, and the cooled extract was passed through a 8,000 rpm centrifuge for 10-20 minutes to separate and remove impurities (starch, dregs, etc.).

Then, the pure red ginseng extract was concentrated and matured under reduced pressure to be suitable for manufacturing red ginseng tablets or red ginseng products.

Thereafter, the sugar content gauge for the semi-finished red ginseng concentrate (red ginseng tablets) was used to make 70-75 briquettes. The starch content was dissolved in alcohol and then precipitated and dried to calibrate the content to be within 2-3%. The unit was packed or set packed by bottling / tightening / camping.

Finally, the final inspection of the finished product was carried out to check whether the chromaticity is within 0.20-0.50, the moisture content is within the range of 30-45%, and the microorganisms are 100 / g or less and sanitized in the air warehouse after receiving approval for shipment.

The red ginseng concentrate prepared by the above method was found to have 64% solids and 36% moisture.

Example 2 Preparation of Oxygen Water

An oxygen water production apparatus as shown in Figure 8 was prepared. First, 1 kg / cm 2 of compressed air is injected into the oxygen generating membrane 2 (non-porous polysulfone composite membrane, diameter 48 mm * length 182 mm, effective membrane area 0.58 m 2) through the first head of the two-head compressor 3. The concentrated oxygen having the oxygen purity of about 42% was drawn through the second head, which is the other head, to the vacuum of 400 mmHg. It was added to the porous polysulfone composite membrane, diameter 70 mm * length 232 mm, effective membrane area 1.58 m 2). At this time, when the water pressure applied to the contact membrane was 1.5 kg / cm 2 or more, the flow rate was adjusted using a bypass valve.

The dissolved oxygen amount was measured by a dissolved oxygen measuring instrument (manufactured by Maxtec) at an experiment temperature of 25 ° C. and a concentrated oxygen supply pressure of 1 kg / cm 2, and 34 mg / l was obtained. The change according to the pressure at 25 ° C. was shown in Table 2 below. The change in dissolved oxygen with time is shown in Table 3 below.

Pressure (㎏ / ㎠) 0.2 0.4 0.6 0.8 1.0 1.2 1.4 Dissolved Oxygen Amount (mg / l) 14 19 24 29 34 38 41

Minutes 5 10 15 20 25 30 Dissolved Oxygen Amount (mg / l) 34.0 33.0 32.7 32.5 32.4 32.4

Oxygen water production apparatus according to the present invention as can be seen from the results of Table 2 and Table 3 is dissolved oxygen in the water in the water due to the use of the contact membrane simply because the dissolved rate increase and the dissolution time can be increased oxygen The number can be produced more efficiently. In addition, in order to generate oxygen in general, a pressure of 2 kg / cm 2 or more should be applied. However, in the present invention, a pressurization and vacuum of about 1 kg / cm 2 may be simultaneously used using a two-head compressor, so that a single compressor may be used even in a low pressure operation. By inducing pressure difference as much as possible, it is possible to simultaneously supply oxygen and concentrated oxygen to the contact membrane, thereby reducing power consumption and noise during operation.

In particular, in the case of general tap water, the dissolved oxygen amount is only 6 to 10 mg / l at room temperature, and the conventional method takes a method such as installing an injector, strengthening the pressure of the vessel, and lowering the temperature of the vessel to increase the oxygen dissolution rate. Dissolved oxygen amount can be supplied 14 to 41 mg / L, or more oxygen water without a separate oxygen dissolved device. As a result, when using the oxygen water production apparatus according to the invention it is possible to increase the dissolved time as well as increase the oxygen dissolution rate when applied to the existing additional equipment.

In addition, the oxygen dissolution rate can be further increased when the water temperature is reduced, and the area of the separation membrane and the contact membrane can be increased according to the capacity to provide an oxygen water supply device that is easy for the user to use.

In addition, due to the characteristics of the gas separation membrane, when the supply side pressure is increased to 1 kg / cm 2 or more, or the vacuum degree is increased, the oxygen purity is increased to 70 mg / L or higher at room temperature and atmospheric pressure, so that high concentration of oxygen can be consumed. Become. In addition, the existing oxygen water purifier is concerned about the propagation of bacteria as the water stays in the device, but the present invention can overcome this problem by giving a sterilization function.

<Examples 3 to 11> Treatment of Deep Sea Water

Example 3) A deep water was poured into a container with natural water pumped from the basement and the container was placed in a sterilization chamber to sterilize the filling. Here, the natural water and the deep sea water can be individually filled, but they can also be mixed in advance and filled. As the deep sea water, the range for the beverage is 10% or less and the preferred range is 5% or less and the optimum range is 3%.

Example 4) Containers were filled with natural water sprinkled underground and deep sea water, sweeteners and other additives. The vessel was then placed in a sterilization chamber and the filling was sterilized. Here, the natural water and the deep sea water may be individually filled, but may be pre-mixed and charged in a mixed state. Sweeteners and other additives are preferably mixed with natural water or deep sea water in advance. As the deep-sea deep-sea water, the range of employment is 10% or less, and the preferred range is 5% or less, and the optimal range is 3%.

Example 5) A container filled with natural water sprinkled underground and deep seawater concentrated in East Sea was placed in a sterilization chamber, and the filling was sterilized. Here, the deep sea water concentration is concentrated by reverse osmosis, electrodialysis or evaporation. As the concentrated deep sea water, the range of the beverage is 5% or less, and the preferred range is 3% or less.

Example 6 A container was charged with deep water from the basement and deep-salted East Sea deep water and placed in a sterilization chamber to sterilize the filling. Here, desalination is based on at least one treatment method from an electrodialysis method, a reverse osmosis membrane method, an ion exchange membrane method, and a distillation method. The beverage adoption range of the deep sea water is 1-99%.

Example 7) The deep sea water of the East Sea is evaporated by heating evaporation, vacuum evaporation, sun drying, ventilation drying, etc., and the evaporated dry matter obtained is mixed with natural water, natural water or distilled water pumped underground, and filled into the container, and the container is sterilized. And the filling was sterilized. Here, the range of employment for the beverage of evaporated dried water of deep sea water in East Sea is 2% or less, preferably 0.05-0.5%. If it is 2% or more, too much salt is contained and it is not suitable as a drink.

Example 8) Juices and concentrated East Sea deep water were charged to a vessel. The vessel was then placed in a sterilization chamber to sterilize the filling. When diluting the juice or the concentrated East Sea deep water with natural water, it is preferable that the salt concentration of the juice be within 5% depending on the amount of natural water used.

Example 9 A container was filled with natural water sprinkled underground, deep water desalted, deep sweetened water and other additives. Next, the container is placed in a sterilization chamber to sterilize the filling. The range where the desalination copper deep water can be employed is 1 to 99%, the preferred range is 5 to 50%, and the optimum range is 10 to 30%.

Example 10) The container was filled with concentrated deep seawater and desalted East Sea deep water, and sweeteners and other additives. The vessel was then placed in a sterilization chamber to sterilize the filling. The range where the desalination-concentrated East Sea deep water can be employed is 1 to 99%, the preferred range is 5 to 50%, and the optimum range is 10 to 30%.

Example 11 The container was filled with the natural water and the desalted deep-sea water, inorganic salts, and minerals which were raised underground. The vessel was placed in a sterile chamber and the filling was sterilized.

It is not limited to the said example using such deep-sea deep-sea water. For example, tap water, river water, or distilled water can be used as a substitute for natural water pumped up underground. In the case of desalting deep sea water, it is preferable that the deep sea water is desalted by at least one treatment method from an electrodialysis method, a reverse osmosis membrane method, an ion exchange membrane method, and a distillation method, leaving only components close to the body fluid. Concentration is dried by reverse osmosis, electrodialysis, evaporative concentration, etc. by heating evaporation, vacuum evaporation, sun drying, and ventilation drying. Other additives may be used to produce a soft drink using carbonic acid, or a beverage that increases dissolved oxygen by blowing oxygen into the desalted deep sea water. Demineralized water can also be used to make liquor such as beer, shochu, makgeolli, and fruit wine. As a means for filling the container of the beverage of the present invention, there are various means such as a method of filling each of the deep sea water and mineral water, etc., or mixing them in advance and filling them in a mixed state. Moreover, sterilization by hot steam, infrared rays, radiation, etc. are used as a filler sterilization means, for example.

<Example 12 to Example 14> Red ginseng beverage production 1

The present inventors prepared a red ginseng concentrate of 2 to 20 wt% red ginseng concentrate. At this time, the red ginseng concentrate is 3 ~ 10 wt% red ginseng concentrate is preferred. The deep water may be contained in an amount of 0.5 to 8 wt%, preferably 1 to 5 wt%. Oxygen water may be contained in the red ginseng beverages with an oxygen content of 12 ~ 150 mg / L, preferably contained in 25 ~ 100 mg / l.

Specifically, after mixing the red ginseng concentrate obtained by the extract method of Example 1 with water according to the composition of Examples 12 to 14 of Table 4 (using Mixer), the technical method of the patent PCT / KR2006 / 001774, that is, the membrane contactor Red ginseng beverages with oxygen were prepared by using (oxygen pressure 3bar, liquid pressure 2.5).

<Example 15 to Example 17> Red ginseng beverage production 2

The red ginseng concentrate obtained by the extraction method was mixed with water and deep water according to the composition of Examples 12 to 14 of Table 4 (using Mixer) to prepare a red ginseng beverage to which the deep water was added.

<Example 18 to Example 20> Red ginseng beverage production 3

The red ginseng concentrate obtained by the extract method was mixed with water and deep water according to the composition of Examples 12 to 14 of Table 2 (using Mixer), and then, using the technical method of Patent PCT / KR2006 / 001774, that is, a membrane contactor (oxygen pressure 3 bar, 2.5 g of liquid pressure to prepare a red ginseng beverage was added oxygen and deep water.

After preparing the red ginseng concentrate by the method described above, deep water, water and oxygen were prepared in the same composition as in Examples 12-20, and then tested by age group.

Example 12 Example 13 Example 14 Example 15 Example 16 Example 17 Example 18 Example 19 Example 20 Red ginseng concentrate (g) One 2 3 One 4 7 One 4 7 Water (g) 99 98 97 98 94 90 98 94 90 Deep water (g) One 2 3 One 2 3 Oxygen (mg / l) 30 45 60 30 45 60

The tasting test results for each age group for Examples 13, 16, and 19 of the Examples are shown in Tables 5 to 7.

Age group preferences (Example 13) Sensory test 20's 30 spaces 40 spaces 50 spaces 60 spaces incense ○ ● ☆ ☆ ☆ flavor ● ● ☆ ★ ★ color ★ ★ ● ● ★

Age group preferences (Example 16) Sensory test 20's 30 spaces 40 spaces 50 spaces 60 spaces incense ○ ● ☆ ★ ★ flavor ● ● ☆ ☆ ☆ color ● ● ● ☆ ★

Age group preferences (Example 19) Sensory test 20's 30 spaces 40 spaces 50 spaces 60 spaces incense ● ☆ ☆ ★ ★ flavor ● ★ ★ ★ ★ color ● ● ☆ ☆ ★

Satisfaction Survey on Red Ginseng Beverage

★ 90 ~ 100 Very satisfied

☆ 80 ~ 90 satisfaction

● 70-80 normal

○ 60 ~ 70 Poor

■ 50 ~ 60 complaints

□ 40 ~ 50 Very Complaint

Example 21 Individual Saponin Analysis of Red Ginseng Extract

We measured the saponin concentration using the individual saponin assay (SOP) in the red ginseng concentrate of the present invention.

The SOP was based on the test method for each ingredient in the draft notification (2007.10.1) of all the standards and standards of health functional foods, and this analysis manual was used in the red ginseng concentrate (liquid) as the SOP (Standard Operating Procedure) of the method. Detailed standard assay procedure for individual saponin assays.

Specifically, 1) acetonitrile (HPLC grade (Burdic & Jackson)), 2) distilled water (HPLC grade (Burdic & Jackson)), 3) standard ginsenosides Rb1 and Rg1 were purchased (KT & G CRM-002a, -001a). . 4) The standard solution was used by diluting the standard stock solution to an appropriate concentration using methanol. The standard stock solution and the standard solution were stored in a freezer (-20 ° C), and used at room temperature after shaking to reach room temperature.

The instrument is 1) 250 ml bottle, 2) Syringe filter (0.45 μm, PVDF), 3) HPLC solvent filtration filter (0.2 μm * Φ47 mm, nylon), 4) C18 column: Discovery C18 (4.6 mm id * 15 cm * 5 μm) was used. The instruments and devices used were 1) Balance (Mettler AE160) and 2) HPLC (Agilent 1200series).

In the test method, first, a test solution was prepared. About 2-4 g of the sample was precisely weighed and completely dissolved in 50 ml of water, and then filtered and used as a test solution. In the case of the nano sample was filtered and used directly as a test solution.

In the test operation, the measurement conditions of the high-performance liquid chromatograph are as follows.

1) Stationary phase: C18 column (Discovery C18, 4.6 ㎜ id * 15 ㎝ * 5 ㎛)

2) mobile phase: A, distilled water; B, acetonitrile (flow rate: 1.6 ml / min)

Minutes water(%) Acetonitrile (%) 0-10 80 20 10-40 80 → 68 20 → 32 40-48 68 → 58 32 → 42 48-50 58 → 0 42 → 100 50-60 0 100 60-62 0 → 80 100 → 20 62-70 80 20

NOTE The mobile phase ratio and flow rate can be changed to give optimum conditions such as peak resolution.

3) Injection amount: 20 μl

4) Detector: DAD (203 nm)

5) Column Temperature: 30 ℃

Quantitative tests are as follows. 20 μl of the standard solution and the test solution were injected, respectively, and tested under the above conditions. The concentration (mg / g) of ginsenosides Rb1 and Rg1 in the test solution was determined using the calibration curve determined by the area of the peak of the standard solution. At this time, the calculation is as follows.

Ginsenoside Rb1 content (mg / g) = Ginsenoside concentration in test solution = 4.286

Ginsenoside Rg1 content (mg / g) = Ginsenoside concentration in test solution = 0.506

<Example 22> Lymphocyte proliferation ability test of red ginseng concentrate

The present inventors prepared a red ginseng concentrate prepared by the method of Example 1.

Splenocytes from 6 to 8 weeks old BALB / c mice were harvested and dispensed with 1.5 X 10 ⁵ cells per 96 well flat-bottom microplate wells containing different doses for 72 hours at 37 ° C and 5% incubator. Incubated. After treatment with ³ H-Thymidine at 2 μCi / well, the cells were further incubated for 4 hours, the cells were harvested, scintillation cocktails were added, and cpm was measured by β-counter to obtain stimulation index (SI). Concanavalin A (Con A) was used as a standard. The stimulus index was calculated by the following equation.

Stimulation index of = Corporation (cpm) in the samples incubated spleen cells ³ H-Thymidine in Corporation (cpm) / ³ H-Thymidine in the spleen cells incubated with control culture medium of

The results are shown in Table 9 below.

Lymphocyte proliferation test results division Lymphocyte proliferation (SI) Control PBS: 1.0 Positive control group Con A: 40.9 (2.5 μg / ml) Example 1 22.4 (500 μg / ml)

As shown in Table 9, the lymphocyte proliferation capacity of the control group was 1.0, but Example 1 of the present invention was 22.4 (500 µg / ml). Therefore, the red ginseng concentrate of the present invention was found to be excellent lymphocyte proliferation ability.

Example 23 Lymphokine-Activated Killer Cell Production Performance of Red Ginseng Extract

Red ginseng concentrate prepared by the method of Example 1 of the present invention was prepared. LAK cell production capacity was used for 4 hours 51Cr release method.

Working cells were diluted in spleen cells with RPMI640-5% FBS medium to 3.0 X 10 cells / mL in a 24-well plate (Corning, New York, USA), followed by adding 30 U / mL of IL-2 or sample. 2 ml of each was incubated for 5 days at 37 ℃, 5% CO₂ incubator. After 5 days, 2 ml of the culture was transferred to a 15 ml tube, centrifuged to precipitate the cells, and washed twice with HBSS. The concentration of cells was fixed at 5 x 10 cells / ml. Adjust the ratio of the prepared and target cells to 100: 1, 30: 1 or 10: 1 in a 96-well U-bottomed microplate (Corning, New York, USA). Incubated for 4 hours at. After 4 hours, centrifugation was performed at 2,200 rpm for 5 minutes, and 0.1 ml of the supernatant was measured, and cytotoxicity was calculated by measuring the intensity of γ-rays by Cr released into the supernatant with γ-counter.

After calculating cytotoxicity by the following equation, LU was determined. Interleukin-2 (IL-2) was used as a standard.

Cytotoxicity (%) = ER-SR / MR-SR X 100

* ER: Experimental release count

SR: Spontaneous release count

MR: Maximum incorporation count

* LD10: means the number of effector cells capable of lysis of 10% target cells per million cells (higher value means more production of effector cells (LAK cell)).

The results are shown in Table 10 below.

LAK cell production test results division  LAK lytic units Control PBS: 1.0 Positive control group IL-2: 54.6 (30 units) Example 1 9.7 (100 μg / ml)

As shown in Table 10, the LAK cell production of the red ginseng concentrate of Example 1 of the present invention was confirmed to be 9.7 LU10 (100 ㎍ / ㎖), it was found that the effect is excellent.

1 is a process diagram schematically showing a method for preparing a red ginseng concentrate using red ginseng (6 years old) of the present invention,

2 is a spectrum showing the individual saponin (Ginsenoside) Rb1, Rg1 standard,

3 is a spectrum showing a Ginsenoside Rg3 standard,

4 is a spectrum showing the results of individual saponins of the red ginseng concentrate sample,

Figure 5 is a spectrum showing the fiber sample individual saponin test results of the red ginseng concentrate of the present invention,

6 shows a schematic system of an oxygen water production apparatus used in the present invention,

Figure 7 shows an example and the principle of implementation of the separator of the oxygen water production apparatus used in the present invention,

8 is a schematic view showing an embodiment of the adsorption method of the oxygen water production apparatus according to the present invention,

9 is a schematic view showing the principle of implementation of the contact membrane of the oxygen water production apparatus according to the present invention.

<Brief description of symbols used in the drawings>

1: contact membrane 2: oxygen generating means

3: two head compressor 4: oxygen tank

5: feed water bypass valve 6: water pressure measuring device

7: supply oxygen pressure gauge 8: compressed air pressure gauge

9: exhaust oxygen control valve 10: exhaust nitrogen control valve

11: oxygen water flow meter

12: dissolved oxygen measuring device

Claims (25)

delete Iii) screening and heat treating red ginseng as a raw material; Ii) mixing purified water with the selected red ginseng and extracting at high temperature; Iii) heating and sterilizing the extracted red ginseng solution; Iii) cooling the sterilized red ginseng extract and separating and removing impurities; Iii) concentrating and aging the red ginseng extract from which the impurities are removed; And Iii) the red ginseng extract prepared by the step of measuring the sugar and starch content of the red ginseng extract, Functional drink containing deep sea water and oxygen water. [3] The functional beverage of claim 2, wherein the sorting and heat treatment of step iii) are performed by three-sam / jeungsam / drying / shaping / screening process and a heat treatment process of 100 ~ 120 ℃. The functional beverage according to claim 2, wherein the purified water of step ii) is 4 to 8 times the mass of the raw red ginseng. The functional beverage of claim 2, wherein the high temperature of step ii) is 80 to 95 ° C by steam indirect heating. The functional beverage of claim 2, wherein the extraction of step ii) is performed 5-8 times in a continuous tank or 2-5 times in a batch tank. The functional beverage of claim 2, wherein the heating and sterilizing of step iii) is performed by heating and instant cooling / heating at 90 to 100 ° C. The functional beverage of claim 2, wherein the cooling of the step iii) is performed at 0 to 10 ° C. [Claim 3] The functional beverage of claim 2, wherein the removing of the impurities in the step iii) removes starch and foreign debris cooled by cooling at a flow rate of 10 to 30 l / min using a centrifuge at 5,000 to 8,000 rpm. . [Claim 3] The functional beverage according to claim 2, wherein the concentration and aging of the step iii) are carried out by concentrating by low temperature vacuum concentration method at 55-65 ° C. to remove impurities from the # 100-140 sieves, and then aging at least 10 hours. The method of claim 2, wherein the sugar and starch content of the step iii) is characterized in that the sugar content of 40 to 75 brix and 2 to 3% starch content. The method according to claim 2, wherein after the step iii), the chromaticity, water content and microbial analysis of the red ginseng concentrate are performed, wherein the chromaticity is 0.20 to 0.50, the water content is 30 to 45%, and the microorganism is 100 / g or less. A functional drink characterized by the above. The functional beverage according to claim 2, wherein the red ginseng serving as the raw material contains red ginseng root: red ginseng ratio of 1: 1 to 0.2. The functional beverage according to claim 2, wherein the functional beverage is prepared by adding deep sea water taken from 200 m or less at a depth of 0.1 to 5 times the mass of the red ginseng concentrate, and diluting it with 5 to 100 times purified water. . 15. The functional beverage of claim 14, wherein said deep sea water contains concentrated deep water. 15. The functional beverage according to claim 14, wherein the deep sea water of the East Sea contains the desalted deep water. The functional beverage according to claim 2, wherein the oxygen content of the oxygen water is 12 to 150 mg per liter of the functional beverage. The method of claim 2, wherein the oxygen water Oxygen generating means for separating oxygen from the supplied compressed air; A contact membrane supplied with oxygen generated from the oxygen generating means and dissolved in water; And And a second head receiving air and supplying the oxygen to the oxygen generating means and applying a vacuum to the oxygen generating means to increase the purity of the separated generated oxygen and simultaneously supplying the oxygen to the contact membrane under pressure. Functional drink characterized in that it is produced from the oxygen water production apparatus having a two-head compressor. 19. The functional beverage according to claim 18, wherein the oxygen generating means and the contact membrane of the oxygen water producing apparatus are porous membranes or nonporous membranes. 19. The functional beverage according to claim 18, wherein the oxygen generating means and the contact membrane of the oxygen water producing apparatus are hollow fiber type or flat membrane type. 19. The functional beverage according to claim 18, wherein the oxygen generating means of the oxygen water producing apparatus employs an oxygen cylinder, an adsorption method, or a separation membrane method. 19. The apparatus of claim 18, wherein the oxygen water production apparatus is supplied with air to a compressor for supplying oxygen to the oxygen generating means and a vacuum is applied to the oxygen generating means to increase the purity of the separated generated oxygen and at the same time to the contact membrane. A functional beverage further comprising a vacuum pump for supplying under pressure. 19. The functional beverage according to claim 18, wherein the oxygen water production device further comprises an oxygen bottle for storing oxygen water obtained through the contact membrane. 24. The functional beverage of claim 23, wherein the oxygen water producing device includes at least one of an oxygen water flow meter and a dissolved oxygen measuring device on one side of the oxygen water tank. The method of claim 2, wherein the oxygen water is compressed air and supplied to the oxygen generating means; Supplying oxygen separated from said oxygen generating means to a contact film; And dissolving oxygen by supplying water to the contact membrane.

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