CN111226910B - 卵子或卵裂期胚胎的玻璃化冷冻液及冷冻方法 - Google Patents
卵子或卵裂期胚胎的玻璃化冷冻液及冷冻方法 Download PDFInfo
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- CN111226910B CN111226910B CN202010157999.6A CN202010157999A CN111226910B CN 111226910 B CN111226910 B CN 111226910B CN 202010157999 A CN202010157999 A CN 202010157999A CN 111226910 B CN111226910 B CN 111226910B
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
Abstract
Description
卵子存活数(枚) | 存活卵子受精率 | |
实施例1 | 28 | 96.4% |
实施例2 | 23 | 91.3% |
实施例3 | 22 | 86.36% |
对比例1 | 25 | 88% |
对比例2 | 19 | 73.7% |
卵裂期胚胎存活数(枚) | 存活胚胎的分裂率 | |
实施例1 | 39 | 92.3% |
实施例2 | 34 | 88.2% |
实施例3 | 32 | 81.3% |
对比例1 | 34 | 85.3% |
对比例2 | 29 | 75.9% |
Claims (9)
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CN111602653B (zh) * | 2020-07-02 | 2021-03-02 | 深圳韦拓生物科技有限公司 | 一种玻璃化冷冻液套装及其制备方法 |
CN112772634B (zh) * | 2021-01-12 | 2022-07-29 | 台州灵江生物技术有限公司 | 植物蛋白多肽在玻璃化冷冻液中的应用、玻璃化冷冻液组合物及制备方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2003238620B2 (en) * | 2002-01-31 | 2008-11-06 | Asahi Techno Glass Corporation | Liquid for frozen storage of primate embryo stem cells and frozen storage method |
CN105052894A (zh) * | 2015-08-26 | 2015-11-18 | 中国农业科学院特产研究所 | 一种gv期卵母细胞冷冻保存液及冷冻保存方法 |
CN107232185A (zh) * | 2017-07-31 | 2017-10-10 | 青岛德瑞骏发生物科技股份有限公司 | 一种马属动物卵母细胞玻璃化冷冻保存液、冷冻保存方法和应用 |
CN110100813A (zh) * | 2019-06-06 | 2019-08-09 | 中国农业大学 | 一种羊胚胎玻璃化冷冻保存液配方及冷冻方法 |
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Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2003238620B2 (en) * | 2002-01-31 | 2008-11-06 | Asahi Techno Glass Corporation | Liquid for frozen storage of primate embryo stem cells and frozen storage method |
CN105052894A (zh) * | 2015-08-26 | 2015-11-18 | 中国农业科学院特产研究所 | 一种gv期卵母细胞冷冻保存液及冷冻保存方法 |
CN107232185A (zh) * | 2017-07-31 | 2017-10-10 | 青岛德瑞骏发生物科技股份有限公司 | 一种马属动物卵母细胞玻璃化冷冻保存液、冷冻保存方法和应用 |
CN110100813A (zh) * | 2019-06-06 | 2019-08-09 | 中国农业大学 | 一种羊胚胎玻璃化冷冻保存液配方及冷冻方法 |
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Effective date of registration: 20231127 Address after: Room 601, No. 6 Yonghe Yushan International Rongjing 1st Road, Xintang Town, Zengcheng District, Guangzhou City, Guangdong Province, 511300, self-made A1 Patentee after: GUANGZHOU FUKANG BIOTECHNOLOGY Co.,Ltd. Address before: 528300 room 413, 4th floor, block 22, Shunlian Machinery City, No.18, Xingye 4th Road, Guanglong Industrial Park, Chihua community, Chencun Town, Shunde District, Foshan City, Guangdong Province Patentee before: Foshan Fukang Biotechnology Co.,Ltd. |