CN104161037B - 一种山羊精液玻璃化冷冻和解冻配方与方法 - Google Patents
一种山羊精液玻璃化冷冻和解冻配方与方法 Download PDFInfo
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- CN104161037B CN104161037B CN201410399002.2A CN201410399002A CN104161037B CN 104161037 B CN104161037 B CN 104161037B CN 201410399002 A CN201410399002 A CN 201410399002A CN 104161037 B CN104161037 B CN 104161037B
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- 230000008014 freezing Effects 0.000 title claims abstract description 48
- 238000007710 freezing Methods 0.000 title claims abstract description 48
- 241000283707 Capra Species 0.000 title claims abstract description 23
- 239000011521 glass Substances 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 23
- 239000007788 liquid Substances 0.000 claims abstract description 50
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims abstract description 27
- 238000010257 thawing Methods 0.000 claims abstract description 22
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 9
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 9
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 9
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims abstract description 5
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims abstract description 5
- 239000001267 polyvinylpyrrolidone Substances 0.000 claims abstract description 5
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims abstract description 4
- 229940098773 bovine serum albumin Drugs 0.000 claims abstract description 4
- 210000000582 semen Anatomy 0.000 claims description 23
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 12
- 230000002792 vascular Effects 0.000 claims description 7
- 229910052757 nitrogen Inorganic materials 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 210000003462 vein Anatomy 0.000 claims description 3
- 238000007431 microscopic evaluation Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 5
- 230000035899 viability Effects 0.000 abstract description 2
- 241001494479 Pecora Species 0.000 description 13
- 239000000243 solution Substances 0.000 description 9
- 230000004899 motility Effects 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000019100 sperm motility Effects 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000004017 vitrification Methods 0.000 description 2
- 239000004606 Fillers/Extenders Substances 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 206010003883 azoospermia Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000036616 oligospermia Effects 0.000 description 1
- 208000008634 oligospermia Diseases 0.000 description 1
- 231100000528 oligospermia Toxicity 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000003879 sperm preservation Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 210000005239 tubule Anatomy 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Priority Applications (1)
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CN201410399002.2A CN104161037B (zh) | 2014-08-13 | 2014-08-13 | 一种山羊精液玻璃化冷冻和解冻配方与方法 |
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CN201410399002.2A CN104161037B (zh) | 2014-08-13 | 2014-08-13 | 一种山羊精液玻璃化冷冻和解冻配方与方法 |
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CN104161037A CN104161037A (zh) | 2014-11-26 |
CN104161037B true CN104161037B (zh) | 2016-01-13 |
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CN201410399002.2A Active CN104161037B (zh) | 2014-08-13 | 2014-08-13 | 一种山羊精液玻璃化冷冻和解冻配方与方法 |
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Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
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BR112017006533A2 (pt) * | 2014-09-29 | 2017-12-19 | Cook General Biotechnology Llc | usos de trealose em suspensões celulares |
CN111280166A (zh) * | 2020-04-08 | 2020-06-16 | 广州裕康生物科技有限公司 | 囊胚的玻璃化冷冻液及冷冻方法 |
CN111454883A (zh) * | 2020-04-08 | 2020-07-28 | 广州裕康生物科技有限公司 | 囊胚的玻璃化解冻液和解冻方法 |
CN114304138A (zh) * | 2022-01-17 | 2022-04-12 | 阿拉善盟畜牧兽医技术推广中心(阿拉善盟动物疫病预防控制中心) | 一种双峰驼的精液冷冻方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102657149A (zh) * | 2012-05-08 | 2012-09-12 | 山西农业大学 | 一种超高活力羊细管精液冷冻方法 |
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EP1675457B1 (en) * | 2003-09-16 | 2016-02-24 | 21st Century Medicine Inc. | Methods and compositions for the cryopreservation of organs |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN102657149A (zh) * | 2012-05-08 | 2012-09-12 | 山西农业大学 | 一种超高活力羊细管精液冷冻方法 |
Non-Patent Citations (1)
Title |
---|
"海藻糖应用于人类成熟卵母细胞玻璃化冷冻研究";邢琼等;《安徽医药》;20131231;第17卷(第12期);第2134-2137页 * |
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C14 | Grant of patent or utility model | ||
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TR01 | Transfer of patent right |
Effective date of registration: 20200109 Address after: 226100 group 17, Dahong village, Sanchang Town, Haimen City, Nantong City, Jiangsu Province Patentee after: Jiangsu Jinsheng goat breeding technology development Co.,Ltd. Address before: 212400 Zhenjiang City, Jiangsu Province Hangzhou Ning Jurong City No. 112 Patentee before: JIANGSU FOOTHILL ZHENJIANG AGRICULTURE Research Institute |
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TR01 | Transfer of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A formula and method for vitrification and thawing of goat semen Effective date of registration: 20210429 Granted publication date: 20160113 Pledgee: Jiangsu Haimen Rural Commercial Bank Co.,Ltd. Pledgor: Jiangsu Jinsheng goat breeding technology development Co.,Ltd. Registration number: Y2021320010157 |
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PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20220713 Granted publication date: 20160113 Pledgee: Jiangsu Haimen Rural Commercial Bank Co.,Ltd. Pledgor: Jiangsu Jinsheng goat breeding technology development Co.,Ltd. Registration number: Y2021320010157 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A kind of goat semen vitrification freezing and thawing formula and method Effective date of registration: 20220812 Granted publication date: 20160113 Pledgee: Jiangsu Haimen Rural Commercial Bank Co.,Ltd. Pledgor: Jiangsu Jinsheng goat breeding technology development Co.,Ltd. Registration number: Y2022320010463 |