CN111165494A - Seed treatment composition and use - Google Patents
Seed treatment composition and use Download PDFInfo
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- CN111165494A CN111165494A CN202010072130.1A CN202010072130A CN111165494A CN 111165494 A CN111165494 A CN 111165494A CN 202010072130 A CN202010072130 A CN 202010072130A CN 111165494 A CN111165494 A CN 111165494A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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Abstract
The invention discloses a seed treatment composition, which comprises active ingredients, wherein the active ingredients comprise organic macromolecular carbon sol, potassium permanganate and MS culture medium dry powder. The invention also provides the application of the seed treatment composition in low-temperature germination resistance of plants, promotion of root system development of the plants and late-stage senescence delay. The seed soaking, dressing or coating treatment is carried out by using the composition of the invention, thus not only improving the germination capacity of the seeds under various adversity stresses, but also improving the comprehensive resistance in the growth and development process, and also promoting the root growth of crops, regulating the crown-root ratio, delaying the later-stage aging and increasing the crop yield.
Description
Technical Field
The invention belongs to the technical field of crop production, particularly relates to a seed treatment composition and application, and particularly relates to a novel composition for improving germination of seeds under adversity stress, promoting development of lateral roots of corn roots, delaying later-stage senescence and improving corn yield.
Background
The external environmental conditions are important factors affecting seed germination. After the crops are sown, adverse conditions of soil such as low temperature, drought, salt, alkali stress and the like delay the germination process and reduce the germination rate; further leading to irregular emergence of seedlings, even seedling shortage and ridge breaking, seriously influencing the growth and development of crops and the formation of yield and leading to the reduction of the crop yield.
Seed treatment techniques prior to sowing include physical and chemical methods. Physical methods such as patent ZL201720656220.9 describe methods and devices for improving the germination capacity of seeds by means of ultrasonic treatment. Also as described in patent 200880009934.4, a method for improving germination capacity of seeds by high frequency pulsed electric field treatment is disclosed. The chemical method mainly utilizes chemical agents to improve the capability of resisting diseases and insect pests, improve the seed activity and promote the seed germination through seed soaking, seed dressing and coating. The main components of the chemical agents are one or more of bactericides, insecticides, plant nutrients and plant hormones. For example, patent 201510937960.5 provides a reagent composition for seed treatment comprising proline, gibberellin and cytokinin; the seed treatment agent provided by patent 201280052973.9 is a lipochitooligosaccharide; the seed treatment agent provided by patent 201280064006.4 is natamycin; the main components and other auxiliary agents including film forming agent, filling agent, etc. are used to coat the seed. However, there are few seed treatment techniques that improve seed germination resistance to various adversity stresses such as temperature, moisture, salinity and alkalinity. Therefore, there is a great need for new compositions that improve the overall resistance of seed germination.
Disclosure of Invention
An object of the present invention is to solve at least the above problems and/or disadvantages and to provide at least the advantages described hereinafter.
It is also an object of the present invention to provide a seed treatment composition.
It is a further object of the present invention to provide the use of a seed treatment composition.
Therefore, the technical scheme provided by the invention is as follows:
a seed treatment composition comprising an active ingredient comprising the following components in the following concentrations: 0.3-3.5% (V/V) of organic macromolecular carbon sol, 6-60mg/L of potassium permanganate and 2-10g/L of MS culture medium dry powder.
Preferably, in the seed treatment composition, the concentration of the organic macromolecular carbon sol is 0.5-3% (V/V), the concentration of potassium permanganate is 10-50mg/L, and the concentration of MS culture medium dry powder is 4-8 g/L. More preferably, in the seed treatment composition, the concentration of the organic macromolecular carbon sol is 2.5-3.5% (V/V), the concentration of potassium permanganate is 40-60mg/L, and the concentration of MS culture medium dry powder is 6-10 g/L. Most preferably, in the seed treatment composition, the concentration of the organic macromolecular carbon sol is 3% (V/V), the concentration of potassium permanganate is 50mg/L, and the concentration of MS culture medium dry powder is 8 g/L. The composition has the functions of promoting the development of corn root systems and delaying the late senescence.
Preferably, in the seed treatment composition, the active ingredients further comprise: one or more of salicylic acid, methyl jasmonate and abscisic acid. The three hormones can further enhance the effect of the plant root system on the regulation and control of the organic macromolecular carbon sol.
Preferably, the seed treatment composition further comprises a binder, wherein the binder is any one or more of gelatin, polyvinylpyrrolidone K40, polyvinylpyrrolidone K60, hydroxyethyl cellulose and sodium carboxymethyl cellulose.
Preferably, in the seed treatment composition, the binder comprises polyvinylpyrrolidone K60 at a concentration of 12-15g/L and a gelatin mixture at a concentration of 1.2-1.8 g/L. The composition can be used as seed dressing agent with optimal effect.
Preferably, in the seed treatment composition, the binder comprises polyvinylpyrrolidone K40 at a concentration of 2-7g/L, hydroxyethyl cellulose at a concentration of 10-20g/L, and sodium carboxymethyl cellulose at a concentration of 10-20 g/L. The composition can be used as seed coating composition with optimal effect.
Preferably, the seed treatment composition further comprises a dispersing agent and a coloring agent, wherein the dispersing agent comprises diatomite with the concentration of 30-60g/L, and the diatomite has strong moisture absorption capacity and can absorb water in soil to facilitate the germination of seeds. The colorant comprises acid bright red with a concentration of 40-60 g/L.
Preferably, in the seed treatment composition, the concentration of the salicylic acid is 30-160mg/L, the concentration of the methyl jasmonate is 0.2-1mM, and the concentration of the abscisic acid is 20-100 mg/L.
Preferably, in the seed treatment composition, the composition is used in a manner of soaking, dressing or coating the seed.
The seed treatment composition is used for resisting low-temperature germination of plants, promoting root system development of the plants and delaying later-stage senescence.
The invention at least comprises the following beneficial effects:
in the invention, the organic macromolecular carbon sol is sol-state macromolecular organic carbon which is artificially synthesized by an electrochemical method and is rich in hydroxyl and carboxyl functional groups; the organic macromolecular carbon sol has weak oxidation activity and weak reduction activity simultaneously; the invention is used for activating the gene regulation network of the environmental adaptive response of the plant and inducing the cold resistance of the plant to increase. The invention selects potassium permanganate as a bactericide, and can effectively kill bacteria and fungi on the surfaces of various seeds by oxidizing active groups of thalli. The MS culture medium dry powder provides sufficient nutrient supply for seed germination. The three components are used cooperatively, so that the germination capacity of the seeds at low temperature is obviously improved, the seedling emergence time under the field condition is also advanced, the germination rate is also obviously improved, and the early seedling emergence of the crop seeds under the low-temperature condition after sowing is facilitated. Meanwhile, the composition can improve the ear number of a single plant and remarkably improve the yield. In addition, after the corn seeds are treated by the composition, the organic macromolecular carbon sol is contacted with the corn root system and then is combined with a specific receptor on the surface of a root system cell to activate a stress response gene regulation network of the corn root system, so that the development of lateral roots is promoted, the surface area and the dry weight of the root system are increased, the activity of the root system is increased, the growth and the development of the overground part of the corn are promoted, the senescence of the photosynthetic function of leaves is delayed, and the yield of the corn is increased.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Drawings
FIG. 1 is one of photographs showing the effect on corn seedling growth after seed coating treatment with the composition according to one embodiment of the present invention;
FIG. 2 is a second photograph showing the effect on corn seedling growth after seed coating treatment with the composition according to one embodiment of the present invention.
Detailed Description
The present invention is further described in detail below with reference to the attached drawings so that those skilled in the art can implement the invention by referring to the description text.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
The present invention provides a seed treatment composition having a low temperature germination resistance, comprising active ingredients comprising the following components in the following concentrations: 0.3-3.5% (V/V) of organic macromolecular carbon sol, 6-60mg/L of potassium permanganate and 2-10g/L of MS culture medium dry powder. Organic macromolecular carbon solIs a sol-state macromolecular organic carbon which is artificially synthesized by an electrochemical method and is rich in hydroxyl and carboxyl functional groups; the organic macromolecular carbon sol has weak oxidation activity and weak reduction activity simultaneously; the invention is used for activating the gene regulation network of the environmental adaptive response of the plant and inducing the cold resistance of the plant to increase. The molecular formula of the potassium permanganate is KMnO4And the molecular weight is 158.03. The melting point is 240 ℃, black purple, thin prismatic crystals or particles, bluish metallic luster, easy water solubility and stability. The invention selects the bacillus subtilis as a bactericide, and can effectively kill bacteria and fungi on the surfaces of various seeds by oxidizing active groups of thalli. The MS culture medium dry powder contains various large-scale and trace element components of the MS culture medium, and can provide sufficient nutrient supply for seed germination. The MS culture medium dry powder in the invention is a product of Duchefa Biochemie company. The three components are used cooperatively, so that the germination capacity of the seeds at low temperature is obviously improved, the seedling emergence time under the field condition is also advanced, the germination rate is also obviously improved, and the early seedling emergence of the crop seeds under the low-temperature condition after sowing is facilitated. Meanwhile, the composition can improve the ear number of a single plant and remarkably improve the yield.
In one embodiment of the invention, preferably, the concentration of the organic macromolecular carbon sol is 0.5-3% (V/V), the concentration of potassium permanganate is 10-50mg/L, and the concentration of MS culture medium dry powder is 4-8 g/L.
In the scheme, preferably, the concentration of the organic macromolecular carbon sol is 2.5-3.5% (V/V), the concentration of potassium permanganate is 40-60mg/L, and the concentration of MS culture medium dry powder is 6-10 g/L. Most preferably, in the seed treatment composition, the concentration of the organic macromolecular carbon sol is 3% (V/V), the concentration of potassium permanganate is 50mg/L, and the concentration of MS culture medium dry powder is 8 g/L. The composition has the functions of promoting the development of corn root systems and delaying the late senescence. After the corn seeds germinate, the organic macromolecular carbon sol contacts the corn root system and is combined with a specific receptor on the cell surface of the root system to activate a stress response gene regulation and control network of the corn root system, so that the development of lateral roots is promoted, the surface area and the dry weight of the root system are increased, the activity of the root system is increased, the growth and development of the overground part of the corn are promoted, the senescence of the photosynthetic function of the leaves is delayed, and the yield of the corn is improved.
In one embodiment of the present invention, preferably, the active ingredient further comprises: one or more of salicylic acid, methyl jasmonate and abscisic acid. The three hormones can further enhance the effect of the plant root system on the regulation and control of the nanocarbon sol.
In one embodiment of the present invention, the composition further comprises a binder, wherein the binder is any one or more of gelatin, polyvinylpyrrolidone K40, polyvinylpyrrolidone K60, hydroxyethyl cellulose and sodium carboxymethyl cellulose.
In one embodiment of the invention, the binder preferably comprises polyvinylpyrrolidone K60 in a concentration of 12-15g/L and a gelatin mixture in a concentration of 1.2-1.8 g/L. The composition can be used as seed dressing agent with optimal effect.
In one embodiment of the invention, the binder preferably comprises polyvinylpyrrolidone K40 at a concentration of 2-7g/L, hydroxyethyl cellulose at a concentration of 10-20g/L, and sodium carboxymethyl cellulose at a concentration of 10-20 g/L. The composition can be used as seed coating composition with optimal effect.
In one embodiment of the present invention, the composition further comprises a dispersant and a colorant, wherein the dispersant comprises diatomite with a concentration of 30-60g/L, and the diatomite has strong moisture absorption capacity and can absorb moisture in soil to facilitate germination of seeds. The colorant comprises acid bright red with a concentration of 40-60 g/L.
In one embodiment of the invention, preferably, the concentration of the salicylic acid is 30-160mg/L, the concentration of the methyl jasmonate is 0.2-1mM, and the concentration of the abscisic acid is 20-100 mg/L.
In one embodiment of the present invention, the composition is preferably used in the form of seed soaking, dressing or coating of seeds. Preferably, the seed soaking treatment uses active ingredients comprising: 0.3-3.5% (V/V) of organic macromolecular carbon sol, 6-20mg/L of potassium permanganate and 2-6g/L of MS culture medium dry powder. In seed dressing treatment, active ingredients used comprise: 0.3-3.5% (V/V) of organic macromolecular carbon sol, 10-30mg/L of potassium permanganate and 6-10g/L of MS culture medium dry powder. In the coating treatment, active ingredients comprising: 0.3-3.5% (V/V) of organic macromolecular carbon sol, 40-60mg/L of potassium permanganate and 6-10g/L of MS culture medium dry powder.
In one embodiment of the present invention, the seed soaking treatment method using the composition preferably comprises: preparing an aqueous solution containing the active ingredients of the composition, putting the solution into a container, preserving heat in a water bath at 35-45 ℃, adding a proper amount of seeds into the solution of the composition, preserving heat, soaking the seeds for 1-3 hours, taking out and airing for later use.
In one embodiment of the present invention, the seed dressing treatment method using the composition preferably comprises: preparing an aqueous solution containing the active ingredients of the composition and a binding agent, carrying out seed dressing treatment on seeds according to the proportion of adding 2-10mL of seed dressing agent into 1000g of seeds, and airing for later use after seed dressing.
In one embodiment of the present invention, the method for seed coating treatment using the composition preferably comprises: preparing an aqueous solution containing the active ingredients of the composition, a binder, a dispersant and a colorant, coating the seeds according to the proportion of adding 1-3mL of the seed coating composition into 1000g of the seeds, and airing for later use after coating.
The invention also provides application of the seed treatment composition in low-temperature germination resistance of plants, promotion of root system development of the plants and late senescence delay.
In order to make the technical solution of the present invention better understood by those skilled in the art, the following examples are now provided for illustration:
example 1
The formulation of this example was used to seed-soak seeds and included: 0.5% of organic macromolecular carbon sol, 4g/L of MS culture medium dry powder and 10mg/L of potassium permanganate. 5mL of organic macromolecular carbon sol, 1mL of 10g/L potassium permanganate mother liquor and 4g of MS culture medium dry powder are added into 1L of water and fully dissolved to obtain the composition in the embodiment.
According to the formula, rice (Jingen 45), corn (Zhongmai 909) and wheat (Zhongmai 895) seeds are respectively soaked, and clear water is used as a control. The treated seeds were subjected to germination tests in petri dishes. The test set two temperature treatments, 15 ℃ and 26 ℃ for rice and corn and 4 ℃ and 20 ℃ for wheat.
TABLE 1 Effect of seed soaking treatment on germination percentage of corn, rice and wheat at Low temperature
As can be seen from Table 1, the germination vigor under low-temperature growth conditions is significantly improved by applying the composition to seed soaking treatment, the germination rates of seeds which are not tolerant to low temperature such as rice and corn are also significantly improved, and the effect of the composition treatment under normal temperature conditions is small. Tests show that the seed soaking treatment by using the composition obviously improves the germination capacity of seeds at low temperature, and is beneficial to early seedling emergence of crop seeds at low temperature after sowing.
Example 2
The formulation of this example was used to seed dressing treatment of seeds comprising: 1% of organic macromolecular carbon sol, 8g/L of MS culture medium dry powder, 20mg/L of potassium permanganate, 10g/L of polyvinylpyrrolidone K60 and 1.5g/L of gelatin.
Accurately weighing 10g of polyvinylpyrrolidone K60, adding the polyvinylpyrrolidone K60 into 800mL of water, stirring to fully dissolve, then weighing 1.5g of gelatin, adding the gelatin into the solution, stirring to dissolve, adding water to a constant volume of 1000mL, and preparing into a binder for later use; and adding 10mL of organic macromolecular carbon sol, 5mL of 10g/L potassium permanganate mother solution and 8g of MS culture medium dry powder into the adhesive, and fully dissolving.
According to the formula, seeds of rice (Jingen 45), corn (Zhongmai 909) and wheat (Zhongmai 895) are subjected to seed dressing treatment. And (3) carrying out seed dressing treatment on the seeds according to the proportion of adding 5mL of seed dressing agent into 1000g of seeds, and airing for later use after seed dressing. The treated seeds are sown and the seedling emergence condition is tested in a shelter test base of the Chinese academy of agricultural sciences crop science research institute. The corn is sowed in the field in 10 days after 4 months, and the corn is sowed with single seed, the plant spacing is 27cm, and the row spacing is 60 cm. The rice is subjected to a germination test by using a seedling raising tray, the seeding period is 4 months and 10 days, and the seeding amount is 100 grains/square decimeter; sowing wheat in field in 5 days of 11 months, and sowing in holes with 3 grains per hole and 5cm hole distance. The sowing date of the rice and the corn is 15-20 days earlier than the normal sowing date, and the sowing date of the wheat is 30-40 days later. The results are shown in Table 2.
TABLE 2 Effect of seed dressing treatment on emergence of seedlings at Low temperatures for corn, Rice and wheat
Example 3
The formulation of this example was used to coat seeds and comprised: 3% of organic macromolecular carbon sol, 8g/L of MS culture medium dry powder, 50mg/L of potassium permanganate, 5g/L of polyvinylpyrrolidone K40, 15g/L of hydroxyethyl cellulose (5000-6400 MPa.S), 15g/L of sodium carboxymethylcellulose (800-1200 MPa.S), 60g/L of diatomite and 50g/L of acid scarlet;
accurately weighing 5g of polyvinylpyrrolidone K40, adding the polyvinylpyrrolidone K40 into 950mL of water, heating and stirring the mixture to be fully dissolved, then weighing 15g of hydroxyethyl cellulose (5000-6400 MPa.S) and 15g of sodium carboxymethyl cellulose (800-1200 MPa.S) and adding the mixture into the solution while stirring to prepare a binder for later use; then 60g of diatomite and 50g of acid scarlet are added into the adhesive, after the components are uniformly stirred, 30mL of organic macromolecular carbon sol, 5mL of 10g/L potassium permanganate mother liquor and 8g of MS culture medium dry powder are added into the semi-flowable mixture, and the mixture is stirred to be sufficiently and uniformly.
According to the formula, seeds of rice (Jingen 45), corn (Zhongmai 909) and wheat (Zhongmai 895) are subjected to seed coating treatment. And (3) coating the seeds according to the proportion that 1000g of seeds are added with 2mL of seed coating composition, and airing for later use after coating. The test of the sowing and seedling emergence conditions of the coated seeds is carried out in a shelter test base of the crop science research institute of the Chinese academy of agricultural sciences. The corn is sowed in the field in 10 days after 4 months, and the corn is sowed with single seed, the plant spacing is 27cm, and the row spacing is 60 cm. The rice is subjected to a germination test by using a seedling raising tray, the seeding period is 4 months and 10 days, and the seeding amount is 100 grains/square decimeter; sowing wheat in field in 5 days of 11 months, and sowing in holes with 3 grains per hole and 5cm hole distance. The sowing date of the rice and the corn is 15-20 days earlier than the normal sowing date, and the sowing date of the wheat is 30-40 days later. The results are shown in Table 3.
TABLE 3 Effect of seed coating treatment composition on emergence of seedlings at Low temperatures in corn, Rice and wheat
| Sowing time | Stage of emergence | The rate of emergence% | ||
| Corn (corn) | Control | 04/10 | 04/25 | 81 |
| Treatment of | 04/10 | 04/20 | 93 | |
| Rice (Oryza sativa L.) with improved resistance to stress | Control | 04/10 | 04/27 | 66 |
| Treatment of | 04/10 | 04/22 | 87 | |
| Wheat (Triticum aestivum L.) | Control | 11/05 | 11/18 | 91 |
| Treatment of | 11/05 | 11/16 | 100 |
As can be seen from Table 3, the seed coating treatment of the seed coating treatment composition using the composition significantly improves the germination capacity of corn, rice and wheat seeds at low temperature, advances the emergence of seedlings under field conditions, and significantly improves the germination rate.
Example 4
The seed coating treatment composition prepared according to the specific example 3 was applied to the rice seeds of 3 lines, and the field dry direct seeding experiment was performed in the city of public main mountains of Jilin province. Table 4 shows the number of basic seedlings after the seed coating treatment composition treatment, and it can be seen that the seed coating treatment of the composition provided by the invention can improve the field germination rate and promote tillering to occur, and the number of basic seedlings is significantly higher than that of the control, see table 4. Yield composition analysis (table 5) shows that the seed coating treatment of the composition provided by the invention mainly improves the number of ears per plant, and the actual yield measured in a cell is also obviously improved.
TABLE 4 Effect of seed coating treatment composition treatment on the number of basal seedlings in different varieties of the field
TABLE 5 Effect of seed coating treatment composition treatment on direct seeded rice yield and yield constitution
Example 5
The formulation of this example was used to seed-soak seeds and included: 0.3 percent of organic macromolecular carbon sol, 2g/L of MS culture medium dry powder and 6mg/L of potassium permanganate. The formulation was prepared according to the method of example 1.
According to the formula, rice (Jingen 45), corn (Zhongmai 909) and wheat (Zhongmai 895) seeds are respectively soaked, and clear water is used as a control. The seed is soaked in the liquid surface of the seed. The treated seeds were subjected to germination tests in petri dishes. The test set two temperature treatments, 15 ℃ and 26 ℃ for rice and corn and 4 ℃ and 20 ℃ for wheat. The results are shown in Table 6.
TABLE 6 influence of seed soaking treatment on germination rates of corn, rice and wheat at Low temperatures
Example 6
The formulation of this example was used to seed-soak seeds and included: 3.5 percent of organic macromolecular carbon sol, 6g/L of MS culture medium dry powder and 20mg/L of potassium permanganate. The formulation was prepared according to the method of example 1.
According to the formula, rice (Jingen 45), corn (Zhongmai 909) and wheat (Zhongmai 895) seeds are respectively soaked, and clear water is used as a control. The seed is soaked in the liquid surface of the seed. The treated seeds were subjected to germination tests in petri dishes. The test set two temperature treatments, 15 ℃ and 26 ℃ for rice and corn and 4 ℃ and 20 ℃ for wheat. The results are shown in Table 7.
TABLE 7 Effect of seed soaking treatment on germination percentage of corn, rice and wheat at Low temperature
Example 7
The formulation of this example was used to seed dressing treatment of seeds comprising: 1.5% of organic macromolecular carbon sol, 6g/L of MS culture medium dry powder, 30mg/L of potassium permanganate, 12g/L of polyvinylpyrrolidone K60 and 1.2g/L of gelatin. The formulation was prepared according to the method of example 2.
According to the formula, seeds of rice (Jingen 45), corn (Zhongmai 909) and wheat (Zhongmai 895) are subjected to seed dressing treatment. And (3) carrying out seed dressing treatment on the seeds according to the proportion of adding 10mL of seed dressing agent into 1000g of seeds, and airing for later use after seed dressing. The treated seeds were sown in the veranda test base of the institute of crop science of the academy of agricultural sciences of china, and the sowing method and field management were the same as those in example 2. The results are shown in Table 8.
TABLE 8 Effect of seed dressing treatment on emergence of seedlings at Low temperatures for corn, Rice and wheat
| Sowing time | Stage of emergence | The rate of emergence% | ||
| Corn (corn) | Control | 04/10 | 04/25 | 74 |
| Treatment of | 04/10 | 04/22 | 86 | |
| Rice (Oryza sativa L.) with improved resistance to stress | Control | 04/10 | 04/27 | 61 |
| Treatment of | 04/10 | 04/24 | 79 | |
| Wheat (Triticum aestivum L.) | Control | 11/05 | 11/18 | 82 |
| Treatment of | 11/05 | 11/17 | 96 |
Example 8
The formulation of this example was used to seed dressing treatment of seeds comprising: 2% of organic macromolecular carbon sol, 10g/L of MS culture medium dry powder, 10mg/L of potassium permanganate, 15g/L of polyvinylpyrrolidone K60 and 1.8g/L of gelatin. The formulation was prepared according to the method of example 2.
According to the formula, seeds of rice (Jingen 45), corn (Zhongmai 909) and wheat (Zhongmai 895) are subjected to seed dressing treatment. And (3) carrying out seed dressing treatment on the seeds according to the proportion of adding 2mL of seed dressing agent into 1000g of seeds, and airing for later use after seed dressing. The treated seeds were tested for emergence at the shelter test base of the institute of crop science of the academy of agricultural sciences, and the sowing method and field management were the same as those in example 2. The results are shown in Table 9.
TABLE 9 Effect of seed dressing treatment on emergence of seedlings at Low temperatures for corn, Rice and wheat
| Sowing time | Stage of emergence | The rate of emergence% | ||
| Corn (corn) | Control | 04/10 | 04/25 | 74 |
| Treatment of | 04/10 | 04/22 | 88 | |
| Rice (Oryza sativa L.) with improved resistance to stress | Control | 04/10 | 04/27 | 62 |
| Treatment of | 04/10 | 04/24 | 80 | |
| Wheat (Triticum aestivum L.) | Control | 11/05 | 11/18 | 83 |
| Treatment of | 11/05 | 11/17 | 97 |
Example 9
The formulation of this example was used to coat seeds and comprised: 3.5% of organic macromolecular carbon sol, 10g/L of MS culture medium dry powder, 60mg/L of potassium permanganate, 2g/L of polyvinylpyrrolidone K40, 10g/L of hydroxyethyl cellulose (5000-6400 MPa.S), 10g/L of sodium carboxymethyl cellulose (800-1200 MPa.S), 30g/L of diatomite and 40g/L of acid scarlet. The formulation was prepared according to the method of example 3.
According to the formula, seeds of rice (Jingen 45), corn (Zhongmai 909) and wheat (Zhongmai 895) are subjected to seed coating treatment. Coating the seeds according to the proportion that 1000g of seeds are added with 1mL of seed coating composition, and drying for later use after coating. The seeds after coating were sown in the veranda test base of the institute of crop science of academy of agricultural sciences of china in the same manner as in example 3. The results are shown in Table 10.
TABLE 10 Effect of seed coating treatment composition coating treatment on emergence of seedlings at Low temperatures in corn, Rice and wheat
| Sowing time | Stage of emergence | The rate of emergence% | ||
| Corn (corn) | Control | 04/10 | 04/25 | 79 |
| Treatment of | 04/10 | 04/20 | 91 | |
| Rice (Oryza sativa L.) with improved resistance to stress | Control | 04/10 | 04/27 | 63 |
| Treatment of | 04/10 | 04/22 | 85 | |
| Wheat (Triticum aestivum L.) | Control | 11/05 | 11/18 | 90 |
| Treatment of | 11/05 | 11/16 | 100 |
Example 10
The formulation of this example was used to coat seeds and comprised: 2.5 percent of organic macromolecular carbon sol, 6g/L of MS culture medium dry powder, 40mg/L of potassium permanganate, 7g/L of polyvinylpyrrolidone K40, 20g/L of hydroxyethyl cellulose (5000-6400 MPa.S), 20g/L of sodium carboxymethyl cellulose (800-1200 MPa.S), 70g/L of diatomite and 60g/L of acid scarlet. The formulation was prepared according to the method of example 3.
According to the formula, seeds of rice (Jingen 45), corn (Zhongmai 909) and wheat (Zhongmai 895) are subjected to seed coating treatment. And (3) coating the seeds according to the proportion that 1000g of seeds are added into 3mL of the seed coating composition, and airing for later use after coating. The seeds after coating were sown in the veranda test base of the institute of crop science of academy of agricultural sciences of china in the same manner as in example 3. The results are shown in Table 11.
TABLE 11 Effect of seed coating treatment composition coating treatment on Low temperature emergence of corn, Rice and wheat
| Sowing time | Stage of emergence | The rate of emergence% | ||
| Corn (corn) | Control | 04/10 | 04/25 | 77 |
| Treatment of | 04/10 | 04/20 | 91 | |
| Rice (Oryza sativa L.) with improved resistance to stress | Control | 04/10 | 04/27 | 62 |
| Treatment of | 04/10 | 04/22 | 85 | |
| Wheat (Triticum aestivum L.) | Control | 11/05 | 11/18 | 90 |
| Treatment of | 11/05 | 11/16 | 100 |
Surprisingly, in subsequent studies, the applicant found that the composition of the present invention also has the effects of promoting the development of the corn root system and delaying the late-stage aging, which is illustrated by the formula of example 3 and 11-24:
example 11
This example was used for coating seeds with the following formulation: 3% of organic macromolecular carbon sol, 8g/L of MS culture medium dry powder, 50mg/L of potassium permanganate, 32mg/L of salicylic acid, 5g/L of polyvinylpyrrolidone K40, 15g/L of hydroxyethyl cellulose (5000-6400 MPa.S), 15g/L of sodium carboxymethyl cellulose (800-1200 MPa.S), 60g/L of diatomite and 50g/L of acid scarlet.
100mL of the solution prepared in example 3 was added to 0.32mL of a salicylic acid mother solution (10 mg/mL), and the mixture was stirred sufficiently and homogeneously.
Example 12
This example was used for coating seeds with the following formulation: 3% of organic macromolecular carbon sol, 8g/L of MS culture medium dry powder, 50mg/L of potassium permanganate, 80mg/L of salicylic acid, 5g/L of polyvinylpyrrolidone K40, 15g/L of hydroxyethyl cellulose (5000-6400 MPa.S), 15g/L of sodium carboxymethyl cellulose (800-1200 MPa.S), 60g/L of diatomite and 50g/L of acid scarlet.
100mL of the solution prepared in example 3 was added to 0.8mL of a salicylic acid mother solution (10 mg/mL), and the mixture was stirred sufficiently and homogeneously.
Example 13
This example was used for coating seeds with the following formulation: 3% of organic macromolecular carbon sol, 8g/L of MS culture medium dry powder, 50mg/L of potassium permanganate, 160mg/L of salicylic acid, 5g/L of polyvinylpyrrolidone K40, 15g/L of hydroxyethyl cellulose (5000-6400 MPa.S), 15g/L of sodium carboxymethyl cellulose (800-1200 MPa.S), 60g/L of diatomite and 50g/L of acid scarlet.
100mL of the solution prepared in example 3 was added to 1.6mL of a salicylic acid mother solution (10 mg/mL), and the mixture was stirred sufficiently and homogeneously.
Example 14
This example was used for coating seeds with the following formulation: 3% of organic macromolecular carbon sol, 8g/L of MS culture medium dry powder, 50mg/L of potassium permanganate, 0.2mM of methyl jasmonate, 5g/L of polyvinylpyrrolidone K40, 15g/L of hydroxyethyl cellulose (5000-6400 MPa.S), 15g/L of sodium carboxymethyl cellulose (800-1200 MPa.S), 60g/L of diatomite and 50g/L of acid scarlet.
100mL of the solution prepared in example 3 was added to 0.2mL of 100mM methyl jasmonate mother liquor, and the mixture was stirred sufficiently uniformly.
Example 15
The coating treatment recipe for the seeds of this example was: 3% of organic macromolecular carbon sol, 8g/L of MS culture medium dry powder, 50mg/L of potassium permanganate, 0.6mM of methyl jasmonate, 5g/L of polyvinylpyrrolidone K40, 15g/L of hydroxyethyl cellulose (5000-6400 MPa.S), 15g/L of sodium carboxymethyl cellulose (800-1200 MPa.S), 60g/L of diatomite and 50g/L of acid scarlet.
100mL of the solution prepared in example 3 was added to 0.6mL of 100mM methyl jasmonate mother liquor, and the mixture was stirred sufficiently uniformly.
Example 16
The coating treatment recipe for the seeds of this example was: 3% of organic macromolecular carbon sol, 8g/L of MS culture medium dry powder, 50mg/L of potassium permanganate, 1mM of methyl jasmonate, 5g/L of polyvinylpyrrolidone K40, 15g/L of hydroxyethyl cellulose (5000-6400 MPa.S), 15g/L of sodium carboxymethyl cellulose (800-1200 MPa.S), 60g/L of diatomite and 50g/L of acid scarlet.
100mL of the solution prepared in example 3 was added to 1mL of 100mM methyl jasmonate mother liquor, and the mixture was stirred sufficiently uniformly.
Example 17
This example was used for coating seeds with the following formulation: 3% of organic macromolecular carbon sol, 8g/L of MS culture medium dry powder, 50mg/L of potassium permanganate, 20mg/L of abscisic acid, 5g/L of polyvinylpyrrolidone K40, 15g/L of hydroxyethyl cellulose (5000-6400 MPa.S), 15g/L of sodium carboxymethyl cellulose (800-1200 MPa.S), 60g/L of diatomite and 50g/L of acid scarlet;
100mL of the solution prepared in example 3 was added to 0.2mL of an abscisic acid mother liquor (10 mg/mL), and the mixture was stirred sufficiently and homogeneously.
Example 18
This example was used for coating seeds with the following formulation: 3% of organic macromolecular carbon sol, 8g/L of MS culture medium dry powder, 50mg/L of potassium permanganate, 60mg/L of abscisic acid, 5g/L of polyvinylpyrrolidone K40, 15g/L of hydroxyethyl cellulose (5000-6400 MPa.S), 15g/L of sodium carboxymethyl cellulose (800-1200 MPa.S), 60g/L of diatomite and 50g/L of acid scarlet;
100mL of the solution prepared in example 3 was added to 0.6mL of an abscisic acid mother liquor (10 mg/mL), and the mixture was stirred sufficiently and homogeneously.
Example 19
This example was used for coating seeds with the following formulation: 3% of organic macromolecular carbon sol, 8g/L of MS culture medium dry powder, 50mg/L of potassium permanganate, 100mg/L of abscisic acid, 5g/L of polyvinylpyrrolidone K40, 15g/L of hydroxyethyl cellulose (5000-6400 MPa.S), 15g/L of sodium carboxymethyl cellulose (800-1200 MPa.S), 60g/L of diatomite and 50g/L of acid scarlet;
100mL of the solution prepared in example 3 was taken, and 1mL of an abscisic acid mother liquor (10 mg/mL) was added thereto and stirred sufficiently uniformly.
Example 20
The coating treatment recipe for the seeds of this example was: 3% of organic macromolecular carbon sol, 8g/L of MS culture medium dry powder, 50mg/L of potassium permanganate, 160mg/L of salicylic acid, 1mM of methyl jasmonate, 5g/L of polyvinylpyrrolidone K40, 15g/L of hydroxyethyl cellulose (5000-6400 MPa.S), 15g/L of sodium carboxymethylcellulose (800-1200 MPa.S), 60g/L of diatomite and 50g/L of acid scarlet;
100mL of the solution prepared in example 3 was added with 1.6mL of a 10mg/mL salicylic acid mother solution and 1mL of a 100mM methyl jasmonate mother solution, and the mixture was stirred sufficiently and uniformly.
Example 21
This example was used for coating seeds with the following formulation: 3% of organic macromolecular carbon sol, 8g/L of MS culture medium dry powder, 50mg/L of potassium permanganate, 160mg/L of salicylic acid, 1mM of methyl jasmonate, 50mg/L of abscisic acid, 5g/L of polyvinylpyrrolidone K40, 15g/L of hydroxyethyl cellulose (5000-6400 MPa.S), 15g/L of sodium carboxymethylcellulose (800-1200 MPa.S), 60g/L of diatomite and 50g/L of acid scarlet;
100mL of the solution prepared in example 3 was added with 1.6mL of a 10mg/mL salicylic acid mother solution, 1mL of a 100mM methyl jasmonate mother solution, and 1mL of a 10mg/mL abscisic acid mother solution, and the mixture was stirred sufficiently and uniformly.
Example 22
This example was used for coating seeds with the following formulation: 2.5% of organic macromolecular carbon sol, 6g/L of MS culture medium dry powder, 40mg/L of potassium permanganate, 30mg/L of salicylic acid, 0.8mM of methyl jasmonate, 80mg/L of abscisic acid, 7g/L of polyvinylpyrrolidone K40, 20g/L of hydroxyethyl cellulose (5000-6400 MPa.S), 20g/L of sodium carboxymethyl cellulose (800-1200 MPa.S), 70g/L of kieselguhr and 60g/L of acid scarlet.
100mL of the solution prepared in example 10 was added with 0.3mL of a salicylic acid mother solution of 10mg/mL, 0.8mL of a methyl jasmonate mother solution of 100mM, and 0.8mL of an abscisic acid mother solution of 10mg/mL, and the mixture was stirred sufficiently and uniformly.
Corn seeds were coated with the seed coating compositions prepared in examples 3, 11-22 and control (without active ingredient), respectively, and the coated seeds were germinated in petri dishes at a germination temperature of 26 ℃ and a length of seedling and root and a dry matter weight were counted at 1 leaf and 1 heart (table 12). It can be seen that the seed coating treatment composition treatment significantly promoted the growth of root system, and both the dry matter accumulation amount and root-crown ratio of the root were significantly higher than those of the control. Different treatment effects are different, and the treatment effect with high hormone content is better. The formulation described in example 18 works best, as shown in figure 1.
TABLE 12 Effect of different seed coating treatment compositions on corn seedling growth development
Example 23
The seed coating treatment compositions prepared in examples 3, 13, 20 and 21 and a control (without active ingredients) are respectively coated on corn seeds, the coated seeds are sown in flowerpots filled with nutrient soil, the germination temperature is 22-26 ℃, and the dry matter weight of seedlings and roots is counted after the seedlings emerge for 25 days. It can be seen that the seed coating treatment composition treatment of the present invention significantly promoted the growth of root system, especially the lateral root amount of the surface root system was significantly increased, and both the dry matter accumulation amount and root-crown ratio of the root were significantly higher than the control, as shown in fig. 2 and table 13. The formulation described in example 14 works best (figure 2).
TABLE 13 Effect of different seed coating treatment compositions on corn seedling growth development
| Dry weight g of seedling | Dry root weight g | Root/crown ratio | |
| Control | 1.0508±0.2344 | 0.3505±0.0475 | 0.3336 |
| Example 3 | 1.1313±0.0942 | 0.4329±0.0053 | 0.3827 |
| Example 13 | 1.0196±0.1213 | 0.4084±0.0060 | 0.4005 |
| Example 20 | 0.9536±0.0474 | 0.3586±0.0208 | 0.3760 |
| Example 21 | 0.9516±0.3101 | 0.4084±0.1191 | 0.4292 |
Detailed description of example 24
The seed coating compositions formulated in specific examples 3, 21 and control (without active ingredient) were coated separately on corn seeds sown in the experimental plots of the crop science institute of the academy of agricultural sciences of china, with two densities per treatment set: 4000 plants/mu and 6000 plants/mu, and the row spacing is 40+80 cm. Each processing cell area is 36 square meters.
The dry matter weight of the aerial parts and the root system and the wound rate were measured at the flowering stage (Table 14). Therefore, the seed coating treatment composition remarkably promotes the growth of overground parts and root systems, the wound flow of the root systems is remarkably higher than that of the control, and the promotion effect under high density is more obvious.
TABLE 14 Effect of different seed coating treatment compositions on corn growth development (flowering phase)
Harvest stage test data as shown in table 15, both seed coating treatment compositions treatments significantly increased yield and the water content of the seeds at harvest was lower than the control, indicating that the seed coating treatment compositions treatment also had an effect of accelerating maturation. Yield composition analysis showed that the yield increase was mainly due to increased kernel number per ear. The wound flow was also significantly higher for both treatments at harvest than for the control, indicating that both treatments delayed the senescence of the root system, thereby promoting the accumulation of post-floral dry matter and increased yield.
TABLE 15 Effect of different seed coating treatment compositions treatment on corn yield (flowering phase)
In the current corn production, the increase of planting density has become a necessary trend of further high yield. However, in a high-density population, the contradiction between the population and the individual is prominent, the light transmittance of the population is reduced, the competition of individual resources is intensified, the growth and development of root systems are inhibited, the late premature senility is serious, the photosynthetic performance of leaves is reduced, the number of grains per spike and the weight of thousand grains are reduced, and finally the yield is reduced. At present, a great number of culture regulation technologies for delaying corn senescence have been reported. For example, the soil deep scarification technology, the large and small row planting technology and the chemical regulation and control technology have good effects on delaying the late-stage aging of the high-density planted corns and improving the yield. For example, the soil strip deep-loosening technology can delay the aging of the leaves after the flowers and increase the yield, and as also described in patent 201510656794.1, the spraying treatment of the growth regulator in the jointing or the stamina stage of the corn has the effects of lodging resistance, aging resistance and high light efficiency yield increase. However, the technologies increase the investment of field operation, and are not beneficial to improving the production efficiency of crops. The composition of the present invention effectively solves this major problem. The composition improves the root growth of the corn, adjusts the crown-root ratio of the corn, delays the later-period aging and increases the corn yield by coating the corn seeds.
The seed soaking, dressing or coating treatment is carried out by using the composition of the invention, thus not only improving the germination capacity of the seeds under various adversity stresses, but also improving the comprehensive resistance in the growth and development process, and also promoting the root growth of crops, regulating the crown-root ratio, delaying the later-stage aging and increasing the crop yield.
The seeds that can be treated by the present invention are not limited to the above exemplified wheat, corn and rice seeds, but have the same effect on other plant seeds.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable in various fields of endeavor to which the invention pertains, and further modifications may readily be made by those skilled in the art, it being understood that the invention is not limited to the details shown and described herein without departing from the general concept defined by the appended claims and their equivalents.
Claims (10)
1. Seed treatment composition, characterized in that it comprises an active ingredient comprising the following components in the following concentrations: 0.3-3.5% (V/V) of organic macromolecular carbon sol, 6-60mg/L of potassium permanganate and 2-10g/L of MS culture medium dry powder.
2. The seed treatment composition of claim 1, wherein the concentration of the organic macromolecular carbon sol is 0.5% -3% (V/V), the concentration of potassium permanganate is 10-50mg/L, and the concentration of the dry MS medium powder is 4-8 g/L.
3. The seed treatment composition of claim 2, wherein the active ingredient further comprises any one or more of salicylic acid, methyl jasmonate, and abscisic acid.
4. A seed treatment composition according to any one of claims 1 to 3, further comprising a binder, the binder being any one or more of gelatin, polyvinylpyrrolidone K40, polyvinylpyrrolidone K60, hydroxyethylcellulose and sodium carboxymethylcellulose.
5. The seed treatment composition of claim 4, wherein the binder comprises polyvinylpyrrolidone K60 at a concentration of 12 to 15g/L and a gelatin mixture at a concentration of 1.2 to 1.8 g/L.
6. Seed treatment composition according to claim 4, characterized in that the binder comprises polyvinylpyrrolidone K40 in a concentration of 2-7g/L, hydroxyethylcellulose in a concentration of 10-20g/L and sodium carboxymethylcellulose in a concentration of 10-20 g/L.
7. The seed treatment composition of claim 1, further comprising a dispersant comprising diatomaceous earth at a concentration of 30 to 60g/L and a colorant comprising acid scarlet at a concentration of 40 to 60 g/L.
8. The seed treatment composition of claim 1, wherein the composition is used in the form of a seed dip, dressing or coating.
9. The seed composition of claim 2, wherein the salicylic acid is present at a concentration of 30-160mg/L, the methyl jasmonate is present at a concentration of 0.2-1mM, and the abscisic acid is present at a concentration of 20-100 mg/L.
10. Use of the seed treatment composition of claim 1 for resisting low temperature germination and promoting root system development and delaying late senescence in plants.
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| CN115643992A (en) * | 2022-11-04 | 2023-01-31 | 中国农业科学院都市农业研究所 | Method for preventing leaf tip withering in rice rapid breeding process based on high-voltage electrostatic field |
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