CN108739401A - A kind of production method of rice paddy seed - Google Patents
A kind of production method of rice paddy seed Download PDFInfo
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- CN108739401A CN108739401A CN201810656844.XA CN201810656844A CN108739401A CN 108739401 A CN108739401 A CN 108739401A CN 201810656844 A CN201810656844 A CN 201810656844A CN 108739401 A CN108739401 A CN 108739401A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Developmental Biology & Embryology (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of production methods of rice paddy seed, include the following steps：S1, rice paddy seed are selected；The formation of S2, Rice Callus；S3, inducing paddy rice callus are sprouted：Rice Callus is gripped sizeable one section using tweezers and pliers, and it is gently put into sprouts culture medium, 25 DEG C of culture 48h；S4, culture of rootage：Rice Callus is taken out from culture medium of sprouting and is transferred in root media together with rice bud and is cultivated, it is transferred in MS solid mediums after 25 DEG C of culture 48h and continues to cultivate, be transferred to after rice root and rice bud grow plantation Tanaka plantation, to rice maturation after harvest rice paddy seed.The present invention produces more rice paddy seeds by carrying out dedifferentiation and breaking up again rice paddy seed, can not only ensure that the merit of rice paddy seed is not destroyed, and the production time of rice paddy seed is short, the productivity height of rice paddy seed.
Description
Technical field
The present invention relates to field of agricultural production technologies more particularly to a kind of production methods of rice paddy seed.
Background technology
China agricultural resource occupancy volume per person is very low, and per capita cultivated land's occupancy volume is only the one third of world average level,
With the quickening of urbanization process, the trend successively decreased year by year is presented in China's cultivated area, existing with the research and development of various hybrid rice
Rice is substantially hybrid rice in the market, and hybrid rice has disease-resistant resistance strong, and stable yields and high yield etc. are a series of
Advantage has widely been promoted the use of in China, the production carried out next time but hybrid rice cannot reserve seed for planting, therefore, I
Propose a kind of production method of rice paddy seed for solving the above problems.
Invention content
The purpose of the present invention is to solve hybrid rice exists in the prior art cannot reserve seed for planting to carry out production next time
The shortcomings that, and a kind of production method of the rice paddy seed proposed.
A kind of production method of rice paddy seed, includes the following steps：
S1, rice paddy seed are selected：Select good stress resistance, tillering ability is strong, and fringe bigger than normal, eutrophy, commodity is good, superior rice and
Suitable for rice seedling on upland field, the excellent paddy rice cross breeding seed of superthin spacing pattern；
The formation of S2, Rice Callus：Excellent paddy rice cross breeding seed is peelled off into ethyl alcohol and the matter that kind of skin mass fraction is 75%
The liquor potassic permanganate that amount score is 0.6% is dried up after sterilizing successively using sterile water wash, by the excellent hybrid rice seed of peeling
In son access inducing culture, it is placed in 27 degrees Celsius of incubators and is inverted culture, obtain Rice Callus；
S3, inducing paddy rice callus are sprouted：By Rice Callus using tweezers and sizeable one section of pliers gripping, and
It is gently put into sprouting culture medium, 25 DEG C of culture 48h；
S4, culture of rootage：Rice Callus is taken out together with rice bud from culture medium of sprouting and is transferred to training of taking root
It supports and is cultivated in base, be transferred in MS solid mediums after 25 DEG C of culture 48h and continue to cultivate, when rice root and rice bud grow
After be transferred to plantation Tanaka plantation, to rice maturation after harvest rice paddy seed.
Preferably, the constituent of the inducing culture is potassium nitrate 1.9g/L, ammonium nitrate 1.6g/L, potassium dihydrogen phosphate
0.17g/L, magnesium sulfate 0.37g/L, calcium chloride 4.4g/L, sucrose 30g/L, agar 7g/L, potassium iodide 0.83mg/L, boric acid
6.2mg/L, manganese sulfate 22.3mg/L, zinc sulfate 8.6mg/L, sodium sulphate 0.25mg/L, copper sulphate 0.025mg/L, cobalt chloride
0.025mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate 27.8mg/L, inositol 100mg/L, glycine 2mg/L,
Thiamine hydrochloride 0.1mg/L, puridoxine hydrochloride 0.5mg/L, niacin 0.5mg/L, benzyladenine 0.4mg/l, 6- Furfurylamino
Purine 0.4mg/l, auximone 2.2mg/L, abscisic acid 4.8mg/L, metakentrin 300.0mg/L, agar 2%, surplus are
Distilled water.
Preferably, the Rice Callus is that institutional framework is fine and close, smooth yellowish, the embryo that diameter is 2-3 millimeters
Property tissue particles.
Preferably, the constituent of the culture medium of sprouting is potassium nitrate 1.9g/L, ammonium nitrate 1.6g/L, potassium dihydrogen phosphate
0.17g/L, magnesium sulfate 0.37g/L, calcium chloride 4.4g/L, sucrose 30g/L, agar 7g/L, potassium iodide 0.83mg/L, boric acid
6.2mg/L, manganese sulfate 22.3mg/L, zinc sulfate 8.6mg/L, sodium sulphate 0.25mg/L, copper sulphate 0.025mg/L, cobalt chloride
0.025mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate 27.8mg/L, inositol 100mg/L, glycine 2mg/L,
Thiamine hydrochloride 0.1mg/L, puridoxine hydrochloride 0.5mg/L, niacin 0.5mg/L, benzyladenine 0.4mg/l, 6- Furfurylamino
Purine 0.4mg/l, heteroauxin 2.2mg/L, plant cytokinin 3.0mg/L, agar 2%, surplus are distilled water.
Preferably, the constituent of the root media is potassium nitrate 1.9g/L, ammonium nitrate 1.6g/L, potassium dihydrogen phosphate
0.17g/L, magnesium sulfate 0.37g/L, calcium chloride 4.4g/L, sucrose 30g/L, agar 7g/L, potassium iodide 0.83mg/L, boric acid
6.2mg/L, manganese sulfate 22.3mg/L, zinc sulfate 8.6mg/L, sodium sulphate 0.25mg/L, copper sulphate 0.025mg/L, cobalt chloride
0.025mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate 27.8mg/L, inositol 100mg/L, glycine 2mg/L,
Thiamine hydrochloride 0.1mg/L, puridoxine hydrochloride 0.5mg/L, niacin 0.5mg/L, benzyladenine 0.4mg/l, 6- Furfurylamino
Purine 0.4mg/l, heteroauxin 2.2mg/L, plant cytokinin 1.8mg/L, agar 2%, surplus are distilled water.
Preferably, being formed under no light condition for the Rice Callus carries out, and Rice Callus sprouts and takes root
Culture is carrying out under illumination condition, and intensity of illumination is 1200lx.
The beneficial effects of the invention are as follows：The present invention is by carrying out dedifferentiation by rice paddy seed and breaking up again more to produce
Rice paddy seed can not only ensure that the merit of rice paddy seed is not destroyed, and the production time of rice paddy seed is short, rice
The productivity of seed is high.
Specific implementation mode
The present invention is made further to explain with reference to specific embodiment.
Embodiment
A kind of production method of rice paddy seed, includes the following steps：
S1, rice paddy seed are selected：Select good stress resistance, tillering ability is strong, and fringe bigger than normal, eutrophy, commodity is good, superior rice and
Suitable for rice seedling on upland field, the excellent paddy rice cross breeding seed of superthin spacing pattern；
The formation of S2, Rice Callus：Excellent paddy rice cross breeding seed is peelled off into ethyl alcohol and the matter that kind of skin mass fraction is 75%
The liquor potassic permanganate that amount score is 0.6% is dried up after sterilizing successively using sterile water wash, by the excellent hybrid rice seed of peeling
In son access inducing culture（The constituent of inducing culture is potassium nitrate 1.9g/L, ammonium nitrate 1.6g/L, potassium dihydrogen phosphate
0.17g/L, magnesium sulfate 0.37g/L, calcium chloride 4.4g/L, sucrose 30g/L, agar 7g/L, potassium iodide 0.83mg/L, boric acid
6.2mg/L, manganese sulfate 22.3mg/L, zinc sulfate 8.6mg/L, sodium sulphate 0.25mg/L, copper sulphate 0.025mg/L, cobalt chloride
0.025mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate 27.8mg/L, inositol 100mg/L, glycine 2mg/L,
Thiamine hydrochloride 0.1mg/L, puridoxine hydrochloride 0.5mg/L, niacin 0.5mg/L, benzyladenine 0.4mg/l, 6- Furfurylamino
Purine 0.4mg/l, auximone 2.2mg/L, abscisic acid 4.8mg/L, metakentrin 300.0mg/L, agar 2%, surplus are
Distilled water）, it is placed in 27 degrees Celsius of incubators and is inverted culture, obtains institutional framework densification, smooth yellowish, diameter 2
The embryonal connective tissue particle of millimeter is Rice Callus；
S3, inducing paddy rice callus are sprouted：By Rice Callus using tweezers and sizeable one section of pliers gripping, and
It is gently put into sprouting culture medium（Sprout culture medium constituent be potassium nitrate 1.9g/L, ammonium nitrate 1.6g/L, phosphorus
Acid dihydride potassium 0.17g/L, magnesium sulfate 0.37g/L, calcium chloride 4.4g/L, sucrose 30g/L, agar 7g/L, potassium iodide 0.83mg/L,
Boric acid 6.2mg/L, manganese sulfate 22.3mg/L, zinc sulfate 8.6mg/L, sodium sulphate 0.25mg/L, copper sulphate 0.025mg/L, chlorination
Cobalt 0.025mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate 27.8mg/L, inositol 100mg/L, glycine 2mg/
L, thiamine hydrochloride 0.1mg/L, puridoxine hydrochloride 0.5mg/L, niacin 0.5mg/L, benzyladenine 0.4mg/l, 6- furfuryl group ammonia
Base purine 0.4mg/l, heteroauxin 2.2mg/L, plant cytokinin 3.0mg/L, agar 2%, surplus is distilled water）, 25 DEG C
Cultivate 48h；
S4, culture of rootage：Rice Callus is taken out together with rice bud from culture medium of sprouting and is transferred to training of taking root
It supports and is cultivated in base（The constituent of root media be potassium nitrate 1.9g/L, ammonium nitrate 1.6g/L, potassium dihydrogen phosphate 0.17g/L,
Magnesium sulfate 0.37g/L, calcium chloride 4.4g/L, sucrose 30g/L, agar 7g/L, potassium iodide 0.83mg/L, boric acid 6.2mg/L, sulfuric acid
Manganese 22.3mg/L, zinc sulfate 8.6mg/L, sodium sulphate 0.25mg/L, copper sulphate 0.025mg/L, cobalt chloride 0.025mg/L, second two
Amine tetraacethyl disodium 37.3mg/L, ferrous sulfate 27.8mg/L, inositol 100mg/L, glycine 2mg/L, thiamine hydrochloride
0.1mg/L, puridoxine hydrochloride 0.5mg/L, niacin 0.5mg/L, benzyladenine 0.4mg/l, 6-nonylaminopurine 0.4mg/
L, heteroauxin 2.2mg/L, plant cytokinin 1.8mg/L, agar 2%, surplus are distilled water）, 25 DEG C culture 48h after turn
It moves on in MS solid mediums and continues to cultivate, plantation Tanaka's plantation is transferred to after rice root and rice bud grow, arrives water
Rice paddy seed is harvested after rice maturation, being formed under no light condition for Rice Callus carries out, and Rice Callus is sprouted and given birth to
Root culture is carrying out under illumination condition, and intensity of illumination is 1200lx.
Comparative example
S3 and S4 steps transposition, other conditions in embodiment are constant.
The rice paddy seed of 200 the same terms will be taken, is equally divided into two parts, a copy of it is tried in way of example
It tests, another is tested in the method for comparative example, observes the planting percent of rice seedling in two groups of experiments, and test result is as follows：
| Embodiment | Comparative example | |
| Planting percent（%） | 98 | 1.6 |
By above-mentioned experimental result it is found that in a kind of production method of rice paddy seed proposed by the present invention rice paddy seed planting percent
It is high.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Any one skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (6)
1. a kind of production method of rice paddy seed, which is characterized in that include the following steps：
S1, rice paddy seed are selected：Select good stress resistance, tillering ability is strong, and fringe bigger than normal, eutrophy, commodity is good, superior rice and
Suitable for rice seedling on upland field, the excellent paddy rice cross breeding seed of superthin spacing pattern；
The formation of S2, Rice Callus：Excellent paddy rice cross breeding seed is peelled off into ethyl alcohol and the matter that kind of skin mass fraction is 75%
The liquor potassic permanganate that amount score is 0.6% is dried up after sterilizing successively using sterile water wash, by the excellent hybrid rice seed of peeling
In son access inducing culture, it is placed in 27 degrees Celsius of incubators and is inverted culture, obtain Rice Callus；
S3, inducing paddy rice callus are sprouted：By Rice Callus using tweezers and sizeable one section of pliers gripping, and
It is gently put into sprouting culture medium, 25 DEG C of culture 48h；
S4, culture of rootage：Rice Callus is taken out together with rice bud from culture medium of sprouting and is transferred to training of taking root
It supports and is cultivated in base, be transferred in MS solid mediums after 25 DEG C of culture 48h and continue to cultivate, when rice root and rice bud grow
After be transferred to plantation Tanaka plantation, to rice maturation after harvest rice paddy seed.
2. a kind of production method of rice paddy seed according to claim 1, which is characterized in that the group of the inducing culture
It is potassium nitrate 1.9g/L, ammonium nitrate 1.6g/L, potassium dihydrogen phosphate 0.17g/L, magnesium sulfate 0.37g/L, calcium chloride 4.4g/ at ingredient
L, sucrose 30g/L, agar 7g/L, potassium iodide 0.83mg/L, boric acid 6.2mg/L, manganese sulfate 22.3mg/L, zinc sulfate 8.6mg/L,
Sodium sulphate 0.25mg/L, copper sulphate 0.025mg/L, cobalt chloride 0.025mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, sulfuric acid
Ferrous 27.8mg/L, inositol 100mg/L, glycine 2mg/L, thiamine hydrochloride 0.1mg/L, puridoxine hydrochloride 0.5mg/L, niacin
0.5mg/L, benzyladenine 0.4mg/l, 6-nonylaminopurine 0.4mg/l, auximone 2.2mg/L, abscisic acid
4.8mg/L, metakentrin 300.0mg/L, agar 2%, surplus are distilled water.
3. a kind of production method of rice paddy seed according to claim 1, which is characterized in that the Rice Callus is
Institutional framework is fine and close, smooth yellowish, the embryonal connective tissue particle that diameter is 2-3 millimeters.
4. a kind of production method of rice paddy seed according to claim 1, which is characterized in that the group of the culture medium of sprouting
It is potassium nitrate 1.9g/L, ammonium nitrate 1.6g/L, potassium dihydrogen phosphate 0.17g/L, magnesium sulfate 0.37g/L, calcium chloride 4.4g/ at ingredient
L, sucrose 30g/L, agar 7g/L, potassium iodide 0.83mg/L, boric acid 6.2mg/L, manganese sulfate 22.3mg/L, zinc sulfate 8.6mg/L,
Sodium sulphate 0.25mg/L, copper sulphate 0.025mg/L, cobalt chloride 0.025mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, sulfuric acid
Ferrous 27.8mg/L, inositol 100mg/L, glycine 2mg/L, thiamine hydrochloride 0.1mg/L, puridoxine hydrochloride 0.5mg/L, niacin
0.5mg/L, benzyladenine 0.4mg/l, 6-nonylaminopurine 0.4mg/l, heteroauxin 2.2mg/L, plant cell division
Plain 3.0mg/L, agar 2%, surplus are distilled water.
5. a kind of production method of rice paddy seed according to claim 1, which is characterized in that the group of the root media
It is potassium nitrate 1.9g/L, ammonium nitrate 1.6g/L, potassium dihydrogen phosphate 0.17g/L, magnesium sulfate 0.37g/L, calcium chloride 4.4g/ at ingredient
L, sucrose 30g/L, agar 7g/L, potassium iodide 0.83mg/L, boric acid 6.2mg/L, manganese sulfate 22.3mg/L, zinc sulfate 8.6mg/L,
Sodium sulphate 0.25mg/L, copper sulphate 0.025mg/L, cobalt chloride 0.025mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, sulfuric acid
Ferrous 27.8mg/L, inositol 100mg/L, glycine 2mg/L, thiamine hydrochloride 0.1mg/L, puridoxine hydrochloride 0.5mg/L, niacin
0.5mg/L, benzyladenine 0.4mg/l, 6-nonylaminopurine 0.4mg/l, heteroauxin 2.2mg/L, plant cell division
Plain 1.8mg/L, agar 2%, surplus are distilled water.
6. a kind of production method of rice paddy seed according to claim 1, which is characterized in that the Rice Callus
It is formed under no light condition and carries out, Rice Callus, which is sprouted, to carry out with culture of rootage under illumination condition, and intensity of illumination
For 1200lx.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810656844.XA CN108739401A (en) | 2018-06-24 | 2018-06-24 | A kind of production method of rice paddy seed |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810656844.XA CN108739401A (en) | 2018-06-24 | 2018-06-24 | A kind of production method of rice paddy seed |
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| CN108739401A true CN108739401A (en) | 2018-11-06 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110169357A (en) * | 2019-06-18 | 2019-08-27 | 天津市农作物研究所(天津市水稻研究所) | A kind of production method of rice paddy seed |
| CN111165494A (en) * | 2020-01-21 | 2020-05-19 | 中国农业科学院作物科学研究所 | Seed treatment composition and use |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03119937A (en) * | 1989-09-30 | 1991-05-22 | Naasarii Technol:Kk | Method for developing seedling from rice plant adventitious embryo |
| CN1711831A (en) * | 2004-06-24 | 2005-12-28 | 华中农业大学 | Cut culture of indica rice |
| US20100192253A1 (en) * | 2009-01-26 | 2010-07-29 | Pioneer Hi-Bred, Inc. | Methods for improving monocot transformation |
| CN108148799A (en) * | 2017-12-30 | 2018-06-12 | 青岛袁策生物科技有限公司 | The method of high frequency zone Rice Callus culture medium |
-
2018
- 2018-06-24 CN CN201810656844.XA patent/CN108739401A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03119937A (en) * | 1989-09-30 | 1991-05-22 | Naasarii Technol:Kk | Method for developing seedling from rice plant adventitious embryo |
| CN1711831A (en) * | 2004-06-24 | 2005-12-28 | 华中农业大学 | Cut culture of indica rice |
| US20100192253A1 (en) * | 2009-01-26 | 2010-07-29 | Pioneer Hi-Bred, Inc. | Methods for improving monocot transformation |
| CN108148799A (en) * | 2017-12-30 | 2018-06-12 | 青岛袁策生物科技有限公司 | The method of high frequency zone Rice Callus culture medium |
Non-Patent Citations (5)
| Title |
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| 北京医学院: "《生物化学》", 31 December 1978, 人民卫生出版社 * |
| 毛丹 等: "《农作物科学用药手册》", 28 February 2018, 中原农民出版社 * |
| 谢静萱: "杂交水稻快速无性繁殖", 《植物生理学通讯》 * |
| 过全生 等: "低浓度2, 4-D提高水稻体细胞成苗研究", 《中国水稻科学》 * |
| 邢小黑: "水稻离体培养及不定芽分化", 《核农学通报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110169357A (en) * | 2019-06-18 | 2019-08-27 | 天津市农作物研究所(天津市水稻研究所) | A kind of production method of rice paddy seed |
| CN111165494A (en) * | 2020-01-21 | 2020-05-19 | 中国农业科学院作物科学研究所 | Seed treatment composition and use |
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Application publication date: 20181106 |