CN111165238A - Method for preventing and treating tree diseases by using microorganisms - Google Patents
Method for preventing and treating tree diseases by using microorganisms Download PDFInfo
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- CN111165238A CN111165238A CN202010202381.7A CN202010202381A CN111165238A CN 111165238 A CN111165238 A CN 111165238A CN 202010202381 A CN202010202381 A CN 202010202381A CN 111165238 A CN111165238 A CN 111165238A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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Abstract
The invention relates to the technical field of forest control, in particular to a method for controlling tree diseases by using microorganisms, which comprises the following steps: s1, soil treatment: mixing trichoderma powder and seedling culture medium in a ratio of 1: the mixture of 500 is fully mixed and then is directly sown or cut; if the seedbed is directly treated, the ratio of 1: 10 and the seedling raising matrix are uniformly mixed, and then the mixture is scattered into a seedbed, wherein each kilogram of seedbed is treated by 10-15 square meters; s2, seed dressing or seed soaking: mixing trichoderma agent with a dilution matrix according to the ratio of 1: the wood tree pesticide is diluted by 100 times, the designed microorganism prevents and treats tree diseases by diluting and proportioning trichoderma and EM stock solution, the influence of the existing chemical reagent on the environment is solved, the problem that the pesticide is used on fruit trees, the existing residues are difficult to completely remove and cause great harm to human bodies due to the fact that the pesticide is a toxic chemical reagent is solved, the survival rate of tree seedlings is improved, the development of forestry in China is promoted, and the residues of the chemical reagent are prevented from causing harm to the human bodies.
Description
Technical Field
The invention relates to the technical field of forest control, in particular to a method for controlling tree diseases by using microorganisms.
Background
In recent years, the seedling pest and disease damage rate of tree species of forestry in China is increased year by year, the development of the forestry in China is limited to a great extent, so that the survival rate of seedlings of the tree species of the forestry is reduced, the situation is extremely unfavorable for the development of the environmental protection industry in China, so that the pest damage of the forestry is reduced.
In summary, the present invention solves the existing problems by designing a method for preventing and treating tree diseases by using microorganisms.
Disclosure of Invention
The present invention is directed to a method for preventing and treating tree diseases by using microorganisms, which solves the above problems of the prior art.
In order to achieve the purpose, the invention provides the following technical scheme:
a method for controlling tree diseases by using microorganisms comprises the following steps:
s1, soil treatment: mixing trichoderma powder and seedling culture medium in a ratio of 1: the mixture of 500 is fully mixed and then is directly sown or cut; if the seedbed is directly treated, the ratio of 1: 10 and the seedling raising matrix are uniformly mixed, and then the mixture is scattered into a seedbed, wherein each kilogram of seedbed is treated by 10-15 square meters;
s2, seed dressing or seed soaking: mixing trichoderma agent with a dilution matrix according to the ratio of 1: diluting by a proportion of 100 times to obtain trichoderma agent diluent A, and soaking the roots of the forest for 2 hours and then sowing, or mixing the seeds and then cutting;
s3, root processing: before transplanting, mixing trichoderma agent and dilution matrix according to the ratio of 1: diluting by a proportion of 500 times to obtain trichoderma agent diluent B, soaking the roots for 30 minutes, and then setting a value; after transplanting, mixing trichoderma agent and dilution matrix according to the ratio of 1: diluting by a proportion of 500 times to obtain trichoderma agent diluent C for root irrigation;
s4, spraying trichoderma agent: transplanting the forest tree seedlings for 3-4 days, and mixing trichoderma agent and dilution matrix according to the weight ratio of 1: diluting by 600-800 times to obtain trichoderma agent diluent D, and spraying the trichoderma agent diluent D by a sprayer;
s5, spraying the microbial insect-resist solution; spraying the microbial insect-resistant liquid according to the ratio of 1: diluting at a ratio of 500-1000 times, spraying in mist form, and spraying on both sides of the leaf.
Preferably, the seedling raising substrate in the S1 is moist soil in a farmland, and the dilution substrates of S2, S3, S4 and S5 are tap water.
Preferably, the preparation method of the microbial insect-resistant solution in S5 comprises the following specific steps:
s1, selecting the following raw materials: EM stock solution, molasses, vinegar, white spirit and clear water;
s2, firstly putting the EM stock solution and molasses into a stirrer and continuously stirring and uniformly stirring, secondly filling into a glass container and tightly covering, fermenting for 2-3 days, after gas generation and swelling, finally uncovering and deflating, adding vinegar and white spirit which are equal to the EM stock solution, uniformly stirring and tightly covering, and fermenting for about 2-3 weeks, wherein if gas generation and the container swelling occur in the meantime, timely deflating and immediately tightly covering are needed, and when the gas is not generated any more and the sweet and sour ester fragrance can be smelled, the successful fermentation is indicated.
Preferably, the EM stock solution, the molasses, the vinegar, the white spirit and the clear water in the S1 are prepared according to the ratio of 1: 10.
Preferably, the EM stock solution in the S1 is bacillus subtilis, bacillus licheniformis, bacillus thuringiensis, photosynthetic bacteria, lactic acid bacteria, yeast acid, actinomycetes and a small amount of culture medium dry substances, and the total bacteria number is more than or equal to 200 hundred million/g.
Preferably, the degree of the white spirit in the S1 is 30-35 degrees.
Compared with the prior art, the invention has the beneficial effects that:
1. in the invention, through utilizing dilution and proportion of trichoderma and EM stock solution, the designed microorganism prevents and treats tree diseases, thereby solving the influence of the existing chemical reagent on the environment, solving the problem that the residue is difficult to completely remove and causes great harm to human body because the pesticide is a toxic chemical reagent when being used on fruit trees, further improving the survival rate of saplings, promoting the development of forestry in China, and avoiding the residue of the chemical reagent from causing harm to human body, wherein the microorganism insect-preventing solution enhances the metabolism of plants, strengthens the cuticle of a leaf protecting film, prevents the entry of pathogenic bacteria, does not decompose ester components in bodies of herbivorous pests, causes physiological disturbance and death, has obvious effect on various pests such as nematodes and the like, and the trichoderma can generate a plurality of bioactive substances with antagonistic action on plant pathogenic fungi, bacteria and insects, thereby achieving the purposes of preventing diseases, The fertilizer has the effects of treating diseases, promoting growth, improving nutrient utilization rate, enhancing crop stress resistance, repairing agricultural and chemical environmental pollution and the like.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
The invention provides a technical scheme that:
a method for controlling tree diseases by using microorganisms comprises the following steps:
s1, soil treatment: mixing trichoderma powder and seedling culture medium in a ratio of 1: the mixture of 500 is fully mixed and then is directly sown or cut; if the seedbed is directly treated, the ratio of 1: 10 and the seedling raising matrix are uniformly mixed, and then the mixture is scattered into a seedbed, wherein each kilogram of seedbed is treated by 10-15 square meters;
s2, seed dressing or seed soaking: mixing trichoderma agent with a dilution matrix according to the ratio of 1: diluting by a proportion of 100 times to obtain trichoderma agent diluent A, and soaking the roots of the forest for 2 hours and then sowing, or mixing the seeds and then cutting;
s3, root processing: before transplanting, mixing trichoderma agent and dilution matrix according to the ratio of 1: diluting by a proportion of 500 times to obtain trichoderma agent diluent B, soaking the roots for 30 minutes, and then setting a value; after transplanting, mixing trichoderma agent and dilution matrix according to the ratio of 1: diluting by a proportion of 500 times to obtain trichoderma agent diluent C for root irrigation;
s4, spraying trichoderma agent: transplanting the forest tree seedlings for 3-4 days, and mixing trichoderma agent and dilution matrix according to the weight ratio of 1: diluting by 600-800 times to obtain trichoderma agent diluent D, and spraying the trichoderma agent diluent D by a sprayer;
s5, spraying the microbial insect-resist solution; spraying the microbial insect-resistant liquid according to the ratio of 1: diluting at a ratio of 500-1000 times, spraying in mist form, and spraying on both sides of the leaf.
The specific implementation case is as follows:
selecting three groups of forest nursery gardens which are respectively a control group, a chemical group and a microorganism group, and respectively planting 30 same kinds of forest seedlings according to the row spacing and the column spacing of 30cm and 30 cm;
wherein, 30 seedlings of the trees in the control group are planted according to a common planting method, and no insecticidal chemical reagent or chemical reagent for promoting the growth of the trees is applied;
planting 30 tree seedlings in the chemical group according to a common planting method, and applying an insecticidal chemical reagent and a chemical reagent for promoting the growth of trees;
30 forest seedlings in the microbial group were treated according to the following steps:
step 1, soil treatment: mixing trichoderma powder and seedling culture medium in a ratio of 1: the mixture of 500 is fully mixed and then is directly sown or cut; if the seedbed is directly treated, the ratio of 1: 10 and the seedling raising matrix are uniformly mixed, and then the mixture is scattered into a seedbed, wherein each kilogram of seedbed is treated by 10-15 square meters;
step 2, seed dressing or seed soaking: mixing trichoderma agent with a dilution matrix according to the ratio of 1: diluting by a proportion of 100 times to obtain trichoderma agent diluent A, and soaking the roots of the forest for 2 hours and then sowing, or mixing the seeds and then cutting;
step 3, root treatment: before transplanting, mixing trichoderma agent and dilution matrix according to the ratio of 1: diluting by a proportion of 500 times to obtain trichoderma agent diluent B, soaking the roots for 30 minutes, and then setting a value; after transplanting, mixing trichoderma agent and dilution matrix according to the ratio of 1: diluting by a proportion of 500 times to obtain trichoderma agent diluent C for root irrigation;
step 4, spraying of trichoderma agent: transplanting the forest tree seedlings for 3-4 days, and mixing trichoderma agent and dilution matrix according to the weight ratio of 1: diluting by 600-800 times to obtain trichoderma agent diluent D, and spraying the trichoderma agent diluent D by a sprayer;
step 5, spraying the microbial insect-proof liquid; spraying the microbial insect-resistant liquid according to the ratio of 1: diluting at a ratio of 500-1000 times, spraying in mist form, and spraying on both sides of the leaf.
After 1-2 months, the survival rates of 30 seedlings in the chemical group and the microbial group are clearly observed to be nearly the same, the survival rates of 30 seedlings in the chemical group and the microbial group are observed to be far higher than the survival rates of 30 seedlings in the control group, a chromatograph analyzes chemical components in the 30 seedlings in the chemical group and the microbial group respectively, and after analysis, components of insecticidal chemical reagents and chemical reagents for promoting the growth of trees in the 30 seedlings in the chemical group are analyzed, in conclusion, in the scheme, the designed microorganisms are used for preventing and treating the trees by utilizing the dilution and the proportion of trichoderma and EM stock solution, so that the influence of the existing chemical reagents on the environment is solved, the problem that the existing residues are difficult to completely remove and cause great harm to human bodies due to the fact that the insecticides are toxic chemical reagents when the chemical reagents are used on fruit trees is solved, thereby improving the survival rate of the saplings, promoting the development of forestry in China and avoiding the damage of the residues of chemical reagents to human bodies.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (6)
1. A method for controlling tree diseases by using microorganisms comprises the following steps:
s1, soil treatment: mixing trichoderma powder and seedling culture medium in a ratio of 1: the mixture of 500 is fully mixed and then is directly sown or cut; if the seedbed is directly treated, the ratio of 1: 10 and the seedling raising matrix are uniformly mixed, and then the mixture is scattered into a seedbed, wherein each kilogram of seedbed is treated by 10-15 square meters;
s2, seed dressing or seed soaking: mixing trichoderma agent with a dilution matrix according to the ratio of 1: diluting by a proportion of 100 times to obtain trichoderma agent diluent A, and soaking the roots of the forest for 2 hours and then sowing, or mixing the seeds and then cutting;
s3, root processing: before transplanting, mixing trichoderma agent and dilution matrix according to the ratio of 1: diluting by a proportion of 500 times to obtain trichoderma agent diluent B, soaking the roots for 30 minutes, and then setting a value; after transplanting, mixing trichoderma agent and dilution matrix according to the ratio of 1: diluting by a proportion of 500 times to obtain trichoderma agent diluent C for root irrigation;
s4, spraying trichoderma agent: transplanting the forest tree seedlings for 3-4 days, and mixing trichoderma agent and dilution matrix according to the weight ratio of 1: diluting by 600-800 times to obtain trichoderma agent diluent D, and spraying the trichoderma agent diluent D by a sprayer;
s5, spraying the microbial insect-resist solution; spraying the microbial insect-resistant liquid according to the ratio of 1: diluting at a ratio of 500-1000 times, spraying in mist form, and spraying on both sides of the leaf.
2. The method for preventing and treating tree diseases by using microorganisms as claimed in claim 1, wherein the seedling raising substrate in S1 is moist soil in farmlands, and the diluted substrates S2, S3, S4 and S5 are tap water.
3. The method for preventing and treating tree diseases by using microorganisms as claimed in claim 1, wherein the microbial insect repellent solution in S5 is prepared by the following steps:
s1, selecting the following raw materials: EM stock solution, molasses, vinegar and white spirit;
s2, firstly putting the EM stock solution and molasses into a stirrer and continuously stirring and uniformly stirring, secondly filling into a glass container and tightly covering, fermenting for 2-3 days, after gas generation and swelling, finally uncovering and deflating, adding vinegar and white spirit which are equal to the EM stock solution, uniformly stirring and tightly covering, and fermenting for about 2-3 weeks, wherein if gas generation and the container swelling occur in the meantime, timely deflating and immediately tightly covering are needed, and when the gas is not generated any more and the sweet and sour ester fragrance can be smelled, the successful fermentation is indicated.
4. The method of claim 3, wherein the EM stock solution, molasses, vinegar, white spirit and clear water in the S1 are prepared in a ratio of 1: 10.
5. The method of claim 3, wherein the EM stock solution in S1 is selected from the group consisting of Bacillus subtilis, Bacillus licheniformis, Bacillus thuringiensis, photosynthetic bacteria, lactic acid bacteria, yeast acid, actinomycetes, and a small amount of a dry matter of a culture medium, and the total number of bacteria is not less than 200 hundred million/g.
6. The method for preventing and treating tree diseases by using microorganisms as claimed in claim 1, wherein the degree of white spirit in S1 is 30-35 degrees.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010202381.7A CN111165238A (en) | 2020-03-20 | 2020-03-20 | Method for preventing and treating tree diseases by using microorganisms |
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| CN202010202381.7A CN111165238A (en) | 2020-03-20 | 2020-03-20 | Method for preventing and treating tree diseases by using microorganisms |
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Citations (5)
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Application publication date: 20200519 |