CN111150757A - Method for preventing influenza in poultry breeding in animal husbandry - Google Patents
Method for preventing influenza in poultry breeding in animal husbandry Download PDFInfo
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Abstract
The invention discloses a method for preventing influenza in poultry raising in animal husbandry, which comprises the following materials in parts by weight: 80-100 parts of bat dung, 10-20 parts of solidago decurrens, 10-20 parts of liquorice, 1-15 parts of mulberry leaves, 1-10 parts of honeysuckle, 3-13 parts of isatis roots, 1-10 parts of platycodon grandiflorum powder, 10-50 parts of alginic acid, 20-60 parts of calcium lactate and the balance of distilled liquor, the method has simple steps and convenient operation, has better prevention and protection effects on avian influenza of chickens in animal husbandry, has no residue in chickens and is not easy to generate drug-resistant strains; the raw materials have low cost, safety and effectiveness, and no toxicity; the additive is added into the daily chicken feed according to the weight ratio of 1 percent, and can also effectively reduce the excretion of nitrogen and phosphorus in chicken manure.
Description
Technical Field
The invention belongs to the technical field of epidemic prevention of animal husbandry, and particularly relates to an influenza prevention medicine for the animal husbandry chicken breeding and a preparation method thereof, and more particularly relates to an influenza prevention method for the animal husbandry chicken breeding.
Background
Avian influenza (H5N1) is a widespread infectious disease that can be transmitted by migratory birds with a real potential threat to human health and to the poultry industry. However, human beings have not found a better control method except 'one-time killing', and since 2003, the highly pathogenic H5N1 avian influenza is almost hurricane-type and rolls over the poultry breeding industry of Asian countries, so that the loss is heavy and the disaster continues. Highly pathogenic avian influenza viruses are prevalent in poultry throughout the world and are ubiquitous in some countries. The mortality of poultry caused by this virus is extremely high. Further, when the infection with the virus is confirmed in japan, all the surrounding poultry are sacrificed for epidemic prevention. Therefore, the economic loss of the poultry industry caused by this virus is enormous, and there is a strong need for a truly effective and rational method of prevention.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a drug for preventing influenza in poultry farming and a preparation method thereof.
In order to achieve the purpose, the invention provides the following technical scheme: the drug for preventing the influenza in the poultry raising comprises the following materials in parts by weight: 80-100 parts of bat dung, 10-20 parts of solidago decurrens, 10-20 parts of liquorice, 1-15 parts of mulberry leaves, 1-10 parts of honeysuckle, 3-13 parts of isatis roots, 1-10 parts of platycodon grandiflorum powder, 10-50 parts of alginic acid, 20-60 parts of calcium lactate and the balance of distilled liquor.
Preferably, the medicine is prepared from the following materials in parts by weight: 90 parts of bat dung, 15 parts of goldenrod herb, 15 parts of liquorice, 8 parts of mulberry leaf, 5 parts of honeysuckle, 8 parts of isatis root, 5 parts of platycodon root powder, 30 parts of alginic acid, 40 parts of calcium lactate and 1000 parts of distilled liquor.
Preferably, the distilled spirit is kaoliang spirit with the alcohol concentration of 25-60% vol.
The invention provides a preparation method of a drug for preventing influenza in poultry raising in animal husbandry, which comprises the following steps:
s1, weighing the traditional Chinese medicinal materials, cleaning the goldenrod, liquorice, mulberry leaves, honeysuckle, isatis root and platycodon root powder, removing impurities, slicing, drying, crushing by a ball mill crusher, sieving by a sieve of 20-40 meshes, mixing and crushing into coarse powder for later use;
s2, sterilizing the container: disinfecting all container surfaces with a disinfectant, carrying out overall irradiation through ultraviolet rays for 10-300 minutes, and finally cleaning the containers with distilled water;
s3, feeding: grinding the coarse powder into fine powder by using a superfine grinder, adding 25-60% vol sorghum liquor, alginic acid and calcium lactate which are 5 times of the weight of the fine powder of the night vision sand into the fine powder of the night vision sand, heating to 46-48 ℃, putting into a sterilized container, leaching for 32-36 hours under the action of extraction pressure difference formed by the work of a high-pressure rotor pump, taking supernatant, recovering ethanol under negative pressure, and concentrating to obtain paste with the solid content of 26-42%;
s4, sterilizing the paste under 0.15-0.18Mpa for 30min, inoculating a powdery Mucor rouxii starter with the weight of 0.05-0.08% of the paste;
s5, sealing: sealing the container, standing in a cool and dry place, and fermenting at 30 deg.C for 7 days;
and S6, finally, obtaining fermented paste, drying and grinding the fermented paste to obtain the medicine for preventing the avian influenza, and storing the filled and sealed medicine at 15-20 ℃ for storage.
Preferably, the rotating speed of the ball milling grinder is 500-700 r/min, and the bottom end of a discharge hole at the bottom of the ball milling grinder is movably connected with a fine-hole filter screen with a 20-50-mesh sieve.
Preferably, the relative humidity of the ultraviolet irradiation environment in the S2 is 50-60%, the temperature is 15-20 ℃, and the ultraviolet intensity is not lower than 70uW/cm 2.
Preferably, the high-pressure rotor pump in S3 works to generate a vacuum degree of-0.092 to-0.095 MPa, and a discharge pressure of 0.2 to 0.4 MPa.
Preferably, the fermented paste is dried for 12 hours under the vacuum degree of 0.03-0.035 MPa and the temperature of 68 ℃ and then ground, so that the medicine for preventing avian influenza is obtained.
A method for preventing influenza in chicken fed in animal husbandry comprises mixing fine powder of influenza preventing medicine with feed for administration.
The invention has the technical effects and advantages that: compared with the traditional preparation process, the method for preventing the influenza of the livestock-raising chickens has the advantages that the steps are simple, the operation is convenient, the veterinary drug for preventing the avian influenza has better prevention and protection effects on the avian influenza of the chickens, no residue exists in the chickens, and drug-resistant strains are not easy to generate; the raw materials have low cost, safety and effectiveness, and no toxicity; the additive is added into the daily chicken feed according to the weight ratio of 1 percent, and can also effectively reduce the excretion of nitrogen and phosphorus in chicken manure.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The drug for preventing the influenza in the poultry raising comprises the following materials in parts by weight: 80 parts of bat dung, 10 parts of solidago decurrens, 10 parts of liquorice, 1 part of mulberry leaf, 1 part of honeysuckle, 3 parts of isatis root, 1 part of platycodon root powder, 10 parts of alginic acid, 20 parts of calcium lactate and the balance of 25% vol distilled liquor.
The invention provides a preparation method of a drug for preventing influenza in poultry raising in animal husbandry, which comprises the following steps:
s1, weighing the traditional Chinese medicinal materials, cleaning the goldenrod, liquorice, mulberry leaves, honeysuckle, isatis root and platycodon root powder, removing impurities, slicing, drying, crushing by a ball mill crusher, sieving by a 20-mesh sieve, mixing and crushing into coarse powder for later use;
s2, sterilizing the container: disinfecting all container surfaces with a disinfectant, carrying out overall irradiation through ultraviolet rays for 10 minutes, and finally cleaning the containers with distilled water;
s3, feeding: grinding the coarse powder into fine powder by using a superfine grinder, adding 25 wt% vol kaoliang spirit, alginic acid and calcium lactate which are 5 times of the weight of the fine powder of the night vision sand into the fine powder of the night vision sand, heating to 46 ℃, putting the mixture into a sterilized container, leaching for 32 hours under the action of extraction pressure difference formed by the work of a high-pressure rotor pump, taking supernatant, recovering ethanol under negative pressure, and concentrating the supernatant into paste with the solid content of 26%;
s4, sterilizing the paste under 0.15Mpa for 30min, inoculating a powdery Mucor rouxii starter with the weight of 0.05% of the paste;
s5, sealing: sealing the container, standing in a cool and dry place, and fermenting at 30 deg.C for 7 days;
and S6, finally, obtaining fermented paste, drying and grinding the fermented paste to obtain the medicine for preventing the avian influenza, and storing the filled and sealed medicine at 15 ℃ for storage.
A method for preventing influenza in chicken fed in animal husbandry comprises mixing fine powder of influenza preventing medicine with feed for administration.
Example 2
The drug for preventing the influenza in the poultry raising comprises the following materials in parts by weight: 90 parts of bat dung, 15 parts of solidago decurrens, 15 parts of liquorice, 8 parts of mulberry leaves, 5 parts of honeysuckle, 8 parts of isatis roots, 5 parts of platycodon grandiflorum powder, 30 parts of alginic acid, 40 parts of calcium lactate and the balance of 42% vol distilled liquor.
The invention provides a preparation method of a drug for preventing influenza in poultry raising in animal husbandry, which comprises the following steps:
s1, weighing the traditional Chinese medicinal materials, cleaning the goldenrod, liquorice, mulberry leaves, honeysuckle, isatis root and platycodon root powder, removing impurities, slicing, drying, crushing by a ball mill crusher, sieving by a 30-mesh sieve, mixing and crushing into coarse powder for later use;
s2, sterilizing the container: disinfecting all container surfaces with a disinfectant, carrying out overall irradiation through ultraviolet rays for 150 minutes, and finally cleaning the containers with distilled water;
s3, feeding: grinding the coarse powder into fine powder by using a superfine grinder, adding 42 wt% vol kaoliang spirit, alginic acid and calcium lactate which are 5 times of the weight of the fine powder of the night vision sand into the fine powder of the night vision sand, heating to 47 ℃, putting the mixture into a sterilized container, leaching for 34 hours under the action of extraction pressure difference formed by the work of a high-pressure rotor pump, taking supernatant, recovering ethanol under negative pressure, and concentrating the supernatant into paste with the solid content of 35%;
s4, sterilizing the paste under 0.165Mpa for 30min, inoculating a powdery Mucor rouxii starter with the weight of 0.065% of the paste;
s5, sealing: sealing the container, standing in a cool and dry place, and fermenting at 30 deg.C for 7 days;
and S6, finally, obtaining fermented paste, drying and grinding the fermented paste to obtain the medicine for preventing the avian influenza, and storing the filled and sealed medicine at 17 ℃ for storage.
A method for preventing influenza in chicken fed in animal husbandry comprises mixing fine powder of influenza preventing medicine with feed for administration.
Example 3
The drug for preventing the influenza in the poultry raising comprises the following materials in parts by weight: 100 parts of bat dung, 20 parts of solidago decurrens, 20 parts of liquorice, 15 parts of mulberry leaves, 10 parts of honeysuckle, 3-13 parts of isatis roots, 10 parts of platycodon grandiflorum powder, 50 parts of alginic acid, 60 parts of calcium lactate and the balance of 60 vol% distilled liquor.
The invention provides a preparation method of a drug for preventing influenza in poultry raising in animal husbandry, which comprises the following steps:
s1, weighing the traditional Chinese medicinal materials, cleaning the goldenrod, liquorice, mulberry leaves, honeysuckle, isatis root and platycodon root powder, removing impurities, slicing, drying, crushing by a ball mill crusher, sieving by a 40-mesh sieve, mixing and crushing into coarse powder for later use;
s2, sterilizing the container: disinfecting all container surfaces with a disinfectant, carrying out overall irradiation through ultraviolet rays for 300 minutes, and finally cleaning the container with distilled water;
s3, feeding: grinding the coarse powder into fine powder by using a superfine grinder, adding 60% vol kaoliang spirit, alginic acid and calcium lactate which are 5 times of the weight of the fine powder of the night vision sand into the fine powder of the night vision sand, heating to 48 ℃, putting the mixture into a sterilized container, leaching for 36 hours under the action of extraction pressure difference formed by the work of a high-pressure rotor pump, taking supernatant, recovering ethanol under negative pressure, and concentrating the supernatant into paste with the solid content of 42%;
s4, sterilizing the paste under 0.18Mpa for 30min, inoculating a powdery Mucor rouxii starter with the weight of 0.08% of the paste;
s5, sealing: sealing the container, standing in a cool and dry place, and fermenting at 30 deg.C for 7 days;
and S6, finally, obtaining fermented paste, drying and grinding the fermented paste to obtain the medicine for preventing the avian influenza, and storing the filled and sealed medicine at 20 ℃ for storage.
A method for preventing influenza in chicken fed in animal husbandry comprises mixing fine powder of influenza preventing medicine with feed for administration.
The invention is illustrated below with reference to specific comparative tests:
test 1, materials: strain: H5N1 avian influenza Virus (A/Goose/GD/2/96), supplied by the laboratory of the animal science institute of university of agriculture, China;
test chicken: purchasing 600 healthy chickens which are not inoculated with the avian influenza medicines at 1 day old from a chicken farm, artificially feeding the chickens to 15 days old, randomly selecting 500 chickens as test chickens, dividing the test chickens into A, B, C, D, E5 groups, wherein each group contains 100 chickens, cold boiled water is used for freely drinking water, and the basic feed is full-value feed without any antibiotic and anti-avian influenza medicines;
3 days before the challenge, the veterinary drug for preventing avian influenza obtained in example 3 is artificially fed to the group A, 3 times a day, and the group A is fed according to 50 mg/kg;
feeding group B with paste containing 42% of solid obtained in example 3, drying at 68 deg.C under vacuum degree of 0.035MPa for 12 hr, grinding to obtain medicine, and feeding group B at 50mg/kg for 3 times per day;
feeding the mixture into group C with faeces Vespertilionis fine powder 3 times per day, and feeding group C with 50mg/kg feed;
group D is a control group for counteracting toxic substance, and no drug is administered for counteracting toxic substance;
group E was a blank control group, no drug administration, no toxicity.
The chickens in each group are separately fed, A, B, C, D four groups of chickens are injected with 10-4 multiplied by 0.2mL of avian influenza H5N1 virus per chicken 24 hours after the last administration on the third day, and the morbidity and mortality of the chickens in each group are observed within 14 days after the virus is attacked.
As a result, after 3d, 12% of the chickens began to have symptoms, which were manifested by depression, decreased feed intake, and the remaining chickens normally had no infectious symptoms, and the symptomatic chickens only had some symptoms better on day 5, and the statistical mortality rate was 2% after 14 days;
in group B, symptoms appear in 3d, mental depression, food intake is reduced or even abolished, and death, nerve and dyspnea symptoms appear in 7d, which are relieved compared with those in group C, and chickens die in 8-11d after toxicity attack, and the death rate is 53%;
group C developed symptoms beginning at 2 d: depressed spirit, less ingestion and disorder of feather; the typical symptoms of avian influenza appear after 5 d; mouth breathing, dyspnea, swollen head lacrimation, unstable standing, weakness of hind limbs, severe neurological symptoms such as split, head bending and the like in the late stage of lying, the death is concentrated at 6-10 days after the attack, and the death rate is 90%;
group D developed symptoms beginning at 2D: depressed spirit, less ingestion and disorder of feather; the typical symptoms of avian influenza appear after 3 d; mouth breathing, dyspnea, swollen head lacrimation, unstable standing, weakness of hind limbs, severe neurological symptoms such as split, head bending and the like in the late stage of lying, wherein death is concentrated at 3-8 days after toxin attack, and the death rate is 92%;
group E (blank control) was normal.
From the results, on the premise of the same addition amount, the effect of the bat dung for preventing and treating the avian influenza is not obvious when the bat dung is not directly used for preventing and treating the avian influenza without treatment, the bat dung has a certain effect of preventing and treating the avian influenza after being extracted, and the effect of preventing and treating the avian influenza after the bat dung is extracted by the method and then fermented is obviously higher than that of the extract obtained by only extraction.
Experiment 2, experimental chicken: 200 healthy chickens of 1 day old are purchased from a chicken farm and are randomly divided into A, B groups, each group comprises 100 chickens, 1% of the veterinary drug for preventing avian influenza prepared in the example 2 is added into the basic ration in the test period of the group A, the basic ration is fed to the group A in the test period A, B groups, the feeding amount and the feeding conditions are the same, A, B groups of chickens are manually fed to 30 days old, fresh chicken manure produced by 10 chickens is randomly collected in each group, and the nitrogen, phosphorus and ammonia content of the fresh chicken manure is measured.
Thus, the average nitrogen content in the fresh excrement of the group A chickens is 0.021mg/g, the phosphorus content is 7.2mg/g, and the ammonia gas content is 0.043 mg/kg;
the average nitrogen content in the fresh feces of the chickens in group B was 0.044mg/g, the phosphorus content was 11.8mg/g, and the ammonia content was 0.095 mg/kg.
From the results, the addition of 1% of the veterinary drug for preventing avian influenza, which is obtained by the invention, into the basal diet of chickens can effectively reduce the contents of nitrogen, phosphorus and ammonia in the excrements of chickens and reduce the pollution of the excrements of chickens to the environment.
In summary, the following steps: according to the method for preventing the influenza of the livestock-raising chickens, the obtained veterinary drug for preventing the avian influenza has good effects of preventing and protecting the avian influenza of the chickens, has no residue in chicken bodies, and is not easy to generate drug-resistant strains; the raw materials have low cost, safety and effectiveness, and no toxicity; the additive is added into the daily chicken feed according to the weight ratio of 1 percent, and can also effectively reduce the excretion of nitrogen and phosphorus in chicken manure.
Similarly, it should be appreciated that in the foregoing description of exemplary embodiments of the invention, for the purposes of streamlining the disclosure and aiding in the understanding of one or more of the various inventive aspects, however, the disclosed method should not be construed as reflecting the intent: that the invention as claimed requires more features than are expressly recited in each claim. Rather, as the following claims reflect, inventive aspects lie in less than all features of a single foregoing disclosed embodiment. Thus, the claims following the detailed description are hereby expressly incorporated into this detailed description, with each claim standing on its own as a separate embodiment of this invention.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. A drug for preventing influenza in poultry raising in animal husbandry is characterized in that: the medicine is prepared from the following materials in parts by weight: 80-100 parts of bat dung, 10-20 parts of solidago decurrens, 10-20 parts of liquorice, 1-15 parts of mulberry leaves, 1-10 parts of honeysuckle, 3-13 parts of isatis roots, 1-10 parts of platycodon grandiflorum powder, 10-50 parts of alginic acid, 20-60 parts of calcium lactate and the balance of distilled liquor.
2. The preventive agent for influenza in poultry farming according to claim 1, wherein: the medicine is prepared from the following materials in parts by weight: 90 parts of bat dung, 15 parts of goldenrod herb, 15 parts of liquorice, 8 parts of mulberry leaf, 5 parts of honeysuckle, 8 parts of isatis root, 5 parts of platycodon root powder, 30 parts of alginic acid, 40 parts of calcium lactate and 1000 parts of distilled liquor.
3. The preventive agent for influenza in poultry farming according to claim 1, wherein: the distilled liquor is sorghum liquor with the alcohol concentration of 25-60% vol.
4. The preparation method of the drug for preventing influenza in the livestock breeding of chicken according to claims 1 to 3, which comprises the following steps:
s1, weighing the traditional Chinese medicinal materials, cleaning the goldenrod, liquorice, mulberry leaves, honeysuckle, isatis root and platycodon root powder, removing impurities, slicing, drying, crushing by a ball mill crusher, sieving by a sieve of 20-40 meshes, mixing and crushing into coarse powder for later use;
s2, sterilizing the container: disinfecting all container surfaces with a disinfectant, carrying out overall irradiation through ultraviolet rays for 10-300 minutes, and finally cleaning the containers with distilled water;
s3, feeding: grinding the coarse powder into fine powder by using a superfine grinder, adding 25-60% vol sorghum liquor, alginic acid and calcium lactate which are 5 times of the weight of the fine powder of the night vision sand into the fine powder of the night vision sand, heating to 46-48 ℃, putting into a sterilized container, leaching for 32-36 hours under the action of extraction pressure difference formed by the work of a high-pressure rotor pump, taking supernatant, recovering ethanol under negative pressure, and concentrating to obtain paste with the solid content of 26-42%;
s4, sterilizing the paste under 0.15-0.18Mpa for 30min, inoculating a powdery Mucor rouxii starter with the weight of 0.05-0.08% of the paste;
s5, sealing: sealing the container, standing in a cool and dry place, and fermenting at 30 deg.C for 7 days;
and S6, finally, obtaining fermented paste, drying and grinding the fermented paste to obtain the medicine for preventing the avian influenza, and storing the filled and sealed medicine at 15-20 ℃ for storage.
5. The method for preparing an influenza preventive agent for livestock breeding chickens according to claim 4, wherein the method comprises the steps of: the rotating speed of the ball milling crusher is 500-700 r/min, and the bottom end of a discharge port at the bottom of the ball milling crusher is movably connected with a fine-hole filter screen with a mesh size of 20-50 meshes.
6. The method for preparing an influenza preventive agent for livestock breeding chickens according to claim 4, wherein the method comprises the steps of: the relative humidity of the ultraviolet irradiation environment in the S2 is 50-60%, the temperature is 15-20 ℃, and the ultraviolet intensity is not lower than 2 of 70 uW/cm.
7. The method for preparing an influenza preventive agent for livestock breeding chickens according to claim 4, wherein the method comprises the steps of: the vacuum degree generated by the working of the high-pressure rotor pump in the S3 is-0.092 to-0.095 MPa, and the discharge pressure is 0.2 to 0.4 MPa.
8. The method for preparing an influenza preventive agent for livestock breeding chickens according to claim 4, wherein the method comprises the steps of: drying the fermented condensed paste at the vacuum degree of 0.03-0.035 MPa and the temperature of 68 ℃ for 12 hours, and grinding to obtain the medicine for preventing avian influenza.
9. The method for preventing influenza in livestock breeding chickens according to claims 1 to 3, wherein the method comprises mixing fine powder of influenza prevention drugs with feed to feed.
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