CN103623079A - Preparation method and use of spine date seed extract having effects of protecting liver and bladder and improving immunity - Google Patents
Preparation method and use of spine date seed extract having effects of protecting liver and bladder and improving immunity Download PDFInfo
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- CN103623079A CN103623079A CN201210296603.1A CN201210296603A CN103623079A CN 103623079 A CN103623079 A CN 103623079A CN 201210296603 A CN201210296603 A CN 201210296603A CN 103623079 A CN103623079 A CN 103623079A
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- ziziphi spinosae
- semen ziziphi
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Abstract
The invention relates to spine date seed extract having effects of protecting liver and bladder and improving immunity and its preparation method and use. The preparation method of the spine date seed extract comprises the following steps of drying spine date seeds by microwave, carrying out crushing and sieving, carrying out degreasing, carrying out immersion extraction by ethanol, carrying out extraction by ethyl acetate, and carrying out pressure reduction distillation drying. The spine date seed extract can be processed by the conventional methods to form tablets, granules, a freeze-dried powder injection and an oral solution. The spine date seed extract can be used for preparation of a traditional Chinese medicine composition having effects of protecting liver and bladder and improving immunity, and can replace antibiotics for production practices of prevention and treatment on livestock and poultry diseases thereby preventing drug residues, guaranteeing animal source food safety and eliminating the animal product export trade barrier.
Description
Technical field
The invention belongs to field of veterinary, especially a kind of have preparation method and an application thereof that protects the liver the Semen Ziziphi Spinosae extract that protects gallbladder, enhancing human body immunity power effect.
Background technology
Increase along with development and the feed additive of China's aviculture, the diseases such as birds liver and gall also and then increase, and especially the occurrence frequency such as broiler HUANGBAI(sic) dysentery is particularly high, is also usually accompanied by immunity of organisms to decline simultaneously, occur in the stage of brooding, greatly reduce the survival rate of poultry.Damage raiser's economic benefit, destroyed the optimum situation of poultry outlet.This have protect the liver the Semen Ziziphi Spinosae extract that protects gallbladder, enhancing human body immunity power effect have calm, protect the liver the effect of protecting gallbladder enhancing immunity, after oral administration, can promote young bird digestion, improve appetite, by regulating immunity to strengthen resistance against diseases, in addition, all right growth promotion indirectly, anti-stress, avoids the raw drug residue causing with antibiotic, cost is low, has no side effect.
Summary of the invention
The object of the present invention is to provide a kind of have protect the liver the Semen Ziziphi Spinosae extract that protects gallbladder, enhancing human body immunity power effect.
Another object of the present invention is to provide the preparation method of above-mentioned Semen Ziziphi Spinosae extract.
The 3rd object of the present invention is to provide above-mentioned Semen Ziziphi Spinosae extract and has in preparation the application protecting the liver in the pharmaceutical composition that protects gallbladder, enhancing human body immunity power effect.
Semen Ziziphi Spinosae extract of the present invention, it makes by the following method: after getting Semen Ziziphi Spinosae microwave drying, pulverize and sieve, defat, extracts by 75%-95% soak with ethanol, then use ethyl acetate extraction, evaporated under reduced pressure;
Described drying means is with the dry 0.5-3 hour of microwave radiation; The method of described defat is the petroleum ether that by volume weight ratio adds Semen Ziziphi Spinosae 3-9 doubly to measure.Soak degreasing 4-8 time, each 2-8h, volatilizes petroleum ether after defat completely; Described immersion leaching process is according to envelope-bulk to weight ratio, to add 3-9 doubly to measure 75%-95% soak with ethanol to extract 2-8 time, and each 0.5-3 hour extracts and goes out ethanol after complete; Described extraction process is to add 2-5 times of water gaging to dissolve according to envelope-bulk to weight ratio, then adds ethyl acetate continuous extraction 3-5 time that 1.5-6 doubly measures.
Above-mentioned Semen Ziziphi Spinosae extract contains jujuboside A:5.86-20.35mg/g, jujuboside B0.56-1.75mg/g.
Semen Ziziphi Spinosae extract of the present invention can be used for preparing to have protecting the liver the Chinese medicine composition that protects gallbladder, enhancing human body immunity power effect, described compositions is by Semen Ziziphi Spinosae extract and conventional adjuvant or carrier, the tablet being prepared into according to conventional formulation method, granule, lyophilized injectable powder, oral solution.Wherein, oral solution can according to every day 50-100mg/kg body weight mode to poultry medication, continuously oral or spice administration in 4-7 days.
The specific embodiment
The present invention is described in further detail by following examples.It should be noted that: following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.
The preparation of embodiment 1 Semen Ziziphi Spinosae extract
By 150g Semen Ziziphi Spinosae in microwave oven 60 ℃, dry 2h, then pulverized 10 mesh sieves, sieved thing and added 5 times of amount petroleum ether soak degreasing 5h, after defat completely, volatilize petroleum ether, then to Semen Ziziphi Spinosae residue, add 5 times of amount 75% soak with ethanol 2h, extract continuously 5 times, extract obtained, add 3 times of water gagings to stir after removing ethanol, then the ethyl acetate continuous extraction 4 times that adds 5 times of amounts, concentrate drying, makes 8.5g Semen Ziziphi Spinosae extractum, and its extraction ratio is 5.67%.
The preparation of embodiment 2 Semen Ziziphi Spinosae extracts
By 100g Semen Ziziphi Spinosae in microwave oven 60 ℃, dry 1.5h, then pulverized 10 mesh sieves, sieved thing and added 3 times of amount petroleum ether soak degreasing 2h, after defat completely, volatilize petroleum ether, then to Semen Ziziphi Spinosae residue, add 3 times of amount 80% soak with ethanol 0.5h, extract continuously 2 times, extract obtained, add 2 times of water gagings to stir after removing ethanol, then the ethyl acetate continuous extraction 3 times that adds 1.5 times of amounts, concentrate drying, makes 5.8g Semen Ziziphi Spinosae extractum, and its extraction ratio is 5.8%.
The preparation of embodiment 3 Semen Ziziphi Spinosae extracts
By 150g Semen Ziziphi Spinosae in microwave oven 60 ℃, dry 3h, then pulverized 10 mesh sieves, sieved thing and added 9 times of amount petroleum ether soak degreasing 8h, after defat completely, volatilize petroleum ether, then to Semen Ziziphi Spinosae residue, add 9 times of amount 95% soak with ethanol 3h, extract continuously 8 times, extract obtained, add 5 times of water gagings to stir after removing ethanol, then the ethyl acetate continuous extraction 5 times that adds 6 times of amounts, concentrate drying, makes 9.6g Semen Ziziphi Spinosae extractum, and its extraction ratio is 6.4%.
The preparation of embodiment 4 Ziziphus Jujuba Mill Granules agent
The Semen Ziziphi Spinosae extractum 50g that embodiment 1 is made, dextrin 100g, sucrose 50g fully mixes, and granulation is dried, granulate, crossing 16 mesh sieves can make.
The effect test of embodiment 1 Semen Ziziphi Spinosae extract treatment AA broiler liver-gallbladder disease
The AA chickling of selecting 28 age in days left and right to suffer from liver-gallbladder disease is divided into 5 groups, every group 15, be respectively basic, normal, high three the dosage groups of Semen Ziziphi Spinosae extract, polygynax group, negative control group (referring to not administration group), separately establish a Normal group (neither administration also not counteracting toxic substances).Except Normal group, in administration after four days, with 0.5ml/ dosage lumbar injection only, easily cause the escherichia coli syr-a strain bacterium liquid of broiler liver-gallbladder disease, continuous use 3 days again after counteracting toxic substances, two negative control group and Normal group give equivalent normal saline, Continuous Observation 10 days, at viewing duration, observe and respectively organize clinical symptoms, and record dead chicken number, cut open inspection and determine the cause of death, result is as shown in table 1.
The effect test of table 1 Semen Ziziphi Spinosae extract treatment AA broiler liver-gallbladder disease
Grouping | Sample size | Cure rate/% | Death toll/only | Effective percentage/% |
Semen Ziziphi Spinosae high dose group | 15 | 86.7 | 0 | 100% |
Dosage group in Semen Ziziphi Spinosae | 15 | 80 | 1 | 93.3 |
Semen Ziziphi Spinosae low dose group | 15 | 53.3 | 3 | 73.3 |
Polygynax group | 15 | 40 | 5 | 66.7 |
Negative control group | 15 | 0 | 10 | 0 |
Normal group | 15 | - | 0 | - |
In table, data show, the cure rate of Semen Ziziphi Spinosae extract high dose group is the highest, reaches 86.7%, are significantly higher than polygynax combination negative control group.
Embodiment 2 Semen Ziziphi Spinosae extracts strengthen the effect test of laying chickling premunition
Xinyang county, Shandong AA of plant chickling, 35 ages in days, 10000, minute I, two Ji Lan of II, I the full group's outburst of Ji Lan newcastle rate reaches more than 85%, and II the full group of Ji Lan sickness rate reaches more than 90%, and there is death in indivedual chickens.Its jumpbogroup feature is: feed intake reduces, and dyspnea occurs that muscular stomach expands, inappetence.Cut open inspection: glandular stomach thelorrhagia, glandular stomach and muscular stomach intersection have slight hemorrhage band.Therapeutic Method is: Semen Ziziphi Spinosae extract according to every day 50mg/kg body weight mode to chickling medication, spice administration in continuous 5 days.
Table 2 medication within the 3rd day, start to observe jumpbogroup situation:
Number of cases | A Ji Lan | B Ji Lan |
Ill young number/only | 6000 | 4000 |
Healing number/only | 5920 | 3940 |
Death toll/only | 20 | 60 |
Cure rate/% | 98.67 | 98.5 |
By upper table, can be learnt, Semen Ziziphi Spinosae extract can be realized higher premunition by strengthening chickling herd immunity, wherein to the healing of ill chickling up to more than 98.5%, the ill symptoms of not curing chicken also takes a turn for the better gradually.
Claims (3)
1. Semen Ziziphi Spinosae extract has in preparation the application protecting the liver in the Chinese medicine composition that protects gallbladder, enhancing human body immunity power effect, described Semen Ziziphi Spinosae extract makes by the following method: after getting Semen Ziziphi Spinosae microwave drying, pulverize and sieve, defat, by 75%-95% soak with ethanol, extract, use again ethyl acetate extraction, evaporated under reduced pressure;
Described drying means is with the dry 0.5-3 hour of microwave radiation; The method of described defat is the petroleum ether that by volume weight ratio adds Semen Ziziphi Spinosae 3-9 doubly to measure.Soak degreasing 4-8 time, each 2-8h, volatilizes petroleum ether after defat completely; Described immersion leaching process is according to envelope-bulk to weight ratio, to add 3-9 doubly to measure 75%-95% soak with ethanol to extract 2-8 time, and each 0.5-3 hour extracts and goes out ethanol after complete; Described extraction process is to add 2-5 times of water gaging to dissolve according to envelope-bulk to weight ratio, then adds ethyl acetate continuous extraction 3-5 time that 1.5-6 doubly measures.
2. application as claimed in claim 1, is characterized in that, described compositions is by Semen Ziziphi Spinosae extract and conventional adjuvant or carrier, the tablet being prepared into according to conventional formulation method, granule, lyophilized injectable powder, oral solution.
3. application as claimed in claim 2, is characterized in that, according to every day 50-100mg/kg body weight mode to poultry medication, continuously oral or spice administration in 4-7 days.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105010853A (en) * | 2015-07-09 | 2015-11-04 | 徐伟明 | Early starter disease-resisting and fattening feed for bighead carp and silver carp |
CN110521871A (en) * | 2019-09-20 | 2019-12-03 | 深圳市红瑞生物科技有限公司 | It is a kind of alleviate acute and chronic stress to pet injure nutritional preparation and its application |
CN113016844A (en) * | 2021-03-15 | 2021-06-25 | 山西中医药大学 | Semen ziziphi spinosae extract with coffee flavor and liver protection function and preparation method and application thereof |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN1548132A (en) * | 2003-05-15 | 2004-11-24 | 北京群子源生物技术开发有限公司 | Common jujube seed extract and its use |
CN101455736A (en) * | 2008-12-31 | 2009-06-17 | 中国农业大学 | Wild jujube seeds extract and preparation method and use thereof |
CN101926865A (en) * | 2010-09-11 | 2010-12-29 | 天津医科大学 | Spina date seed depression-resolving and nerve-soothing composition and preparation method thereof |
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2012
- 2012-08-21 CN CN201210296603.1A patent/CN103623079A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN1548132A (en) * | 2003-05-15 | 2004-11-24 | 北京群子源生物技术开发有限公司 | Common jujube seed extract and its use |
CN101455736A (en) * | 2008-12-31 | 2009-06-17 | 中国农业大学 | Wild jujube seeds extract and preparation method and use thereof |
CN101926865A (en) * | 2010-09-11 | 2010-12-29 | 天津医科大学 | Spina date seed depression-resolving and nerve-soothing composition and preparation method thereof |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105010853A (en) * | 2015-07-09 | 2015-11-04 | 徐伟明 | Early starter disease-resisting and fattening feed for bighead carp and silver carp |
CN110521871A (en) * | 2019-09-20 | 2019-12-03 | 深圳市红瑞生物科技有限公司 | It is a kind of alleviate acute and chronic stress to pet injure nutritional preparation and its application |
CN113016844A (en) * | 2021-03-15 | 2021-06-25 | 山西中医药大学 | Semen ziziphi spinosae extract with coffee flavor and liver protection function and preparation method and application thereof |
CN113016844B (en) * | 2021-03-15 | 2024-02-27 | 山西中医药大学 | Wild jujube seed extract with coffee flavor and liver protection, and preparation method and application thereof |
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Application publication date: 20140312 |