CN111150681B

CN111150681B - Moisturizing compound containing symbiotic bacteria combined fermentation product, moisturizing essence and preparation method of moisturizing compound

Info

Publication number: CN111150681B
Application number: CN202010121165.XA
Authority: CN
Inventors: 龚德明; 温伟红; 莫霖霭; 容惠
Original assignee: Guangzhou Biotechnology Co ltd
Current assignee: Guangzhou Biotechnology Co.,Ltd.
Priority date: 2020-02-26
Filing date: 2020-02-26
Publication date: 2021-08-17
Anticipated expiration: 2040-02-26
Also published as: CN111150681A

Abstract

The invention provides a moisturizing compound containing symbiotic bacteria combined leavening, moisturizing essence and a preparation method thereof. The moisturizing compound comprises: symbiotic bacteria combined fermentation product, white truffle extract, rhizobium glue, saccharide isomerate, hyaluronic silanol, medium molecular sodium hyaluronate, small molecular sodium hyaluronate, micro molecular sodium hyaluronate, ceramide 3 and beta-glucan. The moisturizing compound meets the moisturizing requirements of all layers of the epidermis of the skin, and achieves the purposes of reducing the transdermal water loss rate and promoting the generation of hyaluronic acid. The moisturizing essence can enhance the internal cell water circulation, strengthen the surface water-locking barrier and the like, and achieve full-effect moisturizing care.

Description

Moisturizing compound containing symbiotic bacteria combined fermentation product, moisturizing essence and preparation method of moisturizing compound

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to moisturizing essence and a preparation method thereof.

Background

At present, people pay more and more attention to skin care, the basic skin care work for beauty lovers is very important, the basic procedure of skin moisturizing is well done, skin aging can be delayed, skin peeling, acne growing and the like are avoided, the skin is kept in a good state, and the efficacy of moisturizing cosmetics meets the requirement. The existing moisturizing cosmetics have the defects of single function and limited water locking and moisturizing functions, and the single moisturizing way and the single moisturizing channel cause the moisturizing effect to be tiny, so that the ideal moisturizing state cannot be achieved frequently; or in order to improve the moisturizing effect, the risk components such as essence, pigment, hormone, lead and mercury are added illegally, so that the skin immunity is easily reduced, and the skin is gradually fragile and frequently problematic.

CN109602620A discloses a powerful moisturizing essence and a preparation method thereof, wherein the powerful moisturizing essence comprises the following raw materials in parts by weight: 5-10 parts of maltooligosyl glycoside, 4-7 parts of hydrogenated starch hydrolysate, 10-15 parts of stearyl alcohol, 2-5 parts of sodium polyacrylate, 5-10 parts of poly (glycerol methacrylate), 1-3 parts of benzophenone, 1-2 parts of hyaluronic acid, 1-4 parts of monoglyceride, 2-5 parts of carboxymethyl cellulose and a proper amount of deionized water; the strong moisturizing essence obtained by the invention has good moisturizing performance, but has poor moisturizing effect on stratum corneum, granular layer and acanthosphere of skin.

CN109674734A discloses a composition with moisturizing, repairing, soothing and anti-aging effects and application thereof, wherein the composition comprises oligopeptide-1, trehalose, a peony root extract, bioglycan-1, ceramide 3, a cynanchum atratum extract, alpha-glucan oligosaccharide, yacon root juice, lactobacillus, silantriol, hyaluronic acid and palmitoyl tripeptide-5. The invention also has the problem of poor pertinence to the moisturizing effect of the components of the composition on the stratum corneum, the granular layer and the acanthopanax papyrifera of the skin by adding the composition of oligopeptide-1, trehalose, a paeonia lactiflora root extract, bioglycan-1, ceramide 3, a cynanchum atratum extract, alpha-glucan oligosaccharide, yacon root juice, lactobacillus, silanetriol, hyaluronic acid and palmitoyl tripeptide-5.

Therefore, the development of a moisturizing product capable of addressing the moisturizing needs of each layer of the epidermis of the skin is the focus of current research in the field.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide a moisturizing essence and a preparation method thereof, wherein the moisturizing essence scientifically customizes a compound moisturizing formula according to the moisturizing requirements of each layer of the epidermis of the skin, and customizes scientific moisturizing schemes for different layers of a microecological layer, a cuticle layer, a granular layer, a spinous layer and a basal layer respectively, so that the full-effect moisturizing care of reducing the percutaneous water loss rate, promoting the generation of hyaluronic acid, enhancing the internal cell water circulation, strengthening the surface water-locking barrier and the like is achieved.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the present invention provides a moisturizing complex comprising a fermentation product of a symbiotic composition, the moisturizing complex comprising: symbiotic bacteria combined fermentation product, white truffle extract, rhizobium glue, saccharide isomerate, hyaluronic silanol, medium molecular sodium hyaluronate, small molecular sodium hyaluronate, micro molecular sodium hyaluronate, ceramide 3 and beta-glucan.

According to the invention, the truffle extracted from the truffle contains rich protein and amino acid, can regulate the secretion of facial oil, enables the skin to recover to a normal pH value, and is deep and clean, wherein the glutamic acid can help the skin to effectively moisturize, and the organic citric acid and the malic acid can improve excessively rough skin and help the skin to recover to healthy metabolism. The symbiotic bacteria combined fermentation product and the truffle extract can maintain a good wet state of skin by regulating and controlling microecological flora.

In the invention, the saccharide isomer (lodestone, CAS number: 100843-69-4) is a good water-retaining agent and emollient. The isomerized D-glucan which is prepared by biochemical technology and has the functions of repairing epidermal layer cells and improving the water replenishing capacity of skin has a composition structure similar to the human cuticle, can be combined with epsilon-amino acid functional groups when being used on the skin, is firmly combined like a magnet, and can maintain the water magnetic field capacity of the skin for a long time. According to the invention, through the synergistic effect of three humectants, namely saccharide isomerate, macromolecular hyaluronic acid, rhizobium glue and the like, an invisible water retention membrane is formed on the horny layer, and continuous water locking, water capturing and water absorbing care is provided for the skin, so that the skin percutaneous water loss rate is reduced.

In the present invention, by adding sodium hyaluronate of medium molecular weight, it is possible to reach the granular layer, replenish water to the granular layer, and promote the synthesis of hyaluronic acid. According to the invention, the small-molecule acetylated sodium hyaluronate is added to carry a large amount of water to reach the position, so that the dry skin is full. According to the invention, micro-molecular sodium hyaluronate is added to reach the deepest part of epidermis, and the self water retention of skin is improved by promoting lipid water circulation in cells.

According to the invention, in order to aim at dry skin, the ceramide 3 and the beta-glucan are particularly added, so that on one hand, the barrier function of a dry stratum corneum is enhanced, on the other hand, the inflammatory reaction caused by dryness is effectively repaired and inhibited, the skin can be relieved, and the desquamation and sensitivity problems caused by dryness are reduced.

Preferably, the moisturizing compound comprises the following components in parts by weight: 1-10 parts of symbiotic bacteria combined leavening, 0.01-5 parts of truffle extract, 0.01-5 parts of rhizobium glue, 0.01-5 parts of saccharide isomer, 0.01-5 parts of hyaluronic silanol, 0.01-5 parts of medium molecular sodium hyaluronate, 0.01-5 parts of small molecular sodium hyaluronate, 0.01-5 parts of micro molecular sodium hyaluronate, 30.1-10 parts of ceramide and 0.1-10 parts of beta-glucan.

In the present invention, the weight parts of the symbiotic bacteria combined fermentation product are 1-10 parts, for example, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts.

In the present invention, the amount of the truffle extract is 0.01 to 5 parts by weight, and may be, for example, 0.01 part, 0.05 part, 0.1 part, 0.5 part, 1 part, 1.5 parts, 2 parts, 3 parts, 4 parts, or 5 parts.

In the present invention, the rhizobium glue is used in an amount of 0.01 to 5 parts by weight, for example, 0.01 part, 0.05 part, 0.1 part, 0.5 part, 1 part, 1.5 parts, 2 parts, 3 parts, 4 parts, 5 parts.

In the present invention, the saccharide isomer may be used in an amount of 0.01 to 5 parts by weight, for example, 0.01 part, 0.05 part, 0.1 part, 0.5 part, 1 part, 1.5 parts, 2 parts, 3 parts, 4 parts or 5 parts.

In the present invention, the hyaluronic acid silanol may be present in an amount of 0.01 to 5 parts by weight, for example, 0.01 part, 0.05 part, 0.1 part, 0.5 part, 1 part, 1.5 parts, 2 parts, 3 parts, 4 parts, and 5 parts.

In the present invention, the weight parts of the medium molecule sodium hyaluronate are 0.01-5 parts, for example, 0.01 part, 0.05 part, 0.1 part, 0.5 part, 1 part, 1.5 parts, 2 parts, 3 parts, 4 parts and 5 parts.

In the present invention, the weight part of the small molecule acetylated sodium hyaluronate is 0.01-5 parts, for example, 0.01 part, 0.05 part, 0.1 part, 0.5 part, 1 part, 1.5 parts, 2 parts, 3 parts, 4 parts and 5 parts.

In the present invention, the micro-molecular sodium hyaluronate may be used in an amount of 0.01 to 5 parts by weight, for example, 0.01, 0.05, 0.1, 0.5, 1, 1.5, 2, 3, 4, or 5 parts by weight.

In the present invention, the ceramide 3 may be, for example, 0.1 to 10 parts by weight, such as 0.1 part, 0.5 part, 1 part, 1.5 parts, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, and 10 parts.

In the present invention, the weight part of the β -glucan is 0.1 to 10 parts, and may be, for example, 0.1 part, 0.5 part, 1 part, 1.5 parts, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, or 10 parts.

Preferably, the symbiotic bacteria of the symbiotic bacteria combined fermentation product comprise a combination of schizophyllum commune, thermus thermophilus, saccharomycetes and lactobacillus, and the fermentation raw materials of the symbiotic bacteria combined fermentation product comprise rice, Sichuan millet seeds, ginseng, kelp and raspberry.

Preferably, the mass ratio of schizophyllum commune, thermus thermophilus, yeast and lactobacillus in the symbiotic bacteria is (8-18): (4-8): (2-6): (1-3).

In the invention, the mass ratio of schizophyllum commune, thermus thermophilus, microzyme and lactobacillus in the symbiotic bacteria is (8-18): (4-8): (2-6): (1-3), and the further optimization is 12:3:2: 1.

Wherein, the 8-18 can be 9, 10, 11, 12, 13, 14, 15, 16 or 17, etc.

The 4-8 can be 4.2, 4.5, 4.8, 5, 5.2, 5.5, 5.8, 6, 6.2, 6.5, 6.8, 7, 7.2, 7.5, 7.7, or 7.9, etc.

The 2-6 can be 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5, 4.8, 5, 5.2, 5.5, 5.7, or 5.9, etc.

The 1-3 can be 1.2, 1.4, 1.6, 1.8, 2, 2.2, 2.4, 2.6, 2.8, 2.9, or the like.

Preferably, the mass ratio of the rice, the Sichuan millet seeds, the ginseng, the kelp and the raspberry in the fermentation raw materials is (40-60): (40-60):1: (20-30): 20-30).

In the above mass ratio, two of 40 to 60 may be 42, 44, 45, 46, 48, 50, 52, 54, 55, 56, 58, etc. independently; the two 20-30 each independently can be 22, 24, 25, 26, 28, 30, etc.

Preferably, the mass sum of the symbiotic bacteria is 20-30% of the mass sum of the fermentation raw materials, and may be, for example, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, or the like.

In the invention, the preparation method of the symbiotic bacteria combined fermentation product comprises the following steps:

(1) pulverizing rice, Sichuan millet seed, ginseng, kelp and raspberry, mixing with water to obtain a to-be-fermented solution, and sterilizing;

(2) inoculating yeast and lactobacillus to the sterilized liquid to be fermented obtained in the step (1) for primary fermentation, then adding schizophyllum commune for secondary fermentation, and finally adding thermophilic thermus bacteria for tertiary fermentation to obtain the symbiotic bacteria combined fermentation product.

In the preparation process of the symbiotic bacteria combined fermentation product, different living environments of different strains are considered, so that the fermentation process is performed step by step, firstly, yeast and lactobacillus are subjected to enriched fermentation, then schizophyllum commune is added for fermentation, the yeast and the lactobacillus have an induced activation effect on the schizophyllum commune, the generation of schizophyllum commune can be increased, the generated schizophyllum commune further has an accelerating effect on the yeast and the lactobacillus to accelerate the fermentation of the yeast and the lactobacillus to raw materials, and finally, thermophilic thermus bacteria are added, and the yeast also has an induced activation effect on the thermophilic thermus bacteria, so that the high-activity symbiotic bacteria combined fermentation product is obtained.

In the present invention, the method of culturing yeast, lactobacillus, thermus thermophilus, and schizophyllum to satisfy the inoculation requirements in terms of the number and activity is not particularly limited, and any conventional method in the prior art may be used.

Preferably, the hyaluronan silanol consists of a macromolecular hyaluronic acid and a silanetriol.

The hyaluronic acid silanol has an irritation effect on keratinocytes, can quickly increase the thickness of epidermis, and can better preserve moisture and protect skin; under the condition that skin is damaged by hormone, flagella at the outer edge of cells can be damaged, and the silanol hyaluronate can repair the flagella at the outer edge of cells, so that beneficial substances can be absorbed, and harmful substances can be eliminated, so that the existing cells are healthier.

Preferably, the mass ratio of the macromolecular hyaluronic acid to the silanetriol is (2-4): 6-8, and can be, for example, 2:8, 3:7, 4:6, 2:7, 3:6, 3:8, 4:7, 4: 8.

Preferably, the molecular weight of the macromolecular hyaluronic acid is between 150 and 180 kilodaltons, and may be, for example, 150 kilodaltons, 155 kilodaltons, 160 kilodaltons, 165 kilodaltons, 170 kilodaltons, 175 kilodaltons, 180 kilodaltons.

Preferably, the molecular weight of the medium molecule sodium hyaluronate is between 80 and 100 kilodaltons, such as 80 kilodaltons, 82 kilodaltons, 84 kilodaltons, 86 kilodaltons, 88 kilodaltons, 90 kilodaltons, 92 kilodaltons, 94 kilodaltons, 96 kilodaltons, 98 kilodaltons, 100 kilodaltons.

Preferably, the small molecule sodium hyaluronate has a molecular weight of 20-40 kilodaltons, e.g. 20 kilodaltons, 22 kilodaltons, 24 kilodaltons, 26 kilodaltons, 28 kilodaltons, 30 kilodaltons, 32 kilodaltons, 34 kilodaltons, 36 kilodaltons, 38 kilodaltons, 40 kilodaltons.

Preferably, the micro-molecular sodium hyaluronate has a molecular weight below 1 kilodalton, such as 1000 daltons, 2000 daltons, 3000 daltons, 4000 daltons, 5000 daltons, 6000 daltons, 7000 daltons, 8000 daltons, 9000 daltons, 10000 daltons.

In a second aspect, the present invention provides a moisturizing essence comprising the moisturizing compound comprising the symbiotic bacteria combined ferment of the first aspect.

Preferably, the moisturizing essence comprises the following components in percentage by mass: 5-20% of the moisturizing compound containing the symbiotic bacteria combination fermentation product, 1-30% of a humectant, 5-20% of an emollient, 1-10% of an emulsifier, 0.5-2% of a skin conditioner and the balance of rose water.

In the moisturizing essence, the content of the moisturizing compound containing the symbiotic bacteria combined fermentation product is 5-20%, for example, 5%, 6%, 8%, 10%, 12%, 14%, 16%, 18% and 20%.

In the moisturizing essence, the content of the moisturizing agent is 1-30%, and can be, for example, 1%, 2%, 4%, 6%, 8%, 10%, 12%, 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30%.

In the moisturizing essence provided by the invention, the content of the emollient is 5-20%, for example, 5%, 6%, 8%, 10%, 12%, 14%, 16%, 18%, 20%.

In the moisturizing essence, the content of the emulsifier is 1-10%, and can be 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, for example.

In the moisturizing essence, the content of the skin conditioner is 0.5-2%, for example, 0.5%, 0.6%, 0.8%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%.

Preferably, the humectant comprises any one of, or a combination of at least two of, 1, 2-hexanediol, butanediol, glycerol, panthenol, xylitol, anhydroxylitol or xylitol-based glucoside.

Preferably, the emollient comprises any one or a combination of at least two of squalane, canola oil, caprylic/capric triglyceride, dimethicone, or stearic acid.

Preferably, the emulsifier comprises any one or a combination of at least two of cetearyl alcohol, cetearyl glucoside, polyglyceryl-3 methyl glucose distearate, glyceryl stearate, PEG-100 stearate, sodium acrylate copolymer, or lecithin.

Preferably, the skin conditioner comprises any one or a combination of at least two of dipotassium glycyrrhizinate, hydrogenated lecithin, a buddleja officinalis extract, a sophora flavescens root extract, a glycyrrhiza inflate root extract, a scutellaria baicalensis root extract or a narcissus bulb extract, and preferably the combination of the dipotassium glycyrrhizinate, the hydrogenated lecithin, the buddleja officinalis extract, the sophora flavescens root extract, the glycyrrhiza inflate root extract, the scutellaria baicalensis root extract and the narcissus bulb extract.

The flos buddlejae extract, the radix sophorae flavescentis extract, the radix glycyrrhizae inflatae extract, the radix scutellariae extract and the narcissus bulb extract have a synergistic effect, and can promote skin degradation and transdermal absorption of hyaluronic acid and promote skin absorption of nutrition, so that the moisture retention and water locking capacity of the skin is further improved, and the skin firmness and elasticity are restored.

Preferably, the moisturizing essence further comprises 0.02-0.1% of a chelating agent, such as 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%.

Preferably, the chelating agent is disodium EDTA.

Preferably, the moisturizing essence further comprises 0.01-0.1% of a thickening agent.

Preferably, the thickener is hydrolyzed sclerotium rolfsii gum.

Preferably, the moisturizing essence further comprises 0.5-2% of an antioxidant, which may be, for example, 0.5%, 0.6%, 0.8%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%.

Preferably, the antioxidant is p-hydroxyacetophenone and/or tocopherol acetate.

In a third aspect, the present invention provides a method for preparing the moisturizing essence according to the second aspect, the method comprising the steps of:

(1) mixing the humectant and the rose water to obtain a water-phase mixed solution; mixing an emollient and an emulsifier in an oil phase to obtain an oil phase mixed solution;

(2) mixing the water phase mixed liquor and the oil phase mixed liquor obtained in the step (1) and homogenizing to obtain homogenized liquor;

(3) and (3) adding the moisturizing compound containing the symbiotic bacteria combined fermentation product and the skin conditioner into the homogeneous liquid obtained in the step (2), and mixing and stirring to obtain the moisturizing essence.

Preferably, the preparation method comprises the following steps:

(1) mixing the humectant, the chelating agent, the thickening agent and the rose water in a water phase to obtain a water phase mixed solution; mixing an antioxidant, an emollient and an emulsifier in an oil phase to obtain an oil phase mixed solution;

Preferably, the temperature of the aqueous phase mixing in step (1) is 80-85 ℃, for example 80 ℃, 81 ℃, 82 ℃, 83 ℃, 84 ℃, 85 ℃.

Preferably, the time for mixing the water phase in step (1) is 5-10min, such as 5min, 6min, 7min, 8min, 9min, 10 min.

Preferably, the temperature at which the oil phase is mixed in step (1) is 78 to 80 ℃, and may be, for example, 78 ℃, 78.5 ℃, 79 ℃, 79.5 ℃, 80 ℃.

Preferably, the time for mixing the oil phase in step (1) is 5-10min, such as 5min, 6min, 7min, 8min, 9min, 10 min.

Preferably, the temperature for mixing and homogenizing in the step (2) is 80-82 deg.C, such as 80 deg.C, 80.5 deg.C, 81 deg.C, 81.5 deg.C, 82 deg.C.

Preferably, the mixing and homogenizing time in step (2) is 5-10min, such as 5min, 6min, 7min, 8min, 9min, 10 min.

Preferably, the mixing and stirring temperature in step (3) is 40-50 deg.C, such as 40 deg.C, 42 deg.C, 44 deg.C, 46 deg.C, 48 deg.C, 50 deg.C.

Preferably, the mixing and stirring time in step (3) is 5-10min, such as 5min, 6min, 7min, 8min, 9min, 10 min.

Preferably, the preparation method comprises the following steps:

(1) mixing humectant, chelating agent, thickener and flos Rosae Rugosae water at 80-85 deg.C under stirring for 5-10min to obtain water phase mixed solution; stirring and mixing antioxidant, emollient and emulsifier at 78-80 deg.C for 5-10min to obtain oil phase mixed solution;

(2) mixing the water phase mixed liquor and the oil phase mixed liquor obtained in the step (1) at 80-82 ℃ and homogenizing for 5-10min to obtain homogenized liquor;

(3) adding the moisturizing compound containing the symbiotic bacteria combined fermentation product and the skin conditioner into the homogenized liquid obtained in the step (2), and mixing and homogenizing at 40-50 ℃ for 5-10min to obtain the moisturizing essence.

Compared with the prior art, the invention has the following beneficial effects:

(1) the moisturizing compound containing the symbiotic bacteria combined fermentation product scientifically customizes a compound moisturizing formula according to the moisturizing requirements of each layer of the skin epidermis, and customizes scientific moisturizing schemes for different layers of a microecological layer, a cuticle layer, a granular layer, a spinous layer and a basal layer respectively, so that the full-effect moisturizing care of reducing the percutaneous water loss rate, promoting the generation of hyaluronic acid, enhancing the internal cell water circulation, strengthening the surface water-locking barrier and the like is achieved.

(2) The moisturizing compound containing the symbiotic bacteria combination fermentation product provided by the invention utilizes the mutual cooperation of the symbiotic bacteria combination fermentation product and the chrysosporium extract, realizes synergistic interaction, and achieves the effects of moisturizing and water locking by regulating and controlling microecological flora and particularly aiming at a skin microbial layer.

(3) The moisturizing compound containing the symbiotic bacteria combination fermentation product disclosed by the invention is synergistic through the mutual cooperation of saccharide isomerate, macromolecular hyaluronic acid and rhizobium glue, an invisible water retention film is formed on the horny layer, continuous water locking, water capturing and water absorbing care is provided for the skin, and the percutaneous water loss rate of the skin is reduced.

(4) The moisturizing compound containing the symbiotic bacteria combined leavening reaches the granular layer through the hyaluronic acid molecules, so that moisture is supplemented to the granular layer, and the synthesis of the hyaluronic acid of the skin is promoted.

(5) The moisturizing compound containing the symbiotic bacteria combined fermentation product carries a large amount of water to a spinous layer through micromolecular acetylated hyaluronic acid, so that the spinous layer is full of shrunken cells, and the dry skin is restored to be full.

(6) The moisturizing compound containing the symbiotic bacteria combination fermentation product reaches the deepest part (basal layer) of the epidermis through micromolecular hyaluronic acid, and improves the self water-retaining capacity of the skin by promoting lipid water circulation in cells.

Detailed Description

The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.

In the following examples, the symbiotic combination fermentate may be prepared by the following method:

(1) pulverizing 33.11g rice, 33.11g Sichuan millet seed, 0.66g Ginseng radix, 16.56g Macrocystis and 16.56g Rubi fructus, mixing with 180g water, filtering with 80 mesh filter cloth to obtain fermentation solution, and heating at 100 deg.C for 20min for sterilization;

(2) adjusting pH of the sterilized solution to be fermented to 4.5 with arginine, placing in a seed tank, inoculating yeast 4g and lactobacillus 2g, and culturing at 5m³Aeration amount of/h/50L, stirring and rotating at 150rpm, and culturing at 30 ℃ for 22 h;

(3) then adding arginine to adjust pH to 6.5, adding 12g schizophyllum commune at 5m³Aeration amount of/h/50L, stirring and rotating at 150rpm, and culturing at 30 ℃ for 22 h;

(4) finally, arginine was used to adjust the pH to 8.0, 6g of Thermus thermophilus was added at 5m³Stirring and rotating at 150rpm for ventilation amount of 50L/h, culturing at 35 ℃ for 22h, and then raising the temperature to 58 ℃ for culturing for 18h to obtain a primary product;

(5) filtering the primary product, taking clear liquid, adding active carbon into the clear liquid, stirring for 10min, performing adsorption and purification treatment, taking clear liquid again by using a filtering mode, and sterilizing to obtain the symbiotic bacteria combined fermentation product.

Wherein the rice is northeast rice, fructus oryzae is from Guizhou, Ginseng radix is Changbai mountain Ginseng radix, Macrocystis is from Shandong Changdao county, and Rubi fructus is from Anhui. The yeast is selected from cerevisiae Fermentum, and the lactobacillus is selected from Lactobacillus thermophilus.

Example 1

The embodiment provides a moisturizing compound containing symbiotic bacteria combined leavening, which comprises the following components in parts by weight: 5 parts of symbiotic bacteria combined fermentation product, 4 parts of truffle extract, 3 parts of rhizobium glue, 1 part of saccharide isomer, 2 parts of hyaluronic silanol (the mass ratio of macromolecular hyaluronic acid to silanetriol is 3:7, and the molecular weight is 150 kilodalton), 2 parts of medium molecular sodium hyaluronate (the molecular weight is 90 kilodalton), 2 parts of small molecular sodium hyaluronate (the molecular weight is 30 kilodalton), 2 parts of micro molecular sodium hyaluronate (the molecular weight is below 3000 kilodalton), 34 parts of ceramide and 3 parts of beta-glucan.

Example 2

The embodiment provides a moisturizing compound containing symbiotic bacteria combined leavening, which comprises the following components in parts by weight: 6 parts of symbiotic bacteria combined fermentation product, 3 parts of truffle extract, 4 parts of rhizobium glue, 2 parts of saccharide isomer, 1 part of hyaluronic silanol (mass ratio of macromolecular hyaluronic acid to silanetriol is 2:8 and molecular weight is 150 kilodaltons), 3 parts of medium molecular sodium hyaluronate (molecular weight is 100 kilodaltons), 3 parts of small molecular sodium hyaluronate (molecular weight is 20 kilodaltons), 4 parts of micro molecular sodium hyaluronate (molecular weight is 1 kilodaltons), 32 parts of ceramide and 4 parts of beta-glucan.

Example 3

The embodiment provides a moisturizing compound containing symbiotic bacteria combined leavening, which comprises the following components in parts by weight: 6 parts of symbiotic bacteria combined leavening, 3 parts of truffle extract, 4 parts of rhizobium glue, 2 parts of saccharide isomer, 1 part of hyaluronic silanol (the mass ratio of macromolecular hyaluronic acid to silanetriol is 4:6, and the molecular weight is 180 ten thousand daltons), 3 parts of medium molecular sodium hyaluronate (the molecular weight is 80 ten thousand daltons), 3 parts of small molecular sodium hyaluronate (the molecular weight is 40 ten thousand daltons), 4 parts of micro molecular sodium hyaluronate (the molecular weight is 5000 daltons), 32 parts of ceramide and 4 parts of beta-glucan.

Example 4

This example provides a moisturizing composition comprising a symbiotic combination ferment, differing from example 1 only in that: the mass ratio of the macromolecular hyaluronic acid to the silanetriol in the hyaluronic acid silanol is 1:9, and the contents of other components are unchanged.

Example 5

This example provides a moisturizing composition comprising a symbiotic combination ferment, differing from example 1 only in that: the mass ratio of the macromolecular hyaluronic acid to the silanetriol in the hyaluronic acid silanol is 9:1, and the contents of other components are unchanged.

Example 6

This example provides a moisturizing composition comprising a symbiotic combination ferment, differing from example 1 only in that: the content of the symbiotic bacteria combined fermentation product is reduced to 1 part, and the content of other components is unchanged.

Example 7

This example provides a moisturizing composition comprising a symbiotic combination ferment, differing from example 1 only in that: the content of the symbiotic bacteria combined fermentation product is increased to 10 parts, and the content of other components is unchanged.

Comparative examples 1 to 10

The moisture-keeping compound containing symbiotic bacteria combined leavening is shown in the following table 1 according to the formula of each proportion in parts by weight (as shown in the table, 5 represents that the content of the corresponding component is 5 parts by weight):

TABLE 1

Application example 1

The application embodiment provides a moisturizing essence which comprises the following components in percentage by mass:

the preparation method of the moisturizing essence provided by the application embodiment comprises the following steps:

(1) stirring and mixing the humectant, the chelating agent, the thickening agent and the rose water at 82 ℃ for 8min to obtain a water-phase mixed solution; stirring and mixing the antioxidant, the emollient and the emulsifier for 8min at 79 ℃ to obtain an oil phase mixed solution;

(2) mixing the water phase mixed liquor and the oil phase mixed liquor obtained in the step (1) at 81 ℃ and homogenizing for 8min to obtain homogenized liquor;

(3) and (3) adding the moisturizing compound containing the symbiotic bacteria combined fermentation product and the skin conditioner into the homogenized liquid obtained in the step (2), and mixing and homogenizing at 45 ℃ for 8min to obtain the moisturizing essence.

Application example 2

The application example provides a moisturizing essence, which is different from the application example 1 in that the moisturizing compound containing the symbiotic bacteria combined fermentation product prepared in the example 1 is replaced by the symbiotic bacteria combined fermentation product prepared in the example 2, and the content of other components and the preparation method are the same as those in the application example 1.

Application example 3

The application example provides a moisturizing essence, which is different from the application example 1 in that the moisturizing compound containing the symbiotic bacteria combined fermentation product prepared in the example 1 is replaced by the symbiotic bacteria combined fermentation product prepared in the example 3, and the content of other components and the preparation method are the same as those in the application example 1.

Application example 4

The application example provides a moisturizing essence, which is different from the application example 1 in that the moisturizing compound containing the symbiotic bacteria combined fermentation product prepared in the example 1 is replaced by the symbiotic bacteria combined fermentation product prepared in the example 4, and the content of other components and the preparation method are the same as those in the application example 1.

Application example 5

The application example provides a moisturizing essence, which is different from the application example 1 in that the moisturizing compound containing the symbiotic bacteria combined fermentation product prepared in the example 1 is replaced by the symbiotic bacteria combined fermentation product prepared in the example 5, and the content of other components and the preparation method are the same as those in the application example 1.

Application example 6

The application example provides a moisturizing essence, which is different from the application example 1 in that the moisturizing compound containing the symbiotic bacteria combined fermentation product prepared in the example 1 is replaced by the symbiotic bacteria combined fermentation product prepared in the example 6, and the content of other components and the preparation method are the same as those in the application example 1.

Application example 7

The application example provides a moisturizing essence, which is different from the application example 1 in that the moisturizing compound containing the symbiotic bacteria combined fermentation product prepared in the example 1 is replaced by the symbiotic bacteria combined fermentation product prepared in the example 7, and the content of other components and the preparation method are the same as those in the application example 1.

Application example 8

The application example provides a moisturizing essence, and the skin conditioner is different from the application example 1 in that the skin conditioner does not contain a buddleja officinalis extract, the content of a sophora flavescens root extract is increased to 0.1%, the content of a glycyrrhiza inflate root extract is increased to 0.06%, the content of a scutellaria baicalensis root extract is increased to 0.14%, the content of a narcissus bulb extract is increased to 0.18%, and the content of other components and the preparation method are the same as the application example 1.

Application example 9

The application example provides a moisturizing essence, and is different from the application example 1 in that the skin conditioner does not contain a sophora flavescens root extract, the content of a buddleja officinalis extract is increased to 0.1%, the content of a glycyrrhiza inflate root extract is increased to 0.06%, the content of a scutellaria baicalensis root extract is increased to 0.14%, the content of a narcissus bulb extract is increased to 0.18%, and the content of other components and the preparation method are the same as the application example 1.

Application example 10

The application example provides a moisturizing essence, and is different from the application example 1 in that the skin conditioner does not contain the glycyrrhiza inflate linn root extract, the content of the buddleja officinalis extract is increased to 0.09%, the content of the sophora flavescens root extract is increased to 0.09%, the content of the scutellaria baicalensis root extract is increased to 0.13%, the content of the narcissus bulb extract is increased to 0.17%, and the content of other components and the preparation method are the same as the application example 1.

Application example 11

The application example provides a moisturizing essence, and is different from the application example 1 in that the skin conditioner does not contain a scutellaria root extract, the content of a buddleja officinalis extract is increased to 0.11%, the content of a kuh-seng root extract is increased to 0.11%, the content of a glycyrrhiza inflate root extract is increased to 0.07%, the content of a narcissus bulb extract is increased to 0.19%, and the content of other components and the preparation method are the same as the application example 1.

Application example 12

The application example provides a moisturizing essence, and is different from the application example 1 in that the skin conditioner does not contain a narcissus bulb extract, the content of a buddleia officinalis extract is increased to 0.12%, the content of a kuh-seng root extract is increased to 0.12%, the content of a liquorice root extract is increased to 0.08%, the content of a scutellaria baicalensis root extract is increased to 0.16%, and the content of other components and the preparation method are the same as the application example 1.

Application of comparative examples 1 to 10

Compared with the application example 1, the moisturizing compound containing the symbiotic bacteria combined fermentation product prepared in the example 1 is replaced by the moisturizing compound containing the symbiotic bacteria combined fermentation product prepared in the comparative examples 1-10, and the rest components and the content are the same as the application example 1.

Comparative application example 11

Compared with the application example 1, the moisturizing compound containing the symbiotic bacteria combined fermentation product prepared in the example 1 is not contained, and the rest components and the content are the same as the application example 1.

Test example 1

Safety irritation test

(1) Haemolysis test of erythrocytes

Preparation of erythrocyte suspension: selecting healthy rabbits, taking 9mL of blood from heart, adding 1mL of 2% potassium oxalate solution, centrifuging, discarding supernatant, diluting the precipitate to 20mL with 20mmol/L PBS solution, and storing at 4 ℃ for later use.

The moisturizing essences prepared in application examples 1 to 12 and the moisturizing essences prepared in application comparative examples 1 to 11 were selected, diluted to different concentrations with PBS solutions, and 5 concentration gradients were set for each sample. Take 10mL to standMeasuring the dilution of the sample, adding 200 μ L of the above erythrocyte suspension (controlling the final concentration of the sample to be 5, 10, 20, 50, 100mg/mL respectively), using distilled water as whole blood control, PBS solution as negative control, mixing gently, incubating at 37 deg.C for 30min, centrifuging at 2000r/min for 10min, collecting the supernatant, and measuring its absorbance at 560nm (A) with spectrophotometer₅₆₀) Calculating the hemolysis rate according to the following formula;

a standard curve of hemolysis rate vs. sample concentration was plotted, and the sample concentration at which hemolysis occurred in 50% erythrocytes (HD) was calculated₅₀)。

(2) Protein denaturation experiments:

diluting the moisturizing essence prepared in application examples 1-12 and the moisturizing essence prepared in application comparative examples 1-11 to 10g/L with PBS solution, taking 10mL of the dilution of the sample to be tested, adding 200 μ L of the erythrocyte suspension, using distilled water as a blank control, using 1mg/mL of Sodium Dodecyl Sulfate (SDS) solution as a positive control, mixing gently, incubating at 37 ℃ for 30min, centrifuging at 2000r/min for 10min, taking the supernatant, and testing the absorbance A at 540nm and 575nm respectively with a spectrophotometer₅₄₀And A₅₇₅Calculating a protein Denaturation Index (DI) according to the following formula;

wherein R is₁Blank control group a₅₇₅Blank control group A₅₄₀，R₂Experimental group a₅₇₅Experimental group A₅₄₀，R₃Positive control group A₅₇₅Positive control group A₅₄₀。

Evaluating the irritation of the sample to be tested according to the L/D value, wherein the L/D value is HD₅₀DI, erythrocyte hemolysis assay irritation grading criteria are shown in Table 2 below:

TABLE 2

L/D	Grading
		﹥100	Has no irritation
10﹤L/D≤100	Micro-stimulation property
		1﹤L/D≤10	Mild irritation
0.1﹤L/D≤1	Moderate irritation
		L/D≤0.1	Severe irritation

The results of the above-described hemolysis test and protein denaturation test are shown in Table 3 below:

TABLE 3

Test sample	HD₅₀(mg/L)	DI(％)	L/D	Evaluation of
					Application example 1	24729	1.00	﹥100	Has no irritation
Application example 2	25465	1.07	﹥100	Has no irritation
					Application example 3	23124	1.06	﹥100	Has no irritation
Application example 4	21567	1.07	﹥100	Has no irritation
					Application example 5	20725	1.05	﹥100	Has no irritation
Application example 6	19893	1.10	﹥100	Has no irritation
					Application example 7	19863	1.12	﹥100	Has no irritation
Application example 8	19741	1.11	﹥100	Has no irritation
					Application example 9	19831	1.12	﹥100	Has no irritation
Application example 10	19620	1.15	﹥100	Has no irritation
					Application example 11	19831	1.17	﹥100	Has no irritation
Application examples12	20620	1.13	﹥100	Has no irritation
					Application comparative example 1	9735	1.89	﹥100	Has no irritation
Comparative application example 2	12567	1.45	﹥100	Has no irritation
					Comparative application example 3	12149	1.70	﹥100	Has no irritation
Application comparative example 4	12568	1.78	﹥100	Has no irritation
					Comparative application example 5	14671	1.49	﹥100	Has no irritation
Comparative application example 6	11456	1.70	﹥100	Has no irritation
					Application comparative example 7	12731	1.73	﹥100	Has no irritation
Comparative application example 8	14360	1.27	﹥100	Has no irritation
					Comparative application 9	10374	1.92	﹥100	Has no irritation
Comparative application example 10	10378	1.27	﹥100	Has no irritation
					Comparative application example 11	9383	1.56	﹥100	Has no irritation

According to the test results, the irritation of the moisturizing essence prepared in the embodiments 1 to 12 is classified into no irritation, and the moisturizing essence has the characteristics of being mild and no irritation; the sample concentration HD of the moisturizing essence prepared by the invention when 50% of erythrocytes are hemolyzed₅₀HD above 19000mg/L which is far greater than moisturizing essence prepared in comparative examples 1-11₅₀Meanwhile, the protein denaturation index DI is below 1.20 percent and is also obviously smaller than that of the moisturizing essence prepared in comparative examples 1-11, so that the moisturizing essence disclosed by the invention can obviously reduce toxic and side effects and irritation and is safer and more reliable.

Test example 2

In vitro weighing method moisturizing performance test research

The moisturizing essence prepared in application examples 1-12 and the moisturizing essence prepared in application comparative examples 1-11 were weighed to be 0.2g, respectively and uniformly coated on a 5cm by 5cm glass plate to which the microporous vent tape was attached, the glass plate was placed in a constant temperature and humidity dryer, the mass of the glass plate after being placed for 2 hours and 4 hours was measured, and the moisturizing rate was calculated. The moisture retention rate calculation formula is as follows: moisture retention rate inhibition rate/% (M)₂-M₀)/(M₁-M₀)×100％。

Wherein M is₀Is the mass/g, M, of the glass sheet₁For the mass/g, M of the glass plate after sample application₂The glass sheet mass/g after standing in the desiccator for several hours.

The experimental results of measuring the moisturizing rate using the moisturizing essence prepared in examples 1 to 12 and the in vitro weighing method using the moisturizing essence prepared in comparative examples 1 to 11 are shown in the following table 4:

TABLE 4

The experiments show that the moisturizing essence prepared in the application examples 1-12 has a moisturizing rate of more than 75% after 4 hours, and the moisturizing essence has a good moisturizing effect. The moisturizing essence prepared in the comparative examples 1-11 is unsatisfactory in moisturizing rate and moisturizing stability, and shows that the moisturizing essence disclosed by the invention has a synergistic effect due to the mutual cooperation of active components, has an excellent moisturizing effect, can fundamentally relieve the problems of skin dryness and water loss, and further improves the skin firmness and elasticity.

Test example 3

Evaluation of moisturizing Effect

Skin moisture content and skin moisture loss (TEWL) test: the moisturizing essences prepared in application examples 1-12 and the moisturizing essences prepared in comparative examples 1-11 were selected for skin moisture content and skin moisture loss (TEWL) tests, which are recognized in the world

The method, capacitance method, the skin water loss TEWL is a very important parameter for evaluating the function of the skin water protective layer, and has been widely accepted internationally. The more intact the skin protection layer, the higher the moisture content and the lower the value of the skin moisture loss TEWL. The test instruments used for the test were a skin moisture Corneometer test probe and a skin moisture loss (TEWL) TewameterTM300 test probe (Courage + Khazaka, Germany).

The test method specifically comprises the following steps:

100 dry skin people with the age of 20-45 years are selected and randomly divided into 24 groups, and 5 people in each group. Any product (cosmetics, external medicines and oral health products) cannot be used 15 days before the tested part. Before the test, the subjects were asked to wash their face and to sit still in a climate controlled room (22 ± 1 ℃, 50% relative humidity) for more than 20min 2h after washing and to remain relaxed.

In the test, a half-face test is carried out according to a random table, the left and right nasolabial sulcus parts of a volunteer are selected to carry out skin moisture content (%) and moisture loss (TEWL) (%), and the average value of 3 values is taken as the left and right nasolabial sulcus moisture content data (d0) and the nasolabial sulcus skin moisture loss data (d 0). After the test was completed, volunteers were used as required for 28 consecutive days and were asked to return on day 14 (d14) and day 28 (d28) of the test. The test results are shown in table 5:

TABLE 5

The experiments show that the moisturizing essence prepared in the application examples 1-12 has a good moisturizing effect on skin compared with the comparative examples 1-11, can obviously increase the moisture content of the stratum corneum of the skin and reduce the moisture loss of the skin to the outside, and is specifically shown in that the moisture content of the skin is above 16% in the 14 th day, the moisture content of the skin is above 22% in the 28 th day, the moisture loss of the skin is below-15% in the 14 th day and the moisture loss of the skin is below-24% in the 28 th day. The moisturizing essence is prepared by scientifically customizing a compound moisturizing formula according to the moisturizing requirements of each layer of the epidermis of the skin, and respectively customizing scientific moisturizing schemes for different layers of a microecological layer, a cuticle layer, a granular layer, a spinous layer and a basal layer, so that the full-effect moisturizing care of reducing the percutaneous water loss rate, promoting the generation of hyaluronic acid, enhancing the internal cell water circulation, strengthening the surface water-locking barrier and the like is achieved.

Test example 4

Skin elasticity test

The evaluation method comprises the following steps: the moisturizing essence prepared in application examples 1-12 and the moisturizing essence prepared in application examples 1-11 were selected by using a skin elasticity tester MPA580 of CK company, germany, and the skin was sucked into a specific test probe by measuring a negative pressure generated on the surface of the skin before and after the use of the product, and the depth of the skin sucked into the test probe was measured by a non-contact optical test system. The test probe comprised a transmitter and receiver of light, the ratio of which was proportional to the depth of the skin being inhaled, and then analyzed by the MPA software to determine the elastic properties of the skin. The ratio of the amount of springback of the skin without negative pressure to the maximum amount of stretch with negative pressure is closer to 1, indicating better skin elasticity.

The test method comprises the following steps: in the test, a half-face test is carried out according to a random table, consistent volunteers and using methods also last for 28 days, return visits are carried out during the period with D0, D14 and D28, and VISIA image shooting and elasticity test are carried out after the people sit still for 30min in a constant-temperature constant-humidity environment. The average elasticity data of the volunteers at different time intervals are obtained by calculation, and the results are shown in table 6.

TABLE 6

Time	D0	D14	D28
				Application example 1	0.62	0.65	0.89
Application example 2	0.61	0.68	1.07
				Application example 3	0.60	0.66	0.90
Application example 4	0.59	0.62	0.84
				Application example 5	0.57	0.59	0.80
Application example 6	0.53	0.55	0.71
				Application example 7	0.54	0.55	0.73
Application example 8	0.55	0.56	0.72
				Application example 9	0.56	0.56	0.70
Application example 10	0.56	0.54	0.67
				Application example 11	0.54	0.56	0.72
Application example 12	0.55	0.53	0.67
				Application comparative example 1	0.52	0.55	0.68
Comparative application example 2	0.50	0.53	0.67
				Comparative application example 3	0.51	0.53	0.55
Application comparative example 4	0.49	0.52	0.52
				Comparative application example 5	0.51	0.49	0.60
Comparative application example 6	0.45	0.52	0.55
				Application comparative example 7	0.49	0.50	0.54
Comparative application example 8	0.50	0.50	0.56
				Comparative application 9	0.50	0.51	0.51
Comparative application example 10	0.52	0.55	0.57
				Comparative application example 11	0.35	0.40	0.492

The experiments show that the moisturizing essence prepared in the application examples 1-12 has a good moisturizing effect on skin compared with the application comparative examples 1-11, the elasticity of the skin of each experimental group using the moisturizing essence disclosed by the invention is obviously improved after the moisturizing essence is used for a period of time, and scientific moisturizing schemes are customized for different levels of a microecological layer, a stratum corneum, a granular layer, a spinous layer and a basal layer according to the moisturizing requirements of each layer of the epidermis of the skin from the side, so that the effect of deep moisturizing is achieved, the elasticity of the skin is enhanced, and the problem of the body skin is fundamentally improved.

The applicant states that the moisturizing compound, the moisturizing essence and the preparation method thereof containing the symbiotic bacteria combined fermentation product of the present invention are illustrated by the above examples, but the present invention is not limited to the above examples, i.e., it does not mean that the present invention must be implemented by the above examples. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.

Claims

1. A moisturizing complex comprising a fermentation product of a symbiotic bacteria combination, said moisturizing complex comprising: symbiotic bacteria combined fermentation product, white truffle extract, rhizobium glue, saccharide isomerate, hyaluronic silanol, medium molecular sodium hyaluronate, small molecular sodium hyaluronate, micro molecular sodium hyaluronate, ceramide 3 and beta-glucan;

the symbiotic bacteria of the symbiotic bacteria combined fermentation product comprise a combination of schizophyllum commune, thermus thermophilus, saccharomycetes and lactobacillus, and the fermentation raw materials of the symbiotic bacteria combined fermentation product comprise rice, Sichuan millet seeds, ginseng, kelp and raspberry;

the mass ratio of schizophyllum commune, thermus thermophilus, microzyme and lactobacillus in the symbiotic bacteria is (8-18): 4-8): 2-6): 1-3;

the mass ratio of the rice, the Sichuan millet seeds, the ginseng, the kelp and the raspberry in the fermentation raw materials is (40-60): (40-60):1: (20-30): 20-30);

the preparation method of the symbiotic bacteria combined fermentation product comprises the following steps:

2. The moisturizing compound comprising symbiotic composite fermentate according to claim 1, wherein the moisturizing compound comprises, in parts by weight: 1-10 parts of symbiotic bacteria combined leavening, 0.01-5 parts of truffle extract, 0.01-5 parts of rhizobium glue, 0.01-5 parts of saccharide isomer, 0.01-5 parts of hyaluronic silanol, 0.01-5 parts of medium molecular sodium hyaluronate, 0.01-5 parts of small molecular sodium hyaluronate, 0.01-5 parts of micro molecular sodium hyaluronate, 30.1-10 parts of ceramide and 0.1-10 parts of beta-glucan.

3. The moisturizing compound comprising symbiotic composite fermentate according to claim 1, wherein the sum of the mass of the symbiotic bacteria is 20-30% of the sum of the mass of the fermentation raw materials.

4. The moisturizing complex comprising a symbiotic combined ferment of claim 1, wherein the hyaluronan silanol is comprised of a macromolecular hyaluronic acid and a silanetriol.

5. The moisturizing composite containing symbiotic composite fermented product according to claim 4, wherein the mass ratio of the macromolecular hyaluronic acid to the silanetriol is (2-4) to (6-8).

6. The moisturizing complex comprising a fermentation broth of a symbiotic composition according to claim 4, wherein the molecular weight of the hyaluronan is 150-180 kilodaltons.

7. The moisturizing complex comprising symbiotic composite fermentate of claim 1 wherein the medium molecule sodium hyaluronate has a molecular weight of 80 to 100 kilodaltons.

8. The moisturizing complex comprising symbiotic composite fermentate of claim 1 wherein the small molecule acetylated sodium hyaluronate has a molecular weight of 20 to 40 kilodaltons.

9. The moisturizing complex comprising symbiotic composite fermentate of claim 1 wherein the micro-molecules of sodium hyaluronate have a molecular weight of less than 1 kilodalton.

10. A moisturizing essence, characterized in that the moisturizing essence comprises the moisturizing compound containing the symbiotic bacteria combined ferment as claimed in any one of claims 1 to 9.

11. The moisturizing essence according to claim 10, wherein the moisturizing essence comprises the following components in percentage by mass:

5-20% of a moisturizing complex comprising a symbiotic bacterial composition ferment of any one of claims 1-9, 1-30% of a humectant, 5-20% of an emollient, 1-10% of an emulsifier and 0.5-2% of a skin conditioner, the balance being rose water.

12. The moisturizing essence of claim 11, wherein the moisturizer comprises any one or a combination of at least two of 1, 2-hexanediol, butylene glycol, glycerin, panthenol, xylitol, anhydroxylitol, or xylitol-based glucoside.

13. The moisturizing essence of claim 11, wherein the emollient comprises any one or a combination of at least two of squalane, canola oil, caprylic/capric triglyceride, polydimethylsiloxane, or stearic acid.

14. The moisturizing essence of claim 11, wherein the emulsifier comprises any one or a combination of at least two of cetearyl alcohol, cetearyl glucoside, polyglyceryl-3-methylglucdistearate, glyceryl stearate, PEG-100 stearate, sodium acrylate copolymer, or lecithin.

15. The moisturizing essence of claim 11, wherein the skin conditioner comprises any one or a combination of at least two of dipotassium glycyrrhizinate, hydrogenated lecithin, a flos buddlejae extract, a radix sophorae flavescentis extract, a radix glycyrrhizae inflatae extract, a radix scutellariae extract, or a narcissus bulb extract.

16. The moisturizing essence of claim 15, wherein the skin conditioner is a combination of dipotassium glycyrrhizinate, hydrogenated lecithin, a flos buddlejae extract, a radix sophorae flavescentis extract, a radix glycyrrhizae inflatae extract, a radix scutellariae extract, and a narcissus bulb extract.

17. The moisturizing essence of claim 11, wherein the moisturizing essence further comprises 0.02-0.1% of a chelating agent.

18. The moisturizing essence of claim 17, wherein the chelating agent is disodium EDTA.

19. The moisturizing essence of claim 11, wherein the moisturizing essence further comprises 0.01-0.1% of a thickener.

20. The moisturizing essence of claim 19, wherein the thickener is hydrolyzed sclerotium rolfsii gum.

21. The moisturizing essence of claim 11, wherein the moisturizing essence further comprises 0.5-2% of an antioxidant.

22. The moisturizing essence of claim 21, wherein the antioxidant is p-hydroxyacetophenone and/or tocopherol acetate.

23. The preparation method of the moisturizing essence of any one of claims 11 to 16, wherein the preparation method comprises the following steps:

(3) adding the moisturizing compound containing the symbiotic bacteria combination fermentation product and the skin conditioner into the homogenized liquid obtained in the step (2), and mixing and stirring to obtain the moisturizing essence.

24. The preparation method of the moisturizing essence as claimed in claim 23, wherein the moisturizing essence further comprises a chelating agent, a thickener and an antioxidant, and the preparation method comprises the following steps:

25. The preparation method of the moisturizing essence as claimed in claim 23 or 24, wherein the temperature of the water phase mixing in the step (1) is 80-85 ℃, and the time of the water phase mixing is 5-10 min.

26. The preparation method of the moisturizing essence as claimed in claim 23 or 24, wherein the temperature of the oil phase mixing in the step (1) is 78-80 ℃, and the time of the oil phase mixing is 5-10 min.

27. The preparation method of the moisturizing essence as claimed in claim 23 or 24, wherein the temperature of the mixing and homogenizing in the step (2) is 80-82 ℃, and the time of the mixing and homogenizing is 5-10 min.

28. The preparation method of the moisturizing essence as claimed in claim 23 or 24, wherein the mixing and stirring temperature in the step (3) is 40-50 ℃, and the mixing and stirring time is 5-10 min.

29. The preparation method of the moisturizing essence as claimed in claim 24, wherein the preparation method comprises the following steps:

(3) adding the moisturizing compound containing the symbiotic bacteria combination fermentation product and the skin conditioner into the homogenized liquid obtained in the step (2), and mixing and homogenizing at 40-50 ℃ for 5-10min to obtain the moisturizing essence.