CN111133099B - 神经纤毛蛋白-1（nrp1）作为细胞表面标志物用于分离人心脏心室祖细胞的用途 - Google Patents

神经纤毛蛋白-1（nrp1）作为细胞表面标志物用于分离人心脏心室祖细胞的用途 Download PDF

Info

Publication number
CN111133099B
CN111133099B CN201880054988.6A CN201880054988A CN111133099B CN 111133099 B CN111133099 B CN 111133099B CN 201880054988 A CN201880054988 A CN 201880054988A CN 111133099 B CN111133099 B CN 111133099B
Authority
CN
China
Prior art keywords
cells
human
nrp1
progenitor cells
cardiac
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201880054988.6A
Other languages
English (en)
Chinese (zh)
Other versions
CN111133099A (zh
Inventor
K·R·钱
J·克拉克
C·Y·梁
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Prosera Therapy
Original Assignee
Prosera Therapy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prosera Therapy filed Critical Prosera Therapy
Publication of CN111133099A publication Critical patent/CN111133099A/zh
Application granted granted Critical
Publication of CN111133099B publication Critical patent/CN111133099B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/035—Animal model for multifactorial diseases
    • A01K2267/0375—Animal model for cardiovascular diseases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/98—Xeno-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Plant Pathology (AREA)
  • Cardiology (AREA)
  • Rheumatology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Animal Husbandry (AREA)
CN201880054988.6A 2017-08-23 2018-08-22 神经纤毛蛋白-1（nrp1）作为细胞表面标志物用于分离人心脏心室祖细胞的用途 Active CN111133099B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762549345P 2017-08-23 2017-08-23
US62/549,345 2017-08-23
PCT/IB2018/001026 WO2019038587A1 (en) 2017-08-23 2018-08-22 USE OF NEUROPILIN-1 (NRP1) AS A CELL SURFACE MARKER FOR ISOLATING HUMAN CARDIAC VENTRICULAR PROGENITOR CELLS

Publications (2)

Publication Number Publication Date
CN111133099A CN111133099A (zh) 2020-05-08
CN111133099B true CN111133099B (zh) 2023-12-01

Family

ID=63794530

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201880054988.6A Active CN111133099B (zh) 2017-08-23 2018-08-22 神经纤毛蛋白-1（nrp1）作为细胞表面标志物用于分离人心脏心室祖细胞的用途

Country Status (7)

Country Link
US (2) US11186820B2 (OSRAM)
EP (2) EP3487988B1 (OSRAM)
JP (2) JP7275109B2 (OSRAM)
CN (1) CN111133099B (OSRAM)
CA (1) CA3072579A1 (OSRAM)
DK (1) DK3487988T3 (OSRAM)
WO (1) WO2019038587A1 (OSRAM)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10596200B2 (en) 2014-08-22 2020-03-24 Procella Therapeutics Ab Use of LIFR or FGFR3 as a cell surface marker for isolating human cardiac ventricular progenitor cells
WO2017172086A1 (en) 2016-02-19 2017-10-05 Leung Chuen Yan Genetic markers for engraftment of human cardiac ventricular progenitor cells
US10508263B2 (en) 2016-11-29 2019-12-17 Procella Therapeutics Ab Methods for isolating human cardiac ventricular progenitor cells
WO2019038587A1 (en) 2017-08-23 2019-02-28 Procella Therapeutics Ab USE OF NEUROPILIN-1 (NRP1) AS A CELL SURFACE MARKER FOR ISOLATING HUMAN CARDIAC VENTRICULAR PROGENITOR CELLS
WO2021033699A1 (ja) 2019-08-20 2021-02-25 国立大学法人京都大学 心筋細胞の富化方法
CN113046308A (zh) * 2021-03-05 2021-06-29 苏州大学附属第二医院 促进心脏损伤后修复的一类新型成纤维细胞及其标识方法
WO2023278382A1 (en) 2021-06-29 2023-01-05 Staffan Holmin Endoluminal delivery cannula
US20230330264A1 (en) 2022-04-15 2023-10-19 Smartcella Solutions Ab COMPOSITIONS AND METHODS FOR EXOSOME-MEDIATED DELIVERY OF mRNA AGENTS
CN114752688B (zh) * 2022-04-28 2024-02-23 北京大学口腔医学院 鉴定人类胚胎骨髓来源的间充质干细胞的方法、探针和试剂盒及其用途
GB202314218D0 (en) 2023-09-18 2023-11-01 Astrazeneca Ab Cell therapy
CN120699894A (zh) * 2024-03-25 2025-09-26 中国科学院分子细胞科学卓越创新中心 Clusterin阳性心脏类器官及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104411331A (zh) * 2012-01-13 2015-03-11 哈佛学院董事会 在结构聚合装置中tlr激动剂的控制传递

Family Cites Families (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5561384A (en) 1995-11-08 1996-10-01 Advanced Micro Devices, Inc. Input/output driver circuit for isolating with minimal power consumption a peripheral component from a core section
US5994619A (en) 1996-04-01 1999-11-30 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Production of chimeric bovine or porcine animals using cultured inner cell mass cells
US5945577A (en) 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells
US7166280B2 (en) 2000-04-06 2007-01-23 Franco Wayne P Combination growth factor therapy and cell therapy for treatment of acute and chronic heart disease
EP1435977A4 (en) 2001-09-19 2005-06-08 Ford Henry Health System HEART TRANSPLANTATION OF STEM CELLS FOR THE TREATMENT OF HEART FAILURE
CA2514810A1 (en) 2003-01-31 2004-08-19 The Regents Of The University Of California Use of islet 1 as a marker for isolating or generating stem cells
DE602005012081D1 (de) 2005-07-29 2009-02-12 Univ Bonn Verfahren zur Isolierung von Stammzellen aus ein kissenformiges Gewebe der Zähne
US20080038229A1 (en) 2006-08-08 2008-02-14 Minguell Jose J Intracoronary injection of a mixture of autologous bone marrow derived mononuclear cells and autologous bone marrow derived mesenchymal stem cells for utilization and rescue of infarcted myocardium
US20100166714A1 (en) 2006-11-02 2010-07-01 The General Hospital Corporation Cardiovascular stem cells, methods for stem cell isolation, and uses thereof
WO2008058273A2 (en) 2006-11-09 2008-05-15 The Johns Hopkins University Dedifferentiation of adult mammalian cardiomyocytes into cardiac stem cells
US20080182328A1 (en) 2006-12-19 2008-07-31 The Burnham Institute Mammalian extraembryonic endoderm cells and methods of isolation
JP2010517578A (ja) 2007-02-09 2010-05-27 ザ ジェネラル ホスピタル コーポレイション Ｉｓｌｅｔ１＋系統に入るように細胞を誘導する方法およびそれを拡大する方法
WO2009017460A1 (en) 2007-07-31 2009-02-05 Es Cell International Pte Ltd Method for identifying and selecting cardiomyocytes
EP2245140A2 (en) 2007-11-30 2010-11-03 New York Medical College Compositions comprising hdac inhibitors and methods of their use in restoring stem cell function and preventing heart failure
EP2238162A1 (en) * 2008-01-24 2010-10-13 The Government of the United States of America as represented by The Secretary of the Department of Health and Human Services Induced internalization of surface receptors
WO2010042856A2 (en) 2008-10-09 2010-04-15 The General Hospital Corporation Tissue engineered myocardium and methods of production and uses thereof
WO2010144678A2 (en) 2009-06-10 2010-12-16 The General Hospital Corporation Generation of vascularized human heart tissue and uses thereof
EP2494032A4 (en) * 2009-10-29 2013-06-05 Univ Mcmaster GENERATION OF INDUCED PLURIPOTENTER STEM CELLS AND FIBROBLAST PRECURSOR CELLS
WO2011091945A1 (en) * 2010-01-26 2011-08-04 Université Libre de Bruxelles Tools for isolating and following cardiovascular progenitor cells
US20130209415A1 (en) 2010-06-03 2013-08-15 The Board Of Trustees Of The Leland Stanford Junior University Purified compositions of cardiovascular progenitor cells
US20120009158A1 (en) 2010-06-18 2012-01-12 The General Hospital Corporation VENTRICULAR INDUCED PLURIPOTENT STEM (ViPS) CELLS FOR GENERATION OF AUTOLOGOUS VENTRICULAR CARDIOMYOCYTES AND USES THEREOF
WO2012006503A1 (en) * 2010-07-09 2012-01-12 Genentech, Inc. Anti-neuropilin antibodies and methods of use
AU2010100830A4 (en) 2010-08-03 2010-09-16 Robert Fallshaw Biased Pin Lock
WO2012070014A2 (en) 2010-11-26 2012-05-31 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Identification of novel cell surface markers for pancreatic progenitor cells and definite endodermal cells
EP2647384B1 (en) 2010-12-02 2018-05-23 Riken IMMUNOTHERAPY USING ALLO-NKT CELLS, CELLS FOR IMMUNOTHERAPY IN WHICH alpha CHAIN OF T-CELL RECEPTOR (TCR) GENE HAS BEEN REARRANGED TO UNIFORM V alpha-J alpha , AND BANKING OF NKT CELLS DERIVED FROM SAID CELLS
CA2829804A1 (en) * 2011-03-29 2012-10-04 Geron Corporation Enriched populations of cardiomyocyte lineage cells from pluripotent stem cells
KR101323650B1 (ko) * 2011-05-04 2013-11-05 연세대학교 산학협력단 미분화 전능성 줄기세포의 제거방법
WO2012162740A1 (en) 2011-05-31 2012-12-06 Sonomedical Pty Ltd Electronic monitoring system for the production of data packages for analytic and diagnostic purposes
WO2012162741A1 (en) 2011-06-01 2012-12-06 Monash University Enrichment of cardiomyocytes
US8951798B2 (en) 2011-10-13 2015-02-10 Wisconsin Alumni Research Foundation Generation of cardiomyocytes from human pluripotent stem cells
JP5920741B2 (ja) 2012-03-15 2016-05-18 ｉＨｅａｒｔ Ｊａｐａｎ株式会社 人工多能性幹細胞から心筋および血管系混合細胞群を製造する方法
US9234176B2 (en) 2012-11-13 2016-01-12 The Board Of Trustees Of The Leland Stanford Junior University Chemically defined production of cardiomyocytes from pluripotent stem cells
WO2014150602A1 (en) 2013-03-15 2014-09-25 Fate Therapeutics, Inc. Cell potency assay for therapeutic potential
US20150079626A1 (en) 2013-03-19 2015-03-19 Lsip, Llc Method of obtaining a cell population containing cancer stem cells
US10888588B2 (en) * 2013-10-18 2021-01-12 Icahn School Of Medicine At Mount Sinai Directed cardiomyocyte differentiation and ventricular specification of stem cells
ES2689804T3 (es) 2013-11-20 2018-11-15 Miltenyi Biotec Gmbh Composiciones de subpoblaciones de cardiomiocitos
US9987310B2 (en) * 2013-11-27 2018-06-05 University Of Louisville Research Foundation, Inc. Cardiac progenitor cells and methods of use therefor
EP3105253B1 (en) 2014-02-12 2018-06-27 F. Hoffmann-La Roche AG Anti-jagged1 antibodies and methods of use
CA2940691C (en) 2014-03-11 2022-10-25 Indiana University Research And Technology Corporation Method for generating endothelial colony forming cell-like cells
WO2015166638A1 (ja) * 2014-05-01 2015-11-05 ｉＨｅａｒｔ Ｊａｐａｎ株式会社 Ｃｄ８２陽性心筋前駆細胞
US10144916B2 (en) * 2014-05-09 2018-12-04 Becton, Dickinson And Company Cell surface signature for processing cardiomyocyte subsets from heterogeneous cell samples
US10596200B2 (en) 2014-08-22 2020-03-24 Procella Therapeutics Ab Use of LIFR or FGFR3 as a cell surface marker for isolating human cardiac ventricular progenitor cells
DK3183337T3 (en) 2014-08-22 2019-04-01 Procella Therapeutics Ab Use of Jagged 1 / Frizzled 4 as cell surface marker for isolation of human cardiac ventricular progenitor cells
US9765299B2 (en) 2014-09-10 2017-09-19 Wisconsin Alumni Research Foundation Chemically defined albumin-free conditions for cardiomyocyte differentiation of human pluripotent stem cells
CN107108743B (zh) 2014-12-03 2021-05-04 (株)爱恩德生物 结合神经毡蛋白1的抗体及其用途
WO2016131137A1 (en) * 2015-02-17 2016-08-25 University Health Network Methods for making and using sinoatrial node-like pacemaker cardiomyocytes and ventricular-like cardiomyocytes
WO2017172086A1 (en) 2016-02-19 2017-10-05 Leung Chuen Yan Genetic markers for engraftment of human cardiac ventricular progenitor cells
US10508263B2 (en) 2016-11-29 2019-12-17 Procella Therapeutics Ab Methods for isolating human cardiac ventricular progenitor cells
WO2019038587A1 (en) 2017-08-23 2019-02-28 Procella Therapeutics Ab USE OF NEUROPILIN-1 (NRP1) AS A CELL SURFACE MARKER FOR ISOLATING HUMAN CARDIAC VENTRICULAR PROGENITOR CELLS

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104411331A (zh) * 2012-01-13 2015-03-11 哈佛学院董事会 在结构聚合装置中tlr激动剂的控制传递

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Neural crest–derived SEMA3C activates endothelial NRP1 for cardiac outflow tract septation;Alice Plein等;The Journal of Clinical Investigation;第125卷(第125期);2661-2676 *
NRP1 function and targeting in neurovascular development and eye disease;Claudio Raimondi等;Progress in Retinal and Eye Research;第52卷;63-84 *
诱导多能干细胞和心脏干 /祖细胞对心肌修复作用的研究进展;曾春雨;解放军医药杂志;第24卷(第24期);1-4 *

Also Published As

Publication number Publication date
EP3487988A1 (en) 2019-05-29
JP2020531028A (ja) 2020-11-05
US11186820B2 (en) 2021-11-30
CA3072579A1 (en) 2019-02-28
JP2023093754A (ja) 2023-07-04
EP3663393A1 (en) 2020-06-10
WO2019038587A1 (en) 2019-02-28
JP7275109B2 (ja) 2023-05-17
CN111133099A (zh) 2020-05-08
DK3487988T3 (da) 2020-02-03
US20190062696A1 (en) 2019-02-28
EP3487988B1 (en) 2020-01-01
US20220112458A1 (en) 2022-04-14
US12404488B2 (en) 2025-09-02

Similar Documents

Publication Publication Date Title
JP7386290B2 (ja) ヒト心室前駆細胞の生着のための遺伝子マーカー
JP7275109B2 (ja) ニューロピリン－１（ｎｒｐ１）のヒト心室前駆細胞を単離するための細胞表面マーカーとしての使用
JP6738808B2 (ja) ヒト心室前駆体細胞を単離するための細胞表面マーカーとしてのＪａｇｇｅｄ１／Ｆｒｉｚｚｌｅｄ４の使用
US11401508B2 (en) Methods for isolating human cardiac ventricular progenitor cells
US20200268803A1 (en) Use of lifr or fgfr3 as a cell surface marker for isolating human cardiac ventricular progenitor cells
JP5371445B2 (ja) 脂肪組織由来細胞を培養する方法及びその使用
US11607429B2 (en) Derivation and self-renewal of ISI1+ cells and uses thereof
JP6989591B2 (ja) 多能性細胞の誘導及び自己再生並びにその使用
HK40022436A (en) Use of jagged 1/frizzled 4 as a cell surface marker for isolating human cardiac ventricular progenitor cells
US20250339470A1 (en) Methods and compositions for maturing cardiomyocytes
HK1232911B (en) Use of jagged 1/frizzled 4 as a cell surface marker for isolating human cardiac ventricular progenitor cells
HK1232911A1 (en) Use of jagged 1/frizzled 4 as a cell surface marker for isolating human cardiac ventricular progenitor cells
Subrahmanyam et al. Application of Stem Cells in Ischemic Heart Disease
IL193947A (en) Cells cultured as cd31brigi: it, a method for stimuli to differentiate into a congenital / precursor cell population (pcp), use of the same drug preparation population, and implantation device that includes it

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant