CN111109374A - 一种虾糜豆腐的制备方法 - Google Patents
一种虾糜豆腐的制备方法 Download PDFInfo
- Publication number
- CN111109374A CN111109374A CN202010049491.4A CN202010049491A CN111109374A CN 111109374 A CN111109374 A CN 111109374A CN 202010049491 A CN202010049491 A CN 202010049491A CN 111109374 A CN111109374 A CN 111109374A
- Authority
- CN
- China
- Prior art keywords
- minced
- shrimp
- bean curd
- soybean milk
- stirring
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000238557 Decapoda Species 0.000 title claims abstract description 72
- 235000013527 bean curd Nutrition 0.000 title claims abstract description 51
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 230000000694 effects Effects 0.000 claims abstract description 20
- 235000013372 meat Nutrition 0.000 claims abstract description 18
- 235000013305 food Nutrition 0.000 claims abstract description 14
- 238000004806 packaging method and process Methods 0.000 claims abstract description 12
- 238000010894 electron beam technology Methods 0.000 claims abstract description 7
- 230000001954 sterilising effect Effects 0.000 claims abstract description 6
- 244000068988 Glycine max Species 0.000 claims description 38
- 235000010469 Glycine max Nutrition 0.000 claims description 38
- 235000013336 milk Nutrition 0.000 claims description 34
- 239000008267 milk Substances 0.000 claims description 34
- 210000004080 milk Anatomy 0.000 claims description 34
- 238000003756 stirring Methods 0.000 claims description 30
- 241000238017 Astacoidea Species 0.000 claims description 24
- 239000004365 Protease Substances 0.000 claims description 24
- 238000010438 heat treatment Methods 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 108090000270 Ficain Proteins 0.000 claims description 10
- 235000019836 ficin Nutrition 0.000 claims description 10
- POTUGHMKJGOKRI-UHFFFAOYSA-N ficin Chemical compound FI=CI=N POTUGHMKJGOKRI-UHFFFAOYSA-N 0.000 claims description 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 9
- 239000002105 nanoparticle Substances 0.000 claims description 9
- 239000000047 product Substances 0.000 claims description 9
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 8
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 8
- 239000001630 malic acid Substances 0.000 claims description 8
- 235000011090 malic acid Nutrition 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 8
- 244000269722 Thea sinensis Species 0.000 claims description 7
- 150000008442 polyphenolic compounds Chemical class 0.000 claims description 7
- 235000013824 polyphenols Nutrition 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 6
- 238000005520 cutting process Methods 0.000 claims description 6
- 230000003187 abdominal effect Effects 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 235000011962 puddings Nutrition 0.000 claims description 4
- 239000011265 semifinished product Substances 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 4
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 4
- UIIMBOGNXHQVGW-UHFFFAOYSA-M sodium bicarbonate Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 4
- 229920001661 Chitosan Polymers 0.000 claims description 3
- 229920002261 Corn starch Polymers 0.000 claims description 3
- 239000008120 corn starch Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 2
- 238000011049 filling Methods 0.000 claims description 2
- 235000015110 jellies Nutrition 0.000 claims description 2
- 239000008274 jelly Substances 0.000 claims description 2
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 abstract description 28
- 238000000034 method Methods 0.000 abstract description 14
- 238000006722 reduction reaction Methods 0.000 abstract description 10
- 238000000586 desensitisation Methods 0.000 abstract description 9
- 238000012545 processing Methods 0.000 abstract description 7
- 238000001784 detoxification Methods 0.000 abstract description 6
- 230000001717 pathogenic effect Effects 0.000 abstract description 4
- 239000005022 packaging material Substances 0.000 abstract description 2
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 2
- 230000002035 prolonged effect Effects 0.000 abstract 1
- 239000003053 toxin Substances 0.000 description 9
- 231100000765 toxin Toxicity 0.000 description 9
- 108700012359 toxins Proteins 0.000 description 9
- 108091005804 Peptidases Proteins 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 4
- 108090000526 Papain Proteins 0.000 description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 241000191967 Staphylococcus aureus Species 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 235000019834 papain Nutrition 0.000 description 4
- 229940055729 papain Drugs 0.000 description 4
- 235000019419 proteases Nutrition 0.000 description 4
- 108010004032 Bromelains Proteins 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 3
- 102000035195 Peptidases Human genes 0.000 description 3
- 102000004142 Trypsin Human genes 0.000 description 3
- 108090000631 Trypsin Proteins 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 239000013566 allergen Substances 0.000 description 3
- 235000019835 bromelain Nutrition 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000003672 processing method Methods 0.000 description 3
- 239000012588 trypsin Substances 0.000 description 3
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 2
- 241000242759 Actiniaria Species 0.000 description 2
- 241001083548 Anemone Species 0.000 description 2
- 201000005569 Gout Diseases 0.000 description 2
- 102000003505 Myosin Human genes 0.000 description 2
- 108060008487 Myosin Proteins 0.000 description 2
- 239000004809 Teflon Substances 0.000 description 2
- 229920006362 Teflon® Polymers 0.000 description 2
- 230000002009 allergenic effect Effects 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000013357 binding ELISA Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 239000003797 essential amino acid Substances 0.000 description 2
- 235000020776 essential amino acid Nutrition 0.000 description 2
- 108010083391 glycinin Proteins 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
- 210000000936 intestine Anatomy 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 238000004537 pulping Methods 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 238000008157 ELISA kit Methods 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 206010039020 Rhabdomyolysis Diseases 0.000 description 1
- 241000767840 Semiaquilegia Species 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 1
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000037208 balanced nutrition Effects 0.000 description 1
- 235000019046 balanced nutrition Nutrition 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 239000003975 dentin desensitizing agent Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000004280 healthy diet Nutrition 0.000 description 1
- 230000001071 malnutrition Effects 0.000 description 1
- 235000000824 malnutrition Nutrition 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 208000015380 nutritional deficiency disease Diseases 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000021404 traditional food Nutrition 0.000 description 1
- 229940116269 uric acid Drugs 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 229940075420 xanthine Drugs 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C20/00—Cheese substitutes
- A23C20/02—Cheese substitutes containing neither milk components, nor caseinate, nor lactose, as sources of fats, proteins or carbohydrates
- A23C20/025—Cheese substitutes containing neither milk components, nor caseinate, nor lactose, as sources of fats, proteins or carbohydrates mainly containing proteins from pulses or oilseeds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/40—Shell-fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/26—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by irradiation without heating
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/25—Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
- C08J5/18—Manufacture of films or sheets
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
- C08J2305/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2403/00—Characterised by the use of starch, amylose or amylopectin or of their derivatives or degradation products
- C08J2403/02—Starch; Degradation products thereof, e.g. dextrin
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K3/00—Use of inorganic substances as compounding ingredients
- C08K3/02—Elements
- C08K3/08—Metals
- C08K2003/0806—Silver
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K3/00—Use of inorganic substances as compounding ingredients
- C08K3/18—Oxygen-containing compounds, e.g. metal carbonyls
- C08K3/20—Oxides; Hydroxides
- C08K3/22—Oxides; Hydroxides of metals
- C08K2003/2296—Oxides; Hydroxides of metals of zinc
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K2201/00—Specific properties of additives
- C08K2201/011—Nanostructured additives
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Manufacturing & Machinery (AREA)
- Microbiology (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
本发明属于食品加工领域,具体公开了一种虾糜豆腐的制备方法,本发明为首次在食品加工领域关注到小龙虾类海葵毒素的致病风险,并为之采取酸碱脱毒方式处理以消除影响,有效降低虾肉致病风险;在降嘌呤,脱毒和脱敏的处理效果上,效果稳定,非常适用于食品工业;在包装时采用杀菌包装材料和电子束技术联用方式,有效延长低温保存的虾糜豆腐货架期,制备出的虾糜豆腐鲜嫩可口,老少皆宜,非常适合产业化。
Description
技术领域
本发明属于食品加工领域,具体涉及一种虾糜豆腐的制备方法。
背景技术
小龙虾(克氏原螯虾)味道鲜美,营养丰富。虾球肌肉中含18种氨基酸,其中包括8种必需氨基酸,是其主要食用部位。随着稻田养虾模式在我国中南部省份的大规模推广,小龙虾2019年的全国总产量已突破163万吨。由于小龙虾上市时间集中(高峰期5月至8月),加工消费方式单一(以餐饮为主),造成养殖农户增产不增收,甚至虾贱伤农情况时有发生,小龙虾的加工利用方式急需研究拓展。同时,小龙虾嘌呤含量较高,不适合高尿酸消费者和痛风患者食用;每年吃虾旺季爆出的“食用小龙虾引发横纹肌溶解症”等新闻报道也引发了消费者对其食用安全性的担忧。
豆腐是我国的传统美食,因其植物蛋白含量丰富,低糖低脂低胆固醇,非常符合现代生活提倡的健康饮食标准,已经随中餐出海扩散被全世界消费者认知和接受。但是豆腐与小龙虾一样,存在可能引发人体过敏的异源蛋白,且都属于高嘌呤易引发痛风的食物。另外,虽然豆腐蛋白含量丰富,却缺乏蛋氨酸等人体必需氨基酸,若将传统豆腐作为长期单一蛋白质来源存在营养不良风险。
发明内容
针对上述问题,本发明的目的在于提供了一种虾糜豆腐的制备方法,本发明将虾糜与豆腐混合在一起,完善了食品中的氨基酸的种类,同时该方法很好的处理了虾糜豆腐中可能存在的致敏,高嘌呤和小龙虾毒素的问题,利用该方法制备的虾糜豆腐味道鲜美,食用安全,适合大规模推广。
为了实现上述目的,本发明采取以下技术措施:
本发明的发明思路为:申请人针对背景技术中提到的问题,首次想到通过酸碱脱毒的方式来消除小龙虾类海葵毒素的影响,从而降低小龙虾潜在的食用风险,同时,申请人在处理脱敏问题时,发现目前公开的脱敏试剂存在脱敏效果不稳定的情况,在食品领域,效果稳定非常重要,这直接影响到食品安全性,因此,本发明需要探索出一种处理效果稳定的食品加工方案。
一种虾糜豆腐的制备方法,包括下述步骤:
(1)取小龙虾腹部虾肉;
(2)取10kg虾肉搅碎后,先加入100~300ml苹果酸溶液搅拌5~10min,再加入NaHCO3溶液150~230ml继续搅拌5~10min,得到虾糜备用;
所述的苹果酸溶液的质量浓度为50~60%,NaHCO3溶液的质量浓度为40~55%;
(3)5~8kg大豆洗净后浸泡,制成豆浆并过滤得到生豆浆;
(4)生豆浆加热得到熟豆浆,冷却至60~70℃;
(5)将步骤(1)得到的虾糜加入熟豆浆中,再加入无花果蛋白酶180~300g(食品级,酶活≥5.0×105U/g)后,维持60~70℃,真空搅拌机270~360rpm搅拌30~40min;
(6)将虾糜豆浆混合物降温至28~35℃,加入茶多酚80~130g,真空搅拌机280~350rpm搅拌10~15min;
(7)将虾糜豆浆混合物温度升至37~45℃,加入1~2.5%(质量分数)的老浆,搅拌混合均匀后出现豆花,加热灭酶活;
所述的老浆为豆腐水发酵而成;
(8)将豆花倒入成型模具内,加热得到虾糜豆腐半成品。
(9)将半成品虾糜豆腐切块,装纳米保鲜包装袋抽真空后电子束灭菌,辐照剂量为8~10kGy,束能流量为9~12MeV;
所述的纳米保鲜包装袋的制备方法包括:
1-2.5克壳聚糖粉末加97.5-99克乙酸混合,向该溶液中加入终浓度为0.25-1%的玉米淀粉并充分搅拌;然后添加Ag纳米粒子和ZnO纳米粒子使其终浓度均为10-30ppm,然后搅拌均匀,将该溶液倒在不粘板上,通风干燥,将所得薄膜粘贴挤压成包装袋即得。
与现有技术相比,本发明具有以下优点:
1.本发明提供一种口味鲜美,营养均衡,安全可靠的虾糜豆腐加工方法。经过添加茶多酚降嘌呤、酸碱脱毒和添加无花果蛋白酶脱敏处理后的虾糜豆腐,适合煎炒烹炸蒸煮等各种烹饪方式,保质期也远超普通盒装豆腐。市场推广前景十分广阔,极具推广价值。
2.本发明为首次在食品加工领域关注到小龙虾类海葵毒素的致病风险,并为之采取酸碱脱毒方式处理以消除影响,有效降低虾肉致病风险。
3.本发明在包装时采用杀菌包装材料和电子束技术联用方式,有效延长低温保存的虾糜豆腐货架期,制备出的虾糜豆腐鲜嫩可口,营养丰富。
4.本发明的制备方法,在降嘌呤,脱毒和脱敏的处理效果上,效果稳定,非常适用于食品工业。
附图说明
图1为添加无花果蛋白酶的虾糜豆腐与对照组相比的致敏源消除效果图。
图2为酸碱法脱除虾糜类海葵毒素效果示意图。
图3为茶多酚对虾糜豆腐中的嘌呤去除效果示意图。
图4为不同的包装方法的抑菌保鲜效果图。
具体实施方式
本发明所述技术方案,如未特别说明,均为本领域的常规方案;所述试剂或材料,如未特别说明,均来源于商业渠道。
实施例1:
脱敏试剂的筛选:
本研究先后选用了菠萝蛋白酶、木瓜蛋白酶、无花果酶和胰蛋白酶等数种食品工业中的常用蛋白酶,通过间接ELISA法检测IgE结合能力反映各种蛋白酶对本产品的致敏源消除效果。
以未添加蛋白酶的虾糜豆腐匀浆液为实验材料,每50ml匀浆液加入2g上述4种蛋白酶中的1种,每种蛋白酶各设3个平行。加热搅拌30min,菠萝蛋白酶、木瓜蛋白酶、无花果酶和胰蛋白酶保持反应温度分别为55℃、58℃、65℃、37℃。然后将各反应匀浆液释至2.5μg/mL,在酶标板上每孔加入100μL。按照试剂盒(Sigma-Aldrich小鼠IgE ELISA试剂盒,RAB0799)说明书完成加抗体血清、震荡混匀、孵育、洗板、及显色剂等操作步骤,测定每个各加样孔在450nm下的吸光度(OD)。
未添加蛋白酶的虾糜豆腐即为,在制备过程中不添加蛋白酶,但其余制备方法同实施例2制备的虾糜豆腐。
其中菠萝蛋白酶和胰蛋白酶脱敏效果较差(IgE结合ELISA吸光度OD>0.8),木瓜蛋白酶脱敏效果稳定性较差(IgE结合ELISA吸光度0.3<OD<0.75)。无花果酶脱敏效果较好,且稳定性明显优于木瓜蛋白酶。
实施例2:
一种虾糜豆腐的加工方法,包括下述步骤:
(1)小龙虾在循环过滤池中清水暂养12h,水温维持30℃,水中添加3‰的NaCl和4‰的苹果酸,待小龙虾排净肠内污物后,剪取腹部虾肉。
(2)取10kg虾肉纯水清洗后放入真空搅拌机内,250rpm搅拌10min,以酸碱法消除虾肉中可能存在的类海葵毒素:先加入100ml苹果酸溶液(质量浓度50%)继续搅拌5min,再加入NaHCO3溶液150ml(质量浓度40%)继续搅拌5min,得到虾糜备用。
(3)精选5kg大豆,洗净后5倍以上体积的清水中浸泡10h,然后加入磨浆机中制成豆浆并过滤得到生豆浆。
(4)生豆浆加热至95℃保持5分钟,降温至70℃,得到熟豆浆。
(5)将虾糜加入熟豆浆中,再加入无花果蛋白酶200g后,维持65℃真空搅拌机300rpm搅拌30min,达到虾肉中的肌球蛋白和豆浆中的伴大豆球蛋白、大豆球蛋白等致敏性异源蛋白脱敏目的。
(6)将虾糜豆浆混合物降温至28℃,加入茶多酚80g,真空搅拌机300rpm搅拌10min,达到脱除虾肉和豆浆中嘌呤的目的。
(7)将虾糜豆浆混合物温度升至40℃,加入1%的老浆,搅拌混合均匀15min后出现豆花,加热至95℃灭酶活;所述的老浆为豆腐水发酵而成;
(8)将豆花倒入120目网底方形容器(体积40L),静置30min排出多余浆水后倒入成型模具内,加热至95℃保持10分钟,得到虾糜豆腐半成品。
(9)将半成品虾糜豆腐送入切块机,切成20×10×5cm的长方体(1L体积),装纳米保鲜包装袋抽真空后电子束灭菌,辐照剂量为8kGy,束能流量为9MeV。
纳米保鲜包装袋制备方法:
1克壳聚糖粉末加99克乙酸混合,向该溶液中加入终浓度为0.25%(质量百分比)的玉米淀粉并充分搅拌;然后添加Ag和ZnO纳米粒子使其终浓度均为10ppm,然后搅拌2h。将该溶液倒入涂有特氟龙涂层的不粘板上,并置于室温下。随后,将特氟龙板在烘箱中55℃通风干燥过夜,然后剥离。所得薄膜粘贴挤压成包装袋,并储存在干燥器中备用。
以上方法重复5次,然后检测产品指标。
实施例3:
一种虾糜豆腐的加工方法,包括下述步骤:
(1)小龙虾在循环过滤池中清水暂养12h,水温维持30℃,水中添加4‰的NaCl和5‰的苹果酸,待小龙虾排净肠内污物后,剪取腹部虾肉。
(2)取10kg虾肉纯水清洗后放入真空搅拌机内,250rpm搅拌10min,以酸碱法消除虾肉中可能存在的类海葵毒素:先加入200ml苹果酸溶液(质量浓度50%)继续搅拌5min,再加入NaHCO3溶液300ml(质量浓度40%)继续搅拌5min,得到虾糜备用。
(3)精选5kg大豆,洗净后5倍以上体积的清水中浸泡10h,然后加入磨浆机中制成豆浆并过滤得到生豆浆。
(4)生豆浆加热至95℃保持5分钟,降温至65℃,得到熟豆浆。
(5)将虾糜加入熟豆浆中,再加入无花果蛋白酶300g后,维持65℃真空搅拌机300rpm搅拌30min,达到虾肉中的肌球蛋白和豆浆中的伴大豆球蛋白、大豆球蛋白等致敏性异源蛋白脱敏目的。
(6)将虾糜豆浆混合物降温至28℃,加入茶多酚120g,真空搅拌机300rpm搅拌10min,达到脱除虾肉和豆浆中嘌呤的目的。
(7)将虾糜豆浆混合物温度升至40℃,加入1%的老浆,搅拌混合均匀15min后出现豆花,加热至95℃灭酶活,所述的老浆为豆腐水发酵而成;
(8)将豆花倒入120目网底方形容器(体积40L),静置30min排出多余浆水后倒入成型模具内,加热至100℃保持10分钟,得到虾糜豆腐半成品。
(9)将半成品虾糜豆腐送入切块机,切成20×10×5cm的长方体(1L体积),装纳米保鲜包装袋抽真空后电子束灭菌,辐照剂量为10kGy,束能流量为12MeV。
以上方法重复5次,然后检测产品指标。
实施例4:
产品质量检测
通过间接ELISA法检测IgE结合能力反映本产品的致敏源消除效果。以各实施例中制备得到的虾糜豆腐匀浆液稀释至2.5μg/mL,在酶标板上每孔加入100μL,以未添加无花果蛋白酶的虾糜豆腐匀浆液为对照,如图1所示,对照组OD=0.97±0.11,实施例2的OD=0.42±0.06,实施例3的OD=0.28±0.03。
以岩沙海葵毒素为验证材料,采用多抗直接ELISA法检测酸碱法脱除类海葵毒素效果。向各实施例中虾糜加入岩沙海葵毒素(100μg/kg);
所述的虾糜是实施例2或3中第(2)步得到的虾糜。
以未经酸碱法处理的虾糜为对照,如图2所示,对照组OD=0.88±0.16,实施例2的OD=0.25±0.03,实施例3的OD=0.13±0.02。
以各实施例中虾糜豆腐为验证材料,以未加茶多酚的虾糜豆腐为对照,采用反相液相色谱法测定最终产品中的嘌呤脱除效果(四种主要嘌呤物质:腺嘌呤、鸟嘌呤、黄嘌呤和次黄嘌呤),如图3所示,对照组嘌呤含量=11.5±1.33,实施例2的嘌呤含量=3.6±0.30,实施例3的嘌呤含量=2.2±0.40。
以各实施例中最终得到的虾糜豆腐成品为检测对象,以普通PVC保鲜膜包装且未经电子束辐照杀菌的产品为对照,0~4℃冷藏保存30天后测定了金黄色葡萄球菌和大肠杆菌对数减少值,如图4所示,对照组大肠杆菌对数减少值=1.85,金黄色葡萄球菌对数减少值=1.62;实施例2的大肠杆菌对数减少值=0.35,金黄色葡萄球菌对数减少值=0.41;实施例3的大肠杆菌对数减少值=0.29,金黄色葡萄球菌对数减少值=0.53。
Claims (1)
1.一种虾糜豆腐的制备方法,包括下述步骤:
(1)取小龙虾腹部虾肉;
(2)取10kg虾肉搅碎后,先加入100~300ml苹果酸溶液搅拌5~10min,再加入NaHCO3溶液150~230ml继续搅拌5~10min,得到虾糜备用;
所述的苹果酸溶液的质量浓度为50~60%,NaHCO3溶液的质量浓度为40~55%;
(3)5~8kg大豆洗净后浸泡,制成豆浆并过滤得到生豆浆;
(4)生豆浆加热得到熟豆浆,冷却至60~70℃;
(5)将步骤(1)得到的虾糜加入熟豆浆中,再加入无花果蛋白酶180~300g(食品级,酶活≥5.0×105U/g)后,维持60~70℃,真空搅拌机270~360rpm搅拌30~40min;
(6)将虾糜豆浆混合物降温至28~35℃,加入茶多酚80~130g,真空搅拌机280~350rpm搅拌10~15min;
(7)将虾糜豆浆混合物温度升至37~45℃,加入1~2.5%(质量分数)的老浆,搅拌混合均匀后出现豆花,加热灭酶活;
所述的老浆为豆腐水发酵而成;
(8)将豆花倒入成型模具内,加热得到虾糜豆腐半成品;
(9)将半成品虾糜豆腐切块,装纳米保鲜包装袋抽真空后电子束灭菌,辐照剂量为8~10kGy,束能流量为9~12MeV;
所述的纳米保鲜包装袋的制备方法包括:
1-2.5克壳聚糖粉末加97.5-99克乙酸混合,向该溶液中加入终浓度为0.25-1%的玉米淀粉并充分搅拌;然后添加Ag纳米粒子和ZnO纳米粒子使其终浓度均为10-30ppm,然后搅拌均匀,将该溶液倒在不粘板上,通风干燥,将所得薄膜粘贴挤压成包装袋即得。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010049491.4A CN111109374B (zh) | 2020-01-16 | 2020-01-16 | 一种虾糜豆腐的制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010049491.4A CN111109374B (zh) | 2020-01-16 | 2020-01-16 | 一种虾糜豆腐的制备方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN111109374A true CN111109374A (zh) | 2020-05-08 |
CN111109374B CN111109374B (zh) | 2022-09-09 |
Family
ID=70490092
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202010049491.4A Active CN111109374B (zh) | 2020-01-16 | 2020-01-16 | 一种虾糜豆腐的制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN111109374B (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114698785B (zh) * | 2022-03-21 | 2023-06-30 | 杭州九阳豆业有限公司 | 增加饮品中虾青素含量的方法及富含虾青素的豆浆饮品 |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4820529A (en) * | 1986-06-26 | 1989-04-11 | Asahi Denka Kogyo Kabushiki Kaisha | Process for preparing pasty proteinous material or proteinous food from crustaceans |
CN103159970A (zh) * | 2013-04-09 | 2013-06-19 | 河南工业大学 | 一种具有抗菌和抗氧化功能的可食性膜的制备方法 |
CN104106662A (zh) * | 2014-06-23 | 2014-10-22 | 马鞍山江心绿洲食品有限公司 | 一种葱香虾仁豆干及其制备方法 |
CN104585337A (zh) * | 2015-01-09 | 2015-05-06 | 马鞍山市黄池食品(集团)有限公司 | 一种蒜香虾仁卤豆干及其制备方法 |
CN105815780A (zh) * | 2016-04-03 | 2016-08-03 | 徐俊 | 一种草本膳食虾肉粉丝及其制备方法 |
CN105831277A (zh) * | 2016-04-26 | 2016-08-10 | 黄树娟 | 一种虾仁温补花生豆腐及其制备方法 |
CN106172734A (zh) * | 2016-07-27 | 2016-12-07 | 四川农业大学 | 一种草莓保鲜复合包装膜及其制备方法 |
-
2020
- 2020-01-16 CN CN202010049491.4A patent/CN111109374B/zh active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4820529A (en) * | 1986-06-26 | 1989-04-11 | Asahi Denka Kogyo Kabushiki Kaisha | Process for preparing pasty proteinous material or proteinous food from crustaceans |
CN103159970A (zh) * | 2013-04-09 | 2013-06-19 | 河南工业大学 | 一种具有抗菌和抗氧化功能的可食性膜的制备方法 |
CN104106662A (zh) * | 2014-06-23 | 2014-10-22 | 马鞍山江心绿洲食品有限公司 | 一种葱香虾仁豆干及其制备方法 |
CN104585337A (zh) * | 2015-01-09 | 2015-05-06 | 马鞍山市黄池食品(集团)有限公司 | 一种蒜香虾仁卤豆干及其制备方法 |
CN105815780A (zh) * | 2016-04-03 | 2016-08-03 | 徐俊 | 一种草本膳食虾肉粉丝及其制备方法 |
CN105831277A (zh) * | 2016-04-26 | 2016-08-10 | 黄树娟 | 一种虾仁温补花生豆腐及其制备方法 |
CN106172734A (zh) * | 2016-07-27 | 2016-12-07 | 四川农业大学 | 一种草莓保鲜复合包装膜及其制备方法 |
Non-Patent Citations (6)
Title |
---|
姜竹茂: "《食品添加剂实用手册》", 30 November 2004, 中国农业出版社 * |
孙佳益: "南美白对虾主要过敏原原肌球蛋白的过敏原性消减方法探讨", 《上海海洋大学第十四届研究生学术论文报告会论文集》 * |
张愍等: "《水产类调理食品加工过程品质调控理论与实践》", 31 January 2013, 中国医药科技出版社 * |
张汀等: "《电子束辐照技术在检疫处理中的应用》", 31 March 2013, 中国质检出版社 * |
王光慈: "《食品营养学》", 30 June 2001, 中国农业出版社 * |
王明晓等: "《户外医学》", 31 July 2010, 人民军医出版社 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114698785B (zh) * | 2022-03-21 | 2023-06-30 | 杭州九阳豆业有限公司 | 增加饮品中虾青素含量的方法及富含虾青素的豆浆饮品 |
Also Published As
Publication number | Publication date |
---|---|
CN111109374B (zh) | 2022-09-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN105661377B (zh) | 一种高蛋白低热量鱼肉脆片的制备方法 | |
CN104171387A (zh) | 一种防病治病母猪饲料及其制作方法 | |
CN104351811A (zh) | 一种禽肉粒的制备方法 | |
CN104789622B (zh) | 一种低致敏性低腥味鱼蛋白肽全粉及其工业化制备方法和应用 | |
KR20140141317A (ko) | 황태 보리 고추 된장의 제조 방법 및 황태 보리 고추 된장 | |
CN111202231B (zh) | 用于制备酱牛肉的营养骨汤、酱牛肉及其制备方法 | |
CN111109374B (zh) | 一种虾糜豆腐的制备方法 | |
CN112553033A (zh) | 一种保肝大鲵肝肽酒配方及其生产工艺 | |
CN101830747B (zh) | 一种西藏亚东野生黑木耳人工栽培的培养料 | |
CN105192316A (zh) | 一种改良肉质的改性梨渣饲料添加剂及其制备方法 | |
CN105707843A (zh) | 复合生物酶解生产贻贝酱的方法 | |
RU2455859C2 (ru) | Способ получения белково-витаминно-минерального концентрата | |
CN106579064B (zh) | 一种增强铁吸收的鱼肉休闲食品及其制备方法 | |
CN105146153A (zh) | 一种藏香猪母猪孕期配方饲料 | |
CN111557416A (zh) | 一种大麦若叶鱼脯及其制备方法 | |
CN104686927A (zh) | 蘑菇杀青水制备高钙纤体芸豆面条的方法 | |
CN108813168A (zh) | 一种以桑枝为主的豚狸饲料及其制备方法 | |
CN110214860A (zh) | 一种宠物饲料及其制备工艺 | |
CN110810810A (zh) | 一种香菇西瓜酱及其制备方法 | |
CN111374239A (zh) | 一种鳝鱼用营养饲料及其制备方法 | |
CN109907292A (zh) | 一种富含蛋白质和氨基酸的海鲜调味酱油及其制备方法 | |
CN113812547B (zh) | 一种促进蜜蜂蜂群增长的饲料及其制备方法 | |
KR101180444B1 (ko) | 옥수수를 이용한 고추장의 제조방법 | |
CN109770232B (zh) | 一种华蒜豆食品及其制备方法 | |
RU2326552C1 (ru) | Способ производства мясного формованного продукта с детоксикационными свойствами для питания детей школьного возраста |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |