CN111035597A

CN111035597A - Bacteriostatic itching-relieving topless drops and preparation method thereof

Info

Publication number: CN111035597A
Application number: CN202010026796.3A
Authority: CN
Inventors: 郑生华; 林铭敏; 周林贵; 陈家绍; 罗文诗; 古雪霞
Original assignee: Guangzhou Baby Mum Biotechnology Co ltd
Current assignee: Guangzhou Baby Mum Biotechnology Co ltd
Priority date: 2020-01-10
Filing date: 2020-01-10
Publication date: 2020-04-21

Abstract

The invention relates to bacteriostatic antipruritic raticide, which is prepared from the following raw materials in parts by weight: 23.5-73.07 parts of phase A component; 26.9-73.5 parts of phase B component; 0.03-3 parts of phase C component; wherein: the phase A component consists of water and EDTA-2 Na; the phase B component comprises SP115C, propylene glycol, Mentholum, ethanol, Borneolum Syntheticum, flos Matricariae Chamomillae extract, flos Malloti Apeltae extract, radix Paeoniae extract, flos Lonicerae extract, herba Taraxaci extract, herba Violae extract and herba Avenae Fatuae alkaloid; the phase C component consists of caprylyl hydroximic acid, ethylhexyl glycerol and 1, 2-hexanediol; its preparing process is also disclosed. The invention has the advantages that: the combination of various plant extracts has good effects of repairing, diminishing inflammation and resisting allergy, and the discomfort caused by pruritus after mosquito bites is relieved; the medicinal properties of the medicinal materials are matched with each other, so that the effects of repelling mosquitoes, relieving itching, diminishing inflammation and easing pain can be achieved; the raw materials are fully utilized, the cost is reduced, the economic effect is improved, and the popularization is convenient.

Description

Bacteriostatic itching-relieving topless drops and preparation method thereof

Technical Field

The invention relates to the technical field of daily chemical industry, in particular to bacteriostatic itching-relieving topless drops and a preparation method thereof.

Background

In spring and summer, along with the return of the warm climate, a great amount of mosquitoes are bred, and the probability that people are bitten by the mosquitoes is obviously increased. When mosquitoes bite a human body, saliva in salivary glands of the mosquitoes is injected into the skin, and the saliva contains various anticoagulant chemical substances, so that ecchymosis appears on the skin of a bite part; meanwhile, saliva of mosquitoes contains allergy-related proteins such as salivary allergens, natural antigen proteins and adenosine deaminase, and the substances cause local or systemic allergic reactions after a human body is bitten. This dermatitis caused by mosquito bites is called insect bite dermatitis, and is a common condition in dermatology in summer.

The dermatitis caused by insect bite is usually characterized in that pimples, wheal, edematous erythema, blisters, herpes dunalis, ecchymosis of petechiae and the like appear at the local part of the bite, the center of the skin injury can be seen with bite marks, and people feel stabbing pain, burning pain and extreme itching with different degrees, wherein the most common skin pruritus is caused by anaphylactic reaction of the bite part of a person caused by mosquito bite, the skin pruritus caused by mosquito bite, and the traditional Chinese medicine considers that poisonous juice invades the skin, hurts collaterals, and the collaterals are stagnant, and the qi and blood circulation is not smooth, and the stagnation is caused by heat. The main pathological changes are toxicity, stasis and heat. The treatment is mainly based on clearing away heat and toxic material, removing blood stasis and dredging collaterals, and is assisted by expelling wind and arresting itching. The pruritus causes unpleasant physiological and psychological feelings, and brings trouble to the life of people.

The common topical preparation for repelling mosquito and relieving itching on skin comprises ointment, cream, gel, spray, etc. Most of the mosquito repellent incense has the defects of large smell and inconvenient application, and the special mosquito repellent smell of the mosquito repellent incense causes unpleasant feeling to some people.

The mosquito repellent liquid on the market is various in types, and due to the fact that the mosquito repellent liquid contains various chemical components, although the itching relieving effect is good, adverse reactions of the skin, such as skin allergy, rough skin, pigmentation and the like are easily induced. In recent years, some products are added with relatively effective plant extracts, but a single plant extract has short mosquito repelling time and unsatisfactory itching relieving effect. Therefore, the research and development of a product combining bacteriostasis and itching relieving are in line with the market demand.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provides a bacteriostatic itching-relieving topknot drop and a preparation method thereof.

The purpose of the invention is realized by the following technical scheme:

the bacteriostatic itching-relieving topknot drop is prepared from the following raw materials in parts by weight:

23.5-73.07 parts of a phase A component;

26.9-73.5 parts of a phase B component;

0.03-3 parts of a phase C component;

wherein:

the phase A component consists of water and EDTA-2 Na;

the phase B component comprises SP115C, propylene glycol, Mentholum, ethanol, Borneolum Syntheticum, flos Matricariae Chamomillae extract, flos Malloti Apeltae extract, radix Paeoniae extract, flos Lonicerae extract, herba Taraxaci extract, herba Violae extract and herba Avenae Fatuae alkaloid;

the phase C component consists of caprylyl hydroximic acid, ethylhexyl glycerol and 1, 2-hexanediol.

Further, the phase A component comprises the following raw materials in parts by weight:

23.49-73.02 parts of water;

0.01-0.05 part of EDTA-2 Na.

Further, the phase B component comprises the following raw materials in parts by weight:

further, the phase C component comprises the following raw materials in parts by weight:

0.01-1 part of caprylyl hydroximic acid;

0.01-1 part of ethylhexyl glycerol;

0.01-1 part of 1, 2-hexanediol.

Furthermore, in the phase A component, the water is deionized water.

The preparation method of the bacteriostatic antipruritic raticide comprises the following steps:

s1, heating the water in the phase A component to 80-90 ℃, sterilizing at a constant temperature for 20-40 min, and adding EDTA-2 Na;

s2, mutually dispersing and solubilizing all components of the phase B component;

s3, cooling the phase A component to 40-45 ℃, adding the phase B component obtained in the step S2 into the phase A component obtained in the step S1, and uniformly stirring;

s4, adding the phase C component after uniformly stirring, and stirring and mixing for 3-5 min;

and S5, detecting to be qualified, and filtering and discharging the material by using a filter screen.

Further, in step S1, the specific operation steps of heating are: firstly, heating the water phase pot, pouring water into the water phase pot, keeping the temperature for 20-40 min after the temperature reaches 80-85 ℃.

Further, in step S2, the menthol and borneol are solubilized by the solubilizer, and then the other components of the phase B component are added.

Further, in step S3, the cooling rate is 0.4-0.8 ℃/min, and during stirring, a forward and reverse rotation alternative stirring method is adopted, wherein the stirring speed is 20-30 rpm.

The invention has the following advantages:

1. by adopting the antibacterial antipruritic Bidding drops and the preparation method thereof, and combining with various plant extracts, the anti-inflammatory and anti-allergic anti-pruritus drop has good repairing, anti-inflammatory and anti-allergy effects, and relieves the discomfort caused by pruritus after mosquito bites. The medicinal materials are matched with each other to achieve the effects of repelling mosquitoes, relieving itching, diminishing inflammation and easing pain.

2. When the traditional Chinese medicine composition is used in summer, the traditional Chinese medicine composition has an effect of preventing mosquitoes, can play a role in relieving itching and pain and can quickly reduce swelling when being used after being bitten by mosquitoes. The raw materials are fully utilized, the cost is reduced, the economic effect is improved, and the popularization is convenient.

3. The invention belongs to a solution preparation form and a homogeneous liquid preparation, has no problem of physical stability, has simple preparation process, is convenient for industrial production, and has the advantages of convenient administration, high comfort level, good patient compliance, no pollution to clothes and the like.

Detailed Description

The invention is further described below with reference to examples, but the scope of the invention is not limited to the following.

[ example 1 ]:

23.5 parts of a phase A component;

73.5 parts of phase B component;

3 parts of a phase C component;

wherein:

the phase A component consists of water and EDTA-2 Na;

23.49 parts of water;

0.01 part of EDTA-2 Na.

1 part of caprylyl hydroximic acid;

1 part of ethylhexyl glycerol;

1 part of 1, 2-hexanediol.

Furthermore, in the phase A component, the water is deionized water.

s1, heating the water in the phase A component to 80 ℃, sterilizing at constant temperature for 40min, and adding EDTA-2 Na;

s3, cooling the phase A component to 40 ℃, adding the phase B component obtained in the step S2 into the phase A component obtained in the step S1, and uniformly stirring;

s4, adding the phase C component after uniformly stirring, and stirring and mixing for 5 min;

In this embodiment, in step S1, the specific operation steps of heating are: heating the water phase pot, pouring water into the water phase pot, keeping the temperature for 40min after the temperature reaches 80 ℃.

In this example, in step S2, the menthol and borneol are first solubilized by the solubilizer, and then the other components of the phase B component are added.

In this example, in step S3, the cooling rate was 0.4 ℃/min, and during stirring, a forward-reverse alternate stirring method was employed, with a stirring rate of 30 rpm.

[ example 2 ]:

48 parts of phase A component;

phase B component 50.5;

1.5 parts of phase C component;

wherein:

the phase A component consists of water and EDTA-2 Na;

47.97 parts of water;

0.03 part of EDTA-2 Na.

0.5 part of caprylyl hydroximic acid;

0.5 part of ethylhexyl glycerin;

0.5 part of 1, 2-hexanediol.

Furthermore, in the phase A component, the water is deionized water.

s1, heating the water in the phase A component to 5 ℃, sterilizing at constant temperature for 30min, and adding EDTA-2 Na;

s3, cooling the phase A component to 42 ℃, adding the phase B component obtained in the step S2 into the phase A component obtained in the step S1, and uniformly stirring;

s4, adding the phase C component after uniformly stirring, and stirring and mixing for 4 min;

In this embodiment, in step S1, the specific operation steps of heating are: heating the water phase pot, pouring water into the water phase pot, keeping the temperature for 30min after the temperature reaches 82 ℃.

In this example, in step S3, the cooling rate was 0.6 ℃/min, and during stirring, a forward-reverse alternate stirring method was employed, with a stirring rate of 25 rpm.

[ example 3 ]:

50 parts of phase A component;

48 parts of phase B component;

2 parts of phase C component;

wherein:

the phase A component consists of water and EDTA-2 Na;

49.96 parts of water;

0.04 part of EDTA-2 Na.

0.06 part of caprylyl hydroximic acid;

0.06 part of ethylhexyl glycerol;

0.08 part of 1, 2-hexanediol.

Furthermore, in the phase A component, the water is deionized water.

s1, heating the water in the phase A component to 82 ℃, sterilizing at constant temperature for 28min, and adding EDTA-2 Na;

s3, cooling the phase A component to 43 ℃, adding the phase B component obtained in the step S2 into the phase A component obtained in the step S1, and uniformly stirring;

In this embodiment, in step S1, the specific operation steps of heating are: heating the water phase pot, pouring water into the water phase pot, keeping the temperature for 33min after the temperature reaches 84 ℃.

In this example, in step S3, the cooling rate was 0.5 ℃/min, and during stirring, a forward-reverse alternate stirring method was employed, with a stirring rate of 27 rpm.

Example 4:

73.07 parts of phase A component;

26.9 parts of a phase B component;

phase C component 0.03 part;

wherein:

the phase A component consists of water and EDTA-2 Na;

73.02 parts of water;

0.05 part of EDTA-2 Na.

0.01 part of caprylyl hydroximic acid;

0.01 part of ethylhexyl glycerin;

0.01 part of 1, 2-hexanediol.

Furthermore, in the phase A component, the water is deionized water.

s1, heating the water in the phase A component to 90 ℃, sterilizing at constant temperature for 20min, and adding EDTA-2 Na;

s3, cooling the phase A component to 45 ℃, adding the phase B component obtained in the step S2 into the phase A component obtained in the step S1, and uniformly stirring;

s4, adding the phase C component after uniformly stirring, and stirring and mixing for 3 min;

In this embodiment, in step S1, the specific operation steps of heating are: heating the water phase pot, pouring water into the water phase pot, keeping the temperature for 20min after the temperature reaches 85 ℃.

In this example, in step S3, the cooling rate was 0.8 ℃/min, and during stirring, a forward-reverse alternate stirring method was employed, with a stirring rate of 20 rpm.

Some raw material sources and types in the above application examples are shown in table 1:

table 1:

effect testing

1. Antimicrobial Activity test

a, preparing a liquid medicine:

the test agents were formulated with sterile distilled water to a series of 5 concentrations with gradient. Sterile distilled water was used as a control.

b, preparing a test bacterium suspension:

pouring 10mL of sterilized distilled water on the cultured staphylococcus aureus strains, lightly scraping the planar spore suspension by using an inoculating needle, pouring the planar spore suspension into a sterilized triangular flask (a plurality of glass beads are filled in advance), shaking for 5min, and filtering the spore suspension into another sterilized triangular flask by using a sterilized double-layer gauze. The spore concentration is adjusted by microscopic examination at low magnification (15 x 20 times), preferably 80-100 spores per field. The above operations should be performed under aseptic conditions, and the actions should be rapid and accurate. Pouring a double-layer culture medium: 10mL of sterilized clear agar medium contained in a test tube was dissolved, poured into a 9 cm-diameter petri dish while it was hot, and horizontally condensed. Melting 100mL of culture medium suitable for the growth and development of test bacteria, cooling to about 45-50 ℃, quickly sucking 10mL of bacterial liquid, adding the bacterial liquid into the culture medium, fully and uniformly mixing, immediately sucking 5mL of culture medium with bacteria, adding the culture medium on the solidified clear water agar culture medium, and uniformly spreading the culture medium on the bottom layer.

c, medicine injection:

clamping stainless steel small tubes (i.e. Oxford cup, 8.0mm of outer diameter, 6.0mm of inner diameter and 10mm of height) by using a pair of sterilized tweezers, placing 6 stainless steel small tubes in each dish at proper intervals, wherein I small tubes are used as a control, and the other 5 tubes are 5 liquid medicines with different concentrations (the liquid medicines are added to form convex surfaces at the tube openings), and culturing at proper temperature.

d, checking the result:

after culturing for 10min, taking out and measuring the diameter of the inhibition zone by a cross method, and evaluating the bactericidal activity according to the existence and the size of the inhibition zone. If the diameter of the inhibition zone is elliptic, the difference of the length is more than one unit, and the inhibition zone is preferably left out.

e repeating the test for three species.

The results are shown in the following table:

2. test for antipruritic Effect

The experimental environment is as follows: the number of the volunteers is 50, the people are 2-50 years old, and the male and the female are all individuals which attract mosquito bites and local severe pruritus after the bites.

And (4) judging the standard: no itching feeling within 1 minute.

The application method is that the bacteriostatic antipruritic raticide prepared in the embodiment 2 of the invention is intensively sprayed on the part bitten by the mosquito, and the spraying times can be properly adjusted according to the size of the lumps on the part bitten by the mosquito.

The results of the experiment are as follows:

experimental results prove that after the mosquito bites the rapid itching relieving liquid prepared by the invention, 100 percent of people have itching relieving effect and can completely relieve itching within 10 minutes. The earlier the mosquito bites, the better the effect is, the itching can be quickly relieved, and no bite marks are left due to scratching.

In conclusion, the prepared antibacterial itching-relieving liquid has no specific drop or essence, is cool and comfortable, has good itching-relieving effect, is quick in inflammation diminishing and itching relieving, free of stimulation and long in effective time, adopts multiple medicinal materials to be matched with each other, has no toxic or side effect on a body, is safe and reliable to use, is cheap and good, is convenient to use and the like, realizes the adverse effect of quickly eliminating mosquito bites, and is suitable for all people to use.

Researches find that the effective components of the following plants have the effects of inhibiting bacteria and diminishing inflammation and are beneficial to reducing red, swollen, itchy and painful caused by being bitten by mosquitoes.

The mallow occidentalis is natural and moisture-retaining, has the functions of coordinating skin and helping to clean old waste cutin, and is added into cosmetics to enable the skin to be pure, fine and tender, so that the sensitive skin is relieved, and the pressure is relieved.

Radix Paeoniae, the effective component is paeoniflorin, and has inhibitory effect on pathogenic bacteria and fungi. Can achieve the bacteriostatic effect.

The flos Lonicerae contains luteolin with antibacterial and antiinflammatory effects.

The dandelion rhizome and root also have anti-inflammatory and antibacterial effects.

Viola yedoensis Makino has the effects of clearing heat and removing toxicity, cooling blood and relieving swelling, and clearing heat and promoting diuresis.

The flos Matricariae Chamomillae, i.e. flos Matricariae Chamomillae, contains volatile oil, flavonoid, amino acids, chlorogenic acid and microelements, etc., and the flos Chrysanthemi extract has biological activities of resisting bacteria, resisting oxidation, diminishing inflammation, relieving and resisting allergy, etc.

Avenanthramides, which are effective ingredients in oats, have been studied to show that the synthesized dihydroxy avenanthramide-D can reduce skin diseases such as itch, erythema, and water sore caused by histamine change, and have been used as an active ingredient of cosmetics.

In addition, menthol, also known as menthol, can selectively act on the skin's cold receptors when topically applied to produce a cold reflex that causes cutaneous vasoconstriction, and has cooling and antipruritic effects. Mentholum is cool and pungent in flavor, has effects of killing parasites and relieving itching, and can be used for treating skin pruritus. Wherein the L-menthol has a cooling effect. The research shows that the menthol has the function of promoting penetration of skin. The menthol can diminish inflammation, relieve pain, relieve itching, promote blood circulation, relieve edema and the like when externally used, in addition, the menthol is a good penetration enhancer and has better capability of promoting the percutaneous absorption of the medicine in various solvent systems.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The bacteriostatic antipruritic raticide is characterized in that: the feed is prepared from the following raw materials in parts by weight:

23.5-73.07 parts of a phase A component;

26.9-73.5 parts of a phase B component;

0.03-3 parts of a phase C component;

wherein:

the phase A component consists of water and EDTA-2 Na;

2. A bacteriostatic antipruritic raticide according to claim 1, wherein: the phase A component comprises the following raw materials in parts by weight:

23.49-73.02 parts of water;

0.01-0.05 part of EDTA-2 Na.

3. A bacteriostatic antipruritic raticide according to claim 2, wherein: the phase B component comprises the following raw materials in parts by weight:

4. a bacteriostatic antipruritic raticide according to claim 3, wherein: the phase C component comprises the following raw materials in parts by weight:

0.01-1 part of caprylyl hydroximic acid;

0.01-1 part of ethylhexyl glycerol;

0.01-1 part of 1, 2-hexanediol.

5. A bacteriostatic antipruritic raticide according to claim 2, wherein: in the phase A component, the water is deionized water.

6. The method for preparing bacteriostatic antipruritic Biandong according to any one of claims 1 to 5, which is characterized in that: the method comprises the following steps:

7. The method for preparing bacteriostatic antipruritic raticide according to claim 6, which is characterized in that: in step S1, the specific operation steps of heating are: firstly, heating the water phase pot, pouring water into the water phase pot, keeping the temperature for 20-40 min after the temperature reaches 80-85 ℃.

8. The method for preparing bacteriostatic antipruritic raticide according to claim 6, which is characterized in that: in step S2, the menthol crystal and borneol are solubilized by the solubilizer, and then the other components of the phase B component are added.

9. The method for preparing bacteriostatic antipruritic raticide according to claim 6, which is characterized in that: in step S3, the cooling speed is 0.4-0.8 ℃/min, and a forward and reverse alternate stirring method is adopted during stirring, wherein the stirring speed is 20-30 rpm.