CN111013690A - Centrifuging tube electromagnetic force support - Google Patents
Centrifuging tube electromagnetic force support Download PDFInfo
- Publication number
- CN111013690A CN111013690A CN202010082464.7A CN202010082464A CN111013690A CN 111013690 A CN111013690 A CN 111013690A CN 202010082464 A CN202010082464 A CN 202010082464A CN 111013690 A CN111013690 A CN 111013690A
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- Prior art keywords
- support
- lower transverse
- fixing hole
- transverse magnetic
- soft iron
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/06—Test-tube stands; Test-tube holders
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/20—Mixing the contents of independent containers, e.g. test tubes
- B01F31/201—Holders therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/20—Mixing the contents of independent containers, e.g. test tubes
- B01F31/27—Mixing the contents of independent containers, e.g. test tubes the vibrations being caused by electromagnets
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Electromagnetism (AREA)
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Centrifugal Separators (AREA)
Abstract
The invention discloses an electromagnetic force support of a centrifugal tube, which comprises a vertical support, an upper transverse support, an upper fixing hole, a lower transverse magnetic support, a lower fixing hole, an electromagnetic device, a switch and a power socket, wherein the vertical support is composed of two square plates with the same size, the upper transverse support is of a rectangular plate structure, the upper fixing hole is formed by uniformly distributing 12 small holes on the upper transverse support, the lower transverse magnetic support is composed of rectangular soft iron plates, the lower fixing hole is formed by uniformly distributing 12 small holes on the lower transverse magnetic support and corresponds to the upper fixing hole one by one, a soft iron cylinder is arranged below the center of the lower transverse magnetic support, and the electromagnetic device is composed of a copper enameled coil and a soft iron cylinder below the lower transverse magnetic support. The invention can control the magnetic beads by controlling the existence of magnetic force at any time by utilizing the principle of the electromagnet, realizes the function of batch washing and can greatly improve the working efficiency.
Description
Technical Field
The invention belongs to the technical field of molecular biology, and mainly relates to an electromagnetic force bracket of a centrifugal tube.
Background
The magnetic bead method for extracting nucleic acid can be generally divided into four steps: cracking, combining, washing and eluting. The magnetic bead method for extracting nucleic acid is to crack sample cells by using a cell lysis solution, nucleic acid molecules dissociated from the cells are specifically adsorbed to the surfaces of the magnetic beads to form magnetic bead-nucleic acid polymers, and impurities such as protein and the like are not adsorbed and remain in the solution. After reacting for a certain time, under the action of a magnetic field, separating the magnetic bead-nucleic acid polymer from the liquid, recovering the magnetic bead-nucleic acid polymer, and then eluting with an eluent to obtain pure nucleic acid. The magnetic bead method does not need to add various reagents and centrifugation, and is an important direction for developing a future nucleic acid purification method. The magnetic bead method needs to use a PCR tube magnetic frame, the existing PCR tube magnetic frame is of a structure that a permanent magnet block and a support are integrated, although the magnetic bead-nucleic acid polymer can be rapidly gathered, in the washing and elution process, the magnetic bead-nucleic acid polymer cannot be dispersed due to the permanent magnetic effect of a magnet, and the PCR tubes on the PCR tube magnetic frame need to be taken down one by one and shaken to enable the magnetic bead-nucleic acid polymer to obtain washing liquid or eluent, which is very inconvenient. Therefore, a magnetic frame of a PCR tube capable of regulating and controlling a magnetic field is very needed at present.
Disclosure of Invention
The invention aims to provide an electromagnetic force bracket of a centrifugal tube, which can effectively, quickly and safely finish a nucleic acid extraction test.
Therefore, the invention provides an electromagnetic force bracket of a centrifugal tube, which comprises a vertical bracket, an upper transverse bracket, an upper fixing hole, a lower transverse magnetic bracket, a lower fixing hole, an electromagnetic device, a switch and a power socket, wherein the vertical bracket is composed of two square plates with the same size, the upper transverse bracket is of a rectangular plate structure, the upper fixing hole is formed by uniformly distributing 12 small holes on the upper transverse bracket, the lower transverse magnetic bracket is composed of rectangular soft iron plates, the lower fixing hole is formed by uniformly distributing 12 small holes on the lower transverse magnetic bracket and corresponds to the upper fixing holes one by one, a soft iron cylinder is arranged below the center of the lower transverse magnetic bracket, the vertical bracket, the upper transverse bracket and the lower transverse magnetic bracket are fixed by upper transverse screws and are formed, and a certain distance is reserved between the upper transverse magnetic bracket and the lower transverse magnetic bracket, the electromagnetic device is composed of an enameled coil made of copper and a soft iron cylinder right below the lower transverse magnetic support, one end of the enameled coil of the electromagnetic device is connected with one end of a switch, the other end of the switch is connected with one end of a 12V direct-current power socket, and the other end of the enameled coil of the electromagnetic device is connected with the other end of the 12V direct-current power socket. The side length of the vertical support is 60-64mm, the thickness is 2-3mm, the length of the upper transverse support is 130-136mm, the width is 50-54mm, the thickness is 2-3mm, the diameter of the upper fixing hole is 10.5-11mm, the length of the lower transverse magnetic support is 130-136mm, the width is 50-54mm, the thickness is 2-3mm, the distance between the upper transverse support and the lower transverse magnetic support is 24-28mm, the diameter of the lower fixing hole is 4.8-5.0mm, the diameter of the soft iron cylinder below the center of the lower transverse magnetic support is 10-12mm, and the height is 15-17 mm. The vertical support and the upper horizontal support are made of aluminum alloy or medical plastic.
The principle of the invention is that the principle of the electromagnet is utilized, the existence of magnetic force can be controlled at any time, the control of magnetic beads is achieved, the batch washing effect is realized, and the working efficiency can be improved.
The technical scheme of the invention has the following advantages: the novel solar water heater has the advantages of small volume, light weight, simple structure, low price, safe and reliable use, great saving of manpower and material resources, environmental protection and capability of meeting market demands.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and claims hereof as well as the appended drawings.
The technical solution of the present invention is further described in detail by the accompanying drawings and embodiments.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention.
FIG. 1 is a schematic structural diagram of the present invention.
In the figure, 1 stands vertically; 2, an upper transverse bracket; 3, fixing holes are formed; 4, a lower transverse magnetic bracket; 5, lower fixing holes; 6 an electromagnetic device; 7, a switch; 8, a power socket; 9 soft iron cylinder.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
In the description of the present application, it is to be understood that the terms "intermediate," "top," "bottom," "inner," "outer," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the present application and simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed in a particular orientation, and be operated in a particular orientation, and thus should not be construed as limiting the present application. The terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.
In addition, unless expressly stated or limited otherwise, the terms "mounted," "connected," "secured," and the like are intended to be inclusive and mean, for example, that there may be a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.
In this application, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact of the first and second features, or may comprise contact of the first and second features not directly but through another feature in between. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
Referring to the figure, the invention provides an electromagnetic force bracket of a centrifugal tube, which comprises a vertical bracket 1, an upper horizontal bracket 2, an upper fixing hole 3, a lower horizontal magnetic bracket 4, a lower fixing hole 5, an electromagnetic device 6, a switch 7 and a power socket 8, wherein the vertical bracket 1 is composed of two square plates with the same size, the upper horizontal bracket 2 is in a rectangular plate structure, the upper fixing hole 3 is formed by uniformly distributing 12 small holes on the upper horizontal bracket 2, the lower horizontal magnetic bracket 4 is formed by rectangular soft iron plates, the lower fixing hole 5 is formed by uniformly distributing 12 small holes on the lower horizontal magnetic bracket 4 and corresponds to the upper fixing holes 3 one by one, a soft iron column 9 is arranged below the center of the lower horizontal magnetic bracket 4, the vertical bracket 1, the upper horizontal bracket 2 and the lower horizontal magnetic bracket 4 are formed by screw fixation, and a certain distance is reserved between the upper horizontal bracket 2 and the lower horizontal magnetic bracket 4, the electromagnetic device 6 is composed of an enameled coil made of copper and a soft iron cylinder 9 right below the lower transverse magnetic support 4, one end of the enameled coil of the electromagnetic device 6 is connected with one end of a switch 7, the other end of the switch is connected with one end of a 12V direct-current power socket 8, and the other end of the enameled coil of the electromagnetic device 6 is connected with the other end of the 12V direct-current power socket 8. The side length of the vertical support 1 is 60-64mm, the thickness is 2-3mm, the length of the upper transverse support 2 is 130-136mm, the width is 50-54mm, the thickness is 2-3mm, the diameter of the upper fixing hole 3 is 10.5-11mm, the length of the lower transverse magnetic support 4 is 130-136mm, the width is 50-54mm, the thickness is 2-3mm, the distance between the upper transverse support 2 and the lower transverse magnetic support 4 is 24-28mm, the diameter of the lower fixing hole 5 is 4.8-5.0mm, the diameter of the soft iron cylinder 9 at the center below the middle of the lower transverse magnetic support 4 is 10-12mm, and the height is 15-17 mm. The vertical support 1 and the upper transverse support 2 are made of aluminum alloy or medical plastic.
When the invention is used, firstly, an operator inserts a 12V direct current power supply into a power socket 8, then samples of nucleic acid to be extracted are respectively added into centrifuge tubes, the centrifuge tubes are respectively inserted into the upper fixing holes 3 of the upper transverse bracket 2 of the invention, the centrifuge tubes are fixed in the upper fixing holes 3 and the lower fixing holes 5, then lysate is respectively added, after 5-10 minutes, magnetic beads are respectively added, after 1-2 minutes, the invention is held by hands and slightly shaken or put on an oscillator to slightly shake the magnetic beads and the nucleic acid polymer to be fully combined to form the magnetic bead-nucleic acid polymer, at the moment, a switch 7 is opened, an electromagnetic device 6 generates magnetic force through a soft iron cylinder 9 and a lower transverse magnetic bracket 4, the magnetic bead-nucleic acid polymer is absorbed at the bottommost of the centrifuge tubes, at the moment, a sample adding device is used for absorbing liquid containing impurities such as protein and the like, then adding a washing solution, closing a switch 7, enabling an electromagnetic device 6 to disappear through the magnetic force generated by a soft iron cylinder 9 and a lower transverse magnetic force bracket 4, slightly shaking the magnetic bead-nucleic acid polymer gel electrophoresis device by holding the magnetic bead-nucleic acid polymer electrophoresis device by hands or placing the magnetic bead-nucleic acid polymer gel electrophoresis device on an oscillator to fully wash the nucleic acid sample liquid and fully combine the magnetic bead with the nucleic acid polymer to form the magnetic bead-nucleic acid polymer, opening the switch 7 again after 2-3 minutes, enabling the electromagnetic device 6 to generate the magnetic force through the soft iron cylinder 9 and the lower transverse magnetic force bracket 4 to adsorb the magnetic bead-nucleic acid polymer at the bottommost part of a centrifuge tube, then using a sample injector to suck the liquid containing the washing solution, then adding an eluent, closing the switch 7, enabling the electromagnetic device 6 to disappear through the magnetic force generated by the soft iron cylinder 9 and the lower transverse magnetic force bracket 4, slightly shaking the magnetic bead-nucleic acid polymer electrophoresis device to fully wash the magnetic bead-nucleic acid sample liquid and fully, make magnetism ball and nucleic acid polymer fully separate, open switch 7 again, electromagnetic means 6 produces magnetic force through soft iron cylinder 9 and lower horizontal magnetic support 4, adsorbs the magnetism ball at the bottommost of centrifuging tube, and the liquid that reuse application of sample ware will contain the eluant is siphoned away, and the eluant contains the nucleic acid in the sample this moment for nucleic acid detects.
The invention can control the existence of magnetic force at any time by utilizing the principle of the electromagnet, achieves the control of magnetic beads, realizes the function of batch washing, has small volume, light weight, simple structure, low price and safe and reliable use, greatly saves manpower and material resources, is green and environment-friendly, and meets the market demand.
It should be noted that the above embodiments are all preferred embodiments, relevant functional components may be replaced by other components, and the related units and modules are not necessarily required by the present application. The embodiments in the present specification are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The centrifuge tube electromagnetic force bracket provided by the application is described in detail above, and specific examples are applied in the description to explain the principle and the implementation of the application, and the description of the examples is only used to help understand the method and the core idea of the application; meanwhile, for a person skilled in the art, according to the idea of the present application, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present application.
Claims (3)
1. The utility model provides a centrifuging tube electromagnetic force support which characterized in that: the magnetic force sensor comprises a vertical support, an upper transverse support, an upper fixing hole, a lower transverse magnetic support, a lower fixing hole, an electromagnetic device, a switch and a power socket, wherein the vertical support is composed of two square plates with the same size, the upper transverse support is of a rectangular plate structure, 12 small holes are uniformly distributed in the upper transverse support, the lower transverse magnetic support is composed of rectangular soft iron plates, 12 small holes are uniformly distributed in the lower transverse magnetic support and correspond to the upper fixing hole one by one, a soft iron cylinder is arranged below the center of the lower transverse magnetic support, the vertical support, the upper transverse support and the lower transverse magnetic support are formed by fixing screws, a certain distance is reserved between the upper transverse support and the lower transverse magnetic support, and the electromagnetic device is composed of a copper enameled coil and a soft iron cylinder arranged right below the lower transverse magnetic support, one end of the enameled coil of the electromagnetic device is connected with one end of the switch, the other end of the switch is connected with one end of the 12V direct-current power socket, and the other end of the enameled coil of the electromagnetic device is connected with the other end of the 12V direct-current power socket.
2. The centrifuge tube electromagnetic force bracket of claim 1, which is characterized in that: the side length of the vertical support is 60-64mm, the thickness is 2-3mm, the length of the upper transverse support is 130-136mm, the width is 50-54mm, the thickness is 2-3mm, the diameter of the upper fixing hole is 10.5-11mm, the length of the lower transverse magnetic support is 130-136mm, the width is 50-54mm, the thickness is 2-3mm, the distance between the upper transverse support and the lower transverse magnetic support is 24-28mm, the diameter of the lower fixing hole is 4.8-5.0mm, the diameter of the soft iron cylinder below the center of the lower transverse magnetic support is 10-12mm, and the height is 15-17 mm.
3. The centrifuge tube electromagnetic force bracket of claim 1, which is characterized in that: the vertical support and the upper horizontal support are made of aluminum alloy or medical plastic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010082464.7A CN111013690A (en) | 2020-02-07 | 2020-02-07 | Centrifuging tube electromagnetic force support |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010082464.7A CN111013690A (en) | 2020-02-07 | 2020-02-07 | Centrifuging tube electromagnetic force support |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111013690A true CN111013690A (en) | 2020-04-17 |
Family
ID=70199202
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010082464.7A Pending CN111013690A (en) | 2020-02-07 | 2020-02-07 | Centrifuging tube electromagnetic force support |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111013690A (en) |
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2020
- 2020-02-07 CN CN202010082464.7A patent/CN111013690A/en active Pending
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20200417 |
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| WD01 | Invention patent application deemed withdrawn after publication |