CN211896992U - Magnetic force frame and magnetic force frame subassembly - Google Patents
Magnetic force frame and magnetic force frame subassembly Download PDFInfo
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- CN211896992U CN211896992U CN202020224246.8U CN202020224246U CN211896992U CN 211896992 U CN211896992 U CN 211896992U CN 202020224246 U CN202020224246 U CN 202020224246U CN 211896992 U CN211896992 U CN 211896992U
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Abstract
The utility model discloses a magnetic force frame and magnetic force frame subassembly, the magnetic force frame includes centrifugal tube sheet and the base that is located centrifugal tube sheet below to and the pillar of connecting centrifugal tube sheet and base, centrifugal tube sheet is provided with a plurality of centrifuging tube mounting holes, the base is provided with a plurality of centrifuging tube fixed orificess that correspond with the centrifuging tube mounting hole, the both sides of pillar are provided with multiunit magnetic sheet mounting hole, each magnetic sheet mounting hole of organizing includes the magnetic sheet mounting hole that two set up correspondingly, be provided with bearing structure in the magnetic sheet mounting hole, the magnetic sheet setting just supports and leans on in bearing structure in the magnetic sheet mounting hole, the magnetic sheet is located the outside of magnetic sheet mounting hole partially, make the centrifuging tube when inserting magnetic force frame, the surface and the centrifuging tube of magnetic sheet are laminated. The magnetic frame realizes the close contact of the magnetic sheets and the centrifuge tube by means of the exposed magnetic sheets and the supporting structure for supporting the magnetic sheets, enhances the magnetic field intensity in the centrifuge tube area, and saves the magnetic attraction time.
Description
Technical Field
The utility model belongs to the technical field of the bio-separation technique and specifically relates to a magnetic frame and magnetic frame subassembly for magnetic bead purification.
Background
Magnetic bead purification is a process of separating magnetic beads from proteins or nucleic acids by magnetic field separation to separate and purify substances such as cells, proteins or nucleic acids. The magnetic separation technology can rapidly separate nucleic acid, protein and the like from other substances in cells, has the characteristics of automation, simple operation, short time and the like, and has more and more obvious advantages compared with the traditional separation methods such as an extraction method, a precipitation method, a centrifugation method and the like. The magnetic bead method has the characteristics of high extraction efficiency, high separation speed, simple required equipment and the like.
Current magnetic frame generally comprises main part frame and magnetic sheet on the market, wherein, has seted up the centrifuging tube mounting hole on the main part frame, and the package buries magnet in the magnetic sheet. During the use, add the magnetic bead that can combine to wait to separate the sample in having the centrifuging tube of waiting to separate the sample, abundant mixing is hatched to place in centrifuging tube mounting hole on the magnetic frame, the magnetic bead in the sample can enrich on the centrifugal tube wall of magnetic plate one side under the magnetic field effect of magnet, then remove or pour the solution that does not adsorb, and then realize waiting to separate the separation of sample and other samples.
In the existing magnetic force frame, because the magnet is embedded in the magnetic plate, when the particle size is small, or the magnetic beads for purification are non-silicon hydroxyl magnetic beads with weaker adsorption capacity such as carboxyl magnetic beads and polystyrene magnetic beads, the distance between the magnet and the centrifugal tube and the magnetic field intensity are small, and more time is needed for waiting for magnetic attraction.
SUMMERY OF THE UTILITY MODEL
An object of the embodiment is to provide a magnetic force frame, especially strengthen the magnetic force frame of the regional magnetic field intensity of centrifuging tube on the one hand to wait for the long problem of magnetic absorption time in solving current magnetic force frame.
Some embodiments of this disclosure disclose a magnetic force frame, it includes centrifugal tube sheet and the base that is located centrifugal tube sheet below, and the pillar of connecting centrifugal tube sheet and base, centrifugal tube sheet is provided with a plurality of centrifuging tube mounting holes, the base is provided with a plurality of centrifuging tube fixed orificess that correspond with the centrifuging tube mounting hole, the both sides of pillar are provided with multiunit magnetic sheet mounting hole, each group magnetic sheet mounting hole includes the magnetic sheet mounting hole that two set up correspondingly, be provided with bearing structure in the magnetic sheet mounting hole, the magnetic sheet setting just leans on in bearing structure in the magnetic sheet mounting hole, the magnetic sheet is located the outside of magnetic sheet mounting hole partially, make when the centrifuging tube is inserting magnetic force frame, the surface and the centrifuging tube laminating of magnetic sheet.
Optionally, when the centrifuge tube is inserted into the magnetic rack, the magnetic sheet is attached to the centrifuge tube by fitting the centrifuge tube to the magnetic sheet.
Alternatively, the support structure is a boss or a cantilever provided on the bottom surface and/or the side wall of the magnetic sheet mounting hole.
Optionally, the support structure is proximate a lower portion of the magnet sheet mounting hole relative to an upper portion of the magnet sheet mounting hole.
Optionally, the magnetic sheet is rotatable relative to the support structure.
Further, the magnetic sheet is rotatable about the support structure under an external force applied to the magnetic sheet.
Optionally, the plane perpendicular to the pillar is a first cross section, and a projection of the magnetic sheet on the first cross section is partially overlapped with a projection of the centrifugal tube mounting hole on the first cross section.
Optionally, the two magnetic sheets respectively located in the correspondingly disposed magnetic sheet mounting holes are a group of magnetic sheets, and the magnetic poles of the two magnetic sheets in the group of magnetic sheets, which are disposed oppositely, are opposite in magnetism.
Further, the diameter of the centrifugal tube mounting hole is 11mm to 13 mm.
Further, the diameter of magnetic sheet mounting hole is 11mm to 13mm, and the degree of depth is 3mm to 4mm, and bearing structure's height is 3.5mm to 4.5mm, and thickness is 2mm to 3mm, and the distance of magnetic sheet mounting hole and base is 3mm to 4mm, and the thickness of magnetic sheet is 4mm to 5 mm.
Another aspect of the embodiments of the present invention is to provide a magnetic rack assembly, which includes any one of the magnetic racks, and further includes one or more centrifuge tubes.
The embodiment of the utility model provides a magnetic frame and magnetic frame subassembly, to sum up, the embodiment of the utility model provides a disclosed magnetic frame and magnetic frame subassembly are with the help of exposed magnetic sheet and the bearing structure who supports the magnetic sheet for the magnetic sheet can adjust its inclination in order to laminate with the centrifuging tube when the centrifuging tube inserts in the magnetic frame, has realized the in close contact with of magnetic sheet with the centrifuging tube, has strengthened the magnetic field intensity in the centrifugal tube area territory, saves the magnetic suction time.
Drawings
FIG. 1 is a cross-sectional schematic view of a magnetic mount according to an embodiment of the present disclosure;
figure 2 is a schematic cross-sectional view of a centrifuge tube inserted into the magnetic rack of figure 1.
The labels in the figure are: 1. a centrifugal tube plate; 2. a pillar; 3. a base; 4. a magnetic sheet mounting hole; 5. a support structure; 6. a magnetic sheet; 7. a centrifuge tube mounting hole; 8. and (5) fixing holes for centrifuge tubes.
Detailed Description
The magnetic frame and magnetic frame assembly of the present disclosure are described in further detail below with reference to the figures and the detailed description. Advantages and features of the present invention will become apparent from the following detailed description and claims. It is noted that the drawings are in greatly simplified form and employ non-precise ratios for the purposes of facilitating and distinctly facilitating the description of the embodiments of the present invention.
Referring to fig. 1, some embodiments of the present disclosure disclose a magnetic rack for magnetic bead purification. Specifically, the magnetic rack according to the embodiment of the present disclosure includes a centrifuge tube plate 1 and a base 3 located below the centrifuge tube plate 1, and a strut 2 connecting the centrifuge tube plate 1 and the base 3. A plurality of centrifuge tube mounting holes 7 are provided in the centrifuge tube plate 1 to accommodate the centrifuge tubes.
In some embodiments of the present disclosure, the centrifuge tube sheet 1 is provided with a plurality of centrifuge tube mounting holes 7 symmetrically about the central longitudinal axis of the magnetic rack, where longitudinal refers to the direction perpendicular to the cross-section shown in fig. 1. Preferably, the centrifuge tube plate 1 is uniformly provided with 24 centrifuge tube mounting holes 7, and 12 centrifuge tube mounting holes are respectively arranged on both sides of the support column 2. Due to the differences in diameter between 2ml and 1.5ml centrifuge tubes made by different manufacturers, for example, common centrifuge tubes have diameters between about 10mm and 11mm, with diameters no larger than 1 mm. To accommodate 2ml and 1.5ml centrifuge tubes from different manufacturers, the diameter of the centrifuge tube mounting holes 7 is configured to be slightly larger than the diameter of the 2ml or 1.5ml centrifuge tube, for example between 11mm and 13mm, or preferably between 11mm and 12.5mm, or preferably between 11mm and 12mm, or preferably between 11mm and 11.5mm, or preferably 11 mm. In this way, the centrifugal tube can be easily inserted into and removed from the centrifugal tube mounting hole 7 without applying a large force, and the centrifugal tube can be formed at a certain inclination angle after being inserted into the centrifugal tube mounting hole 7.
In some embodiments of the present disclosure, a plurality of sets of magnetic sheet mounting holes 4 are disposed on both sides of the pillar 2, and each set of magnetic sheet mounting holes 4 includes two magnetic sheet mounting holes disposed correspondingly. The magnetic sheet mounting hole 4 may be a circular hole or the like. In one embodiment, the magnet sheet mounting hole 4 has a diameter of about 11 to 13mm, preferably 11mm, and a hole depth of 3mm to 4 mm. Preferably, the distance from the magnetic sheet mounting hole 4 to the base 3 is 3mm to 4 mm. The magnetic sheet mounting holes 4 are preferably symmetrically arranged about the central longitudinal axis of the magnetic rack and correspond in position and number to the centrifuge tube mounting holes 7 in the centrifuge tube sheet 1 so that after the centrifuge tubes are mounted, each centrifuge tube is located substantially directly in front of the magnetic sheet mounting holes 4. That is, the correspondence of the positions of the magnetic sheet mounting hole 4 and the centrifugal tube mounting hole 7 is such that the projection of the magnetic sheet 6 on the first cross section overlaps the projection of the centrifugal tube mounting hole 7 on the first cross section (the first cross section is a plane perpendicular to the column 2). The inside bearing structure 5 that is provided with of magnetic sheet mounting hole 4, magnetic sheet 6 set up in magnetic sheet mounting hole 4 to support and lean on bearing structure 5. The support structure 5 is provided on the bottom surface or the side wall of the magnet sheet mounting hole 4. The support structure 5 is used to support the magnetic sheet 6. The two magnetic sheets 6 respectively positioned in the correspondingly arranged magnetic sheet mounting holes 4 are a group of magnetic sheets, and the magnetic poles of the two magnetic sheets 6 in the group of magnetic sheets which are oppositely arranged are opposite in magnetism. That is, when the magnetic pieces 6 are respectively received in a set of the correspondingly provided magnetic piece mounting holes 4, the magnetic pieces 6 are arranged such that the S poles and the N poles of the magnetic pieces 6 respectively positioned at both sides of the pole support 2 face each other (as shown in fig. 1), whereby the magnetic pieces 6 are held in the corresponding magnetic piece mounting holes 4 due to the magnetic attractive force therebetween. The magnetic sheet 6 is partially located outside the magnetic sheet mounting hole 4, that is, the magnetic sheet 6 is partially located outside an inner space defined by the bottom surface and the side wall of the magnetic sheet mounting hole 4. Thereby, the centrifuge tube can be in contact with at least the surface of the magnetic sheet 6 after installation. The magnetic sheet 6 is rotatable relative to the support structure 5, i.e. its inclination relative to the horizontal plane can be adjusted. The magnetic sheet 6 may be rotated about the support structure 5 by an external force applied to the magnetic sheet 6, such as by a user and/or a centrifuge tube. It should be noted that the magnetic sheet 6 may have a translational movement with respect to the support structure 5 during the rotation around the support structure 5. It should be understood at the same time that the magnetic frame is used to secure a centrifuge tube, and the magnetic sheet rotates around the support structure under the action of the centrifuge tube, magnetic sheet mounting holes, and other magnetic sheets.
In some embodiments of the present disclosure, the support structure 5 may be, for example, a boss or a cantilever, as shown in fig. 1. It will be appreciated that the boss cross-section may be rectangular, arcuate or polygonal in shape, or other suitable shape, or be of a rigid or elastomeric material.
In some embodiments of the present disclosure, the support structure 5 is disposed close to the lower portion of the magnetic sheet mounting hole 4, so that when the magnetic sheet 6 abuts against the support structure 5, the magnetic sheet 6 is inclined toward the pole support 2 due to the space existing in the upper portion of the magnetic sheet mounting hole 5, respectively, under the effect of magnetic attraction. That is, the support structure 5 is disposed close to the lower portion, i.e., the lower portion of the inner space defined by the bottom surface and the side wall of the magnet sheet mounting hole 4, while leaving a sufficient space in the upper portion of the inner space to accommodate a portion of the magnet sheet 6 so that the magnet sheet 6 can be obliquely abutted against the support structure 5. The height of the support structure 5 may be set not more than half of the radius or height of the magnet sheet mounting hole 5, and in the case where the diameter of the magnet sheet mounting hole 5 is about 11mm to 13mm, the diameter of the magnet sheet 6 may be about 10mm to 12mm, and the height of the support structure 5 is, for example, between 3.5mm to 4.5 mm. This configuration of the support structure 5 facilitates the contact of the magnetic sheet 6 with the wall of the centrifuge tube during the insertion of the centrifuge tube and the rotation or adjustment of the inclination under the effect of its pressing force.
For example, in the embodiment shown in fig. 1, the support structure 5 is a boss structure having a rectangular cross section and is located at the lower portion of the magnetic sheet mounting hole 4. Due to the bosses and the spaces above the bosses, under the action of magnetic attraction between the magnetic sheets 6, the magnetic sheets 6 are obliquely abutted against the bosses, particularly the fulcrums of the bosses to form inclined planes, so that the centrifuge tube can be conveniently inserted from top to bottom, and after the centrifuge tube is inserted, the magnetic sheets 6 rotate or the inclination angle is adjusted under the action of extrusion force of the centrifuge tube. The thickness of magnetic sheet 6 is such that when the centrifuge tube is placed in the magnetic rack through centrifuge tube mounting hole 7, the surface of magnetic sheet 6 can contact the sidewall of the centrifuge tube. For example, the height of the centrifuge tube is generally about 40mm, the height of the pillar of the magnetic rack is 20mm to 30mm, the distance between the centrifuge tube mounting hole 7 and the pillar 2 is 2mm to 3mm, the depth of the magnetic sheet mounting hole 4 relative to the pillar 2 is 3mm to 4mm, the distance (i.e., thickness) between the fulcrum of the support structure 5 and the bottom surface of the magnetic sheet mounting hole 4 is 2mm to 3mm, the height of the support structure 5 is 3.5mm to 4.5mm, and the thickness of the magnetic sheet 6 may be 4mm to 5mm in the case where the distance between the magnetic sheet mounting hole 4 and the base 3 is 3mm to 4 mm.
As shown in fig. 2, when inserting 2ml centrifuging tube, because the hypomere of 2ml centrifuging tube is cylindrical, 6 surfaces of magnetic sheet rotate around the fulcrum of boss under the extrusion force effect of centrifuging tube, and the inclination reduces, and magnetic sheet 6 is basically vertical placing, and the surface of this moment magnetic sheet 6 is laminated with the pipe wall of centrifuging tube (indicates basically laminating or at least partially laminating), is favorable to strengthening the magnetic field intensity in the centrifuging tube. When inserting 1.5ml centrifuging tube, as shown in fig. 2, the hypomere of centrifuging tube is the toper, and the surface of magnetic sheet 6 rotates around the summit of boss under the extrusion force effect of centrifuging tube, and the inclination is adjusted to the tapering phase-match with the centrifuging tube for the surface of magnetic sheet 6 is laminated basically with the pipe wall of centrifuging tube equally, has also strengthened the magnetic field intensity in the centrifuging tube. It will be appreciated that the advantageous features described above can also be achieved when the support structure 5 is a cantilever or other suitable structure.
In some embodiments of the present disclosure, the magnetic sheet 6 is made of one or more of neodymium iron boron, alnico, samarium cobalt. The magnetic sheet 6 may be cylindrical or rectangular or other shapes.
In some embodiments of the present disclosure, the base 3 is provided with a plurality of centrifuge tube securing holes 8 corresponding to the centrifuge tube mounting holes 7. The centrifuge tube fixing hole 8 can be a blind hole or a through hole. The tube securing hole 8 may be circular, oblong, oval, rectangular or other suitable shape. For example, the center of the centrifuge tube fixing hole 8 and the center of the centrifuge tube mounting hole 7 substantially coincide in a view perpendicular to the vertical direction, so that the centrifuge tube can be placed substantially vertically after being inserted into the magnetic rack, for example, the centrifuge tube central axis may have an angle of not more than 10 degrees, preferably not more than 5 degrees, more preferably not more than 3 degrees with the vertical direction. There are slight differences in the diameters of centrifugal tubes of different brands and models on the market, for example, the diameter difference is in the range of 1 mm. The centrifuge tube mounting holes 7 are sized so that the bottom of the centrifuge tube can move slightly within the centrifuge tube mounting holes 8 so that centrifuge tubes of different diameters can substantially conform to the surface of the magnetic sheet 6 by adjusting their inclination angles. For example, in the embodiment shown in figure 2, the centrifuge tube holding holes 8 are 4mm to 6mm in diameter, and a 2ml centrifuge tube is shown not to be placed perfectly vertically, but rather slightly tilted. By virtue of the magnetic sheet 6 being able to adjust its inclination, and the advantageous aspect that the centrifuge tube fixing holes 8 can move the centrifuge tube slightly therein, the magnetic rack according to some embodiments of the present disclosure can adapt to centrifuge tubes of slightly different diameters. In the embodiment shown in fig. 2, the magnetic frame can automatically adjust the inclination angle of the magnetic sheet 6 according to the diameter of the lower section of the 2ml and/or 1.5ml centrifuge tube to basically fit the centrifuge tube wall. Centrifuging tube and 6 contacts of magnetic sheet to receive its thrust and 8 holding power to the centrifuging tube bottom of centrifuging tube fixed orifices, the centrifuging tube realizes fixedly under the effect of these two power, has prevented the not hard up displacement that takes place at magnetic frame removal etc. in-process.
In embodiments of the present disclosure, a magnetic rack assembly is also disclosed, comprising any of the magnetic racks described above, further comprising one or more centrifuge tubes, such as one or more 2ml centrifuge tubes and/or one or more 1.5ml centrifuge tubes.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
Furthermore, the technical features mentioned in the different embodiments of the invention described below can be combined with each other as long as they do not conflict with each other.
As used in this disclosure and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It should also be understood that the term "and/or" as used herein refers to and encompasses any and all possible combinations of one or more of the associated listed items.
The terms "plurality" and "a plurality" in the present disclosure and appended claims refer to two or more than two unless otherwise specified.
It will be apparent to those skilled in the art that various modifications and variations can be made in the magnetic stand disclosed herein without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (10)
1. A magnetic rack comprises a centrifugal tube plate, a base positioned below the centrifugal tube plate, and a support connected with the centrifugal tube plate and the base, wherein the centrifugal tube plate is provided with a plurality of centrifugal tube mounting holes,
the base be provided with a plurality of with corresponding centrifuging tube fixed orifices of centrifuging tube mounting hole, the both sides of pillar are provided with multiunit magnetic sheet mounting hole, and each magnetic sheet mounting hole of group includes the magnetic sheet mounting hole that two set up correspondingly, be provided with bearing structure in the magnetic sheet mounting hole, the magnetic sheet sets up just support in the magnetic sheet mounting hole and lean on in bearing structure, the magnetic sheet is located partially the outside of magnetic sheet mounting hole for the centrifuging tube is inserting when in the magnetic frame, the surface of magnetic sheet with the centrifuging tube laminating.
2. The magnetic rack according to claim 1, wherein the support structure is a boss or a cantilever provided on a bottom surface and/or a side wall of the magnetic sheet mounting hole.
3. The magnetic rack of claim 2, wherein the support structure is adjacent to a lower portion of the magnet sheet mounting hole with respect to an upper portion of the magnet sheet mounting hole.
4. Magnetic stand according to claim 1, characterized in that the magnetic sheet is rotatable relative to the support structure.
5. A magnetic frame as claimed in claim 4, wherein the magnetic sheet is rotatable about the support structure under the action of an external force applied to the magnetic sheet.
6. The magnetic rack of claim 1, wherein the plane perpendicular to the support posts is a first cross section, and the projection of the magnetic sheet on the first cross section is partially overlapped with the projection of the centrifugal tube mounting hole on the first cross section.
7. The magnetic rack according to claim 1, wherein the two magnetic sheets respectively located in the corresponding magnetic sheet mounting holes are a group of magnetic sheets, and the magnetic poles of the two magnetic sheets in the group of magnetic sheets are opposite in polarity.
8. The magnetic rack of claim 1, wherein the centrifuge tube mounting holes are 11mm to 13mm in diameter.
9. The magnetic rack according to claim 1, wherein the magnetic sheet mounting hole has a diameter of 11mm to 13mm and a depth of 3mm to 4mm, the support structure has a height of 3.5mm to 4.5mm and a thickness of 2mm to 3mm, the magnetic sheet mounting hole is spaced from the base by 3mm to 4mm, and the magnetic sheet has a thickness of 4mm to 5 mm.
10. A magnet rack assembly comprising a magnet rack according to any of claims 1 to 9, further comprising one or more centrifuge tubes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020224246.8U CN211896992U (en) | 2020-02-27 | 2020-02-27 | Magnetic force frame and magnetic force frame subassembly |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020224246.8U CN211896992U (en) | 2020-02-27 | 2020-02-27 | Magnetic force frame and magnetic force frame subassembly |
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| CN211896992U true CN211896992U (en) | 2020-11-10 |
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| CN202020224246.8U Active CN211896992U (en) | 2020-02-27 | 2020-02-27 | Magnetic force frame and magnetic force frame subassembly |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI830144B (en) * | 2021-10-19 | 2024-01-21 | 英華達股份有限公司 | Selection device and magnetic rack |
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2020
- 2020-02-27 CN CN202020224246.8U patent/CN211896992U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI830144B (en) * | 2021-10-19 | 2024-01-21 | 英華達股份有限公司 | Selection device and magnetic rack |
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Address after: 510530 6th floor, building A2, No. 11, Kaiyuan Avenue, Science City, Guangzhou high tech Industrial Development Zone, Guangzhou City, Guangdong Province Patentee after: Guangzhou kangliming Biotechnology Co.,Ltd. Address before: 510530 6th floor, building A2, No. 11, Kaiyuan Avenue, Science City, Guangzhou high tech Industrial Development Zone, Guangzhou City, Guangdong Province Patentee before: Guangzhou Kliming Biotechnology Co.,Ltd. |
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