CN218811638U - Carriage assembly and nucleic acid extractor using same - Google Patents

Abstract

The utility model relates to a carrier assembly and use nucleic acid extraction appearance of this carrier assembly, include: the carrier plate is provided with at least one row of pipe frame grooves and a positioning part; each row of pipe frame grooves are formed with at least one through hole; the magnetic rod sleeve comprises a pipe frame, a fixing part and at least one pipe sleeve with one open end, and the pipe sleeve is fixed on the pipe frame; the positioning part is arranged on one side, facing the magnetic rod sleeve, of the pipe frame groove; the magnetic rod can penetrate through the through hole and is inserted into the corresponding pipe sleeve to achieve first positioning of the pipe sleeve, and the positioning portion is matched with the fixing portion to achieve second positioning of the pipe sleeve. The carrier assembly and the nucleic acid extractor using the carrier assembly have the advantage of accurate positioning.

Description

Carriage assembly and nucleic acid extractor using same

Technical Field

The application relates to the technical field of biological detection, in particular to a carrier assembly and a nucleic acid extractor using the carrier assembly.

Background

A nucleic acid extractor is an instrument which automatically finishes the extraction work of sample nucleic acid by using a matched nucleic acid extraction reagent, and the nucleic acid extractor is divided into two types: one type is largely automated and is commonly referred to as an automated liquid workstation; the other is a small automatic nucleic acid extractor which automatically finishes extraction by using a packaged matched reagent. The nucleic acid extraction method mainly adopts a magnetic bead method, and the principle of the magnetic bead method is as follows: the surface of the superparamagnetic nano-particles is improved and modified by using a nanotechnology to prepare superparamagnetic silicon oxide nano-magnetic beads. The magnetic beads can be specifically identified and efficiently combined with nucleic acid molecules on a micro interface. By utilizing the superparamagnetism of the silicon oxide nano microspheres, DNA and RNA are separated from samples such as blood, animal tissues, food, pathogenic microorganisms and the like under the action of Chaotropic salts (guanidine hydrochloride, guanidine isothiocyanate and the like) and an external magnetic field.

When the nucleic acid extractor extracts nucleic acid by the principle of a magnetic bead method, a magnetic rod sleeve is sleeved outside the magnetic rod, and DNA and RNA adsorbed by the magnetic rod are attached to the outside of the magnetic rod sleeve. However, the magnetic rod sleeve and the magnetic rod are easy to be positioned in a deviation manner when being sleeved, so that the magnetic attraction effect on DNA and RNA is influenced.

SUMMERY OF THE UTILITY MODEL

In view of the above, it is desirable to provide a carrier assembly and a nucleic acid extractor using the same, which can solve the problem that the positioning of the magnetic rod inside the sleeve of the magnetic rod sleeve is easily deviated when the magnetic rod sleeve is sleeved with the magnetic rod.

A carrier assembly for a nucleic acid extractor having at least one magnetic rod, the carrier assembly comprising: the carrier plate is provided with at least one row of pipe frame grooves and a positioning part; each column of pipe frame grooves are formed with at least one through hole; the magnetic rod sleeve comprises a pipe frame, a fixing part and at least one pipe sleeve with an opening at one end, the pipe sleeve is fixed on the pipe frame, and the fixing part is arranged on the pipe frame or the pipe sleeve; the positioning part is arranged on one side, facing the magnetic rod sleeve, of the pipe frame groove; the bar magnet can pass the through-hole and insert the correspondence in the pipe box, in order to realize the first location of pipe box, the pipe support embedding in the pipe support groove, location portion with fixed part cooperation location is in order to realize the second location of pipe box.

In one embodiment, the positioning part is a bump, the positioning part is arranged on the bottom surface of the pipe frame groove facing the magnetic rod sleeve, and one through hole penetrates through the positioning part;

one end of the opening of the pipe sleeve close to the through hole is formed as the fixing part.

In one embodiment, the positioning part is arranged on the bottom surface of the pipe frame groove facing the magnetic rod sleeve, and the fixing part is arranged on the pipe frame;

one of the positioning part and the fixing part is a positioning column, and the other is a positioning hole.

In one embodiment, the positioning part is a conical column; the fixing part is a tapered hole matched with the positioning part.

In one embodiment, each row of the pipe frame grooves is provided with at least one positioning part, and one magnetic rod sleeve comprises at least one fixing part matched with the positioning part.

In one embodiment, the positioning part is detachably arranged on the bottom surface of the slot of the carrier plate facing the magnetic rod sleeve.

In one embodiment, the carrier assembly comprises a positioning clamping bead arranged on the carrier plate, and when the positioning part is matched and positioned with the fixing part, the positioning clamping bead is clamped on the side wall of the pipe frame.

In one embodiment, the two side groove walls of the pipe frame groove are provided with mounting holes, and the positioning clamp balls are arranged in the mounting holes;

step parts are arranged on two sides of the pipe frame;

when the positioning part is matched and positioned with the fixing part, the positioning clamping bead is propped against the oblique lower part of the step part.

In one embodiment, the carrier plate has a connecting portion formed at an end thereof, and the connecting portion has a connecting hole formed therethrough.

A nucleic acid extractor, comprising: the carrier assembly described above; a magnetic bar; the first driving mechanism is connected with the carrier plate; the second driving mechanism is connected with the magnetic rod; and a deep hole plate having a plurality of deep holes; the pipe sleeves are inserted into the deep holes, the magnetic rods can downwards penetrate through the through holes and are inserted into the corresponding pipe sleeves under the driving of the second driving mechanism, and the carrier plate can downwards move under the driving of the first driving mechanism until the positioning portion is matched with the fixing portion for positioning.

In one embodiment, the carrier plate can move upwards under the driving of the first driving mechanism, and the magnetic rod can move downwards under the driving of the second driving mechanism until the bottom of the magnetic rod is abutted against the bottom of the pipe sleeve so as to overcome the limit of the positioning clamping bead and separate the carrier plate and the magnetic rod sleeve.

The beneficial effects are that: the carrier assembly of the embodiment of the application is characterized in that the carrier plate and at least one magnetic rod sleeve are arranged; the carrier plate is provided with at least one row of pipe frame grooves and a positioning part; at least one through hole is formed in each tube rack groove. The magnetic rod sleeve comprises a pipe frame, a fixing part and at least one pipe sleeve with an opening at one end, and the pipe sleeve is fixed on the pipe frame; the positioning part is arranged on one side of the pipe frame groove facing the magnetic rod sleeve; the magnetic rods can pass through the through holes and are inserted into the corresponding pipe sleeves to realize the first positioning of the pipe sleeves, so that the pipe frame is basically aligned with the pipe frame groove; through location portion and fixed part cooperation location to make pipe support and pipe support groove location, and then can fix a position the pipe box of fixing relatively with the pipe support, avoid the pipe box to produce positioning error.

Drawings

FIG. 1 is a three-dimensional block diagram of a carrier assembly according to an embodiment of the present application;

fig. 2 is a three-dimensional structural view of a carrier plate according to an embodiment of the present application;

FIG. 3 is a three-dimensional block diagram of a magnet sleeve according to an embodiment of the present application;

FIG. 4 is a schematic view of the carriage assembly of FIG. 1 from another perspective;

FIG. 5 isbase:Sub>A cross-sectional view A-A of the carriage assembly of FIG. 4;

fig. 6 is a structural view of a carrier plate according to another embodiment of the present application;

fig. 7 is a schematic view of a portion of a carrier plate according to yet another embodiment of the present application;

FIG. 8 is a structural view of a magnet bar sleeve according to another embodiment of the present application;

FIG. 9 is a three-dimensional structural view of a nucleic acid extracting apparatus according to an embodiment of the present application, in which a magnetic rod and a carriage assembly are separated;

FIG. 10 is a three-dimensional structural view of the nucleic acid extracting apparatus according to the embodiment of the present application, in which a magnetic rod is inserted into a through hole;

FIG. 11 is a three-dimensional structural view of the nucleic acid isolation instrument according to the embodiment of the present application, in which a magnetic rod is inserted into a tube housing.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present application more comprehensible, embodiments accompanying the present application are described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application. This application is capable of embodiments in many different forms than those described herein and that modifications may be made by one skilled in the art without departing from the spirit and scope of the application and it is therefore not intended to be limited to the specific embodiments disclosed below.

In the description of the present application, it is to be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the present application and for simplicity in description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be considered limiting of the present application.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present application, "plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In this application, unless expressly stated or limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can include, for example, fixed connections, removable connections, or integral parts; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.

In this application, unless expressly stated or limited otherwise, a first feature is "on" or "under" a second feature such that the first and second features are in direct contact, or the first and second features are in indirect contact via an intermediary. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.

Referring to fig. 1 to 11, a carrier assembly 100 according to a first embodiment of the present application, the carrier assembly 100 being used in a nucleic acid extractor having at least one magnetic rod 200; generally, the number of magnetic rods 200 in the nucleic acid isolation apparatus is maintained at 16 or more, and the magnetic rods 200 are arranged in a row for every 8 magnetic rods.

The carrier assembly 100 includes: carrier plate 10 and at least one magnet bar sleeve 20. The carrier plate 10 is provided with at least one row of pipe frame grooves 11 and a positioning part 12; at least one through hole 111 is formed in each row of pipe rack grooves 11; the through holes 111 correspond to the magnetic rods 200, and when 8 magnetic rods 200 are arranged in a row, 8 through holes 111 arranged along the first direction are correspondingly formed on a row of the pipe frame grooves 11. Each magnetic rod sleeve 20 comprises a pipe frame 21, a fixing part 22 and at least one pipe sleeve 23 with one open end, the pipe sleeve 23 is fixed on the pipe frame 21, similarly, the pipe sleeve 23 corresponds to the magnetic rod 200, when 8 magnetic rods 200 are arranged in a row, 8 pipe sleeves 23 arranged along the first direction are correspondingly fixed on one pipe frame 21. The fixing portion 22 may be provided on the pipe frame 21 or the pipe sleeve 23.

In the embodiments of the present application, the first direction may be defined as an extending direction of the pipe frame groove 11.

The pipe sleeves 23 are generally in one-to-one correspondence with the through holes 111. In the process of extracting nucleic acid, the pipe sleeve 23 is inserted into the deep hole 510 of the deep hole plate 500 in advance, and during the movement of the magnetic rod 200, the head of the magnetic rod 200 firstly passes downward through the through hole 111 of the carrier plate 10 and then is inserted into the pipe sleeve 23.

In the related art, because of there is liquid in the deep hole of deep hole board, place inside when the pipe box of bar magnet cover, can produce corresponding buoyancy, though the effect of buoyancy is whole upwards, the existence of buoyancy can lead to the pipe box to have the slope of randomness, and then leads to the bar magnet that inserts in the pipe box to appear positioning deviation. In addition, the deep hole and the pipe sleeve are both long pipe fittings, the positioning deviation can be further amplified by a longer length, so that the pipe sleeve of the magnetic rod sleeve is obliquely inserted into the deep hole, the space on one side, close to the pipe sleeve, of the interior of the deep hole is smaller and is far away from the magnetic rod, the magnetic attraction effect is weak, and the magnetic beads with adsorbed nucleic acid are attracted to the side of the pipe sleeve under weaker magnetic force; the space of one side of the interior of the deep hole, which is far away from the pipe sleeve, is larger, the side is close to the magnetic rod, the magnetic attraction effect is strong, and the magnetic beads which adsorb nucleic acid are adsorbed on the side of the pipe sleeve under stronger magnetic force; for the pipe sleeve, the speed of adsorbing magnetic beads with nucleic acid on two sides is different, so that the magnetic attraction effect on the outer side of the magnetic rod sleeve is influenced, and further the nucleic acid extraction effect and the detection precision are influenced.

In the embodiment of the present application, the positioning portion 12 is disposed on one side of the pipe frame groove 11 facing the magnetic rod sleeve 20; the magnetic rod 200 can pass through the through hole 111 and be inserted into the corresponding socket 23 to achieve the first positioning of the socket 23; namely, the pipe housing 23 inserted into the deep hole 510 of the deep hole plate 500 (mentioned later) is first positioned by the magnet bar 200 so that the pipe housing 21 is substantially aligned with the pipe housing groove 11.

In the nucleic acid extractor, the direction of the magnetic rod 200, the direction of the through-hole 111 in the carrier plate 10, and the direction of the deep hole 510 in the deep hole plate 500 are generally along the second direction. While the pipe sleeve 23 inserted into the deep hole 510 has a gap therebetween, the diameter of the magnetic rod 200 is also generally smaller than that of the pipe sleeve 23, so that the pipe sleeve 23 is virtually floated during the nucleic acid extraction process.

In the embodiment of the application, when the pipe frame 21 is embedded into the pipe frame groove 11, the positioning part 12 is matched with the fixing part 22 for positioning so as to realize the second positioning of the pipe sleeve 23; namely, the positioning part 12 and the fixing part 22 are used for carrying out the second positioning, so that the pipe frame 21 and the pipe frame groove 11 are positioned, and the pipe sleeve 23 fixed relative to the pipe frame 21 can be positioned and does not float left and right; the pipe sleeve 23 is prevented from generating positioning errors; therefore, the pipe sleeve 23 is kept vertical in the deep hole 510, the influence of buoyancy on the pipe sleeve 23 is reduced, the pipe sleeve 23 and the magnetic rod 200 are coaxial and cannot incline, and the space from the inner periphery of the deep hole 510 to the pipe sleeve 23 can be kept uniform.

The second positioning of the positioning part 12 and the fixing part 22 can be a clamping positioning or an embedding positioning.

In some embodiments, as shown in FIGS. 1-6; the positioning part 12 is a projection, the positioning part 12 is disposed on the bottom surface 11a of the tube frame groove 11 facing the magnet bar cover 20, and one through hole 111 penetrates the positioning part 12. An end of the opening of the socket 23 near the through hole 111 is formed as a fixing portion 22.

The tube housings 23 have open ends 231, and the open ends 231 of the plurality of tube housings 23 are fixed to the tube frame 21.

The fixing portion 22 is adapted to the positioning portion 12, that is, the fixing portion 22 has the same shape and size as or slightly larger than the positioning portion 12; when the magnetic rod sleeve 20 is matched with the carrier plate 10, the positioning part 12 can be embedded into the fixing part 22 to form a second positioning, so that the pipe sleeve 23 is prevented from deviating, a positioning error between the pipe sleeve 23 and the magnetic rod 200 is avoided, and the detection precision is improved.

In the embodiment of the present application, the open end 231 of the sleeve 23 is the fixing portion 22; the positioning portion 12 may be a tapered column; wherein, one end of the positioning part 12 facing the magnetic rod sleeve 20 is a small diameter end, and one end of the positioning part 12 close to the carrier plate 10 is a large diameter end; the fixing portion 22 may be a tapered hole adapted to the positioning portion 12. The fixing portion 22 has a flared cone shape with a larger upper portion and a smaller lower portion along the second direction, so that the fixing portion 22 and the positioning portion 12 can be conveniently positioned in a guiding fit manner.

In some embodiments, referring to fig. 7 and 8, the positioning portion 12 is disposed on the bottom surface 11a of the pipe frame groove 11 facing the magnet bar cover 20, and the fixing portion 22 is disposed on the pipe frame 21. The open end 231 of the socket 23 and the fixing portion 22 have two different structures. By designing the positions of the fixing portion 22 and the positioning portion 12, when the magnetic rod sleeve 20 is matched with the carrier plate 10, the fixing portion 22 can be embedded into the positioning portion 12 to form a second positioning, so that the pipe sleeve 23 is prevented from deviating, a positioning error between the pipe sleeve 23 and the magnetic rod 200 is avoided, and the detection precision is improved.

One of the positioning portion 12 and the fixing portion 22 is a positioning post, and the other is a positioning hole; that is, when the positioning portion 12 is a positioning post and the fixing portion 22 is a corresponding positioning hole; when the positioning portion 12 is a positioning hole, the fixing portion 22 is a corresponding positioning post. Through embedding the positioning column into the positioning hole, the second positioning of the positioning part 12 and the fixing part 22 is realized, the positioning error is avoided, and the detection precision is finally improved.

Similarly, in the embodiment of the present application, if the positioning portion 12 is a positioning column and the fixing portion 22 is a positioning hole, the positioning portion 12 can also be designed in a shape of a tapered column; the fixing portion 22 has a tapered hole shape fitted to the positioning portion 12.

In some embodiments, referring to fig. 1 to 8, each row of the bobbin grooves 11 has at least one positioning portion 12, and one of the magnetic rod sleeves 20 includes at least one fixing portion 22 matching with the positioning portion 12. Thereby performing the second positioning by the positioning portion 12 and the corresponding fixing portion 22. Two positioning parts 12 arranged along a first direction can be arranged on the pipe frame groove 11, a straight line is formed between the two parts, the positioning precision is ensured, the pipe frame 21 and the pipe frame groove 11 are prevented from shaking during positioning, and the pipe sleeve 23 is prevented from generating positioning errors; the detection precision is improved.

In some embodiments, referring to fig. 1 to 5, each row of rack grooves 11 may be provided with 8 through holes 111, and correspondingly, 8 sleeves are provided on one magnetic rod sleeve 20, wherein the 2 nd and 2 nd to last through holes 111 are provided with positioning parts 12 along the first direction. Can set up bilateral symmetry's two rows pipe support grooves 11 on the carrier plate 10, a carrier plate 10 can carry out nucleic acid extraction work with two bar magnet cover 20 cooperations simultaneously, very big improvement nucleic acid extraction's efficiency.

In some embodiments, referring to fig. 1 to 5 and fig. 7, the positioning portion 12 is disposed on a side of the frame groove 11 facing the magnetic rod sleeve 20, and the two may be integrally formed.

In other embodiments, referring to fig. 6, the positioning portion 12 is separate from the pipe rack slot 11, the positioning portion 12 is detachably disposed on the slot bottom surface 11a of the rack plate 10 facing the magnetic rod sleeve 20, for example, the positioning portion 12 is fixed on the slot bottom surface 11a by screwing, clipping, etc., so that when the positioning portion 12 is worn, it is only necessary to detach the positioning portion 12 and mount a new positioning portion 12 on the slot bottom surface 11 a.

In some embodiments, referring to fig. 1 to 5 and fig. 7, the carriage assembly includes a positioning bead 30 disposed on the carriage plate 10, and when the positioning portion 12 is positioned by matching with the fixing portion 22, the positioning bead 30 is clamped on the sidewall of the pipe frame 21; the positioning clamping beads 30 have springs inside and retractable clamping beads at the ends thereof, and are supported on the side walls of both sides of the pipe frame 21 by the clamping beads to provide lateral supporting forces, respectively, so that the pipe frame 21 can be inserted into the pipe frame groove 11.

Specifically, mounting holes 112 are formed in the wall of each of the two side walls of the pipe frame groove 11, and the positioning clamping beads 30 are arranged in the mounting holes 112; step parts 211 are arranged on two sides of the pipe frame 21; through the overall length of the positioning clamping bead 30 which is reasonably designed, the positioning clamping bead 30 can just expose the clamping bead to the pipe frame groove 11. As shown in fig. 5, when the positioning portion 12 is matched and positioned with the fixing portion 22, the positioning clamping bead 30 abuts against the obliquely lower portion of the step portion 211, the side edge of the step portion 211 can abut against the obliquely upper portion of the positioning clamping bead 30, and the positioning clamping bead 30 applies a rightward and upward force to the positioning clamping bead 30, so that the pipe frame 21 can be tightly attached to the bottom surface 11a of the pipe frame groove 11 without being loosened, and the subsequent detection procedure can be ensured.

In some embodiments, as shown in fig. 1 to 6, the carrier plate 10 has an end portion formed with a connecting portion 13 perpendicular to the first direction, and the connecting portion 13 has a connecting hole 131 formed therethrough. The connecting portion 13 protrudes from the plate surface of the carrier plate 10 in the second direction, and a first driving mechanism 300 (mentioned later) is connected to the carrier plate 10 by a bolt passing through the connecting hole 131, thereby facilitating automation of the nucleic acid extracting work.

The second embodiment of the present application provides a nucleic acid extractor, which includes the above-mentioned carrier assembly 100, magnetic rod 200; a first driving mechanism 300, a second driving mechanism 400, and a deep hole plate 500. The first driving mechanism 300 may be an air cylinder or a motor acting along the second direction, and the first driving mechanism 300 is connected to the carrier plate 10 to move the carrier plate 10 up and down. The second driving mechanism 400 may be a cylinder or a motor acting in the second direction, and the second driving mechanism 400 is connected to the magnetic rod 200 to drive the magnetic rod 200 to move up and down. The deep hole plate 500 has a plurality of deep holes 510;

the pipe sleeves 23 are inserted into the deep holes 510, the magnetic rods 200 can downwards penetrate through the through holes 111 and be inserted into the corresponding pipe sleeves 23 under the driving of the second driving mechanism 400, and the carrier plate 10 can downwards move until the positioning parts 12 are matched and positioned with the fixing parts 22 under the driving of the first driving mechanism 300; through the cooperation location of location portion 12 and fixed part 22, can avoid pipe box 23 to produce positioning error to ensure the magnetic effect of pipe box 23 periphery, improved the detection precision.

After the extraction of nucleic acid is completed, the carrier plate 10 can move upward under the drive of the first driving mechanism 300, and the carrier plate 10 drives the magnetic rod sleeve 20 to move upward together due to the restriction of the positioning card bead 30; meanwhile, the magnetic rod 200 remains stationary, or the magnetic rod 200 can move downward by the second driving mechanism 400; therefore, the bottom of the magnetic rod 200 collides with the bottom of the pipe sleeve 23, the limit of the positioning clamping bead 30 on the pipe frame 21 is overcome when the bottom of the pipe sleeve 23 is subjected to resistance, the pipe frame 21 is separated from the pipe frame groove 11, the magnetic rod sleeve 20 is separated from the carrier plate 10, preparation is made for subsequent nucleic acid extraction, and the workload of operators is reduced.

The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present application, and the description thereof is more specific and detailed, but not construed as limiting the claims. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the concept of the present application, which falls within the scope of protection of the present application. Therefore, the protection scope of the present patent application shall be subject to the appended claims.

Claims (11)

1. A carriage assembly for a nucleic acid extractor having at least one magnetic bar (200), the carriage assembly comprising:

the device comprises a carrier plate (10), wherein the carrier plate (10) is provided with at least one row of pipe frame grooves (11) and a positioning part (12); at least one through hole (111) is formed in each column of the pipe frame grooves (11);

the magnetic rod sleeve (20) comprises a pipe frame (21), a fixing part (22) and at least one pipe sleeve (23) with one open end, the pipe sleeve (23) is fixed on the pipe frame (21), and the fixing part (22) is arranged on the pipe frame (21) or the pipe sleeve (23);

the positioning part (12) is arranged on one side of the pipe frame groove (11) facing the magnetic rod sleeve (20);

the magnetic rod (200) can pass through the through hole (111) and be inserted into the corresponding pipe sleeve (23) to realize first positioning of the pipe sleeve (23), the pipe frame (21) is embedded into the pipe frame groove (11), and the positioning part (12) is matched and positioned with the fixing part (22) to realize second positioning of the pipe sleeve (23).

2. A carriage assembly as claimed in claim 1, characterised in that the detent (12) is a projection, the detent (12) being provided on a slot bottom surface of the tube holder slot (11) facing the magnet bar sleeve (20), wherein one of the through holes (111) penetrates the detent (12);

one end of the opening of the pipe sleeve (23) close to the through hole (111) is formed as the fixing portion (22).

3. The carrier assembly of claim 1, wherein the locating portion (12) is provided on a bottom surface of the tube support channel (11) facing the magnet bar sleeve (20), the fixing portion (22) being provided on the tube support (21);

one of the positioning part (12) and the fixing part (22) is a positioning column, and the other one is a positioning hole.

4. The carrier assembly of claim 2, wherein the locating portion (12) is a tapered post;

the fixing part (22) is a taper hole matched with the positioning part (12).

5. A carrier assembly as claimed in any one of claims 1 to 4, in which each row of the tube support slots (11) has at least one locating portion (12) thereon, and one of the magnet bar sleeves (20) includes at least one fixing portion (22) which is adapted to the locating portion (12).

6. A carrier assembly according to any one of claims 1 to 4, characterised in that the locating portion (12) is detachably provided on a slot bottom surface of the carrier plate (10) facing the magnet bar sleeve (20).

7. The carriage assembly of any of claims 1 to 4, characterized in that the carriage assembly comprises a detent bead (30) provided on the carriage plate (10), the detent bead (30) being snapped onto a side wall of the pipe frame (21) when the positioning portion (12) is positioned in cooperation with the fixing portion (22).

8. The carrier assembly as claimed in claim 7, wherein mounting holes (112) are formed in both side wall of the pipe frame groove (11), and the positioning clamp beads (30) are arranged in the mounting holes (112);

steps (211) are arranged on two sides of the pipe frame (21);

when the positioning part (12) is matched and positioned with the fixing part (22), the positioning clamping bead (30) is propped against the oblique lower part of the step part (211).

9. A carrier assembly as claimed in any of claims 1 to 4, wherein the carrier plate (10) is formed with a connecting portion (13) at an end thereof, the connecting portion (13) being formed with a connecting aperture (131) therethrough.

10. A nucleic acid extractor using the carriage assembly, the nucleic acid extractor comprising:

the carrier assembly (100) of any of claims 1 to 9;

a magnetic bar (200);

a first drive mechanism (300) connected to the carriage plate (10);

a second drive mechanism (400) connected to the magnetic bar (200);

and a deep hole plate (500) having a plurality of deep holes (510);

the pipe sleeve (23) is inserted into the deep hole (510), the magnetic rod (200) can downwards penetrate through the through hole (111) and be inserted into the corresponding pipe sleeve (23) under the driving of the second driving mechanism (400), and the carrier plate (10) can downwards move until the positioning part (12) is matched and positioned with the fixing part (22) under the driving of the first driving mechanism (300).

11. The nucleic acid extractor according to claim 10, wherein the carrier plate (10) is capable of moving upward by the first driving mechanism (300), and the magnetic rod (200) is capable of moving downward by the second driving mechanism (400) until the bottom of the magnetic rod (200) abuts against the bottom of the socket (23) to overcome the limit of the detent ball (30) and separate the carrier plate (10) and the magnetic rod socket (20).

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