CN218272095U

CN218272095U - A sealing washer auxiliary positioning device for nucleic acid analysis gel column capillary

Info

Publication number: CN218272095U
Application number: CN202222188999.XU
Authority: CN
Inventors: 何春燕
Original assignee: Qiagen Shenzhen Co Ltd
Current assignee: Qiagen Shenzhen Co Ltd
Priority date: 2022-08-19
Filing date: 2022-08-19
Publication date: 2023-01-10
Anticipated expiration: 2032-08-19

Abstract

A seal ring assisted positioning device for a nucleic acid analysis gel column capillary, comprising: a plurality of positioning components and a support frame; the supporting frame is provided with a plurality of accommodating grooves, and the side groove walls of the accommodating grooves are provided with notches communicated with the insides of the accommodating grooves; each positioning component corresponds to one accommodating groove respectively and is accommodated in the accommodating groove, each positioning component is provided with a positioning end exposed out of the support frame from the notch, and the positioning end extends from the end part to the inside of the positioning end to form an accommodating hole; the positioning end is used for abutting against the sealing ring in the sealing ring carrying part of the clamping clamp so as to position the sealing ring, and the accommodating hole is used for accommodating a capillary tube penetrating through the hole part of the sealing ring. This device can be fixed a position a plurality of sealing washers on the middle part support of nucleic acid analysis gel column simultaneously, can effectively improve work efficiency to reduce the cost of labor.

Description

A sealing washer auxiliary positioning device for nucleic acid analysis gel column capillary

Technical Field

The application relates to the technical field of capillary tube detection of nucleic acid analysis gel columns, in particular to a sealing ring auxiliary positioning device for a capillary tube of a nucleic acid analysis gel column.

Background

The nucleic acid analysis gel column (called as a clamp for short) is mainly used as a carrier for carrying out high-sensitivity and high-resolution macromolecular automatic analysis procedures such as DNA or RNA and the like on a sample in a nucleic acid analyzer, and is mainly applied to the aspects of genetics, disease screening, DNA fingerprint analysis, pathogenic microorganism monitoring analysis and the like. The production process of the clamp comprises the steps of incoming material cleaning, middle and bottom support assembly, capillary tube inspection, pressure cleaning and packaging after QC inspection is qualified. The capillary tube inspection process of the process flow needs to firstly penetrate the capillary tube into the clamp and adjust the capillary tube to be exposed for a certain length, and when the capillary tube is adjusted, the other end of the capillary tube needs to penetrate through the capillary tube sealing ring, and the sealing ring needs to be fixed at a corresponding position by adopting the capillary tube sealing ring positioning assembly so as to ensure that the sealing ring can be sleeved on the outer tube wall of the capillary tube and avoid water leakage and glue leakage caused by the looseness of the sealing ring. However, the traditional capillary sealing ring positioning component can only block one capillary sealing ring at each time, the capillary positioning component needs to be repeatedly moved for many times in the working process, and meanwhile, because the distance between capillaries is small, the capillaries of other channels are easily touched in the process of adjusting the capillaries, so that a large amount of repeated work can be caused, the labor cost is increased, and the working efficiency is also reduced.

SUMMERY OF THE UTILITY MODEL

The application aims at providing a sealing washer auxiliary positioning device for nucleic acid analysis gel column capillary can fix a position a plurality of sealing washers on the nucleic acid analysis gel column simultaneously, is convenient for wear to establish a plurality of capillary simultaneously in a plurality of sealing washers simultaneously to reduce the cost of labor, and improve work efficiency.

The application provides a sealing washer assistance-localization real-time device for nucleic acid analysis gel column capillary, includes: a plurality of positioning components and a supporting frame are supported; the supporting frame is provided with a plurality of accommodating grooves, and the end parts of the accommodating grooves are provided with notches communicated with the insides of the accommodating grooves; each positioning assembly corresponds to one accommodating groove respectively, the positioning assembly is accommodated in the accommodating groove, the positioning assembly is provided with a positioning end exposed out of the notch, and the positioning end extends from the end part to the inside of the positioning end to form an accommodating hole; the positioning end is used for abutting against the sealing ring in the sealing ring carrying part of the clamping clamp so as to position the sealing ring, and the accommodating hole is used for accommodating a capillary tube penetrating from the hole passing part of the sealing ring.

In one embodiment, the method further comprises: the clamping assembly is used for clamping and positioning the positioning assemblies in the accommodating grooves of the supporting frame.

In one embodiment, the support frame comprises: the partition plates are sequentially arranged on the bottom plate at intervals, and the accommodating groove is formed between every two adjacent partition plates; the clamping assembly is mounted to the base plate.

In one embodiment, the clamping assembly comprises: the two supporting pieces are respectively and rotatably arranged on two opposite sides of the bottom plate, and the plurality of partition plates are arranged between the two opposite sides of the bottom plate; the clamping piece is rotatably arranged between the two supporting pieces; the clamping piece is provided with two elastic arms, and the free ends of the two elastic arms are provided with clamping parts; the clamping part is used for clamping the clamping groove, so that the positioning assembly is positioned in the accommodating groove through the clamping piece.

In one embodiment, the retaining grooves extend along the length direction of the side edges of the two opposite sides of the bottom plate.

In one embodiment, the bottom plate is magnetically and fixedly connected with the partition plate.

In one embodiment, the bottom plate is the formula bottom plate is inhaled to magnetism by the preparation of magnet, and/or, the baffle is the formula baffle is inhaled to magnetism by the preparation of magnet.

In one embodiment, a buffer component for buffering the positioning component is further arranged at the bottom of the accommodating groove.

In one embodiment, the positioning end is a cylindrical positioning end with a cylindrical shape, the outer diameter of the cylindrical positioning end is smaller than or equal to the diameter of the sealing ring carrying part, and the aperture of the accommodating hole is smaller than that of the sealing ring through hole part.

According to the utility model provides a sealing washer auxiliary positioning device for nucleic acid analysis gel column capillary, set up the support frame in the top of the middle part support of clamping, and the accommodation hole that keeps the locating end is coaxial with the year thing hole of objective table, place the sealing washer in sealing washer year thing portion, remove the support frame and make locating component's locating end support the sealing washer of holding in sealing washer year thing portion, in order to support the sealing washer and hold in sealing washer year thing portion, the capillary is crossed the hole portion from the sealing washer to the opposite side that the middle part support of clamping deviates from in sealing washer year thing portion and is worn to establish through the sealing washer, wear to establish the in-process, because the support effect of locating end, guarantee that the sealing washer can not remove along with the removal of capillary, the capillary is in the in-process of crossing the hole portion from the sealing washer, can make the sealing washer cover establish on the outer pipe wall of capillary, the setting of accommodation hole can provide the removal space for the removal of capillary. Compared with the mode of only positioning one sealing ring at each time in the prior art, the device can simultaneously position a plurality of sealing rings on the middle support of the nucleic acid analysis gel column, can effectively improve the working efficiency and reduce the labor cost.

Drawings

FIG. 1 is a perspective view of a sealing ring-assisted positioning device for a capillary tube of a nucleic acid analysis gel column provided herein;

FIG. 2 is a cross-sectional view of a seal ring assisted positioning device for a capillary tube of a nucleic acid analysis gel column provided herein;

FIG. 3 is an enlarged view of a portion of FIG. 2 at A;

FIG. 4 is a schematic view of the seal ring loading portion not carrying the seal ring in the loading hole of the middle bracket of the clamp;

FIG. 5 is a perspective view of a support frame in the positioning device for assisting the sealing ring of the capillary tube of the gel column for nucleic acid analysis provided in the present application;

FIG. 6 is a cross-sectional view of a support frame in a sealing ring-assisted positioning device for a capillary tube of a nucleic acid analysis gel column according to the present application;

FIG. 7 is an enlarged partial view of FIG. 6 at B;

FIG. 8 is a schematic view of a spacer and a positioning assembly in a sealing ring assisted positioning device for a capillary tube of a nucleic acid analysis gel column according to the present application;

FIG. 9 is a schematic diagram of a positioning assembly and a partial clip of the positioning device for assisting positioning of a capillary tube of a nucleic acid analysis gel column according to the present invention.

The reference numbers illustrate:

the positioning component 10, the positioning end 11, the accommodating hole 111 and the conical section 12;

the support frame 20, the accommodating groove 21, the notch 211, the bottom plate 22, the clamping groove 221, the second clamping surface 222 and the partition plate 23;

the middle support 30 of the clamp, a loading hole 31, a sealing ring loading part 311, a sealing ring through hole part 312, a groove 32, a separation bulge 33 and a separation cavity 34;

the clamping assembly 40, the clamping member 41, the supporting member 42, the first rotating shaft 421, the second rotating shaft 411, the clamping part 412, the first clamping surface 413;

a cushioning assembly 50;

capillary 100, sealing washer 200.

Detailed Description

The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in this specification in order not to obscure the core of the present application with unnecessary detail, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

The application provides a sealing washer auxiliary positioning device for nucleic acid analysis gel column capillary, wherein, nucleic acid analysis gel column capillary is for being applied to capillary electrophoresis technique for genetic analysis, disease detection etc.. This sealing washer auxiliary positioning device can fix a position a plurality of sealing washers on the middle part support of nucleic acid analysis gel column simultaneously to make a plurality of capillaries wear to locate the sealing washer simultaneously, compare in the mode that can only fix a position a sealing washer at every turn in the past, can effectively improve work efficiency, and reduce the cost of labor. The capillary usually need wear to establish in the capillary passageway of middle part support, with the sealing washer in this device can make and reach sealed effect between the capillary passageway of capillary and middle part support, the phenomenon of leaking glue when avoiding the encapsulating. Through this sealing washer auxiliary positioning device.

Referring to fig. 1 to 5, the sealing ring auxiliary positioning device for a capillary tube of a nucleic acid analysis gel column provided in this embodiment includes: a plurality of positioning components 10 and a support frame 20.

As shown in fig. 1 and 5, the support bracket 20 is provided with a plurality of receiving grooves 21, and a notch 211 communicating with the inside of the receiving groove 21 is provided at an end of the receiving groove 21. Each positioning component 10 corresponds to one receiving groove 21, and the positioning component 10 is received in the receiving groove 21. In this embodiment, taking the view angles shown in fig. 1 and 5 as an example, the accommodating groove 21 is a strip-shaped accommodating groove, and a notch 211 is disposed at the bottom end of the accommodating groove 21. The positioning assembly 10 has a positioning end 11, the positioning end 11 is exposed out of the support frame 20 from the notch 211 of the receiving groove 21, and as shown in fig. 5, a receiving hole 111 is formed in the positioning end 11 extending from the end thereof to the inside thereof.

As shown in FIG. 1, the middle bracket 30 of the clip is located below the support bracket 20, and FIG. 1 shows a portion of the structure of the middle bracket 30 of the clip that carries the seal ring. Specifically, a plurality of loading holes 31 are arranged on the middle bracket 30 of the clip, as shown in fig. 4, fig. 4 is a schematic view of one of the loading holes 31, namely a partial structure of the middle bracket 30 of the clip. Each object carrying hole 31 corresponds to one positioning end 11 of one positioning assembly 10, as shown in fig. 4, the object carrying hole 31 has a sealing ring object carrying portion 311 and a sealing ring through hole portion 312 which are coaxial, the sealing ring object carrying portion 311 is used for carrying the sealing ring 200, the positioning end 11 is used for abutting against the sealing ring 200 in the sealing ring object carrying portion 311 of the clamping middle support 30 so as to position the sealing ring 200 in the sealing ring object carrying portion 311, the sealing ring through hole portion 312 is used for allowing the capillary tube 100 to pass through, and the accommodating hole 111 is used for accommodating the capillary tube 100 passing through from the sealing ring through hole portion 312.

In the above embodiment, it is considered that the support frame 20 is slidably disposed on a sliding mechanism, the middle support 30 of the clip is disposed below the support frame 20, and the receiving hole 111 and the object carrying hole 31 of the positioning end 11 are maintained to be coaxial, the seal ring 200 is placed on the seal ring object carrying portion 311, the support frame 20 is moved in a sliding manner toward the middle support 30 of the clip, so that the positioning end 11 abuts against the seal ring 200 on the seal ring object carrying portion 312, then the capillary tube 100 is inserted through the seal ring passing hole 312 from the other side of the middle support 30 of the clip, which is far away from the seal ring object carrying portion 311, during the insertion process, due to the abutting action of the positioning end 11, the seal ring 200 is ensured not to move along with the movement of the capillary tube 100, the capillary tube 100 is inserted through the seal ring passing hole 312, the seal ring 200 is sleeved on the outer tube wall of the capillary tube 100, and the receiving hole 111 is disposed to provide a moving space for the movement of the capillary tube 100. After the process of inserting the capillary 100 is completed, the support frame 20 can be moved away from the middle support 30 of the clip by sliding.

In one embodiment of the present application, the positioning end 11 is a cylindrical positioning end with a cylindrical shape, an outer diameter of the cylindrical positioning end is smaller than or equal to a diameter of the sealing ring loading part 311, and an aperture of the accommodating hole 111 is smaller than an aperture of the sealing ring through hole part 312, such that the sealing ring loading part 311 and the sealing ring through hole part 312 form a stepped loading hole 31.

In this embodiment, the number of locating component 10 is the same with the number of holding tank 21, all is provided with 12 respectively, and is corresponding, is provided with 12 on the middle part support 30 of clamping and carries thing hole 31, so, can guarantee 12 capillaries 100 simultaneously and wear to locate in corresponding sealing washer 200, compare in the mode that adopts in the past, can effectively improve work efficiency to reduce the cost of labor.

In an embodiment, the groove wall of the accommodating groove 21 can be made of an elastic structure, and the size of the accommodating groove 21 is smaller than that of the positioning assembly 10, so that the positioning assembly 10 can be clamped and positioned in the accommodating groove 21 through the elastic groove wall. In another embodiment, the positioning assembly 10 can be clamped and positioned in the receiving groove 21 of the supporting frame 20 by other methods such as clamping and positioning.

In this embodiment, a clamping and positioning manner is adopted, and as shown in fig. 1, the sealing ring auxiliary positioning device for a capillary tube of a nucleic acid analysis gel column provided in this embodiment further includes: and the clamping assembly 40 is used for clamping and positioning the positioning assemblies 10 in the accommodating grooves 21 of the support frame 20.

As shown in fig. 5, 6 and 8, the support 20 includes: bottom plate 22 and a plurality of baffle 23, a plurality of baffles 23 are all in proper order each other spaced locate bottom plate 22, form holding tank 21 between two adjacent baffles 22, and centre gripping subassembly 40 is installed in bottom plate 22, can fix a position locating component 10 centre gripping in holding tank 21. In this way, the support frame 20 is formed by installing the plurality of partition plates 23 on the bottom plate 22, which facilitates the manufacturing of the support frame.

As shown in fig. 6, in one embodiment, the bottom plate 22 and the partition 23 are fixedly connected by magnetic attraction. In a preferred embodiment, the bottom plate 22 is a magnetically attractable bottom plate made of a magnet, and/or the partition 23 is a magnetically attractable partition made of a magnet. Specifically, bottom plate 22 can be the formula bottom plate of inhaling of magnetism by the preparation of magnet, it is corresponding, baffle 23 can adopt the baffle that steel preparation formed, inhale formula effect through bottom plate 22 and adsorb a location with a plurality of baffles 23, or, baffle 23 can be the formula baffle of inhaling by the magnetism of magnet preparation, bottom plate 22 can adopt the bottom plate that steel was made, inhale formula effect through baffle 23 and adsorb a location with a plurality of channel bars, and adsorb bottom plate 22 and fix a location on baffle 23, or, bottom plate 22 and baffle 23 can all be the formula bottom plate and inhale formula baffle of inhaling by the magnetism of the preparation respectively of magnet, inhale formula effect through respective magnetism and adsorb a location on bottom plate 22 with a plurality of baffles 23, so, in order to improve the packaging efficiency of bottom plate 22 and baffle 23.

As shown in fig. 1, 5 and 6, the clamping assembly 40 includes: the clamping member 41 and the two supporting members 42, the two supporting members 42 are respectively rotatably installed on two opposite sides of the bottom plate 22 through the first rotating shaft 421, and the plurality of partition plates 23 are all disposed between the two opposite sides of the bottom plate 22. The two ends of the clamping member 41 are rotatably mounted between the two supporting members 42 through the second rotating shaft 411, the clamping member 41 is provided with two elastic arms 411, the free ends of the two elastic arms 411 are provided with clamping portions 412, the two opposite sides of the bottom plate 22 are provided with clamping grooves 221, and in this embodiment, the clamping grooves 221 extend along the length direction of the side edges of the two opposite sides of the bottom plate 22. The retaining part 412 is used for retaining in the retaining groove 221 to position the positioning assembly 10 in the receiving groove 21 by the clamping member 41.

In use, the two supporting members 42 rotate around the first rotating shaft 421 to rotate the clamping member 41 to one side of the base plate 22 where the positioning assembly 10 is disposed, so that the clamping member 41 is attached to the positioning assembly 10, the elastic arms 411 move towards two opposite sides of the base plate 22 by an external force, and the clamping parts 412 are clamped in the clamping grooves 221, so that the positioning assembly 10 is clamped and positioned on the partition plate 23 by the clamping member 41 matching with the accommodating groove 21, and the positioning assembly 10 is positioned.

As shown in fig. 7, the retaining part 412 has a first retaining surface 413 inclined from the free end thereof to the connection portion between the retaining part 412 and the elastic arm 411, the retaining groove 221 is provided with a second retaining surface 222 engaged with the first retaining surface 413, when the retaining part 412 is retained in the retaining groove 221, the elastic arm 411 is prevented from being disengaged from the retaining groove 221 by the engagement of the first retaining surface 413 and the second retaining surface 222, and the clamping stability of the clamping member 41 is ensured.

Referring to fig. 6, a buffer member 50 is further disposed at the bottom of each receiving groove 21, and the buffer member 50 is used for buffering the positioning member 10. Specifically, when the clamping member 41 cooperates with the receiving groove 21 to clamp and position the positioning assembly 10 on the partition 23, the positioning assembly can be buffered by the buffering assembly 50 to ensure that the receiving hole 111 of the positioning end 11 is coaxial with the loading hole 31.

In some embodiments, the buffer member 50 may be a buffer column made of rubber or silicone, and fig. 6 shows only one buffer member disposed at the bottom of each receiving groove 21.

As shown in fig. 1, a groove 32 is provided on the middle bracket 30 of the clip, the loading holes 31 are provided at the bottom of the groove 32, and the plurality of loading holes 31 are sequentially arranged at equal intervals along the length direction of the groove 32, the bottom of the groove 32 is further provided with a plurality of separating protrusions 33, and the plurality of separating protrusions 32 are respectively provided between two adjacent loading holes 31.

In this embodiment, the middle support 30 of the clip is a bar-shaped clip, the groove 32 is a bar-shaped groove arranged along the length direction of the bar-shaped clip, the loading holes 31 are sequentially arranged at the bottom of the groove 32 at equal intervals, the plurality of separating protrusions 33 are arranged between two adjacent loading holes 31, and correspondingly, two adjacent separating protrusions 32 are sequentially arranged at equal intervals. As shown in fig. 9, a separation cavity 35 is formed between two adjacent separation protrusions 33, a tapered section 12 is further disposed at one end of the positioning assembly 10, and the separation cavity 35 is disposed so as to cooperate with the tapered section 12 when the positioning end 11 of the positioning assembly 10 moves toward the object carrying hole 31 and abuts against the sealing ring 200, so as to ensure that the positioning end 11 of the positioning assembly 10 can abut against the sealing ring 200.

In summary, the auxiliary positioning device for a sealing ring of a capillary tube of a gel column for nucleic acid analysis provided by this embodiment is configured to dispose a support frame above a middle support of a clip, and keep an accommodation hole of a positioning end coaxial with a carrying hole of a stage, place a sealing ring on a sealing ring carrying portion, move the support frame and make the positioning end of a positioning assembly abut against the sealing ring on the sealing ring carrying portion, so as to abut against the sealing ring on the sealing ring carrying portion, and thread a capillary tube through a hole portion of the sealing ring from the other side of the middle support of the clip, which is away from the sealing ring carrying portion, through the positioning end of the sealing ring. Compared with the mode of only positioning one sealing ring at each time in the prior art, the device can simultaneously position a plurality of sealing rings on the middle support of the nucleic acid analysis gel column, can effectively improve the working efficiency and reduce the labor cost.

The foregoing is a more detailed description of the present application in connection with specific embodiments thereof, and it is not intended that the present application be limited to the specific embodiments thereof. It will be apparent to those skilled in the art from this disclosure that many more simple derivations or substitutions can be made without departing from the inventive concepts herein.

Claims

1. A sealing ring auxiliary positioning device for a nucleic acid analysis gel column capillary tube is characterized by comprising: a plurality of positioning components and a support frame; the supporting frame is provided with a plurality of accommodating grooves, and the end parts of the accommodating grooves are provided with notches communicated with the insides of the accommodating grooves; each positioning assembly corresponds to one accommodating groove respectively, the positioning assemblies are accommodated in the accommodating grooves, each positioning assembly is provided with a positioning end exposed out of the support frame from the notch, and the positioning end extends from the end part to the inside of the positioning end to form an accommodating hole; the locating end is used for abutting against a sealing ring in a sealing ring carrying part of the clamping clamp so as to locate the sealing ring, and the accommodating hole is used for accommodating the capillary tube.

2. The seal ring assisted positioning device for nucleic acid analysis gel column capillaries according to claim 1 further comprising: the clamping assembly is used for clamping and positioning the positioning assemblies in the accommodating grooves of the supporting frame.

3. The seal ring assisted positioning device for a nucleic acid analysis gel column capillary as claimed in claim 2, wherein the support frame comprises: the bottom plate and the plurality of partition plates are sequentially arranged on the bottom plate at intervals, and the accommodating groove is formed between every two adjacent partition plates; the clamping assembly is mounted to the base plate.

4. The seal ring assisted positioning device for nucleic acid analysis gel column capillaries according to claim 3 wherein the clamping assembly comprises: the two supporting pieces are respectively and rotatably arranged on two opposite sides of the bottom plate, and the plurality of partition plates are arranged between the two opposite sides of the bottom plate; the clamping piece is rotatably arranged between the two supporting pieces; the clamping piece is provided with two elastic arms, and the free ends of the two elastic arms are provided with clamping parts; the clamping part is used for clamping the clamping groove, so that the positioning assembly is positioned in the accommodating groove through the clamping piece.

5. The seal ring auxiliary positioning device for a capillary of a nucleic acid analysis gel column according to claim 4, wherein the catching grooves extend in the length direction of the side edges of the opposite sides of the bottom plate.

6. The positioning aid for a sealing ring of a capillary tube in a nucleic acid analysis gel column according to claim 5, wherein said holding portion has a first holding surface inclined from a free end thereof toward a junction of said holding portion and said elastic arm, and said holding groove has a second holding surface engaged with said first holding surface.

7. The seal ring auxiliary positioning device for a capillary of a nucleic acid analysis gel column according to claim 3, wherein the bottom plate is magnetically and fixedly connected to the partition plate.

8. The positioning aid for a sealing ring used in a capillary tube of a nucleic acid analysis gel column according to claim 2, wherein a buffer member for buffering the positioning member is further provided at the bottom of the receiving groove.

9. The positioning aid for a sealing ring of a capillary tube in a nucleic acid analysis gel column according to claim 1, wherein the positioning end is a cylindrical positioning end having a cylindrical shape, an outer diameter of the cylindrical positioning end is equal to or smaller than a diameter of the sealing ring loading portion, and an aperture of the receiving hole is smaller than an aperture of the sealing ring through hole portion.