CN220034449U - Instrument-free portable nucleic acid extraction device - Google Patents

Abstract

The utility model relates to the technical field of nucleic acid extraction, in particular to an instrument-free portable nucleic acid extraction device, which comprises a tube seat and a magnet, wherein the tube seat is provided with an upper surface and a lower surface which are opposite, a tube connecting position is arranged on the lower surface and is used for detachably connecting the upper end of a reagent tube, a magnet connecting position is arranged on the upper surface at a position corresponding to the tube connecting position, the magnet is detachably connected with the magnet connecting position and is opposite to a tube orifice at the upper end of the reagent tube, and the magnet is used for adsorbing magnetic beads in the reagent tube onto the tube seat. The magnet and the reagent tube are respectively and detachably connected with the tube seat, no instrument drive is needed, a complex track drive structure is not needed, the precision problem caused by the matched motion of the power assembly is avoided, the structure is more compact, the volume and the occupied space of the device are greatly reduced, and the device is more convenient to carry; meanwhile, the device does not depend on driving equipment such as a pipette, electric power and the like, is simpler to operate, and can be used for manually completing the rapid extraction of the nucleic acid on site.

Description

Instrument-free portable nucleic acid extraction device

Technical Field

The utility model relates to the technical field of nucleic acid extraction, in particular to an instrument-free portable nucleic acid extraction device.

Background

The isolation and purification of nucleic acids is extremely important in the study of molecular biology and in clinical molecular diagnostics. The quality of nucleic acid extraction in molecular biology research directly affects the subsequent PCR (Polymerase Chain Reaction ), sequencing, data interpretation and the smooth progress of transformation experiments; for the precious clinical samples obtained in molecular diagnosis, whether the high-quality nucleic acid molecules can be extracted is the key for giving the correct analysis and diagnosis results later.

Along with the increasingly important role of gene diagnosis in the diagnosis and monitoring of clinical diseases, the nucleic acid extraction technology, which is a precondition technology for gene diagnosis to be completed, is also newly developed, and the high-efficiency, convenient, large-flux and automatic nucleic acid extraction technology is the main stream direction of the development of the nucleic acid extraction technology. At present, a plurality of brands and types of semi-automatic or full-automatic nucleic acid extraction equipment are available, and the equipment is suitable for users with large detection requirements such as large hospital institutions, disease control centers and the like, but the equipment purchasing cost and the operation cost are high, and the requirements of on-site nucleic acid extraction cannot be met because the equipment is difficult to meet the requirements of an instrument for a resource deficiency area and a mechanism with small detection sample size, the collected sample is transported to a large central laboratory for detection after special preservation, so that a nucleic acid extraction device which is simple to operate and small and portable is needed.

At present, two main methods for extracting nucleic acid are a filter column method and a magnetic bead method. The extraction rate of the filter column method is higher, but the large-scale automatic extraction is difficult to achieve due to the requirement of a centrifugal machine, and the operation is complex, so that the time and the labor are wasted; meanwhile, the requirements on experimental environment are strict. The magnetic bead method can be matched with a nucleic acid extractor for automatic extraction, so that large-batch operation is realized, the operation is simple, and the time is short, so that the magnetic bead method is more and more important for extracting the nucleic acid.

The utility model patent application of publication No. CN114621849A discloses a portable nucleic acid extraction device without an instrument, wherein the relative lifting operation of a magnetic rod and a magnetic sleeve is completed by arranging a special track driving structure. The portable nucleic acid extraction device without the instrument has corresponding use scenes and effects, is relatively complicated to operate, is required to be matched with a magnetic rod sleeve, increases consumable requirements, and meanwhile, the special track driving structure is easy to cause the accuracy of a driving element to be poor due to long-time operation, so that the product performance is influenced.

Disclosure of Invention

In view of the above-mentioned drawbacks of the prior art, an object of the present utility model is to provide an instrument-free portable nucleic acid extraction device that is simpler to operate and does not create precision problems.

In order to solve the technical problems, the utility model adopts the following technical scheme:

the utility model provides an instrument-free portable nucleic acid extraction device, which comprises a tube seat and a magnet, wherein the tube seat is provided with an upper surface and a lower surface which are opposite, a tube connecting position is arranged on the lower surface and is used for detachably connecting the upper end of a reagent tube, the upper surface is provided with a magnet connecting position at a position corresponding to the tube connecting position, the magnet is detachably connected with the magnet connecting position and is opposite to a tube orifice at the upper end of the reagent tube, and the magnet is used for adsorbing magnetic beads in the reagent tube onto the tube seat.

Preferably, the tube connecting position seals the tube opening at the upper end of the reagent tube, the magnet connecting position fixes the magnet and the tube seat, and the tube seat can be arranged vertically or reversely.

Preferably, the tube connection site is connected to the upper end of the reagent tube by screw-fitting.

Preferably, the magnet attachment location is connected to the magnet by an interference fit.

Preferably, a plurality of pipe connection sites are provided on the lower surface of the pipe holder, and a magnet connection site is provided on the upper surface of the pipe holder at a position corresponding to at least one pipe connection site.

Preferably, four tube connection positions are arranged on the lower surface of the tube seat, and the reagent tube comprises a first washing liquid tube pre-filled with a first washing liquid, a second washing liquid tube pre-filled with a second washing liquid and magnetic beads, a cracking liquid tube pre-filled with a cracking liquid and an eluent tube pre-filled with an eluent, wherein the first washing liquid tube, the second washing liquid tube, the cracking liquid tube and the eluent tube are respectively connected with the four tube connection positions.

Preferably, the reagent tube rack further comprises a tube rack for supporting reagent tubes connected to the tube holder.

Preferably, the tube holder is provided with at least one set of first placing holes for placing the reagent tubes connected to one tube holder.

Preferably, the pipe support is provided with a second placing hole and/or a third placing hole, the second placing hole is used for placing the reagent pipe detached from the pipe support, and the third placing hole is used for placing the magnet detached from the pipe support.

Preferably, the pipe support comprises an upper layer plate and a lower layer plate which are oppositely arranged at intervals, the upper layer plate is supported above the lower layer plate through a connecting piece, the upper part of the reagent pipe movably penetrates through the upper layer plate, and the lower end of the reagent pipe is arranged on the lower layer plate.

Compared with the prior art, the utility model has obvious progress:

according to the portable nucleic acid extraction device without the instrument, the magnet and the reagent tube are respectively and detachably connected with the tube seat, the magnet is connected with or detached from the tube seat to realize a magnetic attraction state or a non-magnetic state, no instrument is used for driving, and a complex track driving structure is not needed, so that the precision problem caused by the matched motion of a power assembly is avoided, the structure of the device is more compact, the volume and the occupied space of the nucleic acid extraction device are greatly reduced, and the carrying is more convenient; meanwhile, the instrument-free portable nucleic acid extraction device completely avoids the complex operation of hardware and software of a nucleic acid extraction instrument, does not depend on driving equipment such as a pipettor, electric power and the like, is simpler to operate, can finish the rapid extraction of the nucleic acid on site manually, saves equipment cost, simplifies the processing process and improves the extraction efficiency.

Drawings

FIG. 1 is a schematic front view of an instrument-free portable nucleic acid extraction apparatus according to an embodiment of the present utility model.

FIG. 2 is a schematic top view of an instrument-free portable nucleic acid isolation apparatus according to an embodiment of the present utility model.

Fig. 3 is a schematic cross-sectional view taken along A-A in fig. 2.

FIG. 4 is a schematic diagram showing the separation of a tube holder, a magnet and a reagent tube in the instrument-free portable nucleic acid isolation apparatus according to the embodiment of the present utility model.

FIG. 5 is a schematic view showing a sample loading state of the instrument-free portable nucleic acid isolation apparatus according to the embodiment of the present utility model.

FIG. 6 is a schematic diagram showing a magnetic attraction state of the instrument-free portable nucleic acid isolation apparatus according to the embodiment of the present utility model.

FIG. 7 is a schematic diagram showing a non-magnetic state of the instrument-free portable nucleic acid isolation apparatus according to the embodiment of the present utility model.

Wherein reference numerals are as follows:

1. tube seat 33 cracking liquid tube

1a upper surface 34 eluent tube

1b lower surface 4 pipe frame

11. 11a, 11b, 11c, 11d pipe connection position 41 first placing hole

12. Second placement hole of magnet connecting position 42

2. Third placement hole for magnet 43

21. Flange 4a upper plate

3. Reagent tube 4b lower plate

31. First washing liquid pipe 4c connecting piece

32. Second washing liquid pipe

Detailed Description

The following describes the embodiments of the present utility model in further detail with reference to the accompanying drawings. These embodiments are merely illustrative of the present utility model and are not intended to be limiting.

In the description of the present utility model, it should be noted that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

Furthermore, in the description of the present utility model, unless otherwise indicated, the meaning of "a plurality" is two or more.

As shown in FIGS. 1 to 7, one embodiment of the instrument-free portable nucleic acid extraction apparatus of the present utility model. In the description of the present utility model, the "upper" side is defined as the upper side of the paper of fig. 1, and the "lower" side is defined as the lower side of the paper of fig. 1.

Referring to fig. 1 to 4, the instrument-free portable nucleic acid extraction apparatus of the present embodiment includes a stem 1 and a magnet 2, the stem 1 having opposite upper and lower surfaces 1a and 1b, the upper surface 1a of the stem 1 being a surface facing upward when the stem 1 is in the normal position, and the lower surface 1b of the stem 1 being a surface facing downward when the stem 1 is in the normal position. The lower surface 1b of the tube holder 1 is provided with a tube connecting site 11, and the tube connecting site 11 is used for detachably connecting the upper ends of the reagent tubes 3. The upper surface 1a of the tube holder 1 is provided with a magnet connecting site 12 at a position corresponding to the tube connecting site 11, the magnet 2 is detachably connected to the magnet connecting site 12, and the magnet 2 is opposed to the tube orifice at the upper end of the reagent tube 3, and the magnet 2 is used for adsorbing the magnetic beads in the reagent tube 3 to the tube holder 1. The tube seat 1 is made of a material with magnetic conductivity, and the magnet 2 is preferably a strong magnet.

When the kit is used, the reagent tube 3 filled with reagents and magnetic beads required by nucleic acid extraction is connected to the tube connection position 11 on the lower surface 1b of the tube seat 1, the magnet 2 is connected to the magnet connection position 12 on the upper surface 1a of the tube seat 1, the magnet 2 is opposite to the tube orifice at the upper end of the reagent tube 3, the magnetic beads in the reagent tube 3 can be adsorbed to the tube seat 1 at the tube connection position 11 under the action of the magnet 2, the reagent tube 3 can be disassembled and assembled in a magnetic attraction state at the moment, and the reagent tube 3 filled with different reagents can be replaced according to a nucleic acid extraction flow; when the magnet 2 is detached from the magnet connecting site 12 on the upper surface 1a of the tube holder 1, the magnetic beads adsorbed on the tube holder 1 at the tube connecting site 11 can be released into the reagent tube 3, and in this case, the magnetic beads are in a non-magnetic state, and a corresponding nucleic acid extraction step can be performed. Thus, according to the nucleic acid extraction flow, the magnetic beads can be transferred to different reagents by attaching and detaching the reagent tube 3 and the attaching and detaching magnet 2, thereby realizing the nucleic acid extraction operation.

The magnet 2 and the reagent tube 3 are respectively and detachably connected with the tube seat 1, the magnet 2 is connected with the tube seat 1 or detached and separated to realize a magnetic attraction state or a non-magnetic state, no instrument is used for driving, and a complex track driving structure is not needed, so that the precision problem caused by the matched motion of a power assembly is avoided, the structure of the device is more compact, the volume and the occupied space of the nucleic acid extraction device are greatly reduced, and the device is more convenient to carry; meanwhile, the instrument-free portable nucleic acid extraction device completely avoids complex operations of hardware and software of a nucleic acid extraction instrument, does not depend on driving equipment such as a pipettor and electric power, is simpler to operate, can be used for completing on-site rapid extraction of nucleic acid manually, saves equipment cost, simplifies a processing process, and improves extraction efficiency.

In this embodiment, preferably, when the reagent tube 3 is attached to the tube attachment site 11 on the lower surface 1b of the tube holder 1, the tube attachment site 11 closes the tube opening at the upper end of the reagent tube 3, and when the magnet 2 is attached to the magnet attachment site 12 on the upper surface 1a of the tube holder 1, the magnet attachment site 12 fixes the magnet 2 to the tube holder 1, and the tube holder 1 can be placed upside down or upside down. When the tube holder 1 is in the normal position, referring to fig. 1 and 3, the upper surface 1a of the tube holder 1 faces upward, the lower surface 1b of the tube holder 1 faces downward, and the reagent tube 3 connected to the tube connecting position 11 is in the normal position with its tube mouth facing upward. When the tube holder 1 is inverted, referring to fig. 6 and 7, the upper surface 1a of the tube holder 1 faces downward, the lower surface 1b of the tube holder 1 faces upward, and the reagent tube 3 attached to the tube connecting portion 11 is in an inverted state with its mouth of pipe facing downward. The pipe connection position 11 seals the pipe orifice at the upper end of the reagent pipe 3, so that the reagent in the reagent pipe 3 can be prevented from overflowing from the pipe orifice when the pipe seat 1 is inverted. The magnet connection site 12 fixes the magnet 2 to the socket 1 to prevent the magnet 2 from falling off the magnet connection site 12 when the socket 1 is inverted. Therefore, when in use, the tube seat 1 can be changed to be in an upright state or an inverted state according to the nucleic acid extraction flow, the steps of connecting and detaching the reagent tube 3, connecting the magnet 2 and extracting the nucleic acid in a non-magnetic state after detaching the magnet 2 can be carried out in the upright state of the tube seat 1, and the magnet 2 is adsorbed by the magnet 2 and the magnet 2 is detached to release the magnet bead in the inverted state of the tube seat 1. By inverting the stem 1, the magnetic beads can be better attracted or released to achieve a magnetically attracted state or a non-magnetized state. Of course, the magnet 2 may be detached to release the beads in the state where the stem 1 is in the normal position.

Preferably, the pipe connection position 11 on the lower surface 1b of the pipe seat 1 is connected with the upper end of the reagent pipe 3 through screw thread fit, and has the advantages of convenient disassembly and assembly and good connection tightness. Referring to fig. 3, in this embodiment, the tube connecting position 11 is a blind hole formed by upwardly recessing the lower surface 1b of the tube holder 1, an internal thread is provided on the inner peripheral wall of the blind hole, an external thread is provided on the outer peripheral surface of the upper end of the reagent tube 3, during assembly, the upper end of the reagent tube 3 is inserted into the blind hole, and the external thread of the upper end of the reagent tube 3 is cooperatively connected with the internal thread of the blind hole, thereby realizing that the tube connecting position 11 is detachably connected with the upper end of the reagent tube 3 and sealing the tube orifice of the upper end of the reagent tube 3.

Preferably, the magnet connecting position 12 on the upper surface 1a of the tube seat 1 is connected with the magnet 2 through interference fit, so that the assembly and disassembly are convenient. Referring to fig. 3 and 4, in the present embodiment, the magnet connecting position 12 is a bayonet formed by downwardly recessing the upper surface 1a of the tube holder 1, and when assembled, the bayonet is in interference fit with the lower end of the magnet 2, so that the lower end of the magnet 2 is embedded and fixed in the bayonet, and when disassembled, the magnet 2 is pulled out, thereby realizing the detachable connection of the magnet connecting position 12 and the magnet 2 and fixing the magnet 2 and the tube holder 1.

In the present embodiment, it is preferable that a plurality of pipe connection sites 11 are provided on the lower surface 1b of the pipe socket 1, and a magnet connection site 12 is provided on the upper surface 1a of the pipe socket 1 at a position corresponding to at least one pipe connection site 11. Therefore, a plurality of reagent tubes 3 filled with different reagents can be connected on one tube seat 1 at the same time, the reagent tubes 3 filled with different reagents can be moved to the tube connection positions 11 corresponding to the magnet connection positions 12 according to the nucleic acid extraction process, and the magnet 2 at the magnet connection positions 12 is disassembled to realize the transfer of magnetic beads in different reagents so as to realize the nucleic acid extraction operation.

Preferably, four pipe connection locations 11, namely, a connection pipe location 11a, a connection pipe location 11b, a connection pipe location 11c and a connection pipe location 11d, are provided on the lower surface 1b of the pipe holder 1. The reagent tube 3 includes a first washing liquid tube 31 preloaded with a first washing liquid, a second washing liquid tube 32 preloaded with a second washing liquid and magnetic beads, a lysate tube 33 preloaded with a lysate, and an eluent tube 34 preloaded with an eluent. The first washing liquid pipe 31, the second washing liquid pipe 32, the cleavage liquid pipe 33 and the eluent pipe 34 are connected to the four pipe connection sites 11, respectively. The method comprises the following steps: the second washing liquid pipe 32 is connected to the connection pipe position 11a, the first washing liquid pipe 31 is connected to the connection pipe position 11b, the cleavage liquid pipe 33 is connected to the connection pipe position 11c, and the eluent pipe 34 is connected to the connection pipe position 11d. A magnet coupling position 12 is provided on the upper surface 1a of the stem 1 at a position corresponding to the coupling tube position 11 a. Therefore, the instrument-free portable nucleic acid extraction device of the embodiment has the advantages that each reagent tube 3 for extracting the nucleic acid is pre-packaged, the cracking combination, rinsing and elution processes can be realized in one device, the rapid extraction of the nucleic acid is realized, the carrying is convenient, the operation steps are few, simple, rapid and efficient, the cost is low, the instrument-free portable nucleic acid extraction device is particularly suitable for the services of field sampling, pet disease detection and the like, and the rapid extraction and purification of the nucleic acid sample by customers are facilitated.

Of course, the number of the tube connecting sites 11 on the lower surface 1b of the tube holder 1 is not limited to four, and the number of the magnet connecting sites 12 on the upper surface 1a of the tube holder 1 is not limited to one, and the number of the tube connecting sites 11 and the magnet connecting sites 12 on one tube holder 1 may be increased or decreased depending on the nucleic acid extracting reagent requirements. The number of magnets 2 is not limited, and one or more magnets 2 may be provided according to the number of magnet attachment sites 12.

Further, referring to FIGS. 3 and 5, the instrument-free portable nucleic acid extraction apparatus of the present embodiment further comprises a tube holder 4, the tube holder 4 for supporting the reagent tube 3 attached to the tube holder 1, so that the reagent tube 3 attached to the tube attachment site 11 on the lower surface 1b of the tube holder 1 can be stably placed in the state where the tube holder 1 is in the normal position.

Preferably, in this embodiment, referring to fig. 2 and 5, the tube rack 4 is provided with at least one set of first placement holes 41, and the set of first placement holes 41 is used for placing the reagent tubes 3 connected to one tube holder 1, and the set of first placement holes 41 includes the same number of first placement holes 41 as the tube connection sites 11 on one tube holder 1 and are arranged in a one-to-one correspondence. When the tube rack 4 is provided with a plurality of groups of first placing holes 41, a plurality of tube seats 1, matched magnets 2 and reagent tubes 3 can be arranged, and each tube seat 1 can perform a group of nucleic acid extraction operations, so that a plurality of groups of nucleic acid extraction operations can be performed on one tube rack 4 at the same time.

Preferably, in this embodiment, referring to fig. 5, the tube holder 4 may further be provided with a second placement hole 42, and the second placement hole 42 is used for placing the reagent tube 3 detached from the tube holder 1. The second placing hole 42 serves as a redundant placing hole for temporarily placing the reagent vessel 3 detached from the tube holder 1 so as to facilitate the loading and the like of the reagent vessel 3. The number of the second placement holes 42 is not limited, and one or more may be provided. When a plurality of sets of first placement holes 41 are provided on the pipe rack 4, each set of first placement holes 41 may be provided with at least one second placement hole 42.

Preferably, in this embodiment, referring to fig. 5, a third placement hole 43 may be further provided on the tube holder 4, and the third placement hole 43 is used for placing the magnet 2 detached from the tube holder 1. The third placement hole 43 serves as a magnet placement hole for placing the magnet 2 detached from the stem 1 in a non-magnetic state. The number of the third placing holes 43 is not limited, and one or more may be provided according to the number of the magnets 2. When a plurality of sets of first placement holes 41 are provided on the pipe rack 4, each set of first placement holes 41 may be provided with at least one third placement hole 43. In this embodiment, referring to fig. 4, the upper end of the magnet 2 is provided with a flange 21, and the diameter of the flange 21 is larger than that of the third placement hole 43, so that the magnet 2 can be hung on the third placement hole 43 through the flange 21 when placed in the third placement hole 43.

Preferably, referring to fig. 3 and 5, the tube rack 4 includes an upper plate 4a and a lower plate 4b disposed opposite to each other at a distance, the upper plate 4a is supported above the lower plate 4b by a connector 4c, the upper portion of the reagent tube 3 is movably penetrated through the upper plate 4a, and the lower end of the reagent tube 3 is placed on the lower plate 4 b. In this embodiment, the first placing hole 41, the second placing hole 42 and the third placing hole 43 are through holes which are formed in the upper plate 4a and penetrate the upper plate 4a up and down. The lower end of the reagent tube 3 is supported by the lower layer plate 4b, the upper part of the reagent tube 3 is supported and limited by the upper layer plate 4a, and the reagent tube 3 is prevented from tilting, so that the tube frame 4 can well support the reagent tube 3 connected to the tube seat 1, and the stability of the normal state of the tube seat 1 is ensured. Meanwhile, the pipe support 4 can also be used for supporting the pipe support 1 in an inverted state, and the pipe support 1 can be stably placed on the upper layer plate 4a when being inverted. Referring to fig. 6, in the magnet-attracting state, the magnet 2 attached to the socket 1 may pass through one of the first placement holes 41 in such a manner that the upper surface 1a of the inverted socket 1 is fitted with the upper plate 4a, and the flange 21 of the magnet 2 should have a diameter smaller than that of the first placement hole 41 in order that the magnet 2 attached to the socket 1 may pass through one of the first placement holes 41 in the socket 1 when the socket 1 is inverted. Referring to fig. 7, when the socket 1 is inverted, in a non-magnetic state, the magnet 2 is detached from the socket 1 and placed in the third placing hole 43 on the upper plate 4 a.

In a specific embodiment, in the initial state of the instrument-free portable nucleic acid extraction apparatus of this example, the connection tube position 11a, the connection tube position 11b, the connection tube position 11c, and the connection tube position 11d on the lower surface 1b of the tube holder 1 are respectively connected with the second washing liquid tube 32 preloaded with the second washing liquid and the magnetic beads, the first washing liquid tube 31 preloaded with the first washing liquid, the lysis liquid tube 33 preloaded with the lysis liquid, and the elution liquid tube 34 preloaded with the elution liquid, and the magnet 2 is connected to the magnet connection position 12 provided at the position corresponding to the connection tube position 11a on the upper surface 1a of the tube holder 1, and the procedure of nucleic acid extraction using the instrument-free portable nucleic acid extraction apparatus of this example is sequentially as follows.

1) The lysate pipe 33 connected at the connection pipe position 11c is removed, and the removed lysate pipe 33 is placed in the second placement hole 42 on the pipe rack 4.

2) Inverting the tube holder 1 and placing it on the upper plate 4a of the tube holder 4, and passing the magnet 2 connected to the magnet connecting site 12 through a first placing hole 41 so that the tube holder 1 is stably inverted on the upper plate 4a of the tube holder 4; a magnetic attraction 30s for causing the magnetic beads in the second washing liquid pipe 32 connected at the connection pipe position 11a to be attracted to the pipe holder 1 by the magnet 2; then, the tube holder 1 is set up, the second washing liquid pipe 32 connected to the connection tube position 11a is detached, and the removed second washing liquid pipe 32 is mounted to the connection tube position 11 c.

3) The sample to be extracted and proteinase K are added to the lysate tube 33, and then the lysate tube 33 is mounted at the connection tube site 11 a.

4) The tube holder 1 is inverted, the magnet 2 attached at the magnet attachment site 12 is detached, and the detached magnet 2 is placed in the third placement hole 43 on the tube holder 4, so that the magnetic beads adsorbed on the tube holder 1 by the magnet 2 are released into the lysate in the lysate tube 33.

5) The tube holder 1 is arranged in a right direction, each reagent tube 3 connected to the tube holder 1 passes through a first placing hole 41 on the upper plate 4a of the tube holder 4 and is supported on the lower plate 4b of the tube holder 4, and after the nucleic acid is cracked in the cracking liquid tube 33 for 3min-10min, the magnet 2 is arranged at the magnet connecting position 12.

6) Inverting the tube holder 1 and placing it on the upper plate 4a of the tube holder 4, and passing the magnet 2 connected to the magnet connecting site 12 through a first placing hole 41 so that the tube holder 1 is stably inverted on the upper plate 4a of the tube holder 4; a magnetic attraction 30s for causing the magnetic beads in the lysate tube 33 connected at the connection tube position 11a to be attracted to the tube holder 1 by the magnet 2; then, the tube holder 1 is set up, the lysate tube 33 connected to the connection tube position 11a is removed, and the removed lysate tube 33 is placed in the second placement hole 42 of the tube holder 4.

7) Disassembling the first washing liquid pipe 31 connected at the connection pipe position 11b, and installing the removed first washing liquid pipe 31 at the connection pipe position 11 a; then the tube holder 1 is inverted, the magnet 2 connected to the magnet connecting position 12 is disassembled, and the removed magnet 2 is placed in the third placing hole 43 on the tube holder 4, so that the magnetic beads adsorbed on the tube holder 1 by the magnet 2 are released into the first washing liquid in the first washing liquid tube 31; the tube holder 1 is then placed in the right position, each reagent tube 3 connected to the tube holder 1 is respectively supported on the lower plate 4b of the tube holder 4 through a first placing hole 41 on the upper plate 4a of the tube holder 4, and after rinsing in the first washing liquid tube 31 for 1min, the magnet 2 is mounted at the magnet connecting position 12.

8) Inverting the tube holder 1 and placing it on the upper plate 4a of the tube holder 4, and passing the magnet 2 connected to the magnet connecting site 12 through a first placing hole 41 so that the tube holder 1 is stably inverted on the upper plate 4a of the tube holder 4; a magnetic attraction 30s for causing the magnetic beads in the first washing liquid pipe 31 connected at the connection pipe position 11a to be attracted to the pipe holder 1 by the magnet 2; then, the tube holder 1 is set up, the first washing liquid pipe 31 connected to the connection tube position 11a is detached, and the removed first washing liquid pipe 31 is mounted to the connection tube position 11 b.

9) Disassembling the second washing liquid pipe 32 connected at the connection pipe position 11c, and installing the removed second washing liquid pipe 32 at the connection pipe position 11 a; then the tube seat 1 is inverted, the magnet 2 connected to the magnet connecting position 12 is disassembled, and the removed magnet 2 is placed in the third placing hole 43 on the tube frame 4, so that the magnetic beads adsorbed on the tube seat 1 by the magnet 2 are released into the second washing liquid in the second washing liquid tube 32; the tube holder 1 is then placed in the right position, each reagent tube 3 connected to the tube holder 1 is respectively supported on the lower plate 4b of the tube holder 4 through a first placing hole 41 on the upper plate 4a of the tube holder 4, and after rinsing in the second washing liquid tube 32 for 1min, the magnet 2 is mounted at the magnet connecting position 12.

10 The tube holder 1 is inverted and placed on the upper plate 4a of the tube holder 4, and the magnet 2 attached to the magnet attachment site 12 is passed through a first placement hole 41 so that the tube holder 1 is smoothly inverted on the upper plate 4a of the tube holder 4; a magnetic attraction 30s for causing the magnetic beads in the second washing liquid pipe 32 connected at the connection pipe position 11a to be attracted to the pipe holder 1 by the magnet 2; then, the tube holder 1 is set up, the second washing liquid pipe 32 connected to the connection tube position 11a is detached, and the removed second washing liquid pipe 32 is mounted to the connection tube position 11 c.

11 Air-drying at room temperature for 2-5 min.

12 Disassembling the eluent pipe 34 connected at the connection pipe site 11d, and installing the removed eluent pipe 34 at the connection pipe site 11 a; then the tube holder 1 is inverted, the magnet 2 connected to the magnet connecting position 12 is disassembled, and the removed magnet 2 is placed in the third placing hole 43 on the tube holder 4, so that the magnetic beads adsorbed on the tube holder 1 by the magnet 2 are released into the eluent in the eluent tube 34; the tube holder 1 is then placed in the right position, each reagent tube 3 connected to the tube holder 1 is respectively supported on the lower plate 4b of the tube holder 4 through a first placing hole 41 on the upper plate 4a of the tube holder 4, and after eluting for 1min-3min in the eluent tube 34, the magnet 2 is mounted at the magnet connecting position 12.

13 The tube holder 1 is inverted and placed on the upper plate 4a of the tube holder 4, and the magnet 2 attached to the magnet attachment site 12 is passed through a first placement hole 41 so that the tube holder 1 is smoothly inverted on the upper plate 4a of the tube holder 4; a magnet 30s for allowing the magnetic beads in the eluent tube 34 connected at the connection tube position 11a to be adsorbed onto the tube holder 1 by the magnet 2; the tube holder 1 is then set up and the eluent tube 34 connected to the connection tube position 11a is removed. The removed eluate tube 34 is the extracted nucleic acid product.

Thus, one nucleic acid extraction is completed.

The foregoing is merely a preferred embodiment of the present utility model, and it should be noted that modifications and substitutions can be made by those skilled in the art without departing from the technical principles of the present utility model, and these modifications and substitutions should also be considered as being within the scope of the present utility model.

Claims (10)

1. The utility model provides a no instrument portable nucleic acid extraction device, its characterized in that includes tube socket (1) and magnet (2), tube socket (1) have relative upper surface (1 a) and lower surface (1 b), be equipped with on lower surface (1 b) tube coupling position (11), tube coupling position (11) are used for detachably connecting the upper end of reagent pipe (3), be equipped with magnet coupling position (12) on upper surface (1 a) in the position corresponding to tube coupling position (11), magnet (2) with magnet coupling position (12) detachably connect and with the mouth of pipe of reagent pipe (3) upper end is relative, magnet (2) are used for adsorbing the magnetic bead in reagent pipe (3) on tube socket (1).

2. The instrument-free portable nucleic acid extraction apparatus according to claim 1, wherein the tube connecting portion (11) closes a tube opening at an upper end of the reagent tube (3), the magnet connecting portion (12) fixes the magnet (2) to the tube holder (1), and the tube holder (1) can be placed in an upright position or inverted position.

3. The instrument-free portable nucleic acid extraction apparatus according to claim 2, wherein the tube connection site (11) is connected to the upper end of the reagent tube (3) by screw-fitting.

4. The instrument-free portable nucleic acid extraction device of claim 2, wherein the magnet attachment site (12) is attached to the magnet (2) by an interference fit.

5. The instrument-free portable nucleic acid extraction apparatus according to claim 1, characterized in that a plurality of the tube connecting sites (11) are provided on a lower surface (1 b) of the tube holder (1), and the magnet connecting sites (12) are provided on an upper surface (1 a) of the tube holder (1) at positions corresponding to at least one of the tube connecting sites (11).

6. The instrument-free portable nucleic acid extraction apparatus according to claim 5, wherein four of the tube connecting sites (11) are provided on the lower surface (1 b) of the tube holder (1), the reagent tube (3) comprises a first washing liquid tube (31) preloaded with a first washing liquid, a second washing liquid tube (32) preloaded with a second washing liquid and magnetic beads, a lysate tube (33) preloaded with a lysate, and an eluent tube (34) preloaded with an eluent, and the first washing liquid tube (31), the second washing liquid tube (32), the lysate tube (33), and the eluent tube (34) are connected to the four tube connecting sites (11), respectively.

7. The instrument-free portable nucleic acid extraction apparatus according to any one of claims 1 to 6, further comprising a tube holder (4), the tube holder (4) for supporting the reagent tube (3) attached to the tube holder (1).

8. The instrument-free portable nucleic acid extraction apparatus according to claim 7, wherein the tube holder (4) is provided with at least one set of first placement holes (41), and one set of the first placement holes (41) is used for placing the reagent tube (3) attached to one of the tube holders (1).

9. The instrument-free portable nucleic acid extraction apparatus according to claim 7, wherein a second placement hole (42) and/or a third placement hole (43) are further provided in the tube holder (4), the second placement hole (42) being for placing the reagent tube (3) detached from the tube holder (1), and the third placement hole (43) being for placing the magnet (2) detached from the tube holder (1).

10. The instrument-free portable nucleic acid extraction apparatus according to claim 7, wherein the tube rack (4) comprises an upper plate (4 a) and a lower plate (4 b) which are disposed in opposition at an interval, the upper plate (4 a) is supported above the lower plate (4 b) by a connector (4 c), the upper portion of the reagent tube (3) is movably penetrated through the upper plate (4 a), and the lower end of the reagent tube (3) is disposed on the lower plate (4 b).