CN214004651U - Nucleic acid extraction device - Google Patents
Nucleic acid extraction device Download PDFInfo
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- CN214004651U CN214004651U CN202022623275.4U CN202022623275U CN214004651U CN 214004651 U CN214004651 U CN 214004651U CN 202022623275 U CN202022623275 U CN 202022623275U CN 214004651 U CN214004651 U CN 214004651U
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Abstract
The utility model belongs to the technical field of nucleic acid preparation, a nucleic acid extraction device is disclosed, including the work carrier and locate the digital micro-fluidic chip of the upper surface of work carrier, be equipped with gene extract and magnetic bead in the digital micro-fluidic chip, adopt the digital micro-fluidic chip to carry out nucleic acid extraction in order to realize relevant detection, have advantages such as small volume and high flux, the nucleic acid extraction device still includes the magnetism module of inhaling that locates the below of work carrier, magnetism module of inhaling includes magnetism and inhales subassembly and lifting unit, magnetism is inhaled the subassembly and is corresponded the setting from top to bottom with the digital micro-fluidic chip, can effectively reduce the upper strata size of whole device; the magnetic attraction component is designed for the digital microfluidic chip in a matched mode, the lifting component is used for driving the magnetic attraction component to be close to or far away from the kit, magnetic beads are attracted or released through magnetic attraction, operations such as cracking, washing, rinsing and eluting are completed, automatic extraction of nucleic acid is achieved, the magnetic attraction component does not extend into the digital microfluidic chip, reagents are prevented from being polluted, and detection accuracy is improved.
Description
Technical Field
The utility model relates to a nucleic acid preparation technical field especially relates to a nucleic acid extraction element.
Background
Nucleic acid is a carrier of genetic information, is the most important biological information molecule and is the main object of molecular biology research, therefore, the extraction of nucleic acid is the most important and basic operation in molecular biology experimental technology. The magnetic bead method is an important nucleic acid extraction method, and the basic principle is that nucleic acid after cell lysis is adsorbed by magnetic beads, and then the purified nucleic acid is obtained through washing, rinsing, eluting and other operations.
Generally, a magnetic pen is held by a hand, the magnetic pen is close to or far away from the outer side of a centrifugal tube filled with a mixed liquid of magnetic beads, nucleic acid and a reagent, the magnetic pen adsorbs or releases the magnetic beads so as to realize operations such as cracking, washing, rinsing, elution and the like, and the manual operation mode obviously cannot meet the automatic requirement. Also have the mode that adopts arm cooperation bar magnet to carry out nucleic acid extraction now, arm drive bar magnet goes up and down to make the bar magnet stretch into or stretch out the centrifuging tube, with the stirring or adsorb the magnetic bead, accomplish nucleic acid extraction. However, the magnetic rod and the protective device on the outer layer of the magnetic rod can contact with the reagent, which may cause pollution to the extraction system, and may also cause loss of nucleic acid during separation, which affects the accuracy of the detection result.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a nucleic acid extraction element can realize the automatic of nucleic acid and draw, and the testing result is more accurate, and the upper dimension of device is less.
In order to realize the purpose, the following technical scheme is provided:
the utility model provides a nucleic acid extraction element, including work microscope carrier and set up in the digital micro-fluidic chip of the upper surface of work microscope carrier, be provided with gene extract and magnetic bead in the digital micro-fluidic chip, nucleic acid extraction element still including set up in the module is inhaled to magnetism of the below of work microscope carrier, the module includes is inhaled to magnetism:
the magnetic suction component is arranged corresponding to the digital microfluidic chip up and down;
and the lifting component is used for driving the magnetic attraction component to be close to or far away from the digital microfluidic chip so as to magnetically attract or release magnetic beads.
As a preferred embodiment of the nucleic acid extracting apparatus of the present invention, the elevating assembly comprises:
a lifting drive member;
the screw rod is connected with the output end of the lifting driving piece, the nut is connected with the magnetic suction assembly, the nut is sleeved on the screw rod, and the lifting driving piece drives the nut to move along the length direction of the screw rod.
As a preferable scheme of the nucleic acid extracting apparatus of the present invention, the lifting assembly further includes a transmission assembly, the transmission assembly includes a gear set, an input end of the gear set is connected to an output end of the lifting driving member, and an output end of the gear set is coaxially connected to the screw rod; or the like, or, alternatively,
the transmission assembly comprises a first belt wheel, a second belt wheel and a conveying belt, the first belt wheel is connected with the second belt wheel through the conveying belt, the first belt wheel is connected with the output end of the lifting driving piece, and the second belt wheel is coaxially connected with the screw rod.
As a preferable embodiment of the nucleic acid extraction apparatus of the present invention, the lifting unit further includes:
the lifting driving piece and the screw rod are arranged on the fixing frame;
the mounting bracket is connected with the nut, and the magnetic suction module is arranged on the mounting bracket.
As the utility model discloses a preferred scheme of nucleic acid extraction element, lifting unit still including set up in guide pillar on the mount, be provided with the guide pin bushing on the mount bracket, the guide pillar runs through the guide pin bushing, it is right to inhale the elevating movement of subassembly and lead.
As an optimized proposal of the nucleic acid extracting device of the utility model, the nucleic acid extracting device also comprises a limit component, the limit component is used for limiting the magnetic attraction component is close to the displacement of the motion of the digital micro-fluidic chip.
As a preferred embodiment of the nucleic acid extracting apparatus of the present invention, the limiting member comprises:
the first limiting piece is arranged on the fixed frame;
the second limiting part is arranged on the mounting rack, the second limiting part and the first limiting part are arranged in a vertically opposite mode, and when the second limiting part is abutted against the first limiting part, the magnetic attraction component ascends in place and magnetically attracts magnetic beads.
As a preferred scheme of the nucleic acid extraction element of the utility model, first locating part with be provided with the recess on one of the second locating part, first locating part with be provided with the arch on another in the second locating part, when the subassembly rises to target in place is inhaled to magnetism, protruding card is gone into in the recess.
As the utility model discloses a preferred scheme of nucleic acid extraction element, magnetism is inhaled the subassembly and is inhaled piece and support frame, a plurality of including magnetism inhale the piece all set up in on the support frame.
As an optimized proposal of the nucleic acid extracting device of the utility model, the magnetic attraction piece is a magnet.
The utility model has the advantages that:
the utility model provides an among the nucleic acid extraction device, be provided with gene extract and magnetic bead in the digital micro-fluidic chip, adopt the digital micro-fluidic chip to carry out nucleic acid extraction in order to realize relevant detection, have advantages such as small volume and high flux, inhale the subassembly through lifting unit drive magnetism simultaneously and be close to or keep away from the digital micro-fluidic chip, make magnetism inhale the module and adsorb or release the magnetic bead, with accomplish operations such as schizolysis, washing, rinsing and elution, realize the automation of nucleic acid and draw, inhale the magnetic bead with current magnetic rod magnetism simultaneously and compare, the utility model discloses a magnetism inhale in the module can not stretch into the digital micro-fluidic chip, avoid magnetism inhale the module and contact with reagent and pollute reagent, improve the accuracy of testing result. In addition, the magnetic module is arranged below the working carrier, so that the upper layer size of the whole device, namely the structural size above the working carrier, can be effectively reduced, and can be embedded in a base for supporting the nucleic acid extraction device, so that the size of the whole device is smaller. Through setting up spacing subassembly, control magnetism is inhaled subassembly elevating movement's stroke, avoids magnetism to inhale subassembly collision work microscope carrier or digital micro-fluidic chip, perhaps avoids magnetism to inhale subassembly and work microscope carrier or digital micro-fluidic chip's collision impact force too big. The utility model provides a nucleic acid extraction element has mainly designed magnetism to the supporting module of inhaling of digital micro-fluidic chip, can avoid the reagent that outside stirring part caused to pollute and reagent loss, realizes the automation of equipment simultaneously, and magnetism inhale the module and set up in the below of work microscope carrier, the miniaturization of the whole equipment of being convenient for, the module lift is inhaled to lifting unit drive magnetism, and the elevating speed is fast, improves nucleic acid extraction efficiency.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required to be used in the description of the embodiments of the present invention will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the contents of the embodiments of the present invention and the drawings without creative efforts.
Fig. 1 is a first perspective view of a magnetic module according to an embodiment of the present invention;
fig. 2 is a second axial view of the magnetic module according to the embodiment of the present invention;
fig. 3 is an exploded view of a magnetic module according to an embodiment of the present invention;
fig. 4 is a first process of performing a cracking operation according to an embodiment of the present invention;
fig. 5 is a second process of performing a cracking operation according to an embodiment of the present invention;
fig. 6 is a first process of performing a washing operation according to an embodiment of the present invention;
fig. 7 is a second process of performing a washing operation according to an embodiment of the present invention;
fig. 8 is a first process of performing a rinsing operation according to an embodiment of the present invention;
fig. 9 is a second process of performing a rinsing operation according to an embodiment of the present invention;
fig. 10 is a first process of performing an elution operation provided by an embodiment of the present invention;
fig. 11 is a second process of performing an elution operation according to an embodiment of the present invention.
Reference numerals:
1. a magnetic component; 2. a lifting assembly; 3. a limiting component;
11. a support frame; 12. a magnetic member;
21. a lifting drive member; 22. a screw rod; 23. a nut; 231. a flange; 24. a transmission assembly; 25. a fixed mount; 251. an upper bracket; 252. a base; 26. a mounting frame; 261. avoiding holes; 262. a guide sleeve; 27. a guide post;
31. a first limit piece; 32. a second limiting member;
311. a groove; 321. a protrusion;
100. a digital microfluidic chip;
1a, magnetic beads; 2a, sample-magnetic bead-lysis mixed liquor; 3a, cracking waste liquid; 4a, magnetic bead-washing mixed liquor; 5a, washing waste liquid; 6a, magnetic bead-rinsing mixed liquor; 7a, rinsing waste liquid; 8a, magnetic bead-elution mixed liquor; 9a, DNA-containing eluent.
Detailed Description
In order to make the technical problems, technical solutions adopted and technical effects achieved by the present invention clearer, the embodiments of the present invention will be described in further detail with reference to the accompanying drawings, and obviously, the described embodiments are only some embodiments, not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by the skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Wherein the terms "first position" and "second position" are two different positions.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection or a removable connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
As shown in fig. 1 to fig. 3, the present embodiment provides a nucleic acid extraction device, which includes a work stage and a digital microfluidic chip disposed on an upper surface of the work stage. Magnetic beads are arranged in the digital microfluidic chip. Certainly, the digital microfluidic chip is also internally provided with a gene extracting solution, and the magnetic beads are used for adsorbing nucleic acid after cell lysis. The nucleic acid extraction device also comprises a magnetic suction module arranged below the working carrier. The magnetic module comprises a magnetic component 1 and a lifting component 2. The magnetic suction component 1 and the digital microfluidic chip are arranged up and down correspondingly. The lifting component 2 is used for driving the magnetic attraction component 1 to be close to or far away from the digital microfluidic chip so as to magnetically attract or release magnetic beads. The limiting component 3 is used for limiting the displacement of the magnetic suction component 1 close to the movement of the digital microfluidic chip.
The digital PCR technology is a technology which directly counts and analyzes the amplification result in a positive or recessive way by performing high-power dilution and liquid separation on a trace sample to ensure that each reaction unit contains no more than one template molecule, and then performing PCR amplification on all samples. The digital PCR technology is realized by adopting a digital microfluidic chip. The digital microfluidic chip integrates basic operation units such as sample preparation, reaction, separation, detection and the like in the fields of chemistry, biology and the like on a very small chip, and a network is formed by microchannels, so that controllable fluid penetrates through the whole system and is used for realizing various functions of a conventional chemical or biological laboratory. The embodiment adopts the digital microfluidic chip to extract nucleic acid, and has the advantages of small volume, high flux and the like.
Further, the magnetic module is designed for the digital microfluidic chip. Wherein, magnetism is inhaled the module and is inhaled subassembly 1 and lifting unit 2 including magnetism. Magnetic subassembly 1 is inhaled through lifting unit 2 drive magnetism and is close to or keep away from digital micro-fluidic chip, makes magnetism inhale the module and adsorb or release the magnetic bead to accomplish operations such as schizolysis, washing, rinsing and elution, realize the automation of nucleic acid and draw, compare with current magnetic rod magnetism magnetic bead, the module is inhaled to magnetism of this embodiment does not stretch into digital micro-fluidic chip in, avoids magnetism to inhale the module and contacts with reagent and pollute reagent, improves the accuracy of testing result. In addition, the magnetic module is arranged below the working carrier, so that the upper layer size of the whole device, namely the structural size above the working carrier, can be effectively reduced, and can be embedded in a base for supporting the nucleic acid extraction device, so that the size of the whole device is smaller. Through setting up spacing subassembly 3, the stroke of the ascending motion of subassembly 1 is inhaled to control magnetism, avoids magnetism to inhale subassembly 1 collision work microscope carrier or digital micro-fluidic chip, perhaps avoids magnetism to inhale subassembly 1 and work microscope carrier or digital micro-fluidic chip's collision impact force too big.
The work carrier may be provided with a through stepped hole, and the stepped hole includes a first hole and a second hole that communicate with each other. The first aperture is larger in size than the second aperture. The digital microfluidic chip is placed in the first hole. The magnetic component 1 can extend into the second hole and even contact with the bottom of the digital microfluidic chip, so that sufficient magnetic attraction force can be generated between the magnetic component 1 and the magnetic beads. Or, do not set up the pore structure that runs through on the work microscope carrier, when the distance between subassembly 1 and the magnetic bead is inhaled to magnetism equals to or even is greater than the thickness of work microscope carrier, the magnetic bead still can be effectively inhaled to the subassembly 1 of inhaling to magnetism.
In this embodiment, the lifting assembly 2 includes a lifting driving member 21, and a screw rod 22 and a nut 23 which are engaged with each other. The nut 23 is threadedly engaged with the lead screw 22. The screw rod 22 is connected with the output end of the lifting driving piece 21, and the nut 23 is connected with the magnetic suction component 1. The elevation driving member 21 is preferably a motor, a cylinder, or the like.
The output end of the lifting driving part 21 is controlled to move along a first direction, the lifting driving part 21 drives the screw rod 22 to rotate around the axis of the screw rod 22 along a second direction, the screw rod 22 further drives the nut 23 to ascend along the length direction of the screw rod 22, and the magnetic attraction component 1 moves along with the nut 23 and is close to the digital microfluidic chip until the magnetic attraction component 1 magnetically attracts magnetic beads in the digital microfluidic chip. The output end of the lifting driving part 21 is controlled to move along a third direction opposite to the first direction, the lifting driving part 21 drives the screw rod 22 to rotate around the axis of the screw rod 22 along a fourth direction opposite to the second direction, the screw rod 22 further drives the nut 23 to move downwards along the length direction of the screw rod 22, the magnetic attraction component 1 moves along with the nut 23 and is far away from the digital microfluidic chip until the distance between the magnetic attraction component 1 and the magnetic bead is larger than the acting distance of the magnetic attraction between the magnetic attraction component 1 and the magnetic bead, and the magnetic attraction component 1 releases the magnetic bead.
The lifting assembly 2 further comprises a transmission assembly 24. Illustratively, the transmission assembly 24 includes a gear set, an input end of the gear set is connected with an output end of the lifting driving member 21, and an output end of the gear set is coaxially connected with the screw rod 22, so as to realize the transmission connection between the lifting driving member 21 and the screw rod 22. Or, the transmission assembly 24 includes a first belt wheel, a second belt wheel and a transmission belt, the first belt wheel is connected with the second belt wheel through the transmission belt, the first belt wheel is connected with the output end of the lifting driving member 21, and the second belt wheel is coaxially connected with the screw rod 22. The lifting drive 21 is connected to the spindle 22 by belt drive. Of course, the transmission assembly 24 may also be other conventional transmission mechanisms, which are not listed here.
Further, the lifting assembly 2 further comprises a fixed frame 25 and a mounting frame 26. The lifting driving member 21 and the screw rod 22 are both arranged on the fixing frame 25. Mounting bracket 26 is connected with nut 23, and the module setting is inhaled to magnetism on mounting bracket 26. In this embodiment, the fixing member is frame-shaped, and includes an upper bracket 251 and a U-shaped base 252 disposed up and down. The upper holder 251 may be detachably coupled to the base 252 by bolts. The mounting frame 26 is plate-shaped, and is provided with the avoidance hole 261 on it, and nut 23 passes the avoidance hole 261, and nut 23 epirelief is equipped with flange 231, and flange 231 passes through bolt and upper bracket 251 fixed connection.
The magnetic attraction component 1 further comprises a support frame 11, and the support frame 11 is connected to the mounting frame 26 through bolts. The magnetic attraction pieces 12 are disposed on the support frame 11. Illustratively, the magnetically attractive element 12 is a magnet. In this embodiment, the supporting frame 11 is provided with three magnetic attracting elements 12. The support frame 11 is provided with a plurality of lightening holes to reduce the weight of the support frame 11 and avoid the nut 23 bearing too much, so that the wear of the matching threads on the nut 23 and the screw rod 22 is serious, the service life of the nut 23 and the screw rod 22 is influenced, and the light weight of the whole device is facilitated.
In one embodiment, the lifting assembly 2 further includes a guide post 27 disposed on the fixed frame 25. Be provided with guide pin bushing 262 on the mounting bracket 26, guide pillar 27 runs through guide pin bushing 262 to the elevating movement to the subassembly 1 is inhaled to magnetism leads, improves the elevating movement's of subassembly 1 stationarity of inhaling magnetism, guarantees that it can effectively inhale the magnetic bead. The upper and lower ends of the guide post 27 are connected to the upper bracket 251 and the base 252, respectively. Illustratively, two guide posts 27 are provided, and the two guide posts 27 are symmetrically arranged on two sides of the magnetic attraction component 1.
The limiting assembly 3 includes a first limiting member 31 and a second limiting member 32. The first limiting member 31 is disposed on the fixing frame 25. The second limiting part 32 is disposed on the mounting block 26, the second limiting part 32 and the first limiting part 31 are disposed opposite to each other, when the second limiting part 32 abuts against the first limiting part 31, the magnetic attraction component 1 rises in place and magnetically attracts magnetic beads, so that the magnetic attraction component 1 is prevented from colliding with a work stage or a digital microfluidic chip, or the magnetic attraction component 1 is prevented from colliding with the work stage or the digital microfluidic chip with too large impact force.
In this embodiment, the first limiting member 31 is provided with a groove 311, the second limiting member 32 is provided with a protrusion 321, and when the magnetic attraction assembly 1 is lifted to a proper position, the protrusion 321 is clamped into the groove 311, so as to improve the limiting effect. Of course, in other embodiments, the positions of the protrusion 321 and the groove 311 may be interchanged, that is, the protrusion 321 is disposed on the first limiting member 31, and the groove 311 is disposed on the second limiting member 32.
Referring to fig. 4 to fig. 11, the nucleic acid extraction operation steps of the nucleic acid extraction apparatus provided in this embodiment are roughly:
referring to FIGS. 4 and 5, a lysis operation is first performed to isolate DNA. Referring to fig. 4, the first process of the cracking operation is: the sample-magnetic bead-lysis mixed solution 2a is injected into the chip 100, the lysis solution moves at a constant speed on the surface of the electrode according to a specified path, so that the sample is fully lysed to release DNA and is adsorbed by the magnetic bead 1a, the magnetic component 1 of the magnetic module is always far away from the chip 100 in the process, namely the distance between the magnetic component 1 and the magnetic bead 1a is larger than the acting distance of the magnetic force between the magnetic component 1 and the magnetic bead 1 a. Referring to fig. 5, the second process of the cracking operation is: the magnetic component 1 rises to be close to the chip 100, the magnetic bead 1a is adsorbed by the magnetic component 1, the magnetic bead 1a is agglomerated, and the liquid drop is moved to take away the lysis waste liquid 3a containing proteins and cell walls and the like except the DNA adsorbed by the magnetic bead 1 a.
Referring to fig. 6 and 7, a washing operation is performed to remove impurities. FIG. 6 shows a first process of the washing operation, in which the magnetic component 1 descends and is away from the chip 100 until the distance between the magnetic component 1 and the magnetic bead 1a is greater than the distance between the magnetic component and the magnetic bead 1a, a washing solution is added to the chip 100, the magnetic bead 1a and the washing solution are mixed to form a magnetic bead-washing mixture 4a, and the washing solution moves at a constant speed on the surface of the electrode according to a specified path, so that the magnetic bead 1a with DNA adsorbed on the surface is sufficiently washed to remove impurities. FIG. 7 shows a second process of the washing operation, in which the magnetic attraction component 1 is lifted to approach the chip 100, the magnetic attraction component 1 adsorbs the magnetic beads 1a, the magnetic beads 1a are agglomerated, and the liquid drops are moved to take away the washing waste liquid 5a containing the washing liquid and impurities.
Referring to fig. 8 and 9, a rinsing operation is performed to sufficiently remove impurities. Fig. 8 shows a first process of the rinsing operation, in which the magnetic component 1 descends away from the chip 100 until the distance between the magnetic component 1 and the magnetic bead 1a is greater than the acting distance of the magnetic force between the magnetic component and the magnetic bead 1a, and a rinsing liquid is added to the chip 100, and the magnetic bead 1a and the rinsing liquid are mixed to form a magnetic bead-rinsing mixed solution 6 a. The rinsing liquid moves at a constant speed on the surface of the electrode according to a specified path, so that the magnetic beads 1a with DNA adsorbed on the surface are sufficiently cleaned to remove impurities. Fig. 9 shows a second process of the rinsing operation, in which the magnetic attraction component 1 rises close to the chip 100, the magnetic attraction component 1 adsorbs the magnetic beads 1a, the magnetic beads 1a are agglomerated, and the liquid drops are moved to take away the rinsing waste liquid 7a containing the rinsing liquid and the impurities.
Referring to FIGS. 10 and 11, an elution operation is performed to isolate DNA. Fig. 10 shows a first process of the elution operation, in which the magnetic component 1 descends and is away from the chip 100 until the distance between the magnetic component 1 and the magnetic bead 1a is greater than the distance between the magnetic components, and an eluent is added into the chip 100, and the magnetic bead 1a and the eluent are mixed to form a magnetic bead-elution mixed solution 8 a. The eluent moves at a constant speed on the surface of the electrode according to a designated path, so that the DNA adsorbed on the surface of the magnetic bead 1a is separated. FIG. 11 shows a second process of the elution operation, in which the magnetic assembly 1 is lifted close to the chip 100, the magnetic assembly 1 adsorbs the magnetic beads 1a, the magnetic beads 1a are agglomerated, and the droplets are moved to carry away the DNA-containing eluate 9a mixed with DNA, which is used as a reaction sample for a subsequent experiment.
It should be noted that the gene extract solution includes reagents used in four steps (i.e., lysis, washing, rinsing, and elution) of the nucleic acid extraction operation.
In the nucleic acid extraction device that this embodiment provided, adopt digital micro-fluidic chip to carry out nucleic acid extraction, in order to realize various detections, have advantages such as small and high flux, inhale subassembly 1 through lifting unit 2 drive magnetism simultaneously and be close to or keep away from digital micro-fluidic chip, make magnetism inhale the module and adsorb or release the magnetic bead, in order to accomplish operations such as schizolysis, washing, rinsing and elution, realize the automation of nucleic acid and draw, magnetism inhales the module simultaneously and does not stretch into in the digital micro-fluidic chip, magnetism inhales the module and can not pollute the reagent with the reagent contact, improve the accuracy of testing result. In addition, the magnetic module is arranged below the working carrier, so that the upper layer size of the whole device, namely the structural size above the working carrier, can be effectively reduced, and can be embedded in a base for supporting the nucleic acid extraction device, so that the size of the whole device is smaller. Through setting up spacing subassembly 3, the stroke of the ascending motion of subassembly 1 is inhaled to control magnetism, avoids magnetism to inhale subassembly 1 collision work microscope carrier or digital micro-fluidic chip, perhaps avoids magnetism to inhale subassembly 1 and work microscope carrier or digital micro-fluidic chip's collision impact force too big.
It should be noted that the foregoing is only a preferred embodiment of the present invention and the technical principles applied. It will be understood by those skilled in the art that the present invention is not limited to the particular embodiments described herein, but is capable of various obvious changes, rearrangements and substitutions as will now become apparent to those skilled in the art without departing from the scope of the invention. Therefore, although the present invention has been described in greater detail with reference to the above embodiments, the present invention is not limited to the above embodiments, and may include other equivalent embodiments without departing from the scope of the present invention.
Claims (10)
1. The utility model provides a nucleic acid extraction element, its characterized in that, including work microscope carrier and set up in the digital micro-fluidic chip of the upper surface of work microscope carrier, be provided with gene extract and magnetic bead in the digital micro-fluidic chip, nucleic acid extraction element still including set up in magnetism of the below of work microscope carrier is inhaled the module, magnetism is inhaled the module and is included:
the magnetic suction component (1) is arranged corresponding to the digital microfluidic chip up and down;
and the lifting component (2) is used for driving the magnetic attraction component (1) to be close to or far away from the digital microfluidic chip so as to magnetically attract or release magnetic beads.
2. The nucleic acid extraction apparatus according to claim 1, wherein the lifting unit (2) comprises:
a lifting drive member (21);
lead screw (22) and nut (23), lead screw (22) with the output of lift driving piece (21) is connected, nut (23) with magnetism is inhaled the subassembly (1) and is connected, nut (23) cover is located on lead screw (22), lift driving piece (21) pass through lead screw (22) drive nut (23) are followed the length direction of lead screw (22) removes.
3. The nucleic acid extraction device according to claim 2, wherein the lifting assembly (2) further comprises a transmission assembly (24), the transmission assembly (24) comprises a gear set, an input end of the gear set is connected with an output end of the lifting driving member (21), and an output end of the gear set is coaxially connected with the screw rod (22); or the like, or, alternatively,
the transmission assembly (24) comprises a first belt wheel, a second belt wheel and a conveying belt, the first belt wheel is connected with the second belt wheel through the conveying belt, the first belt wheel is connected with the output end of the lifting driving piece (21), and the second belt wheel is coaxially connected with the screw rod (22).
4. The nucleic acid extraction apparatus according to claim 2, wherein the lifting assembly (2) further comprises:
the lifting driving piece (21) and the screw rod (22) are arranged on the fixing frame (25);
the mounting rack (26), the mounting rack (26) with nut (23) are connected, the module of inhaling magnetically set up in on mounting rack (26).
5. The nucleic acid extraction device according to claim 4, wherein the lifting assembly (2) further comprises a guide post (27) disposed on the fixing frame (25), a guide sleeve (262) is disposed on the mounting frame (26), and the guide post (27) penetrates through the guide sleeve (262) to guide the lifting movement of the magnetic attraction assembly (1).
6. The nucleic acid extraction device according to claim 4, further comprising a limiting component (3), wherein the limiting component (3) is used for limiting the displacement of the magnetic attraction component (1) close to the motion of the digital microfluidic chip.
7. The nucleic acid extraction apparatus according to claim 6, wherein the stopper assembly (3) comprises:
the first limiting piece (31) is arranged on the fixed frame (25);
the second limiting part (32) is arranged on the mounting rack (26), the second limiting part (32) and the first limiting part (31) are arranged in a vertically opposite mode, and when the second limiting part (32) is abutted to the first limiting part (31), the magnetic attraction component (1) ascends to the right position and magnetically attracts magnetic beads.
8. The nucleic acid extraction device according to claim 7, wherein one of the first limiting member (31) and the second limiting member (32) is provided with a groove (311), the other of the first limiting member (31) and the second limiting member (32) is provided with a protrusion (321), and when the magnetic attraction component (1) is lifted to a proper position, the protrusion (321) is clamped in the groove (311).
9. The nucleic acid extraction device according to any one of claims 1 to 8, wherein the magnetic attraction component (1) comprises a magnetic attraction member (12) and a support frame (11), and a plurality of the magnetic attraction members (12) are arranged on the support frame (11).
10. The nucleic acid extraction device according to claim 9, wherein the magnetic member (12) is a magnet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022623275.4U CN214004651U (en) | 2020-11-13 | 2020-11-13 | Nucleic acid extraction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022623275.4U CN214004651U (en) | 2020-11-13 | 2020-11-13 | Nucleic acid extraction device |
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| Publication Number | Publication Date |
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| CN214004651U true CN214004651U (en) | 2021-08-20 |
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| CN202022623275.4U Active CN214004651U (en) | 2020-11-13 | 2020-11-13 | Nucleic acid extraction device |
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| CN (1) | CN214004651U (en) |
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