CN111013689A - Electromagnetic force support of nucleic acid extraction centrifuge tube - Google Patents
Electromagnetic force support of nucleic acid extraction centrifuge tube Download PDFInfo
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- CN111013689A CN111013689A CN202010082445.4A CN202010082445A CN111013689A CN 111013689 A CN111013689 A CN 111013689A CN 202010082445 A CN202010082445 A CN 202010082445A CN 111013689 A CN111013689 A CN 111013689A
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- support
- vertical support
- fixing hole
- transverse support
- small holes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/06—Test-tube stands; Test-tube holders
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/20—Mixing the contents of independent containers, e.g. test tubes
- B01F31/201—Holders therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/20—Mixing the contents of independent containers, e.g. test tubes
- B01F31/27—Mixing the contents of independent containers, e.g. test tubes the vibrations being caused by electromagnets
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
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- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Electromagnetism (AREA)
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses an electromagnetic force support of a nucleic acid extraction centrifugal tube, which comprises a vertical support, an upper transverse support, an upper fixing hole, a lower transverse support, a lower fixing hole, an electromagnetic device, a switch and a power socket, wherein the vertical support is a rectangular box body formed by plates, 6 small holes are uniformly distributed on two sides below the vertical support, the total number of the small holes is 12, the upper transverse support is of a rectangular plate structure, the upper fixing hole is 12 small holes uniformly distributed on the upper transverse support, the lower transverse support is of a rectangular plate structure, the lower fixing hole is 12 small holes uniformly distributed on the lower transverse support and corresponds to the upper fixing holes one by one, the electromagnetic device is formed by 12 copper enameled coils and 12 soft iron cylinders, and the electromagnetic device is positioned inside the rectangular box body of the vertical support to form an electromagnet. The magnetic force can be controlled at any time, the magnetic beads can be controlled, the batch washing effect is realized, and the working efficiency can be improved.
Description
Technical Field
The invention belongs to the technical field of molecular biology, and mainly relates to an electromagnetic force support of a nucleic acid extraction centrifugal tube.
Background
The magnetic bead method for extracting nucleic acid can be generally divided into four steps: cracking, combining, washing and eluting. The magnetic bead method for extracting nucleic acid is to crack sample cells by using a cell lysis solution, nucleic acid molecules dissociated from the cells are specifically adsorbed to the surfaces of the magnetic beads to form magnetic bead-nucleic acid polymers, and impurities such as protein and the like are not adsorbed and remain in the solution. After reacting for a certain time, under the action of a magnetic field, separating the magnetic bead-nucleic acid polymer from the liquid, recovering the magnetic bead-nucleic acid polymer, and then eluting with an eluent to obtain pure nucleic acid. The magnetic bead method does not need to add various reagents and centrifugation, and is an important direction for developing a future nucleic acid purification method. The magnetic bead method needs to use a PCR tube magnetic frame, the existing PCR tube magnetic frame is of a structure that a permanent magnet block and a support are integrated, although the magnetic bead-nucleic acid polymer can be rapidly gathered, in the washing and elution process, the magnetic bead-nucleic acid polymer cannot be dispersed due to the permanent magnetic effect of a magnet, and the PCR tubes on the PCR tube magnetic frame need to be taken down one by one and shaken to enable the magnetic bead-nucleic acid polymer to obtain washing liquid or eluent, which is very inconvenient. Therefore, a magnetic frame of a PCR tube capable of regulating and controlling a magnetic field is very needed at present.
Disclosure of Invention
The invention aims to provide an electromagnetic force bracket of a nucleic acid extraction centrifugal tube, which can effectively, quickly and safely complete a nucleic acid extraction test.
Therefore, the technical scheme adopted by the invention is that the invention provides an electromagnetic force support of a nucleic acid extraction centrifugal tube, which comprises a vertical support, an upper transverse support, an upper fixing hole, a lower transverse support, a lower fixing hole, an electromagnetic device, a switch and a power socket, wherein the vertical support is a rectangular box body formed by plates, 6 small holes are uniformly distributed on two sides below the vertical support, the total number of the small holes is 12, the upper transverse support is of a rectangular plate structure, the upper fixing hole is formed by uniformly distributing 12 small holes on the upper transverse support, the lower transverse support is of a rectangular plate structure, the lower fixing hole is formed by uniformly distributing 12 small holes on the lower transverse support and is in one-to-one correspondence with the upper fixing holes, the vertical support, the upper transverse support and the lower transverse support are formed by fixing screws, the 12 small holes on two sides of the rectangular box body of the vertical support and the 12 lower fixing holes are in one-to-one correspondence with each other on a vertical line, electromagnetic means constitute by 12 enamelled coils and 12 soft iron cylinder that copper was made, it is located the inside electro-magnet that constitutes of rectangle box of erecting the support, 12 soft iron cylinder are worn out from the 12 aperture of the rectangle box both sides of erecting the support respectively, electromagnetic means's enamelled coil one end be connected with one end of switch, another one end of switch is connected with one end of 12V DC power supply socket, electromagnetic means's enamelled coil another is connected with another one of 12V DC power supply socket, switch and supply socket fix respectively on erecting the support. The outer side length of the vertical support is 132-136mm, the outer height is 28-32mm, the outer thickness is 27-31mm, the inner side length is 128-132mm, the inner height is 24-28mm, the inner thickness is 23-27mm, the diameter of the small hole of the vertical support is 11.5-13.5mm, the length of the upper transverse support is 128-132mm, the width is 108-112mm, the thickness is 4-6mm, the diameter of the upper fixing hole is 10.5-11mm, the length of the lower transverse support is 128-132mm, the width is 123-127mm, the thickness is 4-6mm, the diameter of the lower fixing hole is 4.8-5.0mm, the diameter of the soft iron cylinder is 11-13mm, and the height is 11-13 mm. The vertical support, the upper horizontal support and the lower horizontal support are made of aluminum alloy or medical plastic.
The principle of the invention is that the principle of the electromagnet is utilized, the existence of magnetic force can be controlled at any time, the control of magnetic beads is achieved, the batch washing effect is realized, and the working efficiency can be improved.
The technical scheme of the invention has the following advantages: the novel solar water heater has the advantages of small volume, light weight, simple structure, low price, safe and reliable use, great saving of manpower and material resources, environmental protection and capability of meeting market demands.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and claims hereof as well as the appended drawings.
The technical solution of the present invention is further described in detail by the accompanying drawings and embodiments.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention.
FIG. 1 is a schematic structural diagram of the present invention.
In the figure, 1 stands vertically; 2, an upper transverse bracket; 3, fixing holes are formed; 4, a lower transverse bracket; 5, lower fixing holes; 6 an electromagnetic device; 7, a switch; 8, a power socket; 9 soft iron cylinder.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
In the description of the present application, it is to be understood that the terms "intermediate," "top," "bottom," "inner," "outer," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the present application and simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed in a particular orientation, and be operated in a particular orientation, and thus should not be construed as limiting the present application. The terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.
In addition, unless expressly stated or limited otherwise, the terms "mounted," "connected," "secured," and the like are intended to be inclusive and mean, for example, that there may be a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.
In this application, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact of the first and second features, or may comprise contact of the first and second features not directly but through another feature in between. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
Referring to the figure, the invention provides an electromagnetic force support of a nucleic acid extraction centrifugal tube, which comprises a vertical support 1, an upper transverse support 2, an upper fixing hole 3, a lower transverse support 4, a lower fixing hole 5, an electromagnetic device 6, a switch 7 and a power socket 8, wherein the vertical support 1 is a rectangular box body formed by plates, 6 small holes are uniformly distributed on two sides below the vertical support, the total number of the small holes is 12, the upper transverse support 2 is of a rectangular plate structure, the upper fixing hole 3 is formed by uniformly distributing 12 small holes on the upper transverse support 2, the lower transverse support 4 is of a rectangular plate structure, the lower fixing hole 5 is formed by uniformly distributing 12 small holes on the lower transverse support 4 and corresponding to the upper fixing holes one by one, the vertical support 1, the upper transverse support 2 and the lower transverse support 4 are formed by fixing screws, and the 12 upper fixing holes 3, the 12 small holes on two sides of the rectangular box body of the vertical support 1 and the 12 lower fixing holes 5 are respectively on a vertical line Last one-to-one, electromagnetic means 6 constitute by 12 enamelled coil and 12 soft iron cylinder 9 of copper, it is located the inside electro-magnet that constitutes of rectangle box of erecting support 1, 12 soft iron cylinder 9 wear out from the 12 aperture of the rectangle box both sides of erecting support 1 respectively, electromagnetic means 6's enamelled coil one end be connected with one end of switch 7, another end of switch 7 is connected with one end of 12V DC power supply socket 8, another end of electromagnetic means 6's enamelled coil is connected with another end of 12V DC power supply socket 8, switch 7 and power supply socket 8 are fixed respectively on erecting support 1. The outer side length of the vertical support 1 is 132-136mm, the outer height is 28-32mm, the outer thickness is 27-31mm, the inner side length is 128-132mm, the inner height is 24-28mm, the inner thickness is 23-27mm, the diameter of the small hole of the vertical support 1 is 11.5-13.5mm, the length of the upper transverse support 2 is 128-132mm, the width is 108-112mm, and the thickness is 4-6mm, the diameter of the upper fixing hole 3 is 10.5-11mm, the length of the lower transverse support 4 is 128-132mm, the width is 123-127mm, and the thickness is 4-6mm, the diameter of the lower fixing hole 5 is 4.8-5.0mm, the diameter of the soft iron cylinder 9 is 11-13mm, and the height is 11-13 mm. The vertical support 1, the upper horizontal support 2 and the lower horizontal support 4 are made of aluminum alloy or medical plastic.
When the invention is used, firstly, an operator inserts a 12V direct current power supply into a power socket 8, then samples of nucleic acid to be extracted are respectively added into centrifuge tubes, the centrifuge tubes are respectively inserted into an upper fixing hole 3 of an upper horizontal bracket 2 of the invention, the centrifuge tubes are fixed in an upper fixing hole 3 and a lower fixing hole 5, then lysate is respectively added, after 5-10 minutes, magnetic beads are respectively added, after 1-2 minutes, the invention is held by hands and slightly shaken or put on an oscillator to slightly shake the magnetic beads and the nucleic acid polymer to be fully combined to form the magnetic bead-nucleic acid polymer, at the moment, a switch 7 is opened, an electromagnetic device 6 generates magnetic force through a soft iron cylinder 9, the magnetic bead-nucleic acid polymer is adsorbed on the side surface of the centrifuge tube, at the moment, liquid containing impurities such as protein and the like is absorbed by a sample adding device, then washing liquid is added, the switch 7 is closed, the electromagnetic device 6 disappears through the magnetic force generated by the soft iron cylinder 9, the invention is lightly shaken by hands or placed on an oscillator to enable the nucleic acid sample liquid to be fully washed and the magnetic beads to be fully combined with the nucleic acid polymer to form the magnetic bead-nucleic acid polymer, the switch 7 is opened again after 2 to 3 minutes, the electromagnetic device 6 generates the magnetic force through the soft iron cylinder 9 to enable the magnetic bead-nucleic acid polymer to be adsorbed on the side surface of the centrifuge tube, the liquid containing the washing liquid is absorbed by a sample adding device, then the eluent is added, the switch 7 is closed, the electromagnetic device 6 disappears through the magnetic force generated by the soft iron cylinder 9, the invention is lightly shaken by hands or placed on the oscillator to enable the nucleic acid sample liquid to be fully washed and the magnetic beads to be fully eluted with the nucleic acid polymer for 2 to 3 minutes to enable the magnetic beads to be fully separated from the nucleic acid polymer, and the switch 7 is turned on again, the electromagnetic device 6 generates magnetic force through the soft iron cylinder 9, the magnetic beads are adsorbed on the side surface of the centrifugal tube, the liquid containing the eluent is absorbed by the sample injector, and the eluent contains the nucleic acid in the sample and is used for nucleic acid detection.
The invention can control the existence of magnetic force at any time by utilizing the principle of the electromagnet, achieves the control of magnetic beads, realizes the function of batch washing, has small volume, light weight, simple structure, low price and safe and reliable use, greatly saves manpower and material resources, is green and environment-friendly, and meets the market demand.
It should be noted that the above embodiments are all preferred embodiments, relevant functional components may be replaced by other components, and the related units and modules are not necessarily required by the present application. The embodiments in the present specification are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The electromagnetic force bracket for the nucleic acid extraction centrifugal tube provided by the application is described in detail above, and the principle and the implementation mode of the application are explained by applying specific examples, and the description of the examples is only used for helping to understand the method and the core idea of the application; meanwhile, for a person skilled in the art, according to the idea of the present application, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present application.
Claims (3)
1. The utility model provides a nucleic acid extraction centrifuging tube electromagnetic force support which characterized in that: the electromagnetic device comprises a vertical support, an upper transverse support, an upper fixing hole, a lower transverse support, a lower fixing hole, an electromagnetic device, a switch and a power socket, wherein the vertical support is a rectangular box body formed by plates, two sides below the vertical support are respectively provided with 6 small holes which are uniformly distributed, the total number of the small holes is 12, the upper transverse support is of a rectangular plate structure, the upper fixing hole is formed by uniformly distributing 12 small holes on the upper transverse support, the lower transverse support is of a rectangular plate structure, the lower fixing hole is formed by uniformly distributing 12 small holes on the lower transverse support and corresponds to the upper fixing hole one by one, the vertical support, the upper transverse support and the lower transverse support are fixed by screws for forming, the 12 small holes on two sides of the rectangular box body of the vertical support and the 12 lower fixing holes on two sides of the rectangular box body of the vertical support are respectively in one-to-one correspondence on a vertical line, the electromagnetic device is formed by 12 copper enameled coils and 12 soft iron cylinders, the electromagnetic device is positioned in a rectangular box body of the vertical support to form an electromagnet, 12 soft iron cylinders respectively penetrate out of 12 small holes in two sides of the rectangular box body of the vertical support, one end of an enamelled coil of the electromagnetic device is connected with one end of a switch, the other end of the switch is connected with one end of a 12V direct-current power socket, the other end of the enamelled coil of the electromagnetic device is connected with the other end of the 12V direct-current power socket, and the switch and the power socket are respectively fixed on the vertical support.
2. The electromagnetic force bracket of the nucleic acid extraction centrifuge tube of claim 1, which is characterized in that: the outer side length of the vertical support is 132-136mm, the outer height is 28-32mm, the outer thickness is 27-31mm, the inner side length is 128-132mm, the inner height is 24-28mm, the inner thickness is 23-27mm, the diameter of the small hole of the vertical support is 11.5-13.5mm, the length of the upper transverse support is 128-132mm, the width is 108-112mm, the thickness is 4-6mm, the diameter of the upper fixing hole is 10.5-11mm, the length of the lower transverse support is 128-132mm, the width is 123-127mm, the thickness is 4-6mm, the diameter of the lower fixing hole is 4.8-5.0mm, the diameter of the soft iron cylinder is 11-13mm, and the height is 11-13 mm.
3. The electromagnetic force bracket of the nucleic acid extraction centrifuge tube of claim 1, which is characterized in that: the vertical support, the upper horizontal support and the lower horizontal support are made of aluminum alloy or medical plastic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010082445.4A CN111013689A (en) | 2020-02-07 | 2020-02-07 | Electromagnetic force support of nucleic acid extraction centrifuge tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010082445.4A CN111013689A (en) | 2020-02-07 | 2020-02-07 | Electromagnetic force support of nucleic acid extraction centrifuge tube |
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| CN111013689A true CN111013689A (en) | 2020-04-17 |
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| CN202010082445.4A Pending CN111013689A (en) | 2020-02-07 | 2020-02-07 | Electromagnetic force support of nucleic acid extraction centrifuge tube |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112093233A (en) * | 2020-09-18 | 2020-12-18 | 南京医科大学 | Graphene targeted drug carrier, preparation method and application thereof, and transport box |
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2020
- 2020-02-07 CN CN202010082445.4A patent/CN111013689A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112093233A (en) * | 2020-09-18 | 2020-12-18 | 南京医科大学 | Graphene targeted drug carrier, preparation method and application thereof, and transport box |
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Application publication date: 20200417 |