CN211886975U

CN211886975U - Electromagnetic force support of nucleic acid extraction centrifuge tube

Info

Publication number: CN211886975U
Application number: CN202020155265.XU
Authority: CN
Inventors: 汪明会; 闫换新; 刘伟; 俞青
Original assignee: Beijing Yiming Shengtai Technology Co ltd; Beijing Yiwave Intelligent Technology Co ltd
Current assignee: Beijing Yiming Shengtai Technology Co ltd; Beijing Yiwave Intelligent Technology Co ltd
Priority date: 2020-02-07
Filing date: 2020-02-07
Publication date: 2020-11-10
Anticipated expiration: 2030-02-07

Abstract

The utility model discloses a nucleic acid draws centrifuging tube electromagnetic force support, including erecting support, last horizontal stand, going up the fixed orifices, horizontal stand, lower fixed orifices, electromagnetic means, switch and supply socket down, perpendicular support be a rectangle box of compriseing panel, its below both sides respectively have evenly distributed's 6 apertures, 12 apertures in total, last horizontal stand be rectangle plate structure, last fixed orifices be evenly distributed's 12 apertures on last horizontal stand, lower horizontal stand be rectangle plate structure, lower fixed orifices be evenly distributed 12 apertures on horizontal stand down to with last fixed orifices one-to-one, electromagnetic means constitute by 12 enamelled wire circle and 12 soft iron cylinder of copper, it is located the inside electro-magnet that constitutes of box of erecting the support. The magnetic force can be controlled at any time, the magnetic beads can be controlled, the batch washing effect is realized, and the working efficiency can be improved.

Description

Electromagnetic force support of nucleic acid extraction centrifuge tube

Technical Field

The utility model belongs to the technical field of molecular biology, mainly relate to a nucleic acid extraction centrifuging tube electromagnetic force support.

Background

The magnetic bead method for extracting nucleic acid can be generally divided into four steps: cracking, combining, washing and eluting. The magnetic bead method for extracting nucleic acid is to crack sample cells by using a cell lysis solution, nucleic acid molecules dissociated from the cells are specifically adsorbed to the surfaces of the magnetic beads to form magnetic bead-nucleic acid polymers, and impurities such as protein and the like are not adsorbed and remain in the solution. After reacting for a certain time, under the action of a magnetic field, separating the magnetic bead-nucleic acid polymer from the liquid, recovering the magnetic bead-nucleic acid polymer, and then eluting with an eluent to obtain pure nucleic acid. The magnetic bead method does not need to add various reagents and centrifugation, and is an important direction for developing a future nucleic acid purification method. The magnetic bead method needs to use a PCR tube magnetic frame, the existing PCR tube magnetic frame is of a structure that a permanent magnet block and a support are integrated, although the magnetic bead-nucleic acid polymer can be rapidly gathered, in the washing and elution process, the magnetic bead-nucleic acid polymer cannot be dispersed due to the permanent magnetic effect of a magnet, and the PCR tubes on the PCR tube magnetic frame need to be taken down one by one and shaken to enable the magnetic bead-nucleic acid polymer to obtain washing liquid or eluent, which is very inconvenient. Therefore, a magnetic frame of a PCR tube capable of regulating and controlling a magnetic field is very needed at present.

Disclosure of Invention

An object of the utility model is to provide a nucleic acid extraction centrifuging tube electromagnetic force support can accomplish the nucleic acid extraction test effectively, fast and safely, the utility model discloses used the electro-magnet, can control the effect that whether the magnetic field goes on in batches when realizing the washing to the effect of magnetic bead as required, can also improve work efficiency.

Therefore, the technical scheme is that the utility model provides a centrifugal tube electromagnetic force support for nucleic acid extraction, which comprises a vertical support, an upper horizontal support, an upper fixing hole, a lower horizontal support, a lower fixing hole, an electromagnetic device, a switch and a power socket, wherein the vertical support is a rectangular box body formed by plates, the two sides below the vertical support are respectively provided with 6 small holes which are uniformly distributed, the total number of the small holes is 12, the upper horizontal support is of a rectangular plate structure, the upper fixing hole is formed by uniformly distributing 12 small holes on the upper horizontal support, the lower horizontal support is of a rectangular plate structure, the lower fixing hole is formed by uniformly distributing 12 small holes on the lower horizontal support and is in one-to-one correspondence with the upper fixing hole, the vertical support, the upper horizontal support and the lower horizontal support are formed by screw fixation, the 12 small holes on the two sides of the rectangular box body of the upper fixing hole and the vertical support and the 12 lower fixing holes are respectively in one-to-one correspondence with each other on a vertical line, electromagnetic means constitute by 12 enamelled coils and 12 soft iron cylinder that copper was made, it is located the inside electro-magnet that constitutes of rectangle box of erecting the support, 12 soft iron cylinder are worn out from the 12 aperture of the rectangle box both sides of erecting the support respectively, electromagnetic means's enamelled coil one end be connected with one end of switch, another one end of switch is connected with one end of 12V DC power supply socket, electromagnetic means's enamelled coil another is connected with another one of 12V DC power supply socket, switch and supply socket fix respectively on erecting the support. The outer side length of the vertical support is 132-136mm, the outer height is 28-32mm, the outer thickness is 27-31mm, the inner side length is 128-132mm, the inner height is 24-28mm, the inner thickness is 23-27mm, the diameter of the small hole of the vertical support is 11.5-13.5mm, the length of the upper transverse support is 128-132mm, the width is 108-112mm, the thickness is 4-6mm, the diameter of the upper fixing hole is 10.5-11mm, the length of the lower transverse support is 128-132mm, the width is 123-127mm, the thickness is 4-6mm, the diameter of the lower fixing hole is 4.8-5.0mm, the diameter of the soft iron cylinder is 11-13mm, and the height is 11-13 mm. The vertical support, the upper horizontal support and the lower horizontal support are made of aluminum alloy or medical plastic.

The utility model discloses an utilize the principle of electro-magnet, can control magnetic force at any time and have or not, reach the control to the magnetic bead, effect that goes on in batches when realizing the washing can also improve work efficiency.

The utility model discloses technical scheme has following advantage: the novel solar water heater has the advantages of small volume, light weight, simple structure, low price, safe and reliable use, great saving of manpower and material resources, environmental protection and capability of meeting market demands.

Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by the practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and claims hereof as well as the appended drawings.

The technical solution of the present invention is further described in detail by the accompanying drawings and examples.

Drawings

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention.

Fig. 1 is a schematic structural diagram of the present invention.

In the figure, 1 stands vertically; 2, an upper transverse bracket; 3, fixing holes are formed; 4, a lower transverse bracket; 5, lower fixing holes; 6 an electromagnetic device; 7, a switch; 8, a power socket; 9 soft iron cylinder.

Detailed Description

In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the detailed description.

In the description of the present application, it is to be understood that the terms "intermediate," "top," "bottom," "inner," "outer," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the present application and simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed in a particular orientation, and be operated in a particular orientation, and thus should not be construed as limiting the present application. The terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.

In addition, unless expressly stated or limited otherwise, the terms "mounted," "connected," "secured," and the like are intended to be inclusive and mean, for example, that there may be a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.

In this application, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact of the first and second features, or may comprise contact of the first and second features not directly but through another feature in between. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

Referring to the drawings, the utility model provides a nucleic acid draws centrifuging tube electromagnetic force support, including perpendicular support 1, last horizontal support 2, go up fixed orifices 3, lower horizontal support 4, lower fixed orifices 5, electromagnetic means 6, switch 7 and supply socket 8, perpendicular support 1 be a rectangle box that comprises panel, its below both sides respectively have 6 apertures of evenly distributed, 12 apertures in total, last horizontal support 2 be rectangle plate structure, last fixed orifices 3 be 2 evenly distributed 12 apertures on last horizontal support, lower horizontal support 4 be rectangle plate structure, lower fixed orifices 5 evenly distributed 12 apertures on lower horizontal support 4 to with last fixed orifices one-to-one, perpendicular support 1, last horizontal support 2, lower horizontal support 4 be the screw fixation and the shaping, 12 last fixed orifices 3, Erect 12 apertures and 12 lower fixed orificess 5 of the rectangle box both sides of support 1 and be one-to-one on a perpendicular vertical line respectively, electromagnetic means 6 constitute by 12 copper enamelled coil and 12 soft iron cylinder 9, it is located the inside electro-magnet that constitutes of the rectangle box of erecting support 1, 12 soft iron cylinder 9 are worn out from the 12 apertures of the rectangle box both sides of erecting support 1 respectively, one end of the enamelled coil of electromagnetic means 6 be connected with one end of switch 7, another end of switch 7 is connected with one end of 12V DC power supply socket 8, another end of the enamelled coil of electromagnetic means 6 is connected with another end of 12V DC power supply socket 8, switch 7 and power supply socket 8 are fixed respectively on erecting support 1. The outer side length of the vertical support 1 is 132-136mm, the outer height is 28-32mm, the outer thickness is 27-31mm, the inner side length is 128-132mm, the inner height is 24-28mm, the inner thickness is 23-27mm, the diameter of the small hole of the vertical support 1 is 11.5-13.5mm, the length of the upper transverse support 2 is 128-132mm, the width is 108-112mm, and the thickness is 4-6mm, the diameter of the upper fixing hole 3 is 10.5-11mm, the length of the lower transverse support 4 is 128-132mm, the width is 123-127mm, and the thickness is 4-6mm, the diameter of the lower fixing hole 5 is 4.8-5.0mm, the diameter of the soft iron cylinder 9 is 11-13mm, and the height is 11-13 mm. The vertical support 1, the upper horizontal support 2 and the lower horizontal support 4 are made of aluminum alloy or medical plastic.

When the utility model is used, firstly, an operator inserts a 12V direct current power supply on a power socket 8, then a sample to be extracted nucleic acid is respectively added into a centrifuge tube, and the centrifuge tube is respectively inserted from an upper fixing hole 3 of an upper cross support 2 of the utility model, so that the centrifuge tube is fixed in the upper fixing hole 3 and a lower fixing hole 5, then lysate is respectively added, after 5-10 minutes, magnetic beads are respectively added, after 1-2 minutes, the utility model is held by hand and lightly shaken or put on an oscillator to make the magnetic beads and the nucleic acid polymer fully combined to form the magnetic beads-nucleic acid polymer, at the moment, a switch 7 is opened, an electromagnetic device 6 generates magnetic force through a soft iron cylinder 9, the magnetic beads-nucleic acid polymer is adsorbed on the side surface of the centrifuge tube, at the moment, the liquid containing impurities such as protein and the like is absorbed by a sample adding device, then add the washing liquid, close switch 7, electromagnetic means 6 disappears through the magnetic force that soft iron cylinder 9 produced, will the utility model discloses hold gently to rock or put on the oscillator gently to rock and make nucleic acid sample liquid fully wash and magnetic ball and nucleic acid polymer fully combine, form magnetic ball-nucleic acid polymer, open switch 7 again after 2-3 minutes, electromagnetic means 6 produces magnetic force through soft iron cylinder 9, adsorb magnetic ball-nucleic acid polymer in the side of centrifuging tube, reuse injector will contain the liquid of washing liquid and suck away, add the eluant, close switch 7, electromagnetic means 6 disappears through the magnetic force that soft iron cylinder 9 produced, will the utility model discloses hold gently to rock or put on the oscillator gently to rock and make nucleic acid sample liquid fully wash and magnetic ball and nucleic acid polymer fully elute 2-3 minutes, make magnetic ball and nucleic acid polymer fully separate, open switch 7 again, electromagnetic means 6 produces magnetic force through soft iron cylinder 9, adsorbs magnetic ball in the side of centrifuging tube, and the liquid that reuse application of sample ware will contain the eluant is siphoned away, and the eluant contains the nucleic acid in the sample this moment, is used for nucleic acid detection.

The utility model discloses an utilize the principle of electromagnet, can control magnetic force at any time and have or not, reach the control to the magnetic bead, effect that goes on in batches when realizing the washing, moreover small, light in weight, simple structure, low price and use safe and reliable have practiced thrift manpower and material resources greatly, and green accords with the market demand moreover.

It should be noted that the above embodiments are all preferred embodiments, relevant functional components may be replaced by other components, and the related units and modules are not necessarily required by the present application. The embodiments in the present specification are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.

The electromagnetic force bracket for the nucleic acid extraction centrifugal tube provided by the application is described in detail above, and the principle and the implementation mode of the application are explained by applying specific examples, and the description of the examples is only used for helping to understand the method and the core idea of the application; meanwhile, for a person skilled in the art, according to the idea of the present application, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present application.

Claims

1. The utility model provides a nucleic acid extraction centrifuging tube electromagnetic force support which characterized in that: the electromagnetic device comprises a vertical support, an upper transverse support, an upper fixing hole, a lower transverse support, a lower fixing hole, an electromagnetic device, a switch and a power socket, wherein the vertical support is a rectangular box body formed by plates, two sides below the vertical support are respectively provided with 6 small holes which are uniformly distributed, the total number of the small holes is 12, the upper transverse support is of a rectangular plate structure, the upper fixing hole is formed by uniformly distributing 12 small holes on the upper transverse support, the lower transverse support is of a rectangular plate structure, the lower fixing hole is formed by uniformly distributing 12 small holes on the lower transverse support and corresponds to the upper fixing hole one by one, the vertical support, the upper transverse support and the lower transverse support are fixed by screws for forming, the 12 small holes on two sides of the rectangular box body of the vertical support and the 12 lower fixing holes on two sides of the rectangular box body of the vertical support are respectively in one-to-one correspondence on a vertical line, the electromagnetic device is formed by 12 copper enameled coils and 12 soft iron cylinders, the electromagnetic device is positioned in a rectangular box body of the vertical support to form an electromagnet, 12 soft iron cylinders respectively penetrate out of 12 small holes in two sides of the rectangular box body of the vertical support, one end of an enamelled coil of the electromagnetic device is connected with one end of a switch, the other end of the switch is connected with one end of a 12V direct-current power socket, the other end of the enamelled coil of the electromagnetic device is connected with the other end of the 12V direct-current power socket, and the switch and the power socket are respectively fixed on the vertical support.

2. The electromagnetic force bracket of the nucleic acid extraction centrifuge tube of claim 1, which is characterized in that: the outer side length of the vertical support is 132-136mm, the outer height is 28-32mm, the outer thickness is 27-31mm, the inner side length is 128-132mm, the inner height is 24-28mm, the inner thickness is 23-27mm, the diameter of the small hole of the vertical support is 11.5-13.5mm, the length of the upper transverse support is 128-132mm, the width is 108-112mm, the thickness is 4-6mm, the diameter of the upper fixing hole is 10.5-11mm, the length of the lower transverse support is 128-132mm, the width is 123-127mm, the thickness is 4-6mm, the diameter of the lower fixing hole is 4.8-5.0mm, the diameter of the soft iron cylinder is 11-13mm, and the height is 11-13 mm.

3. The electromagnetic force bracket of the nucleic acid extraction centrifuge tube of claim 1, which is characterized in that: the vertical support, the upper horizontal support and the lower horizontal support are made of aluminum alloy or medical plastic.