CN212833819U - Device for sorting cells by using magnetic beads - Google Patents
Device for sorting cells by using magnetic beads Download PDFInfo
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- CN212833819U CN212833819U CN202021485327.XU CN202021485327U CN212833819U CN 212833819 U CN212833819 U CN 212833819U CN 202021485327 U CN202021485327 U CN 202021485327U CN 212833819 U CN212833819 U CN 212833819U
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Abstract
The utility model discloses an utilize magnetic bead to select separately device of cell, including mount table, lid shroud plate, mixed funnel, sorting platform and rotating turret, the mount table is L type platform column structure, the both sides of mount table are all articulated to be installed the lid shroud plate, mount table bottom department integrated into one piece stretches out there is the cooperation board, be fixed with the mount of U type directly over the corresponding sorting platform of one side of mount table, mix funnel bottom department and peg graft and install the inserted sheet, mix funnel bottom department and be provided with the intercommunication box, intercommunication box bottom department is provided with the conduction pipe, buffer tank top department is provided with communicating pipe, and the middle-section department of communicating pipe is provided with the liquid pump, and the one end setting of keeping away from the buffer tank communicating pipe is in mixed funnel top department. This utilize device of magnetic bead sorting cell, reasonable in design can carry out fine fixed placing to the test tube, guarantees the stability of doing simultaneously, can carry out fine accepting, is fit for using widely to the cell liquid of selecting separately through the test tube.
Description
Technical Field
The utility model belongs to the technical field of bioengineering, concretely relates to utilize magnetic bead to select separately device of cell.
Background
Magnetic bead enrichment or separation is an emerging molecular and cell biology technology at the end of the last century, and the technology is widely applied to nucleic acid extraction, solid-phase cDNA library construction, protein or cell sorting or enrichment in molecular biology. The construction principle is that a specific ligand or an antibody is jointed on the surface of a naked magnetic bead particle by a biochemical technical means. The working principle is that magnetic bead suspension with a ligand (anti) body connected on the surface is mixed with target molecules to be selected or cell solution (suspension), the magnetic beads are tightly combined with the target molecules or cells through ligand-receptor binding reaction or antigen-antibody reaction, and then the magnetic bead-target complex is captured through magnetic force, so that the aim of enriching or purifying the target molecules and cells is achieved.
The existing device for sorting cells by utilizing magnetic beads is mainly used for carrying separated cell sap by manually holding a test tube, and the problems that partial cells are spilled due to hand shaking of a user and the like often occur.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an utilize device of magnetic bead separation cell to solve the problem that proposes among the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme: a device for sorting cells by using magnetic beads comprises an installation platform, a cover plate, a mixing funnel, a sorting platform and a rotating frame, the mounting table is of an L-shaped table structure, the two sides of the mounting table are both hinged with cover plates, a matching plate integrally formed at the bottom of the mounting table, a sorting table fixed at the middle section of the top of the matching plate corresponding to the extending end, a rotating frame and a buffer tank respectively arranged at two sides of the matching plate corresponding to the sorting table, a U-shaped fixing frame is fixed on one side of the mounting table corresponding to the right upper part of the sorting table, a mixing funnel is erected on the fixing frame, the bottom of the mixing funnel is provided with an inserting sheet in an inserting way, the bottom of the mixing funnel is provided with a communicating box, the bottom of the communicating box is provided with a communicating pipe, buffer solution tank top department is provided with communicating pipe, and the midsection department of communicating pipe is provided with the liquid pump, and the one end setting of keeping away from buffer solution tank communicating pipe is in mixing funnel top department.
Preferably, the sorting platform includes bearing platform, electro-magnet, conduction pipe, the bearing platform is fixed on the cooperation board, bearing platform top department is provided with oval-shaped electro-magnet, the one end and the intercommunication box intercommunication of conduction pipe, the conduction pipe is the U type and arranges in the bearing platform, the conduction pipe box is established at the electro-magnet outer fringe, and the conduction pipe keeps away from the one end of connecting the box and stretches out.
Preferably, the rotating frame comprises a fixing column, a rotating ring and a clamping structure, the rotating ring is sleeved on the outer edge of the fixing column at equal intervals, a plurality of groups of insertion holes for inserting the test tubes are formed in the rotating ring by taking the axis of the rotating ring as a central annular array, and the clamping structure is arranged at the insertion holes.
Preferably, the bottom of the fixed column extends into the mounting table, a fastening plate is sleeved at the position, close to the outer edge of the bottom, of the fixed column, and a fastening knob is arranged on the fastening plate.
Preferably, press from both sides tight structure and include spring, gleitbretter, guide bar and press from both sides tight piece, the chamber way has been seted up to the bilateral symmetry that corresponds spliced eye department in the swivel becket, and the inside slip of chamber way is provided with the gleitbretter, and one side of the inside gleitbretter of chamber way is provided with the spring, the one end that the spring was kept away from to the gleitbretter is fixed with the extension bar, and the one end of extension bar stretches into in the spliced eye and is fixed with and presss from both sides tight piece, it is fixed with the.
The utility model discloses a technological effect and advantage: this utilize device of magnetic bead sorting cell, sorting platform through the setting, the conduction pipe on the sorting platform, accept the platform, cooperation between the electro-magnet, can carry out fine sorting processing to the cell that has the magnetic bead, enable the cell absorption that has the magnetic bead and close, through the rotating turret that sets up, fixed column on the rotating turret, swivel becket and fastening knob and the setting of a plurality of groups plug-in hole, can carry out fine placing to the test tube, and avoid the rotating turret to rotate in the course of the work, and then carry out fine accepting to the cell liquid that flows, avoid artifical hand to take the test tube to accept the cell liquid, through the tight structure of clamp that sets up, press from both sides the spring in the tight structure, the gleitbretter, the cooperation between guide bar and the tight piece of clamp, can carry out fine centre gripping fixedly to the test tube, guarantee the stability of the test tube. This utilize device of magnetic bead sorting cell, reasonable in design can carry out fine fixed placing to the test tube, guarantees the stability of doing simultaneously, can carry out fine accepting, is fit for using widely to the cell liquid of selecting separately through the test tube.
Drawings
Fig. 1 is a schematic structural view of the present invention;
fig. 2 is a schematic structural view of the mounting table of the present invention;
FIG. 3 is a cross-sectional view of the sorting deck of FIG. 2 according to the present invention;
FIG. 4 is a cross-sectional view of the rotating ring B-B of FIG. 2 according to the present invention;
fig. 5 is an enlarged view of the present invention at C in fig. 2;
fig. 6 is an enlarged view of the point D in fig. 4 according to the present invention.
In the figure: the device comprises a mounting table 1, a cover covering plate 2, a mixing funnel 3, a communication table 31, an insertion piece 32, a fixing frame 4, a liquid pump 5, a buffer liquid box 6, a sorting table 7, a conduction pipe 8, a bearing table 9, an electromagnet 10, a rotating frame 11, a fixing column 12, a rotating ring 13, a fastening knob 14, a clamping structure 15, a spring 16, a sliding sheet 17, a guide rod 18 and a clamping piece 19.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
The utility model provides a device for sorting cells by utilizing magnetic beads, as shown in figures 1-6, which comprises an installation platform 1, a cover plate 2, a mixing funnel 3, a sorting platform 7 and a rotating frame 11, wherein the installation platform 1 is of an L-shaped platform-shaped structure, the two sides of the installation platform 1 are both hinged with the cover plate 2, the cover plate 2 can cover the device integrally after working, thereby protecting the device well, a matching plate is extended at the bottom of the installation platform 1 in an integrated manner, the sorting platform 7 is fixed at the middle section of the top of the matching plate corresponding to an extending end, the rotating frame 11 and a buffer tank 6 are respectively arranged at the two sides of the matching plate corresponding to the sorting platform 7, a U-shaped fixing frame 4 is fixed right above the sorting platform 7 at one side of the installation platform 1, the mixing funnel 3 is arranged on the fixing frame 4, a splicing piece 32 is spliced and installed at the bottom of the mixing funnel 3, mix 3 bottoms departments of funnel and be provided with the intercommunication box 31, intercommunication box 31 bottoms department is provided with the conduction pipe 8, can install the separation pump at the midsection of conduction pipe 8 during the concrete implementation mode, 6 tops departments of buffer tank are provided with communicating pipe, and the midsection of communicating pipe is provided with liquid pump 5, and the one end setting of keeping away from buffer tank 6 communicating pipe is in 3 tops departments of mixing funnel.
It is specific, sorting table 7 includes bearing platform 9, electro-magnet 10, conduction pipe 8, bearing platform 9 is fixed on the cooperation board, bearing platform 9 top department is provided with oval-shaped electro-magnet 10, the one end and the intercommunication box 31 intercommunication of conduction pipe 8, conduction pipe 8 is the U type in bearing platform 9 and arranges, 8 covers of conduction pipe are established at electro-magnet 10 outer fringe, and the one end that conduction pipe 8 kept away from the connection box 31 stretches out, the end that stretches out of conduction pipe 8 sets up in the top department of test tube, and then conveniently carries out fine accepting at the cell liquid of efflux, can install little liquid funnel that connects on the test tube at the accepting in-process.
Specifically, the rotating frame 11 includes a fixed column 12, a rotating ring 13 and a clamping structure 15, the rotating frame 11 can well fix a plurality of groups of test tubes, the rotating ring 13 is sleeved on the outer edge of the fixed column 12 at equal intervals, a plurality of groups of insertion holes for insertion of the test tubes are arranged on the rotating ring 13 in an annular array by taking the axis of the rotating ring 13 as the center, and the clamping structure 15 is arranged at the insertion holes.
Specifically, the bottom of the fixed column 12 extends into the mounting table 1, a fastening plate is sleeved on the position, close to the outer edge of the bottom, of the fixed column 12, and a fastening knob 14 is arranged on the fastening plate.
It is specific, press from both sides tight structure 15 and include spring 16, gleitbretter 17, guide bar 18 and press from both sides tight piece 19, it can carry out fine centre gripping to the test tube fixedly to press from both sides tight structure 15, guarantees the stability of the test tube of during operation, the chamber way has been seted up to the bilateral symmetry that corresponds plug hole department in the swivel becket 13, and the inside slip of chamber way is provided with gleitbretter 17, and one side of the inside gleitbretter 17 of chamber way is provided with spring 16, the one end that spring 16 was kept away from to gleitbretter 17 is fixed with the extension bar, and the one end of extension bar stretches into in the plug hole and is fixed with and presss from both sides tight piece.
The working principle is as follows: the device for sorting cells by utilizing the magnetic beads is characterized in that when cells need to be sorted, the magnetic beads and samples are led into a mixing funnel 3, a certain amount of buffer solution is pumped into the mixing funnel 3 through a liquid pump 5, the buffer solution is stirred and mixed through a stirring rod, after the cells are stirred, a power supply is started, an electromagnet 10 is electrified through the power supply to generate a magnetic field, an insertion sheet 32 is pulled out at the moment, the mixed solution in the mixing funnel 3 passes through the magnetic field, the cells with the magnetic beads are adsorbed on the tube wall of a conduction tube 8, the rest cell solution flows into a test tube of a rotating frame 11 below the cell wall, the rotating frame 11 is rotated after the mixed solution is separated, a new test tube is rotated to the position below the extending end of the conduction tube 8, the liquid pump 5 is started, the buffer solution enters the mixing funnel 3, the power is cut off, and the cell solution with the magnetic beads flows into the new test tube, in the test tube inserting process, the test tube can be well clamped and fixed through the spring 16 and the clamping piece 19, and the device can be covered through the covering plate 2 after the use is finished. This utilize device of magnetic bead sorting cell, reasonable in design can carry out fine fixed placing to the test tube, guarantees the stability of doing simultaneously, can carry out fine accepting, is fit for using widely to the cell liquid of selecting separately through the test tube.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.
Claims (5)
1. The utility model provides an utilize device of magnetic bead sorting cell, includes mount table (1), cover apron (2), mixing funnel (3), sorting platform (7) and rotating turret (11), its characterized in that: the mounting table (1) is of an L-shaped table-shaped structure, cover plates (2) are hinged to two sides of the mounting table (1), a matching plate extends out of the bottom of the mounting table (1) in an integrated forming mode, a sorting table (7) is fixed to the matching plate at the middle position of the top of the extending end corresponding to the matching plate, a rotating frame (11) and a buffer liquid box (6) are arranged on the matching plate respectively at two sides corresponding to the sorting table (7), a U-shaped fixing frame (4) is fixed to one side of the mounting table (1) right above the sorting table (7) corresponding to the matching plate, a mixing funnel (3) is erected on the fixing frame (4), an inserting piece (32) is installed at the bottom of the mixing funnel (3) in an inserting mode, a communicating box (31) is arranged at the bottom of the mixing funnel (3), a communicating pipe (8) is arranged at the bottom of the communicating box (31), and a communicating pipe is arranged at, the middle section of the communicating pipe is provided with a liquid pump (5), and one end of the communicating pipe, which is far away from the buffer tank (6), is arranged at the top of the mixing funnel (3).
2. The apparatus of claim 1, wherein the apparatus further comprises: sorting platform (7) are including bearing platform (9), electro-magnet (10), conduction pipe (8), bearing platform (9) are fixed on the cooperation board, bearing platform (9) top department is provided with oval-shaped electro-magnet (10), the one end and the intercommunication box (31) intercommunication of conduction pipe (8), conduction pipe (8) are the U type in bearing platform (9) and arrange, conduction pipe (8) cover is established at electro-magnet (10) outer fringe, and the one end that conduction pipe (8) kept away from intercommunication box (31) stretches out.
3. The apparatus of claim 1, wherein the apparatus further comprises: the rotating frame (11) comprises a fixed column (12), a rotating ring (13) and a clamping structure (15), the rotating ring (13) is sleeved on the outer edge of the fixed column (12) at equal intervals, a plurality of groups of insertion holes for insertion of test tubes are formed in the rotating ring (13) by taking the axis of the rotating ring (13) as a central annular array, and the clamping structure (15) is arranged at the insertion holes.
4. A device for sorting cells using magnetic beads according to claim 3, wherein: the bottom of the fixing column (12) extends into the mounting table (1), a fastening plate is sleeved on the position, close to the bottom, of the fixing column (12), and a fastening knob (14) is arranged on the fastening plate.
5. A device for sorting cells using magnetic beads according to claim 3, wherein: press from both sides tight structure (15) and include spring (16), gleitbretter (17), guide bar (18) and press from both sides tight piece (19), the chamber way has been seted up to the bilateral symmetry that corresponds socket hole department in swivel becket (13), and the inside slip sheet (17) that slides of chamber way is provided with, and one side of the inside slip sheet (17) of chamber way is provided with spring (16), the one end that spring (16) were kept away from in gleitbretter (17) is fixed with the extension bar, and the one end of extension bar stretches into in the socket hole and is fixed with and presss from both sides tight piece (19), it is fixed with guide bar (18) to press from both sides a side symmetry that.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021485327.XU CN212833819U (en) | 2020-07-24 | 2020-07-24 | Device for sorting cells by using magnetic beads |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021485327.XU CN212833819U (en) | 2020-07-24 | 2020-07-24 | Device for sorting cells by using magnetic beads |
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| Publication Number | Publication Date |
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| CN212833819U true CN212833819U (en) | 2021-03-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202021485327.XU Active CN212833819U (en) | 2020-07-24 | 2020-07-24 | Device for sorting cells by using magnetic beads |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114164103A (en) * | 2021-12-02 | 2022-03-11 | 南京大学 | Semi-automatic biological magnetic separation method |
| CN118064269A (en) * | 2024-04-18 | 2024-05-24 | 苏州欣协生物科技有限公司 | Full-automatic closed cell preparation all-in-one |
| WO2024159554A1 (en) * | 2023-02-02 | 2024-08-08 | 中国人民解放军空军军医大学 | Magnetic bead cell sorting device for vitiligo |
-
2020
- 2020-07-24 CN CN202021485327.XU patent/CN212833819U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114164103A (en) * | 2021-12-02 | 2022-03-11 | 南京大学 | Semi-automatic biological magnetic separation method |
| WO2024159554A1 (en) * | 2023-02-02 | 2024-08-08 | 中国人民解放军空军军医大学 | Magnetic bead cell sorting device for vitiligo |
| CN118064269A (en) * | 2024-04-18 | 2024-05-24 | 苏州欣协生物科技有限公司 | Full-automatic closed cell preparation all-in-one |
| CN118064269B (en) * | 2024-04-18 | 2024-07-05 | 苏州欣协生物科技有限公司 | Full-automatic closed cell preparation all-in-one |
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