CN211921509U - Device and kit for extracting magnetic bead nucleic acid - Google Patents
Device and kit for extracting magnetic bead nucleic acid Download PDFInfo
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- CN211921509U CN211921509U CN202020084593.5U CN202020084593U CN211921509U CN 211921509 U CN211921509 U CN 211921509U CN 202020084593 U CN202020084593 U CN 202020084593U CN 211921509 U CN211921509 U CN 211921509U
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Abstract
The utility model relates to a device and kit for magnetic bead nucleic acid draws, including shelf, magnetic sheet and reaction tube detachably install in on the shelf, the bottom of reaction tube is provided with can dismantle the part, will dismantle the part and dismantle the back, the bottom of reaction tube becomes open. The utility model discloses a device makes in the nucleic acid extraction process, and the magnetic bead can conveniently remove the supernatant when adsorbing, only needs to dismantle the back with the detachable part of reaction tube bottom portion, and the supernatant can flow through the opening of bottom, and the magnetic bead that has combined with nucleic acid still adsorbs on the pipe wall, need not to invert whole device in order to get rid of the supernatant.
Description
Technical Field
The utility model belongs to the molecular biology experiment field, more particularly, relate to a device and kit for magnetic bead nucleic acid extraction.
Background
In recent years, the use of nano magnetic beads is more and more popular, and the fields are wider and wider, such as cell sorting, protein fixation, and nucleic acid purification and sorting. The magnetic beads are formed into superparamagnetic polymer nano particles by a chemical synthesis method, and the particles can realize different functional applications through various chemical modifications. The magnetic beads for nucleic acid purification are prepared by lysing cells, specifically adsorbing the liberated nucleic acid molecules onto the surface of magnetic particles, and simultaneously leaving impurities such as proteins in the solution. Then under the action of magnetic field, the magnetic particles are separated from the liquid, and the pure nucleic acid can be obtained by discarding the liquid and then eluting. Compared with other traditional methods for extracting nucleic acid, the method for extracting nucleic acid by using magnetic beads has incomparable unique advantages. Mainly shows the high efficiency, high specificity and high stability of extracting nucleic acid by magnetic beads.
Traditional magnetic bead nucleic acid extraction kits often only provide magnetic beads and extraction reagents, and users have their own EP tubes and magnetic racks. During the use, the user adds the lysate to the EP pipe earlier, add biological sample again, the schizolysis cell, then add the well-dispersed magnetic bead and mix in the EP pipe, place the EP pipe on the magnetic frame so that the magnetic bead adsorbs on the wall that the EP pipe is pressed close to the magnetic sheet, then pour the supernatant from the EP pipe mouth of pipe, then wash many times, remove the magnetic sheet earlier when washing each time, add the mixing of washing buffer solution and make the magnetic bead disperse, then install the magnetic sheet so that the magnetic bead adsorbs on the wall that the EP pipe is pressed close to the magnetic sheet, pour the supernatant from the EP pipe mouth of pipe, after washing many times repeatedly, elute with the eluant.
There is a great inconvenience in the above operations, that is, each time the supernatant is poured out from the nozzle of the EP tube, the EP tube needs to be kept stably attached to the magnetic plate and cannot be rotated. The difficulty is not great if only one sample is handled, but there is a problem if a plurality of samples are handled, because these samples must be handled together and any one sample cannot be moved at will. On the other hand, the dumping operation is easy to cause the EP pipe to generate dislocation, and on the other hand, the aperture ratio of the waste liquid cylinder is required to be larger, which brings inconvenience to operators.
Therefore, a new magnetic bead nucleic acid extraction kit is needed.
Disclosure of Invention
In order to solve the above problems, the utility model provides a device for extracting magnetic bead nucleic acid, which comprises a frame, a magnetic plate and a reaction tube, wherein the magnetic plate and the reaction tube are detachably arranged on the frame,
the frame comprises a base, a first supporting surface and a second supporting surface, wherein the first supporting surface is positioned above the second supporting surface and fixedly connected with the second supporting surface through a support column, the second supporting surface is connected with the base through a support column, the first supporting surface is provided with a reaction tube through hole through which a reaction tube can pass and a magnetic plate through hole through which a magnetic plate can pass, the reaction tube through hole is close to the magnetic plate through hole, the reaction tube through hole is circular, the second supporting surface is provided with a second reaction tube through hole corresponding to the reaction tube through hole of the first supporting surface and a second magnetic plate through hole corresponding to the magnetic plate through hole, and the diameter of the second reaction tube through hole is smaller than that of the reaction tube through hole;
the reaction tube is of a cylindrical structure with an opening at the top, the upper part of the reaction tube is cylindrical, the lower part of the reaction tube is conical, the diameter of the cross section of the cylindrical part of the reaction tube is equal to the maximum diameter of the cross section of the conical part, and the diameter of the cross section of the cylindrical part of the reaction tube is larger than the diameter of the through hole of the second reaction tube;
the bottom of the reaction tube is provided with a detachable part, and after the detachable part is detached, the bottom of the reaction tube is opened.
The utility model discloses a device makes in the nucleic acid extraction process, and the magnetic bead can conveniently remove the supernatant when adsorbing, only needs to dismantle the back with the detachable part of reaction tube bottom portion, and the supernatant can flow through the opening of bottom, and the magnetic bead that has combined with nucleic acid still adsorbs on the pipe wall, need not to invert whole device in order to get rid of the supernatant.
In a preferred embodiment, a protrusion is arranged on the region of the reaction tube through hole far away from the magnetic plate through hole, and a convex part corresponding to the protrusion is arranged on the outer wall of the reaction tube and can be clamped into the protrusion. The reaction tube is easy to rotate in the using process, so that the part adsorbing the magnetic beads is rotated away from the magnetic plate, and the magnetic beads and the nucleic acids carried on the magnetic beads enter the supernatant and are lost. The design in this scheme can prevent the rotation of reaction tube in the use.
In one embodiment, the bottom of the reaction tube and the removable member are configured to plug the hole plug structure. The scheme has simple structure and low product cost.
In a specific embodiment, the bottom of the reaction tube is provided with an external thread, and the detachable part is screwed to the bottom of the reaction tube. The detachable component is in threaded connection with the reaction tube, so that the operation is simple and convenient, and the effect is better by matching with the design of the protrusions and the convex parts on the detachable component.
In a preferred embodiment, the shelf is arranged such that the distance between the base and the second support surface is adjustable. The distance between the base and the second supporting surface can be adjusted according to specific conditions, so that a proper waste liquid container can be placed at the bottom of the reaction tube.
In a preferred embodiment, the reaction tube is further provided with a lid.
The invention also provides a kit for extracting magnetic bead nucleic acid, which comprises the device and a reagent for extracting magnetic bead nucleic acid.
Drawings
Fig. 1 is a side view of an embodiment of the present invention;
FIG. 2 is a schematic view of a first support surface according to an embodiment of the present invention;
FIG. 3 is a schematic view of a second support surface according to an embodiment of the present invention;
FIG. 4 is a schematic view of a reaction tube according to an embodiment of the present invention;
FIG. 5 is a schematic view of a reaction tube according to an embodiment of the present invention;
fig. 6 is a schematic view of a shelf according to an embodiment of the present invention.
Wherein the reference numerals denote the following: 1. a frame 11, a first support surface 111, a magnetic plate through hole 112, a reaction tube through hole 12, a second support surface 121, a second magnetic plate through hole 122, a second reaction tube through hole 13, a base 131, a sleeve 14, a support column 2, a magnetic plate 3, a reaction tube 31, a detachable part 32, a convex part 33 and a cover.
Detailed Description
The principles and features of this invention are described below in conjunction with the following drawings, which are set forth by way of illustration only and are not intended to limit the scope of the invention.
Example 1
As shown in FIGS. 1 to 4, the magnetic bead nucleic acid extraction apparatus of the present embodiment comprises a rack 1, a magnetic plate 2 and a reaction tube 3, wherein the magnetic plate 2 and the reaction tube 3 are detachably mounted on the rack 1,
the shelf 1 is formed in one piece and comprises a base 13, a first support surface 11 and a second support surface 12. The first supporting surface 11 is located above the second supporting surface 12, the first supporting surface 11 is provided with a reaction tube through hole 112 and a magnetic plate through hole 111, the reaction tube through hole 112 is close to the magnetic plate through hole 111, the reaction tube through hole 112 is circular, the second supporting surface 12 is provided with a second reaction tube through hole 122 corresponding to the reaction tube through hole 112 of the first supporting surface 11 and a second magnetic plate through hole 121 corresponding to the magnetic plate through hole 111, and the diameter of the second reaction tube through hole 122 is smaller than that of the reaction tube through hole 112.
The reaction tube 3 has a cylindrical structure with an open top, the upper portion of the reaction tube 3 has a cylindrical shape, the lower portion of the reaction tube 3 has a conical shape, the diameter of the cylindrical portion of the reaction tube 3 has a cross section equal to the maximum cross section of the conical portion, the diameter of the cylindrical portion of the reaction tube 3 has a cross section larger than the diameter of the second reaction tube through hole 122, the diameter of the reaction tube through hole 112 has a diameter equal to or slightly larger than the diameter of the upper portion of the reaction tube 3, such that a portion of the lower portion of the reaction tube 3 passes through the second reaction tube through hole 122, the lower portion of the reaction tube 3 is supported by the second supporting surface 12, and the first supporting surface. The magnetic plate 2 passes through the magnetic plate through hole 111 and the second magnetic plate through hole 121, the magnetic plate 2 is supported by the base 13, and the magnetic plate through hole 111 and the second magnetic plate through hole 121 confine the magnetic plate 2. The reaction tube 3 may also be provided with a cover to facilitate the blending operation.
The bottom of the reaction tube 3 is provided with a detachable part 31, after the detachable part 31 is detached, the bottom of the reaction tube 3 becomes open, in this example, the bottom of the reaction tube 3 is provided with an opening, the detachable part 31 is a plug adapted to the opening, the bottom of the reaction tube 3 is closed when the plug is plugged in the opening, and after the plug is removed, the bottom of the reaction tube 3 is open.
During the use, install magnetic sheet 2 and reaction tube 3 back on shelf 1, wait that the magnetic bead adsorbs on the inner wall of reaction tube 3, dismantle the stopper, put the supernatant, then plug with the stopper and plug up the opening, continue to carry out solution operation. The entire device need not be inverted to remove the supernatant.
Example 2
As shown in fig. 2 and 5, the other arrangement in this embodiment is the same as that in embodiment 1, except that the region of the reaction tube through hole 112 away from the magnetic plate through hole 112 is provided with a protrusion, and the outer wall of the reaction tube 3 is provided with a convex portion 32 corresponding to the protrusion, and the convex portion 32 can be clamped into the protrusion. The reaction tube is easy to rotate in the using process, so that the part adsorbing the magnetic beads is rotated away from the magnetic plate, and the magnetic beads and the nucleic acids carried on the magnetic beads enter the supernatant and are lost.
The bottom of the reaction tube 3 is provided with an external thread, and the detachable part 31 is screwed on the bottom of the reaction tube 3.
The detachable part 31 and the reaction tube 3 are in threaded connection, so that the operation is simple and convenient, the reaction tube is prevented from rotating in the use process by matching the protrusion and the convex part, and the effect is better.
Example 3
As shown in fig. 6, the other configuration of the present embodiment is the same as that of embodiment 1, except that the shelf 1 is not integrally formed, but a portion of the upper surface of the base 13 is detachable, the base 13 is provided with a sleeve 131, the first supporting surface 11, the second supporting surface 12 and the pillar 14 are integrally formed, the pillar 14 can be inserted into the sleeve 131, the sleeve 131 is provided with a limit screw, and the depth of the pillar 14 inserted into the sleeve 131 is limited as required, thereby adjusting the distance between the base 13 and the second supporting surface 12. The distance between the base and the second supporting surface can be adjusted according to specific conditions, so that a proper waste liquid container can be placed at the bottom of the reaction tube.
The device can be combined with a reagent for extracting magnetic bead nucleic acid into a magnetic bead nucleic acid extraction kit.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (7)
1. A device for magnetic bead nucleic acid extraction is characterized by comprising a frame (1), a magnetic plate (2) and a reaction tube (3), wherein the magnetic plate (2) and the reaction tube (3) are detachably arranged on the frame (1),
the shelf (1) comprises a base (13), a first supporting surface (11) and a second supporting surface (12), the first supporting surface (11) is positioned above the second supporting surface (12), and is fixedly connected with the base (13) through a strut, the second supporting surface (12) is connected with the base (13) through the strut, the first support surface (11) is provided with a reaction tube through hole (112) allowing the reaction tube (3) to pass through and a magnetic plate through hole (111) allowing the magnetic plate to pass through, the reaction tube through hole (112) is abutted against the magnetic plate through hole (111), the reaction tube through hole (112) is circular, the second supporting surface (12) is provided with a second reaction tube through hole (122) corresponding to the reaction tube through hole (112) of the first supporting surface (11), and a second magnetic plate through hole (121) corresponding to the magnetic plate through hole (111), and the diameter of the second reaction tube through hole (122) is smaller than the diameter of the reaction tube through hole (112);
the reaction tube (3) is of a cylindrical structure with an open top, the upper part of the reaction tube is cylindrical, the lower part of the reaction tube is conical, the cross-sectional diameter of the cylindrical part of the reaction tube (3) is equal to the maximum cross-sectional diameter of the conical part, and the cross-sectional diameter of the cylindrical part of the reaction tube (3) is larger than the diameter of the second reaction tube through hole (122);
the bottom of the reaction tube (3) is provided with a detachable part (31), and after the detachable part (31) is detached, the bottom of the reaction tube (3) is opened.
2. The device according to claim 1, characterized in that the reaction tube through hole (112) is provided with a protrusion in the area away from the magnetic plate through hole (111), and the outer wall of the reaction tube (3) is provided with a protrusion (32) corresponding to the protrusion, wherein the protrusion (32) can be clamped into the protrusion.
3. The device according to claim 1, characterized in that the bottom of the reaction tube (3) and the detachable member (31) are arranged in a plug-hole plug configuration.
4. The device according to claim 1, characterized in that the bottom of the reaction tube (3) is provided with an external thread, and the detachable member (31) is screwed to the bottom of the reaction tube (3).
5. Device according to claim 1, characterized in that the shelf (1) is arranged such that the distance between the base (13) and the second support surface (12) is adjustable.
6. The device according to any one of claims 1 to 5, characterized in that the reaction tube (3) is further provided with a cover (33).
7. A kit for magnetic bead nucleic acid extraction, comprising the device of any one of claims 1-6, and reagents for magnetic bead nucleic acid extraction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020084593.5U CN211921509U (en) | 2020-01-15 | 2020-01-15 | Device and kit for extracting magnetic bead nucleic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020084593.5U CN211921509U (en) | 2020-01-15 | 2020-01-15 | Device and kit for extracting magnetic bead nucleic acid |
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| CN211921509U true CN211921509U (en) | 2020-11-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202020084593.5U Active CN211921509U (en) | 2020-01-15 | 2020-01-15 | Device and kit for extracting magnetic bead nucleic acid |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113234589A (en) * | 2021-05-10 | 2021-08-10 | 宁波康程德诺生物医药有限公司 | Quadruple tube device, kit and extraction method for quickly extracting nucleic acid |
| CN113583865A (en) * | 2021-10-08 | 2021-11-02 | 深圳市瑞沃德生命科技有限公司 | Preparation method of separation column and separation column |
-
2020
- 2020-01-15 CN CN202020084593.5U patent/CN211921509U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113234589A (en) * | 2021-05-10 | 2021-08-10 | 宁波康程德诺生物医药有限公司 | Quadruple tube device, kit and extraction method for quickly extracting nucleic acid |
| CN113234589B (en) * | 2021-05-10 | 2024-03-26 | 宁波康程德诺生物医药有限公司 | Four-pipe device, kit and extraction method for rapid extraction of nucleic acid |
| CN113583865A (en) * | 2021-10-08 | 2021-11-02 | 深圳市瑞沃德生命科技有限公司 | Preparation method of separation column and separation column |
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