CN113583865A - Preparation method of separation column and separation column - Google Patents
Preparation method of separation column and separation column Download PDFInfo
- Publication number
- CN113583865A CN113583865A CN202111168575.0A CN202111168575A CN113583865A CN 113583865 A CN113583865 A CN 113583865A CN 202111168575 A CN202111168575 A CN 202111168575A CN 113583865 A CN113583865 A CN 113583865A
- Authority
- CN
- China
- Prior art keywords
- iron sand
- sorting
- column body
- sorting column
- polymer material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
Abstract
The embodiment of the invention provides a preparation method of a sorting column, which comprises the following steps: bonding iron sand through a polymer material and bonding the iron sand in the sorting column body; centrifuging the separation column body bonded with the iron sand under a first preset condition; baking the centrifuged separation column body under a second preset condition; the polymer material is formed by polymerizing monomers or is a high molecular polymer. According to the embodiment of the invention, the polymer material is used for bonding the iron sand, the iron sand is bonded in the sorting column body, then the sorting column body is sequentially centrifuged under the first preset condition, the sorting column body is baked under the second preset condition, the common polymer material and the specific preparation process are adopted to obtain the sorting column which is required by the cell sorting technology and meets the adsorption and sorting of the small magnetic beads, the preparation process is simple, and the cost is low.
Description
Technical Field
The invention relates to the technical field of cell sorting, in particular to a sorting column and a preparation method thereof.
Background
The cell sorting technology plays an important role in clinical diagnosis, cellular immunotherapy, cancer treatment and the like. In recent years, a method called CAR-T therapy (Chimeric Antigen Receptor T-Cell Immunotherapy) has been widely studied for cancer treatment, and has been found to be effective in clinical tumor treatment. A very important step in the CAR-T therapy of leukemia is T cell sorting, i.e., separation of mononuclear cells from the blood of tumor patients by peripheral hemocytometric separation, followed by magnetic bead purification using cell sorting technology.
Currently, the cell sorting techniques commonly used fall into two broad categories. The first type is large magnetic bead adsorption and sorting, where the large beads used are of a relatively large diameter, typically on the order of microns. The sorting process is that the large magnetic beads are adsorbed on the surface of target cells, and an external magnetic field acts on a common test tube containing a solution of various cells to attract the target cells with the large magnetic beads attached on the surface. However, the separation purity of the separation method is low, and is generally about 90%. The magnetic force of the large magnetic beads is large, and irreversible damage to active sites on the surface of target cells is possible; after sorting, an elution operation is required. The second type is the adsorption and sorting of small magnetic beads, the diameter of the used small magnetic beads is smaller, generally smaller than 100 nanometers, the magnetic beads have the characteristic of paramagnetism, and the magnetic beads are super-paramagnetic nano magnetic beads. In the sorting process, small magnetic beads are adsorbed on the surface of target cells, so that a solution containing various cells passes through the sorting column, and an external magnetic field acts on the sorting column to attract the target cells with the small magnetic beads attached to the surface. The sorting purity of the sorting method is high and can reach more than 95%. The magnetic force of the small magnetic beads is small, and the influence on active sites on the surface of target cells is small; and no elution operation is needed after sorting, which is beneficial to the subsequent experiment or treatment.
The cell sorting technology of small magnetic bead adsorption and sorting must use a sorting column to perform magnetic attraction, and the preparation flow of the sorting column is very complicated and has high price, which results in very high cost of cell sorting. These factors directly restrict the wide spread of cell sorting technology for small magnetic bead adsorption and sorting.
Disclosure of Invention
The embodiment of the invention provides a preparation method of a separation column, and aims to solve the problems of complex preparation process and high cost of the separation column in the prior art.
In a first aspect, a method for preparing a sorting column is provided, which comprises:
bonding iron sand through a polymer material and bonding the iron sand in the sorting column body;
centrifuging the separation column body bonded with the iron sand under a first preset condition;
baking the centrifuged separation column body under a second preset condition;
the polymer material is formed by polymerizing monomers or is a high molecular polymer, the monomers are acrylic monomers or styrene monomers, and the high molecular polymer is acrylic resin, polyurethane, epoxy or silica gel; the first preset condition is as follows: the centrifugal force is equal to 1000 times the acceleration of gravity, and the centrifugal time is 1-10 minutes; the second preset condition is as follows: the baking temperature is equal to 100 ℃, and the baking time is 3-10 hours.
In a second aspect, a sorting column is provided, which is prepared by the above method.
According to the embodiment of the invention, the polymer material is used for bonding the iron sand, the iron sand is bonded in the sorting column body, then the sorting column body is sequentially centrifuged under the first preset condition, the sorting column body is baked under the second preset condition, the common polymer material and the specific preparation process are adopted to obtain the sorting column which is required by the cell sorting technology and meets the adsorption and sorting of the small magnetic beads, the preparation process is simple, and the cost is low.
Drawings
The foregoing and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
FIG. 1 is a schematic diagram of a cell sorting technique with small magnetic bead adsorption and sorting;
FIG. 2 is a schematic diagram of a sorting column according to one embodiment of the present invention;
FIG. 3 is a flow chart of a method for preparing a sorting column according to the second embodiment of the present invention;
FIG. 4 is a flowchart of step S302 in FIG. 3 according to a second embodiment of the present invention;
fig. 5 is another flowchart of step S302 in fig. 3 according to a second embodiment of the present invention.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar modules or modules having the same or similar functionality throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention. On the contrary, the embodiments of the invention include all changes, modifications and equivalents coming within the spirit and terms of the claims appended hereto.
The small magnetic beads used in the cell sorting technology of small magnetic bead adsorption and sorting have small diameter, generally less than 100 nanometers. The magnetic beads have the characteristic of paramagnetism, are magnetized under an external magnetic field, and the magnetism can disappear immediately after the external magnetic field is removed, so the magnetic beads are super-paramagnetic nanoparticles.
FIG. 1 is a schematic of a cell sorting technique of small magnetic bead adsorption and sorting. The sorting process comprises the following steps: the separation column is matched with the external magnetic field for use, so that the external magnetic field acts on the region of the separation column filled with the iron sand. Under the action of an external magnetic field, an induction magnetic field is formed in the iron sand. The external magnetic field and the induction magnetic field are mutually superposed, and a superposed magnetic field which is equivalent to hundreds of times or even thousands of times of the external magnetic field is formed in the region of the separation column filled with the iron sand, so that the nano-scale small magnetic beads can be attracted. And (2) passing a solution containing various cells (containing target cells with small magnetic beads adsorbed on the surfaces) through a sorting column, wherein the target cells rapidly move and gather under the action of a superposed magnetic field when passing through a region filled with iron sand of the sorting column, so that cell sorting is realized.
Example one
Fig. 2 is a schematic diagram of a sorting column according to an embodiment of the present invention. As shown in fig. 2, the sorting column includes a sorting column body and iron sand. In the embodiment of the present invention, when the cell sorting is performed, the sorting column body is used as a container, and needs to have a certain degree of rigidity, at least, to be not deformed under an applied pressure of 5 kg. In addition, the sorting column body is made of medical plastics such as PS (Polystyrene), PP (polypropylene), PC (polycarbonate), PVC (Polyvinyl chloride) and the like, and meets the requirements of no toxicity and no harm to cells. Iron sand is filled in a certain area of the sorting column body, and the diameter of the iron sand is controlled between 50um and 500 um. The sorting column body includes a first portion, a second portion, and a tip. The three parts are all approximately cylindrical, are sequentially connected end to end and communicated, and have sequentially reduced diameters. I.e. the second portion is located between the first portion and the tip, the diameter of the second portion being between the diameter of the first portion and the diameter of the tip. And iron sand is filled in the second part, and when an external magnetic field is introduced into the second part, a superposed magnetic field is generated in the second part.
During cell sorting, a solution containing a plurality of cells (including target cells with small magnetic beads adsorbed on the surface) enters the sorting column from the inlet of the first section. When the iron sand is met when the iron sand enters the second part, the diameter of the second part is smaller than that of the first part, the flow rate of the solution is reduced, and the first part is equivalent to a container and can contain a part of the solution. After the solution enters the second part, the solution is subjected to the action of a superposed magnetic field, target cells with small magnetic beads adsorbed on the surfaces move directionally and are gathered on the iron sand, and other components flow through the gap of the iron sand and then flow out from the tip. The diameter of the tip is smaller than that of the second part, so that the flow rate of the solution can be further reduced, the time for the solution to flow through the iron sand is prolonged, and the influence of the superposed magnetic field on the target cells is more sufficient.
In order to prevent the iron sand from flowing out in the process of preparing the separation column, the round balls are placed into the separation column before the iron sand is filled. In an embodiment of the invention, the diameter of the ball is between the diameter of the second portion and the diameter of the tip, blocking the passage from the second portion to the tip. The material of the sphere includes, but is not limited to, metal, ceramic, glass, etc.
The iron sand filled in the second part, the bonding degree between the iron sand and between the iron sand and the second part are the key for determining whether the sorting column meets the cell sorting requirement. The sorting column of the embodiment of the present invention is prepared by the following preparation method of the embodiment, and detailed description thereof will not be provided.
Example two
Fig. 3 is a flow chart of a method for preparing a sorting column according to the second embodiment of the present invention. In the embodiment of the present invention, the structure of the sorting column is the same as that of the first embodiment, and the components that are the same as those of the first embodiment are given the same names as those of the first embodiment, and include all the features described in the first embodiment, which will not be described herein again. As shown in fig. 3, the method includes:
step S301: placing a round ball into the body of the sorting column, the diameter of the round ball being between the diameter of the second portion and the diameter of the tip.
Step S302: binding iron sand by a polymeric material and binding the iron sand within the sorting column body.
In the embodiment of the invention, the iron sand and the iron sand, and the iron sand and the second part of the sorting column body are connected through the polymer material. The polymer material is polymerized by monomers or is a high molecular polymer. Monomers include, but are not limited to, acrylic monomers or styrene monomers. The high molecular polymer includes, but is not limited to, acrylic, polyurethane, epoxy, or silicone polymers.
Besides the connection iron sand, the connection iron sand and the second part, the polymer material wraps the iron sand, so that the corrosion of moisture and oxygen to the iron sand can be prevented; and when the cells are sorted, the iron sand is prevented from being directly contacted with the cells, and the cells are prevented from being damaged by the release of harmful ions in the iron sand.
In order to shorten the wetting time of the iron sand, a hydrophilic substance having more hydrophilic groups may be added to the polymer material so that the polymer material includes the hydrophilic groups. Hydrophilic groups such as-COOH, -OH and-NH 2, and hydrophilic substances including, but not limited to, polyvinyl alcohol, polymaleic anhydride, polyvinyl pyrrolidone, polyquaternary ammonium salts or polyethylene glycol, and the like. After ultrapure water or a PBS solution (phosphate buffered saline) is added to the separation column prepared in the subsequent step, the wettability (wetting time) of the iron sand is good.
As an embodiment of the present invention, iron sand is bonded by a polymer material and the iron sand is bonded in a sorting column body, as shown in fig. 4, the method includes:
step S401: and filling iron sand in the second part of the sorting column body.
Step S402: a polymer material having fluidity is added to the iron sand.
In the embodiment of the invention, a specific quantitative filling device is adopted to fill the iron sand in the second part of the sorting column, then the polymer material which is not solidified yet, namely the polymer material with fluidity is added, and the polymer material slowly wraps the iron sand particles, and finally the subsequent centrifugation and baking steps are carried out.
As another embodiment of the present invention, iron sand is bonded by a polymer material and the iron sand is bonded in a sorting column body, as shown in fig. 5, the method comprising:
step S501: the iron sand and the polymer material are stirred in the vessel.
Step S502: and filling the second part of the sorting column body with uniformly stirred iron sand with fluidity and polymer materials.
In the embodiment of the invention, the iron sand and the polymer material which is not solidified are fully stirred uniformly in a container, then a mixture of the iron sand and the polymer material which is not solidified is filled in the second part of the sorting column by a specific quantitative filling device, and finally the subsequent steps of centrifuging and baking are carried out. The iron sand and the polymer material are stirred uniformly and refilled so that the polymer material can wrap the iron sand particles more fully and uniformly.
Step S303: and centrifuging the separation column body bonded with the iron sand under a first preset condition.
In the embodiment of the invention, the separation column body bonded with the iron sand is centrifuged under a first preset condition (the centrifugal force is equal to 1000 times the acceleration of gravity, and the centrifugation time is 1-10 minutes). At the moment, the mixture of the iron sand and the polymer material is still in an unset state, and the separation column body bonded with the iron sand is centrifuged for 1min to 10min under the centrifugal force of 1000g, so that the redundant polymer material is removed, and the distribution of the polymer material among the iron sand and the distribution of the iron sand in the second part are better and uniformly distributed. The first preset condition and the second preset condition of the subsequent baking jointly determine the adhesion degree between the iron sand and between the iron sand and the second part, and further influence the performance indexes of the sorting column, such as wetting time, cell yield, cell purity and the like.
Step S304: and baking the centrifuged separation column body under a second preset condition.
The baking temperature and baking time ultimately determine the degree of adhesion between the iron sand and the iron sand, and between the iron sand and the second part. In the embodiment of the invention, the centrifuged separation column body is baked under a second preset condition (baking temperature is equal to 100 ℃ and baking time is 3-10 hours). And after baking, the iron sand and the iron sand, and the iron sand and the second part have good binding force.
According to the embodiment of the invention, the polymer material is used for bonding the iron sand, the iron sand is bonded in the sorting column body, then the sorting column body is sequentially centrifuged under the first preset condition, the sorting column body is baked under the second preset condition, the common polymer material and the specific preparation process are adopted to obtain the sorting column which is required by the cell sorting technology and meets the adsorption and sorting of the small magnetic beads, the preparation process is simple, and the cost is low.
The sorting column prepared by the preparation method is compared with the existing sorting column under the same experimental conditions. The target cell of sorting is mouse spleen CD3 cell, and four performance indexes of comparison wetting time, single drop liquid dripping interval time, cell yield and positive sorting tube CD3 cell purity are provided.
The procedure for the preparation of single cell suspensions was as follows.
(1) Transferring the mouse spleen tissue to a cell filter screen, slightly grinding the tissue by using a flat end of a piston of a sterile syringe, adding a buffer solution while grinding, removing connective tissue and fat, collecting a single cell suspension obtained by grinding, centrifuging for 5min at the centrifugal force of 500g and at the temperature of 4 ℃, and then removing a supernatant.
(2) Adding 5ml of erythrocyte lysate, mixing, and cracking at 4 ℃ for 3-5 min.
(3) Adding 5 times of buffer solution, filtering with 70um filter membrane (wetting with 2ml buffer solution in advance), centrifuging at 500g centrifugal force and 4 deg.C for 5min, and removing supernatant; after resuspension, 5ml of buffer was added, and the mixture was centrifuged, and the supernatant was discarded.
(4) The cells were resuspended in 1.8 ml buffer, placed at 4 ℃ and counted to give a single cell suspension.
The cell labeling process is as follows.
(1) A portion was taken from the single cell suspension and the cell concentration was adjusted to 108 cells/ml.
(2) Resuspend 107 cells in a 2ml centrifuge tube with 100ul buffer.
(3) Add 10ul of biotinylated antibody (per 107 cells), mix well and incubate in the refrigerator for 10 min.
(4) 1ml of buffer was added, and the mixture was centrifuged at 500g for 5min to remove the supernatant.
(5) 80ul of buffer was added to resuspend the cells.
(6) Add 20ul of small avidin beads, mix well and incubate for 15 minutes in a refrigerator (2-C.).
(7) 1ml of buffer was added to 107 cells, centrifuged at 500g for 5min, and the supernatant was removed.
(8) The cells were resuspended with 2ml buffer, cell counting was performed, and cell sorting could then begin.
The cell sorting process is as follows.
(1) The column was placed in an externally applied magnetic field and washed with 2ml of buffer.
(2) And (3) adding the single cell suspension obtained in the cell marking process (8) into a sorting column, and collecting filtrate passing through the sorting column.
(3) The sorting column was washed with 2 x 2ml buffer and the filtrate was collected, containing cells without label, i.e. non-target cells, resulting in a negative sorting tube.
(4) And (4) taking out the sorting column from the external magnetic field, adding 2ml of buffer solution, pushing the needle tube type piston to wash the sorting column, and obtaining the positive marked cells. The solution collected at this time contained the target cells, resulting in a positive sort tube.
(5) The cell count of the negative sorting tube and the positive sorting tube was calculated using a cell counter.
(6) CD3 cell purity was detected in positive sorting tubes using a flow cytometer.
Comparative test results for four performance indicators are obtained as follows. As can be seen from the table, the sorting column of the invention is close to the existing sorting column in the four performance indexes of wetting time, single drop liquid dripping interval time, cell yield and positive sorting tube CD3 cell purity, has achieved the effect of the existing sorting column, and meets the requirements of the cell sorting technology of small magnetic bead adsorption and sorting.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Claims (10)
1. A method of preparing a sort column, the method comprising:
bonding iron sand through a polymer material and bonding the iron sand in the sorting column body;
centrifuging the separation column body bonded with the iron sand under a first preset condition;
baking the centrifuged separation column body under a second preset condition;
the polymer material is formed by polymerizing monomers or is a high molecular polymer, the monomers are acrylic monomers or styrene monomers, and the high molecular polymer is acrylic resin, polyurethane, epoxy or silica gel; the first preset condition is as follows: the centrifugal force is equal to 1000 times the acceleration of gravity, and the centrifugal time is 1-10 minutes; the second preset condition is as follows: the baking temperature is equal to 100 ℃, and the baking time is 3-10 hours.
2. The method of claim 1, wherein the sorting column body comprises a first portion, a second portion, and a tip; the second portion is located between the first portion and the tip, the second portion having a diameter between a diameter of the first portion and a diameter of the tip.
3. The method of claim 2, further comprising, prior to said binding iron sand by the polymeric material and binding the iron sand within the sorting column body:
placing a round ball into the sorting column body, the round ball having a diameter between the diameter of the second portion and the diameter of the tip.
4. The method of claim 3, wherein the binding iron sand by the polymeric material and within the sorting column body comprises:
filling iron sand in the second part of the sorting column body;
adding a polymer material having fluidity to the iron sand.
5. The method of claim 3, wherein the binding iron sand by the polymeric material and within the sorting column body comprises:
stirring iron sand and polymer material in a container uniformly;
and filling the second part of the sorting column body with uniformly stirred iron sand with fluidity and polymer materials.
6. The method of claim 3, wherein the material of the spherical balls is metal, ceramic or glass.
7. The method of claim 1, wherein the polymeric material comprises a hydrophilic substance.
8. The method according to claim 7, wherein the hydrophilic substance contains a hydrophilic group.
9. The method of claim 7, wherein the hydrophilic agent is polyvinyl alcohol, polymaleic anhydride, polyvinyl pyrrolidone, polyquaternary ammonium salt, or polyethylene glycol.
10. A sorting column, produced by the method of any one of claims 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111168575.0A CN113583865A (en) | 2021-10-08 | 2021-10-08 | Preparation method of separation column and separation column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111168575.0A CN113583865A (en) | 2021-10-08 | 2021-10-08 | Preparation method of separation column and separation column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113583865A true CN113583865A (en) | 2021-11-02 |
Family
ID=78242938
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111168575.0A Pending CN113583865A (en) | 2021-10-08 | 2021-10-08 | Preparation method of separation column and separation column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113583865A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6471860B1 (en) * | 1998-03-12 | 2002-10-29 | Miltenyi Biotech Gmbh | Magnetic micro separation column and method of using it |
| WO2003045565A1 (en) * | 2001-11-26 | 2003-06-05 | Miltenyi Biotec Gmbh | Apparatus and method for modification of magnetically immobilized biomolecules |
| CN1899698A (en) * | 2006-06-30 | 2007-01-24 | 上海师范大学 | Magnetic separating colume and its use in spearating biological samples |
| CN201857395U (en) * | 2010-10-14 | 2011-06-08 | 中国人民解放军军事医学科学院卫生装备研究所 | Sequential flow type automatic magnetic sorting device based on immune magnetic bead |
| CN107475071A (en) * | 2017-08-23 | 2017-12-15 | 清华大学 | Antibiotic for drug sensitive experiment encodes microchip and its preparation and detection method |
| CN107614458A (en) * | 2015-05-01 | 2018-01-19 | 百进生物科技公司 | stable nano magnetic particle dispersion |
| CN110121646A (en) * | 2016-12-28 | 2019-08-13 | 通用电气健康护理生物科学股份公司 | Magnetic immuno globulin-combines particle |
| US20200122134A1 (en) * | 2018-10-19 | 2020-04-23 | Vanderbilt University | Efficient capture of magnetic and paramagnetic carriers of biomarkers from a flowing system |
| CN211921509U (en) * | 2020-01-15 | 2020-11-13 | 广东腾飞基因科技股份有限公司 | Device and kit for extracting magnetic bead nucleic acid |
-
2021
- 2021-10-08 CN CN202111168575.0A patent/CN113583865A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6471860B1 (en) * | 1998-03-12 | 2002-10-29 | Miltenyi Biotech Gmbh | Magnetic micro separation column and method of using it |
| WO2003045565A1 (en) * | 2001-11-26 | 2003-06-05 | Miltenyi Biotec Gmbh | Apparatus and method for modification of magnetically immobilized biomolecules |
| CN1899698A (en) * | 2006-06-30 | 2007-01-24 | 上海师范大学 | Magnetic separating colume and its use in spearating biological samples |
| CN201857395U (en) * | 2010-10-14 | 2011-06-08 | 中国人民解放军军事医学科学院卫生装备研究所 | Sequential flow type automatic magnetic sorting device based on immune magnetic bead |
| CN107614458A (en) * | 2015-05-01 | 2018-01-19 | 百进生物科技公司 | stable nano magnetic particle dispersion |
| CN110121646A (en) * | 2016-12-28 | 2019-08-13 | 通用电气健康护理生物科学股份公司 | Magnetic immuno globulin-combines particle |
| CN107475071A (en) * | 2017-08-23 | 2017-12-15 | 清华大学 | Antibiotic for drug sensitive experiment encodes microchip and its preparation and detection method |
| US20200122134A1 (en) * | 2018-10-19 | 2020-04-23 | Vanderbilt University | Efficient capture of magnetic and paramagnetic carriers of biomarkers from a flowing system |
| CN211921509U (en) * | 2020-01-15 | 2020-11-13 | 广东腾飞基因科技股份有限公司 | Device and kit for extracting magnetic bead nucleic acid |
Non-Patent Citations (2)
| Title |
|---|
| 刘振宇: "《涂料涂装技术强制性标准认证全书》", 30 September 2002, 吉林摄影出版社 * |
| 廖树帜 编著: "《实用非金属材料手册》", 31 January 2011, 湖南科学技术出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU700437B2 (en) | Method of selection of a population or subpopulation of a sample | |
| CN105734043B (en) | Multi-sort cell separation method | |
| US20140302483A1 (en) | Cell separation method | |
| WO2015058206A1 (en) | Microfluidic sorting using high gradient magnetic fields | |
| WO1995028643A9 (en) | Method of selection of a population or subpopulation of a sample | |
| JP6485783B2 (en) | Medical inspection apparatus and cell inspection method | |
| EP3301443A1 (en) | Medical analysis device and cell analysis method | |
| JP2019138839A (en) | Specific cell capture method | |
| CN113583865A (en) | Preparation method of separation column and separation column | |
| JP4964161B2 (en) | Analytical method using magnetic particles | |
| CN109963600B (en) | Polymer for capturing or separating leukocytes, device, method for producing same, and use thereof | |
| CN210193892U (en) | Micro-fluidic chip | |
| JP6895662B2 (en) | Fractionation method and capture method of specific cells | |
| Rolland et al. | Flow cytometric quantitative evaluation of phagocytosis by human mononuclear and polymorphonuclear cells using fluorescent nanoparticles | |
| CN111621477A (en) | T cell sorting method | |
| CN113699038A (en) | Preparation method of separation column and separation column | |
| CN109239323A (en) | A kind of preparation method and applications of the immunomagnetic beads for whole blood T lymphocyte | |
| JPH02271867A (en) | Sealant for serum separation and blood separation method | |
| US20100173403A1 (en) | Non-isopycnic cell purification using percoll | |
| WO2021252926A1 (en) | Methods for preparation of immune cells | |
| JPH0558707B2 (en) | ||
| KR102298909B1 (en) | CTC Separation Method Using Counterflow Centrifugation | |
| CN111500441A (en) | Cell culture apparatus and cell culture method | |
| US20040248128A1 (en) | Method of analyzing interaction between material and biological substance | |
| TW201737984A (en) | Blood absorbent isolation material, blood filter device and operation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |