TW201737984A - Blood absorbent isolation material, blood filter device and operation method thereof - Google Patents

Abstract

A blood filter device includes a first cylinder, a blood filter material, a second cylinder, a blood absorbent particle material and a connection tube. The first cylinder has a first piston rod to contain blood therein while the second cylinder has a second piston rod to contain the blood therein. The blood filter material and the connection tube are provided between the first cylinder and the second cylinder for initially filtering the blood to form initially-filtered blood. The second cylinder contains the blood absorbent particle material therein to treat the initially-filtered blood to separate blood cells from plasma. In addition, the second cylinder further has a magnetic control device to attract the blood absorbent particle material which is made of a magnetic particle material to separate the blood-absorbed particle material.

Description

Blood adsorption separation material, blood filtration device and operation method thereof

The present invention relates to a blood adsorption separation material, a blood filtration device and an operation method thereof; in particular, to a blood adsorption precipitation material, a blood filtration device and an operation method thereof; more particularly, to a blood adsorption magnetic property [ Magnetic material, blood filtration device and method of operation thereof.

For example, a conventional blood separation method, such as the blood separation method and the invention patent of the Republic of China Patent Publication No. I395612, discloses a blood separation method. The blood separation method comprises: providing a first-class channel, a film having a plurality of holes, and a collecting groove, wherein the flow channel is stacked on the film, the collecting groove is disposed on the other side of the film; and driving a blood Flowing, and in contact with the film, moves substantially parallel to the film; the collection of the blood passes through one of the pores of the film. In addition, the blood cells in the blood are blocked by the film, and the other portion of the blood passes through the holes of the film, such that the portion of the blood that passes through the holes of the film is plasma.

The film of the above-mentioned No. I395612 has a first surface and a second surface, and the first surface has a plurality of curved surfaces. The holes are disposed between the curved faces, and the second surface corresponds to the holes, and has a plurality of recessed regions. The pores have a pore size ranging from 1 micron to 50 microns. The material of the film includes metals and/or alloys.

However, the blood separation method of the aforementioned No. I395612 is only The film is used, and the pores of the film have a pore size ranging from 1 micrometer to 50 micrometers. In fact, the aforementioned No. I395612 utilizes the film to block the filtration of blood cells in the blood and utilize the pores of the film to allow filtration through the plasma. Thus, the blood separation method of the aforementioned No. I395612 is only suitable for isolating general blood cells, and is not suitable for isolating platelets or blood cells. Thus, conventional blood separation devices and methods thereof necessarily have the potential to further provide for the isolation of platelets and blood cells.

Another conventional blood separation method, such as the blood separation device of the Republic of China Patent Publication No. M483797, discloses a blood separation device. The blood separation device comprises a sleeve, an inner rod assembly, a top cover and a bottom cover. The sleeve has a first end and a second end, and respectively defines a first opening and a second opening. The inner rod assembly is coaxially disposed in the sleeve in a sleeve manner, and the inner rod assembly includes a rod body and a groove body which are screwed to each other. The top cover sleeve closes the first opening, and the bottom cover sleeve closes the second opening. The bottom cover has a central through hole to allow the rod body to be connected to the groove. The blood separation device is used for separating platelet-rich plasma, and the same device can be used to perform blood extraction, centrifugation and extraction of specific blood stratification.

Another conventional blood separation method, such as the plasma collection device of the Republic of China Patent Publication No. M475927, discloses a plasma collection device. The plasma collection device comprises a tube body, a cover body, a piston and a push rod. The tube body sequentially forms a first tube body portion, a tube neck portion and a second tube body portion along an axial direction. The first tubular body portion has a first opening and an outer flange, and the second tubular body portion has a second opening, and the tubular neck portion connects the first tubular body portion and the second tubular body portion. The cover body covers the first opening, and the cover body has an inner plug portion and an outer ring portion, and the inner plug portion is received in the first tube body portion. Additionally, the outer ring portion abuts the outer flange such that the cover covers the first opening. The piston is disposed in the second tubular body portion to close the second opening. The push rod is disposed corresponding to the piston in the second The inside of the tube body, and the push rod slides in the axial direction against the piston to drive the piston to achieve adjustment of the blood stratification position. The plasma collection device is a centrifuge tube device for blood separation that allows blood separation to be performed in a centrifugal manner.

Another conventional blood separation method, such as the Separation of Autologous Blood Concentrator from the Republic of China Patent Publication No. M466716, discloses a separate autologous blood concentrator. The isolated autologous blood concentrator comprises an upper bottle and a lower bottle. The top of the lower bottle body has a plurality of convection holes, and the lower portion of the upper bottle body has a rotating control ring and a separating plate, and the rotating control ring is used to drive the separating plate to conduct or not to the lower bottle body. Convection hole. A vortex plug and a screw cap are disposed at a lower portion of the lower bottle body, and the height of the slewing plug is changed by the regulation of the screw cap. A cover or a sleeve is selected on the top of the upper bottle body, and a valve plug is sleeved inside the sleeve. The center of the valve plug is provided with a column tube to connect the column of the center of the sleeve. One side of the valve plug is further provided with a one-way valve, and a nozzle or a soft plug is disposed on one side of the sleeve. The isolated autologous blood concentrator centrifuges the blood into a plasma layer, a platelet thick plasma (PRP) layer, and a blood cell layer by gradient centrifugation.

However, the blood separation device of the above-mentioned No. M483797, the plasma collection device of No. M475927, and the isolated autologous blood concentrator of No. M466716 all use plasma separation to separate plasma and platelets, and separate the separation of plasma and platelets by centrifugation. The shortcomings of long time, high equipment cost and heavy weight.

Another conventional platelet separation device, such as the 〝 〝 platelet-taking device 〞 515 720, discloses a platelet separation device. The platelet separation device comprises a centrifugal separator, a first passage, a second passage, a plasma take-up bag, a platelet take-up bag, and a controller. The centrifugal separator has a blood storage space and a rotor, and the blood storage space is connected to the first-class inlet and the first-class outlet. According to the rotation of the rotor, the blood introduced from the inlet is centrifuged in the blood storage space. Ways to separate plasma from platelets. The first passage delivers blood to the centrifugal separator, and the second passage discharges the centrifugal separator, and the first passage and the second passage are connected to a plasma take-up bag.

According to the above, No. 515720, the centrifugal separator discharges plasma to the plasma taking bag via the second passage, and flows the plasma of the plasma taking bag back to the centrifugal separator via the first passage, so that the centrifugal separation is performed. Get platelets. The first passage has a blood donation pump and the second passage is connected to a platelet take-up pouch. The controller is for controlling the action of the rotor of the centrifugal separator and the action of the blood pump. Further, at the time of blood collection, the controller can change the number of revolutions of the rotor in accordance with the amount of blood inflow flowing into the centrifugal separator through the first passage.

However, the platelet separation device of the aforementioned No. 515720 uses the rotor to centrifugally separate plasma and platelets, and the separation of plasma and platelets by centrifugation has the disadvantages of excessive separation operation time, high equipment cost, and excessive weight.

Another conventional serum separation method, such as the filter film for filtering serum and a method for producing the same, and a filter device, and a device for filtering the serum, are disclosed in the Chinese Patent Publication No. I461230, which discloses a serum filtration separation method and apparatus therefor. The serum filtration separation device comprises a first substrate and at least one filtration membrane. The first substrate has a first through hole, and the filter film is disposed at a position opposite to the first through hole. The filter film has a plurality of filter holes, a first surface and a second surface, and the first surface has a plurality of curved faces, and the filter faces are between the curved faces, and the second surface corresponds to the first a surface, and the second surface has a plurality of recessed regions, and the recessed regions correspond to the filter holes.

However, the serum filtration separation device of the above-mentioned No. I461230 uses the filtration membrane to separate the serum by filtration, and the separation of the serum by filtration has the disadvantages of excessive filtration separation time, high equipment cost, and complicated work.

Another conventional blood separation method, such as the new blood cell plasma separation device of the Republic of China Patent No. M469483, discloses a blood cell plasma separation device comprising the same precipitation chamber, the same injection flow channel and a capillary separation microflow. Road. The sample precipitation chamber is configured to collect one of the blood samples to precipitate a blood cell, and the sample injection flow path is connected to the sample precipitation chamber for introducing the blood sample into the sample precipitation chamber. The capillary separation microchannel is connected between the sample injection flow channel and the sample precipitation chamber, and the capillary separation microchannel extends outward to the outer surface of the blood cell plasma separation device, and the blood sample is capillaryly acted upon. The plasma is exported.

However, the blood cell plasma separation device of the above-mentioned No. M469483 uses the capillary separation microchannel to separate blood cells and plasma by capillary separation, and separates blood cells and plasma by capillary separation, and has a long separation operation time and an excessively high equipment cost. The disadvantages of complicated work.

Therefore, there is a need for a further improvement in the conventional platelet separation device and method thereof. The foregoing patent publications No. I461230, No. I395612, No. M483797, No. M475927, No. M469483, No. M466716, and No. 515720 are merely references to the technical background of the present invention and the state of the art is not used. Limit the scope of the invention.

In view of the above, the present invention provides a blood adsorption separation material, a blood filtration device, and a method for operating the same, which utilizes a particulate material and a cell adhesion material to form a blood adsorption particulate material, and When the blood is separated, the cell-adhering material of the blood-adsorbing granular material can adsorb red blood cells, white blood cells and platelets, and the blood-adsorbing granular material accelerates the separation of blood by using sedimentation, so that the blood separation method can be greatly improved compared with the conventional blood separation method. Separation efficiency.

The main object of the present invention is to provide a blood adsorption separation material, a blood filtration device and an operation method thereof, which utilize a granular material and A cell-adhesive material is used to form a blood-adsorbing particulate material, and when the blood is separated, the cell-adhering material of the blood-adsorbing particulate material can adsorb red blood cells, white blood cells and platelets, and the blood-adsorbing particulate material is accelerated by sedimentation. Separate the blood for convenient operation and accelerated blood separation.

Another object of the present invention is to provide a blood adsorption separation material, a blood filtration device, and a method of operating the same, which use a magnetic particle material and a cell adhesion material to form a blood adsorption granular material, and when separating blood, The cell adhesion material of the blood adsorption granular material can adsorb red blood cells, white blood cells and platelets, and the blood adsorption granular material accelerates the separation of blood by magnetic adsorption to achieve convenient operation and accelerate blood separation operation.

In order to achieve the above object, a blood adsorption separation material according to a preferred embodiment of the present invention comprises: a particulate material having an outer surface; and a cell-adhesive material coated on an outer surface of the particulate material to form a The blood adsorbs the granular material, and the cell adhesive material is used for adhering red blood cells, white blood cells and platelets; wherein the cell material of the granular material has adhesive cell characteristics, and the cell adhesive material of the granular material is used to separate red blood cells and plasma .

In a preferred embodiment of the present invention, the cell-adhesive material comprises a first monomer material and a second monomer material, and the first monomer material and the second monomer material are selected from a single hydrophobic group. a primary amine, a secondary amine, a tertiary amine, a quaternary amine, a positively charged monomer, or any combination thereof, or the cell adhesion material is selected from a copolymerized polymer or a composite polymer containing a NIPAAM monomer. material.

In the preferred embodiment of the present invention, the composite polymer material is produced by radical polymerization of a first monomer material and a second monomer material. to make.

The particulate material of the preferred embodiment of the invention is selected from a high specific gravity particulate material or a magnetic particulate material.

In order to achieve the above object, a blood filter device according to a preferred embodiment of the present invention includes: a first tubular body having a first opening and a first piston push rod, and the first tubular body is for receiving a a blood filter material, disposed in the first opening; a second tubular body having a second opening and a second piston push rod, and the second tubular body is for receiving the blood; a blood adsorbing particulate material added to the second tubular body; and a connecting tube connected to the first opening and the second opening; wherein the blood filtering material is used to filter the blood to form a preliminary filtered blood, And the blood adsorbing particulate material is used for adsorbing and separating the blood to form a separated blood and an adsorbed blood globule.

In the preferred embodiment of the present invention, the second tubular body has a magnetic control device, and the blood adsorbing particulate material is a magnetic particulate material, and the adsorbed blood ball particles are magnetically separated by the magnetic control device.

In order to achieve the above object, a method for operating a blood filtering device according to a preferred embodiment of the present invention comprises: connecting a first tubular body and a second tubular body with a connecting pipe, and the first tubular body has a first a piston push rod, and the second tubular body has a second piston push rod; a blood filter material is connected to the first tubular body, and a blood adsorbing granular material is received in the second tubular body; The first piston push rod pushes a blood from the first tubular body through the blood filter material and the connecting tube to form a preliminary filtered blood, and delivers the preliminary filtered blood to the second tubular body; Using the blood adsorbing particulate material to adsorb and separate the preliminary filtered blood to form a separated blood and an adsorbed blood globule; and using the second piston push rod to separate the separated blood from the first tubular body The two-tube cylinder is reversely pushed back through the connecting tube and the blood filter material to form a backwashed filter substance blood, and the backwashed filter substance blood is transported back to the first tube column.

In the preferred embodiment of the present invention, the blood adsorbing particulate material is made of a particulate material and a cell adhesive material, and the cell adhesive material comprises a first monomer material and a second monomer material, and the first The monomer material and the second monomer material are selected from a monomer having a hydrophobic group, a primary amine, a secondary amine, a tertiary amine, a quaternary amine, a positively charged monomer, or any combination thereof, or the cell The adhesive material is selected from a copolymerized polymer containing a NIPAAM monomer or a composite polymeric material.

In the preferred embodiment of the present invention, the composite polymer material is formed by radical polymerization of a first monomer material and a second monomer material.

In the preferred embodiment of the present invention, the particulate material is selected from a high specific gravity particulate material or a magnetic particulate material to accelerate the separation of the blood by using the high specific gravity particulate material in a sedimentation manner, or to accelerate the separation by magnetic adsorption using the magnetic particulate material. The blood.

1‧‧‧Particulate materials

1a‧‧‧ Blood adsorption granular material

1b‧‧‧Adsorbed blood globules

2‧‧‧cell adhesion material

20‧‧‧cell adhesion coating

3‧‧‧Blood filter

31‧‧‧First pipe cylinder

311‧‧‧ first opening

312‧‧‧First Piston Putter

32‧‧‧Blood filter material

33‧‧‧Second tube

331‧‧‧ second opening

332‧‧‧Second Piston Putter

34‧‧‧Connecting tube

35‧‧‧Magnetic control device

Fig. 1 is a schematic view showing the construction of a particulate material, a cell-adhering material and a blood-adsorbing and separating material according to a preferred embodiment of the present invention.

Fig. 2 is a flow chart showing a method of producing a blood adsorption separation material according to a preferred embodiment of the present invention.

Fig. 3 is a side elevational view showing the preliminary filtration of blood by the blood filtering device of the first preferred embodiment of the present invention.

Fig. 4 is a side elevational view showing the completion of adsorption of blood cells by the particulate material of the blood filtering device of the first preferred embodiment of the present invention.

Fig. 5 is a side elevational view showing the hemorrhage of the blood filtering device of the first preferred embodiment of the present invention.

Fig. 6 is a side elevational view showing the preliminary filtration of blood by the blood filtering device of the second preferred embodiment of the present invention.

Fig. 7 is a side elevational view showing the granule material of the blood filtering device of the second preferred embodiment of the present invention completes the adsorption of blood cells.

Fig. 8 is a side elevational view showing the hemorrhage of the blood filtering device of the second preferred embodiment of the present invention.

In order to fully understand the present invention, the preferred embodiments of the present invention are described in detail below, and are not intended to limit the invention.

The blood adsorption separation material, the blood filtration device and the operation method thereof according to the preferred embodiment of the present invention are suitable for assembly and application to various related blood test devices or separation and extraction devices for various related blood components, for example, red blood cells, white blood cells and platelet separation devices, and extraction devices. Or a separation and extraction device, but it is not intended to limit the scope of application of the invention.

The blood adsorption separation material, the blood filtration device and the operation method thereof according to the preferred embodiment of the present invention are suitable for separating and extracting whole blood into various whole products or related products, for example, blood cell thick liquid, fresh frozen plasma, frozen sediment, and platelet thick. Liquid, white blood cell thick liquid or related articles, but it is not intended to limit the scope of application of the present invention.

The blood adsorption separation material, the blood filtration device and the operation method thereof according to the preferred embodiment of the present invention are suitable for use in various medical scientific researches, treating patients (for example, Achilles tendon rupture or treating arthritis), medical and aesthetic industries (for example: Treatment of baldness, reduction of wrinkles or increased collagen layer effects], medical rehabilitation (eg, surgery or post-implantation surgery), pharmaceutical products or related industries, but it is not intended to limit the scope of application of the present invention.

In general, the blood cell types of human blood include red blood cells, red corpuscle or erythrocyte, white blood cell or leukocyte, and platelets or platelets or thrombocytes. The diameter of human red blood cells is usually between about 6 μm and 8 μm. Human leukocytes contain granulocytes (granules) and granulocytes. The granulocyte includes a neutrophil (having a diameter of between about 12 μm and 15 μm), an eosinophilic ball (having a diameter of between about 12 μm and 15 μm), and a basophilic ball (having a diameter of between about 9 μm and 10 μm). . Agranulocyte-free white blood cells contain lymphocytes and monocytes. The diameter of the lymphocytes is between about 9 μm and 10 μm, while the diameter of the monocytes is between about 14 μm and 17 μm. Human platelets are between about 2 μm and 4 μm in diameter and have a thickness between about 0.2 μm and 1.5 μm .

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a schematic view showing the construction of a particulate material, a cell-adhering material and a blood-adsorbing and separating material according to a preferred embodiment of the present invention. Referring to FIG. 1, a blood adsorption separation material according to a preferred embodiment of the present invention comprises a particle material 1 and a cell adhesion material 2. Each of the particulate material 1 has an outer surface, and the cell adhesive material 2 can be coated on the outer surface of the particulate material 1 to form a blood adsorbing particulate material 1a, and the cell adhesive material 2 is used for dip Sticky red blood cells, white blood cells and platelets or other proteins.

Referring to FIG. 1 again, for example, in the preferred embodiment of the present invention, the cell adhesion material 2 of the particulate material 1 has the characteristics of adherent cells, and thus the cell adhesion characteristics of the cell adhesion material 2 It can be used to separate red blood cells and plasma.

Referring to FIG. 1 again, in another preferred embodiment of the present invention, the cell adhesive material 2 of the particulate material 1 has relatively high adhesion cell characteristics at a first operating temperature, and at a second operating temperature. At this time, the cell-adhesive material 2 of the particulate material 1 does not have adhesive cell characteristics or relatively low-viscous cell characteristics. The cell adhesive material 2 is selected from a group containing NIPAAM monomers. Copolymer or a composite polymer material. In another preferred embodiment of the present invention, the composite polymer material is formed by radical polymerization of a first monomer material and a second monomer material. The first monomer material and the second monomer material are selected from the group consisting of a monomer having a hydrophobic group, a primary amine, a secondary amine, a tertiary amine, a quaternary amine, a positively charged monomer, or any combination thereof. For example, the first monomer material is 0.1 to 99.9 weight percent of the composite polymer material, and the second monomer material is 0.1 to 99.9 weight percent of the composite polymer material. In another preferred embodiment of the present invention, the cell-adhesive material 2 is selected to comprise a first monomer material, a second monomer material, and a third monomer material.

2 is a schematic flow chart showing a method for producing a blood adsorption separation material according to a preferred embodiment of the present invention, which comprises four main steps S1 to S4, but is not intended to limit the sequence of steps of the present invention without departing from the scope of the present invention. The order of the steps of the preferred embodiment of the invention may be varied, divided, added, combined or reduced as appropriate.

Referring to Figures 1 and 2, the method for producing a blood adsorption separation material according to a preferred embodiment of the present invention comprises the step S1: First, the particulate material 1 is prepared in an appropriate manner when the material is prepared in advance. For example, the particulate material 1 is preferably selected from a variety of particulate materials, i.e., it may be selected from a high specific gravity particulate material or a magnetic particulate material.

Referring to FIGS. 1 and 2 again, the method for manufacturing a blood adsorption separation material according to a preferred embodiment of the present invention comprises the step S2: Next, when the material is prepared in advance, the cell adhesion material 2 is selectively added in a container. It is dissolved in a solvent (for example, an organic solvent or a solution containing a suitable organic solvent) to form a cell-adhesive coating 20.

Referring to FIGS. 1 and 2 again, the method for manufacturing a blood adsorption separation material according to a preferred embodiment of the present invention comprises the step S3: subsequently, the particulate material 1 is immersed in the cell adhesion coating 20, and is performed. The particulate material 1 and the cell-adhesive coating 20 are mixed to form a preliminary mixed particulate material.

Referring to FIGS. 1 and 2 again, the method for manufacturing a blood adsorption separation material according to the first preferred embodiment of the present invention comprises the step S4: Finally, the preliminary mixed particulate material is filtered, cleaned, and baked. Drying operation to obtain a blood adsorption separation material. At this time, the cell-adhesive material 2 may be coated on the outer surface of the particulate material 1 to form the blood-adsorbing particulate material 1a.

Fig. 3 is a side elevational view showing the preliminary filtration of blood by the blood filtering device of the first preferred embodiment of the present invention. Referring to FIG. 3, the blood filter device 3 of the first preferred embodiment of the present invention comprises a first tubular body 31, a blood filter material 32, a second tubular body 33, and a blood adsorbing particulate material 1a. And a connecting tube 34, and the first tubular body 31, the blood filter material 32, the second tubular body 33 and the connecting tube 34 are appropriately connected to constitute the blood filtering device 3.

Referring to FIG. 3, for example, the first tubular body 31 may be made of a transparent material or a semi-transparent material. The first tubular body 31 has a first opening 311 and a first piston pusher 312, and the first tubular body 31 is for accommodating a blood to be separated.

Referring to FIG. 3 again, for example, the blood filter material 32 is disposed in a unit body, and the blood filter material 32 is disposed between the first opening 311 and the connecting tube 34 to communicate the first A column of cylinders 31 and a connecting tube 34 are used to filter the blood to be separated by the blood filter material 32.

For example, the method for manufacturing the blood filter material 32 includes the steps of, for example, performing a radical polymerization reaction on a first monomer material and a second monomer material to form a composite polymer material. For example, the first monomer material is 0.1 to 99.9 weight of the composite polymer material. The percentage of the second monomer material is 0.1 to 99.9 weight percent of the composite polymer material. In another preferred embodiment of the invention, the blood filter material 32 is selected to comprise a first monomer material, a second monomer material, and a third monomer material.

For example, the first monomer material and the second monomer material are selected from a monomer that promotes platelet adsorption monomer, a hydrophobic group, or a functional group that adsorbs platelets, proteins, and promotes release of the platelets. a monomer material of the factor, and the first monomer material and the second monomer material are different monomer materials. The first monomer material and the second monomer material are platelet-adsorbing monomers selected from the group consisting of primary amines, secondary amines, tertiary amines, quaternary amines, positively charged monomers, or any combination thereof. The first monomer material is selected from the group consisting of long carbon chain containing monomers, dipropylene glycol methyl ether acrylate, propylene glycol methyl ether acrylate, isomers thereof, or any combination thereof. The monomer having a hydrophobic matrix is a soft ether-based acrylic compound having carbon chains of various lengths, and the carbon chain has a carbon number of 0 to 12.

For example, the method for manufacturing the blood filter material 32 includes the steps of: subsequently, for example, chemically precipitating and purifying the composite polymer material in a suitable treatment device to obtain a composite copolymerization. Composite polymer.

For example, the method of manufacturing the blood filter material 32 includes the steps of: subsequently dissolving the composite copolymer material with at least one organic solvent to form a composite copolymer material solution for subsequent processing operations, such as coating, dipping Or other processing operations.

For example, the method for manufacturing the blood filter material 32 includes the steps of: subsequently, using the composite copolymer material solution to form a platelet adsorption material, such as a blood separation gel, a blood separation coating, a hemostatic material, a blood separation material, and a wound promoting Healing materials or other medical related materials.

Please refer to Figure 3 again, for example, relative to the first A tubular body 31 has a second opening 331 and a second piston push rod 332, and the second tubular body 33 is for accommodating the blood. The blood filter material 32 and the connecting pipe 34 are disposed between the first tubular body 31 and the second tubular body 33. When the blood is initially filtered, the first piston push rod 312 is pushed into the blood to be separated in the first tubular body 31 to be transported through the first opening 311 as indicated by the right arrow in FIG. As such, the blood filter material 32 can be used to initially filter the blood to be separated to form a preliminary filtered blood.

Fig. 4 is a side elevational view showing the completion of adsorption of blood cells by the particulate material of the blood filtering device of the first preferred embodiment of the present invention. Referring to FIGS. 3 and 4, after the blood to be separated passes through the blood filter material 32, the preliminary filtered blood enters the second column 33. At this time, the blood adsorbing particulate material 1a has been added to the second tubular body 33, and the blood adsorbing particulate material 1a is for adsorbing and separating the preliminary filtered blood. When the blood adsorbing particulate material 1a is properly allowed to stand and naturally settles, a separated blood [plasma] and an adsorbed blood cell [erythrocyte] particle 1b are automatically separated, as shown in the lower half of Fig. 4.

Referring to Figures 3 and 4, the particulate material 1 is selected from a high specific gravity particulate material or a particulate material having a high specific gravity property to be naturally settled or artificially processed by the high specific gravity particulate material for a predetermined period of time. [Example: Centrifugal] Accelerate separation of the separated blood (located in the upper half of the second tubular body 33) and adsorbed blood globules 1b [deposited at the bottom of the second tubular body 33].

Fig. 5 is a side elevational view showing the blood filtering device of the first preferred embodiment of the present invention for performing backflushing to separate blood. Referring to FIG. 5, when the blood has been separated, the second piston push rod 332 is pushed into the separated blood in the second tubular body 33 to be transported through the second opening 331. Figure 5 shows the upward arrow. Thus, the separated blood can reversely flush back the filter material (eg, platelets) of the blood filter material 32, To form a backflushing filter blood, which can be used for subsequent processing. Finally, the backwashed filter substance blood is delivered back to the first column body 31. At this time, the second column 33 leaves only the adsorbed blood cell particles 1b, which is also available for subsequent processing.

Fig. 6 is a side elevational view showing the preliminary filtration of blood by the blood filtering device of the second preferred embodiment of the present invention, which corresponds to Fig. 3. Referring to FIG. 6, the blood filter device according to the second preferred embodiment of the present invention further includes a magnetic control device 35, and the magnetic control device 35 is disposed in the first embodiment. One side of the tubular body 33 or the surrounding area is provided to provide an appropriate magnetic field to control the blood absorbing particulate material 1a [or adsorbed blood globule particles 1b, as shown in Figures 7 and 8]. The blood-adsorbing particulate material 1a is a magnetic particulate material, and the magnetic control device 35 is used for magnetic attraction distribution or separation of the adsorbed blood-globule particles 1b in a subsequent process.

Referring to FIG. 6, for example, the magnetic field of the magnetron 35 can selectively control the blood adsorbing particulate material 1a to be appropriately distributed in the second tubular body 33 by magnetic attraction. Alternatively, the magnetic field of the magnetron 35 may be selected to magnetically control the adsorbed blood cell particles 1b (as shown in Figures 7 and 8) to be properly distributed in the bottom or other specific regions of the second tubular body 33.

Fig. 7 is a side elevational view showing the completion of adsorption of blood cells by the particulate material of the blood filtering device of the second preferred embodiment of the present invention, which corresponds to Fig. 4. Referring to FIG. 7, after the blood to be separated passes through the blood filter material 32, the preliminary filtered blood enters the second column 33. At this time, the blood adsorbing particulate material 1a has been added to the second tubular body 33, and the blood adsorbing particulate material 1a is for adsorbing and separating the preliminary filtered blood. When the magnetic field of the magnetron 35 controls the blood adsorbing particulate material 1a to settle or accumulate at a predetermined position by magnetic attraction, a separated blood and an adsorbed blood cell particle 1b can be automatically separated and formed (as shown in FIG. 8). Lower half Shown].

Fig. 8 is a side elevational view showing the blood filtering device of the second preferred embodiment of the present invention for performing backflushing to separate blood, which corresponds to Fig. 5. Referring to FIG. 8, when the blood has been separated, the magnetic control device 35 is not required to be operated, and only the second piston push rod 332 is pushed into the separated blood in the second tubular body 33. To pass through the second opening 331, as indicated by the upward arrow in FIG. Thus, the separated blood can reversely flush back the filter material (e.g., platelets) of the blood filter material 32 to form the blood of the backwash filter material, which can be used for subsequent processing. Finally, the backwashed filter substance blood is delivered back to the first column body 31. At this time, the second column 33 leaves only the adsorbed blood cell particles 1b, which is also available for subsequent processing.

The foregoing preferred embodiments are merely illustrative of the invention and the technical features thereof, and the techniques of the embodiments can be carried out with various substantial equivalent modifications and/or alternatives; therefore, the scope of the invention is subject to the appended claims. The scope defined by the scope shall prevail. The copyright limitation of this case is used for the purpose of patent application in the Republic of China.

1a‧‧‧ Blood adsorption granular material

3‧‧‧Blood filter

31‧‧‧First pipe cylinder

311‧‧‧ first opening

312‧‧‧First Piston Putter

32‧‧‧Blood filter material

33‧‧‧Second tube

331‧‧‧ second opening

332‧‧‧Second Piston Putter

34‧‧‧Connecting tube

Claims (10)

A blood adsorption separation material comprising: a particulate material having an outer surface; and a cell-adhesive material coated on an outer surface of the particulate material to form a blood-adsorbing particulate material, and the cell is stained The adhesive material is used for adhering red blood cells, white blood cells and platelets; wherein the cell material of the granular material has adhesive cell characteristics, and the cell adhesive material of the granular material is used to separate red blood cells and plasma. The blood adsorption separation material according to claim 1, wherein the cell adhesion material comprises a first monomer material and a second monomer material, and the first monomer material and the second monomer material are selected. From a monomer having a hydrophobic group, a primary amine, a secondary amine, a tertiary amine, a quaternary amine, a positively charged monomer, or any combination thereof, or the cell adhesion material is selected from a NIPAAM containing monomer Copolymer or a composite polymer material. The blood adsorption separation material according to claim 2, wherein the composite polymer material is formed by radical polymerization of a first monomer material and a second monomer material. The blood adsorption separation material according to claim 1, wherein the particulate material is selected from a high specific gravity particulate material or a magnetic particulate material. A blood filtering device comprising: a first tubular body having a first opening and a first piston push rod, wherein the first tubular body is for accommodating a blood; a blood filtering material is disposed The second opening; a second tubular body having a second opening and a second piston push rod, wherein the second tubular body is for accommodating the blood; a blood absorbing particulate material is added to The second tubular body; and a connecting tube connected to the first opening and the second opening; wherein the blood filtering material is used to filter the blood to form a preliminary filtered blood, and the blood adsorbs the particulate material for adsorption The blood is separated to form a separated blood and an adsorbed blood globule. The blood filter device of claim 5, wherein the second tubular body has a magnetic control device, and the blood adsorbing particulate material is a magnetic particulate material, and the magnetic control device is used for magnetic separation. Blood cell particles have been adsorbed. A method for operating a blood filtering device, comprising: connecting a first tubular body and a second tubular body by a connecting pipe, wherein the first tubular body has a first piston push rod, and the second pipe The cylinder has a second piston push rod; a blood filter material is connected to the first tubular body, and a blood adsorbing granular material is accommodated in the second tubular body; and a blood is used by the first piston push rod The first tubular body is pushed through the blood filter material and the connecting tube to form a preliminary filtered blood, and the preliminary filtered blood is delivered to the second tubular body; the second tubular body utilizes the blood adsorption The particulate material is subjected to adsorption separation to initially filter the blood to form a separated blood and an adsorbed blood globule; and the second piston push rod is used to push the separated blood backward from the second tubular body through the connecting tube And the blood filtering material to form a backwashing substance blood, and transporting the backwashing substance blood back to the first column. The method for operating a blood filtering device according to the seventh aspect of the invention, wherein the blood absorbing particulate material is made of a granular material and a cell-adhesive material, and the cell-adhesive material comprises a first monomer material and a second monomer material, and the first monomer material and the second monomer material are selected from a monomer having a hydrophobic group, a primary amine, a secondary amine, a tertiary amine, a quaternary amine, and a positively charged The monomer or any composition thereof, or the cell adhesion material is selected from a copolymerized polymer containing a NIPAAM monomer or a composite polymeric material. The method of operating a blood filter device according to claim 8, wherein the composite polymer material is formed by radical polymerization of a first monomer material and a second monomer material. The method of operating a blood filtering device according to claim 8, wherein the particulate material is selected from a high specific gravity particulate material or a magnetic particulate material to accelerate separation of the blood by sedimentation using the high specific gravity particulate material, or The magnetic particle material is used to accelerate the separation of the blood by magnetic adsorption.