CN211801007U - Centrifuging tube electromagnetic force support - Google Patents

Abstract

The utility model discloses a centrifuging tube electromagnetic force support, including erecting support, last horizontal support, going up the fixed orifices, horizontal magnetic support, lower fixed orifices, electromagnetic means, switch and supply socket down, perpendicular support constitute by the same square panel of two piece sizes, last horizontal support be rectangle plate structure, last fixed orifices be 12 apertures of evenly distributed on last horizontal support, lower horizontal magnetic support constitute by rectangular soft iron board, lower fixed orifices be evenly distributed 12 apertures on horizontal magnetic support down to with last fixed orifices one-to-one, there is a soft iron cylinder below horizontal magnetic support positive center down, electromagnetic means constitute by copper enameled wire circle and the soft iron cylinder under horizontal magnetic support down. The utility model discloses an utilize the principle of electro-magnet, can control magnetic force at any time and have or not, reach the control to the magnetic bead, effect that goes on in batches when realizing the washing can improve work efficiency greatly.

Description

Centrifuging tube electromagnetic force support

Technical Field

The utility model belongs to the technical field of molecular biology, mainly relate to a centrifuging tube electromagnetic force support.

Background

The magnetic bead method for extracting nucleic acid can be generally divided into four steps: cracking, combining, washing and eluting. The magnetic bead method for extracting nucleic acid is to crack sample cells by using a cell lysis solution, nucleic acid molecules dissociated from the cells are specifically adsorbed to the surfaces of the magnetic beads to form magnetic bead-nucleic acid polymers, and impurities such as protein and the like are not adsorbed and remain in the solution. After reacting for a certain time, under the action of a magnetic field, separating the magnetic bead-nucleic acid polymer from the liquid, recovering the magnetic bead-nucleic acid polymer, and then eluting with an eluent to obtain pure nucleic acid. The magnetic bead method does not need to add various reagents and centrifugation, and is an important direction for developing a future nucleic acid purification method. The magnetic bead method needs to use a PCR tube magnetic frame, the existing PCR tube magnetic frame is of a structure that a permanent magnet block and a support are integrated, although the magnetic bead-nucleic acid polymer can be rapidly gathered, in the washing and elution process, the magnetic bead-nucleic acid polymer cannot be dispersed due to the permanent magnetic effect of a magnet, and the PCR tubes on the PCR tube magnetic frame need to be taken down one by one and shaken to enable the magnetic bead-nucleic acid polymer to obtain washing liquid or eluent, which is very inconvenient. Therefore, a magnetic frame of a PCR tube capable of regulating and controlling a magnetic field is very needed at present.

Disclosure of Invention

An object of the utility model is to provide a centrifuging tube electromagnetic force support can accomplish the nucleic acid extraction test effectively, fast and safely, the utility model discloses used the electro-magnet, can control the effect that whether magnetic field goes on in batches when realizing the washing to the effect of magnetic bead as required, can also improve work efficiency.

Therefore, the technical scheme is that the utility model provides a centrifuge tube electromagnetic force support, including vertical support, last horizontal support, last fixed orifices, lower horizontal magnetic support, lower fixed orifices, electromagnetic means, switch and supply socket, vertical support constitute by two pieces size the same square panel, last horizontal support be rectangle panel structure, last fixed orifices evenly distributed 12 apertures on last horizontal support, lower horizontal magnetic support constitute by rectangular soft iron plate, lower fixed orifices evenly distributed 12 apertures on lower horizontal magnetic support to with last fixed orifices one-to-one, there is a soft iron cylinder below the positive center of lower horizontal magnetic support, vertical support, last horizontal support, lower horizontal magnetic support be by the screw fixation and shaping, last horizontal support and lower horizontal magnetic support between have a certain distance, the electromagnetic device is composed of an enameled coil made of copper and a soft iron cylinder right below the lower transverse magnetic support, one end of the enameled coil of the electromagnetic device is connected with one end of a switch, the other end of the switch is connected with one end of a 12V direct-current power socket, and the other end of the enameled coil of the electromagnetic device is connected with the other end of the 12V direct-current power socket. The side length of the vertical support is 60-64mm, the thickness is 2-3mm, the length of the upper transverse support is 130-136mm, the width is 50-54mm, the thickness is 2-3mm, the diameter of the upper fixing hole is 10.5-11mm, the length of the lower transverse magnetic support is 130-136mm, the width is 50-54mm, the thickness is 2-3mm, the distance between the upper transverse support and the lower transverse magnetic support is 24-28mm, the diameter of the lower fixing hole is 4.8-5.0mm, the diameter of the soft iron cylinder below the center of the lower transverse magnetic support is 10-12mm, and the height is 15-17 mm. The vertical support and the upper horizontal support are made of aluminum alloy or medical plastic.

The utility model discloses an utilize the principle of electro-magnet, can control magnetic force at any time and have or not, reach the control to the magnetic bead, effect that goes on in batches when realizing the washing can also improve work efficiency.

The utility model discloses technical scheme has following advantage: the novel solar water heater has the advantages of small volume, light weight, simple structure, low price, safe and reliable use, great saving of manpower and material resources, environmental protection and capability of meeting market demands.

Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by the practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and claims hereof as well as the appended drawings.

The technical solution of the present invention is further described in detail by the accompanying drawings and examples.

Drawings

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention.

Fig. 1 is a schematic structural diagram of the present invention.

In the figure, 1 stands vertically; 2, an upper transverse bracket; 3, fixing holes are formed; 4, a lower transverse magnetic bracket; 5, lower fixing holes; 6 an electromagnetic device; 7, a switch; 8, a power socket; 9 soft iron cylinder.

Detailed Description

In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the detailed description.

In the description of the present application, it is to be understood that the terms "intermediate," "top," "bottom," "inner," "outer," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the present application and simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed in a particular orientation, and be operated in a particular orientation, and thus should not be construed as limiting the present application. The terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.

In addition, unless expressly stated or limited otherwise, the terms "mounted," "connected," "secured," and the like are intended to be inclusive and mean, for example, that there may be a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.

In this application, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact of the first and second features, or may comprise contact of the first and second features not directly but through another feature in between. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

Referring to the drawings, the utility model provides a centrifuge tube electromagnetic force support, which comprises a vertical support 1, an upper horizontal support 2, an upper fixing hole 3, a lower horizontal magnetic support 4, a lower fixing hole 5, an electromagnetic device 6, a switch 7 and a power socket 8, wherein the vertical support 1 is composed of two square plates with the same size, the upper horizontal support 2 is of a rectangular plate structure, the upper fixing hole 3 is formed by uniformly distributing 12 small holes on the upper horizontal support 2, the lower horizontal magnetic support 4 is formed by rectangular soft iron plates, the lower fixing hole 5 is formed by uniformly distributing 12 small holes on the lower horizontal magnetic support 4 and is in one-to-one correspondence with the upper fixing hole 3, a soft iron column 9 is arranged below the center of the lower horizontal magnetic support 4, the vertical support 1, the upper horizontal support 2 and the lower horizontal magnetic support 4 are formed by screw fixation, a certain distance is arranged between the upper horizontal support 2 and the lower horizontal magnetic support 4, the electromagnetic device 6 is composed of an enameled coil made of copper and a soft iron cylinder 9 right below the lower transverse magnetic support 4, one end of the enameled coil of the electromagnetic device 6 is connected with one end of a switch 7, the other end of the switch is connected with one end of a 12V direct-current power socket 8, and the other end of the enameled coil of the electromagnetic device 6 is connected with the other end of the 12V direct-current power socket 8. The side length of the vertical support 1 is 60-64mm, the thickness is 2-3mm, the length of the upper transverse support 2 is 130-136mm, the width is 50-54mm, the thickness is 2-3mm, the diameter of the upper fixing hole 3 is 10.5-11mm, the length of the lower transverse magnetic support 4 is 130-136mm, the width is 50-54mm, the thickness is 2-3mm, the distance between the upper transverse support 2 and the lower transverse magnetic support 4 is 24-28mm, the diameter of the lower fixing hole 5 is 4.8-5.0mm, the diameter of the soft iron cylinder 9 at the center below the middle of the lower transverse magnetic support 4 is 10-12mm, and the height is 15-17 mm. The vertical support 1 and the upper transverse support 2 are made of aluminum alloy or medical plastic.

When the utility model is used, firstly, an operator inserts a 12V direct current power supply on a power socket 8, then samples of nucleic acid to be extracted are respectively added into a centrifuge tube, and the centrifuge tube is respectively inserted from an upper fixing hole 3 of an upper cross support 2 of the utility model, so that the centrifuge tube is fixed in the upper fixing hole 3 and a lower fixing hole 5, then lysate is respectively added, after 5-10 minutes, magnetic beads are respectively added, after 1-2 minutes, the utility model is held by hands and lightly shaken or put on an oscillator to lightly shaken to fully combine the magnetic beads and the nucleic acid polymer to form the magnetic beads-nucleic acid polymer, at the moment, a switch 7 is opened, an electromagnetic device 6 generates magnetic force through a soft iron cylinder 9 and a lower cross magnetic support 4, the magnetic beads-nucleic acid polymer is adsorbed at the bottom of the centrifuge tube, at the moment, a sample adding device is used for absorbing away liquid containing impurities such as protein, add the washing liquid again, close switch 7, the magnetic force that electromagnetic means 6 produced through soft iron cylinder 9 and lower transverse magnetic force support 4 disappears, will the utility model discloses hold gently to rock or put on the oscillator gently and make the abundant washing of nucleic acid sample liquid and magnetic ball and nucleic acid polymer fully combine, form magnetic ball-nucleic acid polymer, open switch 7 again after 2-3 minutes, electromagnetic means 6 produces magnetic force through soft iron cylinder 9 and lower transverse magnetic force support 4, adsorb magnetic ball-nucleic acid polymer at the bottommost of centrifuging tube, reuse injector will contain the liquid of washing liquid and inhale away, add the eluant again, close switch 7, electromagnetic means 6 disappears through the magnetic force that soft iron cylinder 9 and lower transverse magnetic force support 4 produced, will the utility model discloses hold gently to rock or put on the oscillator gently and make the abundant washing of nucleic acid sample liquid and magnetic ball and nucleic acid polymer fully elute 2-3 minutes The magnetic beads and the nucleic acid polymer are fully separated, the switch 7 is turned on again, the electromagnetic device 6 generates magnetic force through the soft iron cylinder 9 and the lower transverse magnetic support 4, the magnetic beads are adsorbed at the bottommost part of the centrifugal tube, the liquid containing the eluent is sucked away by the sample injector, and the eluent contains the nucleic acid in the sample and is used for nucleic acid detection.

The utility model discloses an utilize the principle of electromagnet, can control magnetic force at any time and have or not, reach the control to the magnetic bead, effect that goes on in batches when realizing the washing, moreover small, light in weight, simple structure, low price and use safe and reliable have practiced thrift manpower and material resources greatly, and green accords with the market demand moreover.

It should be noted that the above embodiments are all preferred embodiments, relevant functional components may be replaced by other components, and the related units and modules are not necessarily required by the present application. The embodiments in the present specification are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.

The centrifuge tube electromagnetic force bracket provided by the application is described in detail above, and specific examples are applied in the description to explain the principle and the implementation of the application, and the description of the examples is only used to help understand the method and the core idea of the application; meanwhile, for a person skilled in the art, according to the idea of the present application, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present application.

Claims (3)

1. The utility model provides a centrifuging tube electromagnetic force support which characterized in that: the magnetic force sensor comprises a vertical support, an upper transverse support, an upper fixing hole, a lower transverse magnetic support, a lower fixing hole, an electromagnetic device, a switch and a power socket, wherein the vertical support is composed of two square plates with the same size, the upper transverse support is of a rectangular plate structure, 12 small holes are uniformly distributed in the upper transverse support, the lower transverse magnetic support is composed of rectangular soft iron plates, 12 small holes are uniformly distributed in the lower transverse magnetic support and correspond to the upper fixing hole one by one, a soft iron cylinder is arranged below the center of the lower transverse magnetic support, the vertical support, the upper transverse support and the lower transverse magnetic support are formed by fixing screws, a certain distance is reserved between the upper transverse support and the lower transverse magnetic support, and the electromagnetic device is composed of a copper enameled coil and a soft iron cylinder arranged right below the lower transverse magnetic support, one end of the enameled coil of the electromagnetic device is connected with one end of the switch, the other end of the switch is connected with one end of the 12V direct-current power socket, and the other end of the enameled coil of the electromagnetic device is connected with the other end of the 12V direct-current power socket.

2. The centrifuge tube electromagnetic force bracket of claim 1, which is characterized in that: the side length of the vertical support is 60-64mm, the thickness is 2-3mm, the length of the upper transverse support is 130-136mm, the width is 50-54mm, the thickness is 2-3mm, the diameter of the upper fixing hole is 10.5-11mm, the length of the lower transverse magnetic support is 130-136mm, the width is 50-54mm, the thickness is 2-3mm, the distance between the upper transverse support and the lower transverse magnetic support is 24-28mm, the diameter of the lower fixing hole is 4.8-5.0mm, the diameter of the soft iron cylinder below the center of the lower transverse magnetic support is 10-12mm, and the height is 15-17 mm.

3. The centrifuge tube electromagnetic force bracket of claim 1, which is characterized in that: the vertical support and the upper horizontal support are made of aluminum alloy or medical plastic.

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