CN110945125A - 用于改进大肠杆菌的htp基因工程改造平台 - Google Patents

用于改进大肠杆菌的htp基因工程改造平台 Download PDF

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CN110945125A
CN110945125A CN201880047657.XA CN201880047657A CN110945125A CN 110945125 A CN110945125 A CN 110945125A CN 201880047657 A CN201880047657 A CN 201880047657A CN 110945125 A CN110945125 A CN 110945125A
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coli strain
gene
library
coli
strain
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Z·塞尔贝尔
E·J·迪安
S·曼彻斯特
K·戈拉
E·谢尔曼
A·金博尔
S·希捷卡
B·弗勒旺
T·特赖诺尔
M·弗拉斯曼
R·豪斯哈尔特
S-A·摩根
M·布莱斯
P·拉马克里希南
K·罗特席尔德-曼奇内里
Y·基姆
M·戴维斯
C·威斯纳维斯基
P·韦斯特福尔
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Zymergen Inc
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences

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CN201880047657.XA 2017-06-06 2018-06-06 用于改进大肠杆菌的htp基因工程改造平台 Pending CN110945125A (zh)

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US201762515870P 2017-06-06 2017-06-06
US62/515,870 2017-06-06
PCT/US2018/036333 WO2018226880A1 (en) 2017-06-06 2018-06-06 A htp genomic engineering platform for improving escherichia coli

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EP (2) EP3485013B1 (https=)
JP (1) JP2020524490A (https=)
KR (1) KR20200026874A (https=)
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CA (1) CA3064612A1 (https=)
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CN110272907A (zh) * 2019-08-12 2019-09-24 华中农业大学 一种调控番茄茎杆发育的基因sd1及其应用
CN111612133A (zh) * 2020-05-20 2020-09-01 广州华见智能科技有限公司 基于人脸图像多阶段关系学习的内脏器官特征编码方法
CN112941058A (zh) * 2021-04-02 2021-06-11 重庆科润生物医药研发有限公司 一种重组溶组织梭菌ii型胶原酶及其制备方法和应用
CN113265412A (zh) * 2021-05-20 2021-08-17 北京化工大学 多酶偶联定位体系及其构建方法和应用
CN113393900A (zh) * 2021-06-09 2021-09-14 吉林大学 基于改进Transformer模型的RNA状态推断研究方法
CN114672505A (zh) * 2022-03-15 2022-06-28 江苏省中国科学院植物研究所 一种功能性表达细胞色素p450酶的方法及其应用
CN114921463A (zh) * 2022-04-11 2022-08-19 中国农业科学院生物技术研究所 一种调控萜类化合物合成产量的人工非编码RNA分子CsiY及其应用
CN115074410A (zh) * 2022-06-30 2022-09-20 武汉合生科技有限公司 一种生物合成虾青素的方法及载体
CN115992160A (zh) * 2022-09-30 2023-04-21 倍生生物科技(深圳)有限公司 一种穿梭载体及其构建方法和应用
CN116013407A (zh) * 2022-12-26 2023-04-25 浙江大学杭州国际科创中心 一种基于语言模型的性质解耦蛋白质生成方法
CN116504313A (zh) * 2023-05-08 2023-07-28 暨南大学 基因终止子查询方法、装置、设备及可读存储介质
CN117275590A (zh) * 2023-11-10 2023-12-22 华东师范大学 有机固废体系中大分子的降解功能基因数据库与分析平台

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SG11202104409YA (en) 2018-10-31 2021-05-28 Zymergen Inc Multiplexed deterministic assembly of dna libraries
CN113728106A (zh) * 2019-03-08 2021-11-30 齐默尔根公司 微生物中的迭代基因组编辑
US11053515B2 (en) 2019-03-08 2021-07-06 Zymergen Inc. Pooled genome editing in microbes
US11526705B2 (en) * 2019-04-01 2022-12-13 Lg Electronics Inc. Method of classificating outlier in object recognition and device and robot of classifying thereof
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JPWO2022124302A1 (https=) * 2020-12-08 2022-06-16
US11530406B1 (en) 2021-08-30 2022-12-20 Sachi Bioworks Inc. System and method for producing a therapeutic oligomer
US12037621B2 (en) 2021-09-15 2024-07-16 Archer-Daniels-Midland Company Threonine production strain having attenuated expression of the yafV gene
CN115820703A (zh) * 2022-12-20 2023-03-21 山东乾肽生物科技有限公司 利用rna环化技术制备寡肽的方法及寡肽的表达dna
KR20250069469A (ko) * 2023-11-10 2025-05-19 한국과학기술원 TdT(Terminal deoxynucleotidyl transferase) 및 dNTP를 이용한 비번역영역 (untranslated region) 라이브러리 합성 방법
CN117238380A (zh) * 2023-11-15 2023-12-15 中国医学科学院北京协和医院 宏基因组病原识别的ai推荐方法、装置、设备及介质

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CN110272907B (zh) * 2019-08-12 2021-04-23 华中农业大学 一种调控番茄茎杆发育的基因sd1及其应用
CN110272907A (zh) * 2019-08-12 2019-09-24 华中农业大学 一种调控番茄茎杆发育的基因sd1及其应用
CN111612133A (zh) * 2020-05-20 2020-09-01 广州华见智能科技有限公司 基于人脸图像多阶段关系学习的内脏器官特征编码方法
CN112941058B (zh) * 2021-04-02 2023-12-05 重庆科润生物医药研发有限公司 一种重组溶组织梭菌ii型胶原酶及其制备方法和应用
CN112941058A (zh) * 2021-04-02 2021-06-11 重庆科润生物医药研发有限公司 一种重组溶组织梭菌ii型胶原酶及其制备方法和应用
CN113265412A (zh) * 2021-05-20 2021-08-17 北京化工大学 多酶偶联定位体系及其构建方法和应用
CN113393900A (zh) * 2021-06-09 2021-09-14 吉林大学 基于改进Transformer模型的RNA状态推断研究方法
CN113393900B (zh) * 2021-06-09 2022-08-02 吉林大学 基于改进Transformer模型的RNA状态推断研究方法
CN114672505A (zh) * 2022-03-15 2022-06-28 江苏省中国科学院植物研究所 一种功能性表达细胞色素p450酶的方法及其应用
CN114921463A (zh) * 2022-04-11 2022-08-19 中国农业科学院生物技术研究所 一种调控萜类化合物合成产量的人工非编码RNA分子CsiY及其应用
CN114921463B (zh) * 2022-04-11 2023-12-01 中国农业科学院生物技术研究所 一种调控萜类化合物合成产量的人工非编码RNA分子CsiY及其应用
CN115074410A (zh) * 2022-06-30 2022-09-20 武汉合生科技有限公司 一种生物合成虾青素的方法及载体
CN115992160A (zh) * 2022-09-30 2023-04-21 倍生生物科技(深圳)有限公司 一种穿梭载体及其构建方法和应用
CN116013407A (zh) * 2022-12-26 2023-04-25 浙江大学杭州国际科创中心 一种基于语言模型的性质解耦蛋白质生成方法
CN116504313A (zh) * 2023-05-08 2023-07-28 暨南大学 基因终止子查询方法、装置、设备及可读存储介质
CN117275590A (zh) * 2023-11-10 2023-12-22 华东师范大学 有机固废体系中大分子的降解功能基因数据库与分析平台
CN117275590B (zh) * 2023-11-10 2024-03-26 华东师范大学 有机固废体系中大分子的降解功能基因数据库与分析平台

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EP3485013A1 (en) 2019-05-22
US20200370058A1 (en) 2020-11-26
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