CN110934101A - Eriocheir sinensis parent genetic breeding method - Google Patents
Eriocheir sinensis parent genetic breeding method Download PDFInfo
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- CN110934101A CN110934101A CN201911389668.9A CN201911389668A CN110934101A CN 110934101 A CN110934101 A CN 110934101A CN 201911389668 A CN201911389668 A CN 201911389668A CN 110934101 A CN110934101 A CN 110934101A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
Description
Technical Field
The invention relates to a genetic breeding method of parent crabs, in particular to a genetic breeding method of parent crabs of eriocheir sinensis in Yangtze river origin producing areas, and belongs to the technical field of aquaculture.
Background
River crabs are outstanding in special aquatic products, not only have high nutritional value and delicious taste, but also have large market demand at home and abroad, and have short breeding period, fast growth and high benefit, so the area of domestic breeding areas is increased year by year. China is a Yangtze river eriocheir sinensis origin place, the breeding yield and the commodity quality of China are always reputed, and particularly, the international brand of the Yangtze river eriocheir sinensis is formed by the unique water area and climate advantages of the Yangtze river system, the continuous development of the river eriocheir sinensis breeding industry in recent years is driven, and the Yangtze river eriocheir sinensis breeding industry becomes a pillar industry for agricultural efficiency enhancement and farmer income increase in China.
However, in the development process, a plurality of adverse factors which restrict the further development of the river crab industry exist and need to be solved urgently: firstly, the river crab parents or offspring seeds come from Liaohe river system and the Yangtze river system, so that river crab germ plasm resources are mixed, and Chinese mitten crab germ plasm resources in the original place of the Yangtze river system are seriously damaged; meanwhile, many river crab parents bred in a breeding farm are inbred, and the phenomena of multi-generation hybrid and inbred breeding cause severe germplasm resource degradation, so that the river crab seedlings have poor quality, small breeding specifications, low survival rate, precocious puberty, reduced quality and low economic benefit. Therefore, in order to keep the healthy, continuous and rapid development of the river crab breeding industry in China and improve the quality and the product competitiveness of river crabs, the genetic breeding work of parent crabs in Yangtze river source of Chinese mitten crabs is very urgent when the high-quality original Chinese mitten crabs are cultured vigorously.
Disclosure of Invention
Aiming at the problems, the invention mainly aims at ① protecting parent crab germplasm resources of Chinese mitten crabs in the origin of Yangtze river in China. ② ensures that parent breeding of Chinese mitten crabs is high-quality and high-efficiency in production.
According to the technical problem to be solved, the invention designs the eriocheir sinensis parent genetic breeding method which sequentially comprises the following steps of ① seed selection, ② juvenile crab cultivation, ③ first-instar crab seed cultivation, ④ pond adult crab cultivation and ⑤ secondary genetic breeding parent adult crab.
As a preferred technical scheme of the invention, further, in the genetic breeding method of the eriocheir sinensis parents, the wild eriocheir sinensis in the Yangtze river basin origin place is selected in step ①, and the breeding standards of the parents are as follows:
and (3) appearance standard: 1. appearance: the back is cyan, the abdomen is grey white, and the golden claw is yellow; the back is covered with a layer of hard dorsal conch, the abdomen is 7 in total, the abdomen is bent to the front and is attached to the ventral surface of the head and chest; the abdomen of the female adult crab is round (reunion), the abdomen of the male adult crab is long and narrow triangle (sharp navel), and the appendages of the chest comprise l pairs of chela and 4 pairs of feet;
2. the motion characteristics are as follows: transversely crawling;
the quality requirement is as follows: 1. fresh degree: the shell, the chela and the foot are complete, the color quality is clear, no foreign matter is attached, and the action is quick; 2. odor: has special fishy and fresh taste.
In the eriocheir sinensis parent genetic breeding method, the young crabs in the step ② are specifically I-stage young crabs of which the appearance is close to that of adult crabs after one molting of megalops, III-stage young crabs after three molting, V-stage young crabs after five molting, live in a camp benthic place and have the specification of 6000 crabs/kg;
the cultivation method comprises the following specific steps:
1. culture pond conditions and facilities: the culture pond is close to a water source, the water quantity is abundant, and the water quality is fresh; anti-escape measures should be established around; the water depth is 0.8-1.2 m; the water quality meets the regulations of GB11607 and NY 5051; the shape is preferably rectangular in the north-south direction; preferably soil-clay loam;
2. preparation before seedling setting: cleaning pool and sterilizing, wherein the consumption of the Yongsheng lime for sterilizing a new pool is 0.2kg/m2(ii) a Arranging aquatic weeds, mainly comprising alternanthera philoxeroides, wherein the planting area is 50-60%, and the balance is waterweed; managing oxygenation equipment; fertilizing and cultivating water; adding fresh water, wherein the transparency is preferably 50 cm, when juvenile crabs go into the pond, the juvenile crabs mainly contain algae and simultaneously have rotifers and small cladocerans;
3. crab larva throwing: the germplasm is high-quality crab seedlings; the selection criteria are: strong constitution, strong vitality, golden yellow color, uniform size, and can be desalted for more than four days, with salinity below 3, and the age of the day for more than seven days; the gauze is required to be lightly and uniformly scattered in the net cage during transportation, and a layer of gauze is paved in the net cage to keep moist; when in stocking, the pond water is required to be drenched for 5 minutes, and after the crab larvae adapt to the water temperature, the mouth of the box is slowly opened, so that the crab larvae automatically disperse and swim away and never fall over;
4. dividing the young crabs into ponds: the young crabs which become V-stage after four-week cultivation can be transferred into the larval stage by the pond; the catching of the young crabs is mainly realized by flushing and fishing, and the young crabs are screened, classified and stocked in different ponds.
In the eriocheir sinensis parent genetic breeding method, one-year crabs in the step ③ are juvenile crabs, and specifically, juvenile crabs are bred for 150 days after raising for 120 plus 200 crabs with immature gonads per kilogram;
the cultivation method comprises the following specific steps:
1. breeding pool conditions and facilities: the breeding pond is preferably a pond or a rice field, the height of the escape-proof wall is more than 0.6m, the water quality and the substrate are the same as those of the young crabs, and the water depth is preferably 1.5-1.8 m;
2. preparation before stocking: cleaning pool and sterilizing, wherein the consumption of the Yongsheng lime for sterilizing a new pool is 0.2kg/m2(ii) a Arranging aquatic weeds, wherein the aquatic weeds mainly comprise alternanthera philoxeroides, and the planting area is about two thirds;
3. young crabs are put in stocking: the germ plasm is high-quality juvenile crab, the quality, the size and the specification are uniform, the appendage is complete, no disease exists, and the physique is strong; the stocking density is 40-60 per square meter, and the stocking method is that the young crabs can climb away by themselves by uniformly spreading along the periphery of the pond;
4. feeding the feed: selecting a compound feed with rich nutrition, wherein the feeding amount is 5 percent, and the compound feed is uniformly sprayed on a submerged belt around the pond;
5. water quality regulation and control: changing water by about ten centimeters every week, simultaneously adjusting the pH value, and carrying out measures of disinfection, bottom changing, bacterium supplementation and the like every week after 7 months;
6. daily management: the pond is patrolled in the morning and evening, the feeding, the activity, the shelling and the water quality change of the young crabs are observed, and the treatment measures are taken in time; establishing an escape-proof net to prevent the escape of the young crabs in rainy days; cleaning small green crabs in time;
7. catching the young crabs: the method of ground cage capture, light capture, dry pond capture promotion and the like is adopted to capture the crabs in the pond as much as possible.
In the genetic breeding method of the eriocheir sinensis parents, the parent mature crabs are further genetically bred in the step ⑤, parent eriocheir sinensis crabs in Yangtze river origin producing areas are adopted for reproduction, male eriocheir sinensis crabs in Yangtze river origin producing areas are respectively bred with F1 generation female crabs and female crabs in Yangtze river origin producing areas for reproduction and breeding, and crab species with strong germplasm resources, large specifications, strong disease resistance and high economic value are preferably packed;
the parent breeding standard specifically comprises the following steps:
and (3) appearance standard: the produced commercial crabs meet the requirements of 'green back, white belly, golden claw and yellow hair':
1. appearance: the back is cyan, the abdomen is grey white, and the golden paw is yellow. The back is covered with a layer of hard dorsal conch, the abdomen is 7 in total, the abdomen is bent to the front and is attached to the ventral surface of the head and chest; the abdomen of the female adult crab is round (reunion), the abdomen of the male adult crab is long and narrow triangle (sharp navel), and the appendages of the chest comprise l pairs of chela and 4 pairs of feet;
2. the motion characteristics are as follows: transverse crawling:
the quality requirement is as follows: 1. fresh degree: the shell, the chela and the foot are complete, the color quality is clear, no foreign matter is attached, and the action is quick;
2. odor: has special fishy and fresh taste.
The beneficial technical effects brought by the genetic breeding of the eriocheir sinensis parents are as follows:
(1) the invention aims to compare the genetic breeding of the parent of the eriocheir sinensis by using a scientific and efficient breeding technology, breeding management and water quality management through parent selection and hybridization technology, and forms the advantages of excellent parent line, pure germplasm resources and high yield through breeding and hybridization of high-quality parent, thereby being beneficial to the eriocheir sinensis industry;
(2) meanwhile, the female crabs after high-quality breeding and the male eriocheir sinensis in the Yangtze river origin are hybridized again, and the advantages of parents are fused after selection, so that the high-quality, high-efficiency and sustainable production of the eriocheir sinensis is ensured; meanwhile, the specification and the economic benefit of the adult crabs are improved, the occurrence probability of diseases is reduced, and the healthy and rapid development of the eriocheir sinensis industry is facilitated;
(3) the parent genetic breeding of the eriocheir sinensis has the effects of excellent parent line, pure germplasm resources, high immunity and disease defense capability and enhanced anti-stress capability.
(4) The invention improves the average finished product weight of the male crabs by 15 percent, improves the average finished product weight of the female crabs by 27 percent, and simultaneously improves the overall survival rate of the integral eriocheir sinensis crabs by more than 10 percent.
Detailed Description
The foregoing aspects of the present invention are described in further detail by way of examples, but it should not be construed that the scope of the above-described subject matter of the present invention is limited to the following examples, and that all the technologies implemented based on the above-described aspects of the present invention belong to the scope of the present invention.
Example 1
The embodiment provides a eriocheir sinensis parent genetic breeding method which sequentially comprises the following steps of ① seed selection, ② juvenile crab cultivation, ③ first-instar crab seed cultivation, ④ pond adult crab cultivation and ⑤ secondary genetic breeding parent adult crabs.
As the preferred technical scheme of the embodiment, further, the wild eriocheir sinensis in the Yangtze river basin origin place is selected in the step ①, and the breeding criteria of the parents are as follows:
and (3) appearance standard: 1. appearance: the back is cyan, the abdomen is grey white, and the golden claw is yellow; the back is covered with a layer of hard dorsal conch, the abdomen is 7 in total, the abdomen is bent to the front and is attached to the ventral surface of the head and chest; the abdomen of the female adult crab is round (reunion), the abdomen of the male adult crab is long and narrow triangle (sharp navel), and the appendages of the chest comprise l pairs of chela and 4 pairs of feet;
2. the motion characteristics are as follows: transversely crawling;
the quality requirement is as follows: 1. fresh degree: the shell, the chela and the foot are complete, the color quality is clear, no foreign matter is attached, and the action is quick; 2. odor: has special fishy and fresh taste.
In the eriocheir sinensis parent genetic breeding method, the young crabs in the step ② are specifically I-stage young crabs of which the appearance is close to that of adult crabs after one molting of megalops, III-stage young crabs after three molting, V-stage young crabs after five molting, live in a camp benthic place and have the specification of 6000 crabs/kg;
the cultivation method comprises the following specific steps:
1. culture pond conditions and facilities: the culture pond is close to a water source, the water quantity is abundant, and the water quality is fresh; anti-escape measures should be established around; the water depth is 0.8-1.2 m; the water quality meets the regulations of GB11607 and NY 5051; the shape is preferably rectangular in the north-south direction; preferably soil-clay loam;
2. preparation before seedling setting: cleaning pool and sterilizing, wherein the consumption of the Yongsheng lime for sterilizing a new pool is 0.2kg/m2(ii) a Arranging aquatic weeds, mainly comprising alternanthera philoxeroides, wherein the planting area is 50-60%, and the balance is waterweed; managing oxygenation equipment; fertilizing and cultivating water; adding fresh water, wherein the transparency is preferably 50 cm, when juvenile crabs go into the pond, the juvenile crabs mainly contain algae and simultaneously have rotifers and small cladocerans;
3. crab larva throwing: the germplasm is high-quality crab seedlings; the selection criteria are: strong constitution, strong vitality, golden yellow color, uniform size, and can be desalted for more than four days, with salinity below 3, and the age of the day for more than seven days; the gauze is required to be lightly and uniformly scattered in the net cage during transportation, and a layer of gauze is paved in the net cage to keep moist; when in stocking, the pond water is required to be drenched for 5 minutes, and after the crab larvae adapt to the water temperature, the mouth of the box is slowly opened, so that the crab larvae automatically disperse and swim away and never fall over;
4. dividing the young crabs into ponds: the young crabs which become V-stage after four-week cultivation can be transferred into the larval stage by the pond; the catching of the young crabs is mainly realized by flushing and fishing, and the young crabs are screened, classified and stocked in different ponds.
The first-instar crab in the step ③ is a juvenile crab, specifically, the juvenile crab is bred into 100-;
the cultivation method comprises the following specific steps:
1. breeding pool conditions and facilities: the breeding pond is preferably a pond or a rice field, the height of the escape-proof wall is more than 0.6m, the water quality and the substrate are the same as those of the young crabs, and the water depth is preferably 1.5-1.8 m;
2. preparation before stocking: cleaning pool and sterilizing, wherein the consumption of the Yongsheng lime for sterilizing a new pool is 0.2kg/m2(ii) a Arranging aquatic weeds, wherein the aquatic weeds mainly comprise alternanthera philoxeroides, and the planting area is about two thirds;
3. young crabs are put in stocking: the germ plasm is high-quality juvenile crab, the quality, the size and the specification are uniform, the appendage is complete, no disease exists, and the physique is strong; the stocking density is 40-60 per square meter, and the stocking method is that the young crabs can climb away by themselves by uniformly spreading along the periphery of the pond;
4. feeding the feed: selecting a compound feed with rich nutrition, wherein the feeding amount is 5 percent, and the compound feed is uniformly sprayed on a submerged belt around the pond;
5. water quality regulation and control: changing water by about ten centimeters every week, simultaneously adjusting the pH value, and carrying out measures of disinfection, bottom changing, bacterium supplementation and the like every week after 7 months;
6. daily management: the pond is patrolled in the morning and evening, the feeding, the activity, the shelling and the water quality change of the young crabs are observed, and the treatment measures are taken in time; establishing an escape-proof net to prevent the escape of the young crabs in rainy days; cleaning small green crabs in time;
7. catching the young crabs: the method of ground cage capture, light capture, dry pond capture promotion and the like is adopted to capture the crabs in the pond as much as possible.
In the step ⑤, the parent adult crabs are inherited again, parent crabs of the eriocheir sinensis in the Yangtze river origin place are adopted for reproduction, male eriocheir sinensis crabs in the Yangtze river origin place are respectively bred and bred with F1 generation female crabs and female crabs in the Yangtze river origin place, and crab species with strong germplasm resources, large specifications, strong disease resistance and high economic value are preferably packed;
the parent breeding standard specifically comprises the following steps:
and (3) appearance standard: the produced commercial crabs meet the requirements of 'green back, white belly, golden claw and yellow hair':
1. appearance: the back is cyan, the abdomen is grey white, and the golden paw is yellow. The back is covered with a layer of hard dorsal conch, the abdomen is 7 in total, the abdomen is bent to the front and is attached to the ventral surface of the head and chest; the abdomen of the female adult crab is round (reunion), the abdomen of the male adult crab is long and narrow triangle (sharp navel), and the appendages of the chest comprise l pairs of chela and 4 pairs of feet;
2. the motion characteristics are as follows: transverse crawling:
the quality requirement is as follows: 1. fresh degree: the shell, the chela and the foot are complete, the color quality is clear, no foreign matter is attached, and the action is quick;
2. odor: has special fishy and fresh taste.
In this embodiment, the following comparative examples 1 to 3 are selected, and the technical effects of this embodiment are compared correspondingly:
comparative example 1
The difference from the experimental example 1 is that the eriocheir sinensis in the step (4) is a Liaohe water system; (ii) a
Comparative example 2
The difference from the experimental example 1 is that the specification of the eriocheir sinensis in the Yangtze river origin in the step (4) is 6 male, 3..5 female;
comparative example 3
The difference from the experimental example 1 is that in the step (9), the F1 generation is not hybridized with the Eriocheir sinensis in the Yangtze river origin, but the filial generation is hybridized;
of course, the above description is not intended to limit the present invention, and the present invention is not limited to the above examples. Those skilled in the art should also realize that such changes, modifications, additions and substitutions are within the true spirit of the invention.
Claims (5)
2. The eriocheir sinensis parent genetic breeding method according to claim 1, wherein the steps compriseThe method selects the wild eriocheir sinensis in the origin of Yangtze river basin, and the breeding standard of the parents is as follows:
and (3) appearance standard: 1. appearance: the back is cyan, the abdomen is grey white, and the golden claw is yellow; the back is covered with a layer of hard dorsal conch, the abdomen is 7 in total, the abdomen is bent to the front and is attached to the ventral surface of the head and chest; the abdomen of the female adult crab is round (reunion), the abdomen of the male adult crab is long and narrow triangle (sharp navel), and the appendages of the chest comprise l pairs of chela and 4 pairs of feet;
2. the motion characteristics are as follows: transversely crawling;
the quality requirement is as follows: 1. fresh degree: the shell, the chela and the foot are complete, the color quality is clear, no foreign matter is attached, and the action is quick; 2. odor: has special fishy and fresh taste.
3. The eriocheir sinensis parent genetic breeding method according to claim 1, wherein the steps compriseThe young crab in the crab pot is specifically as follows: the megalopas are transformed into I-stage young crabs with the appearance close to that of adult crabs through one molt; carrying out three molting processes to obtain juvenile crabs in stage III; five molting processes are taken to obtain the V-stage young crabs which live in the benthic environment with the specification of 5000-;
the cultivation method comprises the following specific steps:
1. culture pond conditions and facilities: the culture pond is close to a water source, the water quantity is abundant, and the water quality is fresh; anti-escape measures should be established around; the water depth is 0.8-1.2 m; the water quality meets the regulations of GB11607 and NY 5051; the shape is preferably rectangular in the north-south direction; preferably soil-clay loam;
2. preparation before seedling setting: cleaning pool and sterilizing, wherein the consumption of the Yongsheng lime for sterilizing a new pool is 0.2kg/m2(ii) a Arranging aquatic weeds, mainly comprising alternanthera philoxeroides, wherein the planting area is 50-60%, and the balance is waterweed; managing oxygenation equipment; fertilizing and cultivating water; adding fresh water, wherein the transparency is preferably 50 cm, when juvenile crabs go into the pond, the juvenile crabs mainly contain algae and simultaneously have rotifers and small cladocerans;
3. crab larva throwing: the germplasm is high-quality crab seedlings; the selection criteria are: strong constitution, strong vitality, golden yellow color, uniform size, and can be desalted for more than four days, with salinity below 3, and the age of the day for more than seven days; the gauze is required to be lightly and uniformly scattered in the net cage during transportation, and a layer of gauze is paved in the net cage to keep moist; when in stocking, the pond water is required to be drenched for 5 minutes, and after the crab larvae adapt to the water temperature, the mouth of the box is slowly opened, so that the crab larvae automatically disperse and swim away and never fall over;
4. dividing the young crabs into ponds: the young crabs which become V-stage after four-week cultivation can be transferred into the larval stage by the pond; the catching of the young crabs is mainly realized by flushing and fishing, and the young crabs are screened, classified and stocked in different ponds.
4. The eriocheir sinensis parent genetic breeding method according to claim 1, wherein the steps compriseThe first instar crab is juvenile crab, which comprises the following specific steps: the young crabs are raised for 150 days after 120-;
the cultivation method comprises the following specific steps:
1. breeding pool conditions and facilities: the breeding pond is preferably a pond or a rice field, the height of the escape-proof wall is more than 0.6m, the water quality and the substrate are the same as those of the young crabs, and the water depth is preferably 1.5-1.8 m;
2. preparation before stocking: cleaning pool and sterilizing, wherein the consumption of the Yongsheng lime for sterilizing a new pool is 0.2kg/m2(ii) a Arranging aquatic weeds, wherein the aquatic weeds mainly comprise alternanthera philoxeroides, and the planting area is about two thirds;
3. young crabs are put in stocking: the germ plasm is high-quality juvenile crab, the quality, the size and the specification are uniform, the appendage is complete, no disease exists, and the physique is strong; the stocking density is 40-60 per square meter, and the stocking method is that the young crabs can climb away by themselves by uniformly spreading along the periphery of the pond;
4. feeding the feed: selecting a compound feed with rich nutrition, wherein the feeding amount is 5 percent, and the compound feed is uniformly sprayed on a submerged belt around the pond;
5. water quality regulation and control: changing water by about ten centimeters every week, simultaneously adjusting the pH value, and carrying out measures of disinfection, bottom changing, bacterium supplementation and the like every week after 7 months;
6. daily management: the pond is patrolled in the morning and evening, the feeding, the activity, the shelling and the water quality change of the young crabs are observed, and the treatment measures are taken in time; establishing an escape-proof net to prevent the escape of the young crabs in rainy days; cleaning small green crabs in time;
7. catching the young crabs: the method of ground cage capture, light capture, dry pond capture promotion and the like is adopted to capture the crabs in the pond as much as possible.
5. The eriocheir sinensis parent genetic breeding method according to claim 1, wherein the steps compriseThe parent adult crabs are bred in the middle and secondary inheritance mode, parent crabs of the eriocheir sinensis in the Yangtze river origin place are adopted for reproduction, male eriocheir sinensis crabs in the Yangtze river origin place are respectively bred with F1 generation female crabs and female crabs in the Yangtze river origin place, and crab species with strong germplasm resources, large specifications, strong disease resistance and high economic value are preferably packed;
the parent breeding standard specifically comprises the following steps:
and (3) appearance standard: the produced commercial crabs meet the requirements of 'green back, white belly, golden claw and yellow hair':
1. appearance: the back is cyan, the abdomen is grey white, the golden claw is yellow, the back is covered with a layer of hard tergum, the abdomen is 7 in total, the abdomen is bent to the front, and the golden claw is attached to the abdomen of the head and chest; the abdomen of the female adult crab is round (reunion), the abdomen of the male adult crab is long and narrow triangle (sharp navel), and the appendages of the chest comprise l pairs of chela and 4 pairs of feet;
2. the motion characteristics are as follows: transverse crawling:
the quality requirement is as follows: 1. fresh degree: the shell, the chela and the foot are complete, the color quality is clear, no foreign matter is attached, and the action is quick;
2. odor: has special fishy and fresh taste.
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CN104982362A (en) * | 2015-06-24 | 2015-10-21 | 中国水产科学研究院黄海水产研究所 | Breeding method of portunus trituberculatus rapid-growth novel variety |
CN105918173A (en) * | 2016-05-03 | 2016-09-07 | 中国水产科学研究院黄海水产研究所 | Efficient breeding method for families of Portunus trituberculatus |
CN106172138A (en) * | 2016-08-02 | 2016-12-07 | 盘锦光合蟹业有限公司 | A kind of crab selection |
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